CN113383674A - Planting method of poria cocos - Google Patents
Planting method of poria cocos Download PDFInfo
- Publication number
- CN113383674A CN113383674A CN202110764632.5A CN202110764632A CN113383674A CN 113383674 A CN113383674 A CN 113383674A CN 202110764632 A CN202110764632 A CN 202110764632A CN 113383674 A CN113383674 A CN 113383674A
- Authority
- CN
- China
- Prior art keywords
- poria cocos
- planting
- bag
- parts
- hyphae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/30—Accessories for use before inoculation of spawn, e.g. sterilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/66—Cultivation bags
Abstract
The invention discloses a planting method of poria cocos, and relates to the technical field of planting of poria cocos. The method comprises the following steps: s1, selecting strains; s2, culturing hyphae of the stock; s3, cultivating hyphae of the cultivated species; s4, guiding wood to select; s5, preparing cultivars; s6, preparing a nutrition bag; s7, inoculating and culturing the nutrition bag; s8, soil preparation and land selection; s9, grafting; s10, planting; and S11, performing later management. According to the planting method of the poria cocos, original strain hypha and cultivated strain hypha are cultured, and the strain is transferred to pine branches for culture after being propagated into hypha, so that nutrition of the pine branches is effectively utilized, the survival rate of the strain is high, the emergence rate and the emergence rate are high, the yield is high, selective material taking is achieved, a pure natural environment is also eliminated, the cost and the labor amount are reduced, meanwhile, insect killing treatment is effectively carried out, and the survival rate of the cultivated poria cocos and the quality of the grown poria cocos are improved.
Description
Technical Field
The invention relates to the technical field of tuckahoe planting, in particular to a planting method of tuckahoe.
Background
Poria is a common Chinese medicinal material, is the dried sclerotium of Poria cocos (a fungus of Polyporaceae), and has pharmacological effects: the traditional Chinese medicine has the effects of promoting diuresis and excreting dampness, strengthening spleen and reducing phlegm, calming heart and tranquilizing mind, detoxifying and resisting cancer, has mild drug properties, can promote diuresis without hurting vital qi, can be used as a good nursing product in spring and summer humid seasons, can treat symptoms such as dysuria, edema and fullness, phlegm retention and cough, vomiting, pernicious vomiting, diarrhea, spermatorrhea, stranguria with turbid urine, palpitation and amnesia, contains pachymic acid which has the effects of enhancing immunity, resisting tumors, tranquilizing mind, reducing blood sugar and the like, can relax smooth muscles of digestive tracts, inhibits gastric acid secretion, prevents hepatocyte necrosis, resists bacteria and the like.
The traditional poria cocos planting and cultivating method generally selects geographical positions such as outdoor hillsides, the planting and cultivating period of the poria cocos is generally long, so that the poria cocos planting and cultivating method is inconvenient for workers to check, is easily influenced by natural weather, is inconvenient for controlling the temperature and humidity of poria cocos planting, is easily influenced by natural insect pests, has low survival rate, causes poor quality and limited production of the poria cocos planted and cultivated, and solves the problems by providing the poria cocos planting method.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a planting method of poria cocos, and aims to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a planting method of poria cocos comprises the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, inoculating the poria cocos flesh in the poria cocos blocks to a culture medium I in a triangular flask, transferring the poria cocos blocks to a thermostat with the temperature of 22-25 ℃ for culturing for 5-7 days until mycelium grows in a culture container, and obtaining required stock hypha;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn hyphae prepared in the step S2 onto a culture medium II in a polypropylene cultivation bag with the diameter of 6cm, and performing cultivation operation until the cultivation bag is full of poria cocos hyphae, so as to obtain cultivation spawn hyphae;
s4, wood guiding operation: peeling pine tree branches with the diameter of 3-5cm and the length of 30-40cm, air-drying, soaking in saturated brown sugar water for a day and a night, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag of the hyphae of the cultivated species obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, and culturing and propagating at the temperature of 15-30 ℃ for 10-15 days for later use;
s6, preparing a nutrition package: mixing pine sawdust, pine wood chips, pine hair, corn flour, wheat bran, brown sugar, gypsum, soybean meal and other raw materials in proportion, sterilizing at the high temperature of 121 ℃ for 4 hours, and cooling for later use;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the S5 step into the nutrition bag prepared in the S6 step under aseptic conditions, and culturing at 15-30 deg.C for 30-40 days.
