CN106676018B - Agaricus bisporus strain breeding method suitable for standardized factory - Google Patents
Agaricus bisporus strain breeding method suitable for standardized factory Download PDFInfo
- Publication number
- CN106676018B CN106676018B CN201611264852.7A CN201611264852A CN106676018B CN 106676018 B CN106676018 B CN 106676018B CN 201611264852 A CN201611264852 A CN 201611264852A CN 106676018 B CN106676018 B CN 106676018B
- Authority
- CN
- China
- Prior art keywords
- sterilization
- strain
- agaricus bisporus
- culture
- triticale
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention discloses an agaricus bisporus strain breeding method suitable for a standardized chemical plant, which comprises the steps of selecting agaricus bisporus strain as a mother strain, breeding, fruiting experiment, stock preparation, selecting triticale as a raw material, soaking the raw material with the pH value of 11.0-13.0, stirring the raw material with the pH value of 7.0-9.0, quantitatively packaging a half bag by a sterilization bag, sealing the half bag by a lantern ring and a breathing plug, sterilizing at the high temperature of 120-130 ℃, 0.10-0.20 MPa and 2-3 h, cooling, performing aseptic inoculation, and culturing and cultivating the agaricus bisporus strain at constant temperature; the method can breed high-quality pure and high-yield agaricus bisporus cultivated species in large batch, greatly shortens the cultivation period, reduces the pollution probability, has the general cultivation time of 15-18 days, ensures that the cultivated strain grains have consistent water content, does not grow the mushroom quilt, has the qualification rate of 96 percent, and has wide practicability in the field of strain breeding.
Description
Technical Field
The invention belongs to the technical field of edible mushroom cultivation technology, and particularly relates to the technical field of an agaricus bisporus strain breeding method suitable for a standardized factory.
Background
Edible fungi are future health food for human beings, and artificial cultivation of edible fungi has become an emerging modern agricultural branch. In the aspect of agricultural ecological cycle, the cultivation of edible fungi occupies an important position. At present, the industrialization of edible fungi is vigorously developing all over the country. The main variety of the edible fungi is straw rotting fungi Agaricus bisporus (Agaricus bisporus), has high protein content, is fresh, tender and delicious, is popular with people all over the world, occupies 80 percent of artificial cultivation specific gravity in the aspect of artificial cultivation of the edible fungi, and is a main product of the edible fungi.
The conventional mode of cultivating the agaricus bisporus by the artificial soil method is in transition to the mode of producing the agaricus bisporus by semi-factory and factory, the standard agaricus bisporus factory is rising like bamboo shoots in spring after raining, however, the put-in agaricus bisporus factory has high yield, general quality and high pest and disease incidence rate, economic benefit or loss or imminent loss and is considered as the reason, and the core problem affecting the agaricus bisporus industry in China is that the technical process is backward in agaricus bisporus strain cultivation in China, no standard agaricus bisporus strain factory exists, the quality of strains for production is poor and the properties are unstable, in the agaricus bisporus cultivation enterprises, most agaricus bisporus cultivation enterprises still use the strains of the traditional workshop type strain cultivation factory except that expensive strains are introduced from abroad, the majority of agaricus bisporus cultivation enterprises use glass bottles of 500M L, after mother seeds are inoculated into the containers, only the strains can grow from the upper ends of the agaricus bisporus strains to the lower ends of the traditional workshop type strain cultivation bottles, the mushroom strains grow slowly and grow in the mycelium bottles, the mycelium is not uniform, the mycelium is not inoculated into the environment-resistant mycelium growth bottle, the mycelium is not polluted, the mycelium growth rate is not uniform, the humidity is not serious, the humidity is not required, the mycelia, the mycelium growth of the mycelium is not polluted, the fungi, the mycelium growth of the fungi is not polluted, the fungi is not uniform, the fungi is not polluted by the fungi, the fungi is not needed, the fungi is not serious disease-resistant fungi, the fungi is not serious, the fungi is not aged fungi, the fungi is not serious, the fungi is not aged fungi, the fungi growing soil is not aged fungi, the fungi is.
Disclosure of Invention
Aiming at the defects and problems of the current state of the prior art, in particular to the bottleneck problems that the quality of the traditional workshop type agaricus bisporus strain is low, the character is unstable and the development of the agaricus bisporus industry in China is influenced, in order to solve the problems, the invention provides the agaricus bisporus strain breeding method suitable for the standardized factory, compared with the prior art, the method can breed high-quality and pure and high-yield agaricus bisporus cultivated species in large batch, the cultivation period is greatly shortened compared with the traditional cultivation method by 30-45 days, the pollution probability is reduced, the general cultivation time of the cultivated species is 15-18 days, the black wheat is not required to be cooked, the energy is saved, the water content of the cultivated strain grains is consistent, the phenomenon of long fungus quilt is not generated, the qualification rate can reach 96 percent, and no matter the grains are used as solid strains for the third-level species breeding, liquid strains are used and can be uniformly mixed or sprayed into the triticale culture medium in a ten-grade purification level environment, the growth points are many, the germination is fast, the inoculation time on each particle of the same batch of matrix is basically consistent, so the initial growth time points are consistent, the fungus ages are short, the strain viability is strong, the hypha distribution is uniform and standard, and the method has wide practicability in the field of strain cultivation.
The invention provides a agaricus bisporus strain breeding method suitable for a standardized factory, which specifically adopts the following technical steps:
(1) preparing a stock: selecting high-quality and vigorous agaricus bisporus strain as mother strain, breeding, and performing fruiting experiment, and has high fruiting rate, good quality and yield of 30Kg/m2The above strains are used as parent strains of agaricus bisporus strains; the agaricus bisporus strain mother seeds are cultivated into agaricus bisporus strain solid original seeds by taking millet as a hypha carrier through original seed cultivation or prepared nutrient solution is cultivated into agaricus bisporus strain liquid original seeds by a liquid strain fermentation tank through original seed cultivation.
(2) Selecting raw materials: screening the triticale, removing broken grains, shrunken grains and impurities, and selecting the triticale which is clean, full, complete, fresh and mildew-free.
(3) Soaking raw materials: and (3) putting water into the soaking pool, dissolving quicklime to enable the pH value to reach 11.0-13.0, uniformly mixing, putting a soaking cage into the soaking pool, putting triticale into the soaking cage, stirring with compressed air for 4-6 times a day, and uniformly absorbing water by the triticale.
