CN106399123A - Quick spawn production method for edible fungi - Google Patents
Quick spawn production method for edible fungi Download PDFInfo
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- CN106399123A CN106399123A CN201610765308.4A CN201610765308A CN106399123A CN 106399123 A CN106399123 A CN 106399123A CN 201610765308 A CN201610765308 A CN 201610765308A CN 106399123 A CN106399123 A CN 106399123A
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Abstract
The invention discloses a quick spawn production method for edible fungi, and belongs to the technical field of edible fungi spawn production. The method comprises the steps of adding mother spawn of edible fungi to aseptic water according to a weight ratio of 1 to (10-20), performing homogenization, then performing uniform inoculation to a PDA culture medium or a full potato flour culture medium, and performing culture until the mother spawn grows all over on the culture medium; scraping and fetching the mother spawn on the culture medium, adding the mother spawn to a liquid culture medium for detection, and after culture for 2 days, observing whether the liquid culture medium is turbid and smelly or not; and inoculating a clear and no-smell mother spawn fungus liquid to a spawn culture medium, performing uniform mixing, and performing culture until the spawn grows all over on a cultural bag. The invention further discloses a method for detecting the purity of the mother spawn of the edible fungi. According to the spawn production method, the mother spawn is subjected to the purity detection step, so that the risk of spawn source pollution is avoided; moreover, the method is high in spawn production speed and high in fungus age consistency; and in addition, the spawn production method requires simple equipment and is low in production cost and suitable for spawn production by small and medium-sized enterprises and persons.
Description
Technical field
The invention belongs to edible fungus species production technical field is and in particular to a kind of method of edible fungi quick seed production.
Background technology
In Edible Fungi, strain quality quality directly determines the success or failure of production, and therefore, it is edible fungi that strain produces
The most key first step in production process.The edible fungus species using on producing at present mainly have 2 kinds, and a kind of is traditional agriculture
The used solid spawn of industry formula cultivation.Foundation《Edible fungus species management method》(Ministry of Agriculture promulgated on June 1st, 2006),
Edible fungus species production implementation parent species, original seed, the three-level of cultigen breed program.According to《Edible fungus species generic specifications》
(industry standard NY/T 1742-2009), it is Lentinus Edodess 14～16 days, Pleurotus ostreatus 7～9 that parent species cover with maximum duration needed for 90mm flat board
My god, Auricularia 14～16 days, Flammulina velutiper (Fr.) Sing 10～14 days, Agaricus bisporuss 28～32 days, Auricularia polytricha (Mout) Sacc. 14～16 days；Original seed covers with bottle
It is Lentinus Edodess 50 days, Pleurotus ostreatus 30 days, Auricularia 45 days, Flammulina velutiper (Fr.) Sing 35 days, Agaricus bisporuss 45 days, Auricularia polytricha (Mout) Sacc. 35 days for a long time；Cultivation
Plant and cover with maximum duration needed for bottle respectively Lentinus Edodess 45 days, Pleurotus ostreatus 25 days, Auricularia 40 days, Flammulina velutiper (Fr.) Sing 30 days, Agaricus bisporuss 40
My god, Auricularia polytricha (Mout) Sacc. 30 days.Therefore, this 6 class edible mashroom cultivating required time be about Lentinus Edodess 110 days, Pleurotus ostreatus 62 days, Auricularia 99 days,
Flammulina velutiper (Fr.) Sing 75 days, Agaricus bisporuss 113 days, Auricularia polytricha (Mout) Sacc. 79 days.It follows that traditional edible fungi agricultural formula cultivation solid used
Strain exist cycle length, production link is many, complex operation, growth efficiency are low, high cost, the shortcomings of easily cause strain pollution,
And the production accident that strain pollution (particularly polluted parent) causes, it is frequently experienced in (strain after large-scale inoculation cultivating bag
Pollution finds delayed), the loss causing is very big.Another kind is that edible fungi factory formula produces liquid spawn used.Liquid spawn
It is typically with biological culture (fermentation) equipment, by way of Submerged liquid culturation (fermentation), produce edible fungus species, its tool
Have that the production of hybrid seeds is fast, fruiting is neat, change of tide is fast, energetic, purity is high, anti-pollution, shorten cultivation period and low cost and other advantages, be suitable to into
Row standardization, batch production and anniversary metaplasia are produced, and are the development trends of edible fungi.But liquid spawn preparation exists needs higher throwing
The hardware device of money, builds between aseptic inoculation, technical requirements are high, the shortcomings of need to engage professional and technical personnel；For little producer
Or certainly do the peasant household of strain, make strain difficulty using fermentation tank very big.Therefore there is no people during agriculture formula production edible fungi
Using liquid spawn.
