CN110547142A - Method for bottle cultivation of delicious Chinese mushrooms - Google Patents

Method for bottle cultivation of delicious Chinese mushrooms Download PDF

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Publication number
CN110547142A
CN110547142A CN201910949508.9A CN201910949508A CN110547142A CN 110547142 A CN110547142 A CN 110547142A CN 201910949508 A CN201910949508 A CN 201910949508A CN 110547142 A CN110547142 A CN 110547142A
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China
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bottle
mushroom
mushrooms
cultivation
delicious
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CN201910949508.9A
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徐珍
尚晓冬
张美彦
章炉军
王瑞娟
宋春艳
谭琦
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Shanghai Academy of Agricultural Sciences
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Shanghai Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/40Cultivation of spawn
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor
    • A01G18/64Cultivation containers; Lids therefor
    • A01G18/68Cultivation bottles

Abstract

The invention relates to a method for bottle cultivation of delicious Chinese mushrooms, which comprises the following steps: (1) preparing a mother seed: transferring the delicious mushroom culture strain in China to a fresh PDA test tube inclined plane, culturing in a dark place at 22-24 ℃, and obtaining a mother strain when hyphae grow over the test tube; (2) preparing a cultivated species: inoculating the mother seeds prepared in the step (1) into a cultivar bottle, and culturing for 28-32 days at 24 ℃ in a dark place to obtain cultivars; (3) preparing a bacterium bottle: inoculating the cultivated species obtained in the step (2) into a bacteria bottle, and culturing at 22-24 ℃ in the dark, wherein hyphae grow for 38-42 d; (4) and (3) cultivating and managing fruiting: and (4) when the mycelium culture is finished in the fungus bottle in the step (3), performing mycelium stimulation treatment, inducing fruiting and development management on fruiting in sequence. The method for bottle cultivation of the delicious Chinese mushrooms, disclosed by the invention, takes the pleurotus eryngii mushroom residues as a main culture medium, is short in cultivation period and high in cyclic utilization rate, and has the advantages of low cost, high efficiency, environmental friendliness and the like.