(4) Stirring raw materials: and testing that the water content of the triticale reaches the standard, lifting out the soaking cage by using a crane, controlling water until no obvious water drops exist, pouring the soaking cage into a stirrer, adding 1% of calcium carbonate and 0.8% of calcium sulfate by mass, uniformly stirring, adjusting the pH value to 7.0-9.0, and completing stirring.
(5) And (4) quantitative packaging: introducing the stirred triticale into a conveyor belt, feeding into an automatic quantitative packaging machine, quantitatively packaging half bags with a sterilization bag, sealing with a lantern ring and a breathing plug, and placing on a sterilization trolley, wherein each layer comprises 6 bags, and the number of the layers is 9.
(6) High-temperature sterilization: after the sterilization cart is filled, opening sterilization cabinet doors of a bacteria-containing area, pushing the sterilization cabinets into the sterilization cabinets, arranging the sterilization cabinets in sequence, sealing the sterilization cabinets, vacuumizing, introducing steam for sterilization, wherein the sterilization parameters are 120-130 ℃, 0.10-0.20 MPa and 2-3 h, after the sterilization is finished, exhausting and reducing pressure, when the pressure reaches zero, opening the sterilization cabinet doors of a hundred-grade purification area on the side of a purification chamber, pulling out the sterilization cart, and cooling the sterilization cart in the hundred-grade purification cooling area for 10-14 h to ensure that the material temperature reaches 18-25 ℃.
(7) And (3) sterile inoculation: transferring the cooled culture material to a ten-stage purification area, opening a sterilization bag breather plug by an inoculating person on an operation table, inoculating a solid stock strain of the agaricus bisporus strain or a liquid stock strain of the agaricus bisporus strain to a sterilization bag according to 1 percent of inoculation amount, shaking up, pouring two sterilization bags into one culture bag, sealing the culture bag with a breather membrane by a sealing machine, and transferring the culture bag out of a culture room for culture.
(8) Constant-temperature culture: the temperature of the ten thousand-level purification culture room is set to be constant at 24 ℃, the culture is carried out for 15 to 18 days, the strains are mature, and the strains are transmitted to a constant-temperature cold storage warehouse at 2 ℃ for storage.
In the invention, the pH value of the material soaking pool in the material soaking step is preferably 12.0, the pH value of the material stirring pool in the material soaking step is preferably 8.0, the material temperature is 21.5 ℃, and the sterilization parameters in the high-temperature sterilization step are preferably 125 ℃, 0.15MPa and 2.5 h.
The agaricus bisporus strain, millet, nutrient solution, a liquid strain fermentation tank, triticale, quicklime, a soaking cage, a boat crane, a stirrer, calcium carbonate, calcium sulfate, an automatic quantitative packing device, a sterilization bag, a lantern ring, a breather plug, a sterilization cart, a sterilization cabinet and a culture bag which are adopted by the agaricus bisporus strain automatic quantitative packing device can be purchased or customized through public channels.
The technical scheme provided by the invention can achieve the following beneficial effects:
(1) according to the agaricus bisporus strain breeding method suitable for the standardized factory, provided by the invention, through orthogonal test and a response surface method optimization process, high-quality and pure and high-yield agaricus bisporus cultivated species can be bred in a large batch, the cultivation period is greatly shortened compared with the conventional cultivation method that the cultivation period is 30-45 days, the pollution probability is reduced, the general cultivation time of the cultivated species is 15-18 days, the black wheat does not need to be cooked, the energy is saved, the water content of cultivated strain grains is consistent, the phenomenon of fungus growth does not occur, and the qualification rate can reach 96%.
(2) The agaricus bisporus strain breeding method suitable for the standardized factory, provided by the invention, has the advantages that as the second-level strain is selected as the breeding third-level strain, no matter grains are used as solid strains or liquid strains are used, the second-level strain and the liquid strains can be uniformly mixed or sprayed into a triticale culture medium in a ten-level purification environment, the growth points are many, the germination is fast, and the inoculation time on each grain of the same batch of matrix is basically consistent, so that the initial growth time points are consistent, the fungus age is short, the vitality of the strains is strong, the hypha distribution is uniform and standard, and the agaricus bisporus strain breeding method has wide practicability in the field of strain breeding.
Drawings
FIG. 1 shows a process flow diagram provided for the present invention.
Fig. 2 shows a graph of response surface of sterilization temperature and sterilization pressure versus yield provided by the present invention.
Fig. 3 shows a graph of response surface of sterilization temperature and sterilization time versus yield provided by the present invention.
Fig. 4 shows a graph of the response of sterilization pressure and sterilization time to yield provided by the present invention.
Detailed Description
The following description will be given in further detail with reference to the accompanying fig. 1 to 4 and examples, but the present invention is not limited to the following examples.
In the present invention, for convenience of description, in the agaricus bisporus strain breeding method applied to a standardized factory provided by the present invention, the description of the relative position relationship of the components is described according to the layout mode of the attached fig. 1, such as: the positional relationship of up, down, left, right, etc. is determined in accordance with the layout direction of fig. 1.
The agaricus bisporus strain, millet, nutrient solution, a liquid strain fermentation tank, triticale, quicklime, a soaking cage, a boat crane, a stirrer, calcium carbonate, calcium sulfate, an automatic quantitative packing device, a sterilization bag, a lantern ring, a breather plug, a sterilization cart, a sterilization cabinet and a culture bag which are adopted by the agaricus bisporus strain automatic quantitative packing device can be purchased or customized through public channels.
All materials, reagents and equipment selected for use in the present invention are well known in the art, but do not limit the practice of the invention, and other reagents and equipment well known in the art may be suitable for use in the practice of the following embodiments of the invention.
The first embodiment is as follows: agaricus bisporus strain breeding method suitable for standardized factory
The invention provides a agaricus bisporus strain breeding method suitable for a standardized factory, which specifically adopts the following technical steps:
(1) preparing a stock: selecting high-quality and vigorous agaricus bisporus strain as mother strain, breeding, and performing fruiting experiment, and has high fruiting rate, good quality and yield of 30Kg/m2The above strains are used as parent strains of agaricus bisporus strains; the agaricus bisporus strain mother seeds are cultivated into agaricus bisporus strain solid original seeds by taking millet as a hypha carrier through original seed cultivation or prepared nutrient solution is cultivated into agaricus bisporus strain liquid original seeds by a liquid strain fermentation tank through original seed cultivation.
