CN106399123B - Method for quickly producing seeds of edible fungi - Google Patents
Method for quickly producing seeds of edible fungi Download PDFInfo
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- 238000004519 manufacturing process Methods 0.000 claims abstract description 49
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- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 14
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 14
- 239000008223 sterile water Substances 0.000 claims abstract description 12
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- 238000011081 inoculation Methods 0.000 claims description 21
- 230000001580 bacterial effect Effects 0.000 claims description 19
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 241000894007 species Species 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 6
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- 229920001817 Agar Polymers 0.000 claims description 4
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- 239000007787 solid Substances 0.000 description 25
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 18
- 240000001462 Pleurotus ostreatus Species 0.000 description 12
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Abstract
The invention discloses a rapid seed production method for edible fungi, and belongs to the technical field of edible fungus strain production. The method comprises the following steps of: 10-20, adding the edible fungus mother seeds into sterile water, homogenizing, then uniformly inoculating the edible fungus mother seeds on a PDA culture medium or a potato whole powder culture medium, and culturing until the edible fungus mother seeds grow to be full of the culture medium; scraping the mother seeds on the culture medium, adding the mother seeds into a liquid culture medium for detection, and observing whether the mother seeds are turbid or have peculiar smell after culturing for 2 days; inoculating the clarified and odorless mother strain liquid to the culture medium, mixing, and culturing until the culture bag is full of the mother strain liquid. The invention also discloses a method for detecting the purity of the edible fungus mother strain. The seed production method of the invention avoids the risk of pollution of the source of the strain through the purity detection step of the mother strain. Secondly, the method has fast strain production speed and high consistency of the strain age. In addition, the seed production method of the invention has simple required equipment and low production cost, and is suitable for small and medium-sized enterprises and individuals to produce strains.
Description
Technical Field
The invention belongs to the technical field of edible fungus strain production, and particularly relates to a method for rapid edible fungus seed production.
Background
In the production of edible fungi, the quality of strains directly determines the success or failure of production, so the strain production is the most critical first step in the production process of the edible fungi. At present, 2 kinds of edible fungus strains are mainly used in production, and one kind of edible fungus strain is a solid strain used in traditional agricultural cultivation. According to the edible fungus strain management method (issued by Ministry of agriculture on 6.1.2006), the edible fungus strain production implements three-stage breeding procedures of mother strain, stock strain and cultivated strain. According to the general technical requirements of edible fungus strains (industry standard NY/T1742-2009), the longest time required for the mother seeds to grow on a 90mm flat plate is 14-16 days of shiitake mushroom, 7-9 days of oyster mushroom, 14-16 days of black fungus, 10-14 days of needle mushroom, 28-32 days of agaricus bisporus and 14-16 days of auricularia polytricha; the longest time for the stock seeds to grow in the bottle is 50 days for the mushrooms, 30 days for the oyster mushrooms, 45 days for the black fungus, 35 days for the needle mushrooms, 45 days for the agaricus bisporus and 35 days for the auricularia polytricha; the longest time for cultivating seeds to grow full of bottles is 45 days for shiitake mushroom, 25 days for oyster mushroom, 40 days for black fungus, 30 days for needle mushroom, 40 days for agaricus bisporus and 30 days for auricularia polytricha. Therefore, the time for producing the seeds of the 6 kinds of edible fungi is about 110 days of mushroom, 62 days of oyster mushroom, 99 days of black fungus, 75 days of needle mushroom, 113 days of agaricus bisporus and 79 days of auricularia polytricha. Therefore, the solid strains used in the traditional agricultural cultivation of edible fungi have the defects of long period, multiple production links, complex operation, low growth efficiency, high cost, easy strain pollution and the like, and production accidents caused by strain pollution (especially mother strain pollution) often occur after the cultivation bags are inoculated in a large scale (strain pollution discovery is delayed), so that the loss is very large. The other is liquid spawn for industrial production of edible fungi. The liquid spawn is produced through submerged liquid culture, and has the advantages of fast spawn production, high yield, fast moisture transferring, high activity, high purity, less pollution, short culture period, low cost, etc. and is suitable for standardized, industrial and annual production. However, the liquid strain preparation has the defects of high investment on hardware equipment, construction of a sterile inoculation room, high technical requirements, need of hiring professional technicians and the like; for small factories or farmers who do strains, the difficulty of using the fermentation tank to produce the strains is great. Therefore, no one basically adopts liquid strains in agricultural production of edible fungi.
