CN106305131A - Morchella sunlight greenhouse bionic environment high-yield cultivation method - Google Patents

Morchella sunlight greenhouse bionic environment high-yield cultivation method Download PDF

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Publication number
CN106305131A
CN106305131A CN201610646048.9A CN201610646048A CN106305131A CN 106305131 A CN106305131 A CN 106305131A CN 201610646048 A CN201610646048 A CN 201610646048A CN 106305131 A CN106305131 A CN 106305131A
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morchella
pers
heliogreenhouse
soil
morchella esculenta
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Inventor
毛晓军
师立伟
杨峰
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Dingxi Yuanshun Biotechnology LLC
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Dingxi Yuanshun Biotechnology LLC
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

Abstract

The invention relates to a morchella sunlight greenhouse bionic environment high-yield cultivation method, which comprises the following steps of (1) preparing a liquid culture medium; (2) performing morchella stem removal on strongly growing morchella sporocarps with great size and good growth condition in wild environment; sanitizing rest pileuses and then performing slicing; inoculating the morchella in the liquid culture medium for culture to obtain a mother culture medium; (3) preparing a cultivation culture medium; (4) injecting the mother culture medium in each bag of cultivation culture medium for inoculation beside the flame of an alcohol lamp; (5) performing morchella fermentation after the inoculation completion, and obtaining white hyphae after 15 days; (6) selecting a large-span upright-post-free sunlight greenhouse, maintaining the soil pH value to 7 to 9, and meanwhile, enabling the soil humidity to be 30 to 40 percent; (7) performing ridging seeding; (8) performing morchella fermentation management; (9) performing harvesting: when the morchella covers grow to 4 to 7cm, lamellae on the morchella cover gradually expand, the harvesting can be performed. The method has the advantages that the operation is simple; the large-area demonstration and promotion is easy; high-yield and efficient cultivation is realized through whole-process bionic environment simulation.

Description

A kind of Morchella esculenta (L.) Pers heliogreenhouse bionical border high yield cultivating method
Technical field
The present invention relates to the cultural method of a kind of Morchella esculenta (L.) Pers, particularly relate to a kind of Morchella esculenta (L.) Pers heliogreenhouse bionical border high yield and plant Culture method.
Background technology
Morchella esculenta (L.) Pers (Morchella spp) is commonly called as Gaster caprae seu Ovis dish, Gaster caprae seu Ovis mushroom, analyzes and shows that Morchella esculenta (L.) Pers is rich in the 8 of needed by human Planting aminoacid and vitamins, particularly Morchella esculenta (L.) Pers contain C-3-Amino-L-proline, aminoisobutyric acid and 2,4-diaminourea is different The rare amino acids such as butanoic acid, thus there is its peculiar flavour, it is one of the most foremost Precious Edible Fungi.The artificial of it is tamed and dociled Change plantation always one of home and abroad fungus scholar's problem endeavouring research and probe.
The research history of Morchella esculenta (L.) Pers the most nearly 100 years, content is more.In recent years, the domestic research to Morchella esculenta (L.) Pers also had many Piece report, mainly includes that resource survey, strain separating are identified and the test of sclerotium occurrence condition, Morchella esculenta (L.) Pers field production etc., but Fruiting poor stability and yield are relatively low.One, the most tame Morchella esculenta (L.) Pers nineteen eighty-two, Ower Morchella crassipes (M.crassipes) sclerotium carries out fruiting experiment as trophosome, obtains Morchella esculenta (L.) Pers first under manual control condition Ascocarp, it is understood that sclerotium plays a crucial role in Morchella esculenta (L.) Pers life cycle.Two, the Morchella esculenta (L.) Pers life cycle figure described first: 1990 Year, in the Morchella esculenta (L.) Pers life cycle of Thomas research, the cell suspending line in each stage, depicts the life cycle figure of Morchella esculenta (L.) Pers first.This Individual achievement is the most particularly significant to studying of both Morchella esculenta (L.) Pers genetic breeding and artificial culture.
Morchella esculenta (L.) Pers is typically grown in the forest zone of height above sea level 2100 ~ 3400 meters, because of tighter to the environment required by growth promoter Lattice, therefore realizing artificial growth has certain difficulty.
