CN110301291A - A kind of breeding method of hickory chick strain - Google Patents
A kind of breeding method of hickory chick strain Download PDFInfo
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- CN110301291A CN110301291A CN201810229511.9A CN201810229511A CN110301291A CN 110301291 A CN110301291 A CN 110301291A CN 201810229511 A CN201810229511 A CN 201810229511A CN 110301291 A CN110301291 A CN 110301291A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
Abstract
The invention belongs to breed of edible fungus technical fields, and in particular to a kind of breeding method of hickory chick strain, comprising: the production and culture of parent species, the production of original seed and culture, the production and culture of culture kind.The breeding method of hickory chick strain of the invention makes an adjustment each culture medium in the cultivating process of hickory chick strain, and the preparation method of each culture medium is also all made adjustment, it joined potassium dihydrogen phosphate, magnesium sulfate and corn flour in culture medium, so that parent species draw more nutriments from culture medium, avoids strain display form in the reproductive process in later period inconsistent with female parent and influence yield.
Description
Technical field
The invention belongs to breed of edible fungus technical fields, and in particular to a kind of breeding method of hickory chick strain.
Background technique
Hickory chick (Morchella), also known as sheep tripe dish, sheep tripe mushroom etc., mycology classification belong to Pezizale, Morchellaceae, sheep
Tripe Pseudomonas.Its by sheep tripe shape can pregnant capitulum cap and an infertile stem form, cap surface has the son of netted rib real
Layer, edge are connected with stem, and stem is cylindric, hollow, and smooth surface or fluted, form exactly likes sheep tripe and gains the name.Hickory chick
Contain C-3- Amino-L-proline, aminoisobutyric rich in 8 kinds of amino acid needed by human and vitamins, especially hickory chick
The rare amino acids such as acid and 2,4- diaminoisobutyric acid, thus there is its peculiar flavour, it is foremost precious edible in the world
One of bacterium.Its domestication's plantation is always that fungus scholar in home and abroad endeavours one of project of research and probe.
Hickory chick is generally grown in 2100~3400 meters of height above sea level of forest zone, because tighter to environment required by growth and development
Lattice, therefore realize that artificial growth has certain difficulty.Currently, the general cultural method of hickory chick mainly has: (1) plant formulation
Cultivation；(2) indoor de- bag cultivating；(3) outdoor de- bag cultivating.However, cultivating the mistake of hickory chick using above-mentioned general breeding method
Cheng Zhong can encounter homocellular hyphal cell performance difference, and mycelia growth but has different performances under identical conditions, especially
Be every inoculation it is primary all grown with previous mycelia it is different, no matter the length of mycelia, thickness, speed, color, growing way and last
Performance it is all different, even it is also in this way, taking same original that original used or First Year, which went out the old strain of mushroom,
Material, formula, operating method, same temperature and humidity and interior external condition, but show biggish gap, therefore, how to cultivate
The stable strain of performance, so that later product quality stabilization is particularly important.
Summary of the invention
Therefore, the technical problem to be solved in the present invention is that overcoming hickory chick strain stability is poor in the prior art to ask
Topic, to provide a kind of hickory chick method of breeding strain that performance is stable.
