CN106718021A - A kind of yield Volvaria volvacea cultivation method high - Google Patents
A kind of yield Volvaria volvacea cultivation method high Download PDFInfo
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- CN106718021A CN106718021A CN201611044375.3A CN201611044375A CN106718021A CN 106718021 A CN106718021 A CN 106718021A CN 201611044375 A CN201611044375 A CN 201611044375A CN 106718021 A CN106718021 A CN 106718021A
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- deionized water
- chinese chestnut
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 47
- 235000018244 Castanea mollissima Nutrition 0.000 claims description 44
- 235000006667 Aleurites moluccana Nutrition 0.000 claims description 43
- 239000010903 husk Substances 0.000 claims description 37
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 33
- 239000008367 deionised water Substances 0.000 claims description 27
- 230000001954 sterilising Effects 0.000 claims description 18
- 238000003756 stirring Methods 0.000 claims description 18
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 17
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 16
- 239000001963 growth media Substances 0.000 claims description 16
- JQWHASGSAFIOCM-UHFFFAOYSA-M Sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L mgso4 Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- 235000016709 nutrition Nutrition 0.000 claims description 12
- 230000035764 nutrition Effects 0.000 claims description 12
- 239000002689 soil Substances 0.000 claims description 12
- VSGNNIFQASZAOI-UHFFFAOYSA-L Calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 11
- 240000007842 Glycine max Species 0.000 claims description 11
- 235000010469 Glycine max Nutrition 0.000 claims description 11
- 235000012970 cakes Nutrition 0.000 claims description 11
- 235000011092 calcium acetate Nutrition 0.000 claims description 11
- 239000001639 calcium acetate Substances 0.000 claims description 11
- 229960005147 calcium acetate Drugs 0.000 claims description 11
- 239000002699 waste material Substances 0.000 claims description 11
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 240000001498 Asparagus officinalis Species 0.000 claims description 9
- 235000005340 Asparagus officinalis Nutrition 0.000 claims description 9
- 240000001016 Solanum tuberosum Species 0.000 claims description 9
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 9
- 239000002361 compost Substances 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 9
- 230000004151 fermentation Effects 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N D-sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M Monopotassium phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- CZMRCDWAGMRECN-GDQSFJPYSA-N Sucrose Natural products O([C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1)[C@@]1(CO)[C@H](O)[C@@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-GDQSFJPYSA-N 0.000 claims description 6
- 229960000344 Thiamine hydrochloride Drugs 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 235000005824 corn Nutrition 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 235000013312 flour Nutrition 0.000 claims description 6
- LYCAIKOWRPUZTN-UHFFFAOYSA-N glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 235000019190 thiamine hydrochloride Nutrition 0.000 claims description 6
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 6
- 239000011747 thiamine hydrochloride Substances 0.000 claims description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 108010010803 Gelatin Proteins 0.000 claims description 4
- 229940005550 Sodium alginate Drugs 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000015165 citric acid Nutrition 0.000 claims description 4
- 238000000502 dialysis Methods 0.000 claims description 4
- 239000008273 gelatin Substances 0.000 claims description 4
- 229920000159 gelatin Polymers 0.000 claims description 4
- 235000019322 gelatine Nutrition 0.000 claims description 4
- 235000011852 gelatine desserts Nutrition 0.000 claims description 4
- MSXHSNHNTORCAW-UHFFFAOYSA-M sodium 3,4,5,6-tetrahydroxyoxane-2-carboxylate Chemical compound [Na+].OC1OC(C([O-])=O)C(O)C(O)C1O MSXHSNHNTORCAW-UHFFFAOYSA-M 0.000 claims description 4
- 239000000661 sodium alginate Substances 0.000 claims description 4
- 235000010413 sodium alginate Nutrition 0.000 claims description 4
- 238000009987 spinning Methods 0.000 claims description 4
- 229920002972 Acrylic fiber Polymers 0.000 claims description 3
- QGAVSDVURUSLQK-UHFFFAOYSA-N Ammonium heptamolybdate Chemical compound N.N.N.N.N.N.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.[Mo].[Mo].[Mo].[Mo].[Mo].[Mo].[Mo] QGAVSDVURUSLQK-UHFFFAOYSA-N 0.000 claims description 3
- 229940062672 CALCIUM DIHYDROGEN PHOSPHATE Drugs 0.000 claims description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N Carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 3
- 241000282994 Cervidae Species 0.000 claims description 3
