CN106718021A - A kind of yield Volvaria volvacea cultivation method high - Google Patents
A kind of yield Volvaria volvacea cultivation method high Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of yield Volvaria volvacea cultivation method high, the straw mushroom cultural method that the present invention is provided is simple and easy to apply, and simple to operate, the straw mushroom of culture is nutritious containing various mineral elements such as iron, zinc, calcium, and yield is high, and DEVELOPMENT PROSPECT is wide.
Description
Technical field
The present invention relates to fungus growing technique field, more particularly to a kind of yield Volvaria volvacea cultivation method high.
Background technology
With the progress of science and technology, mushroom industry fast development, the cultivation two of edible mushroom is constantly increasing thus right
The demand of culturing raw material increases increasingly.In edible mushroom substituting stuff cultivation technology, traditional cultivation raw material mainly have cottonseed, bagasse,
Weed tree sawdust, crop material etc..As people are increasingly mature to the technology that these raw material are comprehensively utilized, the purposes of these raw materials
More extensive, such as wood chip is used to process synthetic plate, and bagasse is used for papermaking etc., this provides for improved Edible Fungi cost, nothing
The pressure of Edible Fungi enterprise, therefore research and development novel edible fungus culturing raw material are increased in shape, product cost is reduced into,
Increase economic efficiency, have great importance.China is the big country of Chinese chestnut plantation, in Chinese chestnut growing area, annual harvesting Chinese chestnut it
Substantial amounts of leftover bits and pieces-Chinese chestnut bud husk can be all produced afterwards.Comprehensive utilizating research and exploitation of the current people to Chinese chestnut bud husk, mostly
The aspects such as solid shape carbon production, tannin extract and nature strength are all concentrated on, because Chinese chestnut bud husk contains carbon, nitrogen and mineral matter etc.
Composition, it is possible to provide the required basic nutrition of edible fungi growth development.
Qin Baoshan exists《The experiment of Chinese chestnut bud husk Xinbao mushroom culturing》With Chinese chestnut bud husk it is main carbon source material in one text, with rice
Grass, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits etc. for auxiliary carbon source material, with rice bran as nitrogen source, set 10 treatment carry out apricot
Abalone mushroom experiment in cultivation, from the commodity property of mycelial growth situation, growth cycle, fructification properties and characteristicses, biological efficiency and mushroom
Etc. aspect be analyzed, as a result show：It is feasible using Chinese chestnut bud husk and Chinese chestnut leaf Xinbao mushroom culturing, individually with Chinese chestnut
Luxuriant shell is for carbon source material or the materials such as appropriate straw, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits are added in Chinese chestnut bud husk,
Obtain preferable cultivation effect.But the tannin in Chinese chestnut bud husk is more, then when flat mushroom and elegant precious mushroom are cultivated, training
Foster yield is not high, and luxuriant shell size must be suitable, and not enough, easy breed bacteria is too big easily to stab for the permeability of too small culture medium
Broken plastic foil, causes humiture not enough, influences the yield of edible mushroom, and experiment consumption above is less, and is only to make
It is the compost of pleurotus eryngii, so Chinese chestnut bud husk must be done into the culture demand that further processing meets multiple edible mushroom, allows
Chinese chestnut bud husk is reasonably utilized, and prevents the wasting of resources, reduces production cost, improves the economic and social benefits.
The content of the invention
The object of the invention is exactly to make up the defect of prior art, there is provided a kind of yield Volvaria volvacea cultivation method high.
