KR20120091920A - Culture medium composition for mushroom(agaricus bisporus) with high amino acids content and its preparation method, and a method of cultivating mushroom using it - Google Patents

Abstract

PURPOSE: A medium composition for culturing agaricus bisporus having the high content of amino acid, and a cultivation method of the agaricus bisporus using thereof are provided to obtain the high quality agaricus bisporus with improved productivity. CONSTITUTION: A medium composition for culturing agaricus bisporus having the high content of amino acid is obtained mixing 100 parts of main materials by weight as a carbon source, 3-60 parts of cattail by weight, and 0.3-5 parts of animal amino acid liquid fertilizer by weight. The main materials include 60-80wt% of rice straws, and 20-40wt% of mixed straws and wheat, barley, or their mixture.

Description

Culture medium composition for mushroom (Agaricus bisporus) with high amino acids content and its preparation method, and a method of cultivating mushroom using it

The present invention relates to a mushroom medium and a cultivation method, and more particularly, to a culture medium composition for cultivating mushrooms, a method for producing the same and a cultivation method of mushrooms using the same.

Mushroom is an edible mushroom belonging to the fungi of the fungi, and is widely consumed worldwide because of its excellent taste and aroma. Glyporin, Corioline, and Illusin contained in Mushrooms are antibacterial, Lentinacin, 5'-AMP, 5'-GMP are antithrombotic, Gamma-GHB is PHA weakening inhibitory, betaglucan In addition, RNA complexes are known to have antitumor action, which is very beneficial to health, improves the standard of living, and nowadays it is attracting attention as a well-being food.

Looking at the life history of mushrooms, it is a kind of pure parasitic fungi, which absorb nutrients from organic matter produced by decomposition of plant stumps and living organisms, forming mycelia and forming fruiting bodies. In addition, since the growth of mycelia depends on the quality of the nutrient medium, the mushroom medium (compost medium) directly affects the mycelial growth and the number of mushrooms. The fungus obtains various organic and inorganic nutrients such as nitrogen, phosphate, potassium, and calcium from the composting medium. The grown mushrooms are energy of 17kcal, water 92.5%, protein 3.9g, lipid 0.3g, and carbohydrate-saccharides based on 100g. 1.4 g, carbohydrate-fiber 0.6 g, ash 1.3 g, calcium 6.0 mg, phosphorus 88 mg, iron 1.0 mg, sodium 3 mg, potassium 550 mg, vitamin B1 0.12 mg, vitamin B2 0.48 mg, vitamin B6 0.11 mg, niacin 4.1 Mg, vitamin C 3 mg, folic acid 23.2 µg, and the like.

Therefore, to be successful in cultivating mushrooms, it is necessary to make the medium suitable for nutritional physiology and adjust the environment. The mushroom manure compost medium should contain more than 2% of organic nitrogen such as protein and amino acid, high lignin content, and various inorganic nutrients such as phosphorus, potassium and calcium. To produce 1 kg of mushrooms, 200 g of organic matter and 3.5 to 4.5 g of organic nitrogen are required. The culture medium for mushroom cultivation must contain enough nutrients that can be easily absorbed and used by fermenting microorganisms such as amino acids, glucose and fructose in order to facilitate fermentation. Even if the basic requirements of fermentation such as moisture and oxygen are satisfied, if the nutrients that the microorganisms such as cellulose, lignin, and protein are not immediately absorbed in the compost material, the heat of the compost is very late and weak.

However, in order to satisfy these conditions, the conventional mushroom badge has a low yield and low productivity due to commercialization compared to the cost put into the production of the badge, resulting in a 31.4% return on sales as of 2007 (Rural Development Administration, standard income for agricultural products each year). As a matter of fact, farming cultivated cultivated farmers is experiencing management difficulties. In addition, due to the decrease in cycle yields due to the lack of nutrients in the medium, the yield is greatly reduced in two, three, and four cycles compared to the one-cycle yield, which causes farmers to reduce the cost and yield of medium replacement. I am going through a double trouble.

Looking at the prior patent on the cultivation of mushroom mushrooms, in the case of registered patent 10-0423263, chaff is mixed in order to secure breathability, chaff itself is inexpensive and secures breathability, but it takes quite a long time for fermentation and C The / N ratio also has disadvantages in terms of microbial degradation, which is undesirable in view of the overall characteristics of fermentation medium and fermentation conditions. In addition, in this patent document, gypsum, which usually adds 1% of rice straw (3 to 5% in case of poor composting), is mixed twice as much as rice straw. It seems that the manufacturing process is also very complicated and costly and time consuming has a problem that is difficult to apply in a general farm.