In order to solve the above technical problems, the present invention provides a kind of breeding methods of hickory chick strain, comprising:
(1) production and culture of parent species
A. mother culture media is made:
The raw material group of the mother culture media becomes, based on parts by weight: 120~180 parts of peeled potatoes, glucose 11
~18 parts, 12~16 parts of agar, 0.4~0.6 part of potassium dihydrogen phosphate, 0.3~0.5 part of magnesium sulfate, 3~7 parts of corn flour；
By the peeled potatoes stripping and slicing, adds boiling to boil 15~20 minutes, filtered after smashing to pieces, obtain filtrate；To above-mentioned filter
Glucose, agar, potassium dihydrogen phosphate, magnesium sulfate and the corn flour of above-mentioned parts by weight are added in liquid, it is completely molten to be heated to agar
Change, centrifugation takes supernatant, the high pressure sterilization 30min under 0.1MPa pressure, takes out cooling to get mother culture media；
B. the culture of parent species: removing stem for robust growth, a Morchella esculenta (L.) Pers sporophore big, that growing way is good in wild environment,
The alcohol surface sterilization that remaining cap volumetric concentration is 70%, and with aseptic water washing, after to be dried, cut 5mm × 5mm
2~3 pieces of bacterial context, access in the mother culture media, be placed in incubator and cultivate, until grow up to parent species；The incubator
Temperature is 16 DEG C~20 DEG C；
(2) production and culture of original seed
A. pedigree seed culture medium is made:
The raw material group of the pedigree seed culture medium becomes, based on parts by weight: 70~80 parts of apple sawdust, wheat 15~20
Part, 0.5~1.5 part of lime, 0.5~1.5 part of gypsum, 3~7 parts of soil；
The each component of above-mentioned parts by weight is mixed, is added water and stirred, the content for controlling moisture is 60~70%, is pressed in 0.2MPa
1.5~2h of high pressure sterilization under power takes out cooling to get pedigree seed culture medium；
B. the culture of original seed: in inoculating hood, the parent species are inoculated into the pedigree seed culture medium；The inoculating hood
Temperature is maintained at 25 DEG C~30 DEG C；
(3) production and culture of culture kind
A. the fluid nutrient medium of kind is cultivated in production:
The raw material group of the pedigree seed culture medium becomes, based on parts by weight, 6~10 parts of urea, 10~12 parts of bagasse, wheat
Grain 32~38 parts, 6~9 parts of glucose, 10~15 parts of yeast extract, 5~10 parts of corn pulp, 8~10 parts of calcium chloride, ironic citrate 3
~6 parts, 3~5 parts of calcium chloride, 1~5 part of gypsum；
The each component of above-mentioned parts by weight is mixed, filter residue obtains culture solution, and sterilize 10~20min under 0.15Pa pressure,
Cooling is taken out to get fluid nutrient medium is arrived；
B. in inoculating hood, the pedigree seed culture medium is added in the fluid nutrient medium, that is, obtains culture kind；It is described
The temperature of inoculating hood is maintained at 15 DEG C~20 DEG C.
Further, the breeding method of the hickory chick strain further includes the culture kind dislocation for obtaining step (3)
The step of being cultivated in culturing room.
Further, the temperature of the culturing room be 8 DEG C~18 DEG C, relative air humidity≤65%.
Technical solution of the present invention has the advantages that
(1) present invention makes an adjustment each culture medium in the cultivating process of hickory chick strain, and each culture
The preparation method of base is also all made adjustment, and joined potassium dihydrogen phosphate, magnesium sulfate and corn flour in culture medium, so that parent species are from training
It supports in base and draws more nutriments, effectively avoid strain display form and female parent in the reproductive process in later period inconsistent,
Influence yield.
(2) fluid nutrient medium is made in culture medium, and more multistability and the substance rich in nutrient is added, such as urea, wheat
Bran etc., it is not easy to be invaded by miscellaneous bacteria, therefore, be able to maintain the stability of strain, avoid strain in the reproductive process in later period
Display form and female parent are inconsistent, influence yield.
(3) it selects robust growth in wild environment, a hickory chick big, that growing way is good to make parent species, further improves
The stability of strain.
(4) high pressure sterilization is carried out to the culture medium of each step, has prevented the intrusion of bacterium etc., has improved the stability of strain；
The temperature for controlling inoculating hood and culturing room, provides advantage for the fast-growth of strain.
Specific embodiment
Technical solution of the present invention will be clearly and completely described below, it is clear that described embodiment is this hair
Bright a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, shall fall within the protection scope of the present invention.In addition, below
Technical characteristic involved in described different embodiments of the present invention as long as they do not conflict with each other can be mutual
In conjunction with.
Embodiment 1
The present embodiment provides a kind of breeding methods of hickory chick strain, the specific steps are as follows:
(1) production and culture of parent species
A. it makes mother culture media: weighing peeled potatoes 160g, glucose 16g, agar 14g, potassium dihydrogen phosphate respectively
Peeling potatoes, washing, stripping and slicing are added boiling to boil 18 minutes, filter, obtain after smashing to pieces by 0.5g, magnesium sulfate 0.4g, corn flour 5g
To filtrate；Glucose, agar, potassium dihydrogen phosphate, magnesium sulfate and the corn flour of above-mentioned quality are added into above-mentioned filtrate, is heated to
Agar dissolves completely, and centrifugation takes supernatant, the high pressure sterilization 30min under 0.1MPa pressure, takes out cooling to get Mother culture
Base；
B. the culture of parent species: removing stem for robust growth, a Morchella esculenta (L.) Pers sporophore big, that growing way is good in wild environment,
Remaining cap volumetric concentration is 70% alcohol surface sterilization, and uses aseptic water washing.After to be dried, 5mm × 5mm is cut
2 pieces of bacterial context, access in the mother culture media, being placed in temperature is to cultivate in 18 DEG C of incubator, until growing up to parent species.
