CN105420114A - Dictyophora rubrovalvata solid mother strain cultivation method - Google Patents
Dictyophora rubrovalvata solid mother strain cultivation method Download PDFInfo
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- CN105420114A CN105420114A CN201510841397.1A CN201510841397A CN105420114A CN 105420114 A CN105420114 A CN 105420114A CN 201510841397 A CN201510841397 A CN 201510841397A CN 105420114 A CN105420114 A CN 105420114A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a dictyophora rubrovalvata solid mother strain cultivation method. The dictyophora rubrovalvata solid mother strain cultivation method includes the following steps that cultivation materials including 73-77 parts of sawdust, 16-20 parts of wheat bran, 1-5 parts of corn flour, 1-3 parts of soybean flour, 1-1.5 parts of white sugar and 1-1.5 parts of gypsum are weighed by weight; the various cultivation materials are mixed evenly, water accounting for 58-62% of the total mass of the cultivation materials is added, the mixture is filled into a conventional strain bottle after even stirring, and the strain bottle filled with the cultivation materials is steamed and cooked in a waterproof mode and then is subjected to standing for standby application; mature bamboo fungi are flushed with alcohol, and the flushed mature bamboo fungi are put into a conventional inoculating box to separate tissue blocks; the tissue blocks are inoculated into a standby strain bottle; the strain bottle is arranged in a room with the temperature of 22-25 DEG C and the humidity of 55-65% to perform cultivation for 35-45 days after inoculation is completed, and a dictyophora rubrovalvata solid mother strain is obtained. The dictyophora rubrovalvata solid mother strain cultivation method has the advantages of being short in period, high in yield, good in quality and low in cost.
Description
Technical field
The invention belongs to field of plant growing technology, be specifically related to the cultural method that a kind of Dictyophora rubrovalvata solid mother plants.
Background technology
Tradition dictyophora phalloidea mother plants main based on test tube kind, takes separate tissue mode to cultivate and obtains.Its step to be seeded on test tube nutrient agar for selecting ripe bamboo egg-chorista block-nutrient agar-by the dictyophora phalloidea tissue block of separator well-and greenhouse sterile culture to test tube mycelia cover with-is seeded on Mother culture material (bottle) and cultivates.
Produce Dictyophora rubrovalvata mother in this way and plant the problem that there are following 3 aspects:
(1) need the time of about 5 wheat harvesting periods, wherein, test tube kind mycelia cover with need 40 days, be seeded to bottled kind and need 145-150 days;
(2) need to carry out twice inoculation, add the probability that bacterial classification is infected;
(3) one pipe test tubes can meet at most 2-3 bottle mother and plant.
The problems referred to above directly cause dictyophora phalloidea mother kind Productive statistics high, and the cycle is long and benefit is low, can not meet the demand in dictyophora phalloidea bacterial classification market far away, greatly constrain the development of dictyophora phalloidea industry.
China Patent Publication No. is the application for a patent for invention of CN104920065A, discloses " the wild Dictyophora rubrovalvata mother of a kind of Mount Fanjing plants making method ", but there is dramatic difference with the technology of the present invention method.
Summary of the invention
The object of the present invention is to provide that a kind of cycle is short, output is high, cultural method that quality better and lower-cost Dictyophora rubrovalvata solid mother plant.
The cultural method that a kind of Dictyophora rubrovalvata solid mother of the present invention plants, comprises the following steps:
(1) following culture material is taken by weight: wood chip 73-77 part, wheat bran 16-20 part, Semen Maydis powder 1-5 part, analysis for soybean powder 1-3 part, white sugar 1-1.5 part, gypsum 1-1.5 part; Each culture material is mixed, adds the water of each culture material total mass 58-62%, load in conventional seed bottle after stirring; The seed bottle water proof boiling 20-24h of culture material will be housed, and take out and leave standstill 20-30h, for subsequent use;
(2) get ripe bamboo egg with alcohol rinse, after flushing, ripe bamboo egg is put into traditional vaccination case and carry out tissue block separation, ripe bamboo egg is separated into the tissue block that 0.25-0.3cm is long, 0.25-0.3cm is wide;
(3) tissue block accessed in seed bottle for subsequent use, every bottle graft enters tissue block 5-7 grain, and access position is each one of seed bottle top, middle part and bottom, and all the other are connected on seed bottle bottle wall;
(4), after having inoculated, seed bottle is placed in the indoor cultivation 35-45 days that temperature is 22-25 DEG C, humidity is 55-65%, obtains final product.
The cultural method that above-mentioned a kind of Dictyophora rubrovalvata solid mother plants, wherein: the culture material taken by weight in step (1) is: wood chip 75 parts, 18 parts, wheat bran, Semen Maydis powder 3 parts, analysis for soybean powder 2 parts, white sugar 1 part, 1 part, gypsum.
