CN111512887B

CN111512887B - Poria cocos planting method using growth regulating composition preparation

Info

Publication number: CN111512887B
Application number: CN202010366034.8A
Authority: CN
Inventors: 贺宗毅; 陈仕江; 涂永勤; 罗昌树; 邢康康
Original assignee: Chongqing Academy of Chinese Materia Medica
Current assignee: Chongqing Academy of Chinese Materia Medica
Priority date: 2020-04-30
Filing date: 2020-04-30
Publication date: 2021-12-21
Anticipated expiration: 2040-04-30
Also published as: CN111512887A

Abstract

The invention relates to the technical field of traditional Chinese medicine cultivation, and discloses a poria cocos planting method using a growth regulating composition preparation. In the invention, as the growth regulating composition preparation is sprayed, the sclerotium of poria cocos can be maturely harvested after being cultivated in a cellar for 55-65 days, the growth cycle of the sclerotium of poria cocos is shortened, the yield of poria cocos is increased, and the contents of pachymaran and triterpenes in poria cocos are increased, so that the quality of poria cocos is improved.

Description

Poria cocos planting method using growth regulating composition preparation

Technical Field

The invention relates to the technical field of traditional Chinese medicine cultivation, in particular to a poria cocos planting method by using a growth regulating composition preparation.

Background

Poria cocos (wolf.) wolf (Schw.) Ryv.et Gilbn.), a saprophytic and parasitizing brown rot fungus belonging to Basidiomycota (Basidiomycota), Hymenomycetes (Hymenomycetes), Aphyllophorales (Aphyloporachs), Polyporaceae (Polipoleae), Poria (Wolfiporia). Poria cocos is usually grown on the root of Pinus (Pinus) plants, and mycelia are twisted into a mass, i.e., thallus. Poria is a traditional medicinal and edible substance, and has effects of calming heart, promoting diuresis, eliminating dampness, and invigorating spleen. Modern pharmacological studies show that the poria cocos has the effects of regulating human immunity, resisting tumors, inflammation, oxidation and bacteria and the like, and the main effective substances exerting biological effectiveness are pachymaran and triterpenes.

Tuckahoe is sweet and light in taste and has moderate nature, is slightly superior to the effects of excreting dampness, strengthening the spleen and calming the heart, has the characteristics of benefiting, not fierce, strengthening the body resistance, eliminating evil and the like, and has the characteristics of 'long-term taking of soul to nourish the mind, no hunger and prolonging life' in the 'Shen nong Ben Cao Jing', various products such as health food, common food and the like prepared by taking tuckahoe as a raw material, various health care concepts, huge market consumption and increasingly prominent supply and elimination demand. In recent years, more and more attention is paid to tuckahoe cultivation, pine wood is selected, prepared and cultivated in 10-12 months in the current year in each production area, pine wood is piled in a kiln before and after the second Ming festival and cultivated in soil after inoculation, and the tuckahoe is harvested in 10-12 months in the current year or 3-4 months in the third year according to the size of tuckahoe knots and is generally harvested after 6-12 months of growth. Therefore, in the traditional poria cocos cultivation method, the growth period of poria cocos is long. In the poria cultivation process, the poria forming speed and the poria size can directly influence the yield of the poria, so that the rapid development of poria sclerotia is promoted, the poria forming speed is accelerated, and the method has important significance for cultivating the poria on a large scale and improving the yield of the poria.

Disclosure of Invention

The invention aims to provide a poria cocos planting method using a growth regulating composition preparation, so as to solve the problem of long growth period of poria cocos in the traditional poria cocos cultivation method.

In order to achieve the purpose, the invention adopts the following technical scheme: a Poria planting method using growth regulating composition preparation comprises the steps of cellar cultivation and growth regulating composition preparation increasing application, wherein the growth regulating composition preparation prepared by mixing brassinolide and EM bacterial liquid is sprayed in the steps of cellar cultivation and growth regulating composition preparation increasing application.