S8, soil preparation and land selection: selecting a planting field with good drainage, excavating cultivation pits in the planting field in sandy soil with the soil mainly comprising yellow sandy soil and the pH value of 3-5, and spraying a sterilizing liquid to the cultivation pits to perform disinfection and insect killing treatment after excavation;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken, inoculated on the cross section of the leading wood and wrapped by an agricultural film.
S10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, the growth conditions of hyphae in each section are checked so as to supplement the seeds in time, if the poria cocos is exposed out of the ground, soil covering, weeding and insect killing are carried out in time, and drainage is carried out in rainy season.
Further optimizing the technical scheme, the first culture medium in the step S2 comprises 80-120 parts by weight of sweet potato, 30-40 parts by weight of glucose, 20-25 parts by weight of agar, l-3 parts by weight of monopotassium phosphate, 0.3-1 part by weight of magnesium sulfate and 0.5-2 parts by weight of ferrous sulfate, and water is added for mixing to ensure that the water content is 50-60%.
Further optimizing the technical scheme, the first culture medium prepared in the step S2 is sterilized by steam for 20-30min at the temperature of 110-125 ℃.
Further optimizing the technical scheme, in the step S3, the second culture medium is 10-18 parts of gypsum powder, 35-45 parts of corn straw, 6-10 parts of water and 5-10 parts of soybean meal, and the mixture is treated to ensure that the water content is 55-75%.
Further optimizing the technical scheme, the culture medium II prepared in the step S3 is sterilized by steam for 15-25min at the temperature of 110-120 ℃.
Further optimizing the technical scheme, in the step S3, the culture bag is tightened, placed in a sterile environment, cultured at a constant temperature of 10-15 ℃ for 4-10 days, then cultured at a constant temperature of 20-30 ℃ for 15-20 days until the culture bag is full of poria cocos mycelia, and then the mycelium of the culture strain is obtained.
Further optimizing the technical scheme, 65-70% of moisture is reserved in the pine branches which are peeled and air-dried in the step S4.
Further optimizing the technical scheme, an opening is cut through the agricultural film in the step S9, and the thickness of the covering soil above the tree section in the step S11 is 1-5 cm.
Further optimizing the technical scheme, the soil layer is kept moist in the process of timely covering soil in the step S11, and the termite killing medicine for killing insects in the step S8 is used for killing insects.
Compared with the prior art, the invention provides a planting method of poria cocos, which has the following beneficial effects:
1. according to the planting method of the poria cocos, original strain hypha and cultivated strain hypha are cultured, and the strain is transferred to pine branches for culture after being propagated into hypha, so that nutrition of the pine branches is effectively utilized, the survival rate of the strain is high, the emergence rate and the emergence rate are high, the yield is high, selective material taking is achieved, a pure natural environment is also eliminated, the cost and the labor amount are reduced, meanwhile, insect killing treatment is effectively carried out, and the survival rate of the cultivated poria cocos and the quality of the grown poria cocos are improved.
2. According to the planting method of the poria cocos, the culture bags are used for matched culture in the culture process, the production quality and the production yield of the poria cocos in the culture process are improved, the soil culture time is reduced, and the planting period of the poria cocos is greatly shortened compared with that of the traditional planting method.