From the above, it is necessary to solve the problems of long production cycle and delayed bacterial contamination discovery in the conventional solid bacterial strains used in agricultural cultivation.
Disclosure of Invention
Aiming at the defects of long production period, low growth efficiency, delayed bacterial pollution discovery and the like existing in the traditional edible fungus solid strain production, the invention aims to provide a rapid edible fungus seed production method.
The invention also aims to provide a propagation method of the edible fungus mother strain.
The third purpose of the invention is to provide a method for detecting the purity of the edible fungus mother strain.
The fourth object of the present invention is to provide a liquid medium for detection.
The fifth purpose of the invention is to provide the application of the liquid culture medium for detection in edible fungus strain purity detection.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a method for rapidly producing seeds of edible fungi, which comprises the following steps:
(1) and (3) breeding mother seeds: edible fungus mother seeds are mixed according to the weight ratio of 1: adding 10-20 parts of the mixture into sterile water, and homogenizing to obtain a homogenate bacterial liquid; uniformly distributing the homogenate bacterial liquid on a PDA plate culture medium or a potato whole powder culture medium; culturing at proper temperature in dark for 3-7 days until the surface grows full;
(2) and detecting the purity: scraping the mother seed mycelium overgrown on the PDA plate culture medium or the potato whole powder culture medium in the step (1), placing the mother seed mycelium in a liquid culture medium for detection according to the weight ratio of the mother seed mycelium to the liquid culture medium for detection of 1: 5-10, and homogenizing; then, standing and culturing for 2d at the temperature of 25-28 ℃ under the condition of keeping out of the sun; observing whether the liquid culture medium for detection is turbid or not and whether peculiar smell exists or not; selecting and reserving mother strain liquid without turbidity and peculiar smell;
(3) diluting mother seeds, inoculating a culture medium of cultivated species: subpackaging the mother strain liquid without turbidity and peculiar smell obtained in the step (2) into sterile triangular flasks according to the weight ratio of 1: diluting the mother strain liquid with sterile water in a ratio of 10-15; inoculating the diluted mother strain liquid to a culture medium according to the weight ratio of 5-8%; when in inoculation, firstly taking off a strain cover outside a culture medium of a cultivated species, pulling out a plastic rod in the center of the culture medium of the cultivated species to expose a central hole, pouring mother strain liquid into the central hole, covering the strain cover, flatly placing a strain bag to allow the strain liquid to permeate to the wall of the culture medium, and when in placement, turning over the strain bag to enable one surface with the strain liquid to face upwards and the strain liquid to permeate to the other surface of the culture medium, thereby achieving the effect of mixing the culture medium and the strain liquid; culturing at proper temperature in dark until the culture medium is full of mycelia to obtain the culture strain of the edible fungi.
The suitable temperature in the step (1) of the seed production method is 23-29 ℃, the corresponding optimum temperature is selected according to different edible fungi, and the specific temperature is carried out according to the standard in table 3 of the general technical requirements of edible fungi strains (industry standard NY/T1742-.
The PDA plate medium described in the above-mentioned step (1) of the preparation method is prepared according to a conventional method.
The potato whole powder culture medium in the step (1) of the preparation method comprises the following components in percentage by weight: 10-20 g of potato powder, 2-10 g of glucose, 12-20 g of agar powder and 1000ml of water.
The preparation method of the potato whole powder culture medium comprises the following steps: weighing potato powder, glucose and agar powder according to weight ratio, mixing well, adding into container (such as triangular flask), adding water proportionally, shaking well, sealing with sealing film or kraft paper, and sterilizing at 121 deg.C for 30 min. Wherein potato flakes, dextrose and agar powder are commercially available.
The seed production method comprises the following components in the step (2) of the detection liquid culture medium: 20g of glucose, 2g of yeast powder and 1000ml of water.
The preparation method of the liquid culture medium for detection comprises the following steps: adding glucose and yeast powder into water at a certain proportion, mixing well, and sterilizing at 121 deg.C for 30 min.
The raw materials of the culture medium of the cultivated species in the step (3) of the seed production method are wheat grains, wood chips, cotton seed hulls or corn cobs and the like; selecting different culture mediums according to edible strains.
