CN104591908A - Canoderma lucidum culture medium and preparation method thereof - Google Patents

Canoderma lucidum culture medium and preparation method thereof Download PDF

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Publication number
CN104591908A
CN104591908A CN201510064750.XA CN201510064750A CN104591908A CN 104591908 A CN104591908 A CN 104591908A CN 201510064750 A CN201510064750 A CN 201510064750A CN 104591908 A CN104591908 A CN 104591908A
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matrix
cultivation
ganoderma
glossy ganoderma
major ingredient
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CN201510064750.XA
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CN104591908B (en
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张进
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Kang Tao biological (Nantong) Co., Ltd.
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张进
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00Calcareous fertilisers
    • C05D3/02Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention relates to an edible mushroom culture medium and in particular relates to a canoderma lucidum culture medium and a preparation method thereof. The canoderma lucidum culture medium is prepared by mixing a main material with auxiliary materials, wherein the main material accounts for 25-60% of total weight of the medium, and is one or a combination of several of mulberry branch, soybean hull and paper mulberry saw dust; the auxiliary materials include the following components in percentage by mass: 10-15% of rape seed cake, 0.1-10% of canoderma lucidum bacterium powder, 1-3% of calcium carbonate, 1-3% of fermented silkworm manure, 0.05-0.1% of sodium humate and the balance of lycium chinense mill powder; the canoderma lucidum bacterium powder is powder containing canoderma lucidum sporophore. The culture medium is reasonable in nutrient substances, and satisfies phosphorus and other vitamin substances needed by the growth of the canoderma lucidum; the raw materials are widely available and low in cost; the cultured canoderma lucidum sporophore is similar to the natural wild canoderma lucidum in nutritional ingredients.