Drawings
Fig. 1 is a schematic flow chart of a poria cocos planting method provided by the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: referring to fig. 1, the present invention discloses a planting method of poria cocos, comprising the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, taking out the poria cocos meat in the middle of the poria cocos blocks, inoculating the poria cocos blocks onto a first culture medium in a triangular flask, adding water into the first culture medium, mixing the first culture medium with 100 parts by weight of sweet potatoes, 35 parts by weight of glucose, 20 parts by weight of agar, 1 part by weight of monopotassium phosphate, 0.8 part by weight of magnesium sulfate and 1.2 parts by weight of ferrous sulfate, carrying out steam sterilization on the first culture medium at 115 ℃ for 25min, transferring the first culture medium into a constant temperature cabinet at 23 ℃ for culturing for 6 days until mycelia grow fully in a culture container, and obtaining required protospecies mycelia;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn prepared in the step S2 onto a second culture medium in a polypropylene cultivation bag with the diameter of 6cm, wherein the second culture medium is prepared by mixing 15 parts of gypsum powder, 40 parts of corn straws, 8 parts of water and 8 parts of soybean meal, the water content of the second culture medium is 60%, and the second culture medium is subjected to steam sterilization at 115 ℃ for 20min and then subjected to cultivation operation until the cultivation bag is filled with poria cocos mycelium, the cultivation bag is tightened and placed in an aseptic environment, the cultivation bag is cultivated at a constant temperature of 12 ℃ for 8 days, then the cultivation bag is cultivated at a constant temperature of 25 ℃ for 15 days until the cultivation bag is filled with the poria cocos mycelium, and then cultivated hypha is obtained;
s4, wood guiding operation: peeling and air-drying pine tree branches with the diameter of 5cm and the length of 60cm, soaking the pine tree branches in saturated brown sugar water for one day and night, keeping 70% of water in the peeled and air-dried pine tree branches, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag of hyphae of a cultivar obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, and culturing and propagating at the temperature of 25 ℃ for 13 days;
s6, preparing a nutrition package: mixing pine sawdust, pine wood chips, pine hair, corn flour, wheat bran, brown sugar, gypsum, soybean meal and other raw materials in proportion, sterilizing at the high temperature of 121 ℃ for 4 hours, and cooling for later use;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the S5 step into the nutrition bag prepared in the S6 step under aseptic conditions, and culturing at 15-30 ℃ for 30-40 days;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken, inoculated on the cross section of the leading wood and wrapped by an agricultural film.
S10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, checking the growth condition of hyphae in each section for timely reseeding, if the poria cocos is exposed out of the ground, covering soil, weeding and killing insects in time, carrying out insecticidal treatment by using termite-killing medicines for killing insects, keeping the soil layer moist in the process of timely covering the soil, and paying attention to drainage in rainy seasons.
Example two: referring to fig. 1, the present invention discloses a planting method of poria cocos, comprising the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, taking out the poria cocos meat in the middle of the poria cocos blocks, inoculating the poria cocos blocks onto a first culture medium in a triangular flask, adding water into the first culture medium to mix until the water content of the first culture medium is 55%, performing steam sterilization at 120 ℃ for 25min, transferring the first culture medium into a constant temperature box at 25 ℃ to culture for 6 days until mycelia grow fully in the culture container, and obtaining required protospecies mycelia, wherein the first culture medium comprises 100 parts by weight of sweet potatoes, 35 parts by weight of glucose, 25 parts by weight of agar, 2 parts by weight of potassium dihydrogen phosphate, 0.8 part by weight of magnesium sulfate and 1.2 parts by weight of ferrous sulfate;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn prepared in the step S2 onto a second culture medium in a polypropylene cultivation bag with the diameter of 6cm, wherein the second culture medium is prepared by mixing 15 parts of gypsum powder, 40 parts of corn straws, 7 parts of water and 8 parts of soybean meal, the water content of the second culture medium is 70%, and the second culture medium is subjected to steam sterilization at 120 ℃ for 20min and then subjected to cultivation operation until the cultivation bag is filled with poria cocos mycelium, the cultivation bag is tightened and placed in an aseptic environment, the cultivation bag is cultivated at a constant temperature of 15 ℃ for 7 days, then cultivated at a constant temperature of 25 ℃ for 18 days until the cultivation bag is filled with the poria cocos mycelium, and then cultivated hypha is obtained;
s4, wood guiding operation: peeling and air-drying pine tree branches with the diameter of 4cm and the length of 70cm, soaking the pine tree branches in saturated brown sugar water for one day and night, keeping 70% of water in the peeled and air-dried pine tree branches, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag of hyphae of a cultivar obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, and culturing and propagating at the temperature of 25 ℃ for 13 days;
s6, preparing a nutrition package: mixing pine sawdust, pine wood chips, pine hair, corn flour, wheat bran, brown sugar, gypsum, soybean meal and other raw materials in proportion, sterilizing at the high temperature of 121 ℃ for 4 hours, and cooling for later use;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the S5 step into the nutrition bag prepared in the S6 step under aseptic conditions, and culturing at 15-30 ℃ for 30-40 days;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken, inoculated on the cross section of the leading wood and wrapped by an agricultural film.