The present invention has been made to solve the problems of the prior art, to provide a culture medium for mushroom mushroom cultivation and a method of producing the same, which can lower the medium composition cost while producing high-quality mushrooms with high amino acid content with high productivity. The purpose. In particular, in the present invention, paying attention to the direct relationship between the properties of the mushrooms produced and the properties of the medium to increase the nutritional properties of the medium at a low composition cost to increase the mushroom production per unit area, and increase the amino acid content of the medium It is an object of the present invention to provide a medium composition capable of producing a mushroom having a high amino acid content and a method of preparing the same.

In addition, an object of the present invention is to provide a method for cultivating a functional mushroom mushroom can produce a mushroom with high productivity and high amino acid content using the medium as described above.

One aspect of the present invention,

In the culture medium for mushroom mushroom cultivation comprising a main material, an additive material and an auxiliary material which is a carbon source,

With respect to 100 parts by weight of the main source of carbon,

3 to 60 parts by weight,

0.3-5 weight part of animal amino acid liquid ratio

It provides a culture medium composition for mushroom mushroom cultivation having a high amino acid content. In the present specification, the "main material" is mainly having a carbon source among the raw materials constituting the mushroom mushroom medium, and is generally made of rice straw, barley straw, straw, wild grass, etc., and means a basic material occupying 60% or more of the medium. In the present specification, "additional material" refers to organic and inorganic tertiary sources added to mushroom mushroom medium to supply nutrients insufficient for the main material, and "auxiliary material" refers to improving physical properties and acidity of the medium in addition to the main material and subsidiary materials. Means the material added for. The parts are preferably harvested and dried when the fibrous leaves are well formed, and then cut and mixed with the main material so that the square fibers of the parts are uniformly distributed. The animal amino acid liquid ratio is preferably used by impregnating a shell powder having a particle size of 3 to 6 mm. The main material is preferably rice straw 60 to 80% by weight; Straw, or barley straw, or mixed straw of wheat and barley straw 20 to 40% by weight, wherein the parts are preferably contained in 5 to 25 parts by weight based on 100 parts by weight of the main material.

The medium composition for mushroom mushroom cultivation having a high amino acid content of the present invention is particularly preferably,

(a) 60-80 wt% rice straw; 100 parts by weight of the main material consisting of 20 to 40% by weight of straw or barley straw or mixed straw of barley and barley straw,

(b) 8 to 15 parts by weight of the total amount; 0.3 to 0.8 parts by weight of urea; 0.3 to 0.8 parts by weight of yuan; An auxiliary material comprising 1 to 2.2 parts by weight of superphosphate lime,

(c) 3 to 60 parts by weight of parts,

(d) 0.3 to 5 parts by weight of animal amino acid liquid ratio impregnated into shell powder having a particle diameter of 3 to 6 mm

.

Another aspect of the present invention,

(a) making the culture composition for the mushroom mushroom cultivation as described above and performing the inverting operation to be deposited outdoors to maintain the water content of 70 to 75%, pH 7.5 to 8 and to reduce the initial weight by 20 to 30%; ;

(b) standing the composted compost on the cultivator, sealing the cultivator and sterilizing by heating the room temperature to 55-60 ° C. for 7-8 hours;

(c) After the sterilization is completed, the temperature of the compost is fermented for 1 to 2 days at 55 to 58 ° C. and then the self-heating of the compost is reduced, while lowering the temperature of the compost for 2 to 3 days at 45 to 50 ° C., 45 to 50 After fermentation for 1 ~ 2 days at ℃, fermentation for 7 ~ 9 days, after fermentation, when fermentation temperature reaches 45 ㅁ 2 ℃

It includes, provides a method for producing a high mushroom content mushroom cultivation compost medium.

Another aspect of the invention,

(a) lowering the temperature of the medium to 24 ± 3 ° C. and inoculating the seed after completion of the post-fermentation in the prepared compost medium;

(b) culturing the seeded seedlings at room temperature 22 ° C. ± 3 for 12 to 15 days to make the seedlings stick to the seedlings, and then performing a cover work;

(c) After 4-7 days after the cover, the hyphae floats on the cover, dilute the amino acid solution by 500 ~ 1500 times, irrigation to the level of 4 ~ 6L / 3.3㎡, and then apply the futo two or three times. Diluting the amino acid liquid ratio by 500 to 1500 times to calculate the water content of the medium to be 70 to 75% and dividing the water into 1 to 2 times per week; And

(d) after the watering is finished, lowering the room temperature to 15-18 ° C. and providing sufficient ventilation.