At present, general cultural method is as follows:
(1) plant formulation: 1. crops straw powder 74.5%, wheat bran 20%, phosphate fertilizer 1%, Gypsum Fibrosum 1%, Calx 0.5%, fertile soil 3%;② Wood flour 75%, wheat bran 20%, phosphate fertilizer 1%, Gypsum Fibrosum 1%, fertile soil 3%;3. boll hull 75%, wheat bran 20%, Gypsum Fibrosum 1%, Calx 1%, fertile soil 3%.Three of the above, chooses any one kind of them.Material-water ratio is 1:1.3, mixes heap fermentation 20 days after honest material, and water content is 60%.Use 17 cm x 33 centimetres polypropylene or vinyon sacked materials, with sacked material 500 ~ 600 grams, then go out under the conditions of 100 DEG C Bacterium 8 hours, i.e. can be accessed by strain after sterilizing.Use two inocalation method, seal bag mouth, be placed at 22 ~ 25 DEG C cultivation about 30 days, Mycelia can long purseful.After mycelia purseful 5 ~ 6 days, make it the most considerable, can cultivate.
(2) indoor de-bag cultivating:
Can cultivate after mushroom house sterilization.First at one block of every layer of bed surface upper berth plastic sheeting, then repave the humic of 3 cm thicks Soil, arranges in bed one by one by the bacterium rod sloughing plastic bag after clapping, and general 1 square metre of bed surface can arrange 17 cm x 33 centimetres 40, plastics bacterium bag.After drained bacterium rod light water spray 1 time can earthing 3 ~ 5 centimetres, earthing rear surface cover again 2 cm thicks Folium Bambusae or Broad leaf tree is fallen leaves, and keeps ground moistening.Sporophore can be grown after 1 month.Between general southern area March 10 to April 20 Fruiting is optimal.Now air humidity is 85% ~ 90%.Within after Morchella esculenta (L.) Pers is unearthed 7 ~ 10 days, with regard to energy growth and maturity, general color is by dark-grey Complexion changed becomes light grey or isabelline just can gather.
(3) outdoor de-bag cultivating:
The forest land bedding selecting illumination to be three points of sun seven seconds.Furrow are wide 1 meter, and deep 15 ~ 20 centimetres, length does not limits.After whole good furrow spray or Gently water once, and kill insect and miscellaneous bacteria in furrow with 10% lime water.De-bag discharge of bacteria rod method and management of producing mushroom method are with indoor Cultivating identical, simply bottom can not paving plastic film, it should be noted that variations in temperature in furrow, prevents direct sunlight.
The major defect of said method is: yield is unstable, deficiency in economic performance;It is difficult on a large scale in High aititude region Morchella esculenta (L.) Pers artificial culture;Lack a complete set of technical solution, cause the cultivation of heliogreenhouse Morchella esculenta (L.) Pers there is no successful precedent.
Summary of the invention
The technical problem to be solved is to provide a kind of Morchella esculenta (L.) Pers heliogreenhouse simple to operate, easy to spread and imitates Habitat high yield cultivating method.
For solving the problems referred to above, a kind of bionical border of Morchella esculenta (L.) Pers heliogreenhouse of the present invention high yield cultivating method, including Following steps:
(1) prepare fluid medium:
By peeling potatoes, washing, stripping and slicing, add water boil 15 ~ 20 minutes, take double gauze after smashing to pieces and filter, obtain filtrate;Institute State and filtrate adds the glucose of its quality 3%, the Semen Maydis powder of 1%, the magnesium sulfate of 0.05%, the potassium dihydrogen phosphate of 0.1%, mixed After closing uniformly, being sub-packed in while hot in 500mL triangular flask by the loading amount of every bottle of 100mL and seal, under 0.1MPa pressure, high pressure goes out Bacterium 30min, obtains fluid medium, and this liquid culture is stand-by based on preserving in gnotobasis;
(2) robust growth, individual Morchella esculenta (L.) Pers sporophore big, that growing way is good in wild environment are removed stem, remaining cap volume After concentration is the ethanol surface sterilization of 70%, through aseptic water washing and after wiping surface moisture with sterile gauze, cut 5mm × 5mm Bacterial context 2 ~ 3 pieces, be inoculated in fluid medium described in 100mL, prior to culturing room's A quiescent culture 24 hours, then through reciprocating On shaking table, shaken cultivation 10 ~ 20 days, obtain mother culture media;
