CN101717309B - Culture medium for straw rotting edible fungi solid strain and method for preparing solid strain - Google Patents
Culture medium for straw rotting edible fungi solid strain and method for preparing solid strain Download PDFInfo
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- CN101717309B CN101717309B CN2009102729284A CN200910272928A CN101717309B CN 101717309 B CN101717309 B CN 101717309B CN 2009102729284 A CN2009102729284 A CN 2009102729284A CN 200910272928 A CN200910272928 A CN 200910272928A CN 101717309 B CN101717309 B CN 101717309B
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Abstract
The invention relates to a culture medium for a straw rotting edible fungi solid strain and a method for preparing the solid strain, which belong to the field of strain culture. The method comprises the following steps: firstly, preparing enough materials of peat soil, cotton seed hulls, bran, gypsum and cane sugar according to a formula proportion; secondly, adding lime and water, mixing and stirring the lime and the water with the materials to prepare the culture medium with the pH value of 6.5 to 7.0; thirdly, sub-packing the culture medium with strain bags with 0.25 kilogram for each bag, and then performing high-temperature sterilization for 2.5 hours at the temperature of between 121 and 123 DEG C; and fourthly, after the strain bags are cooled, inoculating a parent seed or a stock seed, then putting the strain bags into a darkroom at the temperature of 25 DEG C to culture mycelia, clearing dirt and eliminating impurities after culturing the mycelia for 7 days, and fully filling the bags with the mycelia after culturing the mycelia for 25 to 30 days to obtain the solid strain. The culture medium prepared in the invention and the solid strain prepared by using the culture medium not only are difficult to age and have long storage lives, but also have simple manufacturing processes and low requirements on sterilization, and save more cost.
Description
Technical field
The present invention relates to the solid spawn of a kind of edible mushrooms, relate in particular to substratum of the rotten edible mushrooms solid spawn of a kind of grass and the preparation method of solid spawn, specifically be applicable to the spawn culture of careless rotten edible mushrooms.
Background technology
The rotten edible mushrooms of grass comprises edible mushroomss such as Twospore Mushroom, Brazilian mushroom, straw mushroom, Coprinus comatus, brown mushroom.For a long time, careless rotten edible mushrooms uses solid particulates such as wheat, grain as bacterium culture medium more, and the bacterial classification of prepared production, cultivation usefulness is called as kernel culture; Though having, kernel culture sprouts advantages such as fast, that vegetative point is many and growth potential is strong; But its manufacture craft is loaded down with trivial details, time-consuming, and sterilization requires height, bacterial classification to pollute difficult control, and the most serious is the easy catabiosis of telling yellow water of bacterial classification; Thereby cause preservation period shorter, be generally 35～45 days.
Summary of the invention
The objective of the invention is to overcome that the manufacture craft that exists in the prior art is loaded down with trivial details, sterilization requires high, bacterial classification to be prone to defective and problem aging and that preservation period is short; Provide that a kind of technology is simple, sterilization require low, bacterial classification is difficult aging, and the preservation period substratum of the long rotten edible mushrooms solid spawn of a kind of grass and the preparation method of solid spawn.
For realizing above purpose; Technical solution of the present invention is: the substratum of the rotten edible mushrooms solid spawn of a kind of grass; The component of this substratum and weight part ratio thereof are: peat soil 68～78, cotton seed hulls 10～15, wheat bran 10～15; Gypsum 1～2, sucrose 1～2 and the pH value that adds lime adjustment substratum are 6.5～7.0.
The component of said substratum and weight part ratio thereof are: peat soil 73, and cotton seed hulls 15, wheat bran 10, gypsum 1, sucrose 1 and the pH value that adds lime adjustment substratum are 7.0.
