CN107227262A - The breeding method of hickory chick strain - Google Patents
The breeding method of hickory chick strain Download PDFInfo
- Publication number
- CN107227262A CN107227262A CN201710553458.3A CN201710553458A CN107227262A CN 107227262 A CN107227262 A CN 107227262A CN 201710553458 A CN201710553458 A CN 201710553458A CN 107227262 A CN107227262 A CN 107227262A
- Authority
- CN
- China
- Prior art keywords
- parts
- culture
- test tube
- culture medium
- making
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a kind of breeding method of hickory chick strain, comprise the following steps：1) making and culture of parent species, 2) making and culture of original seed, 3) making and culture of culture kind, the present invention makes an adjustment each culture medium during the production of hybrid seeds, and the preparation method of each culture medium is also all made adjustment, because of the unstability of hickory chick strain, the present invention will add potassium dihydrogen phosphate, magnesium sulfate, vitamin and corn flour in mother culture media, so that the nutriment that parent species are drawn from culture medium is more, by test tube slant gauze moisturizing, it is avoided to be attacked by steam, so as to influence the performance of culture medium；Fluid nutrient medium is made in culture medium, and adds more multistability and the material rich in nutrient, such as urea, wheat bran, it is not easy to be invaded by miscellaneous bacteria, therefore, it can keep the stability of strain, avoid strain display form and female parent in the reproductive process in later stage inconsistent, influence yield.
Description
Technical field
The present invention relates to edible mushroom technical field, more particularly to a kind of breeding method of hickory chick strain.
Background technology
Hickory chick is also known as sheep tripe dish, sheep tripe mushroom etc., and because its cap is uneven, form exactly likes sheep tripe and gained the name.Hickory chick
Pharmaceutically acceptable, its is mild-natured, sweet cold, cures mainly weakness of the spleen and the stomach, indigestion, abundant expectoration shortness of breath, spirit loss etc., with kidney tonifying, establishing-Yang,
Cerebrum tonifying, the effects such as refresh oneself.Medical value is very high.
But in the cultivating process of hickory chick, homocellular hyphal cell performance difference can be run into, in identical conditions
Lower mycelial growth but has different performances, particularly be often inoculated with once it is all different with previous mycelial growth, no matter the length of mycelia
Short, thickness, speed, color, growing way and last performance are all different, even original used, or First Year went out mushroom
Old strain be also that equal humiture and interior external condition are but showed in this way, taking same raw material, formula, operating method
Go out larger gap, therefore, how to cultivate the stable strain of performance so that later product steady quality is particularly important.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of strain properties it is stable, be suitable for the hickory chick bacterium manually cultivated
The breeding method planted.
In order to solve the above-mentioned technical problem, the technical scheme is that：A kind of breeding method of hickory chick strain, including
Following steps：
1) making and culture of parent species
A. mother culture media is made：In terms of parts by weight：100-200 parts of peeled potatoes, 10-20 parts of glucose, agar
10-15 parts, 0.3-0.5 parts of potassium dihydrogen phosphate, 0.2-0.4 parts of magnesium sulfate .2-0.3 parts of vitaminB10,05-17 parts of corn flour,
Above-mentioned component is put into water and heated, until agar boiling completely, is put into centrifuge and centrifuges, take supernatant, produce parent species
Culture medium；
B. the mother culture media made is imported in funnel while hot, dispenses test tube, every batch is used test tube of the same size,
Loading amount is identical, about the 1/4 of test tube length, and after tampon beyond the Great Wall after agar solidification, tampon portion carries out moistureproof work, is placed on height
Press in autoclave and sterilize, it is bundled to put inclined-plane after sterilizing, and cover on inclined-plane several layers of gauze moisturizings, will after after its completely solidification
It is put into each test tube in aseptic inoculation box and accesses mycelia, be placed in incubator, until grows up to parent species；
2) making and culture of original seed
A. pedigree seed culture medium is made：In terms of parts by weight：By 60-70 parts of weed tree sawdust, 10-12 parts of wheat bran, sucrose 10-12
Part, 0.5-0.7 parts of land plaster, 0.2-0.4 parts of calcium superphosphate, 0.5-0.7 parts of soil, 10-12 parts of cotton seed hull, 3-5 parts of wood chip, soil