(3) prepare Cultivar culture medium:
By weight percentage, by Fructus Mali pumilae wood flour 70 ~ 80%, wheat grain 15 ~ 20%, soil 3 ~ 7%, Calx 0.5 ~ 1.5%, Gypsum Fibrosum 0.5 ~ After the raw material mixing of 1.5%, add water and stir, obtain the compost that water content is 60 ~ 70%;Described compost loads diameter 10cm, length 30cm polyethylene plastic bag in, and make charge weight account for sack 2/3, bag mouth fills after tightening under 0.15MPa pressure Pot sterilizing 1.5 ~ 2h, obtains Cultivar culture medium;
(4) the transfer room after cleaning-sterilizing carries out purple to described mother culture media, Cultivar culture medium, inoculating tool respectively Outside line lamp sterilization, meanwhile, the alcohol disinfecting that bottleneck volumetric concentration is 70% to the described mother culture media of loading, then Extract described mother culture media with Inoculating needle, inject described in 5mL in Cultivar culture medium every bag described by alcohol burner flame Mother culture media is inoculated;
(5) inoculate complete, the cultivating bag dislocation culturing room B of described step (4) gained is sent out bacterium, after 15 days, i.e. obtains white hypha;
(6) select large span, heliogreenhouse without column, and uniformly apply Calx 80 ~ 100kg by every mu of sown area, make soil PH value is 7 ~ 9, simultaneously, it is ensured that making soil moisture is 30 ~ 40%;
(7) bedder-planter:
Select the first tenday period of a month in January, the horizontal ridging of wall East and West direction after the heliogreenhouse after 800 times of liquid spraying disinfections of carbendazim, with Time, by every mu of 40 ~ 50kg strain amount, described white hypha is uniformly sprinkling upon on face, ridge, and the soil of furrow is overlying on has spread strain Face, ridge, cladding thickness is 3 ~ 5cm;After planting opening sprinkling irrigation regulation soil moisture, soil moisture content reaches 60 ~ 70%;
(8) hair tube reason:
1. mycelial growth prophase management:
In 2 months after planting, it is ensured that at the 5cm of underground, the soil moisture is between 5 ~-10 DEG C, indoor temperature between 8 ~-16 DEG C, Soil moisture content is 60 ~ 70%;On 1.8 ~ 2 meters of sunlight indoor distances ground, eminence horizontally suspends black 6 pin sunshade net so that it is Sunshade rate >=70%;Ventilate noon every day, every time no less than 2 hours simultaneously;
2. mycelial growth final-period management:
Entering mid-March, control sunlight indoor temperature is between 8 ~ 18 DEG C, and relative moisture of the soil is maintained at 70% ~ 80%, air Humidity 50% ~ 70%, and by the every ridge of the trend on ridge put two rows towards soil one side punching and filling water content be 60 ~ 70% inclusions Nutrient bag, often row nutrient bag between be spaced 50 ~ 70cm;After described nutrient bag puts one month, when Morchella esculenta (L.) Pers sporophore is formed Progressively remove after 80%;
3. sporophore growth period management:
When having small particles to be formed on mycelia seen from naked eyes on face, ridge, enter sporophore management phase, now control heliogreenhouse Interior temperature is between 8 ~ 18 DEG C, and soil moisture is maintained at 60 ~ 70%, and air humidity 45 ~ 55%, simultaneously with 800 times of liquid of 50% carbendazim Spraying disinfection on heliogreenhouse passageway and wall;
(9) gather: Morchella esculenta (L.) Pers cap length to 4~7cm, just can gather during the gradually diastole of the fold on cap.
The temperature of described step ⑵Zhong culturing room A is 16 DEG C ~ 18 DEG C.
On described step (2) middle reciprocal shaker, the condition of shaken cultivation refers to that frequency of oscillation is 80 times per minute, and amplitude is 6~10cm。
The temperature of described step ⑸Zhong culturing room B is 8 DEG C ~ 18 DEG C, relative air humidity≤65%.
Described step (7) middle ridging refers to row spacing 1.5m, furrow width 30cm, deep 20cm.
The hole beaten on described step 2. Middle nutrition bag is 5 ~ 6, and each aperture is 1 ~ 1.5cm.