S10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, checking the growth condition of hyphae in each section for timely reseeding, if the poria cocos is exposed out of the ground, covering soil, weeding and killing insects in time, carrying out insecticidal treatment by using termite-killing medicines for killing insects, keeping the soil layer moist in the process of timely covering the soil, and paying attention to drainage in rainy seasons.
Example three: referring to fig. 1, the present invention discloses a planting method of poria cocos, comprising the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, taking out the poria cocos meat in the middle of the poria cocos blocks, inoculating the poria cocos blocks onto a first culture medium in a triangular flask, wherein the first culture medium comprises 80 parts by weight of sweet potatoes, 35 parts by weight of glucose, 25 parts by weight of agar, 2 parts by weight of potassium dihydrogen phosphate, 0.7 part by weight of magnesium sulfate and 1.8 parts by weight of ferrous sulfate, adding water, mixing to enable the water content of the first culture medium to be 55%, performing steam sterilization on the first culture medium at 120 ℃ for 25min, transferring the first culture medium into a constant temperature box at 25 ℃ for culturing for 6 days until mycelia grow fully in a culture container, and obtaining required protospecies mycelia;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn prepared in the step S2 onto a second culture medium in a polypropylene cultivation bag with the diameter of 6cm, wherein the second culture medium is formed by mixing 10 parts of gypsum powder, 45 parts of corn straws, 8 parts of water and 7 parts of soybean meal, the water content of the second culture medium is 75%, and the second culture medium is subjected to steam sterilization at 120 ℃ for 25min and subjected to cultivation operation until the cultivation bag is filled with poria cocos mycelium, the cultivation bag is tightened and placed in an aseptic environment, the cultivation bag is cultivated at a constant temperature of 15 ℃ for 8 days, then at a constant temperature of 30 ℃ for 20 days, and the cultivation bag is filled with the poria cocos mycelium, so that cultivated hypha is obtained;
s4, wood guiding operation: peeling and air-drying pine tree branches with the diameter of 5cm and the length of 65cm, soaking the pine tree branches in saturated brown sugar water for one day and night, keeping 65% of moisture in the peeled and air-dried pine tree branches, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag of hyphae of a cultivar obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, and culturing and propagating at the temperature of 25 ℃ for 10 days;
s6, preparing a nutrition package: mixing pine sawdust, pine wood chips, pine hair, corn flour, wheat bran, brown sugar, gypsum, soybean meal and other raw materials in proportion, sterilizing at the high temperature of 121 ℃ for 4 hours, and cooling for later use;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the S5 step into the nutrition bag prepared in the S6 step under aseptic conditions, and culturing at 15-30 ℃ for 30-40 days;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken, inoculated on the cross section of the leading wood and wrapped by an agricultural film.
S10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, checking the growth condition of hyphae in each section for timely reseeding, if the poria cocos is exposed out of the ground, covering soil, weeding and killing insects in time, carrying out insecticidal treatment by using termite-killing medicines for killing insects, keeping the soil layer moist in the process of timely covering the soil, and paying attention to drainage in rainy seasons.
And (4) judging the standard: through comparison of the three embodiments, the best effect is the second embodiment, so that the second embodiment is selected as the best embodiment, and the specific change of the amount also belongs to the protection scope of the technical scheme.