The moderate temperature in the step (3) of the seed production method is 20-31 ℃; different edible fungi select different culture temperatures. The specific temperature is according to the temperature specified in table 6 in the general technical requirements of edible fungus strains (industry standard NY/T1742-2009).
The invention also provides a propagation method of the edible fungus mother seeds, which comprises the following steps of: adding 10-20 parts of the mixture into sterile water, and homogenizing to obtain a homogenate bacterial liquid; uniformly distributing the homogenate bacterial liquid on a PDA plate culture medium or a potato whole powder culture medium; culturing at suitable temperature and in dark condition for 3-7 days until the surface grows full.
The suitable temperature in the propagation method is 23-29 ℃, the corresponding optimum temperature is selected according to different edible fungi, and the specific temperature can be determined according to the standard in table 3 of the edible fungi species general technical requirements (industry standard NY/T1742-2009).
The invention also provides a method for detecting the purity of the edible fungus mother strain, which comprises the following steps:
(1) preparing a liquid culture medium for detection according to the proportion that each 1000ml of water contains 20g of glucose and 2g of yeast powder;
(2) scraping the edible fungus mother seeds on the PDA plate culture medium or the potato whole powder culture medium, adding the edible fungus mother seeds into the liquid culture medium for detection prepared in the step (1) according to the weight ratio of 1: 5-10, and homogenizing; then, carrying out static culture on the liquid culture medium for detection for 2d at the temperature of 25-28 ℃ under the condition of keeping out of the sun;
(3) observing whether the culture medium is turbid or not and whether peculiar smell exists or not; the edible fungus mother strain without turbidity and peculiar smell is pollution-free and high in purity.
The invention also provides a liquid culture medium for detection, which comprises the following components in percentage by weight: 20g of glucose, 2g of yeast powder and 1000ml of water.
The liquid culture medium for detection is applied to purity detection of edible fungus strains.
The invention has the advantages and beneficial effects that: (1) in the seed production method, the mother seeds are subjected to a purity detection step, so that the risk of source pollution of the strains is avoided. (2) The method has the advantages of high strain production speed and high strain age consistency. (a) Compared with the conventional method that hyphae spread around the culture medium after germination at the central inoculation point, the method ensures that the inoculation points are distributed on the surface of the culture medium completely, the hyphae grow over the flat plate about 1 day after germination, and the full time is only about half of that required by the conventional method. (b) The mother seeds are directly inoculated to the culture medium of the cultivated species, the original seed production link is saved, and the time for producing the strains is greatly shortened, for example, the time for producing the oyster mushroom is shortened to 13-16 days from the conventional about 60 days; the required time of the mushrooms is shortened from about 110 days to 24-26 days. (c) In the conventional seed production, the hypha grows from the upper surface to the bottom, the required time is long, and the fungus ages of the surface and the bottom are different greatly, for example, the difference between oyster mushroom and shiitake mushroom is about 20 days, and the difference between shiitake mushroom and oyster mushroom is about 40 days. The invention adopts multipoint germination, and the parts of the bacteria liquid contacting the culture material are all germination points, so the consistency of the age of the bacteria is very high, the difference between the oyster mushrooms is about 3 days, and the difference between the mushrooms is about 7 days. The fungus age consistency is high, the fungus growing speed on the cultivation bags is approximately the same, and the cultivation bags are suitable for fruiting management. (3) The seed production method of the invention has simple required equipment and low production cost. The traditional seed production mode has the original seed production link, so the culture time is long, the turnover rate of a culture room is low, and the energy consumption is high. However, the use of a fermenter for liquid culture requires the purchase of the fermenter, the construction of a sterile inoculation room, the employment of fermentation technicians, and the like, and therefore, the cost is high. The invention can achieve the effect of making liquid strains in the fermentation tank only by the superclean workbench, the triangular flask and the homogenizer, and is flexible and mobile. The invention is suitable for small and medium-sized enterprises and individuals to produce strains.
Description of the drawings:
FIG. 1 is a photograph of a bacterial liquid not contaminated by bacteria in the case of mother strain purity measurement.
FIG. 2 is a photograph of bacterial liquid contaminated by bacteria during purity detection of mother strains.
FIG. 3 is a photograph of mycelia of Pleurotus Ostreatus cultured for 6 days after inoculation with the mother strain liquid of the present invention.