The principle and the advantages of the scheme are as follows: in the steps of pit-entering cultivation and increasing the application of the growth regulating composition preparation, the growth regulating composition preparation prepared by mixing brassinolide and EM bacterial liquid is sprayed, and the growth regulating composition preparation is discovered by the inventor through continuous exploration. Before the inventor finds that the brassinolide and the EM bacterial liquid have unexpected effects in combination, gibberellin, diethyl aminoethyl hexanoate, forchlorfenuron and the like are tried to be applied to the cultivation of the poria cocos, and then gibberellin, diethyl aminoethyl hexanoate, forchlorfenuron and the like are respectively used in combination with the EM bacterial liquid in the cultivation period of the poria cocos, so that the phenomena of increase of the yield of the poria cocos and increase of the content of active ingredients of the poria cocos do not occur. In the exploration process, the inventor finds that spraying of brassinolide and EM bacterial liquid during the poria cocos cultivation period can effectively improve the poria cocos yield and the content of active ingredients of poria cocos, and then, the inventor further discovers that in the growth regulation composition preparation, the biological activity of brassinolide is closely related to the structure of the brassinolide: the brassinolide with high activity must have the following structural characteristics: the ring A/B is trans; ring B contains a 7-lactone and a 6-keto group; ring A has two hydroxyl groups at 2-position and 3-position; hydroxyl is arranged at the 22 position and the 23 position of the side chain; the 24-position of the side chain has 1-2C substituent groups. The EM bacterial liquid is rich in a plurality of beneficial microorganisms such as actinomycetes, bacillus, bacteria, yeast and the like, and can participate in the synthesis and deconstruction of a plurality of metabolites. The brassinolide is used in combination with the EM bacterial liquid, various beneficial microorganisms in the EM bacterial liquid can perform various physiological and biochemical reactions such as deconstruction and synthesis on the brassinolide to generate the high-activity brassinolide, namely the EM bacterial liquid can activate the brassinolide, so that the rapid development of poria sclerotia is promoted, the poria cocos forming speed is accelerated, the growth cycle of the poria sclerotia is shortened, and the yield of poria cocos is improved.

Meanwhile, when the brassinolide is used in combination with the EM bacterial liquid, the activities of squalene synthetase and lanosterol synthetase can be obviously improved, the activity of alpha-phosphoglucomutase (alpha-PGM) is improved, the activity of Phosphoglucomutase (PGI) is inhibited, the squalene synthetase and lanosterol synthetase are key enzymes guiding acetyl coenzyme A to a poria cocos triterpene synthesis path in the metabolic process of poria cocos sclerotium, and the alpha-phosphoglucomutase (alpha-PGM) and the Phosphoglucomutase (PGI) are key enzymes in the polysaccharide synthesis and accumulation process, so that when the brassinolide is used in combination with the EM bacterial liquid, the contents of the poria cocos triterpene compounds and the pachyman can be improved by influencing the activities of the key enzymes, and the quality of poria cocos is improved. In addition, beneficial microorganisms in the EM bacterial liquid have no inhibition effect on the growth of the sclerotium of the poria cocos, and have the effect of antagonizing or inhibiting harmful microorganisms in soil, so that a good growth environment is created for the growth of the sclerotium of the poria cocos.

In conclusion, the growth regulating composition preparation is sprayed before and during the cultivation of the sclerotium of the poria cocos in the cellar, so that the sclerotium of the poria cocos can grow and mature after being cultivated in the cellar for 55-65 days. Moreover, the scheme is simple and easy to implement, is beneficial to popularization and application, and has wide market prospect.

Preferably, as an improvement, the concentration of brassinolide in the formulation of the growth regulating composition is 1.0 x 10^-4-2.0×10^-4The viable bacteria concentration of the mM EM bacterial liquid is 10⁵-10⁶CFU/g。

When the concentration of the brassinolide is too high, the brassinolide is not easy to be completely dissolved, and when the concentration of the brassinolide is too low, the effect on the sclerotia of the poria cocos is not obvious. When the viable bacteria concentration of the EM bacterial liquid is too high, the number of viable bacteria on the culture material after the growth regulating composition preparation is sprayed is too large, and when the viable bacteria concentration of the EM bacterial liquid is too low, the effect on the sclerotium of the poria cocos is not obvious. Thus, the concentration of brassinolide is 1.0X 10^-4-2.0×10^-4The viable bacteria concentration of the mM EM bacterial liquid is 10⁵-10⁶CFU/g is the preferred choice.

Preferably, as an improvement, the spray amount of the growth regulating composition formulation is 50 to 300 mL.

When the spraying amount of the growth regulating composition preparation is too large, there is a problem that the growth regulating composition preparation is wasted, and when the spraying amount of the growth regulating composition preparation is too small, the effect on the sclerotium of poria cocos is limited, so that the spraying amount of the growth regulating composition preparation of 50-300mL is preferred.