(2) Selecting raw materials: screening the triticale, removing broken grains, shrunken grains and impurities, and selecting the triticale which is clean, full, complete, fresh and mildew-free. Compared with the traditional common wheat for planting, the triticale mycelium carrier has thick and tough seed coat, is not easy to break after being soaked and expanded so as to overflow out starch, can keep complete shape after being autoclaved and has water content of more than 50 percent.
(3) Soaking raw materials: and (3) putting water into the soaking pool, dissolving quicklime to enable the pH value to reach 11.0-13.0, uniformly mixing, putting a soaking cage into the soaking pool, putting triticale into the soaking cage, stirring with compressed air for 4-6 times a day, and uniformly absorbing water by the triticale. The auxiliary material calcium oxide is used for increasing the pH value of water, so that the wax coat of the triticale is damaged, and the triticale rapidly absorbs water and swells. Meanwhile, the method effectively stops the existence of external infectious microbes in the strong alkaline solution, and also effectively kills the infectious microbe spores hidden in the triticale, so that the carrying of the infectious microbes in the water absorption process of the triticale is reduced as much as possible. The rye soaked by the compressed air is stirred, so that the rye can be effectively mixed and loosened, the rye is fully and uniformly contacted with water during soaking, and the purpose that the rye contains sufficient and uniform water is achieved. Thereby ensuring the hypha to grow vigorously. The soaking process can not only enable the triticale to reach the optimal water content state, but also successfully solve the technical problems that the traditional process is easy to damage and explode when used for cooking the raw material wheat, can not ensure the integrity of the raw material and is easy to cause the pollution of mixed bacteria, and reduce or even stop the pollution in the subsequent process.
(4) Stirring raw materials: and testing that the water content of the triticale reaches the standard, lifting out the soaking cage by using a crane, controlling water until no obvious water drops exist, pouring the soaking cage into a stirrer, adding 1% of calcium carbonate and 0.8% of calcium sulfate by mass, uniformly stirring, adjusting the pH value to 7.0-9.0, and completing stirring. The addition of the auxiliary materials of calcium carbonate and calcium sulfate can effectively reduce the pH reduction situation of the raw materials during high-temperature sterilization and ensure the pH of the sterilized raw materials, and on the other hand, compared with the addition of the dried cow dung and the bran in the traditional strain production, the addition of the auxiliary materials of calcium carbonate and calcium sulfate can more effectively avoid the incubation of mixed bacteria and reduce the pollution probability.
(5) And (4) quantitative packaging: introducing the stirred triticale into a conveyor belt, feeding into an automatic quantitative packaging machine, quantitatively packaging half bags with a sterilization bag, sealing with a lantern ring and a breathing plug, and placing on a sterilization trolley, wherein each layer comprises 6 bags, and the number of the layers is 9. The sterilization bag is used for quantitatively packaging the half bags, so that the optimal sterilization effect can be achieved, and the optimal shaking effect can be achieved in the bag shaking stage during inoculation; the bag mouth is sealed by a lantern ring and a breathing plug, so that the problem of bag explosion in the sterilization cabinet is solved, and meanwhile, aseptic cooling in a hundred-grade cooling stage is further ensured.
(6) High-temperature sterilization: after the sterilization cart is filled, opening sterilization cabinet doors of a bacteria-containing area, pushing the sterilization cabinets into the sterilization cabinets, arranging the sterilization cabinets in sequence, sealing the sterilization cabinets, vacuumizing, introducing steam for sterilization, wherein the sterilization parameters are 120-130 ℃, 0.10-0.20 MPa and 2-3 h, after the sterilization is finished, exhausting and reducing pressure, when the pressure reaches zero, opening the sterilization cabinet doors of a hundred-grade purification area on the side of a purification chamber, pulling out the sterilization cart, and cooling the sterilization cart in the hundred-grade purification cooling area for 10-14 h to ensure that the material temperature reaches 18-25 ℃. The high-temperature sterilization step can thoroughly sterilize in the shortest time and under the condition of least energy consumption.
(7) And (3) sterile inoculation: transferring the cooled culture material to a ten-stage purification area, opening a sterilization bag breather plug by an inoculating person on an operation table, inoculating a solid stock strain of the agaricus bisporus strain or a liquid stock strain of the agaricus bisporus strain to a sterilization bag according to 1 percent of inoculation amount, shaking up, pouring two sterilization bags into one culture bag, sealing the culture bag with a breather membrane by a sealing machine, and transferring the culture bag out of a culture room for culture. The culture bag with the breathing membrane can effectively isolate the invasion of mixed bacteria in the culture stage, and ensure the pure culture of the strains.
(8) Constant-temperature culture: the temperature of the ten thousand-level purification culture room is set to be constant at 24 ℃, the culture is carried out for 15 to 18 days, the strains are mature, and the strains are transmitted to a constant-temperature cold storage warehouse at 2 ℃ for storage.
The process flow of the agaricus bisporus strain breeding method suitable for the standardized factory provided by the invention is shown in the attached figure 1.
