CN108401788A - A kind of cultural method of Poria cocos - Google Patents
A kind of cultural method of Poria cocos Download PDFInfo
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- CN108401788A CN108401788A CN201810588104.7A CN201810588104A CN108401788A CN 108401788 A CN108401788 A CN 108401788A CN 201810588104 A CN201810588104 A CN 201810588104A CN 108401788 A CN108401788 A CN 108401788A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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Abstract
The present invention relates to a kind of cultural method of Poria cocos, which is implanted into pine tree Duan Muhou in cultigen, is wrapped up the wooden peripheral surface of pine tree section and inoculation cross section with film, make poria cocos sclerotium can only never mantle cross section growth；And pine tree section wood is tilted and is covered again with soil after putting, to make poria cocos sclerotium that can only can be mushroomed out in the environment of its biological characteristics of optimum in the wooden cross section fixed point knot Siberian cocklebur of the pine tree section of non-wrap film.Cultural method knot Siberian cocklebur controllable quantity of the present invention, high at Siberian cocklebur quality, high product rate is high；Draw grafting without lure, saves fresh Poria cocos and labour's expense.
Description
Technical field
The present invention relates to the cultural methods of Chinese medicine, are specifically related to a kind of cultural method of Poria cocos.
Background technology
Poria cocos is that China's tradition commonly uses integration of drinking and medicinal herbs Chinese medicine.It is widely used in tcm clinical practice formula, Chinese patent drug, health food
Raw material and herbal cuisine raw material.The base of Poria cocos is On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf, medicinal part
For its sclerotium, medicinal material is formed through peeling, cutting, drying.
The theoretical foundation of Poria cocos cultivation：
(1) life habit of Poria cocos bacterium：Mycelia absorbs moisture and nutrition from wood surface, while secretase decomposes and turns
Change the cellulose in timber, hemicellulose, lignin and seeks saprogenesis as nutrient source；
(2) formation mechenism of sclerotium：By a large amount of mycelium by aggregation, knot, is formed " mycelium agglomerate ", then converted
Sclerotium " former base " is formed, and the nutriment of mycelium conversion is received, accumulated with this, gradually increases, develop.
Poria cocos main producing region Poria cocos cultural method at present：
(1) Yunnan, Guizhou producing region：Felling pine tree trunk makees compost, and pine forest periphery sandy loam is selected to make cultivation field；
It cultivates and digs cellar for storing things on field, Duan Mu used commonly reaches 30 kilograms or so, and by section wood embedment cellar for storing things, user's work point is from cultivation
" poria cocos strains " introduces a collection, is inoculated on Duan Mu, then earthing, heat and moisture preserving, and the Poria cocos mycelia of " strain " is promoted to rejuvenate simultaneously
On elongation growth to Duan Mu, when Poria mycelium mass propagation, aggregated, knot forms newborn sclerotium.This cultural method
Common Cultivation can once harvest Poria cocos two to three times, and yield is higher, but, Poria cocos small, growth site more in the presence of knot Siberian cocklebur quantity
Arbitrarily, the shortcomings of commodity rate is low, the wooden waste of section is serious.
(2) Hubei, Anhui producing region：Felling pine tree trunk makees compost, and pine forest periphery sandy loam is selected to make cultivation field；
It cultivates and digs cellar for storing things on field, section wood is embedded in cellar for storing things, " Poria cocos bacterium of user's work point from cultivation by general 8 kilograms or so of section used wood
Kind " introduces a collection, it is inoculated on Duan Mu, then earthing, heat and moisture preserving, promotes the Poria cocos mycelia of " strain " to restore viability and extend
It grows on Duan Mu, when mycelia covers with Duan Muhou, need to be lured in conjunction with Poria cocos and draw cultivation technique and Poria cocos is double draws method cultivation technique and formed
Newborn sclerotium.Once only harvesting is primary for this cultivation method, has a big, production site of knot Siberian cocklebur controllable quantity, Poria cocos
Fixed, the features such as commodity rate is high, but draw method cultivation technique because it uses to lure and (wait for section after Poria cocos section wood is inoculated with and is embedded to underground
Wood excavates the soil around Duan Mu after covering with mycelia, then with the fresh Poria cocos planted in advance lure and draws grafting), cause fresh
Poria cocos consumption is big, the amount of labour used is big.