FIG. 4 is a photograph of mycelia of Pleurotus Ostreatus cultured for 6 days after inoculation with solid strains.
FIG. 5 is a photograph of mycelia of needle mushroom cultured for 20 days after inoculation with the mother strain liquid of the present invention.
FIG. 6 is a photograph of mycelia of needle mushroom cultured for 20 days after inoculation with solid seed culture.
FIG. 7 is a photograph of 25-day hyphae of mushroom inoculated with the mother strain liquid of the present invention.
FIG. 8 is a photograph of mycelia cultured for 25 days after inoculation of Lentinus edodes with solid strains.
FIG. 9 is a photograph of hyphae of Auricularia auricula (L.) Underw inoculated with the mother strain liquid of the present invention and cultured for 25 days.
FIG. 10 is a photograph of mycelia of Auricularia auricula (L.) Underw inoculated with solid strain and cultured for 25 days.
FIG. 11 is a photograph of mycelia of P.nebrodensis cultured for 18 days after inoculation with the mother strain liquid of the present invention.
FIG. 12 is a photograph of mycelia of P.nebrodensis cultured for 18 days after inoculation with solid strains.
FIG. 13 is a photograph of mycelia of Pleurotus eryngii cultured for 18 days after inoculation with the mother strain solution of the present invention.
FIG. 14 is a photograph of mycelia of Pleurotus eryngii cultured for 18 days after inoculation with solid strains.
The specific implementation mode is as follows:
the present invention is further illustrated by the following examples, which are not intended to limit the scope of the invention in any way.
Example 1 contrast test for Pleurotus ostreatus strain preparation
The method comprises the following steps:
(1) the rapid propagation of the mother seeds is carried out under the aseptic condition according to the weight ratio of 1: 15, adding the mother seeds into sterile water according to a proportion, and homogenizing to obtain a homogenate bacterial liquid; uniformly distributing the homogenate bacterial liquid on the surface of the PDA plate culture medium by using a sterile spray can or a coater, sealing the edge of a culture dish, culturing for 3-4 days at 25 ℃ under the condition of keeping out of the sun, and growing hyphae on the plate to obtain the mother strain.
(2) Mother seed purity detection 100ml of liquid culture medium (the components of which are 20g of glucose, 2g of yeast powder and 1000ml of water) for detection is filled into a 250ml triangular flask, sterilized at 121 ℃ for 30min, and cooled to normal temperature for standby. Scraping all the culture medium on the full PDA plate culture medium in the step (1) to 1 triangular flask corresponding to 1 plate, and homogenizing under aseptic condition; standing and culturing at 25 deg.C in dark condition for 2d to obtain mother strain liquid; and observing whether the culture medium is turbid or not and whether peculiar smell exists or not. The culture medium is clear (see figure 1), has no peculiar smell, indicates that the mother seeds are pure, and can be subjected to next step propagation; otherwise, the medium was turbid (see FIG. 2), and the mother seed was contaminated and could not be used.
(3) Diluting the mother seeds, inoculating a culture medium of the cultivated species: and (3) mixing the clarified and odorless mother strain liquid according to the ratio of 1: 10, adding sterile water for dilution; soaking the sterilized wheat grain culture medium (comprising 98% of wheat grains and 2% of gypsum, see NY/T528-2010 edible fungus strain production technical specification appendix B.5. the wheat grains are soaked in clear water for 24-36h, fishing out and draining, adding gypsum powder in proportion, uniformly mixing, bagging, sterilizing at 126 ℃ for 2h, cooling to room temperature), inoculating the diluted mother strain liquid according to the inoculation amount of 5% by weight, and uniformly shaking. Placing into a culture chamber, culturing at 25 deg.C in dark for 8-10 days until the culture bag is full.
Meanwhile, conventional solid strains are prepared by a traditional method, wheat grains are respectively inoculated (the formula is the same, a liquid culture medium is directly poured from a strain mouth or a solid culture medium is inoculated to the surface of the wheat grains), and the wheat grains are cultured under the same condition.
As a result, the mother strain liquid prepared by the method is inoculated and cultured for 6 days (see figure 3), hyphae are close to and fully distributed on wheat grains, and all the hyphae fully grow after about 9 days of culture; when cultured for 6 days after inoculation with conventional solid strains (see FIG. 4), hyphae just germinated a little and finally grew full for 23 days.