Inclusions in described step 2. Middle nutrition bag refers to by weight percentage, by Fructus Mali pumilae wood flour 70 ~ 80%, wheat grain 15 ~ 20%, after the raw material mixing of soil 3 ~ 7%, Calx 0.5 ~ 1.5%, Gypsum Fibrosum 0.5 ~ 1.5%, add water and stir and get final product.
The present invention compared with prior art has the advantage that
1, the present invention is by making mother's kind, cultigen after the most local wild toadstool of collection, then carries out in heliogreenhouse Send out bacterium, carry out whole process by intensity of illumination, air flux, soil pH value, soil temperature and humidity, aerial temperature and humidity etc. are sent out collarium border Bionical border is simulated, thus realizes high-yield high-efficiency cultivation.
2, the present invention is simple to operate, it is easy to large-scale demonstration is promoted, and products obtained therefrom quality better.
Detailed description of the invention
A kind of Morchella esculenta (L.) Pers heliogreenhouse bionical border high yield cultivating method, comprises the following steps:
(1) prepare fluid medium:
By peeling potatoes, washing, stripping and slicing, add water boil 15 ~ 20 minutes, take double gauze after smashing to pieces and filter, obtain filtrate;Filter Adding the glucose of its quality 3%, the Semen Maydis powder of 1%, the magnesium sulfate of 0.05%, the potassium dihydrogen phosphate of 0.1% in liquid, mixing is all After even, it be sub-packed in while hot in 500mL triangular flask by the loading amount of every bottle of 100mL and seal, autoclaving under 0.1MPa pressure 30min, obtains fluid medium, and this liquid culture is stand-by based on preserving in gnotobasis.
(2) use tissue isolation to carry out female kind to separate.By robust growth in wild environment, individual big, Morchella esculenta (L.) Pers that growing way is good Sporophore removes stem, after remaining cap volumetric concentration is the ethanol surface sterilization of 70%, through aseptic water washing and with aseptic After surface moisture wiped by gauze, cut the bacterial context 2 ~ 3 pieces of 5mm × 5mm, be inoculated in 100mL fluid medium, prior to culturing room A quiescent culture 24 hours, then shaken cultivation 10 ~ 20 days on reciprocal shaker, obtain mother culture media.
Wherein: the temperature of culturing room A is 16 DEG C ~ 18 DEG C.On reciprocal shaker, the condition of shaken cultivation refers to frequency of oscillation For 80 times per minute, amplitude is 6 ~ 10cm.
(3) prepare Cultivar culture medium:
By weight percentage, by Fructus Mali pumilae wood flour 70 ~ 80%, wheat grain 15 ~ 20%, soil 3 ~ 7%, Calx 0.5 ~ 1.5%, Gypsum Fibrosum 0.5 ~ After the raw material mixing of 1.5%, add water and stir, obtain the compost that water content is 60 ~ 70%;Compost loading diameter 10cm, In the polyethylene plastic bag of length 30cm, and making charge weight account for sack 2/3, bag mouth fills pot under 0.15MPa pressure after tightening and goes out Bacterium 1.5 ~ 2h, obtains Cultivar culture medium.
(4) the transfer room after cleaning-sterilizing carries out purple to mother culture media, Cultivar culture medium, inoculating tool respectively Outside line lamp sterilization, meanwhile, the alcohol disinfecting that bottleneck volumetric concentration is 70% to loading mother culture media, then with connecing Plant pin extraction mother culture media, by alcohol burner flame, in every bag cultivating kind culture medium, inject 5mL mother culture media connect Kind.
(5) inoculating complete, the cultivating bag dislocation culturing room B of step (4) gained is sent out bacterium, the temperature of culturing room B is 8 DEG C ~ 18 ℃.Note ventilation, in order to avoid indoor carbon dioxide excessive concentration affects mycelial growth.Indoor holding cleaning, air is the wettest Degree≤65%.White hypha is i.e. obtained after 15 days.Make regular check on, clear up the bacterium bag being bacterial contamination in time.
(6) select large span, heliogreenhouse without column, and uniformly apply Calx 80 ~ 100kg by every mu of sown area, make Soil pH value is 7 ~ 9, simultaneously, it is ensured that making soil moisture is 30 ~ 40%.