The present invention compared with prior art, there is obvious beneficial effect, as can be known from the above technical solutions: the cultural method that Dictyophora rubrovalvata solid mother provided by the present invention plants, distinguish traditional production of hybrid seeds pattern, selected homemade culture material formula, tissue block granular size and multiple spot inoculation method.Described culture material formula is balanced in nutrition, and natural nothing is added, and is conducive to dictyophora phalloidea mycelial growth, fruiting body differentiation, and then improves dictyophora phalloidea output and dictyophora phalloidea quality; The restriction of tissue block granular size and the applicable of multiple spot inoculation method make mycelia become multiple spot sprouting and multiple spot material feeding, substantially increase the speed of growth of mycelia; Each technological step and processing parameter are worked in coordination with mutually, by traditional secondary inoculation successful conversion for once to inoculate, reduce the probability of mycelia superinfection, mother is planted the production cycle and brought up to 1-1.5 month by the 4.5-5 in traditional method month, create fabulous economic benefit and social benefit.Have that the cycle is short, output is high, quality better and lower-cost feature.
Embodiment
embodiment 1
The cultural method that Dictyophora rubrovalvata solid mother plants, comprises the following steps:
(1) following culture material is taken by weight: wood chip 73g, wheat bran 16g, Semen Maydis powder 1g, analysis for soybean powder 3g, white sugar 1.5g, gypsum 1.5g; Each culture material is mixed, adds the water of each culture material total mass 58%, load in conventional seed bottle after stirring; The seed bottle water proof boiling 20h of culture material will be housed, and take out and leave standstill 20h, for subsequent use;
(2) get ripe bamboo egg with alcohol rinse, after flushing, ripe bamboo egg is put into traditional vaccination case and carry out tissue block separation, ripe bamboo egg is separated into the tissue block that 0.25cm is long, 0.3cm is wide;
(3) tissue block accessed in seed bottle for subsequent use, every bottle graft enters tissue block 7, and access position is each one of seed bottle top, middle part and bottom, and all the other are connected on seed bottle bottle wall;
(4), after having inoculated, seed bottle is placed in temperature is 22 DEG C, humidity be 55% indoor cultivation 35 days, to obtain final product.
embodiment 2
The cultural method that Dictyophora rubrovalvata solid mother plants, comprises the following steps:
(1) following culture material is taken by weight: wood chip 77g, wheat bran 20g, Semen Maydis powder 5g, analysis for soybean powder 1g, white sugar 1g, gypsum 1g; Each culture material is mixed, adds the water of each culture material total mass 60%, load in conventional seed bottle after stirring; The seed bottle water proof boiling 24h of culture material will be housed, and take out and leave standstill 30h, for subsequent use;
(2) get ripe bamboo egg with alcohol rinse, after flushing, ripe bamboo egg is put into traditional vaccination case and carry out tissue block separation, ripe bamboo egg is separated into the tissue block that 0.3cm is long, 0.25cm is wide;
(3) tissue block accessed in seed bottle for subsequent use, every bottle graft enters tissue block 5, and access position is each one of seed bottle top, middle part and bottom, and all the other are connected on seed bottle bottle wall;
(4), after having inoculated, seed bottle is placed in temperature is 25 DEG C, humidity be 65% indoor cultivation 45 days, to obtain final product.
embodiment 3
The cultural method that Dictyophora rubrovalvata solid mother plants, comprises the following steps:
(1) following culture material is taken by weight: wood chip 75g, wheat bran 18g, Semen Maydis powder 3g, analysis for soybean powder 2g, white sugar 1.25g, gypsum 1.25g; Each culture material is mixed, adds the water of each culture material total mass 59%, load after stirring in conventional seed bottle, get cotton and bottleneck is clogged, then cover bottleneck with kraft paper and fix with bungee; The seed bottle water proof boiling 22h of culture material will be housed, and take out and leave standstill 25h, for subsequent use;
(2) get ripe bamboo egg with alcohol rinse, after flushing, ripe bamboo egg is put into traditional vaccination case and carry out tissue block separation, ripe bamboo egg is separated into the tissue block that 0.27cm is long, 0.27cm is wide;
(3) tissue block is accessed in seed bottle for subsequent use, get cotton equally to be clogged by bottleneck, then cover bottleneck with kraft paper and fix with bungee, every bottle graft enters tissue block 6, access position is each one of seed bottle top, middle part and bottom, and all the other are connected on seed bottle bottle wall;
(4), after having inoculated, seed bottle is placed in temperature is 23.5 DEG C, humidity be 57.5% indoor cultivation 37 days, to obtain final product.
embodiment 4
The cultural method that Dictyophora rubrovalvata solid mother plants, comprises the following steps:
(1) following culture material is taken by weight: wood chip 75g, wheat bran 18g, Semen Maydis powder 3g, analysis for soybean powder 2g, white sugar 1g, gypsum 1g; Each culture material is mixed, adds the water of each culture material total mass 59%, load after stirring in conventional seed bottle, get cotton and bottleneck is clogged, then cover bottleneck with kraft paper and fix with bungee; The seed bottle water proof boiling 21h of culture material will be housed, and take out and leave standstill 26h, for subsequent use;
(2) get ripe bamboo egg with alcohol rinse, after flushing, ripe bamboo egg is put into traditional vaccination case and carry out tissue block separation, ripe bamboo egg is separated into the tissue block that 0.28cm is long, 0.28cm is wide;
(3) tissue block is accessed in seed bottle for subsequent use, get cotton equally to be clogged by bottleneck, then cover bottleneck with kraft paper and fix with bungee, every bottle graft enters tissue block 6, access position is each one of seed bottle top, middle part and bottom, and all the other are connected on seed bottle bottle wall;
(4), after having inoculated, seed bottle is placed in temperature is 24 DEG C, humidity be 58% indoor cultivation 38 days, to obtain final product.