From the results, compared with the traditional solid strains, the method for preparing the pleurotus ostreatus mother strain liquid for inoculating the wheat seeds has the advantage that the seed production time is shortened by 60 percent. The method has the advantages of short edible fungus seed production period, high seed production speed and avoidance of the problem of mother seed pollution.
Example 2 comparison test for production of Flammulina velutipes, Lentinus edodes, and Auricularia auricula strains
The method comprises the following steps:
(1) the rapid propagation of the mother seeds is carried out under the aseptic condition according to the weight ratio of 1: 10, adding needle mushroom, mushroom or black fungus mother seeds into sterile water, and homogenizing to obtain a homogenate bacterial liquid; and uniformly distributing the homogenate bacterial liquid on the surface of the PDA culture medium by adopting a sterile spray can or a coater, sealing the edge of a culture dish, and culturing for 6-7 days at 25 ℃ in a dark place, wherein hyphae grow over the flat plate, thus obtaining the mother strain.
(2) The method for detecting the purity of the mother seeds is the same as the step (2) of the example 1.
(3) Diluting the mother seeds, inoculating a culture medium of the cultivated species, and mixing the clarified and odorless mother seed bacterial liquid obtained in the step (2) according to the weight ratio of 1: 10 diluted with sterile water. Weighing sawdust, bran and gypsum in proportion in a sterilized sawdust culture medium (the components of which are 78% of sawdust, 20% of bran, 1% of sucrose and 1% of gypsum, see NY/T528-. Placing the mixture into a culture room, culturing for 22-28 days at 25 ℃ under the condition of keeping out of the sun, and growing the strain bags.
Meanwhile, conventional solid strains are respectively inoculated with a wood chip culture medium (the formula is the same, a central hollow plastic rod is pulled out, a liquid culture medium is directly poured from a strain port to the center, or a solid culture medium is inoculated to the surface of the wood chip culture medium), and the culture is carried out under the same condition.
As a result, the flammulina velutipes is inoculated by the mother strain liquid prepared by the method and cultured for 20 days (see figure 5), and hyphae overgrow with a wood chip culture medium; when the solid seed culture was used for inoculation and cultured for 20 days (see FIG. 6), the hyphae were overgrown with about 4/5.
The mushroom is inoculated by the mother strain liquid prepared by the method and cultured for 25 days (see figure 7), and hyphae nearly overgrow a wood chip culture medium; when cultured for 25 days after inoculation with conventional solid strains (see FIG. 8), the hyphae overgrow with about 4/5.
The black fungus is inoculated by the mother strain liquid prepared by the method and cultured for 25 days (see figure 9), and hyphae nearly overgrow a wood chip culture medium; when cultured for 25 days after inoculation with conventional solid strains (see FIG. 10), the hyphae overgrow with about 1/2.
As can be seen from the results of the comparative tests, compared with the traditional solid strains, the seed production time is shortened by more than 10 days by inoculating the wood chip culture medium with the mother strain liquid of the flammulina velutipes, the lentinus edodes and the black fungus prepared by the method. The method for producing the seeds has short period and high speed, and can avoid the pollution of the mother seeds.
Example 3 Pleurotus nebrodensis and Pleurotus eryngii species preparation comparison test
The method comprises the following steps:
(1) the rapid propagation of the mother seeds is carried out under the aseptic condition according to the weight ratio of 1: 10, adding the pleurotus nebrodensis or pleurotus eryngii mother strains into sterile water, and homogenizing to obtain homogenate bacterial liquid; and uniformly distributing the homogenate bacterial liquid on the surface of the PDA culture medium by adopting a sterile spray can or a coater, sealing the edge of a culture dish, culturing for 4-5 days at 25 ℃ under the condition of keeping out of the sun, and growing hyphae on the flat plate to obtain the mother strain.
(2) The mother seed purity was determined in the same manner as in step (2) of example 1.