1-2 parts of earth, 20-35 parts of sterilized water, pH is adjusted to 6-7, above-mentioned component is poured into 3-5min is stirred in blender, by moisture control
System loads blake bottle in 50-55%, and blake bottle is placed in autoclave after sterilizing, takes out cooling and obtains pedigree seed culture medium；
B. blake bottle is put into inoculating hood with the test tube equipped with parent species, the mycelia parent species in test tube is linked into original seed training
In the blake bottle for supporting base, 30 DEG C are maintained the temperature at；
3) making and culture of culture kind
A. the fluid nutrient medium of culture kind is made：In terms of parts by weight, 5-8 parts of urea, 9-10 parts of bagasse, wheat bran 30-
40 parts, 5-10 parts of glucose, 10-15 parts of yeast extract, 5-10 parts of corn steep liquor, 7-9 parts of calcium chloride, 2-8 parts of ironic citrate, calcium chloride
2-6 parts, above-mentioned component is mixed, filter residue obtains nutrient solution, nutrient solution is poured into 500 milliliters of triangular flask by 1-5 parts of land plaster
It is interior, per bottled 100 milliliters of amount, glass fragment of the diameter below 0.8 millimeter is added, is sealed with tampon and brown paper, 1.5 thousand
Sterilize 20-30min under gram/cm pressure, and taking-up is cooled to less than 20-30 DEG C, that is, obtains culture medium；
B. Primary spawn bottle and fluid nutrient medium are placed in inoculating hood and seal half an hour, then original seed is linked into liquid training
Support in base, culture kind can be obtained under 15-20 environment.
Described agar selects cold water soak 20min before mother culture media is made, and picks up and is slowly added into after tearing up.
Step 1) in incubator temperature be maintained at 25-30 DEG C.
The present invention makes an adjustment each culture medium during the production of hybrid seeds, and each culture medium preparation method also all
Make adjustment, because of the unstability of hickory chick strain, the present invention will be added in mother culture media potassium dihydrogen phosphate, magnesium sulfate,
Vitamin and corn flour so that the nutriment that parent species are drawn from culture medium is more, by test tube slant gauze moisturizing, it is to avoid
It is attacked by steam, so as to influence the performance of culture medium；Fluid nutrient medium is made in culture medium, and adds more multistability and richness
Material containing nutrient, such as urea, wheat bran, it is not easy to invaded by miscellaneous bacteria, therefore, it can keep the stability of strain, it is to avoid
Strain display form and female parent in the reproductive process in later stage is inconsistent, influences yield.
Embodiment
The embodiment to the present invention is described further below.Herein it should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but does not constitute limitation of the invention.In addition, invention described below
As long as involved technical characteristic does not constitute conflict and can be just mutually combined each other in each embodiment.
Embodiment 1：
:A kind of breeding method of hickory chick strain, comprises the following steps：
1) making and culture of parent species
A. mother culture media is made：In terms of parts by weight：100-200 parts of peeled potatoes, 10-20 parts of glucose, agar
10-15 parts, 0.3-0.5 parts of potassium dihydrogen phosphate, 0.2-0.4 parts of magnesium sulfate .2-0.3 parts of vitaminB10,05-17 parts of corn flour,
Above-mentioned component is put into water and heated, until agar boiling completely, is put into centrifuge and centrifuges, take supernatant, produce parent species
Culture medium；
B. the mother culture media made is imported in funnel while hot, dispenses test tube, every batch is used test tube of the same size,
Loading amount is identical, about the 1/4 of test tube length, and after tampon beyond the Great Wall after agar solidification, tampon portion carries out moistureproof work, is placed on height
Press in autoclave and sterilize, it is bundled to put inclined-plane after sterilizing, and cover on inclined-plane several layers of gauze moisturizings, will after after its completely solidification
It is put into each test tube in aseptic inoculation box and accesses mycelia, be placed in incubator, incubator temperature is 25-30 degrees Celsius, until
Grow up to parent species；
2) making and culture of original seed
A. pedigree seed culture medium is made：In terms of parts by weight：By 60-70 parts of weed tree sawdust, 10-12 parts of wheat bran, sucrose 10-12
Part, 0.5-0.7 parts of land plaster, 0.2-0.4 parts of calcium superphosphate, 0.5-0.7 parts of soil, 10-12 parts of cotton seed hull, 3-5 parts of wood chip, soil