From the foregoing, solid spawn urgent need to resolve long the production cycle used and strain pollution during agriculture formula is cultivated at present
The problems such as find delayed.
Content of the invention
For traditional edible fungus solid spawn produce present in long the production cycle, growth efficiency be low, strain pollution is sent out
Existing delayed the shortcomings of, present invention aim at provide a kind of edible fungi quick seed production method.
Another object of the present invention is to provide a kind of propagation method of edible fungi parent species.
The present invention the 3rd purpose is to provide the detection method of edible fungi parent species purity.
The present invention the 4th purpose is to provide a kind of detection fluid medium.
The present invention the 5th purpose is to provide use in edible fungus species purity detecting for the above-mentioned detection fluid medium
On the way.
In order to reach as above purpose, the present invention adopts the following technical scheme that：
The invention provides a kind of edible fungi quick seed production method, comprise the steps:
(1), parent species breeding：By edible fungi parent species by weight 1：10～20 ratio is added in sterilized water, homogenate, obtains
Homogenate bacterium solution；Homogenate bacterium solution is evenly distributed in PDA plate culture medium or potato full-powder culture medium；In thermophilic, lucifuge bar
3-7d is cultivated, till surface covers with part；
(2), purity detecting：The parent species covering with PDA plate culture medium or potato full-powder culture medium in scraping step (1)
Mycelium, according to weight between parent species mycelium and detection fluid medium than for 1:5～10 ratio, by parent species mycelium
It is placed in detection fluid medium, homogenate；Then quiescent culture 2d under the conditions of 25～28 DEG C, lucifuge；Observe detection liquid
Whether body culture medium is muddy, if having abnormal flavour；Select and remain no not muddy, the parent species bacterium solution of free from extraneous odour；
(3), parent species dilution, inoculates Cultivar culture medium：Step (2) gained is no muddy, the parent species bacterium solution of free from extraneous odour,
Subpackage, to aseptic triangular flask, compares 1 according to weight：10～15 ratio dilutes parent species bacterium solution with sterilized water；According still further to weight ratio
Parent species bacterium solution after diluting is inoculated in Cultivar culture medium ratio for 5～8%；During inoculation, first take off cultigen culture
Strain lid outside base, transfers to the sticking plaster at Cultivar culture medium center, exposes medium pore, parent species bacterium solution is poured in medium pore,
Cover strain lid, keep flat bacterium bag, allow bacterium solution to penetrate on culture basal wall, when putting, by bacterium bag turn-over, make the one of bacterium solution to face
On, bacterium solution is permeated to culture medium another side, thus reaching the effect that culture medium is mixed with bacterium solution；Enter under the conditions of thermophilic, lucifuge
Row culture, till mycelia covers with culture medium, obtains final product the cultigen of this edible fungi.
Thermophilic described in above-mentioned producing method for seed step (1) is 23～29 DEG C, different according to edible fungi, selects accordingly
Thermophilic degree, actual temp according to《Edible fungus species generic specifications》The standard of (industry standard NY/T 1742-2009) table 3
Carry out.
PDA plate culture medium described in above-mentioned preparation method step (1) is conventionally prepared.
The constituent of potato full-powder culture medium described in above-mentioned preparation method step (1) and its ratio are：Ma Ling
Potato full powder 10～20g, glucose 2～10g, agar powder 12～20g, water 1000ml.