It includes, provides a cultivation method of mushroom mushrooms having a high amino acid content. Preferably, while growing mushrooms, the animal amino acid liquid ratio is diluted 100-1000 times and sprayed finely once or three times per week on the medium.

The present invention increases the nutritional properties of the medium at low composition cost by using the fertilizer and animal amino acid liquid fertilizer to increase the production of mushroom mushrooms per unit area, to produce a high-quality mushrooms with high amino acid content by increasing the amino acid content of the medium. The medium of the present invention is smoothly fermented, without damage caused by various germs, and as can be seen from the embodiment, it is possible to harvest a functional mushroom mushroom having a high amino acid content, and the quantity per cycle is also excellent by more than 20% compared to conventional cultivation. In addition, you can harvest mushrooms with high marketability. According to the present invention, it is possible to produce high-quality mushrooms with high amino acid content with high productivity by formulating the medium in an economical and effective way, which can greatly contribute to farm income, and produce high-quality mushrooms with excellent taste, flavor and functionality It can satisfy the needs of consumers who prefer well-being foods.

1 is a photograph taken by enlarging the fibrous tissue of the bud leaf.
Figure 2 is a photograph taken a state of cultivation of mushrooms according to the present invention.
Figure 3 is a photograph taken before harvest of the mushroom mushroom cultivated in accordance with the present invention.

There are more than 25 kinds of amino acids in mushrooms, and their contents are in the order of glutamic acid, aspartic acid, alanine, serine, threonine, leucine, and so on. It is similar in appearance to the cultured mushrooms produced. In addition, the free amino acid content of mushrooms was 37.85 mg / g in the gat area, 27.50 mg / g in the sack area, and 120.15 mg / g in the gat area and 103.13 mg / g in the bag area, compared to other nutrients. It contains an overwhelming amount of amino acids. Therefore, the composition of amino acids in the composition of the compost medium has a very important influence on the growth, shoot and quality of the mushrooms produced.

In the present invention, parts rich in amino acids, free sugars, minerals, and the like are mixed in the main material at a constant ratio and used as a medium raw material. Typha orientalis is a plant 1 ~ 1.5m high that grows at the edge of a pond or wetland. Leaves are 5 ~ 10mm wide in the shape of a butterfly and completely enclose the bottom of the stem. Distributed. In addition, the leaf of the butterfly is 1.5 ~ 2 ㎝ big toe ( T. latifolia ) is called the northern hemisphere spread in temperate regions. T. angustata differs in flower stems between male and female spikelets, and is distributed in temperate, tropic, and Mediterranean coasts of Europe, Asia. In addition, it grows up to 80-150cm in height and has smaller worms ( T. laxmanit ) than the wolves or baby chicks . In the present invention, parts, large parts, baby parts, moth parts are all available, and in the present specification, "parts" means all of them unless specifically defined. The inventor has previously filed a "water treatment method using parts and a method of utilizing cultivated parts" (published patent 10-2007-0016625). Although not particularly limited, as the parts of the present invention, the parts cultivated for water treatment can be finally used as mushroom medium using the parts according to Published Patent Application 10-2007-0016625.

They are easily available, inexpensive materials, and contain the crude protein, crude fat, crude fiber, carbohydrates, amino acids, sugars, and minerals needed for culture of mushrooms. Since mushrooms are supplied with inorganic salts such as potassium, calcium, magnesium and iron necessary for growth from the composting medium, the nutritional properties of the medium have a very important effect on the growth of mushrooms. In addition, in order for fermentation of the compost medium to occur smoothly, the amino acids contained in the compost material, nutrients such as glucose and fructose, such as fermentation microorganisms, can be easily absorbed and used. As a result of analyzing the amino acid content of the baby parts, it can be seen that the amino acids are very rich as shown in Table 1 below. Table 2 below shows the inorganic content of babies, and Table 3 shows the free sugar content of babies (Source of Tables 1 ~ 3: Koo Ja Hyung et al. 12 (2007), “Development of technology for the cultivation and utilization of wealth using wet answers, Chungnam University Agricultural and Life Sciences, Research Report on Agricultural and Forest Technology Development Project ").