Preferably, as an improvement, the steps of pit cultivation and growth regulating composition preparation application are that the growth regulating composition preparation is sprayed at the inoculation position of the sclerotium of the poria cocos.

Compared with the method of directly spraying the growth regulating composition preparation on the soil covered with the tuckahoe strains, the method of spraying the growth regulating composition preparation on the inoculation positions of the tuckahoe sclerotium can ensure the effective amount of the growth regulating composition preparation.

Preferably, as an improvement, in the step of applying the growth regulating composition preparation, the growth regulating composition preparation is sprayed every 20 to 25 days.

The problem of the fault of the growth regulating composition preparation occurs when the interval for spraying the growth regulating composition preparation is too long, and the problem of the excessive spraying amount of the growth regulating composition preparation occurs when the interval for spraying the growth regulating composition preparation is too short, so that the growth regulating composition preparation is preferably sprayed once every 20 to 25 days.

Preferably, as an improvement, in the cellar cultivation step, after the inoculation of the sclerotium of Poria cocos, the sclerotium of Poria cocos is covered with sandy soil with pH of 5.5-6.5, and the covering thickness is 18-22 cm.

The sandy soil has good air permeability and water permeability, and when the pH value of the sandy soil is 5.5-6.5, the release of effective components in the soil and the growth of microorganisms are facilitated, so that the growth and development of sclerotium of poria cocos are facilitated.

Preferably, as an improvement, the method further comprises the step of selecting a mountain cellar for making the cellar before the cellar-entering cultivation step, wherein the cellar is made on a gentle slope mountain at the elevation of 400-.

The mountain land with a certain slope is beneficial to drainage, hypha death caused by water accumulation in the cellar is avoided, the growth of poria cocos sclerotia is facilitated, the slope of the gentle slope mountain land is not too steep, and otherwise the poria cocos sclerotia and soil in the cellar are easy to slip.

Preferably, as an improvement, the method also comprises the step of preparing a culture material before the step of pit entering cultivation, namely, pine sawdust, pine branches and pine block crushed materials are mixed with wheat grains, wheat bran, cottonseed hulls, magnesium sulfate, monopotassium phosphate, cane sugar, gypsum and lime, clear water is added to adjust the water content, and the culture material mass is not dripped.

The culture material in the scheme can provide sufficient nutrients for the sclerotium of the poria cocos after being cultivated in a cellar.

Preferably, as an improvement, the proportion of the culture material is as follows according to the parts by weight: 10-30 parts of pine sawdust crushed material, 10-30 parts of pine branch crushed material, 15-40 parts of pine block crushed material, 15-25 parts of wheat grain, 5-15 parts of wheat bran, 5-10 parts of cottonseed hull, 0.5-1 part of magnesium sulfate, 0.5-1 part of monopotassium phosphate, 0.1-1 part of cane sugar, 0.1-1 part of gypsum and 0.1-0.5 part of lime.

The nutrient structure of the culture material in the scheme is more in line with the nutrient structure required by the growth of sclerotium of poria cocos.

Preferably, as an improvement, the culture medium bags are packaged into culture bags, and the culture bags are sterilized at the temperature of 115 ℃ and 121 ℃ for 16-24 h.

The cultivation bag can be completely sterilized after being sterilized for 16-24h at the temperature of 115-121 ℃, and the problem of incomplete sterilization can not occur, thereby avoiding the problem of pollution of tuckahoe hypha.

Detailed Description

Example 1

In this embodiment, a method for planting poria cocos by using a growth regulating composition preparation includes the steps of:

s1, selecting a tuckahoe plantation: selecting a gentle slope mountain which is not polluted, has good drainage property and a slope of 20 degrees, and making a cellar on the gentle slope mountain at the altitude of 600m, wherein the gentle slope mountain belongs to a wasteland which is not cultivated with poria cocos within three years.

S2, preparation of compost: mixing 10 parts of exposed pine sawdust, 30 parts of pine branches and 30 parts of pine block crushed materials with 20 parts of wheat grains, 10 parts of wheat bran, 8 parts of cottonseed hulls, 0.5 part of magnesium sulfate, 0.5 part of potassium dihydrogen phosphate, 0.2 part of cane sugar, 0.3 part of gypsum and 0.1 part of lime according to the weight part ratio, adding clear water to adjust the water content, holding the culture material mass without dripping, and bagging after adjusting the water content. And (3) placing the bagged cultivation bag into an autoclave, keeping the temperature at 120 ℃ for 16 hours, sterilizing, and naturally cooling the cultivation bag to room temperature after sterilization.