The preparation method of described potato full-powder culture medium is：According to weight ratio weigh potato full-powder, glucose and
Agar powder, mix homogeneously, it is subsequently adding in container (as triangular flask), is proportionally added into water, shakes up, seal up sealed membrane or Corii Bovis seu Bubali
Paper, 121 DEG C of sterilizing 30min, standby.Wherein potato full-powder, glucose and agar powder are all commercially available obtains.
The constituent of detection fluid medium described in above-mentioned producing method for seed step (2) and its ratio are：Fructus Vitis viniferae
Sugared 20g, yeast powder 2g, water 1000ml.
The preparation method of described detection fluid medium is：In proportion glucose, yeast powder are added to the water, mix
Close uniformly, in 121 DEG C of 30min that sterilize.
The raw material of the Cultivar culture medium described in above-mentioned producing method for seed step (3) is wheat grain, wood flour, cotton seed hullss or Semen Maydiss
Core etc.；Select the Cultivar culture medium of different raw materials making by edible fungi species.
Thermophilic described in above-mentioned producing method for seed step (3) is 20～31 DEG C；Different edible fungi selects different cultures
Temperature.Actual temp according to《Edible fungus species generic specifications》The regulation of table 6 in (industry standard NY/T1742-2009)
Temperature is cultivated.
Present invention also offers a kind of propagation method of edible fungi parent species, including by edible fungi parent species by weight 1：10～
20 ratio is added in sterilized water, homogenate, obtains homogenate bacterium solution；Homogenate bacterium solution is evenly distributed on PDA plate culture medium or horse
In bell potato full powder culture medium；Cultivate 3-7d under the conditions of thermophilic, lucifuge, till surface covers with.
Thermophilic described in above-mentioned propagation method is 23～29 DEG C, according to the corresponding optimum temperature of edible fungi different choice,
Actual temp can be found in《Edible fungus species generic specifications》The standard of (industry standard NY/T1742-2009) table 3 is carried out.
Present invention also offers the detection method of edible fungi parent species purity, comprise the steps：
(1) make detection fluid medium according to the ratio containing glucose 20g and yeast powder 2g in every 1000ml water；
(2) scraping covers with the edible fungi parent species in PDA plate culture medium or potato full-powder culture medium, by weight 1:5
～10 ratio, is added in detection fluid medium prepared by step (1), homogenate；Again detection fluid medium is existed
25～28 DEG C, quiescent culture 2d under the conditions of lucifuge；
(3) whether muddy observe culture medium, if having abnormal flavour；No muddy, as pollution-free, the high food of purity of free from extraneous odour
Use starter kind.
Present invention also offers a kind of detection fluid medium, its constituent and its ratio are：Glucose 20g, ferment
Female powder 2g, water 1000ml.
Above-mentioned detection fluid medium is in the application in edible fungus species purity detecting.
The present invention has the advantage that and beneficial effect：(1), in producing method for seed of the present invention parent species through purity detecting step,
Avoid the risk of strain source pollution.(2), the inventive method strain manufacturing speed is fast, and cell age concordance is high.(a), the present invention
In method parent species take strain media surface be coated with mode make, bacterium after sprouting compared to conventional central vaccination
Silk is stretched to around culture medium, and this method makes media surface all be distributed vaccination, covers with 1 day about after mycelium germination flat
Plate, the time of covering with only has about the half required for conventional method.(b), take parent species direct inoculation Cultivar culture medium, save
Original seed making link, the time required for strain that makes greatly shortens, and the time as required for Pleurotus ostreatus by conventional 60 days about, contracts
It is as short as 13-16 days；Time required for Lentinus Edodess, by conventional 110 days about, foreshortens to 24-26 days.During the production of hybrid seeds of (c) routine, mycelia
Grow to bottom from upper surface, the required time is long, surface is mutually far short of what is expected with the cell age of bottom surface, such as Pleurotus ostreatus differ 20 days about,
Lentinus Edodess differ 40 days about.And the present invention adopts multiple spot to sprout, bacterium solution contacts to the place of compost, is all germination point, so
Cell age concordance is very high, and Pleurotus ostreatus differ 3 days about, and Lentinus Edodess differ 7 days about.Cell age concordance is high, then send out bacterium speed on cultivating bag
Spend roughly the same, suitable management of producing mushroom.(3), producing method for seed equipment needed thereby of the present invention is simple, low production cost.Traditional production of hybrid seeds
Mode, due to there being original seed making link, incubation time is very long, and culturing room's turnover rate is low, and power consumption is high.And carry out liquid using fermentation tank
Body is cultivated, since it is desired that buying fermentation tank, building between aseptic inoculation, engaging and understand technical staff of fermentation etc., thus high cost.And
It is only necessary to superclean bench, triangular flask, homogenizer just can reach the effect that fermentation tank makes liquid spawn, flexible machine to the present invention
Dynamic.The present invention is suitable for medium-sized and small enterprises, personal system strain.