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 3
- 240000008790 Musa x paradisiaca Species 0.000 claims description 3
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 3
- 210000004681 Ovum Anatomy 0.000 claims description 3
- BVTBRVFYZUCAKH-UHFFFAOYSA-L Sodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 3
- 235000015450 Tilia cordata Nutrition 0.000 claims description 3
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L Zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 239000011609 ammonium molybdate Substances 0.000 claims description 3
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 3
- 229940010552 ammonium molybdate Drugs 0.000 claims description 3
- 229960000892 attapulgite Drugs 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 3
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 239000006013 carbendazim Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 235000001727 glucose Nutrition 0.000 claims description 3
- 238000001802 infusion Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000004571 lime Substances 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 239000002609 media Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 3
- 229910052625 palygorskite Inorganic materials 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- 239000002686 phosphate fertilizer Substances 0.000 claims description 3
- 235000012015 potatoes Nutrition 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000011435 rock Substances 0.000 claims description 3
- 239000011781 sodium selenite Substances 0.000 claims description 3
- 235000015921 sodium selenite Nutrition 0.000 claims description 3
- 229960001471 sodium selenite Drugs 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N β-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L Iron(II) sulfate Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 238000004458 analytical method Methods 0.000 claims description 2
- 235000014103 egg white Nutrition 0.000 claims description 2
- 210000000969 egg white Anatomy 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims description 2
- 239000011790 ferrous sulphate Substances 0.000 claims description 2
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- 244000136475 Aleurites moluccana Species 0.000 claims 6
- 240000006794 Volvariella volvacea Species 0.000 abstract description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 2
- 239000011575 calcium Substances 0.000 abstract description 2
- 229910052791 calcium Inorganic materials 0.000 abstract description 2
- 229910052742 iron Inorganic materials 0.000 abstract description 2
- 239000002366 mineral element Substances 0.000 abstract description 2
- 235000008935 nutritious Nutrition 0.000 abstract description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 abstract description 2
- 239000011701 zinc Substances 0.000 abstract description 2
- 229910052725 zinc Inorganic materials 0.000 abstract description 2
- 240000004957 Castanea mollissima Species 0.000 description 38
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 241000233866 Fungi Species 0.000 description 7
- 229910052799 carbon Inorganic materials 0.000 description 7
- 239000002994 raw material Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 241000609240 Ambelania acida Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- VXMKYRQZQXVKGB-CWWHNZPOSA-N Tannin Chemical compound O([C@H]1[C@H]([C@@H]2OC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)O[C@H]([C@H]2O)O1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 VXMKYRQZQXVKGB-CWWHNZPOSA-N 0.000 description 2
- 241000209149 Zea Species 0.000 description 2
- 239000010905 bagasse Substances 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920001864 tannin Polymers 0.000 description 2
- 235000018553 tannin Nutrition 0.000 description 2
- 239000001648 tannin Substances 0.000 description 2
- 229960005069 Calcium Drugs 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001489755 Haliotis tuberculata Species 0.000 description 1
- 229960003284 Iron Drugs 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000004928 Paspalum scrobiculatum Species 0.000 description 1
- 235000003675 Paspalum scrobiculatum Nutrition 0.000 description 1
- 240000004247 Pleurotus eryngii Species 0.000 description 1
- 235000001681 Pleurotus eryngii Nutrition 0.000 description 1
- 240000005204 Prunus armeniaca Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 229940046282 Zinc Drugs 0.000 description 1
- 229940091251 Zinc Supplements Drugs 0.000 description 1
- 230000001058 adult Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention discloses a kind of yield Volvaria volvacea cultivation method high, the straw mushroom cultural method that the present invention is provided is simple and easy to apply, and simple to operate, the straw mushroom of culture is nutritious containing various mineral elements such as iron, zinc, calcium, and yield is high, and DEVELOPMENT PROSPECT is wide.