The above, it is only preferred embodiment of the present invention, not any pro forma restriction is done to the present invention, anyly do not depart from technical solution of the present invention content, the any simple modification done above embodiment according to technical spirit of the present invention, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (2)
1. a cultural method for Dictyophora rubrovalvata solid mother kind, comprises the following steps:
(1) following culture material is taken by weight: wood chip 73-77 part, wheat bran 16-20 part, Semen Maydis powder 1-5 part, analysis for soybean powder 1-3 part, white sugar 1-1.5 part, gypsum 1-1.5 part; Each culture material is mixed, adds the water of each culture material total mass 58-62%, load in conventional seed bottle after stirring; The seed bottle water proof boiling 20-24h of culture material will be housed, and take out and leave standstill 20-30h, for subsequent use;
(2) get ripe bamboo egg with alcohol rinse, after flushing, ripe bamboo egg is put into traditional vaccination case and carry out tissue block separation, ripe bamboo egg is separated into the tissue block that 0.25-0.3cm is long, 0.25-0.3cm is wide;
(3) tissue block accessed in seed bottle for subsequent use, every bottle graft enters tissue block 5-7 grain, and access position is each one of seed bottle top, middle part and bottom, and all the other are connected on seed bottle bottle wall;
(4), after having inoculated, seed bottle is placed in the indoor cultivation 35-45 days that temperature is 22-25 DEG C, humidity is 55-65%, obtains final product.
2. the cultural method of a kind of Dictyophora rubrovalvata solid mother kind as claimed in claim 1, wherein: the culture material taken by weight in step (1) is: wood chip 75 parts, 18 parts, wheat bran, Semen Maydis powder 3 parts, analysis for soybean powder 2 parts, white sugar 1 part, 1 part, gypsum.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510841397.1A CN105420114A (en) | 2015-11-28 | 2015-11-28 | Dictyophora rubrovalvata solid mother strain cultivation method |
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| CN201510841397.1A CN105420114A (en) | 2015-11-28 | 2015-11-28 | Dictyophora rubrovalvata solid mother strain cultivation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754394A (en) * | 2016-07-10 | 2017-05-31 | 贵州省农作物品种资源研究所 | It is suitable for the Dictyophora rubrovalvata yzs020 bacterial strains of substituting stuff cultivation |
| CN110100657A (en) * | 2019-06-03 | 2019-08-09 | 贵州织金毫米农业科技发展有限公司 | A kind of production method of Dictyophora rubrovalvata test tube stock |
| CN110367047A (en) * | 2019-06-03 | 2019-10-25 | 贵州织金毫米农业科技发展有限公司 | A kind of Dictyophora rubrovalvata test tube stock culture medium and its application |
| CN111394252A (en) * | 2020-03-27 | 2020-07-10 | 江苏华绿生物科技股份有限公司 | Preservation strain activation transfer method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103650922A (en) * | 2013-12-27 | 2014-03-26 | 贵州省农作物品种资源研究所 | Method for cultivating dictyophora rubrovolvata from mushroom sticks in bag-removing and soil-covering mode through maize straw fermented materials and decomposed materials |
-
2015
- 2015-11-28 CN CN201510841397.1A patent/CN105420114A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103650922A (en) * | 2013-12-27 | 2014-03-26 | 贵州省农作物品种资源研究所 | Method for cultivating dictyophora rubrovolvata from mushroom sticks in bag-removing and soil-covering mode through maize straw fermented materials and decomposed materials |
Non-Patent Citations (2)
| Title |
|---|
| 刘芳 等: "红托竹荪母种培养基配方的筛选试验", 《乐山师范学院学报》 * |
| 吴勇: "织金竹荪驯化栽培研究", 《贵州师范大学学报（自然科学版）》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754394A (en) * | 2016-07-10 | 2017-05-31 | 贵州省农作物品种资源研究所 | It is suitable for the Dictyophora rubrovalvata yzs020 bacterial strains of substituting stuff cultivation |
| CN106754394B (en) * | 2016-07-10 | 2021-11-12 | 贵州省农作物品种资源研究所 | Dictyophora rubrovalvata yzs020 strain suitable for substitute cultivation |
| CN110100657A (en) * | 2019-06-03 | 2019-08-09 | 贵州织金毫米农业科技发展有限公司 | A kind of production method of Dictyophora rubrovalvata test tube stock |
| CN110367047A (en) * | 2019-06-03 | 2019-10-25 | 贵州织金毫米农业科技发展有限公司 | A kind of Dictyophora rubrovalvata test tube stock culture medium and its application |
| CN111394252A (en) * | 2020-03-27 | 2020-07-10 | 江苏华绿生物科技股份有限公司 | Preservation strain activation transfer method |
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