A kind of preparation method who adopts the careless rotten edible mushrooms solid spawn of said substratum is characterized in that carrying out as follows:
(1) get the raw materials ready: prepare enough raw material by turnout, and peat soil sieved to remove the foreign material of diameter greater than 1cm, require wheat bran fresh, do not have and go mouldy, cotton seed hulls does not lump agglomerating;
(2) preparation: in the component of substratum and weight part ratio thereof with peat soil, cotton seed hulls, wheat bran, gypsum and sucrose mixing; And add entry; The weight ratio of raw material and water is 1: 1.2～1: 1.3; Add behind the water raw material mixed and turn so that the raw material suction evenly, adds lime simultaneously with adjustment pH value, the pH value of the feasible substratum that makes is 6.5～7.0;
(3) packing: the above-mentioned substratum that makes is carried out packing with strain bag; Every packed substratum 0.25kg; Strain bag after the requirement packing is full, degree of tightness suitable; And on strain bag, add a cover plastic ferrule and seal, note handling with care when strain bag is contacted and cause bacterial classification to pollute in case strain bag is damaged;
(4) sterilization: the above-mentioned strain bag that makes is carried out high-temperature sterilization, and sterilising temp is 121 ℃～123 ℃, and the time is 2.5 hours, and sterilization is put into the indoor naturally cooling that carries out with subsequent use with strain bag after finishing;
(5) inoculation: in Bechtop or inoculation tank, open the seal closure of the strain bag after the above-mentioned sterilization, the substratum that makes is carried out aseptic inoculation; When making original seed, it is the square fritter of 1cm that the mother of every bag of access plants consumption; When making production was planted, the original seed consumption of every bag of access was 15ml;
(6) cultivate: being put in postvaccinal strain bag in 25 ℃ the darkroom and cultivating mycelia, be mother's kind like what insert, then field planting material feeding after 5 days, as access be original seed, then field planting material feeding after 3 days; Cultivate the mycelia impurity elimination of can removing contamination after 7 days, cultivate mycelia mycelia purseful after 25～30 days, bacterial classification is ripe, promptly makes described solid spawn.
Used strain bag is an individual layer high-pressure polypropylene strain bag in the said packing step.
Compared with prior art, beneficial effect of the present invention is:
Since among the preparation method of the substratum of the rotten edible mushrooms solid spawn of a kind of grass of the present invention and solid spawn employed raw material include only peat soil, cotton seed hulls, wheat bran, gypsum and sucrose, and the lime of adjustment pH value is therefore when making is of the present invention; Need not soak or boiling, and general kernel culture must soak or boiling wheat when making, otherwise wheat is difficult to the water cut that reaches suitable; And soaking, will get hold of the duration and degree of heating during boiling, careless slightlyly then leading to the failure, in addition; The container containing of kernel culture is generally vial; Volume weight is big, uses inconvenience, and its used strain bag requires to be double-ply bag; Operation is loaded down with trivial details, time-consuming, is the individual layer strain bag and the present invention uses.So simple, easy to operate, saving of work and time of technology of the present invention.
Since among the preparation method of the substratum of the rotten edible mushrooms solid spawn of a kind of grass of the present invention and solid spawn employed raw material include only peat soil, cotton seed hulls, wheat bran, gypsum and sucrose; And the lime of adjustment pH value; And the nutrition of the general employed raw material of kernel culture is extremely abundant; Very easily cause pollution, therefore long to the sterilization time of kernel culture, generally more than 4 hours; And sterilization time of the present invention was generally 2.5 hours, and therefore sterilization of the present invention requires low, the difficult pollution.
3. because substratum of the rotten edible mushrooms solid spawn of a kind of grass of the present invention and the prepared solid spawn preservation period of preparation method of solid spawn are 60～70 days; And general kernel culture is prone to wear out; Promptly can tell the yellow water phenomenon in mycelium culture after 30 days, therefore preservation period of the present invention is longer.