Description
Technical field
The present invention relates to fungus growing technique field, more particularly to a kind of yield Volvaria volvacea cultivation method high.
Background technology
With the progress of science and technology, mushroom industry fast development, the cultivation two of edible mushroom is constantly increasing thus right
The demand of culturing raw material increases increasingly.In edible mushroom substituting stuff cultivation technology, traditional cultivation raw material mainly have cottonseed, bagasse,
Weed tree sawdust, crop material etc..As people are increasingly mature to the technology that these raw material are comprehensively utilized, the purposes of these raw materials
More extensive, such as wood chip is used to process synthetic plate, and bagasse is used for papermaking etc., this provides for improved Edible Fungi cost, nothing
The pressure of Edible Fungi enterprise, therefore research and development novel edible fungus culturing raw material are increased in shape, product cost is reduced into,
Increase economic efficiency, have great importance.China is the big country of Chinese chestnut plantation, in Chinese chestnut growing area, annual harvesting Chinese chestnut it
Substantial amounts of leftover bits and pieces-Chinese chestnut bud husk can be all produced afterwards.Comprehensive utilizating research and exploitation of the current people to Chinese chestnut bud husk, mostly
The aspects such as solid shape carbon production, tannin extract and nature strength are all concentrated on, because Chinese chestnut bud husk contains carbon, nitrogen and mineral matter etc.
Composition, it is possible to provide the required basic nutrition of edible fungi growth development.
Qin Baoshan exists《The experiment of Chinese chestnut bud husk Xinbao mushroom culturing》With Chinese chestnut bud husk it is main carbon source material in one text, with rice
Grass, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits etc. for auxiliary carbon source material, with rice bran as nitrogen source, set 10 treatment carry out apricot
Abalone mushroom experiment in cultivation, from the commodity property of mycelial growth situation, growth cycle, fructification properties and characteristicses, biological efficiency and mushroom
Etc. aspect be analyzed, as a result show：It is feasible using Chinese chestnut bud husk and Chinese chestnut leaf Xinbao mushroom culturing, individually with Chinese chestnut
Luxuriant shell is for carbon source material or the materials such as appropriate straw, miscellaneous tree leaf, Chinese chestnut leaf and ramulus mori bits are added in Chinese chestnut bud husk,
Obtain preferable cultivation effect.But the tannin in Chinese chestnut bud husk is more, then when flat mushroom and elegant precious mushroom are cultivated, training
Foster yield is not high, and luxuriant shell size must be suitable, and not enough, easy breed bacteria is too big easily to stab for the permeability of too small culture medium
Broken plastic foil, causes humiture not enough, influences the yield of edible mushroom, and experiment consumption above is less, and is only to make
It is the compost of pleurotus eryngii, so Chinese chestnut bud husk must be done into the culture demand that further processing meets multiple edible mushroom, allows
Chinese chestnut bud husk is reasonably utilized, and prevents the wasting of resources, reduces production cost, improves the economic and social benefits.
The content of the invention
The object of the invention is exactly to make up the defect of prior art, there is provided a kind of yield Volvaria volvacea cultivation method high.