Description

Method for bottle cultivation of delicious Chinese mushrooms
Technical Field
The invention belongs to the technical field of edible mushroom cultivation, and particularly relates to a method for bottle cultivation of delicious Chinese mushrooms.
Background
The delicacy mushroom (Agaricus sinodeliciosus) in china, as shown in fig. 1, is a large edible fungus which is peculiar, very delicious and rare in our country and is named after the identification by wanozuoren, which is equal to 2015. The wild delicious Chinese mushroom fruiting body has strong fragrance and delicious taste, and not only has rich nutrition, but also has high medicinal value. The delicious mushroom in China is a new edible mushroom variety with market potential. At present, the successful cultivation of delicious mushrooms in China has been reported by a bag cultivation mode using wheat straws and rice straws as main substrates. However, the cultivation of the delicious mushroom in china in the bag cultivation mode has several disadvantages as follows: firstly, primordia can appear around the fungus bag, so that fruiting sites are indefinite; secondly, after the primordia appear, the fungus bags need to be cut off manually, otherwise, the primordia cannot be differentiated to form sporocarp, and the labor cost is high; thirdly, the fungus bags can not be reused, and the waste fungus bags are not easy to degrade and cause pollution to the environment. In addition, materials such as wheat straw, straw and the like required by the bag cultivation mode are also required by the cultivation of agaricus bisporus, but the current delicious agaricus bisporus in China is not as good as the agaricus bisporus in industrial maturity and market share, and the competition of production resources is lack of competitiveness.
In the face of the situation, new cultivation modes and cultivation materials need to be developed to meet the requirements of industrialized and large-scale production of the delicious mushrooms in China. The bottle cultivation mode is widely applied to industrial cultivation of edible mushrooms such as flammulina velutipes, pleurotus eryngii and Hypsizygus marmoreus and has the advantages of large cultivation scale, high mechanization degree and high production efficiency, but the technical scheme of applying the bottle cultivation mode to the Chinese delicious mushrooms is not reported at present. In addition, the pleurotus eryngii dregs contain rich carbon sources and nitrogen sources, and the component content can meet the requirement of cultivating delicious mushrooms in China. Therefore, how to cultivate the Chinese delicious mushroom by using the bottle cultivation mode and the pleurotus eryngii dregs to develop a new cultivation mode and a new cultivation material, further promote the industrialized and large-scale production of the Chinese delicious mushroom, and promote the rapid development of the industry is a technical problem to be solved urgently by related researchers.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a method for bottle-planting delicious Chinese mushrooms.
In order to achieve the purpose, the technical scheme of the invention is as follows:
A method for bottle cultivation of delicious Chinese mushrooms comprises the following steps:
(1) preparing a mother seed: transferring the delicious mushroom culture strain in China to a fresh PDA test tube inclined plane, culturing in a dark place at 22-24 ℃, and obtaining a mother strain when hyphae grow over the test tube;
(2) Preparing a cultivated species: inoculating the mother seeds prepared in the step (1) into a cultivar bottle, and culturing for 28-32 days at 24 ℃ in a dark place to obtain cultivars, wherein the cultivar bottle is a 650mL glass bottle;
(3) Preparing a bacterium bottle: inoculating the cultivated species obtained in the step (2) into a strain bottle, wherein the strain bottle is a 1100mL high-temperature resistant plastic bottle, and culturing at 22-24 ℃ in a dark place, and the hyphae grow for 38-42 d;
(4) and (3) cultivating and managing fruiting: after mycelium culture is completed in the fungus bottle in the step (3), performing mycelium stimulation treatment, mushroom induction and growth management on fruiting in sequence;
The mycelium stimulation treatment method comprises the following steps: opening the bottle cap, scraping the surface of the bottle mouth, inoculating the seeds and flattening the surface of the seeds;
the method for inducing fruiting comprises the following steps: transferring the mushroom bottle subjected to mushroom scratching treatment into a mushroom outlet room, controlling the temperature in the mushroom outlet room to be 20-22 ℃, controlling the relative humidity of air to be more than 90%, controlling the carbon dioxide concentration to be 500-;
The development management method comprises the following steps: after most of the bacteria are grown, adjusting the temperature to be 15-18 ℃, the relative humidity of air to be 95-99 percent and the illumination intensity to be 500-;