Example two: agaricus bisporus strain breeding method suitable for standardized factory
Selecting high-quality and vigorous agaricus bisporus strain as mother strain, breeding, and performing fruiting experiment, and has high fruiting rate, good quality and yield of 30Kg/m2The above strains are used as parent strains of agaricus bisporus strains; adopting millet as a hypha carrier to cultivate the parent strain of the agaricus bisporus strain to prepare a solid parent strain of the agaricus bisporus strain or prepare nutrient solution to cultivate the parent strain in a liquid strain fermentation tank to prepare a liquid parent strain of the agaricus bisporus strain; screening the triticale, removing broken grains, shrunken grains and impurities, and selecting the triticale which is clean, full, complete, fresh and mildew-free; putting water into a soaking pool, dissolving quicklime to enable the pH value to reach 11.0, uniformly mixing, putting a soaking cage into the soaking pool, putting triticale into the soaking cage, stirring with compressed air, and uniformly absorbing water of the triticale 4 times a day; testing that the water content of the triticale reaches a standard, lifting out the soaking cage by using a crane, controlling water until no obvious water drops exist, pouring the soaking cage into a stirrer, adding 1% of calcium carbonate and 0.8% of calcium sulfate by mass, uniformly stirring, adjusting the pH value to 7.0, and completing stirring; introducing the stirred triticale into a conveyor belt, entering an automatic quantitative packaging machine, quantitatively packaging half bags by using a sterilization bag, sealing by using a lantern ring and a breathing plug, and putting on a sterilization trolley, wherein each layer of 6 bags is 9 layers; high-temperature sterilization: after the sterilization cart is filled, opening sterilization cabinet doors in a bacteria area, pushing the sterilization cabinets into the sterilization cabinets, arranging the sterilization cabinets in sequence, sealing the sterilization cabinets, vacuumizing, introducing steam for sterilization, wherein the sterilization parameters are 120 ℃, 0.10MPa and 2h, exhausting and reducing pressure, opening the sterilization cabinet doors in a hundred-grade purification area on the side of a purification chamber when the pressure reaches zero, pulling out the sterilization cart, and cooling the sterilization cart in a hundred-grade purification cooling area for 10h to ensure that the material temperature reaches 18 ℃; and (3) sterile inoculation: transferring the cooled culture material to ten-stage purification area, and opening sterilization bag by inoculating personnel on operation tableA breather plug, inoculating the solid stock of the agaricus bisporus strain or the liquid stock of the agaricus bisporus strain into a sterilization bag according to 1 percent of inoculation amount, shaking up, pouring the two sterilization bags into one culture bag, sealing the culture bag with a breather membrane by a sealing machine, and transferring the culture bag out of a culture room for culture; constant-temperature culture: the temperature of a ten thousand-level purification culture room is set to be constant at 24 ℃, the culture is carried out for 15 days, the strains are mature, and the strains are transmitted to a constant-temperature 2 ℃ refrigeration house for storage.
Example three: agaricus bisporus strain breeding method suitable for standardized factory
Selecting high-quality and vigorous agaricus bisporus strain as mother strain, breeding, and performing fruiting experiment, and has high fruiting rate, good quality and yield of 30Kg/m2The above strains are used as parent strains of agaricus bisporus strains; adopting millet as a hypha carrier to cultivate the parent strain of the agaricus bisporus strain to prepare a solid parent strain of the agaricus bisporus strain or prepare nutrient solution to cultivate the parent strain in a liquid strain fermentation tank to prepare a liquid parent strain of the agaricus bisporus strain; screening the triticale, removing broken grains, shrunken grains and impurities, and selecting the triticale which is clean, full, complete, fresh and mildew-free; putting water into a soaking pool, dissolving quicklime to enable the pH value to reach 13.0, uniformly mixing, putting a soaking cage into the soaking pool, putting triticale into the soaking cage, stirring by using compressed air, and uniformly absorbing water of the triticale 6 times a day; testing that the water content of the triticale reaches a standard, lifting out the soaking cage by using a crane, controlling water until no obvious water drops exist, pouring the soaking cage into a stirrer, adding 1% of calcium carbonate and 0.8% of calcium sulfate by mass, uniformly stirring, adjusting the pH value to 9.0, and completing stirring; introducing the stirred triticale into a conveyor belt, entering an automatic quantitative packaging machine, quantitatively packaging half bags by using a sterilization bag, sealing by using a lantern ring and a breathing plug, and putting on a sterilization trolley, wherein each layer of 6 bags is 9 layers; high-temperature sterilization: after the sterilization trolley is filled, opening sterilization cabinet doors in a bacteria-containing area, pushing the sterilization cabinets into the sterilization cabinets, arranging the sterilization cabinets in sequence, sealing the sterilization cabinets, vacuumizing, introducing steam for sterilization, wherein the sterilization parameters are 130 ℃, 0.20MPa and 3h, exhausting and reducing pressure, opening the sterilization cabinet doors in a hundred-grade purification area on the side of a purification chamber when the pressure reaches zero, pulling out the sterilization trolley, and cooling the sterilization trolley in a hundred-grade purification cooling area for 14h to ensure that the material temperature reaches 25 ℃; and (3) sterile inoculation: transferring the cooled culture medium to ten-stage purification area, and inoculatingOpening a breather plug of a sterilization bag on an operation table, inoculating a solid stock strain of the agaricus bisporus or a liquid stock strain of the agaricus bisporus to the sterilization bag according to 1 percent of inoculation amount, shaking up, pouring the two sterilization bags into one culture bag, sealing the culture bag with a breathing film by using a sealing machine, and transferring the culture bag out of a culture room for culture; constant-temperature culture: the temperature of the ten thousand-level purification culture room is set to be constant at 24 ℃, the culture is carried out for 18 days, the strains are mature, and the strains are transmitted to a constant-temperature 2 ℃ refrigeration house for storage.
Example four: agaricus bisporus strain breeding method suitable for standardized factory
Selecting high-quality and vigorous agaricus bisporus strain as mother strain, breeding, and performing fruiting experiment, and has high fruiting rate, good quality and yield of 30Kg/m2The above strains are used as parent strains of agaricus bisporus strains; adopting millet as a hypha carrier to cultivate the parent strain of the agaricus bisporus strain to prepare a solid parent strain of the agaricus bisporus strain or prepare nutrient solution to cultivate the parent strain in a liquid strain fermentation tank to prepare a liquid parent strain of the agaricus bisporus strain; screening the triticale, removing broken grains, shrunken grains and impurities, and selecting the triticale which is clean, full, complete, fresh and mildew-free; putting water into a soaking pool, dissolving quicklime to enable the pH value to reach 12.0, uniformly mixing, putting a soaking cage into the soaking pool, putting triticale into the soaking cage, stirring by using compressed air, and uniformly absorbing water by the triticale 5 times a day; testing that the water content of the triticale reaches a standard, lifting out the soaking cage by using a crane, controlling water until no obvious water drops exist, pouring the soaking cage into a stirrer, adding 1% of calcium carbonate and 0.8% of calcium sulfate by mass, uniformly stirring, adjusting the pH value to 8.5, and completing stirring; introducing the stirred triticale into a conveyor belt, entering an automatic quantitative packaging machine, quantitatively packaging half bags by using a sterilization bag, sealing by using a lantern ring and a breathing plug, and putting on a sterilization trolley, wherein each layer of 6 bags is 9 layers; high-temperature sterilization: after the sterilization trolley is filled, opening sterilization cabinet doors in a bacteria-containing area, pushing the sterilization cabinets into the sterilization cabinets, arranging the sterilization cabinets in sequence, sealing the sterilization cabinets, vacuumizing, introducing steam for sterilization, wherein the sterilization parameter is 129 ℃, the sterilization parameter is 0.18MPa, and the sterilization is 2 hours, exhausting and reducing pressure, opening the sterilization cabinet doors in a hundred-grade purification area on the side of a purification chamber when the pressure reaches zero, pulling out the sterilization trolley, and cooling the sterilization trolley in a hundred-grade purification cooling area for 11 hours to ensure that the material temperature reaches 22 ℃; and (3) sterile inoculation: cooling the culture mediumTransferring to a ten-stage purification area, opening a sterilization bag breather plug by an inoculating worker on an operation table, inoculating a solid stock of an agaricus bisporus strain or a liquid stock of the agaricus bisporus strain to a sterilization bag according to 1 percent of inoculation amount, shaking up, pouring two sterilization bags into one culture bag, sealing the culture bag with a breathing film by a sealing machine, and transferring out to a culture room for culture; constant-temperature culture: the temperature of a ten thousand-level purification culture room is set to be constant at 24 ℃, the culture is carried out for 16 days, the strains are mature, and the strains are transmitted to a constant-temperature 2 ℃ refrigeration house for storage.