The present invention is achieved by the following technical solutions：
A kind of yield Volvaria volvacea cultivation method high, including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put into pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, edible hyphostroma is seeded on culture medium in aseptic operating platform then, in 20-30
Cultivated at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then numerous be to desired quantity through expanding
It is one-level kind；
The Mother culture based formulas are：Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg
White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min,
Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs
Appropriate deionized water is added after mixing dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/
L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to
1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it decocted 2h in being added to deionized water,
Separation of solid and liquid, it is standby after filter residue is dried, old stalk of asparagus is cut into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively
The 24h in 3% limewash is steeped, taking-up drains rear and Chinese chestnut bud husk extract solution, soya-bean cake, brewer's yeast, calcium acetate and citric acid waste
Processed according to half sponge process, primary fermentation 18 days, altogether turning 4 times, then overlay film fermentation, keeps 10h at 60 DEG C, kept at 50 DEG C
4 days, make the water content of compost between 60-65% after fermentation ends, pH value is 7-7.5；
The formula of the compost is matched according to mass percent：Chinese chestnut bud husk 55-70%, old stalk of asparagus 20-30%, soya-bean cake
22-26%, brewer's yeast 1.7-3%, calcium acetate 1-1.5%, citric acid waste 0.7-2%, appropriate deionized water；
B, by sodium alginate according to mass volume ratio 1:10 are dissolved in deionized water, by two after sodium metaperiodate deionized water dissolving
Person mixes, and lucifuge stirring 24h, adds ethylene glycol terminating reaction 15-20min equimolar with sodium metaperiodate, thoroughly at room temperature
Analysis removes unreacted material in 3 days, changes water 2-3 times daily, and dialysis is standby by dialyzate freeze-drying after terminating；It is again that gelatin is molten
In 40% acetum, it is stirring evenly and then adding into above-mentioned standby dialyzate and obtains mixed solution, electricity is added to after stirring
Spinning is carried out in frame, 12h in pH is 9 90% ethanol solution, drying for standby after taking-up is then immersed in；
C, the material for preparing step a and step b are according to mass ratio 20：1 mixing and stirring, then using the poly- of appropriate gauge
The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, and sterilizing, 126 in high-pressure sterilizing pot are placed in after tightening sack
DEG C 2-2.5h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into training
In nutriment, according still further to sterile working inoculation, inoculum concentration is advisable with strain covering charge level；
Described nutrient solution prescription is：Sodium selenite 4-5ppm, ammonium molybdate 6-8ppm, zinc sulfate 6-8ppm, calcium dihydrogen phosphate 4-
6ppm, ferrous sulfate 7-9ppm, KI 4-5ppm, add water to 1000mL；
D, the culturing room for postvaccinal culture bag being placed into dry, cleaning, being divulged information carry out half-light culture, and temperature control is in 18-
28 DEG C, relative air humidity is 65-75%, and a culture bag was turned over every 7-10 days, and mushroom house is produced after mycelia purseful long；
E, selection water quality it is good, quality is loose, the soil without miscellaneous worm's ovum, add 2-3% volcanic rock, the attapulgite and 5-8% of 3-5%
Deer natural pond soil mixing and stirring, then add 0.1% urea, 0.1% phosphate fertilizer, 1.5% lime and 0.1% carbendazim,
It is mixed into Nutrition Soil, and overlay film boring cover 2-3 days, the humidity for keeping Nutrition Soil is 45-55%, then by the bacterium in step d
Bag carries out vallum earthing, and the condition needed when then according to edible mushroom controls suitable humiture and ventilation condition.