In this way, the parts are easily used by microorganisms such as amino acids, sugars, minerals, and the like and contain nutrients constituting the components of mushroom mushrooms. By making it active, it is possible to reduce the amount of nitrogen released into the air and to reduce unnecessary decomposition of carbohydrates. Particularly, since the parts are rich in amino acid content, they can induce complete fermentation during fermentation of the medium, and can produce mushrooms having high amino acid content by maintaining high-quality medium rich in amino acids. In addition, by using the appropriate amount mixed with the main material of the carbon source in the appropriate amount, it is also effective to reduce the amount of the main material (such as straw) of the carbon source and to replace a part of the main material with parts. In particular, rice straw, which is used as the main ingredient, has a problem that the price is expensive due to instability of supply and demand, and the use of wealth reduces the consumption of rice straw and improves the nutritional properties of the medium, and also easily absorbed and used by microorganisms. Because it contains a lot of nutrients that can be used to increase the amount of straw or barley straw that is relatively difficult to use microorganisms than rice straw.

In the present invention, the parts used as the raw material of the medium, it is good to harvest and use when the fiber structure of the willow leaf. 1 is a magnified image of the fibrous texture of the bud leaf. Harvested parts are dried and then cut and used. Preferably, the parts are quickly dried to prevent decay, and then cut into square fibers of the parts to be uniformly distributed, and then mixed with a main material such as rice straw and used together.

The parts are included in the medium at a ratio of 3 to 60 parts by weight based on 100 parts by weight of the main material as the carbon source. Although it is possible to use the parts less than 3 parts by weight, in this case it is difficult to see the effect of the addition of parts properly, when using more than 60 parts by weight there is a problem that the content of the main material that is a relatively carbon source is less.

[Table 1]

[Table 2]

[Table 3]

In the present invention, "animal amino acid liquid fertilizer" or "amino acid liquid fertilizer" is a liquid fertilizer made from an amino acid substance obtained by enzymatic hydrolysis from a high protein animal substance such as slaughter by-products, and the fourth type of compound fertilizer under the fertilizer management method. Cost). In the present invention, the amino acid liquid ratio can be used in all of the various amino acid liquid ratios commercially available. As the amino acid liquid ratio, in particular, the organic fertilizer produced by the present application Utility Model No. 0406374 can be used. In the embodiment of the present invention, the amino acid liquid ratio produced by the apparatus described in Utility Model No. 0406374 was used, the amino acid component of the used amino acid liquid ratio is shown in Table 4 below. Animal amino acid fertilizer contains a high concentration of amino acids, it is possible to supply abundant amino acids to the medium even with a small amount.

Amino acid liquid ratio is contained in a medium by the ratio of 0.3-5 weight part with respect to 100 weight part of main materials which are a carbon source. Preferably, the amino acid liquid ratio is impregnated into a certain solid particle to be included in the medium. Especially as solid particles, shells, such as oysters and shellfish, can be grind | pulverized and used. Most preferably, it is used by impregnating amino acid liquid ratio in the shell powder ground to a particle size of 3 ~ 6mm. In this way, if the shell is impregnated with an amino acid solution, it is possible for the mushroom to use the amino acid constantly for a long time, and in the process, calcium and amino acids are combined to increase the usability of the mushroom.

The mushroom mushroom medium of the present invention can be composed of a good medium rich in amino acids at a low cost of composition by constituting the medium with the animal amino acid liquid ratio together with the above parts.

[Table 4]

In the present invention, as the main material, rice straw alone or rice straw is mixed with barley straw or straw. Rice straw is preferred as a compost material because the main components such as carbohydrate and ash are different from straw or barley straw, and have high water absorption ability, and microorganisms are easily broken down and used. On the other hand, straw or barley straw is harder than rice straw, and the absorption rate of water is low and the amount of absorption is not so large that microorganisms cannot be easily decomposed. In the present invention, it is possible to facilitate composting by rapid fermentation even if the amount of rice straw in the main material is reduced and replaced with straw or barley straw by using the parts that are easily decomposed by microorganisms in the main material. In the present invention, the main material may be composed of 100 to 50% by weight of rice straw and 0 to 50% by weight of other straw (straw, or barley straw, or mixed straw of wheat and barley straw). Considering the cost, it is most preferably made of 60 to 80% by weight of rice straw and 20 to 40% by weight of other straw, wherein the parts are preferably included in 5 to 25 parts by weight based on 100 parts by weight of the main material. As other straw, straw can be used especially.