Description

A kind of cultivation of glossy ganoderma matrix and preparation method thereof
Technical field
The present invention relates to a kind of edible fungus culturing matrix, especially relate to a kind of cultivation of glossy ganoderma matrix and preparation method thereof.
Background technology
Glossy ganoderma (formal name used at school: Ganoderma Lucidum Karst), profile is umbrella, cap kidney shape, semicircle or subcircular, is the sporophore of On Polyporaceae glossy ganoderma.There is invigorating QI and tranquilization, relieving cough and asthma effect, cough breathe heavily for dizzy egersis, shortness of breath and palpitation, consumptive disease.
Ganoderma sporophore is the mushroom shape thing of a umbrella shape, in red-purple or red-brown, is meat during its quality children, ripe become dry after be suberin, sporophore is made up of cap and stem.Present clinical study shows, Ganoderma sporophore there is abundant pharmacological action:
1, to the restraining effect of tumour.The ganoderan extracted from Ganoderma sporophore is considered to glossy ganoderma to be had antitumor and has the principle active component of immune physiologically active, and transplanting S180 sarcoma as Polysaccharides on Mice has restraining effect, inhibiting rate 54.1%-87.6%.
2, glossy ganoderma can anti-radiotherapy damage.Polysaccharide D6 in ganoderan can promote medullary cell protein and nucleic acid synthesis, and accelerated bone myelocyte division growth, stimulates the hematopoietic function of marrow, makes the mouse by Co 60 radiation exposure extend the survival time.
3, glossy ganoderma has calmness, stable and analgesic activity to neural system.
4, glossy ganoderma has cardiac stimulant, Ischemic myocardium, step-down and reducing blood lipid to cardiovascular.
5, glossy ganoderma has antibechic, antiasthmatic effect, has therapeutic action to chronic tracheitis, draws the injected in mice glossy ganoderma Aqueous extracts or mycelia extract coughed or glossy ganoderma fermentation concentrated solution has obvious antitussive effect with ammoniacal liquor, and can make to draw to cough prolongation of latency, cough number of times reduces.
6, glossy ganoderma has preventive and therapeutic effect to hepatitis.
7, glossy ganoderma has hypoglycemic and oxygen lack resistant function.Ganoderma A, B, C in ganoderan can accelerate pancreas islet blood circulation, improve islet cells physiological function and excreting insulin ability, reduce hepatic glycogen content.Glossy ganoderma total alkali can improve hypoxia-bearing capability 30%.
Current glossy ganoderma artificial culture has cultivation basswood and substituting stuff cultivation, and cultivation basswood is the method adopting the preferential species of tree section of being cut into cultivating ganoderma, and the glossy ganoderma plant type that the method obtains is good, and quality is good, but the method needs to take a large amount of land resources.Substituting stuff cultivation is a kind of with wood chip, and cotton seed hulls, bagasse, agricultural crop straw, waits the method for agricultural tankage cultivating ganoderma.Although existing various Medium for Ganoderma lucidum matter can obtain relatively satisfied output, cultivation period is longer, and autumn culture needs the winter crossing over low temperature, increases handling cost.But it is higher that conventional cultivation of glossy ganoderma matrix still has cost, and planting time is relatively long, nutritional imbalance, the technical barrier that the utilization ratio of nutrient is low, the glossy ganoderma product of output are still undesirable mutually.
Summary of the invention
The present invention in order to the cultivation of glossy ganoderma matrix cost solving prior art higher, the utilization ratio of nutrient is low, the defect that cultivation period is long, and provide a kind of scientific formulation reasonable, cost is low, nutritive equilibrium, can improve the cultivation of glossy ganoderma matrix of glossy ganoderma output.
The invention also discloses a kind of preparation method of cultivation of glossy ganoderma matrix, this preparation method's processing step is simple, and workable, each component mixing uniformity is good, is applicable to suitability for industrialized production.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of cultivation of glossy ganoderma matrix, described cultivation of glossy ganoderma matrix is mixed by major ingredient and auxiliary material, major ingredient accounts for the 25-60% of matrix gross weight, major ingredient is the combination of one or more in mulberry branch, soybean peel, paper mulberry wood chip, and auxiliary material comprises following in the component of massfraction: rapeseed cake 10-15%, ganoderma lucidum powder 0.1-10%, calcium carbonate 1-3%, fermentation silkworm faeces 1 ~ 3%, Sodium salts humic acids 0.05 ~ 0.1%, surplus is great Ye Chinese holly grass meal; Described ganoderma lucidum powder is the powder containing Ganoderma sporophore.
The present invention according to glossy ganoderma at the Nutritional selectivity of Different growth phases to cultivation matrix, grow the nutrient demand in cultivation matrix according to glossy ganoderma again, optimize cultivation matrix, the present invention abandons traditional, content and the high wheat bran of cost as organic nitrogen source, replaces with the mixing organic nitrogen source of major ingredient, rapeseed cake, great Ye Chinese holly grass meal and fermentation silkworm faeces composition.Adopt mulberry branch, soybean peel, paper mulberry wood chip to instead of the wood chip of traditional large usage quantity, be to reduce costs on the one hand, on the other hand, the ventilation property of matrix can be increased.Adopt the great Ye Chinese holly grass meal that Relative Size is larger, between matrix, gap is larger, and good permeability, and the usage quantity that can reduce wheat bran, be conducive to reducing costs.In order to shorten incubation time, in substratum of the present invention, also adding appropriate ganoderma lucidum powder, proving that this cultivation medium formula can shorten the time of Ganoderma mycelium amount reproduction growth through culture experiment.
Cultivation medium formula of the present invention is scientific and reasonable, and cost is low, nutritive equilibrium, can improve glossy ganoderma output.Experiment in cultivation proves, the combination of mulberry branch, soybean peel, paper mulberry wood chip can significantly improve glossy ganoderma total reducing sugar and protein content, and similar with the effect of wood chip, straw, cotton seed hulls in all the other indexs.
Great Ye Chinese holly belongs to broadleaf woody plants, be in recent years through breed breeding, introduce a fine variety novel forage grass on local hill covered with grass grass slope and ground without fixed duties, output is high, and in its dry matter weight of leaf, crude protein content reaches more than 20%.Studies have found that, great Ye Chinese holly is compared with dregs of beans with alfalfa meal, crude protein content: dregs of beans > great Ye Chinese holly leaf > alfalfa meal, neutral detergent fiber, acid detergent fiber content are then contrary, the Mineral Concentrations such as iron, manganese, zinc, cobalt, iodine, great Ye Chinese holly is higher than alfalfa meal and dregs of beans, and most aminoacids content comparatively alfalfa meal is high, is a kind of green, efficient feed resource.Current great Ye Chinese holly is used for field of fodder, the present invention is through studying for a long period of time, find the great Ye Chinese holly adding appropriate ratio in the substratum of mushroom, be conducive to the quick growth of bacterium massee fruiting bodies, relative shortens its cultivation period, meanwhile, cultivate the bacterium massee fruiting bodies Mineral Element Contents obtained higher, in addition containing a small amount of Flavonoid substances.This may be because great Ye Chinese holly comprehensive nutrition is balanced, is rich in pure natural flavonoid physiologically active substance, absorbs the various nutritive substances in matrix during mycelial growth in stem stalk and blade.
Rapeseed cake (dregs of rice) is the byproduct thing after rapeseed oil, and nutritive value is higher; Containing higher protein, amino acid composition comparatively balances, and sulfur amino acid content is high, and arginine content is lower, and lysine content is low.Crude fiber content is higher, and calcic is also higher.Phosphorus is higher than calcium, and major part is phytate phosphorus.Trace element iron content is abundanter.The content of its crude protein is 31.5%, digestible protein 25.6%, also containing the trace element such as amino acid and manganese, zinc, copper.Phosphorus is a kind of main composition composition of glossy ganoderma cell core, and other mineral elements are play a part to improve enzymic activity as coenzyme material mostly, and requirement is very micro-, so the phosphorus that glossy ganoderma growth needs is a large amount of.Contriver finds in long-term mushroom is cultivated, in the matrix of glossy ganoderma growth, add appropriate rapeseed cake, and can grow for glossy ganoderma the phosphorus providing enough content, the glossy ganoderma surface gloss of production is fine---glossy shinny, and mushroom body consolidation, sporophore is larger.
As preferably, the particle fineness of described mulberry branch, soybean peel, fermentation silkworm faeces is 4 ~ 6 orders.Particle fineness is for 4 ~ 6 orders are to ensure the physico-chemical structure (degree of packing,