Since among the preparation method of the substratum of the rotten edible mushrooms solid spawn of a kind of grass of the present invention and solid spawn employed main raw material be peat soil; Peat soil forms through spontaneous fermentation for many years, and rotten degree is good, and retention ability is strong; Fertility is suitable; China's peat soil aboundresources in addition, low price, the peat soil price is 250～300 yuan/m
3, be converted into 0.25～0.30 yuan/liter, every liter of raw material can be made 2 bottles of solid spawns; And the raw materials cost of same volumetrical kernel culture is 0.40～0.50 yuan; Thereby adopting peat soil is that major ingredient is made solid spawn, and its raw materials cost reduces about 40% than kernel culture; Need not pre-treatment just can directly use owing to peat soil in addition; And then make manufacture craft simplify, produce expenses such as required water, electricity, manual work to reduce, so the bacterial classification of the present invention's making reduces production costs 50% at least than kernel culture, so the present invention saves cost.
Embodiment
Describe the present invention below in conjunction with embodiment:
The substratum of the rotten edible mushrooms solid spawn of a kind of grass of the present invention; The component of this substratum and weight part ratio thereof are: peat soil 68～78, cotton seed hulls 10～15, wheat bran 10～15; Gypsum 1～2, sucrose 1～2 and the pH value that adds lime adjustment substratum are 6.5～7.0.
The component of said substratum and weight part ratio thereof are: peat soil 73, and cotton seed hulls 15, wheat bran 10, gypsum 1, sucrose 1 and the pH value that adds lime adjustment substratum are 7.0.
The moity and the Action Specification of above-mentioned substratum are following:
Peat soil: as the major ingredient in the solid spawn of the present invention, it forms through spontaneous fermentation for many years, and rotten degree is good, and retention ability is strong; Fertility is suitable, contains a large amount organic matter, is 65.4% like the organic average content of the peat soil in Chinese Jilin Province; Chuanxibei Plateau is 57.5%, and the content of nitrogen is also quite abundant in the peat soil, the Northeast's peat soil nitrogen content average out to 1.5～2.0%; Reach as high as 2.5%, in addition, the content of phosphorus also is higher than the mineral substance soil body in the peat soil; About 0.29～0.33%, be very suitable for as the straw rotting fungus class, like the fruiting casingmaterial of Twospore Mushroom, straw mushroom, Brazilian mushroom etc.
Cotton seed hulls: graininess is good, can strengthen the ventilation property of culture material, in order to mycelial growth.
Wheat bran: for culture material provides VITAMINs and nitrogenous source.
Sucrose: for culture material provides quick-acting carbon sources.
Gypsum: be the culture material supplementing calcium element.
Lime: be culture material supplementing calcium element and adjustment pH value.
The present invention and then proposed a kind of preparation method who adopts the careless rotten edible mushrooms solid spawn of said substratum, this method may further comprise the steps successively:
(1) get the raw materials ready: prepare enough raw material by turnout, and peat soil sieved to remove the foreign material of diameter greater than 1cm, require wheat bran fresh, do not have and go mouldy, cotton seed hulls does not lump agglomerating;
(2) preparation: in the component of substratum and weight part ratio thereof with peat soil, cotton seed hulls, wheat bran, gypsum and sucrose mixing; And add entry; The weight ratio of raw material and water is 1: 1.2～1: 1.3; Add behind the water raw material mixed and turn so that the raw material suction evenly, adds lime simultaneously with adjustment pH value, the pH value of the feasible substratum that makes is 6.5～7.0;
(3) packing: the above-mentioned substratum that makes is carried out packing with strain bag; Every packed substratum 0.25kg; Strain bag after the requirement packing is full, degree of tightness suitable; And on strain bag, add a cover plastic ferrule and seal, note handling with care when strain bag is contacted and cause bacterial classification to pollute in case strain bag is damaged;
(4) sterilization: the above-mentioned strain bag that makes is carried out high-temperature sterilization, and sterilising temp is 121 ℃～123 ℃, and the time is 2.5 hours, and sterilization is put into the indoor naturally cooling that carries out with subsequent use with strain bag after finishing;
(5) inoculation: in Bechtop or inoculation tank, open the seal closure of the strain bag after the above-mentioned sterilization, the substratum that makes is carried out aseptic inoculation; When making original seed, it is the square fritter of 1cm that the mother of every bag of access plants consumption; When making production was planted, the original seed consumption of every bag of access was 15ml;
(6) cultivate: being put in postvaccinal bacterium bag in 25 ℃ the darkroom and cultivating mycelia, be mother's kind like what insert, then field planting material feeding after 5 days, as access be original seed, then field planting material feeding after 3 days; Cultivate the mycelia impurity elimination of can removing contamination after 7 days, cultivate mycelia mycelia purseful after 25～30 days, bacterial classification is ripe, promptly makes described solid spawn.
