CN105165389B - A kind of wild ' Ganba ' fungus industrial production method - Google Patents

A kind of wild ' Ganba ' fungus industrial production method Download PDF

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CN105165389B
CN105165389B CN201510418076.0A CN201510418076A CN105165389B CN 105165389 B CN105165389 B CN 105165389B CN 201510418076 A CN201510418076 A CN 201510418076A CN 105165389 B CN105165389 B CN 105165389B
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wizened bacterium
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wizened
soil
wild
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CN105165389A (en
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唐远芝
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Kunming golden Moore edible fungus planting Co., Ltd.
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唐远芝
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms

Abstract

The present invention discloses a kind of wild ' Ganba ' fungus industrial production method, and bacterium strain of drying up is in China Committee for Culture Collection of Microorganisms's common micro-organisms center(CGMCC)Preservation, preserving number is:CGMCC NO. 9902.Bulk is longitudinally torn into after wild ' Ganba ' fungus is sterilized, progress inclined-plane culture in culture medium is inoculated in and obtains wizened bacterium strain.The wizened bacterium strain is inoculated into the culture medium of nutrient bag, the pine roots disinfected are inserted in the nutrient bag simultaneously and cultivated, it is separated into being inoculated in respectively after several parts in above-mentioned identical culture medium and cultivates in the same way, then whole plastic bag cultivation kind is in the soil of pine tree root, with certain condition culture until fructification is exposed after soil 20~30 days, wizened bacterium can be plucked;So realize scale, the industrialized production of wild ' Ganba ' fungus.The wizened mushroom entity of gained of the invention ensure that original fragrance and the nutrition of wizened bacterium.The survival rate of the wizened bacterium of present invention production is high, can reach 99%.

Description

A kind of wild ' Ganba ' fungus industrial production method
Technical field
The invention belongs to wild edible fungus culture technology field, it is related to a kind of wild ' Ganba ' fungus industrial production method.
Background technology
Wizened bacterium(Thelephora ganbajun)Belong to Basidiomycota(Basidiomyeo-ta)Layer bacterium steel (Hymenomyeetes)Aphyllophorales(Aphyhoporales)Lead fungi section(TheLephoraceae)Lead fungi belongs to (Thelephora)In species, be commercially referred to as wizened bacterium also includes orange lead fungi(T.aurantiotineta)It is punished for being related to Lead fungi(T.vialis)Palmate lead fungi(T.palmata)Deng its fructification from the multiple branch of raw coralliform, by the thicker bacterium of base portion Dry tough after softness is old when meat fiber shape keratin is young, gas fragrance is beautiful to be liked by consumers in general deeply, elder brother widely distributed in Yunnan Province The area such as bright, Chuxiong, Yuxi, Lijing, the Baoshan, Qujing, Simao is distributed.
Wizened bacterium is mainly grown in 1000~2200m of height above sea level or so pineland, mainly Yunnan pinon pine in coniferous forest, But also it is distributed in oak-pine mixed forest or pure oakery, it and pine genus plant(Such as pinus yunnanensis and pinus khasys)There is exotrophic mycorrhiza pass System, laterite soil this growing environment that its unique fragrance is had in Pinus Yunnanensis Forest can just be guaranteed, other pine forests Or other soil can not then ensure its unique fragrance.Research finds that the wizened bacteria growing phase is longer, is grown to from mushroom flower bud and is adapted to adopt The fructification plucked needs 20 days or so, and it is aging to grow to fructification, 30~40 days or so is needed, in aerial growth phase, the change of weather Change to bacterium of drying up grow and pest and disease damage generation important, if the air humidity that do not rain for a long time is less than 70%, Sporophore growth is bad, easily occurs wasting disease, and one day light rain rain or shine replaces, and keeps air humidity 80% or so, most has Growth, development beneficial to wizened bacterium, if but it is cloudy and drizzly for days on end for a long time, air humidity is 95% or so, and wizened bacterium easily occurs pink Single-ended spore end disease, therefore wizened bacterium is a kind of very fragile wild-type strain, the best period of harvesting is after fructification is unearthed 20~30 days, the bacterium master recovery time of drying up was June~September, and the wizened bacterium after October is very rare.