(7) bedder-planter:
Select the first tenday period of a month in January, the horizontal ridging of wall East and West direction after the heliogreenhouse after 800 times of liquid spraying disinfections of carbendazim, row spacing 1.5m, furrow width 30cm, deep 20cm., by every mu of 40 ~ 50kg strain amount, white hypha is uniformly sprinkling upon on face, ridge meanwhile, and will The soil of furrow is overlying on the face, ridge having spread strain, and cladding thickness is 3 ~ 5cm.Keep soil can thaw during sowing on freeze-up daytime in the evening Shi Weiyi, after planting opens sprinkling irrigation regulation soil moisture, and soil moisture content reaches 60 ~ 70%.
(8) hair tube reason:
1. mycelial growth prophase management:
In 2 months after planting, night, temperature indoor temperature became with outdoor temperature change without putting insulation quilt or insulation straw screen or mat Change, it is ensured that at the 5cm of underground, the soil moisture is between 5 ~-10 DEG C, and indoor temperature is between 8 ~-16 DEG C.Morchella esculenta (L.) Pers happiness is wet, and soil contains The water yield is 60 ~ 70%, and less than 60%, water spray improves humidity in time.Morchella esculenta (L.) Pers growth needs scattered light, on sunlight indoor distances ground 1.8 ~ 2 meters of face eminence horizontally suspends black 6 pin sunshade net so that it is sunshade rate >=70%;Ventilate, the most much noon every day simultaneously In 2 hours.
2. mycelial growth final-period management:
Enter mid-March, by regulation and control air port with take off and put front roof bottom canopy film, control sunlight indoor temperature 8 ~ 18 DEG C it Between, simultaneously work as adding forced ventilation, to stimulate the effect of the formation of Morchella esculenta (L.) Pers sporophore.Relative moisture of the soil is maintained at 70% ~ 80%, Air humidity 50% ~ 70%, humidity is sprayed water the most at any time and is supplemented.When mycelia is flourished, when in canopy, soil is far seen and turned white, in order to give Mycelial growth provides sufficient nutrient, by the every ridge of the trend on ridge put two rows towards soil one side punching and filling water content be 60 ~ The nutrient bag of 70% inclusions, is often spaced 50 ~ 70cm between row's nutrient bag;After nutrient bag puts one month, work as Morchella esculenta (L.) Pers sporophore Progressively remove after forming 80%.
Wherein: the hole beaten on nutrient bag is 5 ~ 6, each aperture is 1 ~ 1.5cm.
Inclusions in nutrient bag refers to by weight percentage, by Fructus Mali pumilae wood flour 70 ~ 80%, wheat grain 15 ~ 20%, soil 3 ~ 7%, after the raw material mixing of Calx 0.5 ~ 1.5%, Gypsum Fibrosum 0.5 ~ 1.5%, add water and stir and get final product.
3. sporophore growth period management:
When having small particles to be formed on mycelia seen from naked eyes on face, ridge, enter sporophore management phase, now control heliogreenhouse Interior temperature, between 8 ~ 18 DEG C, adds forced ventilation to prevent indoor CO2Accumulation, affects sporophore growth.General fine day sprays water 1 every day ~ 2 times, every 3 ~ 5 days of cloudy day sprayed water 1 time, and soil moisture is maintained at 60 ~ 70%, and air humidity 45 ~ 55% notes utilizing sunshade net to hide The scattered light environment that cloudy formation is faint, definitely avoids strong face, sunlight direct projection ridge.For preventing living contaminants, simultaneously with 50% Carbendazim 800 times of liquid spraying disinfection on heliogreenhouse passageway and wall.
(9) gather: Morchella esculenta (L.) Pers cap length to 4~7cm, just can gather during the gradually diastole of the fold on cap.When gathering Cutting off gently from stem base portion with sharp blade, attention action wants light, prevents sporophore damage little near causing, and impact is total Body yield.