S3, inoculating and spawning the cultivation bags: transferring the culture bag cooled in the step S2 into an inoculation chamber, transferring Poria cocos strains into the culture bag in an aseptic inoculation chamber to complete inoculation of the Poria cocos strains, then putting the inoculated culture bag into a constant-temperature culture chamber, stacking and discharging in a single row, and adjusting the culture temperature to 25 ℃. And (3) periodically checking the growth condition of the poria cocos hyphae in the culture bag during the culture period, finding that the infection phenomenon of the fungus bag needs to be cleared in time, avoiding cross infection, and culturing until the poria cocos hyphae grow to fill the culture bag.

S4, cellar-entering cultivation: before and after the lunar raining season, selecting a day with sunny weather, cutting one side of the cultivation bag in the step S3, inoculating fresh poria cocos sclerotia seeds with the size of 2cm multiplied by 2cm in the current year at the cut of the cultivation bag, enabling the fresh poria cocos sclerotia seeds to be tightly attached to the culture materials, then laterally placing the inoculated cultivation bag in a cellar, spraying a growth regulating composition preparation on the culture materials at the inoculated position, then covering soil with the thickness of about 20cm, and enabling the covered soil to rise so as to be beneficial to drainage. Wherein the growth regulating composition is brassinolide (1.5 × 10)^-4mM) + EM bacterial liquid (5X 10)⁵CFU/g), the spraying amount is 200 mL; the soil was sandy soil, and the pH of the soil was 6.

S5, applying the growth regulating composition preparation: and during the in-cellar cultivation period, spraying the growth regulating composition preparation every 23 days, wherein the spraying method comprises the steps of lightly pulling open the covered soil to expose the culture material or the poria cocos sclerotia, spraying the growth regulating composition preparation to the culture material near the poria cocos sclerotia, and pulling up the soil to cover the poria cocos sclerotia again. Wherein the growth regulating composition is brassinolide (1.5 × 10)^-4mM) + EM bacterial liquid (5X 10)⁵CFU/g), the spraying amount is 200 mL. Meanwhile, whether the sclerotium of the poria cocos generates plant diseases and insect pests is checked regularly, and the sclerotium of the poria cocos is cleaned and prevented in time, so that the plant diseases and insect pests are prevented from being enlarged.

S6, harvesting, processing and storing: the poria cocos sclerotia can be matured after 55 days of growth after cellar cultivation, and the poria cocos sclerotia maturation mark is as follows: when the nutrient materials in the fungus bags are exhausted, the color is changed from pure white to yellow brown to black; the sclerotium of Poria cocos does not crack and grow, and the exodermis of Poria cocos is brown, firm and inelastic, white, tender and brown. The harvested poria cocos sclerotia has large water content, and needs to be cleaned and dried in time, and then is processed and stored after being cut into pieces and the like.

Example 2

The present embodiment is different from embodiment 1 in that: in steps S4 and S5 of this example, the growth regulating composition formulation was brassinolide (2.0X 10)^-4mM) + EM bacterial liquid (10)⁶CFU/g); in step S6, the sclerotium of Poria cocos is cultivated in cellar and then matured after 60 days of growth。

Example 3

The present embodiment is different from embodiment 1 in that: in steps S4 and S5 of this example, the growth regulating composition formulation was brassinolide (1.0X 10)^-4mM) + EM bacterial liquid (10)⁵CFU/g); in step S6, the sclerotium of Poria cocos is cultivated in cellar and then matured after 65 days of growth.

Example 4

The present embodiment is different from embodiment 1 in that: in steps S4 and S5 of this example, the growth regulating composition formulation was brassinolide (1.0X 10)^-4mM) + EM bacterial liquid (5X 10)⁵CFU/g); in step S6, the sclerotium of Poria cocos can be matured after 62 days of growth after cellar cultivation.

Example 5

The present embodiment is different from embodiment 1 in that: in steps S4 and S5 of this example, the growth regulating composition formulation was brassinolide (1.0X 10)^-4mM) + EM bacterial liquid (10)⁶CFU/g); in step S6, the sclerotium of Poria cocos is cultivated in cellar and then matured after 63 days of growth.