It is an advantage of the invention that：Chinese chestnut bud husk is spherical, the close involucre being needled in Castanea mollissima Nut outside, due to edible mushroom
Become adult come edible good protein by decomposing, converting the organic matter for going out of use, and Chinese chestnut bud husk contains carbon, nitrogen and ore deposit
The compositions such as material, can be as the required basic nutrition of edible fungi growth development, so the present invention is by after Chinese chestnut bud husk and liquor
Chinese chestnut bud husk slag as main charcoal source material, the juice of Chinese chestnut bud husk is used as zymotic fluid, then is aided with old stalk of asparagus, soya-bean cake, beer
Brewer yeast, calcium acetate and citric acid waste meet straw mushroom growth to carbon source, nitrogen source and other nutrition as the compost of edible mushroom
Demand, and gelatin/oxidized sodium alginate cross filament is prepared by chemical reaction, ease up with good water suction, water conservation
Performance is released, nutrient solution edible mushroom, drought-resistant and fertilizer can be in time supplied to, it is ensured that the supply and demand of its nutrition, makes its hair bacterium fast, wire vent
It is many, and reduce the later stage cultivate straw mushroom manual operation, time saving and energy saving, the mushroom that finally will appear from former base carries out vallum plantation,
Homemade Nutrition Soil is covered, mushrooms can be plucked in many batches of later stage and be provided nutrition for it, make that its mushroom growing up number is more, yield is high, and
And be the resource huge profit of Chinese chestnut bud husk with new approach is found, reduce the pollution to environment, the straw mushroom culture side that the present invention is provided
Method is simple and easy to apply, simple to operate, and the straw mushroom of culture is nutritious containing various mineral elements such as iron, zinc, calcium, and yield is high, exploitation
Have a extensive future.
Specific embodiment
A kind of yield Volvaria volvacea cultivation method high, including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put into pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, edible hyphostroma is seeded on culture medium in aseptic operating platform then, at 20 DEG C
At a temperature of cultivate, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then numerous be to desired quantity through expanding
One-level kind；
The Mother culture based formulas are：Potato mixed juice 200g/L, agar 12g/L, sucrose 20g/L, peptone 5g/L, work
Property charcoal 0.3g/L, adds water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30min, filter
Residue, merging filtrate is gone simultaneously to add dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, be sufficiently stirred for
Appropriate deionized water is added after dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160g/L, Chinese chestnut bud husk 40g/L, dusty yeast 5g/L, glucose
20g/L, magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1g/L, Thiamine Hydrochloride Tablets two panels/L, add water to 1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it decocted 2h in being added to deionized water,
Separation of solid and liquid, it is standby after filter residue is dried, old stalk of asparagus is cut into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively
The 24h in 3% limewash is steeped, taking-up drains rear and Chinese chestnut bud husk extract solution, soya-bean cake, brewer's yeast, calcium acetate and citric acid waste
Processed according to half sponge process, primary fermentation 18 days, altogether turning 4 times, then overlay film fermentation, keeps 10h at 60 DEG C, kept at 50 DEG C
4 days, make the water content of compost between 60% after fermentation ends, pH value is 7；
The formula of the compost is matched according to mass percent：Chinese chestnut bud husk 55%, old stalk of asparagus 20%, soya-bean cake 22%, beer
Yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%, appropriate deionized water；
B, by sodium alginate according to mass volume ratio 1:10 are dissolved in deionized water, by two after sodium metaperiodate deionized water dissolving
Person mixes, at room temperature lucifuge stirring 24h, adds ethylene glycol terminating reaction 15min equimolar with sodium metaperiodate, dialysis 3
It removes unreacted material, water is changed daily 2 times, and dialysis is standby by dialyzate freeze-drying after terminating；Gelatin is dissolved in 40% again
Acetum in, be stirring evenly and then adding into above-mentioned standby dialyzate and obtain mixed solution, be added to electric spinning machine after stirring
In carry out spinning, be then immersed in 12h in pH is 9 90% ethanol solution, drying for standby after taking-up；
C, the material for preparing step a and step b are according to mass ratio 20：1 mixing and stirring, then using the poly- of appropriate gauge
The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, and sterilizing, 126 in high-pressure sterilizing pot are placed in after tightening sack
DEG C 2h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into compost
In, according still further to sterile working inoculation, inoculum concentration is advisable with strain covering charge level；
Described nutrient solution prescription is：Sodium selenite 4ppm, ammonium molybdate 6ppm, zinc sulfate 6ppm, calcium dihydrogen phosphate 4ppm, sulfuric acid
Ferrous 7ppm, KI 4ppm, add water to 1000mL；
D, the culturing room for postvaccinal culture bag being placed into dry, cleaning, being divulged information carry out half-light culture, and temperature control is 18
DEG C, relative air humidity is 65%, and a culture bag was turned over every 7 days, and mushroom house is produced after mycelia purseful long；
E, selection water quality is good, quality is loose, the soil without miscellaneous worm's ovum, adds 2% volcanic rock, 3% attapulgite and 5% deer natural pond
Native mixing and stirring, then adds 0.1% urea, 0.1% phosphate fertilizer, 1.5% lime and 0.1% carbendazim, and mixing is stirred
Mix Nutrition Soil, and overlay film boring cover 2 days, the humidity for keeping Nutrition Soil is 45%, and the bacterium bag in step d then is carried out into vallum
Earthing, the condition needed when then according to edible mushroom controls suitable humiture and ventilation condition.