Invention content
In view of this, it is necessary to which there is knot for current Yunnan, Guizhou main producing region Poria cocos cultural method, Siberian cocklebur quantity is more, Poria cocos
The problems such as a small, growth site is random, commodity rate is low, the wooden waste of section is serious and Hubei, Anhui main producing region Poria cocos cultural method
There are fresh Poria cocos consumptions it is big, the amount of labour used is big the problems such as, a kind of cultural method of Poria cocos is provided.
The cultural method of Poria cocos of the present invention, includes the following steps：
Step 1) Mother culture
The Poria cocos meat in-between as the Poria cocos of strain is taken out, Poria cocos meat is accessed on culture medium one, and moves into 22~25
Culture 5-7 days is carried out in DEG C environment, obtains parent species mycelia；
Step 2) expands culture
The access of parent species mycelia is loaded on the sawdust medium one in original seeds bottle, and moves into 20~28 DEG C of environment and is trained
It supports 20-25 days, until mycelia covers with original seeds bottle and obtains original seed mycelia；
The access of original seed mycelia is loaded on the sawdust medium two in cultivating bag, and moves into 20~28 DEG C of environment and is trained
It supports 20-25 days, until mycelia covers with cultivating bag and obtains cultigen；
Step 3) is inoculated with
By on the cross section of cultigen implantation pine tree Duan Mu, with film by the wooden peripheral surface of pine tree section and inoculation cross section into
Row package；Pine tree section wood is tilted, pine tree section wood is covered with soil after putting, wherein the one of pine tree section wood implantation cultigen
End is located at lower section, and it is 20~30 DEG C to keep the temperature of soil, and water content is 20~40%；
Poria cocos maturation can be harvested.
It is implanted into pine tree Duan Muhou in cultigen, the wooden peripheral surface of pine tree section and inoculation cross section are wrapped up with film,
Make poria cocos sclerotium can only never mantle cross section growth；And pine tree section wood is tilted and is covered again with soil after putting, from
And make poria cocos sclerotium can only be in the wooden cross section fixed point knot Siberian cocklebur of the pine tree section of non-wrap film, in the ring of its biological characteristics of optimum
It can be mushroomed out under border.
The culture medium one includes the material component of following weight proportion as a preferred technical solution,：200 matter of potato
Measure part；30 mass parts of glucose；20 mass parts of agar；1 mass parts of potassium dihydrogen phosphate；0.3 mass parts of magnesium sulfate；Ferrous sulfate
0.5 mass parts；1000 mass parts of water.
The sawdust medium one, sawdust medium two include following weight proportion as a preferred technical solution,
Material component：78 parts by weight of sawdust；20 parts by weight of wheat bran or rice bran；1 parts by weight of sucrose；1 parts by weight of gypsum；60 mass parts of water.
The film is polypropylene film or polyethylene film as a preferred technical solution, and the thickness of film is
0.05mm。
As a preferred technical solution, in step 3), when pine tree section wood inclination is put, the angle of pine tree Duan Muyu horizontal planes
It is 15 °.
As a preferred technical solution, in step 3), the soil thickness covered on pine tree Duan Mu is 15cm.
Compared with the prior art, the present invention has following advantageous effect：
1, Siberian cocklebur controllable quantity is tied, high at Siberian cocklebur quality, high product rate is high；
2, draw grafting without lure, save fresh Poria cocos and labour's expense.
Specific implementation mode
In order to facilitate the understanding of those skilled in the art, further being retouched to the present invention below in conjunction with specific embodiment
It states.
A kind of cultural method of Poria cocos comprising following steps：
Step 1) Mother culture
After being cleaned and being sterilized as the Poria cocos surface of strain, in-between Poria cocos meat is taken to access on culture medium one,
And move into 22~25 DEG C of environment and carry out culture 5-7 days, obtain parent species mycelia；Parent species mycelia is positioned in 4 DEG C of environment and is stored.