The present invention is achieved by the following technical solutions：
A kind of yield Volvaria volvacea cultivation method high, including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put into pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, edible hyphostroma is seeded on culture medium in aseptic operating platform then, in 20-30
Cultivated at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then numerous be to desired quantity through expanding
It is one-level kind；
The Mother culture based formulas are：Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg
White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min,
Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs
Appropriate deionized water is added after mixing dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/
L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to
1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it decocted 2h in being added to deionized water,
Separation of solid and liquid, it is standby after filter residue is dried, old stalk of asparagus is cut into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively
The 24h in 3% limewash is steeped, taking-up drains rear and Chinese chestnut bud husk extract solution, soya-bean cake, brewer's yeast, calcium acetate and citric acid waste
Processed according to half sponge process, primary fermentation 18 days, altogether turning 4 times, then overlay film fermentation, keeps 10h at 60 DEG C, kept at 50 DEG C
4 days, make the water content of compost between 60-65% after fermentation ends, pH value is 7-7.5；
The formula of the compost is matched according to mass percent：Chinese chestnut bud husk 55-70%, old stalk of asparagus 20-30%, soya-bean cake
22-26%, brewer's yeast 1.7-3%, calcium acetate 1-1.5%, citric acid waste 0.7-2%, appropriate deionized water；
B, by sodium alginate according to mass volume ratio 1:10 are dissolved in deionized water, by two after sodium metaperiodate deionized water dissolving
Person mixes, and lucifuge stirring 24h, adds ethylene glycol terminating reaction 15-20min equimolar with sodium metaperiodate, thoroughly at room temperature
Analysis removes unreacted material in 3 days, changes water 2-3 times daily, and dialysis is standby by dialyzate freeze-drying after terminating；It is again that gelatin is molten
In 40% acetum, it is stirring evenly and then adding into above-mentioned standby dialyzate and obtains mixed solution, electricity is added to after stirring
Spinning is carried out in frame, 12h in pH is 9 90% ethanol solution, drying for standby after taking-up is then immersed in；
C, the material for preparing step a and step b are according to mass ratio 20：1 mixing and stirring, then using the poly- of appropriate gauge
The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, and sterilizing, 126 in high-pressure sterilizing pot are placed in after tightening sack
DEG C 2-2.5h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into training
In nutriment, according still further to sterile working inoculation, inoculum concentration is advisable with strain covering charge level；
Described nutrient solution prescription is：Sodium selenite 4-5ppm, ammonium molybdate 6-8ppm, zinc sulfate 6-8ppm, calcium dihydrogen phosphate 4-
6ppm, ferrous sulfate 7-9ppm, KI 4-5ppm, add water to 1000mL；
D, the culturing room for postvaccinal culture bag being placed into dry, cleaning, being divulged information carry out half-light culture, and temperature control is in 18-
28 DEG C, relative air humidity is 65-75%, and a culture bag was turned over every 7-10 days, and mushroom house is produced after mycelia purseful long；
E, selection water quality it is good, quality is loose, the soil without miscellaneous worm's ovum, add 2-3% volcanic rock, the attapulgite and 5-8% of 3-5%
Deer natural pond soil mixing and stirring, then add 0.1% urea, 0.1% phosphate fertilizer, 1.5% lime and 0.1% carbendazim,
It is mixed into Nutrition Soil, and overlay film boring cover 2-3 days, the humidity for keeping Nutrition Soil is 45-55%, then by the bacterium in step d
Bag carries out vallum earthing, and the condition needed when then according to edible mushroom controls suitable humiture and ventilation condition.
It is an advantage of the invention that：Chinese chestnut bud husk is spherical, the close involucre being needled in Castanea mollissima Nut outside, due to edible mushroom
Become adult come edible good protein by decomposing, converting the organic matter for going out of use, and Chinese chestnut bud husk contains carbon, nitrogen and ore deposit
The compositions such as material, can be as the required basic nutrition of edible fungi growth development, so the present invention is by after Chinese chestnut bud husk and liquor
Chinese chestnut bud husk slag as main charcoal source material, the juice of Chinese chestnut bud husk is used as zymotic fluid, then is aided with old stalk of asparagus, soya-bean cake, beer
Brewer yeast, calcium acetate and citric acid waste meet straw mushroom growth to carbon source, nitrogen source and other nutrition as the compost of edible mushroom
Demand, and gelatin/oxidized sodium alginate cross filament is prepared by chemical reaction, ease up with good water suction, water conservation
Performance is released, nutrient solution edible mushroom, drought-resistant and fertilizer can be in time supplied to, it is ensured that the supply and demand of its nutrition, makes its hair bacterium fast, wire vent
It is many, and reduce the later stage cultivate straw mushroom manual operation, time saving and energy saving, the mushroom that finally will appear from former base carries out vallum plantation,
Homemade Nutrition Soil is covered, mushrooms can be plucked in many batches of later stage and be provided nutrition for it, make that its mushroom growing up number is more, yield is high, and
And be the resource huge profit of Chinese chestnut bud husk with new approach is found, reduce the pollution to environment, the straw mushroom culture side that the present invention is provided
Method is simple and easy to apply, simple to operate, and the straw mushroom of culture is nutritious containing various mineral elements such as iron, zinc, calcium, and yield is high, exploitation
Have a extensive future.