The mushroom mushroom cultivation medium of the present invention further includes additive materials (organic tertiary source and / or inorganic tertiary source) for supplying nutrients that microorganisms can easily use in addition to the main ingredients, parts, and amino acid ratios as described above. Organic tertiary sources may include nutrients readily available to microorganisms such as rice bran and poultry. The addition of organic tertiary sources not only promotes compost fermentation, but also promotes microbial growth, thereby reducing the amount of nitrogen released into the air and reducing unnecessary decomposition of carbohydrates. Inorganic tertiary sources may include urea, yuan, superphosphate lime, slaked lime, and the like. In the case of manure and urea, it is a nitrogen source of the medium.Urea is very fast in composting, and ammonia, a product of degradation, softens parts to accelerate water absorption, promotes fermentation of compost and is used as nitrogen source of microorganisms. Will increase nutrient accumulation.

In addition, the medium of the present invention may preferably further include auxiliary materials such as gypsum, silicic acid, etc. for proper properties of the medium. Cattle compost should be non-sticky, elastic and have a good moisture content, which requires the addition of gypsum. Gypsum prevents the surface of the compost from colliding, allowing air and moisture to penetrate the inside of the wells, eliminating the stickiness of the compost, and preventing the deterioration caused by excess moisture, as well as providing essential calcium for the growth of the fungus. Calcium also has a detoxifying effect on the harmful substances produced during fermentation and compost during mushroom mycelial growth. The water-soluble silicic acid can be used to control the acidity of the medium, and when the amino acid liquid ratio is added to the medium by impregnating the powdered shell, the water-soluble silicic acid is mixed with water and evenly sprayed so that the pH of the medium is 7.2 to 7.6. good. In this case, the water-soluble silicic acid may be used at about 0.005 to 0.05 parts by weight based on 100 parts by weight of the main material.

Hereinafter, the method for producing a medium according to the present invention and a cultivation method of mushroom mushroom using the medium will be described in detail through a preferred embodiment. However, the following examples are only for illustrating the present invention in more detail, the scope of the present invention is not limited by these examples.

Preparation of the badge

(1) In the production of the medium, first, water storage was performed so that the moisture content of straw was 70 to 75%. Add straw 10 to straw 60 and straw 30 to make up the straw, and make 100 parts by weight of the main material including the parts, 350 parts by weight of water, 10 parts by weight of dried ingredients, 0.5 parts by weight of urea, 0.5 parts by weight of yuan, The medium was prepared by mixing 1.5 parts by weight of perinic acid lime. The fermentation and flipping were carried out three times with respect to the prepared medium, and 0.5 to 1 parts by weight of slaked lime was mixed and flipped twice, and the amino acid liquid ratio was impregnated into shell powder having a particle diameter of 3 to 6 mm and diluted 700 times to the final inverting compost. 0.8 parts by weight of the diluted solution is mixed. When the amino acid liquid ratio is included as a constituent of the medium, it is better to impregnate the solid particles in this way, and to use the mixture at the final stage of composting. As for the amino acid liquid ratio, 5% amino acid liquid ratio shown in Table 4 was used. After mixing the shell powder impregnated with the amino acid liquid ratio, 0.01 parts by weight of water-soluble silicic acid was mixed with water and evenly spreaded over the medium, and the pH of the medium was 7.2-7.6. It was made. When urea is mixed with compost, the rate of decomposition is very high, so adding a large amount of urea at once increases the ammonia concentration of the compost rapidly, reducing the activity of fermentation microorganisms due to gas disturbance, and releasing it into the air, resulting in large loss of nitrogen. Therefore, it is advisable to spread by one third at the time of this deposition and one and two turnovers. Dividing urea increases the total nitrogen content of the compost and increases the yield of mushrooms. After this, the main sedimentation will be carried out for about 10 to 13 days. During autumn cultivation, water supply plays an important role in fermentation. In spring cultivation, the vinyl is coated to keep warm after the deposition.