(3) Diluting the mother seeds, inoculating a culture medium of the cultivated species, and mixing the clarified and odorless mother seed bacterial liquid obtained in the step (2) according to the weight ratio of 1: 10, adding sterile water for dilution. Inoculating the diluted mother strain liquid to a sterilized cottonseed hull culture medium (the components of the sterilized cottonseed hull culture medium are 99% of cottonseed hulls and 1% of lime, see NY/T528-2010 edible fungus strain production technical specification appendix B.3.1. weighing the cottonseed hulls and the lime in proportion, adding water into the culture medium according to the weight ratio of the culture medium to the water of 2: 3, uniformly mixing to ensure that the water is fully absorbed, bagging, inserting a hollow plastic rod into the center, sealing without a cotton cover body, sterilizing at 126 ℃ for 2h, cooling to room temperature) according to the proportion of 5% by weight, and uniformly shaking; placing the mixture into a culture room, culturing for 20-23 days at 25 ℃ under the condition of keeping out of the sun, and growing the strain bags.
Meanwhile, conventional solid strains are respectively inoculated to cottonseed hull culture mediums (the formula is the same, a central hollow plastic rod is pulled out, a liquid culture medium is directly poured from a strain port to the center, or a solid culture medium is inoculated to the surface of the cottonseed hull culture medium), and the culture is carried out under the same conditions.
As a result, after the pleurotus nebrodensis is cultured for 18 days by using the mother strain liquid prepared by the method of the invention (see figure 11), the hyphae overgrow with about 2/3 of the cottonseed hull culture medium; after culturing for 16 days using conventional solid strains (see FIG. 12), the hyphae overgrow with about 1/3.
The pleurotus eryngii is inoculated by the mother strain liquid prepared by the method and cultured for 18 days (see figure 13), and hyphae grows over about 4/5 of the cottonseed hull culture medium; when the culture was carried out for 18 days after inoculation with the solid seed culture (see FIG. 14), the mycelia grew to about 1/3.
From the results, compared with the traditional solid strains, the seed production time is shortened by 5-10 days by inoculating the mother strain liquid of the pleurotus nebrodensis and the pleurotus eryngii prepared by the method to the cottonseed hull culture medium. The method has short seed production period and high seed production speed, and avoids the problem of mother seed pollution.
In addition, the results also show that the seed production method is simple, the required equipment is simple, and the manufacturing cost is low.
Claims (3)
1. A method for rapidly producing seeds of edible fungi is characterized by comprising the following steps:
(1) and (3) mixing the edible fungus mother seeds in a weight ratio of 1: adding 10-20 parts of the mixture into sterile water, and homogenizing to obtain a homogenate bacterial liquid; uniformly distributing the homogenate bacterial liquid on a potato whole powder culture medium; culturing at proper temperature in dark for 3-7 days until the surface grows full;
(2) scraping the mother seed mycelium overgrowing the whole potato powder culture medium in the step (1), placing the mother seed mycelium in a liquid culture medium for detection according to the weight ratio of the mother seed mycelium to the liquid culture medium for detection of 1: 5-10, and homogenizing; then, standing and culturing for 2d at the temperature of 25-28 ℃ under the condition of keeping out of the sun; observing whether the liquid culture medium for detection is turbid or not and whether peculiar smell exists or not; selecting and reserving mother strain liquid without turbidity and peculiar smell; wherein the liquid culture medium for detection comprises the following components in percentage by weight: 20g of glucose, 2g of yeast powder and 1000ml of water;
(3) subpackaging the mother strain liquid without turbidity and peculiar smell obtained in the step (2) into sterile triangular flasks according to the weight ratio of 1: diluting the mother strain liquid with sterile water in a ratio of 10-15; inoculating the diluted mother strain liquid to a culture medium according to the weight ratio of 5-8%; when in inoculation, firstly taking off a strain cover outside a culture medium of a cultivated species, pulling out a plastic rod in the center of the culture medium of the cultivated species to expose a central hole, pouring mother strain liquid into the central hole, covering the strain cover, flatly placing a strain bag to allow the strain liquid to permeate to the wall of the culture medium, and when in placement, turning over the strain bag to enable one surface with the strain liquid to face upwards and the strain liquid to permeate to the other surface of the culture medium, thereby achieving the effect of mixing the culture medium and the strain liquid; culturing at proper temperature in dark until the culture medium is full of mycelia to obtain the culture strain of the edible fungi;
the potato whole powder culture medium comprises the following components in percentage by weight: 10-20 g of potato powder, 2-10 g of glucose, 12-20 g of agar powder and 1000ml of water.
2. The method for producing seeds according to claim 1, characterized in that the suitable temperature in step (1) is 23-29 ℃.
3. The method for producing seeds according to claim 1, characterized in that the suitable temperature in the step (3) is 20-31 ℃.
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