(2) production and culture of original seed
A. it makes pedigree seed culture medium: weighing respectively: apple sawdust 75g, wheat 18g, lime 1g, gypsum 1g, soil 5g, it will
The each component of above-mentioned quality mixes, and adds water and stirs, and the content for controlling moisture is 65%, the high pressure sterilization under 0.2MPa pressure
1.8h takes out cooling to get pedigree seed culture medium；
B. the culture of original seed: in the inoculating hood that temperature is 28 DEG C, parent species are inoculated into pedigree seed culture medium.
(3) production and culture of culture kind
A. the fluid nutrient medium of kind is cultivated in production: being weighed respectively: urea 8g, bagasse 11g, wheat 35g, glucose
7.5g, yeast extract 12.5g, corn pulp 7.5g, calcium chloride 9g, ironic citrate 4.5g, calcium chloride 4g, gypsum 3g, by above-mentioned component
Mixing, filter residue obtain culture solution, and sterilize 15min under 0.15Pa pressure, take out cooling to get fluid nutrient medium is arrived；
B. in the inoculating hood that temperature is 18 DEG C, original seed is inoculated into the fluid nutrient medium, that is, obtains culture kind, it will
It is cultivated in culture kind dislocation culturing room, the temperature of culturing room is 12 DEG C, relative air humidity≤65%.
Embodiment 2
The present embodiment provides a kind of breeding methods of hickory chick strain, the specific steps are as follows:
(1) production and culture of parent species
A. it makes mother culture media: weighing peeled potatoes 120g, glucose 11g, agar 12g, potassium dihydrogen phosphate respectively
Peeling potatoes, washing, stripping and slicing are added boiling to boil 15 minutes, filter, obtain after smashing to pieces by 0.4g, magnesium sulfate 0.3g, corn flour 3g
To filtrate；Glucose, agar, potassium dihydrogen phosphate, magnesium sulfate and the corn flour of above-mentioned quality are added into above-mentioned filtrate, is heated to
Agar dissolves completely, and centrifugation takes supernatant, the high pressure sterilization 30min under 0.1MPa pressure, takes out cooling to get Mother culture
Base；
B. the culture of parent species: removing stem for robust growth, a Morchella esculenta (L.) Pers sporophore big, that growing way is good in wild environment,
Remaining cap volumetric concentration is 70% alcohol surface sterilization, and uses aseptic water washing.After to be dried, 5mm × 5mm is cut
3 pieces of bacterial context, access in the mother culture media, being placed in temperature is to cultivate in 16 DEG C of incubator, until growing up to parent species.
(2) production and culture of original seed
A. it makes pedigree seed culture medium: weighing respectively: apple sawdust 70g, wheat 15g, lime 0.5g, gypsum 0.5g, soil
The each component of above-mentioned quality is mixed, is added water and stirred by 3g, and the content for controlling moisture is 70%, and high pressure is gone out under 0.2MPa pressure
Bacterium 1.5h takes out cooling to get pedigree seed culture medium；
B. the culture of original seed: in the inoculating hood that temperature is 25 DEG C, parent species are inoculated into pedigree seed culture medium.
(3) production and culture of culture kind
A. production culture kind fluid nutrient medium: weigh respectively: urea 6g, bagasse 10g, wheat 32g, glucose 6g,
Yeast extract 10g, corn pulp 5g, calcium chloride 8g, ironic citrate 3g, calcium chloride 3g, gypsum 1g mix above-mentioned component, and filter residue obtains
To culture solution, sterilize 10min under 0.15Pa pressure, takes out cooling to get fluid nutrient medium is arrived；
B. in the inoculating hood that temperature is 15 DEG C, pedigree seed culture medium is added in the fluid nutrient medium, that is, is trained
It feeding kind, will be cultivated in culture kind dislocation culturing room, the temperature of culturing room is 18 DEG C, relative air humidity≤62%.