Used strain bag is an individual layer high-pressure polypropylene strain bag in the said packing step.
Plant for female like what in substratum, insert, then cultivation is covered with mycelia and is promptly got original seed, is also referred to as the secondary kind.
Like the original seed that is that in substratum, inserts, then cultivation is covered with mycelia and is promptly got cultivar, is also referred to as three grades of kinds, produces and plant or cultivar.
Embodiment 1:
The substratum of the solid spawn of the rotten edible mushrooms of a kind of grass, the component of this substratum and weight part ratio thereof are: peat soil 68, cotton seed hulls 15, wheat bran 15, gypsum 1, sucrose 1 and the pH value that adds lime adjustment substratum are 7.0.
A kind of preparation method who adopts the careless rotten edible mushrooms solid spawn of said substratum, this making method is carried out as follows:
(1) get the raw materials ready: prepare enough raw material by turnout, and peat soil sieved to remove the foreign material of diameter greater than 1cm, require wheat bran fresh, do not have and go mouldy, cotton seed hulls does not lump agglomerating;
(2) preparation: in the component of substratum and weight part ratio thereof with peat soil, cotton seed hulls, wheat bran, gypsum and sucrose mixing; And add entry; The weight ratio of raw material and water is 1: 1.2～1: 1.3; Add behind the water raw material mixed and turn so that the raw material suction evenly, adds lime simultaneously with adjustment pH value, the pH value of the feasible substratum that makes is 7.0;
(3) packing: the above-mentioned substratum that makes is carried out packing with strain bag; Every packed substratum 0.25kg; Strain bag after the requirement packing is full, degree of tightness suitable; And on strain bag, add a cover plastic ferrule and seal, note handling with care when strain bag is contacted and cause bacterial classification to pollute in case strain bag is damaged;
(4) sterilization: the above-mentioned strain bag that makes is carried out high-temperature sterilization, and sterilising temp is 121 ℃～123 ℃, and the time is 2.5 hours, and sterilization is put into the indoor naturally cooling that carries out with subsequent use with strain bag after finishing;
(5) inoculation: in Bechtop or inoculation tank, open the seal closure of the strain bag after the above-mentioned sterilization, the substratum in the strain bag carried out aseptic inoculation, access be female kind, it is the square fritter of 1cm that the mother of every bag of access plants consumption.
(6) cultivate: be put in postvaccinal bacterium bag in 25 ℃ the darkroom and cultivate mycelia, the mycelia impurity elimination of can removing contamination after 7 days is cultivated in field planting material feeding after 5 days, cultivation mycelia mycelia purseful after 25～30 days, and the bacterial classification maturation promptly makes described solid spawn.
Embodiment 2:
The substratum of the solid spawn of the rotten edible mushrooms of a kind of grass, the component of this substratum and weight part ratio thereof are: peat soil 78, cotton seed hulls 10, wheat bran 10, gypsum 1, sucrose 1 and the pH value that adds lime adjustment substratum are 6.8.