In the present embodiment, the Poria cocos as strain is to choose single weight October from the Poria cocos of stable high yield just harvested
2.0 kilograms or more of fresh Poria cocos, sclerotium is close to spherical shape, crust is relatively thin, color is faint yellow or brownish red, it is vigorous, outer to grow
Table has that apparent crackle, internal Siberian cocklebur meat is white, juice is more, strong flavor, crust are damaged without insect bite.
It is to the operation cleaned and sterilized as the Poria cocos surface of strain：By as the Poria cocos of strain be rinsed with water to
Without silt, Poria cocos surface is dried with gauze, then carries out surface sterilization with 50~70% alcohol rinses in transfer room, then with sterile
Water is rinsed 3-4 times to remove alcohol.
Step 2) expands culture
The access of parent species mycelia is loaded on the sawdust medium one in original seeds bottle, and moves into 20~28 DEG C by 2 months of second year
Culture 20-25 days is carried out in environment, until mycelia covers with original seeds bottle and obtains original seed mycelia；
The access of original seed mycelia is loaded on the sawdust medium two in cultivating bag, and moves into 20~28 DEG C of environment and is trained
It supports 20-25 days, until mycelia covers with cultivating bag and obtains cultigen.
The culture medium one includes the material component of following weight proportion：200 mass parts of potato；30 mass of glucose
Part；20 mass parts of agar；1 mass parts of potassium dihydrogen phosphate；0.3 mass parts of magnesium sulfate；0.5 mass parts of ferrous sulfate；1000 matter of water
Measure part.
The sawdust medium one, sawdust medium two include the material component of following weight proportion：78 weight of sawdust
Part；20 parts by weight of wheat bran or rice bran；1 parts by weight of sucrose；1 parts by weight of gypsum；60 mass parts of water.
The access of parent species mycelia is gone forward loaded on the sawdust medium one in original seeds bottle, is needed to sawdust medium one and original seed
Bottle carries out steam sterilizing, and sterilising conditions are 110~125 DEG C of temperature, and the duration is 20~30 minutes；
The access of original seed mycelia is gone forward loaded on the sawdust medium two in cultivating bag, is needed to sawdust medium two and cultivation
Bag carries out steam sterilizing, and sterilising conditions are 110~125 DEG C of temperature, and the duration is 20~30 minutes.
Step 3) is inoculated with
It ditches to dig cellar for storing things or do furrow on the paddy field for excavated gutter along slope aspect in well-drained hillside and digs cellar for storing things, mountain
The soil in slope or paddy field is preferably mixed containing sand yellow soil or by yellow clay and fine sand, and the volume ratio of yellow clay and fine sand is
1：2 or 2：1, soil mixes prevention termite medicine, to prevent termite to sting pine tree Duan Mu；
Cultigen is implanted on the cross section of pine tree Duan Mu, the polypropylene film or polyethylene film that are 0.05mm with thickness
The wooden peripheral surface of pine tree section and inoculation cross section are wrapped up；Pine tree section wood is tilted and is placed in cellar for storing things and with soil to pine tree section
Wood is covered, and one end of wherein pine tree section wood implantation cultigen is located at lower section, and the thickness of soil layer covering is 15cm, and top is put up
Mat shelter, it is 20~30 DEG C to keep the temperature of soil, and water content is 20~40%.
When pine tree section wood inclination is put, the angle of pine tree Duan Muyu horizontal planes is 15 °.
The preparation method of the pine tree Duan Mu is：
In in halcyon days 3 days, choosing the pine tree of growth 15 years and cutting down, the branch in addition to the small branches and leaves in top is removed；Through
After crossing natural drying in 3~9 days, part bark is removed longitudinally spacedly；After drying 10~20 days, it is 35 that pine tree, which is sawn into length,
The Duan Mu of~40cm, by " well " font stacking in dry area without shade, and in being capped forage on section grillage, rainy season must cover plastics
Film；It waits for that the section wood in April of second year reaches specified mass dryness fraction, that is, dries to cracked.