Brief description：
Fig. 1. it is not affected by the bacterium solution photo of germ contamination during parent species purity detecting.
Fig. 2. it is subject to the bacterium solution photo of germ contamination during parent species purity detecting.
Fig. 3. Pleurotus ostreatus cultivate 6 days mycelia photos after using parent species bacterium solution inoculation of the present invention.
Fig. 4. Pleurotus ostreatus cultivate 6 days mycelia photos after using solid spawn inoculation.
Fig. 5. Flammulina velutiper (Fr.) Sing cultivates 20 days mycelia photos after using parent species bacterium solution inoculation of the present invention.
Fig. 6. Flammulina velutiper (Fr.) Sing cultivates 20 days mycelia photos after using solid spawn inoculation.
Fig. 7. Lentinus Edodess cultivate 25 days mycelia photos after using parent species bacterium solution inoculation of the present invention.
Fig. 8. Lentinus Edodess cultivate 25 days mycelia photos after using solid spawn inoculation.
Fig. 9. Auricularia cultivates 25 days mycelia photos after using parent species bacterium solution inoculation of the present invention.
Figure 10. Auricularia cultivates 25 days mycelia photos after using solid spawn inoculation.
Figure 11. Pleurotus nebrodensis cultivate 18 days mycelia photos after using parent species bacterium solution inoculation of the present invention.
Figure 12. Pleurotus nebrodensis cultivate 18 days mycelia photos after using solid spawn inoculation.
Figure 13. Pleurotus eryngii cultivates 18 days mycelia photos after using parent species bacterium solution inoculation of the present invention.
Figure 14. Pleurotus eryngii cultivates 18 days mycelia photos after using solid spawn inoculation.
Specific embodiment：
The invention will be further described by the following examples, but the present invention is not limited in any way.
Embodiment 1 flat mushroom strain makes contrast test
Carry out as follows：
(1) Fast-propagation of parent species aseptically, by weight 1：Parent species are added in sterilized water 15 ratios, even
Slurry, obtains homogenate bacterium solution；It is uniformly distributed in PDA plate media surface with aseptic watering can or spreader by being homogenized bacterium solution, seal up training
Foster ware edge, cultivates 3～4 days, mycelia covers with flat board, as parent species under the conditions of 25 DEG C, lucifuge.
(2) detection of parent species purity load in 250ml triangular flask detection fluid medium (its constituent be 20g
Glucose, 2g yeast powder, water 1000ml) 100ml, at 121 DEG C, 30min sterilizing, is cooled to room temperature stand-by.Will be long in step (1)
Full PDA plate culture medium, 1 flat board corresponds to 1 triangular flask, on scraping flat board all culture medium to triangular flask, aseptic bar
Under part, homogenate；Quiescent culture 2d under the conditions of 25 DEG C, lucifuge, obtains parent species bacterium solution；Whether muddy observe culture medium, if having different
Taste.Culture medium clarifies (see Fig. 1), free from extraneous odour, illustrates that parent species are pure, can carry out next step expanding propagation；Otherwise, culture medium is mixed
Turbid (see Fig. 2), then polluted parent is it is impossible to use.