Wizened bacterium contains substantial amounts of nutriment, and the content of wherein protein is that 24.15%, crude fibre is 8.45%, total reducing sugar Measure as 3.1%, and the vitamin A in bacterium of drying up per 100g is that 16.0mg, B family vitamin are 1.2mg, and wizened bacterium also contains someone 18 kinds of amino acid necessary to body, linoleic acid content highest therein, also containing new p-terphenyl class pigment, with anti-oxidant work Property, wizened bacterium contains abundant mineral trace element, such as iron, potassium, sodium, phosphorus, calcium selenium.Bacterium of drying up in a word is nutritious, mouth Feel, all the time all in the situation that supply falls short of demand, wild ' Ganba ' fungus sales volume was reduced year by year in recent years, price is soaring tight Weight, is up to 1500 yuan or so, it has also become expensive goods per 1000g prices.Factorial praluction is also not carried out now, therefore Exploitation is a kind of to realize scale, the method for industrialization production wild ' Ganba ' fungus, be to meet the important way needed for people and market Footpath.
The content of the invention
To realize scale, the industrialization production of wizened bacterium, the present invention provides a kind of wild ' Ganba ' fungus factorial praluction side Method.
The present invention is realized by following technical proposal:A kind of wizened bacterium strain, the strain is protected in Chinese microorganism strain Hide administration committee's common micro-organisms center(CGMCC)Preservation, preserving number is:CGMCC NO. 9902, address:Beijing is exposed to the sun The institute 3 of area North Star West Road 1.
Another object of the present invention is to provide a kind of preparation method of wizened bacterium strain, by following each step:
It is that the cleaning of 75% alcohol is dry with volumetric concentration after A, the fresh sturdy wild ' Ganba ' fungus of harvesting, cleaning weeds and soil Bar bacterium surface, aseptically longitudinal direction is torn into bulk afterwards;
B, block wizened bacterium obtained by step A is inoculated in following media:800~1200 parts of maize flour, analysis for soybean powder 800~1200 parts, 300~600 parts of pine tar, 0.2~0.8 part of vitamin B1,0.5~1.5 part of magnesium sulfate, mass concentration be 50% 450~650 parts of glucose solution, mass concentration for 10% 180~300 parts of calcium gluconate solution, potassium dihydrogen phosphate 0.2~ 0.8 part, 40~60 parts of yeast, in carrying out inclined-plane culture at 22~25 DEG C 20~25 days, obtain wizened bacterium strain, its preserving number For:CGMCC NO. 9902
Block wizened bacterium length is 3~5cm, 1~2cm of thickness in the step A.
The inclined-plane culture of the step B is completed in test tube.
A further object of the present invention is to provide a kind of with the wizened bacterium strain industrialized production wild ' Ganba ' fungus Method, comprises the following steps:
(1)It is by preserving number:CGMCC NO. 9902 wizened bacterium strain, is inoculated into the culture medium of nutrient bag, simultaneously In the culture medium that the pine roots disinfected are inserted to the nutrient bag, in being cultivated 25~30 days at 22~25 DEG C, cultivation obtains the Two generations wizened bacterium strain;The culture medium of the nutrient bag is made up of the component of following masses part:80~120 parts of pine, pine needle 20 ~40 parts, 20~40 parts of corn, 20~40 parts of analysis for soybean powder, 5~15 parts of wheat bran, 0.01~0.05 part of gypsum, 5~15 parts of white sugar, 5~15 parts of pine tar, 0.05~0.15 part of yeast;
(2)Take out step(1)The wizened bacterium strain of the gained second generation is divided into after several parts, and the culture medium of nutrient bag is inoculated in respectively In, and insert the pine roots disinfected in every bag of nutrient bag, in being cultivated 25~30 days at 22~25 DEG C, obtain expanding it is numerous after The wizened bacterium strain of the third generation;The same step of culture medium of the nutrient bag(1);
(3)By step(2)Expand the wizened bacterium strain of numerous gained third generation, whole plastic bag cultivation kind is in the soil of pine tree root, plantation Depth be below 10cm, after plantation with sterilization after peat cover, and control temperature for 17~18 DEG C, humidity 70~80%, wind 1~2 grade of power, intensity of illumination are 400~600lux, until fructification is exposed after soil 20~30 days, can pluck wizened bacterium;
(4)With the wizened bacterium of ground under the concordant ground horizontal cutting of knife during harvesting, the wizened bacterium in underground continues to leave, and controls temperature Degree is 17~18 DEG C, humidity 70~80%, 1~2 grade of wind-force, intensity of illumination are 400~600lux, until fructification exposes soil 20~30 days afterwards, and wizened bacterium can be plucked;So realize scale, the industrialized production of wild ' Ganba ' fungus.