The pine tree is one kind in black pine, Chinese pine, Korean pine, Huashan pine, pinus yunnanensis, pinus khasys, masson pine.
Step 4) daily management
7~10 days after inoculation, pine tree section wood can grow the Poria cocos mycelia of white, bacterium feelings at this moment should be checked, so as to timely
It fills a vacancy；It if it find that termite, should be sprayed at termite with prevention termite medicine or ant road, be prevented immediately；
1-2 months after inoculation, loses basal plane and grow mycelia, also not smelling has Poria cocos smell, to reseed.Reseed be will be on not
The pine tree Duan Mu of bacterium is dug out, and dries moisture, the cross section for being inoculated with cultigen is cut again, then connect cross section again；
After Poria cocos forms sclerotium (knot Siberian cocklebur), such as finding that Poria cocos bassets should earth up and weeding in time, and rainy season is it is noted that row
Water.
Poria cocos generally 6 months maturations after inoculation, can be harvested after ripe.
Some embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of cultural method of Poria cocos, which is characterized in that include the following steps：
Step 1) Mother culture
The Poria cocos meat in-between as the Poria cocos of strain is taken out, Poria cocos meat is accessed on culture medium one, and moves into 22~25 DEG C of rings
Culture 5-7 days is carried out in border, obtains parent species mycelia；
Step 2) expands culture
The access of parent species mycelia is loaded on the sawdust medium one in original seeds bottle, and moves into 20~28 DEG C of environment and is cultivated
20-25 days, until mycelia covers with original seeds bottle and obtains original seed mycelia；
The access of original seed mycelia is loaded on the sawdust medium two in cultivating bag, and moves into 20~28 DEG C of environment and is cultivated
20-25 days, until mycelia covers with cultivating bag and obtains cultigen；
Step 3) is inoculated with
By on the cross section of cultigen implantation pine tree Duan Mu, the wooden peripheral surface of pine tree section and inoculation cross section are wrapped with film
It wraps up in；Pine tree section wood is tilted, pine tree section wood is covered with soil after putting, wherein one end position of pine tree section wood implantation cultigen
In lower section, it is 20~30 DEG C to keep the temperature of soil, and water content is 20~40%；
Poria cocos maturation can be harvested.
2. cultural method according to claim 1, it is characterised in that：The culture medium one includes the original of following weight proportion
Expect component
200 mass parts of potato；30 mass parts of glucose；20 mass parts of agar；1 mass parts of potassium dihydrogen phosphate；0.3 matter of magnesium sulfate
Measure part；0.5 mass parts of ferrous sulfate；1000 mass parts of water.
3. cultural method according to claim 1, it is characterised in that：The sawdust medium one, sawdust medium two are equal
Include the material component of following weight proportion
78 parts by weight of sawdust；20 parts by weight of wheat bran or rice bran；1 parts by weight of sucrose；1 parts by weight of gypsum；60 mass parts of water.
4. cultural method according to claim 1, it is characterised in that：The film is that polypropylene film or polyethylene are thin
The thickness of film, film is 0.05mm.
5. cultural method according to claim 1, it is characterised in that：In step 3), when pine tree section wood inclination is put, pine tree
The angle of Duan Muyu horizontal planes is 15 °.
6. cultural method according to claim 1, it is characterised in that：In step 3), the soil layer covered on pine tree Duan Mu is thick
Degree is 15cm.
7. cultural method according to claim 1, it is characterised in that：In step 1), take out as strain Poria cocos wherein
Between Poria cocos meat before, Poria cocos surface is cleaned and is sterilized；Cleaning and the operation sterilized are
It will be rinsed with water to no silt, be rinsed with 50~70% alcohol after drying Poria cocos surface, so as the Poria cocos of strain
It is rinsed afterwards with sterile water to remove alcohol.