Inverting is a process for uniformly mixing the compost materials, smoothing the oxygen supply, and controlling the state of fermentation heat and moisture distribution. The fermentation conditions such as temperature, moisture and aeration are controlled by flipping during outdoor deposition. The overturning operation during outdoor sedimentation depends on the sedimentation status, but it is carried out once every 1 ~ 2, 5-8 times in total, and it is adjusted to maintain the temperature around 55 ℃.

The duration of field deposition depends on weather conditions and the composition of the medium. Generally, spring cultivation is suitable for 20 to 25 days and autumn cultivation is about 15 days (10 to 20 days). After the outdoor sedimentation, the compost is placed in the grower, where the compost is reduced by 20-30% of its initial weight, and the water content is 70-75% and the pH is 7.5-8.

(2) After finishing the granulation, seal the grower for sterilization and warm the room temperature to 60 ℃ for 7 ~ 8 hours. At this time, after the room temperature reaches 60 ℃ so that the temperature of the compost does not exceed 60 ℃ is adjusted by ventilation after 2-3 hours.

(3) After sterilization, the temperature of the compost is fermented for 1 ~ 2 days at around 55 ~ 58 ℃, then lower the temperature of the compost for 2 ~ 3 days at 50 ~ 55 ℃ as the self-heating of the compost is reduced.45 It is fermented for 1 ~ 2 days at ~ 50 ℃ and the post-fermentation process is usually carried out for 7-9 days. When the compost is around 45 ℃, check the compost to terminate the fermentation. As the compost temperature gradually decreases from 60 ° C to 45 ° C during the post-fermentation process, the microbial phase in the compost is converted from high temperature bacteria to high temperature filamentous fungi and nutrient accumulation and ammonia concentration decrease. If the temperature at the end of post-fermentation is higher than 55 ℃, the microorganism will change to high temperature bacteria, and nutrients will decompose and increase ammonia. Therefore, temperature control should be thoroughly managed during post-fermentation, so that anaerobic fermentation does not occur during this period. Ventilate the grower air.

Mushroom mushroom has a very high water content of 90-92%. When harvesting 1kg, about 0.9L of compost moisture is consumed, and when the water falls below the minimum range, the amount of 1.6 kg / 3.3㎡ decreases per 1% of moisture, so that the water content of compost at the end of post-fermentation does not exceed 70 ~ 75%. Manufacture. The physicochemical properties of the medium after completion of the post-fermentation are shown in Table 5 below.

[Table 5]

Mushroom Mushroom Cultivation

(1) After completion of the fermentation, the temperature of the medium was lowered to around 24 ° C. for the spawn inoculation, and 0.4-0.5 kg per m 2 of the spawn of 705 varieties was inoculated using the spawn inoculator using the mixed inoculation method.

(2) After completion of spawn inoculation, cover with breathable vinyl or newspaper to prevent drying, sprinkle with water, and incubate for 13 ~ 14 days so that inoculated spawn can stick to room temperature around 22 ℃. In the case of humidity, if the moisture is too wet to drop, there is a risk of breeding germs.

(3) After 13 to 14 days of incubation, the seedlings adhere to the bacteria and incubate. At this time, cover work is carried out to a thickness of 3 to 4 cm, and 5 to 6 days later, the mycelia will float on the cover soil. For one growth, the amino acid liquor in Table 4 was diluted 1000-fold and irrigated to 5L / 3.3㎡ level, followed by two or three futoes. After the earthwork, watering was carried out. At this time, the amino acid liquid ratios of Table 4 were diluted 1000 times to calculate the water content of the medium to 70 to 75%, and the water was divided for 2 days. Figure 2 is a photograph of the mushroom cultivation state according to the present embodiment.

(4) After completion of watering, the temperature and ventilation should be carried out. The room temperature should be 16 ~ 17 ℃ and allow sufficient ventilation so that CO ₂ in the room can be sufficiently replaced. After 13 days, the mushrooms were harvested. Figure 3 is a picture taken before the harvest of cultivated mushrooms.

While growing mushrooms, 5% amino acid solution was diluted 700 times in water for water control and finely sprayed on the medium for 2 to 3 times / week. As described above, the amino acid fertilizer may be mixed with the medium by impregnating the solid particles when the medium is composted and composted.However, when inoculating and cultivating the mushroom strain on the medium, it is diluted 100-1000 times with water. It is good.