1-2 parts of earth, 20-35 parts of sterilized water, wherein, described agar selects cold water soak 20min before mother culture media is made, and drags for
Rise and be slowly added into after tearing up.PH is adjusted to 6-7, above-mentioned component is poured into 3-5min is stirred in blender, moisture control is existed
50-55%, loads blake bottle, and blake bottle is placed in autoclave after sterilizing, takes out cooling and obtains pedigree seed culture medium；
B. blake bottle is put into inoculating hood with the test tube equipped with parent species, the mycelia parent species in test tube is linked into original seed training
In the blake bottle for supporting base, 30 DEG C are maintained the temperature at；
3) making and culture of culture kind
A. the fluid nutrient medium of culture kind is made：In terms of parts by weight, 5-8 parts of urea, 9-10 parts of bagasse, wheat bran 30-
40 parts, 5-10 parts of glucose, 10-15 parts of yeast extract, 5-10 parts of corn steep liquor, 7-9 parts of calcium chloride, 2-8 parts of ironic citrate, calcium chloride
2-6 parts, above-mentioned component is mixed, filter residue obtains nutrient solution, nutrient solution is poured into 500 milliliters of triangular flask by 1-5 parts of land plaster
It is interior, per bottled 100 milliliters of amount, glass fragment of the diameter below 0.8 millimeter is added, is sealed with tampon and brown paper, 1.5 thousand
Sterilize 20-30min under gram/cm pressure, and taking-up is cooled to less than 20-30 DEG C, that is, obtains culture medium；
B. Primary spawn bottle and fluid nutrient medium are placed in inoculating hood and seal half an hour, then original seed is linked into liquid training
Support in base, culture kind can be obtained under 15-20 environment.
Embodiment 2：
A kind of breeding method of hickory chick strain, comprises the following steps：
1) making and culture of parent species
A. mother culture media is made：In terms of parts by weight：200 parts of peeled potatoes, 20 parts of glucose, 15 parts of agar, phosphorus
Above-mentioned component is put into water and heated by 0.5 part of acid dihydride potassium, 0.4 part of magnesium sulfate .3 parts of vitaminB10,17 parts of corn flour,
Until agar boiling completely, is put into centrifuge and centrifuges, take supernatant, produce mother culture media；
B. the mother culture media made is imported in funnel while hot, dispenses test tube, every batch is used test tube of the same size,
Loading amount is identical, about the 1/4 of test tube length, and after tampon beyond the Great Wall after agar solidification, tampon portion carries out moistureproof work, is placed on height
Press in autoclave and sterilize, it is bundled to put inclined-plane after sterilizing, and cover on inclined-plane several layers of gauze moisturizings, will after after its completely solidification
It is put into each test tube in aseptic inoculation box and accesses mycelia, be placed in incubator, until grows up to parent species；
2) making and culture of original seed
A. pedigree seed culture medium is made：In terms of parts by weight：By 60-70 parts of weed tree sawdust, 10-12 parts of wheat bran, sucrose 10-12
Part, 0.5-0.7 parts of land plaster, 0.2-0.4 parts of calcium superphosphate, 0.5-0.7 parts of soil, 10-12 parts of cotton seed hull, 3-5 parts of wood chip, soil
1-2 parts of earth, 20-35 parts of sterilized water, pH is adjusted to 6-7, above-mentioned component is poured into 3-5min is stirred in blender, by moisture control
System loads blake bottle in 50-55%, and blake bottle is placed in autoclave after sterilizing, takes out cooling and obtains pedigree seed culture medium；
B. blake bottle is put into inoculating hood with the test tube equipped with parent species, the mycelia parent species in test tube is linked into original seed training
In the blake bottle for supporting base, 30 DEG C are maintained the temperature at；
3) making and culture of culture kind
A. the fluid nutrient medium of culture kind is made：In terms of parts by weight, 8 parts of urea, 10 parts of bagasse, 40 parts of wheat bran, Portugal
10 parts of grape sugar, 15 parts of yeast extract, 10 parts of corn steep liquor, 9 parts of calcium chloride, 8 parts of ironic citrate, 6 parts of calcium chloride, 1-5 parts of land plaster will
Above-mentioned component mixing, filter residue obtains nutrient solution, in the triangular flask that nutrient solution is poured into 500 milliliters, per bottled 100 milliliters of amount, plus
Enter glass fragment of the diameter below 0.8 millimeter, sealed, sterilized under 1.5 kilograms/cm2 pressure with tampon and brown paper
20-30min, taking-up is cooled to less than 20-30 DEG C, that is, obtains culture medium；
B. Primary spawn bottle and fluid nutrient medium are placed in inoculating hood and seal half an hour, then original seed is linked into liquid training
Support in base, culture kind can be obtained under 15-20 environment.