The invention has the beneficial effects that: according to the planting method of the poria cocos, original strain hyphae and cultivated strain hyphae are cultured, and strains are transferred to pine branches for culture after being propagated into the hyphae, so that the nutrition of the pine branches is effectively utilized, the survival rate of the strains is high, the emergence rate and the emergence rate are high, the yield is high, selective material taking is also free from a pure natural environment, the cost and the labor amount are reduced, meanwhile, the insecticidal treatment is effectively carried out, and the survival rate of the cultivated poria cocos and the quality of the grown poria cocos are improved; the method is simple, has less investment and high yield, is suitable for planting in mountainous areas and plains, has better product quality, ensures that sclerotia is compact, the meat is compact, the epidermis is dark brown, the individual shape is round, and the meat is tender and white after peeling, and has better quality.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. The planting method of the poria cocos is characterized by comprising the following steps:
s1, selecting strains: selecting high-quality poria cocos blocks suitable for local temperature and climate, cleaning and disinfecting the surfaces of the selected poria cocos blocks for later use;
s2, cultivation of stock hyphae: taking out the poria cocos blocks selected in the step S1, inoculating the poria cocos flesh in the poria cocos blocks to a culture medium I in a triangular flask, transferring the poria cocos blocks to a thermostat with the temperature of 22-25 ℃ for culturing for 5-7 days until mycelium grows in a culture container, and obtaining required stock hypha;
s3, cultivation of hyphae of the cultivated species: inoculating the stock spawn hyphae prepared in the step S2 onto a culture medium II in a polypropylene cultivation bag with the diameter of 6cm, and performing cultivation operation until the cultivation bag is full of poria cocos hyphae, so as to obtain cultivation spawn hyphae;
s4, wood guiding operation: peeling pine tree branches with the diameter of 3-5cm and the length of 30-40cm, air-drying, soaking in saturated brown sugar water for a day and a night, and sterilizing for later use;
s5, preparation of cultivars: putting the peeled pine branches obtained in the step S4 into a culture bag of the hyphae of the cultivated species obtained in the step S3, extending the peeled pine branches to the outer side of the culture bag, fastening the opening of the culture bag, and culturing and propagating at the temperature of 15-30 ℃ for 10-15 days for later use;
s6, preparing a nutrition package: mixing pine sawdust, pine wood chips, pine hair, corn flour, wheat bran, brown sugar, gypsum, soybean meal and other raw materials in proportion, sterilizing at the high temperature of 121 ℃ for 4 hours, and cooling for later use;
s7, inoculating and culturing a nutrition package: inserting the strain bag prepared in the S5 step into the nutrition bag prepared in the S6 step under aseptic conditions, and culturing at 15-30 deg.C for 30-40 days.
S8, soil preparation and land selection: selecting a planting field with good drainage, excavating cultivation pits in the planting field in sandy soil with the soil mainly comprising yellow sandy soil and the pH value of 3-5, and spraying a sterilizing liquid to the cultivation pits to perform disinfection and insect killing treatment after excavation;
s9, grafting; when hypha begins to attach to the leading wood, 50 g of fresh poria cocos blocks are taken, inoculated on the cross section of the leading wood and wrapped by an agricultural film.
S10, planting: transplanting the nutrition bag and the strains cultured and propagated in the step S9 into sandy soil, and placing the nutrition bag below the soil;
s11, post management: 7-10 days after inoculation, the growth conditions of hyphae in each section are checked so as to supplement the seeds in time, if the poria cocos is exposed out of the ground, soil covering, weeding and insect killing are carried out in time, and drainage is carried out in rainy season.
2. The method for planting poria cocos as claimed in claim 1, wherein the first culture medium in step S2 comprises 80-120 parts by weight of sweet potato, 30-40 parts by weight of glucose, 20-25 parts by weight of agar, l-3 parts by weight of monopotassium phosphate, 0.3-1 part by weight of magnesium sulfate, and 0.5-2 parts by weight of ferrous sulfate, and water is added to the mixture to make the water content of the mixture reach 50-60%.
3. The method as claimed in claim 1, wherein the culture medium prepared in step S2 is steam sterilized at 125 ℃ for 20-30 min.
4. The method for planting tuckahoe according to claim 1, wherein the second culture medium in step S3 is prepared by mixing 10-18 parts of gypsum powder, 35-45 parts of corn straw, 6-10 parts of water and 5-10 parts of soybean meal to make the water content between 55-75%.