When the bagged mode is adopted for cultivating the delicious Chinese mushrooms in the prior art, compared with the bottle cultivation mode, the air permeability and the light transmittance of the bag cultivation fungus bags are higher than those of the fungus bottles, so that primordia are easily formed on the side surfaces of the fungus bags during the induction fruiting, and the fruiting sites are indefinite; for the cultivation of the fungus bottle, because the oxygen and the illumination on the side surface are insufficient, the primordium is not easy to form, and the sufficient oxygen and the illumination are provided on the upper part of the bottle mouth, the primordium is favorably formed, and the fruiting site is determined.
The PDA test tube, the cultivated species bottle and the bacteria bottle are sterilized before use, so that the mixed bacteria pollution is prevented.
the preferable technical scheme is as follows:
in the method for bottle cultivation of delicious Chinese mushrooms, in the step (1), the formula of the culture medium in the PDA test tube is as follows: 200g of potato, 20g of glucose and 20g of agar, adding water to 1000mL, and ensuring that the pH value is natural, and the specification of a PDA test tube is 200mm multiplied by 20 mm.
In the method for bottle cultivation of delicious Chinese mushrooms, in the step (2), the formula of the culture material in the cultivation bottle is as follows: 99% of wheat grains, 1% of gypsum powder, 62% of humidity and natural pH value.
According to the method for bottle cultivation of delicious Chinese mushrooms, in the step (3), the formula of the culture material in the mushroom bottle is as follows: 59% of pleurotus eryngii fungus residues, 20% of straws, 20% of bran, 1% of gypsum powder, 62% of humidity and natural pH value;
the pleurotus eryngii mushroom dregs contain rich carbon sources and nitrogen sources, the cultivation requirements of delicious mushrooms in China can be met in terms of component content, the pleurotus eryngii mushroom dregs are used as a main culture medium, the pleurotus eryngii mushroom dregs do not occupy production resources of other edible mushroom cultivation, and the effective cyclic utilization of agricultural resources is facilitated.
according to the method for bottle cultivation of the delicious Chinese mushrooms, the pleurotus eryngii dregs are screened before use. Impurities are removed by screening, and the lumpy mushroom dregs are smashed, so that the growth of hyphae is uniformly distributed and the growth speed is consistent.
in the method for bottle cultivation of delicious Chinese mushrooms, after the first tide of mushrooms is harvested, the second tide of mushrooms is continuously cultivated and subjected to fruiting cultivation management, and the fruiting cultivation management method of the second tide of mushrooms is the same as that of the first tide of mushrooms.
according to the method for bottle cultivation of the delicious Chinese mushrooms, after the first tide of mushrooms is harvested, the temperature is adjusted to 20-23 ℃, the humidity is adjusted to 90%, the light intensity is adjusted to 100-500Lx, and then the fruiting cultivation management of the second tide of mushrooms is carried out.
according to the method for bottle cultivation of the delicious Chinese mushrooms, the cultivation period of the first tide of mushrooms is 58-68 d.
the invention also discloses a delicious Chinese mushroom prepared by the method for bottle cultivation of the delicious Chinese mushroom, which is milky white in color, round in shape and without opening the mushroom, wherein the surface of a pileus is partially covered with fine scales, and a circle of pileus is arranged at the part of a stipe close to the pileus.
the delicious mushrooms of China as described above had an average diameter of 65mm and a height of 55mm, and an average individual mushroom weight of 45 g.
Has the advantages that:
(1) compared with the traditional bag cultivation mode, the method for bottle cultivation of the delicious Chinese mushrooms can regulate and control fruiting sites, so that fruit bodies grow above the bottle openings.
(2) Compared with the traditional bag cultivation mode, the method for bottle cultivation of the delicious Chinese mushrooms adopts the fungus bottles to replace the fungus bags, the fungus bottles are firm and durable, can be recycled, reduces the pollution to the environment, and solves the problems that the fungus bags in the bag cultivation mode cannot be reused, and the waste fungus bags are not easy to degrade and easily cause the pollution to the environment;
(3) according to the method for bottle cultivation of the delicious Chinese mushrooms, the pleurotus eryngii mushroom residues are used as a main culture medium, the pleurotus eryngii mushroom residues contain rich carbon sources and nitrogen sources, the cultivation requirements of the delicious Chinese mushrooms can be met on the aspect of component content, production resources for cultivation of other edible mushrooms cannot be occupied, and effective cyclic utilization of agricultural resources is facilitated;