A kind of preparation method who adopts the careless rotten edible mushrooms solid spawn of said substratum, this making method is carried out as follows:
(1) get the raw materials ready: prepare enough raw material by turnout, and peat soil sieved to remove the foreign material of diameter greater than 1cm, require wheat bran fresh, do not have and go mouldy, cotton seed hulls does not lump agglomerating;
(2) preparation: in the component of substratum and weight part ratio thereof with peat soil, cotton seed hulls, wheat bran, gypsum and sucrose mixing; And add entry; The weight ratio of raw material and water is 1: 1.2～1: 1.3; Add behind the water raw material mixed and turn so that the raw material suction evenly, adds lime simultaneously with adjustment pH value, the pH value of the feasible substratum that makes is 6.8;
(3) packing: the above-mentioned substratum that makes is carried out packing with strain bag; Every packed substratum 0.25kg; Strain bag after the requirement packing is full, degree of tightness suitable; And on strain bag, add a cover plastic ferrule and seal, note handling with care when strain bag is contacted and cause bacterial classification to pollute in case strain bag is damaged;
(4) sterilization: the above-mentioned strain bag that makes is carried out high-temperature sterilization, and sterilising temp is 121 ℃～123 ℃, and the time is 2.5 hours, and sterilization is put into the indoor naturally cooling that carries out with subsequent use with strain bag after finishing;
(5) inoculation: in Bechtop or inoculation tank, open the seal closure of the strain bag after the above-mentioned sterilization, the substratum in the strain bag carried out aseptic inoculation, access be original seed, the original seed consumption of every bag of access is 15ml.
(6) cultivate: be put in postvaccinal bacterium bag in 25 ℃ the darkroom and cultivate mycelia, the mycelia impurity elimination of can removing contamination after 7 days is cultivated in field planting material feeding after 3 days, cultivation mycelia mycelia purseful after 25～30 days, and the bacterial classification maturation promptly makes described solid spawn.
Embodiment 3:
The substratum of the solid spawn of the rotten edible mushrooms of a kind of grass, the component of this substratum and weight part ratio thereof are: peat soil 72, cotton seed hulls 12, wheat bran 13, gypsum 1, sucrose 2 and the pH value that adds lime adjustment substratum are 6.5.
A kind of preparation method who adopts the careless rotten edible mushrooms solid spawn of said substratum, this making method is carried out as follows:
(1) get the raw materials ready: prepare enough raw material by turnout, and peat soil sieved to remove the foreign material of diameter greater than 1cm, require wheat bran fresh, do not have and go mouldy, cotton seed hulls does not lump agglomerating;
(2) preparation: in the component of substratum and weight part ratio thereof with peat soil, cotton seed hulls, wheat bran, gypsum and sucrose mixing; And add entry; The weight ratio of raw material and water is 1: 1.2～1: 1.3; Add behind the water raw material mixed and turn so that the raw material suction evenly, adds lime simultaneously with adjustment pH value, the pH value of the feasible substratum that makes is 6.5;
(3) packing: the above-mentioned substratum that makes is carried out packing with strain bag; Every packed substratum 0.25kg; Strain bag after the requirement packing is full, degree of tightness suitable; And on strain bag, add a cover plastic ferrule and seal, note handling with care when strain bag is contacted and cause bacterial classification to pollute in case strain bag is damaged;
(4) sterilization: the above-mentioned strain bag that makes is carried out high-temperature sterilization, and sterilising temp is 121 ℃～123 ℃, and the time is 2.5 hours, and sterilization is put into the indoor naturally cooling that carries out with subsequent use with strain bag after finishing;
(5) inoculation: in Bechtop or inoculation tank, open the seal closure of the strain bag after the above-mentioned sterilization, the substratum in the strain bag carried out aseptic inoculation, access be female kind, it is the square fritter of 1cm that the mother of every bag of access plants consumption.
(6) cultivate: be put in postvaccinal bacterium bag in 25 ℃ the darkroom and cultivate mycelia, the mycelia impurity elimination of can removing contamination after 7 days is cultivated in field planting material feeding after 5 days, cultivation mycelia mycelia purseful after 25～30 days, and the bacterial classification maturation promptly makes described solid spawn.