Example five: agaricus bisporus strain breeding method suitable for standardized factory
Selecting high-quality and vigorous agaricus bisporus strain as mother strain, breeding, and performing fruiting experiment, and has high fruiting rate, good quality and yield of 30Kg/m2The above strains are used as parent strains of agaricus bisporus strains; adopting millet as a hypha carrier to cultivate the parent strain of the agaricus bisporus strain to prepare a solid parent strain of the agaricus bisporus strain or prepare nutrient solution to cultivate the parent strain in a liquid strain fermentation tank to prepare a liquid parent strain of the agaricus bisporus strain; screening the triticale, removing broken grains, shrunken grains and impurities, and selecting the triticale which is clean, full, complete, fresh and mildew-free; putting water into a soaking pool, dissolving quicklime to enable the pH value to reach 11.5, uniformly mixing, putting a soaking cage into the soaking pool, putting triticale into the soaking cage, stirring by using compressed air, and uniformly absorbing water for 6 times a day; testing that the water content of the triticale reaches a standard, lifting out the soaking cage by using a crane, controlling water until no obvious water drops exist, pouring the soaking cage into a stirrer, adding 1% of calcium carbonate and 0.8% of calcium sulfate by mass, uniformly stirring, adjusting the pH value to 8.0, and completing stirring; introducing the stirred triticale into a conveyor belt, entering an automatic quantitative packaging machine, quantitatively packaging half bags by using a sterilization bag, sealing by using a lantern ring and a breathing plug, and putting on a sterilization trolley, wherein each layer of 6 bags is 9 layers; high-temperature sterilization: after the sterilization trolley is filled, opening sterilization cabinet doors in a bacteria-containing area, pushing the sterilization cabinets into the sterilization cabinets, arranging the sterilization cabinets in sequence, sealing the sterilization cabinets, vacuumizing, introducing steam for sterilization, wherein the sterilization parameters are 123 ℃, 0.15MPa and 2.5h, after sterilization is finished, exhausting and reducing pressure, opening the sterilization cabinet doors in a hundred-grade purification area on the side of a purification chamber when the pressure reaches zero, pulling out the sterilization trolley, and cooling the sterilization trolley in a hundred-grade purification cooling area for 12h to ensure that the material temperature reaches 24 ℃; is free ofAnd (3) inoculation of bacteria: transferring the cooled culture material to a ten-stage purification area, opening a sterilization bag breather plug by an inoculating worker on an operation table, inoculating a solid stock strain of the agaricus bisporus strain or a liquid stock strain of the agaricus bisporus strain to a sterilization bag according to 1 percent of inoculation amount, shaking up, pouring two sterilization bags into one culture bag, sealing the culture bag with a breather membrane by a sealing machine, and transferring the culture bag out of a culture room for culture; constant-temperature culture: the temperature of the ten thousand-level purification culture room is set to be constant at 24 ℃, the culture is carried out for 18 days, the strains are mature, and the strains are transmitted to a constant-temperature 2 ℃ refrigeration house for storage.
Example six: agaricus bisporus strain breeding method suitable for standardized factory
Selecting high-quality and vigorous agaricus bisporus strain as mother strain, breeding, and performing fruiting experiment, and has high fruiting rate, good quality and yield of 30Kg/m2The above strains are used as parent strains of agaricus bisporus strains; adopting millet as a hypha carrier to cultivate the parent strain of the agaricus bisporus strain to prepare a solid parent strain of the agaricus bisporus strain or prepare nutrient solution to cultivate the parent strain in a liquid strain fermentation tank to prepare a liquid parent strain of the agaricus bisporus strain; screening the triticale, removing broken grains, shrunken grains and impurities, and selecting the triticale which is clean, full, complete, fresh and mildew-free; putting water into a soaking pool, dissolving quicklime to enable the pH value to reach 12.5, uniformly mixing, putting a soaking cage into the soaking pool, putting triticale into the soaking cage, stirring with compressed air, and uniformly absorbing water of the triticale 4 times a day; testing that the water content of the triticale reaches a standard, lifting out the soaking cage by using a crane, controlling water until no obvious water drops exist, pouring the soaking cage into a stirrer, adding 1% of calcium carbonate and 0.8% of calcium sulfate by mass, uniformly stirring, adjusting the pH value to 7.5, and completing stirring; introducing the stirred triticale into a conveyor belt, entering an automatic quantitative packaging machine, quantitatively packaging half bags by using a sterilization bag, sealing by using a lantern ring and a breathing plug, and putting on a sterilization trolley, wherein each layer of 6 bags is 9 layers; high-temperature sterilization: after the sterilization cart is filled, opening sterilization cabinet doors of the bacteria-containing area, pushing the sterilization cabinets into the sterilization cabinets, arranging the sterilization cabinets in sequence, sealing the sterilization cabinets, vacuumizing, introducing steam for sterilization, wherein the sterilization parameters are 125 ℃, 0.12MPa and 3h, exhausting and reducing pressure, opening the sterilization cabinet doors of the hundred-grade purification area on the side of the purification chamber when the pressure reaches zero, pulling out the sterilization cart, and cooling the hundred-grade purification areaCooling for 13h to ensure that the material temperature reaches 20 ℃; and (3) sterile inoculation: transferring the cooled culture material to a ten-stage purification area, opening a sterilization bag breather plug by an inoculating worker on an operation table, inoculating a solid stock strain of the agaricus bisporus strain or a liquid stock strain of the agaricus bisporus strain to a sterilization bag according to 1 percent of inoculation amount, shaking up, pouring two sterilization bags into one culture bag, sealing the culture bag with a breather membrane by a sealing machine, and transferring the culture bag out of a culture room for culture; constant-temperature culture: the temperature of a ten thousand-level purification culture room is set to be constant at 24 ℃, the culture is carried out for 17 days, the strains are mature, and the strains are transmitted to a constant-temperature 2 ℃ refrigeration house for storage.