Specific embodiment
A kind of yield Volvaria volvacea cultivation method high, including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put into pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, edible hyphostroma is seeded on culture medium in aseptic operating platform then, at 20 DEG C
At a temperature of cultivate, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then numerous be to desired quantity through expanding
One-level kind；
The Mother culture based formulas are：Potato mixed juice 200g/L, agar 12g/L, sucrose 20g/L, peptone 5g/L, work
Property charcoal 0.3g/L, adds water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30min, filter
Residue, merging filtrate is gone simultaneously to add dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, be sufficiently stirred for
Appropriate deionized water is added after dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160g/L, Chinese chestnut bud husk 40g/L, dusty yeast 5g/L, glucose
20g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it decocted 2h in being added to deionized water,
Separation of solid and liquid, it is standby after filter residue is dried, old stalk of asparagus is cut into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively
The 24h in 3% limewash is steeped, taking-up drains rear and Chinese chestnut bud husk extract solution, soya-bean cake, brewer's yeast, calcium acetate and citric acid waste
Processed according to half sponge process, primary fermentation 18 days, altogether turning 4 times, then overlay film fermentation, keeps 10h at 60 DEG C, kept at 50 DEG C
4 days, make the water content of compost between 60% after fermentation ends, pH value is 7；
The formula of the compost is matched according to mass percent：Chinese chestnut bud husk 55%, old stalk of asparagus 20%, soya-bean cake 22%, beer
Yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%, appropriate deionized water；
B, by sodium alginate according to mass volume ratio 1:10 are dissolved in deionized water, by two after sodium metaperiodate deionized water dissolving
Person mixes, at room temperature lucifuge stirring 24h, adds ethylene glycol terminating reaction 15min equimolar with sodium metaperiodate, dialysis 3
It removes unreacted material, water is changed daily 2 times, and dialysis is standby by dialyzate freeze-drying after terminating；Gelatin is dissolved in 40% again
Acetum in, be stirring evenly and then adding into above-mentioned standby dialyzate and obtain mixed solution, be added to electric spinning machine after stirring
In carry out spinning, be then immersed in 12h in pH is 9 90% ethanol solution, drying for standby after taking-up；
C, the material for preparing step a and step b are according to mass ratio 20：1 mixing and stirring, then using the poly- of appropriate gauge
The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, and sterilizing, 126 in high-pressure sterilizing pot are placed in after tightening sack
DEG C 2h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into compost
In, according still further to sterile working inoculation, inoculum concentration is advisable with strain covering charge level；
Described nutrient solution prescription is：Sodium selenite 4ppm, ammonium molybdate 6ppm, zinc sulfate 6ppm, calcium dihydrogen phosphate 4ppm, sulfuric acid
Ferrous 7ppm, KI 4ppm, add water to 1000mL；
D, the culturing room for postvaccinal culture bag being placed into dry, cleaning, being divulged information carry out half-light culture, and temperature control is 18
DEG C, relative air humidity is 65%, and a culture bag was turned over every 7 days, and mushroom house is produced after mycelia purseful long；
E, selection water quality is good, quality is loose, the soil without miscellaneous worm's ovum, adds 2% volcanic rock, 3% attapulgite and 5% deer natural pond
Native mixing and stirring, then adds 0.1% urea, 0.1% phosphate fertilizer, 1.5% lime and 0.1% carbendazim, and mixing is stirred
Mix Nutrition Soil, and overlay film boring cover 2 days, the humidity for keeping Nutrition Soil is 45%, and the bacterium bag in step d then is carried out into vallum
Earthing, the condition needed when then according to edible mushroom controls suitable humiture and ventilation condition.
The upgrowth situation of hypha of edible fungus is compared in order to embody Chinese chestnut bud husk, different cultures are prepared according to following formula
Material is cultivated straw mushroom, and the indices to growth course carry out observation comparing
A Chinese chestnut bud husks 55%, old stalk of asparagus 20%, soya-bean cake 22%, brewer's yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%
B glucose 55%, old stalk of asparagus 20%, soya-bean cake 22%, brewer's yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%
C Chinese chestnut bud husks 75%, soya-bean cake 22%, brewer's yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%
D glucose 75%, soya-bean cake 22%, brewer's yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%
The mycelial growth situation of control
Speed long（mm/d）Mycelial density mycelia thickness mycelia color and luster
A 3.57 +++ ++ it is relatively thick dense white
B 3.03 ++++thin dense white
C 3.24 ++++thick dense white
D 2.95 +++ it is relatively thin white
"+" number represents mycelial density, and "+" represents that density is bigger
Growth cycle compares
There is former base（d）Fructification grows up to（d）Growth cycle（d）
A 7 7 82
B 8 8.5 87
C 7 7.5 85
D 9 9 92
The properties and characteristicses of each formula fructification