Embodiment 4:
The substratum of the solid spawn of the rotten edible mushrooms of a kind of grass, the component of this substratum and weight part ratio thereof are: peat soil 73, cotton seed hulls 15, wheat bran 10, gypsum 1, sucrose 1 and the pH value that adds lime adjustment substratum are 7.0.
A kind of preparation method who adopts the careless rotten edible mushrooms solid spawn of said substratum, this making method is carried out as follows:
(1) get the raw materials ready: prepare enough raw material by turnout, and peat soil sieved to remove the foreign material of diameter greater than 1cm, require wheat bran fresh, do not have and go mouldy, cotton seed hulls does not lump agglomerating;
(2) preparation: in the component of substratum and weight part ratio thereof with peat soil, cotton seed hulls, wheat bran, gypsum and sucrose mixing; And add entry; The weight ratio of raw material and water is 1: 1.2～1: 1.3; Add behind the water raw material mixed and turn so that the raw material suction evenly, adds lime simultaneously with adjustment pH value, the pH value of the feasible substratum that makes is 7.0;
(3) packing: the above-mentioned substratum that makes is carried out packing with strain bag; Every packed substratum 0.25kg; Strain bag after the requirement packing is full, degree of tightness suitable; And on strain bag, add a cover plastic ferrule and seal, note handling with care when strain bag is contacted and cause bacterial classification to pollute in case strain bag is damaged;
(4) sterilization: the above-mentioned strain bag that makes is carried out high-temperature sterilization, and sterilising temp is 121 ℃～123 ℃, and the time is 2.5 hours, and sterilization is put into the indoor naturally cooling that carries out with subsequent use with strain bag after finishing;
(5) inoculation: in Bechtop or inoculation tank, open the seal closure of the strain bag after the above-mentioned sterilization, the substratum in the strain bag carried out aseptic inoculation, access be original seed, the original seed consumption of every bag of access is 15ml;
(6) cultivate: be put in postvaccinal bacterium bag in 25 ℃ the darkroom and cultivate mycelia, the mycelia impurity elimination of can removing contamination after 7 days is cultivated in field planting material feeding after 3 days, cultivation mycelia mycelia purseful after 25～30 days, and the bacterial classification maturation promptly makes described solid spawn.
Substratum of the rotten edible mushrooms solid spawn of a kind of grass of the present invention and the preparation method of solid spawn are suitable for the rotten edible mushrooms of various grass such as Twospore Mushroom, straw mushroom, Coprinus comatus, brown mushroom, Brazilian mushroom is the cultivation of edible mushroomss such as Agaricus blazei Murrill.
General kernel culture is promptly told yellow water in mycelium culture after 30 days, it is old and feeble to show that its mycelia is prone to, not storage endurance; And the solid spawn of adopting making method of the present invention to make does not see that telling the yellow water phenomenon takes place, and its bacterial classification quality guaranteed period can reach 60～70 days, and its after planting mycelium germination, field planting aspect no significant difference; Sporophore can both normally form; Its quality product, output are suitable, and through 5 standard baskets of test, every basket of area is 0.205m
2, the result produces Brazilian mushroom fresh weight 9.18Kg, amounts to per unit area yield and is calculated as: 9.18Kg ÷ (0.205m2/ basket * 5 basket)=8.95Kg/m2, product quality indicator is no abnormal.
Following table is for being the applicable cases that example is come more various cultivation strains with the Brazilian mushroom:
Four kinds of Brazilian mushroom cultivar applicable cases relatively
Claims (4)
1. the substratum of the rotten edible mushrooms solid spawn of grass; It is characterized in that: the component of this substratum and weight part ratio thereof are: peat soil 68～78, cotton seed hulls 10～15, wheat bran 10～15; Gypsum 1～2, sucrose 1～2 and the pH value that adds lime adjustment substratum are 6.5～7.0.
2. the substratum of the rotten edible mushrooms solid spawn of a kind of grass according to claim 1; It is characterized in that: the component of said substratum and weight part ratio thereof are: peat soil 73, cotton seed hulls 15, wheat bran 10; Gypsum 1, sucrose 1 and the pH value that adds lime adjustment substratum are 7.0.