8. cultural method according to claim 1, it is characterised in that：In step 2), the access of parent species mycelia is loaded on original seed
Sawdust medium one in bottle is gone forward, and steam sterilizing is carried out to sawdust medium one and original seeds bottle, sterilising conditions be temperature 110~
125 DEG C, the duration is 20~30 minutes；
In step 2), the access of original seed mycelia is gone forward loaded on the sawdust medium two in cultivating bag, to sawdust medium two and is planted
It trains bag and carries out steam sterilizing, sterilising conditions are 110~125 DEG C of temperature, and the duration is 20~30 minutes.
9. according to claim 1 to 8 any one of them cultural method, it is characterised in that：The preparation method of the pine tree Duan Mu
For
In in halcyon days 3 days, choosing the pine tree of growth 14~16 years and cutting down, the branch in addition to the small branches and leaves in top is removed；Through
After crossing natural drying in 3~9 days, part bark is removed longitudinally spacedly；After drying 10~20 days, it is 35 that pine tree, which is sawn into length,
The Duan Mu of~40cm, by " well " font stacking in dry area without shade, and in being capped forage on section grillage, section wood is dried to splitting
Line.
10. cultural method according to claim 9, it is characterised in that：The pine tree is black pine, Chinese pine, Korean pine, the Huashan
It is one or more in pine, pinus yunnanensis, pinus khasys, masson pine.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168969A (en) * | 2018-09-28 | 2019-01-11 | 福建农林大学 | A kind of bamboo Poria cocos cultural method |
| CN111512887A (en) * | 2020-04-30 | 2020-08-11 | 重庆市中药研究院 | Poria cocos planting method using growth regulating composition preparation |
| CN113383674A (en) * | 2021-07-07 | 2021-09-14 | 云茯苓普洱有限公司 | Planting method of poria cocos |
| CN113383673A (en) * | 2021-07-07 | 2021-09-14 | 云茯苓普洱有限公司 | Preparation method of poria cocos planting nutrition bag |
| CN113383672A (en) * | 2021-07-07 | 2021-09-14 | 云茯苓普洱有限公司 | Poria cocos loose pile planting method |
| CN113711848A (en) * | 2021-09-14 | 2021-11-30 | 安庆师范大学 | Mixed soil for cultivating poria cocos and poria cocos cultivation method |
| CN113796260A (en) * | 2021-09-14 | 2021-12-17 | 安庆师范大学 | Poria (Wolfiporia cocos) YX1, and culture medium and cultivation method thereof |
| CN115152530A (en) * | 2022-08-22 | 2022-10-11 | 湖南省林业科学院 | Treatment method of pine wood nematode infected wood |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168969A (en) * | 2018-09-28 | 2019-01-11 | 福建农林大学 | A kind of bamboo Poria cocos cultural method |
| CN111512887A (en) * | 2020-04-30 | 2020-08-11 | 重庆市中药研究院 | Poria cocos planting method using growth regulating composition preparation |
| CN111512887B (en) * | 2020-04-30 | 2021-12-21 | 重庆市中药研究院 | Poria cocos planting method using growth regulating composition preparation |
| CN113383674A (en) * | 2021-07-07 | 2021-09-14 | 云茯苓普洱有限公司 | Planting method of poria cocos |
| CN113383673A (en) * | 2021-07-07 | 2021-09-14 | 云茯苓普洱有限公司 | Preparation method of poria cocos planting nutrition bag |
| CN113383672A (en) * | 2021-07-07 | 2021-09-14 | 云茯苓普洱有限公司 | Poria cocos loose pile planting method |
| CN113711848A (en) * | 2021-09-14 | 2021-11-30 | 安庆师范大学 | Mixed soil for cultivating poria cocos and poria cocos cultivation method |
| CN113796260A (en) * | 2021-09-14 | 2021-12-17 | 安庆师范大学 | Poria (Wolfiporia cocos) YX1, and culture medium and cultivation method thereof |
| CN113796260B (en) * | 2021-09-14 | 2022-08-30 | 安庆师范大学 | Poria (Wolfiporia cocos) YX1, and culture medium and cultivation method thereof |
| CN115152530A (en) * | 2022-08-22 | 2022-10-11 | 湖南省林业科学院 | Treatment method of pine wood nematode infected wood |
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