CN111937676A - Morchella mycelium culture medium and preparation method thereof - Google Patents
Morchella mycelium culture medium and preparation method thereof Download PDFInfo
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- CN111937676A CN111937676A CN202010799754.3A CN202010799754A CN111937676A CN 111937676 A CN111937676 A CN 111937676A CN 202010799754 A CN202010799754 A CN 202010799754A CN 111937676 A CN111937676 A CN 111937676A
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- culture medium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention is suitable for the technical field of morel culture, and provides a morel mycelium culture medium and a preparation method thereof, wherein the formula comprises potatoes, glucose, agar, yeast extract, magnesium sulfate and water, the peeled potatoes are put into the water, the weight value A is obtained by weighing, boiling is continued for 30-60 min, juice is obtained by filtration, water is supplemented to restore the weight to the value A, a potato extract is obtained, the glucose, the agar, the yeast extract and the magnesium sulfate are added into the potato extract, the mixture is dissolved by slow fire and is stirred to be dissolved at the same time, a culture medium is obtained, the culture medium is subpackaged in test tubes, the test tubes are sealed, and finally the test tubes are sterilized and are stored in a sterile environment after the sterilization is finished, so that the culture medium to be used is obtained, the material acquisition is simple, the preparation method of the culture medium is simple, and sufficient nutrient substances can be provided for the culture of morel, effectively improving the growth efficiency and quality of the morchella mycelium.
Description
Technical Field
The invention belongs to the technical field of toadstool culture, and particularly relates to a toadstool mycelium culture medium and a preparation method thereof.
Background
Morchella is a precious large perennial medicinal fungus, which is named because most of morchella grows on Morus plants and the fruit body is yellow brown. The medicinal efficacy of morchella is recorded in Ben Cao gang mu and in the property treatise on the bitter taste and cold nature of morchella at the earliest time. Is used for treating symptoms such as dysentery, night sweat, metrorrhagia, rectocele and bloody diarrhea in the traditional Chinese medicine of China. The latest research shows that the morchella esculenta also has unique anticancer efficacy and is a medicinal fungus with the highest effective rate in the current internationally recognized biological cancer treatment medicaments. The main pharmacological active component of the morchella is morchella polysaccharide, the main purpose of artificially culturing the morchella is to obtain the morchella polysaccharide which can be used as a medicine or used for producing a health-care product preparation, and the morchella polysaccharide is generally obtained by a fermentation liquid, a mycelium and a fruiting body extraction way.
Due to the fact that the usage amount of the morchella is increased day by day and south and korean countries, and the wild morchella in China is purchased in a plunder mode, resources of the wild morchella are exhausted; the research on morel in China just starts, and the proper mother culture medium, liquid culture medium formula and artificial cultivation technology for morel are needed to be perfected at the present stage.
Disclosure of Invention
The invention provides a toadstool mycelium culture medium and a preparation method thereof, and aims to solve the problem of providing a foundation for increasing the supply of toadstool mycelium.
The invention is realized in such a way that a morchella mycelium culture medium comprises potato extract, glucose, agar, yeast extract, magnesium sulfate and water.
Preferably, the feed is prepared from the following raw materials in parts by weight: 180-220 parts of potato, 15-25 parts of glucose, 15-25 parts of agar, 1-1.5 parts of yeast extract, 1-1.5 parts of magnesium sulfate and 900-1100 parts of water.
The invention also provides a preparation method of the morchella mycelium culture medium, which comprises the following steps:
s1, putting peeled potatoes into water, weighing to obtain a weight value A, boiling for 30-60 min, filtering to obtain juice, and supplementing water to restore the weight to the value A to obtain a potato extract;
s2, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling with slow fire to dissolve, and stirring to dissolve at the same time to obtain a culture medium;
s3, subpackaging the culture medium in test tubes and sealing;
and S4, sterilizing the test tube, and storing the test tube in an aseptic environment after the sterilization is finished.
Preferably, in step S1, the mass ratio of potato to water is 1: 5.
Preferably, in the step S2, the stirring time is 10-15 min.
Preferably, in step S4, autoclaving is adopted, and the pressure is controlled to be 0.6-0.7 kg, and the time for sterilization is 60-120 min.
Preferably, in step S4, the test tubes are stored in an inclined position.