3. method that adopts claim 1 or 2 said medium preparation solid spawns is characterized in that carrying out as follows:
(1) get the raw materials ready: prepare enough raw material by turnout, and peat soil sieved to remove the foreign material of diameter greater than 1cm, require wheat bran fresh, do not have and go mouldy, cotton seed hulls does not lump agglomerating;
(2) preparation: in the component of substratum and weight part ratio thereof with peat soil, cotton seed hulls, wheat bran, gypsum and sucrose mixing; And add entry; The weight ratio of raw material and water is 1:1.2～1:1.3; Add behind the water raw material mixed and turn so that the raw material suction evenly, adds lime simultaneously with adjustment pH value, the pH value of the feasible substratum that makes is 6.5～7.0;
(3) packing: the above-mentioned substratum that makes is carried out packing with strain bag; Every packed substratum 0.25kg; Strain bag after the requirement packing is full, degree of tightness suitable; And on strain bag, add a cover plastic ferrule and seal, note handling with care when strain bag is contacted and cause bacterial classification to pollute in case strain bag is damaged;
(4) sterilization: the above-mentioned strain bag that makes is carried out high-temperature sterilization, and sterilising temp is 121 ℃～123 ℃, and the time is 2.5 hours, and sterilization is put into the indoor naturally cooling that carries out with subsequent use with strain bag after finishing;
(5) inoculation: in Bechtop or inoculation tank, open the seal closure of the strain bag after the above-mentioned sterilization, the substratum that makes is carried out aseptic inoculation; When making original seed, it is the square fritter of 1cm that the mother of every bag of access plants consumption; When making production was planted, the original seed consumption of every bag of access was 15ml;
(6) cultivate: being put in postvaccinal strain bag in 25 ℃ the darkroom and cultivating mycelia, is mother's kind like what insert, then field planting material feeding after 5 days; Like what insert is original seed, then field planting material feeding after 3 days; Cultivate the mycelia impurity elimination of can removing contamination after 7 days, cultivate mycelia mycelia purseful after 25～30 days, bacterial classification is ripe, promptly makes described solid spawn.
4. a kind of method that adopts the medium preparation solid spawn according to claim 3 is characterized in that: used strain bag is an individual layer high-pressure polypropylene strain bag in the said packing step.
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| CN103004453A (en) * | 2011-09-20 | 2013-04-03 | 杭州海智生物技术有限公司 | Manufacturing method of edible fungi cultivar and culture medium manufacturing raw materials for edible fungi cultivar |
| CN105039186B (en) * | 2013-12-24 | 2018-09-21 | 重庆市农业科学院 | A kind of fungi culture medium suitable for large-scale production |
| CN104926512A (en) * | 2015-06-19 | 2015-09-23 | 桂林健成生物科技开发有限公司 | Application of siraitia grosvenorii residue to production of solid spawn of edible fungi |
| CN104926511A (en) * | 2015-06-19 | 2015-09-23 | 桂林健成生物科技开发有限公司 | Application of post-sprout-harvesting seed husks/embryoid bodies and rhizome substrate in preparation of solid spawn of edible fungi |
| CN107299061A (en) * | 2017-08-08 | 2017-10-27 | 青岛科海生物有限公司 | The culture medium and its cultural method of a kind of itaconic acid producing strain |
| CN109089734A (en) * | 2018-08-31 | 2018-12-28 | 成都市宁升绿康食品有限公司 | Seafood mushroom cultivation strain bag preparation process |
| CN114175968A (en) * | 2021-11-11 | 2022-03-15 | 鲁东大学 | Composite material based on wood rotting fungus mycelia and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1918961A (en) * | 2005-08-22 | 2007-02-28 | 郭志忠 | Method for producing edible fungi by peat culture medium |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1918961A (en) * | 2005-08-22 | 2007-02-28 | 郭志忠 | Method for producing edible fungi by peat culture medium |
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