Example seven: process optimization of agaricus bisporus strain breeding method suitable for standardized factory
Under the implementation condition of the series of embodiments, an optimized agaricus bisporus strain breeding method is further provided, and the agaricus bisporus strain is bred by respectively adopting the following different process parameters:
the first scheme is as follows: the pH value of the soaking pool is 11.0, the compressed air is stirred for 4 times every day, the stirring pH value of the raw materials is 7.0, the sterilization temperature is 120 ℃, the sterilization pressure is 0.10MPa, the sterilization time is 2h, the cooling time is 10h, the material temperature is 18 ℃, and the culture time is 15 days.
Scheme II: the pH value of the soaking pool is 13.0, the compressed air is stirred for 6 times a day, the stirring pH value of the raw materials is 9.0, the sterilization temperature is 130 ℃, the sterilization pressure is 0.20MPa, the sterilization time is 3h, the cooling time is 14h, the material temperature is 25 ℃, and the culture time is 18 days.
The third scheme is as follows: the pH value of the soaking pool is 12.0, the compressed air is stirred for 5 times every day, the stirring pH value of the raw materials is 8.5, the sterilization temperature is 129 ℃, the sterilization pressure is 0.18MPa, the sterilization time is 2h, the cooling time is 11h, the material temperature is 22 ℃, and the culture time is 16 days.
And the scheme is as follows: the pH value of the soaking pool is 11.5, the compressed air is stirred for 6 times every day, the stirring pH value of the raw materials is 8.0, the sterilization temperature is 123 ℃, the sterilization pressure is 0.15MPa, the sterilization time is 2.5h, the cooling time is 12h, the material temperature is 24 ℃, and the culture time is 18 days.
And a fifth scheme: the pH value of the soaking pool is 12.5, the compressed air is stirred for 4 times every day, the stirring pH value of the raw materials is 7.5, the sterilization temperature is 125 ℃, the sterilization pressure is 0.12MPa, the sterilization time is 3h, the cooling time is 13h, the material temperature is 20 ℃, and the culture time is 17 days.
The five different schemes provided above are respectively used for breeding the agaricus bisporus according to the processing method provided in the first embodiment:
1. orthogonal optimization agaricus bisporus breeding process
A single-factor experiment is designed, and the influence of the pH value A of a soaking pool, the pH value B of raw material stirring and the material temperature on the yield of the agaricus bisporus in the agaricus bisporus breeding process is respectively researched. A three-factor three-level orthogonal experiment is carried out on the basis of a single-factor experiment, and the factors and levels of the orthogonal experiment are shown in Table 1.
Table 1: orthogonal test factor and horizon
The test results and analysis of the orthogonal optimization agaricus bisporus breeding process are shown in table 2.
Table 2: results and analysis of orthogonal assays
By comparing the extreme differences of the indexes, the primary and secondary sequence of the influencing factors is B>C>A, namely the pH value of raw materials for the agaricus bisporus breeding process is a main influence factor, the pH value of the raw materials and the pH value of a soaking pool are determined, and the optimal level combination of the factors is determined as A according to the values of k1, k2 and k3 of indexes in the table 2 and the results of a trend chart2B2C3Namely, the optimized process in the process of manufacturing the wheat flour comprises the following steps: the pH value of the soaking pool is 11.0-13.0, the stirring pH value of the raw materials is 7.0-9.0, and the material temperature is 18-25 ℃; preferably, the pH value of the soaking pool is 12.0, the stirring pH value of the raw materials is 8.0, and the material temperature is 25 ℃.
2. The response surface method optimizes the agaricus bisporus breeding process, and the test factors and levels are shown in table 3:
table 3: response surface test factors and horizon table
Table 4: response surface test design and results
| Numbering | Sterilization temperature/. degree.C | B sterilization pressure/Mpa | C Sterilization time/h | Percent of pass/%) |
| 1 | 125 | 0.2 | 2.0 | 78 |
| 2 | 130 | 0.2 | 2.5 | 82 |
| 3 | 120 | 0.15 | 3.0 | 76 |
| 4 | 125 | 0.2 | 3.0 | 78 |
| 5 | 120 | 0.2 | 2.5 | 84 |
| 6 | 125 | 0.15 | 2.5 | 96 |
| 7 | 130 | 0.15 | 3.0 | 83 |
| 8 | 120 | 0.15 | 2.0 | 76 |
| 9 | 125 | 0.15 | 2.5 | 94 |
| 10 | 120 | 0.1 | 2.5 | 75 |
| 11 | 125 | 0.1 | 2.0 | 79 |
| 12 | 125 | 0.15 | 2.5 | 97 |
| 13 | 125 | 0.15 | 2.5 | 96 |
| 14 | 125 | 0.15 | 2.5 | 95 |
| 15 | 130 | 0.1 | 2.5 | 78 |
| 16 | 130 | 0.15 | 2.0 | 82 |
| 17 | 125 | 0.1 | 3.0 | 79 |
Table 5: response surface test analysis of variance table
Note: indicates significance, indicates extreme significance.