The mono- mushroom weight/g biological conversion rates/% of stem diameter/cm stems length/cm
A 1.5 7.7 29.12 31.8
B 1.1 6.3 24.54 23.7
C 1.3 7.2 26.41 26.8
D 0.9 5.7 22.34 21.5
Claims (1)
1. a kind of yield Volvaria volvacea cultivation method high, it is characterised in that including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put into pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, edible hyphostroma is seeded on culture medium in aseptic operating platform then, in 20-30
Cultivated at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then numerous be to desired quantity through expanding
It is one-level kind；
The Mother culture based formulas are：Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg
White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min,
Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs
Appropriate deionized water is added after mixing dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/
L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to
1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it decocted 2h in being added to deionized water,
Separation of solid and liquid, it is standby after filter residue is dried, old stalk of asparagus is cut into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively
The 24h in 3% limewash is steeped, taking-up drains rear and Chinese chestnut bud husk extract solution, soya-bean cake, brewer's yeast, calcium acetate and citric acid waste
Processed according to half sponge process, primary fermentation 18 days, altogether turning 4 times, then overlay film fermentation, keeps 10h at 60 DEG C, kept at 50 DEG C
4 days, make the water content of compost between 60-65% after fermentation ends, pH value is 7-7.5；
The formula of the compost is matched according to mass percent：Chinese chestnut bud husk 55-70%, old stalk of asparagus 30-40%, soya-bean cake 4-
6%th, brewer's yeast 1.7-3%, calcium acetate 1-1.5%, citric acid waste 0.7-2%, appropriate deionized water；
B, by sodium alginate according to mass volume ratio 1:10 are dissolved in deionized water, by two after sodium metaperiodate deionized water dissolving
Person mixes, and lucifuge stirring 24h, adds ethylene glycol terminating reaction 15-20min equimolar with sodium metaperiodate, thoroughly at room temperature
Analysis removes unreacted material in 3 days, changes water 2-3 times daily, and dialysis is standby by dialyzate freeze-drying after terminating；It is again that gelatin is molten
In 40% acetum, it is stirring evenly and then adding into above-mentioned standby dialyzate and obtains mixed solution, electricity is added to after stirring
Spinning is carried out in frame, 12h in pH is 9 90% ethanol solution, drying for standby after taking-up is then immersed in；
C, the material for preparing step a and step b are according to mass ratio 20：1 mixing and stirring, then using the poly- of appropriate gauge
The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, and sterilizing, 126 in high-pressure sterilizing pot are placed in after tightening sack
DEG C 2-2.5h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into training
In nutriment, according still further to sterile working inoculation, inoculum concentration is advisable with strain covering charge level；
Described nutrient solution prescription is：Sodium selenite 4-5ppm, ammonium molybdate 6-8ppm, zinc sulfate 6-8ppm, calcium dihydrogen phosphate 4-
6ppm, ferrous sulfate 7-9ppm, KI 4-5ppm, add water to 1000mL；
D, the culturing room for postvaccinal culture bag being placed into dry, cleaning, being divulged information carry out half-light culture, and temperature control is in 18-
28 DEG C, relative air humidity is 65-75%, and a culture bag was turned over every 7-10 days, and mushroom house is produced after mycelia purseful long；
E, selection water quality it is good, quality is loose, the soil without miscellaneous worm's ovum, add 2-3% volcanic rock, the attapulgite and 5-8% of 3-5%
Deer natural pond soil mixing and stirring, then add 0.1% urea, 0.1% phosphate fertilizer, 1.5% lime and 0.1% carbendazim,
It is mixed into Nutrition Soil, and overlay film boring cover 2-3 days, the humidity for keeping Nutrition Soil is 45-55%, then by the bacterium in step d
Bag carries out vallum earthing, and the condition needed when then according to edible mushroom controls suitable humiture and ventilation condition.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107509530A (en) * | 2017-09-19 | 2017-12-26 | 迁西县林中宝生物科技有限公司 | A kind of big bacteria stick method that earthing fixed point fruiting does not cultivate chestnut mushroom |
| CN107950288A (en) * | 2017-11-20 | 2018-04-24 | 山东省农业科学院农业资源与环境研究所 | A kind of planting technique of straw mushroom |
| CN108812054A (en) * | 2018-05-30 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A method of cultivation Hericium erinaceus |
| CN110214626A (en) * | 2019-07-16 | 2019-09-10 | 灌南县人民政府蔬菜办公室 | A kind of straw mushroom cultural method |
| CN110476704A (en) * | 2019-09-25 | 2019-11-22 | 贵州山环菌草科技有限公司 | A kind of Volvaria volvacea cultivation method |
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| CN108812054B (en) * | 2018-05-30 | 2020-06-09 | 江苏圣福来生态农业有限公司 | Method for cultivating hericium erinaceus |
| CN110214626A (en) * | 2019-07-16 | 2019-09-10 | 灌南县人民政府蔬菜办公室 | A kind of straw mushroom cultural method |
| CN110214626B (en) * | 2019-07-16 | 2021-08-10 | 灌南县人民政府蔬菜办公室 | Straw mushroom culture method |
| CN110476704A (en) * | 2019-09-25 | 2019-11-22 | 贵州山环菌草科技有限公司 | A kind of Volvaria volvacea cultivation method |
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