Embodiment 3：
A kind of breeding method of hickory chick strain, comprises the following steps：
1) making and culture of parent species
A. mother culture media is made：In terms of parts by weight：200 parts of peeled potatoes, 20 parts of glucose, 15 parts of agar, phosphorus
Above-mentioned component is put into water and heated by 0.5 part of acid dihydride potassium, 0.4 part of magnesium sulfate, 0.3 part of vitamin B1,17 parts of corn flour,
Until agar boiling completely, is put into centrifuge and centrifuges, take supernatant, produce mother culture media；
B. the mother culture media made is imported in funnel while hot, dispenses test tube, every batch is used test tube of the same size,
Loading amount is identical, about the 1/4 of test tube length, and after tampon beyond the Great Wall after agar solidification, tampon portion carries out moistureproof work, is placed on height
Press in autoclave and sterilize, it is bundled to put inclined-plane after sterilizing, and cover on inclined-plane several layers of gauze moisturizings, will after after its completely solidification
It is put into each test tube in aseptic inoculation box and accesses mycelia, be placed in incubator, incubator temperature is 25-30 degrees Celsius, until
Grow up to parent species；
2) making and culture of original seed
A. pedigree seed culture medium is made：In terms of parts by weight：By 70 parts of weed tree sawdust, 12 parts of wheat bran, 12 parts of sucrose, land plaster
0.7 part, 0.4 part of calcium superphosphate, 0.7 part of soil, 12 parts of cotton seed hull, 5 parts of wood chip, 2 parts of soil, 35 parts of sterilized water, wherein, it is described
Agar make mother culture media before select cold water soak 20min, pick up and be slowly added into after tearing up.PH is adjusted to 6-7,
Above-mentioned component is poured into 3-5min is stirred in blender, by moisture control in 50-55%, load blake bottle, and blake bottle is put
After being sterilized in autoclave, take out cooling and obtain pedigree seed culture medium；
B. blake bottle is put into inoculating hood with the test tube equipped with parent species, the mycelia parent species in test tube is linked into original seed training
In the blake bottle for supporting base, 30 DEG C are maintained the temperature at；
3) making and culture of culture kind
A. the fluid nutrient medium of culture kind is made：In terms of parts by weight, 5-8 parts of urea, 9-10 parts of bagasse, wheat bran 40
Part, 10 parts of glucose, 15 parts of yeast extract, 10 parts of corn steep liquor, 9 parts of calcium chloride, 8 parts of ironic citrate, 6 parts of calcium chloride, land plaster 5
Part, above-mentioned component is mixed, filter residue obtains nutrient solution, in the triangular flask that nutrient solution is poured into 500 milliliters, per the bottled milli of amount 100
Rise, add glass fragment of the diameter below 0.8 millimeter, sealed with tampon and brown paper, in 1.5 kilograms/cm2 pressure
Lower sterilizing 20-30min, taking-up is cooled to less than 20-30 DEG C, that is, obtains culture medium；
B. Primary spawn bottle and fluid nutrient medium are placed in inoculating hood and seal half an hour, then original seed is linked into liquid training
Support in base, culture kind can be obtained under 15-20 environment.
Strain made embodiment 1-3 in the present invention, is compared, stability is high, and per mu yield with common hickory chick strain
It is high.