Compared with the prior art, the invention has the beneficial effects that: the invention relates to a morchella mycelium culture medium and a preparation method thereof, the formula comprises potato, glucose, agar, yeast extract, magnesium sulfate and water, then putting peeled potatoes into water, weighing to obtain a weight value A, boiling for 30-60 min, filtering to obtain juice, supplementing water to restore the weight to the value A to obtain a potato extract, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling with slow fire to dissolve, stirring to dissolve to obtain culture medium, packaging the culture medium in test tube, sealing, sterilizing the test tube, the material of the invention is easy to obtain, the preparation method of the culture medium is simple, and sufficient nutrient substances can be provided for the culture of the morchella, and the growth efficiency and quality of the morchella hyphae are effectively improved.
Drawings
FIG. 1 is a schematic flow chart of a morchella mycelium culture medium and a preparation method thereof.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
Referring to fig. 1, the present embodiment provides a technical solution: a toadstool mycelium culture medium is prepared from the following raw materials in parts by weight: 180g of potato, 15g of glucose, 15g of agar, 1g of yeast extract, 1g of magnesium sulfate and 900g of water.
The medium was then prepared as follows:
s1, taking 180g of peeled potatoes, putting the peeled potatoes into 900g of water, weighing to obtain 1080g of weight value, boiling for 30-60 min, filtering to obtain juice, supplementing water to recover 1080g of weight value, and obtaining 1080g of potato extract.
S2, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling and dissolving with slow fire, and stirring for 10-15 min to obtain the culture medium.
S3, the culture medium is separately loaded in test tubes and sealed.
S4, carrying out high-pressure sterilization on the test tube, controlling the pressure to be 0.6-0.7 kg, and controlling the sterilization time to be 60-120 min.
Then, the culture medium test tube provided by the embodiment is adopted to culture the morchella mycelium, and the steps are as follows:
(1) inoculation: taking out a plurality of test tubes, inoculating morchella esculenta, sealing by a sealing film, and then putting into an incubator for culture.
(2) Hypha growth: the toadstool grows on the culture medium in a symbiotic manner for 10 days, the streak starts to be drawn on the third day of inoculation, and then the streak is drawn once every 24 hours for 6 times; meanwhile, the color, thickness and density of the hyphae and the growth vigor of the colonies are observed.
Example 2
The embodiment provides a technical scheme: a toadstool mycelium culture medium is prepared from the following raw materials in parts by weight: 190g of potato, 15g of glucose, 15g of agar, 1g of yeast extract, 1g of magnesium sulfate and 950g of water.
The medium was then prepared as follows:
s1, taking 190g of peeled potatoes, putting the peeled potatoes into 950g of water, weighing to obtain 1140g of potato extract, boiling for 30-60 min, filtering to obtain juice, and supplementing water to recover the weight to 1140g to obtain 1140g of potato extract.
S2, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling and dissolving with slow fire, and stirring for 10-15 min to obtain the culture medium.
S3, the culture medium is separately loaded in test tubes and sealed.
S4, carrying out high-pressure sterilization on the test tube, controlling the pressure to be 0.6-0.7 kg, and controlling the sterilization time to be 60-120 min.
Then, the culture medium test tube provided by the embodiment is adopted to culture the morchella mycelium, and the steps are as follows:
(1) inoculation: taking out a plurality of test tubes, inoculating morchella esculenta, sealing by a sealing film, and then putting into an incubator for culture.
(2) Hypha growth: the toadstool grows on the culture medium in a symbiotic manner for 10 days, the streak starts to be drawn on the third day of inoculation, and then the streak is drawn once every 24 hours for 6 times; meanwhile, the color, thickness and density of the hyphae and the growth vigor of the colonies are observed.
Example 3
The embodiment provides a technical scheme: a toadstool mycelium culture medium is prepared from the following raw materials in parts by weight: 200g of potato, 20g of glucose, 20g of agar, 1.2g of yeast extract, 1.2g of magnesium sulfate and 1000g of water.
The medium was then prepared as follows:
s1, putting 200g of peeled potatoes into 1000g of water, weighing to obtain a weight value of 1200g, boiling for 30-60 min, filtering to obtain juice, supplementing water to recover the weight to 1200g, and obtaining 1200g of potato extract.