The step(1)、(2)In the pine roots disinfected refer to 25~35cm survive, long, 0.5~1cm of diameter Pine tree tree root be cut into inclined-plane, bifurcated is removed, then cleared up and sterilized for 75% alcohol with volumetric concentration, up to without soil Attachment, then inserts 10cm in nutrient bag by tree root, and seals, and keeps dry behaviour's ventilation.
The step(2)Second generation bacterium strain of drying up be divided into several parts and refer to:Second will covered with each bag of nutrient bag In generation, wizened bacterium strain was divided into 15~25 bags.
The step(3)Peat after middle sterilization is to be planted with mass concentration for the corruption after 3~5 ‰ acetic acid solution sprinkling Soil.
The step(3)Pine tree refer to transplant pine tree in greenhouse, and soil comes from pine forests soil, concrete operations It is:Soil movement from pine forests is digged into greenhouse and tiling forms plastic shed soil, between root device will be controlled routinely It is placed in every being vertically placed on plastic shed soil, then by pine tree in control root device, the soil from pine forests is inserted and covered in control root device Lid and fixed pine tree root, and routinely managing and protecting makes pine tree grow more than 1 year.
The pine tree is pinus yunnanensis, and the age of tree is 5~25 years.
The soil is height above sea level in the pine forests that 1500~2200m, pH value are that more than 4.5, organic matter is more than 1.5 Soil.
The height of the control root device is 0.8~1m, a diameter of 0.8~1.2m.The third generation dry up bacterium strain whole plastic bag cultivation kind when Bag number determined depending on the root size of pine tree.
Advantage and effect that the present invention possesses:The present invention can be buried under pine tree root by strain, long-term to preserve old Strain produces spore, utilizes the natural growth rhythm for manually building the Nature;Or wizened bacterium is built into by transplanting breeding The environment that can be grown naturally, adjusts temperature, humidity, luminosity, demeanour all by Automated condtrol, with 5~25 years using warmhouse booth Pinus yunnanensis and Yunnan laterite soil are condition, build the same sandstone soil of mountain forest, then train with breeding strain spore or inclined-plane The mode educated largely obtains wizened bacterium.It will not be made a variation using wizened mushroom entity obtained by the method for the present invention, mouthfeel fragrance and open country Raw wizened bacterium is identical, it is ensured that original fragrance of wizened bacterium and nutrition.The survival rate of the wizened bacterium of present invention production is high, 99% can be reached, and without using agriculture chemical, picking time is short, can pluck once within every 20~30 days, pass through manual control environment Condition, can be achieved scale, the wizened bacterium of industrialization production, and can harvest throughout the year.
Embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1
It is that the cleaning of 75% alcohol is dry with volumetric concentration after A, the fresh sturdy wild ' Ganba ' fungus of harvesting, cleaning weeds and soil Bar bacterium surface, aseptically longitudinal direction is torn into length for 3~5cm, 1~2cm of thickness bulk afterwards;
B, block wizened bacterium obtained by step A is inoculated in following media:1000 parts of maize flour, analysis for soybean powder 1000 Part, 550 parts of the glucose solution that 500 parts of pine tar, 0.5 part of vitamin B1,1 part of magnesium sulfate, mass concentration are 50%, mass concentration For 10% 200 parts of calcium gluconate solution, 0.5 part of potassium dihydrogen phosphate, 50 parts of yeast, in inclined-plane training is carried out at 23 DEG C in test tube Support 22 days, obtain wizened bacterium strain, its preserving number is:CGMCC NO. 9902
C, by preserving number it is:CGMCC NO. 9902 wizened bacterium strain, is inoculated into the culture medium of nutrient bag, will survive , long 30cm, diameter 1cm pine tree tree root be cut into inclined-plane, remove bifurcated, then cleared up for 75% alcohol with volumetric concentration And sterilization, until without soil attachment, tree root then is inserted into 10cm in nutrient bag, and seal, dry behaviour's ventilation is kept, at 25 DEG C Culture 28 days, cultivation obtains the wizened bacterium strain of the second generation;The culture medium of the nutrient bag is made up of the component of following masses part:Pine 100 parts of wood, 30 parts of pine needle, 30 parts of corn, 30 parts of analysis for soybean powder, 10 parts of wheat bran, 0.03 part of gypsum, 10 parts of white sugar, 10 parts of pine tar, ferment It is female 0.1 part;
The wizened bacterium strain of the second generation obtained by D, taking-up step C, by the wizened bacterium bacterium of the second generation covered with each bag of nutrient bag Plant and be divided into after 20 bags, be inoculated in respectively in the culture medium of nutrient bag, 30cm survive, long, diameter 1cm pine tree tree root are cut into Inclined-plane, removes bifurcated, then is cleared up and sterilized for 75% alcohol with volumetric concentration, until without soil attachment, then by tree root Insert 10cm in nutrient bag, and seal, keep dry behaviour's ventilation, in being cultivated 28 days at 25 DEG C, obtain expanding it is numerous after the third generation dry up Bacterium strain;The culture medium of the nutrient bag is with step C;
E, the soil movement from pine forests is digged into greenhouse and tiling forms plastic shed soil, be by height 1m, a diameter of 1m control root device are routinely spaced and are vertically placed on plastic shed soil, then the pine tree that the age of tree is 8 years is placed in into control root In device, the soil from pine forests is inserted into covering and fixed pine tree root in control root device, and routinely managing and protecting grows pine tree More than 1 year;Step D is expanded into the numerous gained third generation to dry up bacterium strain, whole plastic bag cultivation kind in control root device in the soil of pine tree root, Planting depth is 15cm, plant 2 bags, after plantation with sterilization after peat cover, and control temperature for 18 DEG C, humidity 80%, wind 1~2 grade of power, intensity of illumination are 500lux, until fructification is exposed after soil 20~30 days, can pluck wizened bacterium;It is described to disappear Peat after poison is the peat after being sprayed with mass concentration for 4 ‰ acetic acid solution;
With the wizened bacterium of ground under the concordant ground horizontal cutting of knife when F, harvesting, the wizened bacterium in underground continues to leave, and controls temperature Degree is 18 DEG C, humidity 80%, 1~2 grade of wind-force, intensity of illumination are 500lux, until fructification exposes after soil 20~30 days, again Can the wizened bacterium of harvesting;So realize scale, the industrialized production of wild ' Ganba ' fungus.Survival rate 99%.
Embodiment 2
It is that the cleaning of 75% alcohol is dry with volumetric concentration after A, the fresh sturdy wild ' Ganba ' fungus of harvesting, cleaning weeds and soil Bar bacterium surface, aseptically longitudinal direction is torn into length for 3cm, thickness 1cm bulk afterwards;
B, block wizened bacterium obtained by step A is inoculated in following media:800 parts of maize flour, 800 parts of analysis for soybean powder, 450 parts of glucose solution, mass concentration that 600 parts of pine tar, 0.2 part of vitamin B1,1.5 parts of magnesium sulfate, mass concentration are 50% For 10% 300 parts of calcium gluconate solution, 0.2 part of potassium dihydrogen phosphate, 40 parts of yeast, in inclined-plane training is carried out at 22 DEG C in test tube Support 25 days, obtain wizened bacterium strain, its preserving number is:CGMCC NO. 9902