Embodiment 3
The present embodiment provides a kind of breeding methods of hickory chick strain, the specific steps are as follows:
(1) production and culture of parent species
A. it makes mother culture media: weighing peeled potatoes 180g, glucose 18g, agar 16g, potassium dihydrogen phosphate respectively
Peeling potatoes, washing, stripping and slicing are added boiling to boil 20 minutes, filter, obtain after smashing to pieces by 0.6g, magnesium sulfate 0.5g, corn flour 7g
To filtrate；Glucose, agar, potassium dihydrogen phosphate, magnesium sulfate and corn flour are added into above-mentioned filtrate, it is completely molten to be heated to agar
Change, centrifugation takes supernatant, the high pressure sterilization 30min under 0.1MPa pressure, takes out cooling to get mother culture media；
B. the culture of parent species: removing stem for robust growth, a Morchella esculenta (L.) Pers sporophore big, that growing way is good in wild environment,
The alcohol surface sterilization that remaining cap volumetric concentration is 70%, and with aseptic water washing, after to be dried, cut 5mm × 5mm
3 pieces of bacterial context, access in the mother culture media, being placed in temperature is to cultivate in 18 DEG C of incubator, until growing up to parent species.
(2) production and culture of original seed
A. it makes pedigree seed culture medium: weighing respectively: apple sawdust 80g, wheat 20g, lime 1.5g, gypsum 1.5g, soil
Above-mentioned each component is mixed, is added water and stirred by 7g, and the content for controlling moisture is 60%, and high pressure sterilization 2h, takes under 0.2MPa pressure
It cools down out to get pedigree seed culture medium；
B. the culture of original seed: in the inoculating hood that temperature is 30 DEG C, parent species are inoculated into pedigree seed culture medium.
(3) production and culture of culture kind
A. production culture kind fluid nutrient medium: weigh respectively: urea 10g, bagasse 12g, wheat 38g, glucose 9g,
Yeast extract 15g, corn pulp 10g, calcium chloride 10g, ironic citrate 6g, calcium chloride 5g, gypsum 5g mix above-mentioned component, filter residue,
Culture solution is obtained, sterilize 20min under 0.15Pa pressure, takes out cooling to get fluid nutrient medium is arrived；
B. in the inoculating hood that temperature is 20 DEG C, pedigree seed culture medium is added in the fluid nutrient medium, that is, is trained
It feeding kind, will be cultivated in culture kind dislocation culturing room, the temperature of culturing room is 8 DEG C, relative air humidity≤64%.
Through detecting, strain obtained by 1-3 of the embodiment of the present invention, length, thickness, color and growing way are essentially identical, stablize
Property it is high.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (3)
1. a kind of breeding method of hickory chick strain characterized by comprising
(1) production and culture of parent species
A. mother culture media is made:
The raw material group of the mother culture media becomes, based on parts by weight: 120~180 parts of peeled potatoes, glucose 11~18
Part, 12~16 parts of agar, 0.4~0.6 part of potassium dihydrogen phosphate, 0.3~0.5 part of magnesium sulfate, 3~7 parts of corn flour；
By the peeled potatoes stripping and slicing, adds boiling to boil 15~20 minutes, filtered after smashing to pieces, obtain filtrate；Into above-mentioned filtrate
Glucose, agar, potassium dihydrogen phosphate, magnesium sulfate and the corn flour of above-mentioned parts by weight is added, is heated to agar and dissolves completely, from
The heart takes supernatant, the high pressure sterilization 30min under 0.1MPa pressure, takes out cooling to get mother culture media；
B. the culture of parent species: removing stem for robust growth, a Morchella esculenta (L.) Pers sporophore big, that growing way is good in wild environment, remaining
Cap volumetric concentration be 70% alcohol surface sterilization after to be dried, cut the bacterium of 5mm × 5mm and with aseptic water washing
It 2~3 pieces of meat, accesses in the mother culture media, is placed in incubator and cultivates, until growing up to parent species；The temperature of the incubator
It is 16 DEG C~20 DEG C；
(2) production and culture of original seed
A. pedigree seed culture medium is made:
The raw material group of the pedigree seed culture medium becomes, based on parts by weight: 70~80 parts of apple sawdust, 15~20 parts of wheat, stone
Grey 0.5~1.5 part, 0.5~1.5 part of gypsum, 3~7 parts of soil；
The each component of above-mentioned parts by weight is mixed, is added water and stirred, the content for controlling moisture is 60~70%, under 0.2MPa pressure
1.5~2h of high pressure sterilization takes out cooling to get pedigree seed culture medium；
B. the culture of original seed: in inoculating hood, the parent species are inoculated into the pedigree seed culture medium；The temperature of the inoculating hood
It is maintained at 25 DEG C~30 DEG C；
(3) production and culture of culture kind
A. the fluid nutrient medium of kind is cultivated in production:
The raw material group of the pedigree seed culture medium becomes, based on parts by weight, 6~10 parts of urea, 10~12 parts of bagasse, wheat 32
~38 parts, 6~9 parts of glucose, 10~15 parts of yeast extract, 5~10 parts of corn pulp, 8~10 parts of calcium chloride, ironic citrate 3~6
Part, 3~5 parts of calcium chloride, 1~5 part of gypsum；
The each component of above-mentioned parts by weight is mixed, filter residue obtains culture solution, and sterilize 10~20min under 0.15Pa pressure, takes out
It cools down to get fluid nutrient medium is arrived；
B. in inoculating hood, the pedigree seed culture medium is added in the fluid nutrient medium, that is, obtains culture kind；The inoculation
The temperature of case is maintained at 15 DEG C~20 DEG C.