The mushrooms cultivated in the present example had a yield of 59.5 kg / 3.3㎡, which was superior to 40-50 kg / 3.3㎡ of the conventional cultivation method. Was high. According to the characteristics of the medium, high-quality mushrooms with high amino acid content and excellent flavor could be harvested. Table 6 shows the results of analyzing the amino acid content of the harvested mushrooms, compared with the commercial mushrooms, Table 7 shows the flavor components of the harvested amino acids. Table 8 is a measure of the hardness of the mushrooms cultivated according to the present invention according to the storage period is shown that there is no significant change in hardness even if the storage days are long, it was confirmed that the quality during distribution.

TABLE 6

   ※One. Data analyzing the mushrooms cultivated according to the example

2. Amino Acid Average Data of Mushrooms in the Market <Korean Society of Food Science and Technology 21st Issue 1 (1989)>

[Table 7]

[Table 8]

Claims (8)

In the culture medium for mushroom mushroom cultivation comprising a main material, an additive material and an auxiliary material which is a carbon source,
With respect to 100 parts by weight of the main source of carbon,
3 to 60 parts by weight,
0.3-5 weight part of animal amino acid liquid ratio
Comprising a high amino acid content of mushroom culture medium culture. The amino acid content of claim 1, wherein the parts are harvested and dried when the fiber structure of the bud leaf is well formed and then cut and mixed with the main material so that the square fiber structure of the parts is uniformly distributed. A medium composition for cultivating high mushrooms. According to claim 1, wherein the animal amino acid liquid ratio is used by impregnating the shell powder having a particle size of 3 ~ 6mm, high amino acid content culture mushroom culture medium composition. According to claim 1, wherein the main material is rice straw 60 to 80% by weight; Straw, or barley straw, or mixed straw of wheat and barley straw 20 to 40% by weight, containing about 5 to 25 parts by weight with respect to 100 parts by weight of the main material, high amino acid mushroom culture medium Composition. (a) 60-80 wt% rice straw; 100 parts by weight of the main material consisting of 20 to 40% by weight of straw or barley straw or mixed straw of barley and barley straw,
(b) 8 to 15 parts by weight of the total amount; 0.3 to 0.8 parts by weight of urea; 0.3 to 0.8 parts by weight of yuan; An auxiliary material comprising 1 to 2.2 parts by weight of superphosphate lime,
(c) 3 to 60 parts by weight of parts,
(d) 0.3 to 5 parts by weight of animal amino acid liquid ratio impregnated into shell powder having a particle diameter of 3 to 6 mm
Comprising a high amino acid content of mushroom culture medium culture. (a) After making the culture medium composition for mushroom mushroom cultivation according to any one of claims 1 to 5, while performing the flipping operation, it is deposited outdoors to maintain a water content of 70 to 75%, pH 7.5 to 8 and initial weight of 20 to 30 An outdoor deposition step of reducing%;
(b) standing the composted compost on the cultivator, sealing the cultivator and sterilizing by heating the room temperature to 55-60 ° C. for 7-8 hours;
(c) After the sterilization is completed, the temperature of the compost is fermented for 1 to 2 days at 55 to 58 ° C. and then the self-heating of the compost is reduced, while lowering the temperature of the compost for 2 to 3 days at 45 to 50 ° C., 45 to 50 After fermentation for 1 ~ 2 days at ℃, fermentation for 7 ~ 9 days, after fermentation, when fermentation temperature reaches 45 ㅁ 2 ℃
Comprising a high amino acid content of mushroom mushroom cultivation method for producing a compost. (a) lowering the temperature of the medium to 24 ± 3 ° C. and inoculating the seed after the fermentation of the compost medium prepared by the method of claim 6 is completed;
(b) culturing the seeded seedlings at room temperature 22 ° C. 3 for 12 to 15 days, tackling the seedlings on the seedlings, and then performing cover work;
(c) After 4-7 days after the cover, the hyphae floats on the cover, dilute the amino acid solution by 500 ~ 1500 times, irrigation to the level of 4 ~ 6L / 3.3㎡, and then apply the futo two or three times. Diluting the amino acid liquid ratio by 500 to 1500 times to calculate the water content of the medium to be 70 to 75% and dividing the water into 1 to 2 times per week; And
(d) after the watering is finished, lowering the room temperature to 15-18 ° C. and providing sufficient ventilation.
Containing, cultivation method of high mushroom content mushrooms. 8. The cultivation method according to claim 7, further comprising diluting the animal amino acid liquid ratio 100 to 1000 times while growing the mushrooms and spraying finely once or three times per week on the medium.

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