Response surface test design and results:
the experimental design analysis was performed according to the Box-Benhnken center combinatorial design principle, and the results are shown in tables 4 and 5. Fitting the experimental data of 4 and 5 by adopting a multivariate fitting method through Design expert8.0.6 to obtain a quadratic polynomial regression model of the qualification rate R to the sterilization temperature A, the sterilization pressure B and the sterilization time C, wherein the quadratic polynomial regression model comprises the following steps:
R＝+95.8+1.75*A+1.38*B+0.13*C-1.25*A*B+0.25*A*C-7.65*
A^2-8.40*B^2-8.90*C^2
the interaction of the factors in the response surface analysis is described in detail with reference to figures 2 to 4. From the results of the orthogonal test and the response test for optimizing the agaricus bisporus breeding process, the optimizing process in the agaricus bisporus breeding process is as follows: the sterilization temperature is 120-130 ℃, the sterilization pressure is 0.10-0.20 MPa, and the sterilization time is 2-3 h; preferably, the sterilization temperature is 125 ℃, the sterilization pressure is 0.15MPa, and the sterilization time is 2.5 h.
By adopting the agaricus bisporus strain breeding method suitable for the standardized factory provided by the embodiment, through the orthogonal test and the response surface method optimization manufacturing process, the agaricus bisporus strain breeding method suitable for the standardized factory provided by the invention can breed high-quality and pure and high-yield agaricus bisporus cultivated species in a large batch through the response surface method optimization process, the cultivation period is greatly shortened compared with the conventional cultivation method by 30-45 days, the pollution probability is reduced, the general cultivation time of the cultivated species is 15-18 days, the black wheat is not required to be steamed, the energy is saved, the water content of the cultivated strain grains is consistent, the phenomenon of strain growth is avoided, the qualification rate can reach 96 percent, and as the third-level species are bred, no matter grains are used as solid strains or liquid strains, the two can be uniformly mixed or sprayed into a black wheat culture medium in a ten-level purification level environment, the growth points are multiple, the germination is fast, the inoculation time on each particle of the same batch of matrix is basically consistent, so the initial growth time points are consistent, the fungus ages are short, the vitality of the strains is strong, the distribution of hyphae is uniform and standard, and the method has wide practicability in the field of strain cultivation.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the design spirit of the present invention should fall within the protection scope determined by the present invention.
Claims (2)
1. A method for breeding agaricus bisporus strains suitable for a standardized factory specifically comprises the following technical steps:
(1) preparing a stock: selecting high-quality and vigorous agaricus bisporus strain as mother strain, breeding, and performing fruiting experiment, and has high fruiting rate, good quality and yield of 30Kg/m2The above strains are used as parent strains of agaricus bisporus strains; adopting millet as a hypha carrier to cultivate the parent strain of the agaricus bisporus strain to prepare a solid parent strain of the agaricus bisporus strain or prepare nutrient solution to cultivate the parent strain in a liquid strain fermentation tank to prepare a liquid parent strain of the agaricus bisporus strain;
(2) selecting raw materials: screening the triticale, removing broken grains, shrunken grains and impurities, and selecting the triticale which is clean, full, complete, fresh and mildew-free;
(3) soaking raw materials: putting water into the soaking pool, dissolving quicklime to make pH value reach 11.0-13.0, mixing well, putting a soaking cage in the soaking pool, adding triticale, stirring with compressed air for 4-6 times per day to make triticale absorb water uniformly;
(4) stirring raw materials: testing that the water content of the triticale reaches the standard, lifting out the soaking cage by using a crane, controlling water until no obvious water drops exist, pouring the soaking cage into a stirrer, adding 1% of calcium carbonate and 0.8% of calcium sulfate by mass, uniformly stirring, adjusting the pH value to 7.0-9.0, and completing stirring;
(5) and (4) quantitative packaging: introducing the stirred triticale into a conveyor belt, entering an automatic quantitative packaging machine, quantitatively packaging half bags by using a sterilization bag, sealing by using a lantern ring and a breathing plug, and putting on a sterilization trolley, wherein each layer of 6 bags is 9 layers;
(6) high-temperature sterilization: after the sterilization cart is filled, opening sterilization cabinet doors of a bacteria-containing area, pushing the sterilization cabinets into the sterilization cabinets, arranging the sterilization cabinets in sequence, sealing the sterilization cabinets, vacuumizing, introducing steam for sterilization, wherein the sterilization parameters are 120-130 ℃, 0.10-0.20 MPa and 2-3 h, after the sterilization is finished, exhausting and reducing pressure, when the pressure reaches zero, opening the sterilization cabinet doors of a hundred-grade purification area on the side of a purification chamber, pulling out the sterilization cart, and cooling the sterilization cart in the hundred-grade purification cooling area for 10-14 h to ensure that the material temperature reaches 18-25 ℃;
(7) and (3) sterile inoculation: transferring the cooled culture material to a ten-stage purification area, opening a sterilization bag breather plug by an inoculating worker on an operation table, inoculating a solid stock strain of the agaricus bisporus strain or a liquid stock strain of the agaricus bisporus strain to a sterilization bag according to 1 percent of inoculation amount, shaking up, pouring two sterilization bags into one culture bag, sealing the culture bag with a breather membrane by a sealing machine, and transferring the culture bag out of a culture room for culture;
(8) constant-temperature culture: the temperature of the ten thousand-level purification culture room is set to be constant at 24 ℃, the culture is carried out for 15 to 18 days, the strains are mature, and the strains are transmitted to a constant-temperature cold storage warehouse at 2 ℃ for storage.