(4) According to the method for bottle cultivation of the delicious Chinese mushrooms, the cultivation period from inoculation to first-tide mushroom harvesting is 58-68d, compared with the traditional bagged cultivation method for the delicious Chinese mushrooms, the method avoids the link of composting secondary fermentation materials, is short in cultivation period, improves the cyclic utilization rate, has the advantages of low cost, high efficiency, environmental friendliness and the like, is expected to promote industrial and large-scale production of the delicious Chinese mushrooms, and promotes the rapid development of the industry;
(5) the delicious Chinese mushroom cultivated by the bottle cultivation method of the delicious Chinese mushroom has excellent properties, the average diameter can reach 65mm, the height is 55mm, the average single mushroom can reach 50g, and the yield and the quality are improved.
Drawings
FIG. 1 is a fruit body of a wild delicious mushroom of China;
FIG. 2 is a fruit body of a first tide of delicious Chinese mushroom cultivated by a method for bottle cultivating the delicious Chinese mushroom according to the present invention;
FIG. 3 is a fruit body of the first tide of delicious mushroom in China cultivated by a bag cultivation method.
Detailed Description
the invention will be further illustrated with reference to specific embodiments. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
the tested material is Chinese delicious mushroom, and the strain number is CCTCC NO: m2019187 (wild separation and domestication), the cultivation method is fungus bottle cultivation, and the cultivation material is a culture medium mainly comprising Pleurotus eryngii dregs.
example 1
A method for bottle cultivation of delicious Chinese mushrooms comprises the following specific steps:
(1) Preparing a mother seed: transferring the delicious mushroom culture strain in China to a fresh PDA test tube inclined plane, culturing in a dark place at 22-24 ℃, and obtaining a mother strain when hyphae grow over the test tube, wherein the formula of a culture medium in the PDA test tube is as follows: 200g of potato, 20g of glucose and 20g of agar, and adding water to 1000mL, wherein the pH value is natural;
(2) Preparing a cultivated species: inoculating the mother seeds prepared in the step (1) into a cultivated species bottle, and culturing for 28-32 days at 24 ℃ in a dark place to obtain cultivated species, wherein the formula of the culture materials in the cultivated species bottle is as follows: 99% of wheat grains, 1% of gypsum powder, 62% of humidity and natural pH value;
(3) Preparing a bacterium bottle: inoculating the cultivated species obtained in the step (2) into a fungus bottle, wherein the formula of the culture material in the fungus bottle is as follows: 59% of pleurotus eryngii mushroom residues, 20% of straws, 20% of bran and 1% of gypsum powder, wherein the pH value is natural, each bottle averagely contains 300g of culture materials, the bottles are placed in an environment with the temperature of 22-24 ℃ and the humidity of 62% and are cultured in a dark place, hyphae grow for 38-42d, and the pleurotus eryngii mushroom residues are screened before use;
(4) And (3) cultivating and managing fruiting: after mycelium culture is completed in the fungus bottle in the step (3), performing mycelium stimulation treatment, mushroom induction and growth management on fruiting in sequence;
The mycelium stimulation treatment method comprises the following steps: opening the bottle cap, scraping the surface of the bottle mouth, inoculating the seeds and flattening the surface of the seeds;
the method for inducing fruiting comprises the following steps: transferring the mushroom bottle subjected to mushroom scratching treatment into a mushroom outlet room, controlling the temperature in the mushroom outlet room to be 20-22 ℃, controlling the relative humidity of air to be more than 90%, controlling the concentration of carbon dioxide to be 500-;
The development management method comprises the following steps: adjusting the temperature to 15-18 ℃, the relative air humidity to 95-99% and the illumination intensity to 500-;
After the first tide of mushrooms are harvested, the temperature is increased to 20-23 ℃, the humidity is reduced to 90%, the light intensity is adjusted to 100-500Lx, and the second tide of mushrooms are continuously cultured for fruiting cultivation management, wherein the fruiting cultivation management method of the second tide of mushrooms is the same as that of the first tide of mushrooms;
The cultivation period from inoculation to first harvest of the mushrooms in the method for bag cultivation of the delicious mushrooms in China is 58-68 d;
as shown in figure 2, the delicious Chinese mushroom prepared by the method is milky white, round in shape, not opened, partially covered with fine scales on the pileus surface, and has a circle of pileus close to the pileus part, the average diameter is 65mm, the height is 55mm, and the average weight of a single mushroom is 45 g.
Comparative example 1
the method for cultivating the delicious Chinese mushrooms in the bag cultivation mode comprises the following specific steps:
(1) Preparing a mother seed: transferring the delicious mushroom culture strain in China to a fresh PDA test tube inclined plane, culturing in a dark place at 22-24 ℃, and obtaining a mother strain when hyphae grow over the test tube, wherein the formula of a culture medium in the PDA test tube is as follows: 200g of potato, 20g of glucose and 20g of agar, and adding water to 1000mL, wherein the pH value is natural;
(2) Preparing a cultivated species: inoculating the mother seeds prepared in the step (1) into a cultivated species bottle, and culturing for 28-32 days at 24 ℃ in a dark place to obtain cultivated species, wherein the formula of the culture materials in the cultivated species bottle is as follows: 99% of wheat grains, 1% of gypsum powder, 62% of humidity and natural pH value;
(3) composting the secondary fermentation material: taking wheat straws and chicken manure as main raw materials, and performing conventional primary fermentation and secondary fermentation according to the stacking requirement of the agaricus bisporus cultivation material, wherein 24 days are required for stacking completion;
(4) Preparing a bag cultivation fungus bag: inoculating the cultivated species obtained in the step (2) into a fungus bag, wherein the formula of the culture material in the fungus bag is as follows: 79% of secondary fermentation material, 20% of bran, 1% of gypsum powder, 62-65% of humidity, natural pH value, 500g of culture material per average, inoculating, and culturing in a dark place in an environment with the temperature of 22-24 ℃ and the humidity of 62%;
(5) And (3) cultivating and managing fruiting: after the mycelium culture is finished in the fungus bags in the step (4), sequentially carrying out induced fruiting and development management;
The method for inducing fruiting comprises the following steps: the fungus bag is moved into a mushroom growing room, the temperature in the mushroom growing room is controlled to be 20-22 ℃, the relative humidity of air is more than 90%, the concentration of carbon dioxide is 500-1000ppm, and the illumination intensity is 100-500Lx until primordium appears;
The development management method comprises the following steps: adjusting the temperature to 15-18 ℃, the relative air humidity to 95-99% and the illumination intensity to 500-;
after the first tide of mushrooms are harvested, the temperature is increased to 20-23 ℃, the humidity is reduced to 90%, the light intensity is adjusted to 100-500Lx, and the second tide of mushrooms are continuously cultured for fruiting cultivation management, wherein the fruiting cultivation management method of the second tide of mushrooms is the same as that of the first tide of mushrooms;
The method for bag cultivation of delicious Chinese mushrooms has a cultivation period from inoculation to first-tide mushroom harvest of 50-55 d;
The finally prepared delicious Chinese mushroom is milky white as shown in fig. 3, brown pigment is locally visible on the surface of a pileus pressed by a mushroom bag, the mushroom shape is round, fruiting bodies on the side surfaces of the mushroom bag are inclined, the average diameter is 70mm, the height is 60mm, and the average weight of single mushroom is 60 g.
Comparing the method for cultivating the delicious mushrooms in china in the bottle cultivation mode and the bag cultivation mode respectively and the finally prepared fruit bodies in the above example 1 and comparative example 1, as shown in table 1, it can be seen from table 1 that the cultivation period of the delicious mushrooms in china is greatly reduced because the link of composting secondary fermentation material is avoided in the bottle cultivation mode compared with the bag cultivation mode; when the bagging mode is adopted, fungus bags around the primordium need to be cut off manually, and the bottling mode avoids the link and reduces the labor cost; the Chinese delicious mushroom fruiting body obtained by adopting the bag cultivation mode has the characteristics that brown pigment is visible on the part of the pileus, the mushroom type part is inclined, the fruiting body obtained by adopting the bottle cultivation mode is milky white in pileus, has no pigment precipitation, is round in mushroom type, and is greatly improved in quality; moreover, compared with the bag cultivation mode, the Chinese delicious mushroom cultivated in the bottle cultivation mode has higher biological efficiency and better stress resistance.
TABLE 1 comparison of delicious mushrooms cultivated in China in different modes
Bag cultivation mode Bottle planting mode
time (day) required for composting secondary fermentation material 24 0
time (min) required for cutting single bag/bottle 10 0
Time (min) required for single-package/bottle mycelium stimulation 0 1
labor and time costs Is higher than Is lower than
Color of fruiting body Opalescent, locally visible brown pigment of pileus milk white
Mushroom shape Is more rounded and partially deflected round and tidy
Single bag/bottle yield (g) 120 90
biological efficiency (%) 63 79
Stress resistance In general is preferably used