Example 6

The present embodiment is different from embodiment 1 in that: in steps S4 and S5 of this example, the growth regulating composition formulation was brassinolide (1.5X 10)^-4mM) + EM bacterial liquid (10)⁵CFU/g); in step S6, the sclerotium of Poria cocos is cultivated in cellar and then matured after 59 days of growth.

Example 7

The present embodiment is different from embodiment 1 in that: in steps S4 and S5 of this example, the growth regulating composition formulation was brassinolide (1.5X 10)^-4mM) + EM bacterial liquid (10)⁶CFU/g); in step S6, the sclerotium of Poria cocos can be matured after 57 days of growth after cellar cultivation.

Example 8

The present embodiment is different from embodiment 1 in that: in steps S4 and S5 of this example, the growth regulating composition formulation was brassinolide (2.0X 10)^-4mM) + EM bacterial liquid (10)⁵CFU/g); in step S6, the sclerotium of Poria cocos is cultivated in cellarThe product can be matured after 64 days of growth.

Example 9

The present embodiment is different from embodiment 1 in that: in steps S4 and S5 of this example, the growth regulating composition formulation was brassinolide (2.0X 10)^-4mM) + EM bacterial liquid (5X 10)⁵CFU/g); in step S6, the sclerotium of Poria cocos can be matured after 61 days of growth after cellar cultivation.

Example 10

The present embodiment is different from embodiment 1 in that: in step S2 of this embodiment, the weight parts of the compost are: 30 parts of pine sawdust, 20 parts of pine branches, 20 parts of pine block crushed matter, 25 parts of wheat grains, 15 parts of wheat bran, 5 parts of cottonseed hulls, 1 part of magnesium sulfate, 1 part of monopotassium phosphate, 1 part of cane sugar, 1 part of gypsum and 0.5 part of lime.

Example 11

The present embodiment is different from embodiment 1 in that: in step S2 of this embodiment, the weight parts of the compost are: 20 parts of pine sawdust, 10 parts of pine branches, 15 parts of pine block crushed matter, 20 parts of wheat grains, 5 parts of wheat bran, 10 parts of cottonseed hulls, 0.6 part of magnesium sulfate, 0.8 part of monopotassium phosphate, 0.5 part of cane sugar, 0.5 part of gypsum and 0.3 part of lime.

Example 12

The present embodiment is different from embodiment 1 in that: in step S4 of this example, the pH of the soil was 7.

Example 13

The present embodiment is different from embodiment 1 in that: in step S4 of this example, the pH of the soil was 5.

In comparative examples 1 to 14 and comparative examples 1 to 3, different spray formulations were used instead of the growth regulating composition preparation of example 1, as shown in table 1, and the rest of the procedure was the same as in example 1.

TABLE 1 spray selection and concentration table for each group

The growth cycle of mature sclerotium of Poria cocos, the yield of Poria cocos, the content of pachymaran and the content of triterpenoids in Poria cocos were recorded in examples 1-9, comparative examples 1-14 and control groups 1-3, wherein the pachymaran content was measured according to the standard "determination of crude polysaccharide content in NY/T1676-.

TABLE 2 influence of each group on Poria growth cycle, Poria yield and active ingredient (mean + -SE, n-10)

Comparing the data of comparative examples 1-12 with the data of comparative examples 1-3, it can be seen that the growth cycle of Poria cocos, the yield of Poria cocos, the content of pachyman and triterpenes do not change significantly when the growth regulating hormones or EM bacterial liquids are sprayed alone, which indicates that the growth of Poria sclerotia is not affected by the growth of Poria cocos sclerotia and the yield or quality of Poria cocos is not affected by the growth regulating hormones or EM bacterial liquids with different dosages.

Comparing the data of examples 10-13 with the data of the control groups 1-3, it can be seen that the ratio of each component of the tuckahoe compost and the selection of the soil pH have no significant influence on the growth cycle of tuckahoe, the yield of tuckahoe and the effective components of tuckahoe within a certain range of values.