C, by preserving number it is:CGMCC NO. 9902 wizened bacterium strain, is inoculated into the culture medium of nutrient bag, will survive , long 25cm, diameter 0.8cm pine tree tree root be cut into inclined-plane, remove bifurcated, then carried out clearly with the alcohol that volumetric concentration is 75% Reason and sterilization, until without soil attachment, tree root then is inserted into 10cm in nutrient bag, and are sealed, and keep doing behaviour's ventilation, in 23 DEG C Lower culture 30 days, cultivation obtains the wizened bacterium strain of the second generation;The culture medium of the nutrient bag is made up of the component of following masses part: 80 parts of pine, 20 parts of pine needle, 40 parts of corn, 20 parts of analysis for soybean powder, 5 parts of wheat bran, 0.05 part of gypsum, 5 parts of white sugar, 15 parts of pine tar, ferment It is female 0.05 part;
The wizened bacterium strain of the second generation obtained by D, taking-up step C, by the wizened bacterium bacterium of the second generation covered with each bag of nutrient bag Plant and be divided into after 15 bags, be inoculated in respectively in the culture medium of nutrient bag, 25cm survive, long, diameter 0.8cm pine tree tree root are cut Into inclined-plane, bifurcated is removed, then is cleared up and is sterilized for 75% alcohol with volumetric concentration, until without soil attachment, then will tree 10cm in root insertion nutrient bag, and is sealed, and keeps dry behaviour's ventilation, in being cultivated 30 days at 22 DEG C, obtain expanding it is numerous after the third generation do Bar bacterium strain;The culture medium of the nutrient bag is with step C;
E, by height above sea level in 1500~2200m, pH value it is the soil from pine forests that more than 4.5, organic matter is more than 1.5 Movement is digged into greenhouse and tiling forms plastic shed soil, will height for 0.8m, a diameter of 1.2m control root device routinely Interval is vertically placed on plastic shed soil, then the age of tree was placed in control root device for the pinus yunnanensis of 5 years, by the soil from pine forests Covering and fixed pine tree root in control root device are inserted, and routinely managing and protecting makes pine tree grow more than 1 year;Step D is expanded into numerous gained The wizened bacterium strain of the third generation, whole plastic bag cultivation kind is in control root device in the soil of pine tree root, and planting depth is 12cm, plants 1 bag, plants Covered after kind with the peat after sterilization, and to control temperature be 17 DEG C, humidity 70%, 1 grade of wind-force, intensity of illumination are 400lux, directly Expose to fructification after soil 20 days, wizened bacterium can be plucked;Peat after the sterilization is with the second that mass concentration is 3 ‰ Peat after acid solution sprinkling;
With the wizened bacterium of ground under the concordant ground horizontal cutting of knife when F, harvesting, the wizened bacterium in underground continues to leave, and controls temperature Degree is 17 DEG C, humidity 70%, 1 grade of wind-force, intensity of illumination are 400lux, until fructification is exposed after soil 20 days, and can pluck and do Bar bacterium;So realize scale, the industrialized production of wild ' Ganba ' fungus.Survival rate 98.9%.
Embodiment 3
It is that the cleaning of 75% alcohol is dry with volumetric concentration after A, the fresh sturdy wild ' Ganba ' fungus of harvesting, cleaning weeds and soil Bar bacterium surface, aseptically longitudinal direction is torn into length for 5cm, thickness 2cm bulk afterwards;
B, block wizened bacterium obtained by step A is inoculated in following media:1200 parts of maize flour, analysis for soybean powder 1200 Part, 650 parts of the glucose solution that 300 parts of pine tar, 0.8 part of vitamin B1,0.5 part of magnesium sulfate, mass concentration are 50%, quality are dense 180 parts of the calcium gluconate solution, 0.8 part of potassium dihydrogen phosphate, 60 parts of yeast for 10% are spent, in carrying out inclined-plane at 25 DEG C in test tube Culture 20 days, obtains wizened bacterium strain, and its preserving number is:CGMCC NO. 9902
C, by preserving number it is:CGMCC NO. 9902 wizened bacterium strain, is inoculated into the culture medium of nutrient bag, will survive , long 35cm, diameter 0.5cm pine tree tree root be cut into inclined-plane, remove bifurcated, then carried out clearly with the alcohol that volumetric concentration is 75% Reason and sterilization, until without soil attachment, tree root then is inserted into 10cm in nutrient bag, and are sealed, and keep doing behaviour's ventilation, in 22 DEG C Lower culture 25 days, cultivation obtains the wizened bacterium strain of the second generation;The culture medium of the nutrient bag is made up of the component of following masses part: 120 parts of pine, 40 parts of pine needle, 20 parts of corn, 40 parts of analysis for soybean powder, 15 parts of wheat bran, 0.01 part of gypsum, 15 parts of white sugar, 5 parts of pine tar, 0.15 part of yeast;