The upgrowth situation of hypha of edible fungus is compared in order to embody Chinese chestnut bud husk, different cultures are prepared according to following formula
Material is cultivated straw mushroom, and the indices to growth course carry out observation comparing
A Chinese chestnut bud husks 55%, old stalk of asparagus 20%, soya-bean cake 22%, brewer's yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%
B glucose 55%, old stalk of asparagus 20%, soya-bean cake 22%, brewer's yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%
C Chinese chestnut bud husks 75%, soya-bean cake 22%, brewer's yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%
D glucose 75%, soya-bean cake 22%, brewer's yeast 1.7%, calcium acetate 1%, citric acid waste 0.7%
The mycelial growth situation of control
Speed long（mm/d）Mycelial density mycelia thickness mycelia color and luster
A 3.57 +++ ++ it is relatively thick dense white
B 3.03 ++++thin dense white
C 3.24 ++++thick dense white
D 2.95 +++ it is relatively thin white
"+" number represents mycelial density, and "+" represents that density is bigger
Growth cycle compares
There is former base（d）Fructification grows up to（d）Growth cycle（d）
A 7 7 82
B 8 8.5 87
C 7 7.5 85
D 9 9 92
The properties and characteristicses of each formula fructification
The mono- mushroom weight/g biological conversion rates/% of stem diameter/cm stems length/cm
A 1.5 7.7 29.12 31.8
B 1.1 6.3 24.54 23.7
C 1.3 7.2 26.41 26.8
D 0.9 5.7 22.34 21.5
Claims (1)
1. a kind of yield Volvaria volvacea cultivation method high, it is characterised in that including step in detail below：
（1）The separation and culture of edible mushroom parent species：
Peeling potatoes section, the peeling section of rotten banana are put into pot, according to mass volume ratio 1g：5ml adds deionized water
Potato is boiled to thoroughly well cooked but not mushy, residue is filtered off, filtrate is put back in pot, add agar, sucrose, activated carbon and peptone, fully
Appropriate deionized water is added after stirring and dissolving, is sub-packed in test tube while hot, be placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
30min, takes out after cooling and is put into inclined-plane, edible hyphostroma is seeded on culture medium in aseptic operating platform then, in 20-30
Cultivated at a temperature of DEG C, until mycelia covers with medium slant, that is, obtain edible mushroom parent species, then numerous be to desired quantity through expanding
It is one-level kind；
The Mother culture based formulas are：Potato mixed juice 200-220g/L, agar 12-15g/L, sucrose 20-25g/L, egg
White peptone 5-7g/L, activated carbon 0.3-0.5g/L, add water to 1000mL；
（2）The preparation and culture of liquid strain culture medium：
Chinese chestnut bud husk pulverizer was crushed into 60 mesh sieves, then with deionized water according to mass ratio 1:15 are blended in bath temperature
Infusion 2h at 100 DEG C, filters off residue, by corn flour and wheat bran according to mass ratio 3:2 are added in deionized water and boil 30-40min,
Residue is filtered off, merging filtrate simultaneously adds dusty yeast, glucose, magnesium sulfate, potassium dihydrogen phosphate and Thiamine Hydrochloride Tablets two panels, fully stirs
Appropriate deionized water is added after mixing dissolving, is sub-packed in container while hot, be subsequently placed in high-pressure sterilizing pot in 121 DEG C of sterilizings