Comparing the data of examples 1-9, comparative example 13, comparative example 14 with the data of control groups 1-3, it can be easily found that the growth cycle of Poria cocos, the yield of Poria cocos, the contents of pachymaran and triterpenes are different after different growth regulating hormones are combined with EM bacterial liquid, wherein gibberellin and EM bacterial liquidAfter the liquid combination, the growth cycle of the poria cocos, the yield of the poria cocos, the content of the pachymaran and the content of the triterpenoids do not change significantly compared with the data of the control group (the P value is greater than 0.05 when the comparative example 13 and the control groups 1-3 are subjected to significance analysis respectively), and similarly, the growth cycle of the poria cocos, the yield of the poria cocos, the content of the pachymaran and the content of the triterpenoids do not change significantly compared with the data of the control group when the diethyl aminoethyl hexanoate (DA-6) and the EM bacterial liquid are combined (the P value is greater than 0.05 when the comparative example 14 and the control groups 1-3 are subjected to significance analysis respectively). After the brassinolide is combined with the EM bacterial liquid, the growth cycle of the poria cocos is shortened to 55-65 days, the highest poria cocos yield is 3652 +/-33.94 g, the highest pachymaran content is 1.92 +/-0.21%, and the highest triterpenoid content is 4.67 +/-0.28%, so that the brassinolide is combined with the EM bacterial liquid, the growth cycle of the poria cocos can be shortened, the yield of the poria cocos is improved, the contents of the pachymaran and the triterpenoid can be effectively improved, and the quality of the poria cocos is improved. In addition, it can be seen from table 2 that the combination of different concentrations of brassinolide and different concentrations of EM bacteria liquid have different effects on the growth of poria cocos, wherein brassinolide (1.5 × 10)^-4mM/L) and EM bacterial liquid (5X 10)⁵CFU/g) is best combined.

Based on the conclusion that the combination of brassinolide and EM liquid can improve the effective components in poria cocos, the inventor further finds that the main mechanism influencing the poria cocos quality when the brassinolide and the EM liquid are combined is that the enzyme activities of two key enzymes, namely squalene synthetase and lanosterol synthetase in the poria cocos sclerotium terpene metabolic process are influenced when the brassinolide and the EM liquid are combined, and meanwhile, the enzyme activities of alpha-phosphoglucomutase (alpha-PGM) and phosphoglucomrose isomerase (PGI) are influenced, and specific results are shown in tables 3 and 4.

It is known that squalene synthase is a key enzyme that catalyzes the pyrophosphoric condensation of two molecules of farnesyl ester to produce squalene, which is a common precursor for the biosynthesis of important terpene-based substances such as triterpenes. Therefore, the activity of squalene synthetase directly influences the yield of terpenoid. In addition, a branched pathway involved in the terpene synthesis pathway, namely a mevalonate pathway, is formed through intermediates such as lanosterol and finally through a series of redox and cyclization reactions. The lanosterol synthetase is positioned at the second branch point of the mevalonate pathway and is a key enzyme in the synthesis process of triterpenoids, so the content and activity of the lanosterol synthetase directly influence the yield of the triterpenoids.

The polysaccharide synthesis pathway involves two major branch pathways of glycolysis and polysaccharide synthesis, wherein the glycolysis pathway makes carbon energy advance to the lactic acid metabolism direction, which is not beneficial to the synthesis and accumulation of polysaccharide, and glucose phosphate isomerase (PGI) is a key control enzyme of the glycolysis pathway and dominates the carbon energy advance to the lactic acid metabolism direction. The polysaccharide synthesis pathway is designed to move carbon energy to the direction of accumulation of polysaccharide synthesis, and α -phosphoglucomutase (α -PGM) is a key enzyme in the polysaccharide synthesis pathway and is an important branch point in the pathway. Therefore, the activity of α -phosphoglucomutase (α -PGM) and Phosphoglucomutase (PGI) directly affects the yield of polysaccharide.

TABLE 3 Effect of different groups on the Activity of enzymes critical for the Synthesis of triterpenoids (mean. + -. SE, n ═ 10)

TABLE 4 Effect of different groups on the Activity of the enzymes essential for the Synthesis of pachyman (mean. + -. SE, n ═ 10)

As can be seen from table 3, the activities of squalene synthetase and lanosterol synthetase were not significantly improved by using each growth-regulating steroid hormone or EM bacterial solution alone, and the activities of squalene synthetase and lanosterol synthetase were not improved when gibberellin was used in combination with EM bacterial solution and when diethyl aminoethyl hexanoate was used in combination with EM bacterial solution. However, when the brassinolide is combined with the EM bacterial liquid, the activities of squalene synthetase and lanosterol synthetase in a poria triterpene synthesis pathway can be obviously improved, and the squalene synthetase and the lanosterol synthetase are key enzymes for guiding the poria triterpene synthesis pathway in a poria sclerotium metabolic process, so that when the brassinolide is combined with the EM bacterial liquid, the content of triterpenoids in poria can be improved by improving the activities of the squalene synthetase and the lanosterol synthetase.