Claims (7)

1. a Morchella esculenta (L.) Pers heliogreenhouse bionical border high yield cultivating method, comprises the following steps:
(1) prepare fluid medium:
By peeling potatoes, washing, stripping and slicing, add water boil 15 ~ 20 minutes, take double gauze after smashing to pieces and filter, obtain filtrate;Institute State and filtrate adds the glucose of its quality 3%, the Semen Maydis powder of 1%, the magnesium sulfate of 0.05%, the potassium dihydrogen phosphate of 0.1%, mixed After closing uniformly, being sub-packed in while hot in 500mL triangular flask by the loading amount of every bottle of 100mL and seal, under 0.1MPa pressure, high pressure goes out Bacterium 30min, obtains fluid medium, and this liquid culture is stand-by based on preserving in gnotobasis;
(2) robust growth, individual Morchella esculenta (L.) Pers sporophore big, that growing way is good in wild environment are removed stem, remaining cap volume After concentration is the ethanol surface sterilization of 70%, through aseptic water washing and after wiping surface moisture with sterile gauze, cut 5mm × 5mm Bacterial context 2 ~ 3 pieces, be inoculated in fluid medium described in 100mL, prior to culturing room's A quiescent culture 24 hours, then through reciprocating On shaking table, shaken cultivation 10 ~ 20 days, obtain mother culture media;
(3) prepare Cultivar culture medium:
By weight percentage, by Fructus Mali pumilae wood flour 70 ~ 80%, wheat grain 15 ~ 20%, soil 3 ~ 7%, Calx 0.5 ~ 1.5%, Gypsum Fibrosum 0.5 ~ After the raw material mixing of 1.5%, add water and stir, obtain the compost that water content is 60 ~ 70%;Described compost loads diameter 10cm, length 30cm polyethylene plastic bag in, and make charge weight account for sack 2/3, bag mouth fills after tightening under 0.15MPa pressure Pot sterilizing 1.5 ~ 2h, obtains Cultivar culture medium;
(4) the transfer room after cleaning-sterilizing carries out purple to described mother culture media, Cultivar culture medium, inoculating tool respectively Outside line lamp sterilization, meanwhile, the alcohol disinfecting that bottleneck volumetric concentration is 70% to the described mother culture media of loading, then Extract described mother culture media with Inoculating needle, inject described in 5mL in Cultivar culture medium every bag described by alcohol burner flame Mother culture media is inoculated;
(5) inoculate complete, the cultivating bag dislocation culturing room B of described step (4) gained is sent out bacterium, after 15 days, i.e. obtains white hypha;
(6) select large span, heliogreenhouse without column, and uniformly apply Calx 80 ~ 100kg by every mu of sown area, make soil PH value is 7 ~ 9, simultaneously, it is ensured that making soil moisture is 30 ~ 40%;
(7) bedder-planter:
Select the first tenday period of a month in January, the horizontal ridging of wall East and West direction after the heliogreenhouse after 800 times of liquid spraying disinfections of carbendazim, with Time, by every mu of 40 ~ 50kg strain amount, described white hypha is uniformly sprinkling upon on face, ridge, and the soil of furrow is overlying on has spread strain Face, ridge, cladding thickness is 3 ~ 5cm;After planting opening sprinkling irrigation regulation soil moisture, soil moisture content reaches 60 ~ 70%;
(8) hair tube reason:
1. mycelial growth prophase management:
In 2 months after planting, it is ensured that at the 5cm of underground, the soil moisture is between 5 ~-10 DEG C, indoor temperature between 8 ~-16 DEG C, Soil moisture content is 60 ~ 70%;On 1.8 ~ 2 meters of sunlight indoor distances ground, eminence horizontally suspends black 6 pin sunshade net so that it is Sunshade rate >=70%;Ventilate noon every day, every time no less than 2 hours simultaneously;
2. mycelial growth final-period management:
Entering mid-March, control sunlight indoor temperature is between 8 ~ 18 DEG C, and relative moisture of the soil is maintained at 70% ~ 80%, air Humidity 50% ~ 70%, and by the every ridge of the trend on ridge put two rows towards soil one side punching and filling water content be 60 ~ 70% inclusions Nutrient bag, often row nutrient bag between be spaced 50 ~ 70cm;After described nutrient bag puts one month, when Morchella esculenta (L.) Pers sporophore is formed Progressively remove after 80%;
3. sporophore growth period management:
When having small particles to be formed on mycelia seen from naked eyes on face, ridge, enter sporophore management phase, now control heliogreenhouse Interior temperature is between 8 ~ 18 DEG C, and soil moisture is maintained at 60 ~ 70%, and air humidity 45 ~ 55%, simultaneously with 800 times of liquid of 50% carbendazim Spraying disinfection on heliogreenhouse passageway and wall;
(9) gather: Morchella esculenta (L.) Pers cap length to 4~7cm, just can gather during the gradually diastole of the fold on cap.