40min, is cooled to standby after room temperature；Liquid is inoculated on aseptic operating platform according to 0.2mm 0.2mm sizes with first class inoculum
In culture medium, it is placed in 25 DEG C of shaking table and is cultivated with 180r/min, when mycelium pellet covers with 80% culture medium, you can
Solid culture medium is inoculated with；
The Liquid Culture based formulas are：Corn flour mixed juice 160-200g/L, Chinese chestnut bud husk 40-50g/L, dusty yeast 5-8g/
L, glucose 20-25g/L, magnesium sulfate 0.5-0.8g/L, potassium dihydrogen phosphate 1-1.4g/L, Thiamine Hydrochloride Tablets two panels/L, add water to
1000mL；
（3）Cultivation fruiting：
A, Chinese chestnut bud husk was crushed 60 mesh sieves, was divided into two according to weight ratio, a copy of it decocted 2h in being added to deionized water,
Separation of solid and liquid, it is standby after filter residue is dried, old stalk of asparagus is cut into 20cm sizes, and filter residue, remaining Chinese chestnut bud husk soak respectively
The 24h in 3% limewash is steeped, taking-up drains rear and Chinese chestnut bud husk extract solution, soya-bean cake, brewer's yeast, calcium acetate and citric acid waste
Processed according to half sponge process, primary fermentation 18 days, altogether turning 4 times, then overlay film fermentation, keeps 10h at 60 DEG C, kept at 50 DEG C
4 days, make the water content of compost between 60-65% after fermentation ends, pH value is 7-7.5；
The formula of the compost is matched according to mass percent：Chinese chestnut bud husk 55-70%, old stalk of asparagus 30-40%, soya-bean cake 4-
6%th, brewer's yeast 1.7-3%, calcium acetate 1-1.5%, citric acid waste 0.7-2%, appropriate deionized water；
B, by sodium alginate according to mass volume ratio 1:10 are dissolved in deionized water, by two after sodium metaperiodate deionized water dissolving
Person mixes, and lucifuge stirring 24h, adds ethylene glycol terminating reaction 15-20min equimolar with sodium metaperiodate, thoroughly at room temperature
Analysis removes unreacted material in 3 days, changes water 2-3 times daily, and dialysis is standby by dialyzate freeze-drying after terminating；It is again that gelatin is molten
In 40% acetum, it is stirring evenly and then adding into above-mentioned standby dialyzate and obtains mixed solution, electricity is added to after stirring
Spinning is carried out in frame, 12h in pH is 9 90% ethanol solution, drying for standby after taking-up is then immersed in；
C, the material for preparing step a and step b are according to mass ratio 20：1 mixing and stirring, then using the poly- of appropriate gauge
The packed mixed culture material of Acrylic plastic, side rim pressure, elasticity is suitable, and sterilizing, 126 in high-pressure sterilizing pot are placed in after tightening sack
DEG C 2-2.5h, takes out culture bag after natural cooling, nutrient solution is then configured under aseptic technique, and nutrient solution is sprayed onto into training
In nutriment, according still further to sterile working inoculation, inoculum concentration is advisable with strain covering charge level；
Described nutrient solution prescription is：Sodium selenite 4-5ppm, ammonium molybdate 6-8ppm, zinc sulfate 6-8ppm, calcium dihydrogen phosphate 4-
6ppm, ferrous sulfate 7-9ppm, KI 4-5ppm, add water to 1000mL；