In addition, it is found from table 4 that the activity of α -phosphoglucomutase (α -PGM) is not significantly improved or the activity of Phosphoglucomutase (PGI) is not significantly reduced by using each growth-regulating hormone or EM bacterial solution alone, and that the activity of α -phosphoglucomutase (α -PGM) is not improved or the activity of Phosphoglucomutase (PGI) is not reduced when gibberellin is used in combination with EM bacterial solution or when diethyl aminoethyl hexanoate is used in combination with EM bacterial solution. However, when the brassinolide is used in combination with the EM bacterial liquid, the activity of the alpha-phosphoglucomutase (alpha-PGM) is obviously greatly improved, and the activity of the Phosphoglucomutase (PGI) is obviously greatly inhibited, so that when the brassinolide is used in combination with the EM bacterial liquid, the content of pachyman can be increased by increasing the enzyme activity of the alpha-phosphoglucomutase (alpha-PGM) and inhibiting the enzyme activity of the Phosphoglucomutase (PGI) to enable the energy carbon compound to move towards the direction of polysaccharide synthesis and accumulation.

The foregoing is merely an example of the present invention and common general knowledge in the art of designing and/or characterizing particular aspects and/or features is not described in any greater detail herein. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several variations and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims

1. A composition for regulating growthThe poria cocos planting method of the preparation comprises the step of cellar cultivation, and is characterized in that: the method also comprises a step of increasing the growth regulating composition preparation, wherein the steps of pit-entering cultivation and increasing the growth regulating composition preparation are both sprayed with the growth regulating composition preparation prepared by mixing brassinolide and EM bacterial liquid; the concentration of brassinolide in the growth regulating composition preparation is 1.0 × 10^-4-2.0×10^-4The viable bacteria concentration of the mM EM bacterial liquid is 10⁵-10⁶CFU/g; the spraying amount of the growth regulating composition preparation is 50-300 mL.

2. The method for planting poria cocos using a growth regulating composition preparation according to claim 1, wherein: in the steps of cellar cultivation and growth regulating composition preparation increasing, the growth regulating composition preparation is sprayed at the inoculation position of the sclerotium of the tuckahoe.

3. The poria cocos planting method using the growth regulating composition preparation according to claim 1 or 2, wherein: in the step of applying the growth regulating composition preparation, the growth regulating composition preparation is sprayed every 20 to 25 days.

4. The method for planting poria cocos using a growth regulating composition preparation according to claim 3, wherein: in the cellar cultivation step, after inoculating Poria sclerotium, covering Poria sclerotium with sandy soil with pH of 5.5-6.5 to cover thickness of 18-22 cm.

5. The method of claim 4, wherein the composition comprises: before the cellar-entering cultivation step, the method also comprises a step of selecting a mountain cellar for building, wherein the cellar is built on a gentle slope mountain at the elevation of 400-.

6. The method for planting poria cocos using a growth regulating composition preparation according to claim 5, wherein: before the cellar cultivation step, the method also comprises a step of preparing a culture material, wherein pine sawdust, pine branches and pine block crushed substances are mixed with wheat grains, wheat bran, cottonseed hulls, magnesium sulfate, potassium dihydrogen phosphate, cane sugar, gypsum and lime, clear water is added to adjust the water content, and the culture material mass is held without dripping water.

7. The method of claim 6, wherein the composition comprises: the proportion of the culture material is as follows according to the parts by weight: 10-30 parts of pine sawdust crushed material, 10-30 parts of pine branch crushed material, 15-40 parts of pine block crushed material, 15-25 parts of wheat grain, 5-15 parts of wheat bran, 5-10 parts of cottonseed hull, 0.5-1 part of magnesium sulfate, 0.5-1 part of monopotassium phosphate, 0.1-1 part of cane sugar, 0.1-1 part of gypsum and 0.1-0.5 part of lime.

8. The method of claim 7, wherein the composition comprises: and bagging the culture material into a culture bag, and sterilizing the culture bag at the temperature of 115 ℃ and 121 ℃ for 16-24 h.