S2, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling and dissolving with slow fire, and stirring for 10-15 min to obtain the culture medium.
S3, the culture medium is separately loaded in test tubes and sealed.
S4, carrying out high-pressure sterilization on the test tube, controlling the pressure to be 0.6-0.7 kg, and controlling the sterilization time to be 60-120 min.
Then, the culture medium test tube provided by the embodiment is adopted to culture the morchella mycelium, and the steps are as follows:
(1) inoculation: taking out a plurality of test tubes, inoculating morchella esculenta, sealing by a sealing film, and then putting into an incubator for culture.
(2) Hypha growth: the toadstool grows on the culture medium in a symbiotic manner for 10 days, the streak starts to be drawn on the third day of inoculation, and then the streak is drawn once every 24 hours for 6 times; meanwhile, the color, thickness and density of the hyphae and the growth vigor of the colonies are observed.
Example 4
The embodiment provides a technical scheme: a toadstool mycelium culture medium is prepared from the following raw materials in parts by weight: 210g of potato, 20g of glucose, 20g of agar, 1.5g of yeast extract, 1.5g of magnesium sulfate and 1050g of water.
The medium was then prepared as follows:
s1, putting 210g of peeled potatoes into 1050g of water, weighing the potatoes to obtain 1260g of potatoes, boiling the water for 30-60 min, filtering the water to obtain juice, and supplementing water to restore the weight of the potatoes to 1260g to obtain 1260g of potato extract.
S2, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling and dissolving with slow fire, and stirring for 10-15 min to obtain the culture medium.
S3, the culture medium is separately loaded in test tubes and sealed.
S4, carrying out high-pressure sterilization on the test tube, controlling the pressure to be 0.6-0.7 kg, and controlling the sterilization time to be 60-120 min.
Then, the culture medium test tube provided by the embodiment is adopted to culture the morchella mycelium, and the steps are as follows:
(1) inoculation: taking out a plurality of test tubes, inoculating morchella esculenta, sealing by a sealing film, and then putting into an incubator for culture.
(2) Hypha growth: the toadstool grows on the culture medium in a symbiotic manner for 10 days, the streak starts to be drawn on the third day of inoculation, and then the streak is drawn once every 24 hours for 6 times; meanwhile, the color, thickness and density of the hyphae and the growth vigor of the colonies are observed.
Example 5
The embodiment provides a technical scheme: a toadstool mycelium culture medium is prepared from the following raw materials in parts by weight: 220g of potato, 25g of glucose, 25g of agar, 1.5g of yeast extract, 1.5g of magnesium sulfate and 1100g of water.
The medium was then prepared as follows:
s1, taking 220g of peeled potatoes, putting the peeled potatoes into 1100g of water, weighing to obtain a weight value of 1320g, boiling for 30-60 min, filtering to obtain juice, supplementing water to recover the weight to 1320g, and obtaining 1320g of potato extract.
S2, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling and dissolving with slow fire, and stirring for 10-15 min to obtain the culture medium.
S3, the culture medium is separately loaded in test tubes and sealed.
S4, carrying out high-pressure sterilization on the test tube, controlling the pressure to be 0.6-0.7 kg, and controlling the sterilization time to be 60-120 min.
Then, the culture medium test tube provided by the embodiment is adopted to culture the morchella mycelium, and the steps are as follows:
(1) inoculation: taking out a plurality of test tubes, inoculating morchella esculenta, sealing by a sealing film, and then putting into an incubator for culture.
(2) Hypha growth: the toadstool grows on the culture medium in a symbiotic manner for 10 days, the streak starts to be drawn on the third day of inoculation, and then the streak is drawn once every 24 hours for 6 times; meanwhile, the color, thickness and density of the hyphae and the growth vigor of the colonies are observed.
Through the observation of the cultivation process of the morchella mycelium, the culture medium provided by the third embodiment has the best growth speed and growth quality of the mycelium in the third embodiment. The growth speed and the growth quality of the morchella mycelium in all the examples are superior to those of the morchella mycelium which naturally grows under ordinary conditions.