2. a kind of bionical border of Morchella esculenta (L.) Pers heliogreenhouse as claimed in claim 1 high yield cultivating method, it is characterised in that: described step The temperature of Zhou⑵Zhong culturing room A is 16 DEG C ~ 18 DEG C.
3. a kind of bionical border of Morchella esculenta (L.) Pers heliogreenhouse as claimed in claim 1 high yield cultivating method, it is characterised in that: described step On rapid (2) middle reciprocal shaker, the condition of shaken cultivation refers to that frequency of oscillation is 80 times per minute, and amplitude is 6 ~ 10cm.
4. a kind of bionical border of Morchella esculenta (L.) Pers heliogreenhouse as claimed in claim 1 high yield cultivating method, it is characterised in that: described step The temperature of Zhou⑸Zhong culturing room B is 8 DEG C ~ 18 DEG C, relative air humidity≤65%.
5. a kind of bionical border of Morchella esculenta (L.) Pers heliogreenhouse as claimed in claim 1 high yield cultivating method, it is characterised in that: described step Rapid (7) middle ridging refers to row spacing 1.5m, furrow width 30cm, deep 20cm.
6. a kind of bionical border of Morchella esculenta (L.) Pers heliogreenhouse as claimed in claim 1 high yield cultivating method, it is characterised in that: described step The hole beaten on rapid 2. Middle nutrition bag is 5 ~ 6, and each aperture is 1 ~ 1.5cm.
7. a kind of bionical border of Morchella esculenta (L.) Pers heliogreenhouse as claimed in claim 1 high yield cultivating method, it is characterised in that: described step Inclusions in rapid the most 2. Middle nutrition bag refers to by weight percentage, by Fructus Mali pumilae wood flour 70 ~ 80%, wheat grain 15 ~ 20%, soil 3 ~ 7%, after the raw material mixing of Calx 0.5 ~ 1.5%, Gypsum Fibrosum 0.5 ~ 1.5%, add water and stir and get final product.
CN201610646048.9A 2016-08-09 2016-08-09 Morchella sunlight greenhouse bionic environment high-yield cultivation method Pending CN106305131A (en)

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CN108522153A (en) * 2018-04-25 2018-09-14 北京市农业技术推广站 A method of utilizing liquid spawn fast-propagation hickory chick cultigen
CN108522139A (en) * 2018-04-25 2018-09-14 赵金亮 A kind of efficient implantation methods of brooder hickory chick
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CN110771425A (en) * 2019-09-29 2020-02-11 青海大学 Factory three-dimensional cultivation method for morchella
CN113455284A (en) * 2021-08-15 2021-10-01 宁夏农林科学院园艺研究所(宁夏设施农业工程技术研究中心) Method for manufacturing exogenous nutrition bag for improving yield and quality of morchella esculenta

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CN106818215A (en) * 2017-02-24 2017-06-13 广东东阳光药业有限公司 Terraced rib hickory chick industrial planting method
CN107509529A (en) * 2017-08-23 2017-12-26 佛山推启农业研究院(普通合伙) A kind of artificial cultivation method for improving hickory chick amino acid content
CN107573127A (en) * 2017-10-12 2018-01-12 山西林业职业技术学院 A kind of new hickory chick foreign aid nutrient bag alternative
CN107593275A (en) * 2017-11-13 2018-01-19 云南菌视界生物科技有限公司 A kind of method of photovoltaic green-house cultivation hickory chick
CN108522153A (en) * 2018-04-25 2018-09-14 北京市农业技术推广站 A method of utilizing liquid spawn fast-propagation hickory chick cultigen
CN108522139A (en) * 2018-04-25 2018-09-14 赵金亮 A kind of efficient implantation methods of brooder hickory chick
CN109496688A (en) * 2018-11-20 2019-03-22 辽宁农业职业技术学院 A kind of hickory chick industrial culture method
CN109997605A (en) * 2019-05-09 2019-07-12 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) A kind of hickory chick box type stereo efficient cultivation method
CN110771425A (en) * 2019-09-29 2020-02-11 青海大学 Factory three-dimensional cultivation method for morchella
CN113455284A (en) * 2021-08-15 2021-10-01 宁夏农林科学院园艺研究所(宁夏设施农业工程技术研究中心) Method for manufacturing exogenous nutrition bag for improving yield and quality of morchella esculenta

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Application publication date: 20170111