5. The method as claimed in claim 1, wherein the second culture medium prepared in step S3 is steam sterilized at 110-120 ℃ for 15-25 min.
6. The method for planting Poria cocos as claimed in claim 1, wherein in step S3, the bag is tied, placed in a sterile environment, and cultured at a constant temperature of 10-15 ℃ for 4-10 days, then at a constant temperature of 20-30 ℃ for 15-20 days, until the bag is filled with Poria cocos mycelia, to obtain the hypha of the culture.
7. The method for planting tuckahoe according to claim 1, wherein 65-70% of moisture is remained in the pine branches peeled and air-dried in the step S4.
8. The method for planting Poria cocos as claimed in claim 1, wherein in step S9, an opening is cut in the agricultural film, and the thickness of the casing over the tree section in step S11 is 1-5 cm.
9. The method for planting tuckahoe, according to claim 1, wherein the soil layer is kept moist during the timely covering of soil in step S11, and the termite-killing agent for killing insects in step S8 is used for killing insects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110764632.5A CN113383674A (en) | 2021-07-07 | 2021-07-07 | Planting method of poria cocos |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110764632.5A CN113383674A (en) | 2021-07-07 | 2021-07-07 | Planting method of poria cocos |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113383674A true CN113383674A (en) | 2021-09-14 |
Family
ID=77625352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110764632.5A Withdrawn CN113383674A (en) | 2021-07-07 | 2021-07-07 | Planting method of poria cocos |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113383674A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113812303A (en) * | 2021-10-21 | 2021-12-21 | 宁洱天瑞农业生物资源开发有限公司 | Culture medium for planting poria cocos |
| CN114051887A (en) * | 2021-11-19 | 2022-02-18 | 云茯苓普洱有限公司 | Indoor planting method of poria cocos |
| CN115500208A (en) * | 2022-11-08 | 2022-12-23 | 成都医学院 | Method for cultivating poria cocos sporocarp |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168611A (en) * | 2011-12-20 | 2013-06-26 | 罗振新 | Method of culturing poria cocos through bag materials |
| CN103548578A (en) * | 2013-11-18 | 2014-02-05 | 陈永林 | Planting method for Chinese medicinal plant tuckahoe |
| CN105917960A (en) * | 2016-04-21 | 2016-09-07 | 宁洱通达中药材种植专业合作社 | Planting method for poria cocos |
| CN105981590A (en) * | 2015-03-04 | 2016-10-05 | 杜方平 | Ecological poria cocos wild imitating tending technology and application |
| CN106386178A (en) * | 2016-10-24 | 2017-02-15 | 蔡凤文 | Technology for cultivating poria cocos in bag |
| WO2017034086A1 (en) * | 2015-08-25 | 2017-03-02 | 대한민국(농촌진흥청장) | Culture medium composition for cultivating poria cocos, and method for cultivating poria cocos using same |
| CN107371783A (en) * | 2016-05-14 | 2017-11-24 | 云楚彝药有限公司 | A kind of method of Bag Material plantation Poria cocos |
| CN108401788A (en) * | 2018-06-08 | 2018-08-17 | 广东省中药研究所 | A kind of cultural method of Poria cocos |
| CN108934761A (en) * | 2018-07-26 | 2018-12-07 | 崔巍 | A kind of cultivating and growing method of Poria cocos |
| CN111869505A (en) * | 2020-08-12 | 2020-11-03 | 湖南美园生物科技发展有限公司 | Pine tuckahoe planting method |
-
2021
- 2021-07-07 CN CN202110764632.5A patent/CN113383674A/en not_active Withdrawn
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168611A (en) * | 2011-12-20 | 2013-06-26 | 罗振新 | Method of culturing poria cocos through bag materials |
| CN103548578A (en) * | 2013-11-18 | 2014-02-05 | 陈永林 | Planting method for Chinese medicinal plant tuckahoe |
| CN105981590A (en) * | 2015-03-04 | 2016-10-05 | 杜方平 | Ecological poria cocos wild imitating tending technology and application |