D, the culturing room for postvaccinal culture bag being placed into dry, cleaning, being divulged information carry out half-light culture, and temperature control is in 18-
28 DEG C, relative air humidity is 65-75%, and a culture bag was turned over every 7-10 days, and mushroom house is produced after mycelia purseful long；
E, selection water quality it is good, quality is loose, the soil without miscellaneous worm's ovum, add 2-3% volcanic rock, the attapulgite and 5-8% of 3-5%
Deer natural pond soil mixing and stirring, then add 0.1% urea, 0.1% phosphate fertilizer, 1.5% lime and 0.1% carbendazim,
It is mixed into Nutrition Soil, and overlay film boring cover 2-3 days, the humidity for keeping Nutrition Soil is 45-55%, then by the bacterium in step d
Bag carries out vallum earthing, and the condition needed when then according to edible mushroom controls suitable humiture and ventilation condition.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107509530A (en) * | 2017-09-19 | 2017-12-26 | 迁西县林中宝生物科技有限公司 | A kind of big bacteria stick method that earthing fixed point fruiting does not cultivate chestnut mushroom |
| CN107950288A (en) * | 2017-11-20 | 2018-04-24 | 山东省农业科学院农业资源与环境研究所 | A kind of planting technique of straw mushroom |
| CN108812054A (en) * | 2018-05-30 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A method of cultivation Hericium erinaceus |
| CN110214626A (en) * | 2019-07-16 | 2019-09-10 | 灌南县人民政府蔬菜办公室 | A kind of straw mushroom cultural method |
| CN110476704A (en) * | 2019-09-25 | 2019-11-22 | 贵州山环菌草科技有限公司 | A kind of Volvaria volvacea cultivation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN104770222A (en) * | 2015-05-06 | 2015-07-15 | 青岛华盛绿能农业科技有限公司 | Method for inoculating stock spawn of edible fungi |
| CN105532257A (en) * | 2015-12-07 | 2016-05-04 | 武文礼 | Pleurotusostreatus and pleurotus eryngii high-yield cultivation method |
-
2016
- 2016-11-24 CN CN201611044375.3A patent/CN106718021A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN103155785B (en) * | 2011-12-09 | 2014-04-16 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN104770222A (en) * | 2015-05-06 | 2015-07-15 | 青岛华盛绿能农业科技有限公司 | Method for inoculating stock spawn of edible fungi |
| CN105532257A (en) * | 2015-12-07 | 2016-05-04 | 武文礼 | Pleurotusostreatus and pleurotus eryngii high-yield cultivation method |
Non-Patent Citations (1)
| Title |
|---|
| 覃宝山等: "板栗苞壳栽培杏鲍菇的试验", 《河地学院学报》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107509530A (en) * | 2017-09-19 | 2017-12-26 | 迁西县林中宝生物科技有限公司 | A kind of big bacteria stick method that earthing fixed point fruiting does not cultivate chestnut mushroom |
| CN107950288A (en) * | 2017-11-20 | 2018-04-24 | 山东省农业科学院农业资源与环境研究所 | A kind of planting technique of straw mushroom |
| CN107950288B (en) * | 2017-11-20 | 2020-08-21 | 山东省农业科学院农业资源与环境研究所 | Straw mushroom cultivation process |
| CN108812054A (en) * | 2018-05-30 | 2018-11-16 | 江苏圣福来生态农业有限公司 | A method of cultivation Hericium erinaceus |
| CN108812054B (en) * | 2018-05-30 | 2020-06-09 | 江苏圣福来生态农业有限公司 | Method for cultivating hericium erinaceus |
| CN110214626A (en) * | 2019-07-16 | 2019-09-10 | 灌南县人民政府蔬菜办公室 | A kind of straw mushroom cultural method |
| CN110214626B (en) * | 2019-07-16 | 2021-08-10 | 灌南县人民政府蔬菜办公室 | Straw mushroom culture method |
| CN110476704A (en) * | 2019-09-25 | 2019-11-22 | 贵州山环菌草科技有限公司 | A kind of Volvaria volvacea cultivation method |
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