Claims (10)

1. a method for bottle cultivation of delicious Chinese mushrooms is characterized by comprising the following steps:
(1) preparing a mother seed: transferring the delicious mushroom culture strain in China to a fresh PDA test tube inclined plane, culturing in a dark place at 22-24 ℃, and obtaining a mother strain when hyphae grow over the test tube;
(2) Preparing a cultivated species: inoculating the mother seeds prepared in the step (1) into a cultivar bottle, and culturing for 28-32 days at 24 ℃ in a dark place to obtain cultivars;
(3) Preparing a bacterium bottle: inoculating the cultivated species obtained in the step (2) into a bacteria bottle, and culturing at 22-24 ℃ in the dark, wherein hyphae grow for 38-42 d;
(4) And (3) cultivating and managing fruiting: after mycelium culture is completed in the fungus bottle in the step (3), performing mycelium stimulation treatment, mushroom induction and growth management on fruiting in sequence;
the mycelium stimulation treatment method comprises the following steps: opening the bottle cap, scraping the surface of the bottle mouth, inoculating the seeds and flattening the surface of the seeds;
The method for inducing fruiting comprises the following steps: transferring the mushroom bottle subjected to mushroom scratching treatment into a mushroom outlet room, controlling the temperature in the mushroom outlet room to be 20-22 ℃, controlling the relative humidity of air to be more than 90%, controlling the carbon dioxide concentration to be 500-;
The development management method comprises the following steps: adjusting the temperature to 15-18 ℃, the relative air humidity to 95-99% and the illumination intensity to 500-.
2. The method for bottle cultivation of delicious mushrooms according to claim 1, wherein in step (1), the formula of the culture medium in the PDA test tube is as follows: 200g of potato, 20g of glucose and 20g of agar, and water is added to 1000mL, so that the pH value is natural.
3. The method for bottle cultivation of delicious mushrooms of China as claimed in claim 1, wherein in step (2), the formula of the compost in the cultivation bottle is as follows: 99% of wheat grains, 1% of gypsum powder, 62% of humidity and natural pH value.
4. the method for bottle cultivation of delicious mushrooms of China according to claim 1, wherein in the step (3), the formula of the compost in the mushroom bottle is as follows: 59% of pleurotus eryngii fungus residues, 20% of straws, 20% of bran and 1% of gypsum powder.
5. The method for bottle cultivation of delicious Chinese mushrooms according to claim 4, wherein the Pleurotus eryngii dregs are screened before use.
6. The method for bottle cultivation of delicious mushrooms of China as claimed in claim 1, wherein after the first tide of mushrooms is harvested, the second tide of mushrooms is cultivated and cultivated for fruiting, and the second tide of mushrooms is cultivated and managed in the same way as the first tide of mushrooms.
7. The method as claimed in claim 6, wherein the temperature is adjusted to 20-23 ℃ and the humidity is adjusted to 90% before the second tide of mushrooms is grown and managed, and the light intensity is adjusted to 100-500 Lx.
8. The method for bottle cultivation of delicious mushrooms of China as claimed in claim 1, wherein the cultivation period from inoculation to harvest of the first tide of mushrooms is 58-68 d.
9. the delicious Chinese mushroom produced by the method for bottle-cultivation of delicious Chinese mushroom according to any one of claims 1 to 8, wherein the color is milky white, the shape of the mushroom is round, the mushroom is not opened, the surface of the pileus is partially covered with fine flakes, and the portion of the stipe near the pileus is provided with a circle of pileus.
10. the deliciously mushroom of China according to claim 8, wherein the average diameter is 65mm, the height is 55mm and the average weight of the single mushroom is 45 g.
CN201910949508.9A 2019-10-08 2019-10-08 Method for bottle cultivation of delicious Chinese mushrooms Pending CN110547142A (en)

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CN113141968A (en) * 2021-03-02 2021-07-23 上海市农业科学院 Method for cultivating delicious Chinese mushrooms by cutting-free water-retaining film bags
CN113999775A (en) * 2021-10-28 2022-02-01 浙江大学 Chinese delicious mushroom of chaihu and application thereof

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CN112166953A (en) * 2020-10-26 2021-01-05 临沂大学 Method for cultivating ornamental pleurotus eryngii through fruiting in bottle
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CN113141968A (en) * 2021-03-02 2021-07-23 上海市农业科学院 Method for cultivating delicious Chinese mushrooms by cutting-free water-retaining film bags
CN113999775A (en) * 2021-10-28 2022-02-01 浙江大学 Chinese delicious mushroom of chaihu and application thereof

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