Claims (3)
1. a kind of breeding method of hickory chick strain, it is characterised in that：Comprise the following steps：
1) making and culture of parent species
A. mother culture media is made：In terms of parts by weight：100-200 parts of peeled potatoes, 10-20 parts of glucose, agar 10-15
Part, 0.3-0.5 parts of potassium dihydrogen phosphate, 0.2-0.4 parts of magnesium sulfate, 0.2-0.3 parts of vitamin B1,05-17 parts of corn flour will be upper
State component and put heating in water into, until agar boiling completely, is put into centrifuge and centrifuges, take supernatant, produce Mother culture
Base；
B. the mother culture media made is imported in funnel while hot, dispenses test tube, every batch is used test tube of the same size, loading amount
It is identical, about the 1/4 of test tube length, after tampon beyond the Great Wall after agar solidification, tampon portion carries out moistureproof work, is placed on high pressure and goes out
Sterilized in bacterium pot, it is bundled to put inclined-plane after sterilizing, and cover on inclined-plane several layers of gauze moisturizings, will be each after after its completely solidification
It is put into test tube in aseptic inoculation box and accesses mycelia, be placed in incubator, until grows up to parent species；
2) making and culture of original seed
A. pedigree seed culture medium is made：In terms of parts by weight：By 60-70 parts of weed tree sawdust, 10-12 parts of wheat bran, 10-12 parts of sucrose, stone
0.5-0.7 parts of cream powder, 0.2-0.4 parts of calcium superphosphate, 0.5-0.7 parts of soil, 10-12 parts of cotton seed hull, 3-5 parts of wood chip, soil 1-2
Part, pH is adjusted to 6-7, above-mentioned component is poured into 3-5min is stirred in blender by 20-35 parts of sterilized water, and moisture control is existed
50-55%, loads blake bottle, and blake bottle is placed in autoclave after sterilizing, takes out cooling and obtains pedigree seed culture medium；
B. blake bottle is put into inoculating hood with the test tube equipped with parent species, the mycelia parent species in test tube is linked into pedigree seed culture medium
Blake bottle in, maintain the temperature at 30 DEG C；
3) making and culture of culture kind
A. the fluid nutrient medium of culture kind is made：In terms of parts by weight, 5-8 parts of urea, 9-10 parts of bagasse, 30-40 parts of wheat bran,
5-10 parts of glucose, 10-15 parts of yeast extract, 5-10 parts of corn steep liquor, 7-9 parts of calcium chloride, 2-8 parts of ironic citrate, calcium chloride 2-6
Part, 1-5 parts of land plaster mixes above-mentioned component, filter residue obtains nutrient solution, in the triangular flask that nutrient solution is poured into 500 milliliters,
Per bottled 100 milliliters of amount, add glass fragment of the diameter below 0.8 millimeter, sealed with tampon and brown paper, 1.5 kilograms/
Sterilize 20-30min under cm2 pressure, and taking-up is cooled to less than 20-30 DEG C, that is, obtains culture medium；
B. Primary spawn bottle and fluid nutrient medium are placed in inoculating hood and seal half an hour, then original seed is linked into fluid nutrient medium
In, culture kind can be obtained under 15-20 DEG C of environment.
2. the breeding method of hickory chick strain as claimed in claim 1, it is characterised in that：Described agar is making parent species training
Cold water soak 20min is selected before supporting base, picks up and is slowly added into after tearing up.