In conclusion, the invention adds potato, glucose, agar, yeast extract, magnesium sulfate and water into the culture medium formula, then putting peeled potatoes into water, weighing to obtain a weight value A, boiling for 30-60 min, filtering to obtain juice, supplementing water to restore the weight to the value A to obtain a potato extract, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling with slow fire to dissolve, stirring to dissolve to obtain culture medium, packaging the culture medium in test tube, sealing, sterilizing the test tube, the material of the invention is easy to obtain, the preparation method of the culture medium is simple, and sufficient nutrient substances can be provided for the culture of the morchella, and the growth efficiency and quality of the morchella hyphae are effectively improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. The morchella mycelium culture medium is characterized in that: comprises potato, glucose, agar, yeast extract, magnesium sulfate and water.
2. The morchella mycelium culture medium according to claim 1, wherein: the feed is prepared from the following raw materials in parts by weight: 180-220 parts of potato, 15-25 parts of glucose, 15-25 parts of agar, 1-1.5 parts of yeast extract, 1-1.5 parts of magnesium sulfate and 900-1100 parts of water.
3. The method for preparing a morchella mycelium culture medium according to any one of claims 1 to 2, wherein the culture medium comprises: the method comprises the following steps:
s1, putting peeled potatoes into water, weighing to obtain a weight value A, boiling for 30-60 min, filtering to obtain juice, and supplementing water to restore the weight to the value A to obtain a potato extract;
s2, adding glucose, agar, yeast extract and magnesium sulfate into the potato extract, boiling with slow fire to dissolve, and stirring to dissolve at the same time to obtain a culture medium;
s3, subpackaging the culture medium in test tubes and sealing;
and S4, sterilizing the test tube, and storing the test tube in an aseptic environment after the sterilization is finished.
4. The method for preparing a morchella mycelium culture medium according to claim 3, wherein the culture medium comprises: in step S1, the mass ratio of potato to water is 1: 5.
5. The method for preparing a morchella mycelium culture medium according to claim 3, wherein the culture medium comprises: in step S2, the stirring time is 10-15 min.
6. The method for preparing a morchella mycelium culture medium according to claim 3, wherein the culture medium comprises: in step S4, autoclaving is adopted, the pressure is controlled to be 0.6-0.7 kg, and the sterilization time is 60-120 min.
7. The method for preparing a morchella mycelium culture medium according to claim 3, wherein the culture medium comprises: in step S4, the test tubes are stored in an inclined position.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113755341A (en) * | 2021-09-10 | 2021-12-07 | 忻州师范学院 | Liquid fermentation culture method for morchella |
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| CN102687639A (en) * | 2011-12-19 | 2012-09-26 | 河南科技大学 | Method for separating Morchella strains |
| CN107129938A (en) * | 2017-07-06 | 2017-09-05 | 江油市静远食用菌科技有限责任公司 | It is a kind of to improve the method that morchella mother culture manufactures success rate |
| CN108029454A (en) * | 2017-12-29 | 2018-05-15 | 崔洋 | Indoor cultivation method of morel |
| CN110301291A (en) * | 2018-03-20 | 2019-10-08 | 南京晓庄学院 | A kind of breeding method of hickory chick strain |
| CN110622777A (en) * | 2019-10-23 | 2019-12-31 | 江苏农林职业技术学院 | Culture medium of morchella mother strain and preparation method of morchella mother strain |
-
2020
- 2020-08-11 CN CN202010799754.3A patent/CN111937676A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687639A (en) * | 2011-12-19 | 2012-09-26 | 河南科技大学 | Method for separating Morchella strains |
| CN107129938A (en) * | 2017-07-06 | 2017-09-05 | 江油市静远食用菌科技有限责任公司 | It is a kind of to improve the method that morchella mother culture manufactures success rate |
| CN108029454A (en) * | 2017-12-29 | 2018-05-15 | 崔洋 | Indoor cultivation method of morel |
| CN110301291A (en) * | 2018-03-20 | 2019-10-08 | 南京晓庄学院 | A kind of breeding method of hickory chick strain |
| CN110622777A (en) * | 2019-10-23 | 2019-12-31 | 江苏农林职业技术学院 | Culture medium of morchella mother strain and preparation method of morchella mother strain |
Non-Patent Citations (1)
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| 孙大成等: "羊肚菌人工栽培技术综述", 《黑龙江生态工程职业学院学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113755341A (en) * | 2021-09-10 | 2021-12-07 | 忻州师范学院 | Liquid fermentation culture method for morchella |
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