(3) parent species dilution, inoculates Cultivar culture medium：By the parent species bacterium solution of clarification, free from extraneous odour, according to 1：10 ratio adds
Enter sterilized water dilution；By sterilized wheat grain culture medium, (constituent is 98% wheat grain, and 2% Gypsum Fibrosum is shown in NY/T 528-
2010 edible fungus species production technology regulation Appendix B .5.Wheat grain clear water soaks 24-36h, picks up and drains, and is proportionally added into stone
Cream powder, after mixing, pack, 126 DEG C of sterilizing 2h, it is cooled to room temperature), the inoculum concentration for 5% by weight percentage, accesses dilution
Parent species bacterium solution, shake up.Put into culturing room, cultivate 8-10 days under the conditions of 25 DEG C, lucifuge, till covering with culture bag.
Make conventional solid spawn with traditional method, (formula is identical, directly from strain mouth for inoculation wheat grain respectively simultaneously
Pour fluid medium into or access solid medium to wheat grain surface), cultivate under the same terms.
6 days (see Fig. 3) is cultivated, mycelia is close to be covered with wheat after the parent species bacterium solution inoculation that result is made using the inventive method
Grain, culture is all covered with for 9 days about；And using traditional solid spawn after inoculating, to cultivate 6 days (see Fig. 4), mycelia has just sprouted few
Permitted, final culture is covered with for 23 days.
It can be seen from the results above that compared to using traditional solid spawn, the Pleurotus ostreatus being made using the inventive method
Parent species bacterium solution inoculates wheat grain, and the production of hybrid seeds time shortens 60%.The inventive method edible mashroom cultivating cycle is short is described, production of hybrid seeds speed is fast,
And avoid the problem of polluted parent.
Embodiment 2 Flammulina velutiper (Fr.) Sing, Lentinus Edodess and Auricularia strain make contrast test
Carry out as follows：
(1) Fast-propagation of parent species aseptically, by weight 1：10 ratio is by Flammulina velutiper (Fr.) Sing, Lentinus Edodess or Auricularia
Parent species are added in sterilized water, homogenate, obtain homogenate bacterium solution；Using aseptic watering can or spreader, homogenate bacterium solution is uniformly distributed in
PDA culture medium surface, seals up culture dish edge, cultivates 6～7 days in 25 DEG C of lucifuges, mycelia covers with flat board, as parent species.
(2) detection method of parent species purity is with embodiment 1 step (2).
(3) parent species dilution, inoculation Cultivar culture medium, by the clarification of gained in step (2), the parent species bacterium solution of free from extraneous odour, is pressed
Compare 1 according to weight：10 are diluted with sterilized water.Sterilized sawdust medium (its constituent is 78% wood flour, 20% wheat bran,
1% sucrose, 1% Gypsum Fibrosum, are shown in NY/T 528-2010 edible fungus species production technology regulation Appendix B .1.1.Weigh wood in proportion
Bits, wheat bran, Gypsum Fibrosum, mix, sucrose are added to the water, by compost：The weight of water is than for 2：3, add water in compost, mix
Even, so that water is fully absorbed, pack, central authorities' insertion hollow plastic stick, non-cotton cover seals, 126 DEG C of sterilizing 2h, is cooled to room
Temperature) by weight percentage be 5% ratio by dilute after parent species bacterium solution be inoculated on sawdust medium, by parent species bacterium solution and training
Foster base mixes.Put into culturing room, cultivate 22～28 days under the conditions of 25 DEG C, lucifuge, cover with strain bag.
Use conventional solid spawn, (formula is identical, transfers to the hollow plastics in central authorities for inoculation sawdust medium respectively simultaneously
Rod, directly pours fluid medium into central authorities from strain mouth；Or access solid medium to sawdust medium surface), identical
Under the conditions of cultivate.
20 days (see Fig. 5) is cultivated, mycelia is covered with after the parent species bacterium solution inoculation that result Flammulina velutiper (Fr.) Sing is made using the inventive method
Sawdust medium；And after using solid spawn inoculation, cultivate 20 days (see Fig. 6), mycelia covers with about 4/5.