3. the breeding method of hickory chick strain as claimed in claim 1, it is characterised in that：Step 1) in incubator temperature protect
Hold at 25-30 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710553458.3A CN107227262A (en) | 2017-07-08 | 2017-07-08 | The breeding method of hickory chick strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710553458.3A CN107227262A (en) | 2017-07-08 | 2017-07-08 | The breeding method of hickory chick strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107227262A true CN107227262A (en) | 2017-10-03 |
Family
ID=59957259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710553458.3A Pending CN107227262A (en) | 2017-07-08 | 2017-07-08 | The breeding method of hickory chick strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107227262A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108076972A (en) * | 2017-12-21 | 2018-05-29 | 昆明理工大学 | A kind of method for accelerating Morciiella Esculeuta Mycelia growth |
| CN108668784A (en) * | 2018-05-24 | 2018-10-19 | 项韵男 | A kind of mycelial breeding method of polynary strain |
| CN110301291A (en) * | 2018-03-20 | 2019-10-08 | 南京晓庄学院 | A kind of breeding method of hickory chick strain |
| CN113243248A (en) * | 2021-06-15 | 2021-08-13 | 重庆市城口县松坤菌草科技开发有限责任公司 | Morchella strain cultivation method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823429A (en) * | 2012-09-03 | 2012-12-19 | 朱斗锡 | Novel morel cultivation method |
-
2017
- 2017-07-08 CN CN201710553458.3A patent/CN107227262A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102823429A (en) * | 2012-09-03 | 2012-12-19 | 朱斗锡 | Novel morel cultivation method |
Non-Patent Citations (3)
| Title |
|---|
| LEANDRO PAPINUTTI等: "Influence of the carbon source on the growth and lignocellulolytic enzyme production by Morchella esculenta strains", 《JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY》 * |
| 刘作喜等: "羊肚菌栽培新技术与深层发酵技术", 《中国野生植物资源》 * |
| 朱斗锡: "羊肚菌的菌种制作", 《中国食用菌》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108076972A (en) * | 2017-12-21 | 2018-05-29 | 昆明理工大学 | A kind of method for accelerating Morciiella Esculeuta Mycelia growth |
| CN110301291A (en) * | 2018-03-20 | 2019-10-08 | 南京晓庄学院 | A kind of breeding method of hickory chick strain |
| CN110301291B (en) * | 2018-03-20 | 2021-07-13 | 南京晓庄学院 | Method for cultivating morchella strain |
| CN108668784A (en) * | 2018-05-24 | 2018-10-19 | 项韵男 | A kind of mycelial breeding method of polynary strain |
| CN113243248A (en) * | 2021-06-15 | 2021-08-13 | 重庆市城口县松坤菌草科技开发有限责任公司 | Morchella strain cultivation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107227262A (en) | The breeding method of hickory chick strain | |
| CN106472104B (en) | Manufacturing method for phellinus igniarius cultivation | |
| CN106069185A (en) | A kind of breeding method of Morchella esculenta (L.) Pers | |
| CN107299063B (en) | Preparation method of black-skin termitomyces liquid strain | |
| CN110301291B (en) | Method for cultivating morchella strain | |
| CN103858670A (en) | Method for cultivating pleurotus geesteranus | |
| CN105110961A (en) | Liquid fermentation culture medium for shiitake mushrooms and method for producing shiitake mushrooms through the same | |
| CN104945166A (en) | Edible fungus culture medium | |
| CN101696127A (en) | Production process for manufacturing liquid fertilizer by using fish waste | |
| CN108934785B (en) | Liquid strain culture method and cultivation method of boletus nigricans | |
| CN104335820A (en) | Production method of gastrodia elata associated honey fungus strain | |
| CN105733957A (en) | Method for culturing cordyceps militaris with slant solid strains effectively instead of liquid strains | |
| CN107937329A (en) | A kind of method for improving liquid spawn vigor | |
| KR100483333B1 (en) | Production method of the cauliflower mushroom using fermented sawdust | |
| CN107384824A (en) | Stalk rotten agent soon | |
| CN107739224A (en) | It is a kind of quickly to breed fluid nutrient medium of saussurea involucrata mushroom strains and preparation method thereof | |
| CN105255744B (en) | A kind of lucidum strain purification and rejuvenation cultural method and its culture medium | |
| CN104072299A (en) | Flammulina velutiper liquid strain culture medium and preparation method thereof | |
| CN106212051A (en) | A kind of directional fruiting cultural method of Lentinus Edodes | |
| CN107513500A (en) | A kind of preparation method of hickory chick liquid spawn | |
| CN111512887B (en) | Poria cocos planting method using growth regulating composition preparation | |
| CN108522136A (en) | Black collybia albuminosa culture medium and the preparation method and application thereof | |
| CN108207549A (en) | A kind of Chinese torreya growing nursery and culture matrix and preparation method thereof | |
| CN108977476B (en) | Fermentation liquor rich in ergometrine and preparation method thereof | |
| CN111919660A (en) | Cultivation method of termitomyces albuminosus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171003 |