| WO2017034086A1 (en) * | 2015-08-25 | 2017-03-02 | 대한민국(농촌진흥청장) | Culture medium composition for cultivating poria cocos, and method for cultivating poria cocos using same |
| CN105917960A (en) * | 2016-04-21 | 2016-09-07 | 宁洱通达中药材种植专业合作社 | Planting method for poria cocos |
| CN107371783A (en) * | 2016-05-14 | 2017-11-24 | 云楚彝药有限公司 | A kind of method of Bag Material plantation Poria cocos |
| CN106386178A (en) * | 2016-10-24 | 2017-02-15 | 蔡凤文 | Technology for cultivating poria cocos in bag |
| CN108401788A (en) * | 2018-06-08 | 2018-08-17 | 广东省中药研究所 | A kind of cultural method of Poria cocos |
| CN108934761A (en) * | 2018-07-26 | 2018-12-07 | 崔巍 | A kind of cultivating and growing method of Poria cocos |
| CN111869505A (en) * | 2020-08-12 | 2020-11-03 | 湖南美园生物科技发展有限公司 | Pine tuckahoe planting method |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113812303A (en) * | 2021-10-21 | 2021-12-21 | 宁洱天瑞农业生物资源开发有限公司 | Culture medium for planting poria cocos |
| CN113812303B (en) * | 2021-10-21 | 2023-01-31 | 宁洱天瑞农业生物资源开发有限公司 | Culture medium for planting poria cocos |
| CN114051887A (en) * | 2021-11-19 | 2022-02-18 | 云茯苓普洱有限公司 | Indoor planting method of poria cocos |
| CN115500208A (en) * | 2022-11-08 | 2022-12-23 | 成都医学院 | Method for cultivating poria cocos sporocarp |
| CN115500208B (en) * | 2022-11-08 | 2024-03-19 | 成都医学院 | Method for cultivating poria cocos fruiting bodies |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104041330B (en) | Ganoderma tsugae imitates wild juggle cultivation method | |
| CN108401788B (en) | Cultivation method of poria cocos | |
| CN105052669B (en) | Interplant the implantation methods of strawberry in vineyard | |
| CN113383674A (en) | Planting method of poria cocos | |
| CN106856978A (en) | Hickory chick artificial cultivation method | |
| CN105613047A (en) | Planting method of Letinus edodes | |
| CN107439217B (en) | Pollution-free cultivation method for fruit type sweet potatoes | |
| CN105981590A (en) | Ecological poria cocos wild imitating tending technology and application | |
| CN108812053A (en) | A kind of culture medium and cultural method of indoor growing selenium-rich Stropharia rugoso-annulata | |
| KR20170079322A (en) | Cultivation method of termite mushroom of using selenium | |
| CN106718735A (en) | A kind of method for planting dendrobium candidum in the tree | |
| CN107624489A (en) | A kind of implantation methods of selenium-rich lichee | |
| CN106717942A (en) | A kind of cultural method of the wild Huai Shan of High aititude | |
| CN104885745A (en) | Method for interplanting of platycodon grandiflorus and cowpea | |
| CN106508578A (en) | Cultivation method of honey pomelo | |
| CN107517812A (en) | A kind of implantation methods of ginkgo | |
| CN105010066A (en) | Interplanting method for plum trees and loquat trees | |
| CN107125017A (en) | A kind of breeding method of mushroom | |
| CN109122032A (en) | A kind of implantation methods of tea tree interplanting white beech mushroom | |
| CN107864810A (en) | The organic implantation methods of passion fruit | |
| CN107736177A (en) | Displant to fast-growing eucalyptus the cultural method of Moringa | |
| CN107371790A (en) | A kind of artificial method for planting of black fungus | |
| CN107455132A (en) | A kind of method of the nuisanceless blanching culture of blanched garlic leaves | |
| CN106688622A (en) | Culture medium formula of artificially cultivated armillaria mellea and cultivation method | |
| CN106748362A (en) | A kind of method for planting dendrobium candidum in the tree |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210914 |
|
| WW01 | Invention patent application withdrawn after publication |