25 days (see Fig. 7) is cultivated, mycelia is close to cover with wood after the parent species bacterium solution inoculation that Lentinus Edodess are made using the inventive method
Bits culture medium；And using traditional solid spawn after inoculating, to cultivate 25 days (see Fig. 8), mycelia covers with about 4/5.
25 days (see Fig. 9) is cultivated, mycelia is close to cover with after the parent species bacterium solution inoculation that Auricularia is made using the inventive method
Sawdust medium；And using traditional solid spawn after inoculating, to cultivate 25 days (see Figure 10), mycelia covers with about 1/2.
Can be seen that compared to using traditional solid spawn from above-mentioned comparative test result, using the inventive method system
The Flammulina velutiper (Fr.) Sing of work, Lentinus Edodess, the parent species bacterium solution inoculation sawdust medium of Auricularia, the production of hybrid seeds time shortens more than 10 days.This is described
Bright producing method for seed cycle is short, speed are fast, and are avoided that polluted parent.
Embodiment 3 Pleurotus nebrodensis, pleurotus eryngii quel strains make contrast test
Carry out as follows：
(1) Fast-propagation of parent species aseptically, by weight 1：10 ratio is by Pleurotus nebrodensis or Pleurotus eryngii parent species
It is added in sterilized water, homogenate, obtain homogenate bacterium solution；Using aseptic watering can or spreader, homogenate bacterium solution is uniformly distributed in PDA training
Foster primary surface, seals up culture dish edge, cultivates 4～5 days, mycelia covers with flat board, as parent species under the conditions of 25 DEG C, lucifuge.
(2) detection of parent species purity is with embodiment 1 step (2).
(3) parent species dilution, inoculation Cultivar culture medium, by the clarification of gained in step (2), the parent species bacterium solution of free from extraneous odour, is pressed
Compare 1 according to weight：10 ratio adds sterilized water dilution.In sterilized cotton seed hullss culture medium, (its constituent is 99% Semen Gossypii
Shell, 1% Calx, see NY/T 528-2010 edible fungus species production technology regulation Appendix B .3.1.Weigh cotton seed hullss, stone in proportion
Ash, by compost：The weight of water is than for 2：3, add water in compost, mix, so that water is fully absorbed, pack, central authorities insert
Entering hollow plastic stick, non-cotton cover seals, 126 DEG C of sterilizing 2h, be cooled to room temperature) by weight percentage 5% ratio will dilute
Parent species bacterium solution be inoculated in cotton seed hullss culture medium, shake up；Put into culturing room, cultivate 20～23 days under the conditions of 25 DEG C, lucifuge,
Cover with strain bag.
Use conventional solid spawn, (formula is identical, transfers to the hollow plastics in central authorities for inoculation cotton seed hullss culture medium respectively simultaneously
Rod, directly pours fluid medium into central authorities from strain mouth；Or access solid medium to kapok seed shell media surface),
Cultivate under the same terms.
18 days (see Figure 11) is cultivated, mycelia covers with Semen Gossypii after the parent species bacterium solution that result Pleurotus nebrodensis are made using the inventive method
About the 2/3 of shell culture medium；And after using traditional solid spawn, cultivate 16 days (see Figure 12), mycelia covers with about 1/3.
18 days (see Figure 13) is cultivated, mycelia covers with Semen Gossypii after the parent species bacterium solution inoculation that Pleurotus eryngii is made using the inventive method
About the 4/5 of shell culture medium；And after using solid spawn inoculation, cultivate 18 days (see Figure 14), mycelia covers with about 1/3.
Can be seen that compared to using traditional solid spawn, the Pleurotus nebrodensis making of the inventive method, Fructus Pruni from upper result
The parent species bacterium solution inoculation cotton seed hullss culture medium of abalone mushroom, the production of hybrid seeds time shortens 5-10 days.The inventive method production of hybrid seeds cycle is short is described, system
Plant speed fast, and avoid the problem of polluted parent.
Additionally, the above results also illustrate simple using the inventive method producing method for seed, required equipment simply, make
Low cost.