2. the breeding method of hickory chick strain according to claim 1, which is characterized in that further include obtaining step (3)
The culture kind dislocation culturing room in the step of cultivating.
3. the breeding method of hickory chick strain according to claim 2, which is characterized in that the temperature of the culturing room is 8
DEG C~18 DEG C, relative air humidity≤65%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771426A (en) * | 2019-10-21 | 2020-02-11 | 郭红伟 | Stable culture method of phellinus igniarius strains |
| CN111937676A (en) * | 2020-08-11 | 2020-11-17 | 南京康之春生物科技有限公司 | Morchella mycelium culture medium and preparation method thereof |
| CN113243248A (en) * | 2021-06-15 | 2021-08-13 | 重庆市城口县松坤菌草科技开发有限责任公司 | Morchella strain cultivation method |
| CN113455286A (en) * | 2021-06-28 | 2021-10-01 | 齐齐哈尔大学 | Application of high-substrate-concentration fermented corn steep liquor product in cultivation and production of edible fungi |
| CN115039635A (en) * | 2022-06-08 | 2022-09-13 | 甘肃河州云菇农业科技有限公司 | Liquid culture medium for preparing morchella stock culture in high altitude area and stock culture preparation method |
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|---|---|---|---|---|
| CN107227262A (en) * | 2017-07-08 | 2017-10-03 | 香格里拉市加发农业科技有限责任公司 | The breeding method of hickory chick strain |
| CN107311721A (en) * | 2017-07-03 | 2017-11-03 | 株洲市金盟熹农业拓展有限公司 | A kind of complete artificial culture implantation methods of Lactrarius hatsudake |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107311721A (en) * | 2017-07-03 | 2017-11-03 | 株洲市金盟熹农业拓展有限公司 | A kind of complete artificial culture implantation methods of Lactrarius hatsudake |
| CN107227262A (en) * | 2017-07-08 | 2017-10-03 | 香格里拉市加发农业科技有限责任公司 | The breeding method of hickory chick strain |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771426A (en) * | 2019-10-21 | 2020-02-11 | 郭红伟 | Stable culture method of phellinus igniarius strains |
| CN110771426B (en) * | 2019-10-21 | 2021-08-27 | 郭红伟 | Stable culture method of phellinus igniarius strains |
| CN111937676A (en) * | 2020-08-11 | 2020-11-17 | 南京康之春生物科技有限公司 | Morchella mycelium culture medium and preparation method thereof |
| CN113243248A (en) * | 2021-06-15 | 2021-08-13 | 重庆市城口县松坤菌草科技开发有限责任公司 | Morchella strain cultivation method |
| CN113455286A (en) * | 2021-06-28 | 2021-10-01 | 齐齐哈尔大学 | Application of high-substrate-concentration fermented corn steep liquor product in cultivation and production of edible fungi |
| CN115039635A (en) * | 2022-06-08 | 2022-09-13 | 甘肃河州云菇农业科技有限公司 | Liquid culture medium for preparing morchella stock culture in high altitude area and stock culture preparation method |
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Effective date of registration: 20211206 Address after: 211223 Tongxin village, Shiqiu street, Lishui District, Nanjing City, Jiangsu Province Patentee after: Nanjing Golden Seed Technology Co.,Ltd. Address before: No. 3601 Jiangning Road, Nanjing District hirokage 211171 cities in Jiangsu Province Patentee before: NANJING XIAOZHUANG University |