CN106818215A - Terraced rib hickory chick industrial planting method - Google Patents
Terraced rib hickory chick industrial planting method Download PDFInfo
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- CN106818215A CN106818215A CN201710102760.7A CN201710102760A CN106818215A CN 106818215 A CN106818215 A CN 106818215A CN 201710102760 A CN201710102760 A CN 201710102760A CN 106818215 A CN106818215 A CN 106818215A
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- 239000011159 matrix material Substances 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 238000005286 illumination Methods 0.000 claims description 32
- 238000003306 harvesting Methods 0.000 claims description 27
- 238000012423 maintenance Methods 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 13
- 239000002609 medium Substances 0.000 claims description 13
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- 241000894006 Bacteria Species 0.000 claims description 11
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 150000001875 compounds Chemical class 0.000 claims description 7
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 5
- 244000105624 Arachis hypogaea Species 0.000 claims description 5
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 5
- 235000018262 Arachis monticola Nutrition 0.000 claims description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 5
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 239000010903 husk Substances 0.000 claims description 5
- 210000004185 liver Anatomy 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 235000009973 maize Nutrition 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- 235000020232 peanut Nutrition 0.000 claims description 5
- 239000003415 peat Substances 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 230000000737 periodic effect Effects 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000010902 straw Substances 0.000 claims description 5
- 241001446247 uncultured actinomycete Species 0.000 claims description 5
- 239000010455 vermiculite Substances 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 abstract description 3
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- 230000000638 stimulation Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 238000012549 training Methods 0.000 description 5
- 240000002769 Morchella esculenta Species 0.000 description 4
- 235000002779 Morchella esculenta Nutrition 0.000 description 4
- 238000012364 cultivation method Methods 0.000 description 4
- 239000008057 potassium phosphate buffer Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 241000221638 Morchella Species 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 240000004875 Carya cathayensis Species 0.000 description 1
- 235000005663 Carya cathayensis Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- 241000221637 Morchellaceae Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000287127 Passeridae Species 0.000 description 1
- 241000221700 Pezizales Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
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- 238000011084 recovery Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
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- 230000001256 tonic effect Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- General Health & Medical Sciences (AREA)
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- Mushroom Cultivation (AREA)
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Abstract
The invention provides a kind of terraced rib hickory chick industrial planting method, it is characterised in that comprise the following steps：(1) cultivation matrix is prepared；(2) liquid spawn makes；(3) cultural hypha；(4) management of producing mushroom：Step (3) described mycelium is transferred in mushroom producing room carries out management of producing mushroom, is divided into promotion former base and forms phase, mushroom flower bud formation phase, growth period and ripe period management.The method is used by raw material of wastes of crops and prepares cultivation matrix, sowed using liquid spawn, hickory chick factory culture is fully achieved indoors, simple to operate, low cost, cycle is short, quality are controllable, can obtain that a kind of pollution-free, delicate fragrance is pleasant, food appetizing green product throughout the year in factorial praluction.
Description
Technical field
The present invention relates to field of edible fungus culture, and in particular in a kind of terraced rib hickory chick room or industrial planting method.
Technical background
Hickory chick (Morchella) belongs to discomycete also known as sheep tripe mushroom, sheep tripe dish, sheep belly, positive sparrow bacterium etc., Pezizale,
Morchellaceae, morchella was found in 1818, and because its mushroom cap surface is uneven, form exactly likes sheep tripe and gains the name.Sheep tripe
Bacterium is rare edible and medical fungi, and delicious flavour is nutritious, there is high nutrition and medical value, is both the treasure in feast
Product, are again the tonic non-defective units having long enjoyed a good reputation, with enhancing immunity of organisms, antifatigue, antiviral, kidney tonifying, establishing-Yang the effects such as.It is wild
Raw collection is the mode that tradition obtains hickory chick, is influenceed by natural environment and climate change, and wild resource is reduced year by year, and market is not for
Should ask, it is expensive so that artificial cultivation turns into inevitable.
In very long heuristic process, the stability problem of yield and quality is always its nothing to hickory chick artificial cultivation technique
The problem that method is captured.Abroad, only has the factory culture that DNP companies of the U.S. tentatively realize hickory chick in the world at present.
DNP companies in 2005 have built huge Edible Fungi factory in Michigan, USA, and 3000 pounds of hickory chicks are produced weekly.
The hickory chick production of DNP companies in 2008 encounters the unstable technical barrier of yield, and cannot solve always and stop production.Cause
This, merchandized handling Morchella esculenta (L.) Pers sporophore not yet making a breakthrough property achievement so far.
At home, Chinese hickory chick field field production has been carried out scale, commercial growth, but is also subject to nature
Condition and local climate limitation influence and there is failure and the influence of low yield.Current Duo Jia research institutions successfully realize sheep
Tripe bacterium crop field is manually commercialized cultivation, is up to nearly 6 months in outdoor crop cycle, and one is only a season, and the Nature conditional instability
And uncertain factor is numerous, often lead to some times receives or has no harvest less, causes Planting household huge economic losses.Interior is carried out
Factory culture turns into the technical barrier for being badly in need of solving.The patent of invention of Zhu Douxi professors《Indoor cultivation method of morel》(specially
Sharp application number 200610076953.1) and《Hickory chick industrial production method》In (number of patent application 201410029430.9) only
Mention yield high, but be not directed to specific yield, and the latter mentions being burrowed in charge level and sows seeds, process is relatively complicated, time-consuming.Mesh
Preceding domestic hickory chick indoor growing fruiting there is no real breakthrough, and one of key reason is management of producing mushroom.
The content of the invention
The present inventor in view of the shortcomings of the prior art, by terraced rib hickory chick indoor growing technique research and development, before summary
On the basis of people's open air plantation successful experience, with the Nature as research object, imitated by indoor growing environment indices
Ecological regulation and control, and the strict manipulation to terraced rib hickory chick management of producing mushroom, successfully realize the indoor growing of hickory chick finally
Fruiting, obtains good yield, while indoor growing environmental condition is artificially controllable, can steadily in the long term run production, favorably
Amplify in scale chemical plant, and the invention has the features such as low, yield of investment is high, quality is good, raw material sources are abundant.
The invention provides a kind of batch production of terraced rib hickory chick or indoor cultivation method for Ganoderma, the method is used with wastes of crops
For raw material prepares cultivation matrix, sowed using liquid spawn, hickory chick factory culture, operation letter are fully achieved indoors
List, low cost, cycle is short, quality are controllable, can obtain that a kind of pollution-free, delicate fragrance is pleasant, food U.S. throughout the year in factorial praluction
The good to eat green product of taste.
The present invention is to be achieved through the following technical solutions：
The invention provides a kind of terraced rib hickory chick industrial planting method, it is characterised in that comprise the following steps：
(1) cultivation matrix is prepared：Mainly it is made up of the raw material of following parts by weight：Peat soil 60-70 parts, fertile soil 5-10
Part, cow dung 3-5 parts, vermiculite 3-5 parts, liver moss 5-10 parts, husk 5-10 parts, maize straw 5-8 parts, peanut shell 3-5 parts, soybean peel
3-5 parts；
(2) liquid spawn makes：The parent species of the inclined-plane culture that will have been activated are linked into fluid nutrient medium in an aseptic environment
In carry out shaking table culture, shaking table culture obtains cultigen after 3-5 days；
(3) cultural hypha：Step (2) gained cultigen is uniformly sprinkling upon on the cultivation matrix obtained by step (1), is then covered
Described in lid 2-3cm after cultivation matrix in culturing room lucifuge culture 25-35 days；
(4) management of producing mushroom：Step (3) described mycelium is transferred in mushroom producing room carries out management of producing mushroom, is divided into promotion former
Base forms phase, mushroom flower bud formation phase, growth period and ripe period management：
Former base is promoted to form the phase：The cultivation matrix humidity is kept for 70-80%, daily illumination 6-8h, intensity of illumination is
100-200 1x, control 10-22 DEG C of temperature；
Mushroom flower bud forms the phase：Keep the cultivation matrix humidity for 60-70%, daily illumination 8-12h, intensity of illumination is 200-
500lx, controls 4-18 DEG C of temperature；
Growth period and maturity period：It is 80-90% to keep cultivation matrix humidity, daily illumination 12-20h, and intensity of illumination is
500-800lx, keeps 22 DEG C of steady temperature；
Gas concentration lwevel is 0.3-0.4% during management of producing mushroom keeps mushroom producing room, and management of producing mushroom harvests ripe sheep for 35-45 days
Tripe mushroom entity.
In certain embodiments, the raw material composition described in blend step (1), after stirring, sprinkling addition contains brown
The pH value of thermophilic actinomycete and bacillus subtilis is 7.5-8.0 potassium phosphate buffer solutions so that moisture reaches in compound
To 50-60%, after being well mixed, stack 3-4 days, that is, obtain cultivation matrix.
In certain embodiments, the fluid nutrient medium described in step (2) is mainly made up of following components：Glucose 30-
40g/L, yeast extract 20-30g/L, peptone 20-30g/L, potassium dihydrogen phosphate 3-5g/L, magnesium sulfate 3-5g/L.
In certain embodiments, shaking table culture condition is in step (2)：According to the weight hundred of parent species amount and fluid nutrient medium
Divide than for be linked into parent species in fluid nutrient medium by 2-3%, being then placed within being cultivated on constant-temperature table, shaking table temperature is 22-24
DEG C, shaking speed is 100-120r/min.
In certain embodiments, culture room temperature is 10-16 DEG C in step (3), and the cultivation matrix humidity is 50-
65%.
In certain embodiments, in step (4)：
Promotion former base forms the temperature control of phase：16-22 DEG C of maintenance 10-16h of control temperature, controls temperature 10- daily
15 DEG C of maintenance 8-14h；
Mushroom flower bud formed the phase temperature control be：11-18 DEG C of maintenance 14-18h of control temperature, controls 4-10 DEG C of guarantor of temperature daily
Hold 6-10h.
In certain embodiments, further include to harvest step, it is accurate during stem 4-6cm long as bacteria cover diameter 3.5-6.5cm
Standby harvesting, and 3-5 days before harvesting, cultivation matrix humidity is controlled for 60-70%, periodic Control is harvested at 5-10 days, after harvesting
Mushroom producing room is thoroughly cleaned, is sterilized in time.
The morchella mother culture that the method for the invention is used is to utilize this area conventional technical means from wild toadstool
Separate and obtain, the hickory chick strain being inoculated with can be obtained by the culture of the art conventional method.
Artificial lighting used by the method for the invention can be obtained by daylight lamp source or LED lamp source.
Compared with prior art, beneficial effects of the present invention are：
(1) liquid spawn that the present invention is provided makes simple, the making of solid spawn to pass through from parent species to original seed again to
Cultigen process, generally requires 3 months or so, and needs to change culture medium.Liquid spawn typically only needs 3-5 days or so, and
Inoculation is convenient, and mycelia can develop rapidly after inoculation, and mycelium pellet quantity is more, be evenly distributed.
(2) the management of producing mushroom technology that the present invention is provided borrows modern project technology and sophisticated equipment, sets terraced rib hickory chick
Condition and the constant gas concentration lwevels such as steps temperature, humidity, intensity of illumination required for growing, it is ensured that terraced rib
Hickory chick promote former base formed phase, mushroom flower bud phase, growth period and nutriment required for each stage in maturity period, temperature,
Illumination, suitably gives thermal stimulation, light stimulation and warm and humid stimulation, and then promote the formation of hickory chick former base and later stage quickly to send out
Morchella esculenta (L.) Pers sporophore is bred as, the whole technological process of production, production technical standard, product quality equalization substantially reduces life
Long period.
(3) the terraced rib indoor cultivation method of morel that the present invention is provided fully achieves annual roller production, overcomes
Traditional cultivation method cultivates the limitation in one to two seasons for 1 year, and production process is not restricted by natural environment, is increased substantially
Labor productivity, qualified product, pollution is few, and low cost is greatly facilitated hickory chick stable high yield, can ensure that now and often put down
The annual production of square rice hickory chick reaches 1.5-1.8kg.
Specific embodiment
For the ease of understanding the present invention, especially exemplified by following examples, its effect should be construed as to elaboration of the invention
Rather than to any type of limitation of the invention.It should be noted that for a person skilled in the art, in this innovation and creation
On the basis of, any deformation and improvement made belong to protection scope of the present invention, and all raw materials can lead in embodiment
Cross commercial sources acquisition.For a further understanding of the present invention, with reference to a kind of indoor growing that embodiment is provided the present invention
The method of hickory chick is described in detail.
Embodiment 1
The indoor growing or industrial planting method of a kind of terraced rib hickory chick, comprise the following steps：
1) substrate preparation：Weigh the raw material of following parts by weight：60 parts of peat soil, 5 parts of fertile soil, 3 parts of cow dung, vermiculite 3
Part, 5 parts of liver moss, 5 parts of husk, 5 parts of maize straw, 3 parts of peanut shell, 3 parts of soybean peel；Mixing, is stirring evenly and then adding into containing brown
The pH of color thermophilic actinomycete and bacillus subtilis is 7.5 potassium phosphate buffer solution sprinkling compound so that mixture moisture
Content reaches 50%, after being well mixed, stacks 3 days, and daily turning once, that is, obtains cultivation matrix；
2) liquid spawn makes：The parent species of the inclined-plane culture that will have been activated in advance are in an aseptic environment according to parent species amount and liquid
The percentage by weight of body culture medium is linked into fluid nutrient medium for 2%, is then placed within being cultivated on constant-temperature table, shaking table temperature
22-24 DEG C is controlled to, shaking speed is 100r/min, shaking table culture 3 days.Wherein, fluid nutrient medium is made up of following components：Portugal
Grape sugar 30g/L, yeast extract 20g/L, peptone 20g/L, potassium dihydrogen phosphate 3g/L, magnesium sulfate 3g/L；
3) cultural hypha：Step (2) gained liquid culture bacteria is uniformly sprinkling upon on the cultivation matrix obtained by step (1),
Then culture is started after covering 2-3cm cultivation matrixes again, it is 10 DEG C to control culture room temperature, and cultivation matrix humidity is 50%, is kept away
Optical culture 35 days；
4) management of producing mushroom：Step (3) mycelium is transferred to temperature in 16 DEG C of mushroom producing rooms, to keep mushroom producing room carbon dioxide
Concentration is 0.3%, management of producing mushroom 35 days.Wherein, mycelium management of producing mushroom in mushroom producing room is formed for former base forms phase, mushroom flower bud
Phase, growth period and maturity period.Former base is promoted to form the phase：It is 70%, daily illumination 6h, intensity of illumination to keep cultivation matrix humidity
It is 200 1x, 16 DEG C of maintenance 10h of control temperature, control 10 DEG C of maintenance 14h of temperature daily；Mushroom flower bud forms the phase：Keep cultivation matrix
Humidity is 60%, daily illumination 8h, and intensity of illumination is 500lx, daily 11 DEG C of maintenance 14h of control temperature, the 4 DEG C of maintenances of control temperature
10h；Growth period and maturity period：It is 80%, daily illumination 12h to keep cultivation matrix humidity, and intensity of illumination is 800lx, keeps permanent
22 DEG C of constant temperature degree；
5) harvest：As bacteria cover diameter 3.5-6.5cm, harvesting is prepared during stem 4-6cm long, and 3 days before harvesting, control is planted
Training soil humidity is 60%, and harvesting periodic Control was thoroughly cleaned to mushroom producing room, sterilized in time at 5 days after harvesting.Through plucking
Result data is counted, and is produced per year for every square metre and is reached about 1.6kg.
Embodiment 2
The indoor growing or industrial planting method of a kind of terraced rib hickory chick, comprise the following steps：
1) substrate preparation：Weigh the raw material of following parts by weight：65 parts of peat soil, 7 parts of fertile soil, 4 parts of cow dung, vermiculite 4
Part, 7 parts of liver moss, 7 parts of husk, 7 parts of maize straw, 4 parts of peanut shell, 4 parts of soybean peel；Mixing, is stirring evenly and then adding into containing brown
The pH value of color thermophilic actinomycete and bacillus subtilis is 7.7 potassium phosphate buffer solution sprinkling compound so that compound water
Point content reaches 55%, after being well mixed, stacks 4 days, and daily turning once, that is, obtains cultivation matrix；
2) liquid spawn makes：The parent species of the inclined-plane culture that will have been activated in advance are in an aseptic environment according to parent species amount：Liquid
The percentage by weight of body culture medium is linked into fluid nutrient medium for 2.5%, is then placed within being cultivated on constant-temperature table, shaking table temperature
Degree is controlled to 22-24 DEG C, and shaking speed is 110r/min, shaking table culture 4 days.Wherein, fluid nutrient medium is made up of following components：
Glucose 35g/L, yeast extract 25g/L, peptone 25g/L, potassium dihydrogen phosphate 4g/L, magnesium sulfate 4g/L；
3) cultural hypha：Step (2) gained liquid culture bacteria is uniformly sprinkling upon on the cultivation matrix of step (1), then
Start culture after covering 2-3cm cultivation matrixes again, it is 14 DEG C to control culture room temperature, and cultivation matrix humidity is 58%, lucifuge training
Support 30 days；
4) management of producing mushroom：Step (4) mycelium is transferred to temperature in 19 DEG C of mushroom producing rooms, to keep mushroom producing room carbon dioxide
Concentration is 0.35%, management of producing mushroom 40 days.Wherein, mycelium management of producing mushroom in mushroom producing room is formed for former base forms phase, mushroom flower bud
Phase, growth period and maturity period.Former base is promoted to form the phase：It is 75%, daily illumination 7h, intensity of illumination to keep cultivation matrix humidity
It is 150 1x, 19 DEG C of maintenance 14h of control temperature, control 12 DEG C of maintenance 10h of temperature daily；Mushroom flower bud forms the phase：Keep cultivation matrix
Humidity is 65%, daily illumination 10h, and intensity of illumination is 600lx, daily 15 DEG C of maintenance 16h of control temperature, 7 DEG C of dimensions of control temperature
Hold 8h；Growth period and maturity period：It is 85%, daily illumination 16h to keep cultivation matrix humidity, and intensity of illumination is 600lx, keeps permanent
22 DEG C of constant temperature degree.
5) harvest：As bacteria cover diameter 3.5-6.5cm, harvesting is prepared during stem 4-6cm long, and 4 days before harvesting, control is planted
Training soil humidity is 65%, and harvesting periodic Control was thoroughly cleaned to mushroom producing room, sterilized in time at 8 days after harvesting.Through plucking
Result data is counted, and is produced per year for every square metre and is reached about 1.8kg.
Embodiment 3
The indoor growing or industrial planting method of a kind of terraced rib hickory chick, comprise the following steps：
1) substrate preparation：Weigh the raw material of following parts by weight：70 parts of peat soil, 10 parts of fertile soil, 5 parts of cow dung, vermiculite 5
Part, 10 parts of liver moss, 10 parts of husk, 8 parts of maize straw, 5 parts of peanut shell, 5 parts of soybean peel；Mixing, be stirring evenly and then adding into containing
The pH value of brown thermophilic actinomycete and bacillus subtilis is 8 potassium phosphate buffer solution sprinkling compound so that compound water
Point content reaches 60%, after being well mixed, stacks 4 days, and daily turning once, that is, obtains cultivation matrix；
2) liquid spawn makes：The parent species of the inclined-plane culture that will have been activated in advance are in an aseptic environment according to parent species amount：Liquid
The percentage by weight of body culture medium is linked into fluid nutrient medium for 3%, is then placed within being cultivated on constant-temperature table, shaking table temperature
22-24 DEG C is controlled to, shaking speed is 120r/min, shaking table culture 5 days.Wherein, fluid nutrient medium is made up of following components：Portugal
Grape sugar 40g/L, yeast extract 30g/L, peptone 30g/L, potassium dihydrogen phosphate 5g/L, magnesium sulfate 5g/L；
3) cultural hypha：Step (2) gained liquid culture bacteria is uniformly sprinkling upon on the cultivation matrix of step (1), then
Start culture after covering 2-3cm cultivation matrixes again, it is 16 DEG C to control culture room temperature, and cultivation matrix humidity is 65%, lucifuge training
Support 25 days；
4) management of producing mushroom：Step (4) mycelium is transferred to temperature in 22 DEG C of mushroom producing rooms, to keep mushroom producing room carbon dioxide
Concentration is 0.3%, management of producing mushroom 45 days.Wherein, mycelium management of producing mushroom in mushroom producing room is formed for former base forms phase, mushroom flower bud
Phase, growth period and maturity period.Former base is promoted to form the phase：It is 80%, daily illumination 8h, intensity of illumination to keep cultivation matrix humidity
It is 100 1x, 22 DEG C of maintenance 16h of control temperature, control 15 DEG C of maintenance 8h of temperature daily；Mushroom flower bud forms the phase：Keep cultivation matrix wet
It is 70% to spend, daily illumination 12h, and intensity of illumination is 200lx, daily 18 DEG C of maintenance 18h of control temperature, the 10 DEG C of maintenances of control temperature
6h；Growth period and maturity period：It is 85%, daily illumination 20h to maintain cultivation matrix humidity, and intensity of illumination is 500lx, is kept constant
22 DEG C of temperature；
5) harvest：As bacteria cover diameter 3.5-6.5cm, harvesting is prepared during stem 4-6cm long, and 5 days before harvesting, control is planted
Training soil humidity is 70%, and harvesting periodic Control was thoroughly cleaned to mushroom producing room, sterilized in time at 10 days after harvesting.Through adopting
Result data statistics is plucked, is produced per year for every square metre and is reached about 1.5kg.
Comparative example
The indoor growing or industrial planting method of a kind of terraced rib hickory chick, comprise the following steps：
(1) preparation of cultivation matrix, (2) liquid spawn make, the condition of (3) cultural hypha it is same as Example 1；
(4) management of producing mushroom：The condition of management of producing mushroom is with reference to documents《Built up one's health after a kind of hickory chick batch production》(CN
105009935 A)：1. mycelium is transferred to the mushroom producing room that temperature is 16 DEG C；2. thermal stimulation：Sclerotium starts shape after 20 days
Into, temperature is reduced to 8 DEG C, cultivation matrix humidity is 50%, and air humidity is 80%, and 6:00 AM is turned on light, and 6 pm turns off the light, light
It is 300lx according to intensity, divulges information as 1000m per hour3Ventilation ventilation 8min；3. thermal stimulation：Temperature is raised after 10 days
It it is 18 DEG C, remaining condition is 1. identical with thermal stimulation, culture is to initially forming fructification；4. fructification culture：Temperature is 18 DEG C,
Air humidity is 80%, is turned on light for 6 points earlier, and 6 pm turns off the light, and cares intensity is 800lx, is divulged information as 1000m per hour3It is logical
Air quantity ventilation 12min, cultivates 20 days, and hickory chick is ripe；(5) Recovery condition is identical with embodiment (1).United through plucking result data
Meter, produces per year for every square metre and reaches about 0.8kg.
Claims (7)
1. a kind of terraced rib hickory chick industrial planting method, it is characterised in that comprise the following steps：
(1) cultivation matrix is prepared：Mainly it is made up of the raw material of following parts by weight：Peat soil 60-70 parts, fertile soil 5-10 parts,
Cow dung 3-5 parts, vermiculite 3-5 parts, liver moss 5-10 parts, husk 5-10 parts, maize straw 5-8 parts, peanut shell 3-5 parts, soybean peel 3-5
Part；
(2) liquid spawn makes：The parent species of the inclined-plane culture that will have been activated are entered in being linked into fluid nutrient medium in an aseptic environment
Row shaking table culture, shaking table culture obtains cultigen after 3-5 days；
(3) cultural hypha：Step (2) gained cultigen is uniformly sprinkling upon on the cultivation matrix obtained by step (1), 2- is then covered by
After cultivation matrix described in 3cm in culturing room lucifuge culture 25-35 days；
(4) management of producing mushroom：Step (3) described mycelium is transferred in mushroom producing room carries out management of producing mushroom, is divided into promotion former base shape
Cheng Qi, mushroom flower bud form phase, growth period and ripe period management：
Former base is promoted to form the phase：Keep the cultivation matrix humidity for 70-80%, daily illumination 6-8h, intensity of illumination is 100-
200 1x, control 10-22 DEG C of temperature；
Mushroom flower bud forms the phase：Keep the cultivation matrix humidity for 60-70%, daily illumination 8-12h, intensity of illumination is 200-
500lx, controls 4-18 DEG C of temperature；
Growth period and maturity period：It is 80-90%, daily illumination 12-20h to keep cultivation matrix humidity, and intensity of illumination is 500-
800lx, keeps 22 DEG C of steady temperature；
Gas concentration lwevel is 0.3-0.4% during management of producing mushroom keeps mushroom producing room, and management of producing mushroom harvests ripe hickory chick for 35-45 days
Fructification.
2. ladder rib hickory chick industrial planting method as claimed in claim 1, it is characterised in that described in blend step (1)
Raw material is constituted, and after stirring, it is 7.5-8.0 phosphorus that sprinkling adds the pH value containing brown thermophilic actinomycete and bacillus subtilis
Acid potassium salt buffer solution so that moisture reaches 50-60% in compound, after being well mixed, stacks 3-4 days, that is, cultivated
Matrix.
3. ladder rib hickory chick industrial planting method as claimed in claim 1, it is characterised in that the liquid described in step (2)
Body culture medium is mainly made up of following components：Glucose 30-40g/L, yeast extract 20-30g/L, peptone 20-30g/L,
Potassium dihydrogen phosphate 3-5g/L, magnesium sulfate 3-5g/L.
4. ladder rib hickory chick industrial planting method as claimed in claim 1, it is characterised in that shaking table culture in step (2)
Condition is：According to the percentage by weight of parent species amount and fluid nutrient medium for be linked into parent species in fluid nutrient medium by 2-3%, then
It is placed on constant-temperature table and cultivates, shaking table temperature is 22-24 DEG C, and shaking speed is 100-120r/min.
5. ladder rib hickory chick industrial planting method as claimed in claim 1, it is characterised in that culture room temperature in step (3)
It is 10-16 DEG C to spend, and the cultivation matrix humidity is 50-65%.
6. ladder rib hickory chick industrial planting method as claimed in claim 1, it is characterised in that in step (4)：
Promotion former base forms the temperature control of phase：16-22 DEG C of maintenance 10-16h of control temperature, controls 10-15 DEG C of temperature daily
Maintain 8-14h；
Mushroom flower bud formed the phase temperature control be：11-18 DEG C of maintenance 14-18h of control temperature, controls 4-10 DEG C of holding 6- of temperature daily
10h。
7. terraced rib hickory chick industrial planting method according to claim 1, it is characterised in that further include harvesting step
Suddenly, as bacteria cover diameter 3.5-6.5cm, harvesting is prepared during stem 4-6cm long, and 3-5 days before harvesting, control cultivation matrix humidity
It is 60-70%, harvesting periodic Control was thoroughly cleaned to mushroom producing room, sterilized in time at 5-10 days after harvesting.
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| CN107311719A (en) * | 2017-06-27 | 2017-11-03 | 安徽诺亚农业有限公司 | A kind of greenhouse gardening method of hickory chick |
| CN107409764A (en) * | 2017-09-29 | 2017-12-01 | 中国科学院昆明植物研究所 | A kind of terraced rib hickory chick autumn planting winter receives cultural method |
| CN107409763A (en) * | 2017-09-29 | 2017-12-01 | 中国科学院昆明植物研究所 | A kind of standardization Production of Large Fields method of six younger sisters hickory chick |
| CN107455145A (en) * | 2017-09-29 | 2017-12-12 | 中国科学院昆明植物研究所 | A kind of cultivation management method of terraced rib hickory chick |
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| CN107409763A (en) * | 2017-09-29 | 2017-12-01 | 中国科学院昆明植物研究所 | A kind of standardization Production of Large Fields method of six younger sisters hickory chick |
| CN107484556A (en) * | 2017-09-29 | 2017-12-19 | 中国科学院昆明植物研究所 | The cultivation of six younger sister hickory chicks and field management method |
| CN107409764A (en) * | 2017-09-29 | 2017-12-01 | 中国科学院昆明植物研究所 | A kind of terraced rib hickory chick autumn planting winter receives cultural method |
| CN107484556B (en) * | 2017-09-29 | 2019-07-19 | 中国科学院昆明植物研究所 | The cultivation of six younger sister hickory chicks and field management method |
| CN107409764B (en) * | 2017-09-29 | 2019-07-19 | 中国科学院昆明植物研究所 | A kind of ladder rib hickory chick autumn planting winter receipts cultural method |
| CN107455145B (en) * | 2017-09-29 | 2019-09-03 | 中国科学院昆明植物研究所 | A kind of cultivation management method of ladder rib hickory chick |
| CN107873389A (en) * | 2017-10-20 | 2018-04-06 | 四川三点水生物科技有限公司 | A kind of production method of hickory chick batch production |
| CN108485995A (en) * | 2018-03-19 | 2018-09-04 | 辽宁省农业科学院 | A kind of complex micro organism fungicide and biological organic fertilizer promoting hickory chick growth |
| CN108782011A (en) * | 2018-07-03 | 2018-11-13 | 山东省科创食用菌产业技术研究院 | A kind of hickory chick breeding method based on Internet of Things |
| CN114946526A (en) * | 2020-12-03 | 2022-08-30 | 成都农业科技职业学院 | Formula and cultivation method of morchella culture material |
| CN114946527A (en) * | 2020-12-03 | 2022-08-30 | 成都农业科技职业学院 | Method for industrially cultivating morchella |
| CN114303791A (en) * | 2022-01-25 | 2022-04-12 | 广西民族师范学院 | Nutrient bag-free industrial cultivation method for morchella esculenta |
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