Claims (9)
1. a kind of edible fungi quick seed production method is it is characterised in that comprise the steps:
(1), by edible fungi parent species by weight 1：10～20 ratio is added in sterilized water, homogenate, obtains homogenate bacterium solution；Will be even
Slurry bacterium solution is evenly distributed in PDA plate culture medium or potato full-powder culture medium；Cultivate 3-7d under the conditions of thermophilic, lucifuge,
Till surface covers with；
(2), the parent species mycelium covering with PDA plate culture medium or potato full-powder culture medium in scraping step (1), according to mother
Between kind of mycelium and detection fluid medium, weight ratio is for 1:5～10 ratio, parent species mycelium is placed in detection liquid
In body culture medium, homogenate；Then quiescent culture 2d under the conditions of 25～28 DEG C, lucifuge；Whether observe detection fluid medium
Muddy, if to have abnormal flavour；Select and remain no not muddy, the parent species bacterium solution of free from extraneous odour；
(3), that step (2) gained is no muddy, the parent species bacterium solution of free from extraneous odour, in subpackage to aseptic triangular flask, according to weight ratio
1：10～15 ratio dilutes parent species bacterium solution with sterilized water；According still further to weight than the ratio for 5～8% by dilute after parent species bacterium
Liquid is inoculated in Cultivar culture medium；During inoculation, first take off the strain lid outside Cultivar culture medium, transfer in Cultivar culture medium
The sticking plaster of the heart, exposes medium pore, and parent species bacterium solution is poured in medium pore, covers strain lid, keeps flat bacterium bag, allows bacterium solution to penetrate into
On culture basal wall, when putting, by bacterium bag turn-over, the one of bacterium solution is made to face up, bacterium solution is permeated to culture medium another side, thus reaching
The effect mixing with bacterium solution to culture medium；Cultivated under the conditions of thermophilic, lucifuge, till mycelia covers with culture medium, obtained final product this
The cultigen of edible fungi.
2. producing method for seed according to claim 1 is it is characterised in that the thermophilic described in its step (1) is 23～29 DEG C.
3. producing method for seed according to claim 1 is it is characterised in that the potato full-powder culture medium described in its step (1)
Constituent and its ratio be：Potato full-powder 10～20g, glucose 2～10g, agar powder 12～20g, water 1000ml.
4. producing method for seed according to claim 1 is it is characterised in that the detection fluid medium described in its step (2)
Constituent and its ratio be：Glucose 20g, yeast powder 2g, water 1000ml.
5. producing method for seed according to claim 1 is it is characterised in that the thermophilic described in its step (3) is 20～31 DEG C.
6. a kind of propagation method of edible fungi parent species is it is characterised in that include edible fungi parent species by weight 1：10～20 ratio
Example is added in sterilized water, homogenate, obtains homogenate bacterium solution；Homogenate bacterium solution is evenly distributed on PDA plate culture medium or Rhizoma Solani tuber osi is complete
In powder culture medium；Cultivate 3-7d under the conditions of thermophilic, lucifuge, till surface covers with.
7. a kind of detection method of edible fungi parent species purity is it is characterised in that comprise the steps：
(1) make detection fluid medium according to the ratio containing glucose 20g and yeast powder 2g in every 1000ml water；
(2) scraping covers with the edible fungi parent species in PDA plate culture medium or potato full-powder culture medium, by weight 1:5～10
Ratio, be added in detection fluid medium prepared by step (1), homogenate；Again by detection fluid medium 25～
28 DEG C, quiescent culture 2d under the conditions of lucifuge；
(3) whether muddy observe culture medium, if having abnormal flavour；No muddy, free from extraneous odour as pollution-free, edible fungi that purity is high
Parent species.
8. a kind of detection fluid medium is it is characterised in that its constituent and its ratio are：Glucose 20g, yeast powder 2g,
Water 1000ml.
9. the detection fluid medium described in claim 8 is in the application in edible fungus species purity detecting.
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| CN104718996A (en) * | 2015-04-13 | 2015-06-24 | 青岛华盛绿能农业科技有限公司 | Method for manufacturing edible mushroom solid liquefied strains |
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