The wizened bacterium strain of the second generation obtained by D, taking-up step C, by the wizened bacterium bacterium of the second generation covered with each bag of nutrient bag Plant and be divided into after 25 bags, be inoculated in respectively in the culture medium of nutrient bag, 35cm survive, long, diameter 0.5cm pine tree tree root are cut Into inclined-plane, bifurcated is removed, then is cleared up and is sterilized for 75% alcohol with volumetric concentration, until without soil attachment, then will tree 10cm in root insertion nutrient bag, and is sealed, and keeps dry behaviour's ventilation, in being cultivated 25 days at 23 DEG C, obtain expanding it is numerous after the third generation do Bar bacterium strain;The culture medium of the nutrient bag is with step C;
E, by height above sea level 1500~2200m, pH value be 6, organic matter be 2 the soil movement from pine forests into greenhouse Digged and tiling form plastic shed soil, will height be 1m, a diameter of 0.8m control root device is routinely spaced and is vertically placed on On plastic shed soil, then the age of tree is placed in control root device for the pinus yunnanensis of 15~25 years, the soil from pine forests is inserted into control root Covered in device and fixed pine tree root, and routinely managing and protecting makes pine tree grow more than 1 year;Step D is expanded into numerous gained third generation to do Bar bacterium strain, whole plastic bag cultivation kind is in control root device in the soil of pine tree root, and planting depth is below 10cm, plants 3 bags, after plantation Covered with the peat after sterilization, and to control temperature be 17~18 DEG C, humidity 75%, 2 grades of wind-force, intensity of illumination are 600lux, directly Expose to fructification after soil 30 days, wizened bacterium can be plucked;Peat after the sterilization is with the second that mass concentration is 5 ‰ Peat after acid solution sprinkling;
With the wizened bacterium of ground under the concordant ground horizontal cutting of knife when F, harvesting, the wizened bacterium in underground continues to leave, and controls temperature Degree is 17~18 DEG C, humidity 75%, 2 grades of wind-force, intensity of illumination are 600lux, until fructification is exposed after soil 30 days, can be adopted again Pluck wizened bacterium;So realize scale, the industrialized production of wild ' Ganba ' fungus.Survival rate 99.2%.

Claims (3)

1. a kind of wizened bacterium strain, it is characterised in that:The strain is commonly micro- in China Committee for Culture Collection of Microorganisms Bio-Centers(CGMCC)Preservation, preserving number is:CGMCC NO. 9902;
The wizened bacterium strain is prepared through the following steps:
It is the wizened bacterium of 75% alcohol cleaning with volumetric concentration after A, the fresh sturdy wild ' Ganba ' fungus of harvesting, cleaning weeds and soil Surface, aseptically longitudinal direction is torn into bulk afterwards;
B, block wizened bacterium obtained by step A is inoculated in following media:800~1200 parts of maize flour, analysis for soybean powder 800~ 1200 parts, 300~600 parts of pine tar, 0.2~0.8 part of vitamin B1,0.5~1.5 part of magnesium sulfate, mass concentration be 50% Portugal 180~300 parts of calcium gluconate solution, the potassium dihydrogen phosphate 0.2~0.8 of 450~650 parts of grape sugar juice, mass concentration for 10% Part, 40~60 parts of yeast, in carrying out inclined-plane culture at 22~25 DEG C 20~25 days, obtain wizened bacterium strain, its preserving number is: CGMCC NO. 9902。
2. wizened bacterium strain according to claim 1, it is characterised in that:Block wizened bacterium length is 3 in the step A ~5cm, 1~2cm of thickness.
3. wizened bacterium strain according to claim 1, it is characterised in that:The inclined-plane culture of the step B is in test tube Complete.
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CN105532265A (en) * 2016-02-02 2016-05-04 李永华 Wild sparassia crispa cultivation and original ecology planting method
CN106212042A (en) * 2016-07-04 2016-12-14 李业武 A kind of pseudo-wild cultivating method of Tricholoma matsutake (lto et lmai) Singer
CN106676015A (en) * 2016-12-15 2017-05-17 防城港市蓝瀚达科技有限公司 Preparation method of dried strain potato culture medium
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CN110463508B (en) * 2019-09-09 2021-04-23 陕西科技大学 Method for artificially forming Thelephora ganbajun pond

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