2. The agaricus bisporus strain breeding method suitable for the standardized chemical plant as claimed in claim 1, wherein the pH value of the soaking pool in the raw material soaking step is 12.0, the stirring pH value of the raw material is 8.0, the material temperature is 21.5 ℃, the sterilization parameter in the high temperature sterilization step is 125 ℃, 0.15MPa, 2.5 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611264852.7A CN106676018B (en) | 2016-12-30 | 2016-12-30 | Agaricus bisporus strain breeding method suitable for standardized factory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611264852.7A CN106676018B (en) | 2016-12-30 | 2016-12-30 | Agaricus bisporus strain breeding method suitable for standardized factory |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106676018A CN106676018A (en) | 2017-05-17 |
| CN106676018B true CN106676018B (en) | 2020-07-21 |
Family
ID=58849648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611264852.7A Active CN106676018B (en) | 2016-12-30 | 2016-12-30 | Agaricus bisporus strain breeding method suitable for standardized factory |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106676018B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107602197A (en) * | 2017-10-30 | 2018-01-19 | 山东瑞蕈天库菌种开发有限公司 | A kind of preparation method of the high-quality White mushroom Cultivar culture medium of industrialized production |
| CN108812063B (en) * | 2018-08-22 | 2021-01-01 | 福建省农业科学院食用菌研究所（福建省蘑菇菌种研究推广站） | Method for preparing agaricus bisporus cultivated species by adopting synthetic matrix |
| CN109832091A (en) * | 2019-01-20 | 2019-06-04 | 香格里拉市腊玛农产品开发有限公司 | A kind of cultural method of the golden ear of half bag of mulberry wood chips plantation |
| CN115191289A (en) * | 2021-04-08 | 2022-10-18 | 临沂瑞泽生物科技股份有限公司 | Edible mushroom culture process and equipment |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000065542A (en) * | 1999-04-06 | 2000-11-15 | 강석춘 | A process for preparing a yang-song-i mushroom using horse-droppings as its raw materials |
| KR20030059775A (en) * | 2003-05-24 | 2003-07-10 | 광미실업(주) | Mushroom medium composition, mushroom medium using the same, and method for cultivating mushroom |
| KR20120091920A (en) * | 2011-02-10 | 2012-08-20 | 주식회사 에코엔 | Culture medium composition for mushroom(agaricus bisporus) with high amino acids content and its preparation method, and a method of cultivating mushroom using it |
| CN103340090A (en) * | 2012-01-09 | 2013-10-09 | 福建省农业科学院土壤肥料研究所 | Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer |
| CN104823712A (en) * | 2015-04-30 | 2015-08-12 | 上海市农业科学院 | Anti-aging agaricus bisporus wheat strain and preparation method thereof |
| CN104920070A (en) * | 2015-06-17 | 2015-09-23 | 江苏省农业科学院宿迁农科所 | Method for increasing yield of agaricus bisporus |
| CN105453895A (en) * | 2015-12-23 | 2016-04-06 | 嘉兴圣洲生物科技有限公司 | Cultivation technology for Agaricus blazei |
| CN105567572A (en) * | 2016-01-04 | 2016-05-11 | 山东省科创食用菌产业技术研究院 | Preparation technology of agaricus bisporus strain |
-
2016
- 2016-12-30 CN CN201611264852.7A patent/CN106676018B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000065542A (en) * | 1999-04-06 | 2000-11-15 | 강석춘 | A process for preparing a yang-song-i mushroom using horse-droppings as its raw materials |
| KR20030059775A (en) * | 2003-05-24 | 2003-07-10 | 광미실업(주) | Mushroom medium composition, mushroom medium using the same, and method for cultivating mushroom |
| KR20120091920A (en) * | 2011-02-10 | 2012-08-20 | 주식회사 에코엔 | Culture medium composition for mushroom(agaricus bisporus) with high amino acids content and its preparation method, and a method of cultivating mushroom using it |
| CN103340090A (en) * | 2012-01-09 | 2013-10-09 | 福建省农业科学院土壤肥料研究所 | Method for culturing agaricus bisporus kernel microbial strain, namely, kernel cultivated specie, by using white soil as filter layer |
| CN104823712A (en) * | 2015-04-30 | 2015-08-12 | 上海市农业科学院 | Anti-aging agaricus bisporus wheat strain and preparation method thereof |
| CN104920070A (en) * | 2015-06-17 | 2015-09-23 | 江苏省农业科学院宿迁农科所 | Method for increasing yield of agaricus bisporus |
| CN105453895A (en) * | 2015-12-23 | 2016-04-06 | 嘉兴圣洲生物科技有限公司 | Cultivation technology for Agaricus blazei |
| CN105567572A (en) * | 2016-01-04 | 2016-05-11 | 山东省科创食用菌产业技术研究院 | Preparation technology of agaricus bisporus strain |
Non-Patent Citations (1)
| Title |
|---|
| 《不同培养基和pH 值对双孢菇母种菌丝生长的影响》;刘刚;《现代农业科技》;20141231(第16期);摘要及第1.2、2.1、2.2、3节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106676018A (en) | 2017-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106676018B (en) | Agaricus bisporus strain breeding method suitable for standardized factory | |
| CN102845219B (en) | Cultivation technique of Pleurotus eryngii | |
| CN103588571B (en) | Culture medium and cultivation method for needle mushrooms | |
| CN104871824A (en) | Industrial needle mushroom cultivation method | |
| CN106119131B (en) | A method of alleviating excess copper and inhibits Growth of Pleurotus Mycelium | |
| CN101715696A (en) | Factory cultivation method of maitake | |
| CN104838883A (en) | Toadstool strain preparation process | |
| CN105875197A (en) | Agaricus bisporus cultivation method | |
| CN103733875A (en) | Cordyceps militaris factory production method and process | |
| CN105453894A (en) | Method for high-yield of bottle-cultivated golden mushroom | |
| CN101717309B (en) | Culture medium for straw rotting edible fungi solid strain and method for preparing solid strain | |
| CN103109678A (en) | Method for producing stock seeds of enoki mushrooms | |
| CN104186202B (en) | A kind of factorial praluction Lentinus Edodes is breathed freely the method for bacterium bag | |
| CN101720626B (en) | Culture medium formula of edible fungus second class fungus (protospecies) and making method | |
| CN104303840A (en) | Cultivating method for tray-loaded flammulina velutipes | |
| CN104557211A (en) | Special strain culture medium for mushroom liquefaction and corresponding culture method | |
| CN110547142A (en) | Method for bottle cultivation of delicious Chinese mushrooms | |
| CN104620852B (en) | Mushroom class liquefaction Spawn incubation method | |
| CN104130055A (en) | Cheap hypsizigus marmoreus culture medium | |
| CN105948864A (en) | Culture medium for indoor and outdoor cultivation of Grifola frondosa, preparation method and cultivation method | |
| CN104541983A (en) | Special seafood mushroom liquefaction spawn culture medium and corresponding culture method thereof | |
| CN109548558A (en) | A kind of implantation methods of Pleurotus citrinopileatus | |
| CN114793766A (en) | Industrialized production system for oyster mushrooms | |
| CN104557315A (en) | Strain culture medium for pleurotus geesteranus liquefaction and corresponding culture medium | |
| CN111264303B (en) | Lyophyllum fumosoroseum cultivar and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |