CN105981590A

CN105981590A - Ecological poria cocos wild imitating tending technology and application

Info

Publication number: CN105981590A
Application number: CN201510095913.0A
Authority: CN
Inventors: 彭代银; 杜方平; 陈卫东; 俞年军; 贾步云
Original assignee: Anhui University of Traditional Chinese Medicine AHUTCM
Current assignee: Anhui University of Traditional Chinese Medicine AHUTCM
Priority date: 2015-03-04
Filing date: 2015-03-04
Publication date: 2016-10-05

Abstract

The invention relates to an ecological poria cocos wild imitating tending technology and application and belongs to the technical field of edible (medical) fungus cultivation and application. According to the technology, wild imitating tending cultivation of poria cocos is carried out with pine tree base roots under a wild pine tree forest, and tending cultivation is carried out with an optimized wild poria cocos strain; the cultivation steps mainly include strain production, sterile pretreatment on pine tree base roots, inoculation, tending management, harvesting and processing, and deep processing for application. According to the technology, by carrying out wild imitating tending cultivation of poria cocos with pine tree base roots, the ecological environment of a pine tree area is protected, medical poria cocos with the quality equivalent to that of wild poria cocos can be produced, the quality of poria cocos medical materials is improved while financial resources and material resources needed for planting poria cocos are reduced, and the ecological sustainable development of the poria cocos industry is promoted.

Description

A kind of ecology Poria imitates natural cultivation technology and application

Technical field

The invention belongs to eat (medicine) and use bacteria cultivation technical field, be more particularly to a kind of ecology Poria and imitate natural cultivation technology and application.

Background technology

Poria is the dry sclerotia of On Polyporaceae Poria Poria cocos (Schw.) Wolf, has the effects such as promoting diuresis to eliminate damp pathogen, invigorating the spleen and regulating the stomach, mind tranquilizing and the heart calming.As the conventional Chinese medicine material of China, Poria major production areas is Dabie Mountain and the Yunnan-guizhou Area of boundary, E Yu Anhui, especially maximum with Anhui yield, for one of big famous medicine in Anhui four, claims " peace Siberian cocklebur ", for Poria emphasis production base, the whole nation.The ground such as main producing region Anhui Province Jinzhai County, Yuexi County, Hinterland of The Dabie Mountains at this region, height above sea level, day and night the temperature difference is big, rainfall is abundant, the plantation of the mushroom Chinese crude drugs such as the particularly suitable Poria of natural environment and cultivation, and the cultivation that produces of Poria has long history, more ripe technology and experience.But, the cultivation of current Poria still in the traditional segment wood cultivated mode followed, has been cut down substantial amounts of pinaster resource, has been caused the destruction of ecological environment.Thus, the invention provides a kind of technology using the ecology carrying out Poria of pinaster base root under pine forest to imitate natural cultivation cultivation, not only protect pinaster resource, cultivate the high-quality Poria of growth under wild environment simultaneously.

Summary of the invention

The invention provides a kind of high yield, the imitative natural cultivation cultivation of high-quality Poria and the method for application thereof.

The invention provides a kind of ecology Poria and imitate natural cultivation technology and application, specifically: select pinaster base root under the preferred wild wild pine forests of Poria strain to carry out imitative natural cultivation cultivation；Cultivation step specifically includes that the strain aseptic pretreatment → inoculation of production → pinaster base root → imitative natural cultivation cultivation management → pick and process → deep processing application.

The concrete grammar that described raw strain produces includes: (1) Poria mother plants production: utilize preferred wild Poria sample to separate strain on PDA slant medium, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 150 ~ 300g(removes the peel), sucrose 1 ~ 50g, agar 1 ~ 50g, carbamide 1 ~ 10g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 20 ~ 30 DEG C treats that mycelia is covered with inclined-plane in 1 ~ 10 day, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 1 ~ 60%, pine sawdust 1 ~ 40%, wheat bran (or wheat bran) 1 ~ 40%, sucrose 1 ~ 10%, Gypsum Fibrosum powder 0.5 ~ 2%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 1 ~ 80%, pine sawdust 1 ~ 40%, wheat bran 1 ~ 40%, sucrose 1 ~ 10%, Gypsum Fibrosum powder 0.5 ~ 2%, carbamide 0.1 ~ 1%, calcium superphosphate 0.1 ~ 2%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 10 ~ 60 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

The described pinaster aseptic preprocess method of base root includes: when autumn, winter cut down pinaster, the stub that selection diameter is more than 12 centimetres, around ground weeds and shrub are cut down, deep-cut 20～60cm, stub and root is allowed to be exposed to outside soil, then shovel skin 1～6 on stub top respectively, stay 1 ~ 6 treaty 1 ~ 8cm width not shovel the muscle (being also lead-in wire) of skin.Thick tree root under stub also can be used to plant Poria, every tree root shovel skin 3, stays 3 lead-in wires.Tree base root is made fully to be exposed to the sun to parching.After Gan, tree base root is built by available grass, prevents rainfall from drenching and microbiological contamination.

Described inoculation method particularly as follows: select March ~ May, fine day by the cultigen being successful cultivation band tear an osculum, be close to the good pinaster base root of pretreatment prune root bark section on, earthing 5 ~ 20cm.

Described imitative natural cultivation management method is particularly as follows: people and animals to be forbidden trample on.Mycelial growth situation is checked after 7 ~ 10 days.As found, inoculation loses, and should mend cultivation immediately.Note watering soil moisture conservation simultaneously, the cloudy day timely draining of gas.Root out immediately if any weeds.After ridging Poria forms sclerotium (knot Siberian cocklebur), Siberian cocklebur body constantly increases or exposes soil face because heavy rain washes away topsoil, makes Poria stop growing.Therefore want duty to check, discovery table soil cracking is opened or Siberian cocklebur body exposes and to earth up in time.After inoculation then 7～JIUYUE ground temperature high, Coptotermes formosanus Shtrari. breeding is fast.Prevention and controls: find in time, pours water ant cave with boiled water, and adds a cover sand, deinsectization source of going out.

Described harvesting and processing method is particularly as follows: Poria of gathering general the most after inoculation 8～ripe in 10 months, and a feature of ripe Poria is outer belt yellowish-brown；Another feature is that the section wood of long sclerotium becomes loose in sepia, broken once pinching, and represents that nutriment is the most most, should gather immediately.Indoor lee stacked by Poria of gathering, cover tightly with Caulis et Folium Oryzae or gunnysack and be allowed to diaphoresis, separate out moisture, spread after drying heap lid repeatedly again out, to epidermis shrinkage brown time peel appearance casting skin (Cortex Sclerotii Poriae) with cutter after, select fine day to be cut into block sheet successively, the white block that will cut out, red piece respectively stand bamboo mat or bamboo sieve on dry.Also can directly peel fresh Poria crust rearmounted food steamer Steam by water bath off and parch the heart, taking-up scraper presses above-mentioned specification dice, puts and shines under sunlight to sufficient dry.

Described deep processing application process specifically includes: Poria powder health food development, the exploitation of high-quality Poria decoction pieces and the exploitation of Poria effective component extracts.

Optimize further:

A kind of ecology Poria imitates natural cultivation technology and application, specifically: select pinaster base root under the preferred wild wild pine forests of Poria strain to carry out imitative natural cultivation cultivation；Cultivation step specifically includes that the strain aseptic pretreatment → inoculation of production → pinaster base root → imitative natural cultivation cultivation management → pick and process → deep processing application.

The concrete grammar that described raw strain produces includes: (1) Poria mother plants production: utilize preferred wild Poria sample to separate strain on PDA slant medium, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 150 ~ 200g(removes the peel), sucrose 1 ~ 30g, agar 1 ~ 20g, carbamide 1 ~ 5g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 20 ~ 30 DEG C treats that mycelia is covered with inclined-plane in 5 ~ 7 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 40 ~ 60%, pine sawdust 20 ~ 40%, wheat bran (or wheat bran) 20 ~ 40%, sucrose 1 ~ 5%, Gypsum Fibrosum powder 1 ~ 2%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 40 ~ 80%, pine sawdust 20 ~ 40%, wheat bran 20 ~ 40%, sucrose 1 ~ 5%, Gypsum Fibrosum powder 1 ~ 2%, carbamide 0.1 ~ 0.5%, calcium superphosphate 0.1 ~ 1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 30 ~ 60 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

The described pinaster aseptic preprocess method of base root includes: when autumn, winter cut down pinaster, the stub that selection diameter is more than 12 centimetres, around ground weeds and shrub are cut down, deep-cut 20～40cm, stub and root is allowed to be exposed to outside soil, then shovel skin 3～6 on stub top respectively, stay 3 ~ 6 treaty 5 ~ 8cm width not shovel the muscle (being also lead-in wire) of skin.Thick tree root under stub also can be used to plant Poria, every tree root shovel skin 3, stays 3 lead-in wires.Tree base root is made fully to be exposed to the sun to parching.After Gan, tree base root is built by available grass, prevents rainfall from drenching and microbiological contamination.

Described inoculation method particularly as follows: select March ~ April, fine day by the cultigen being successful cultivation band tear an osculum, be close to the good pinaster base root of pretreatment prune root bark section on, earthing 10 ~ 20cm.

It is an advantage of the current invention that:

1) the Poria active constituent content that the present invention produces is high, and treatment and health-care effect are more preferably

2) present invention uses the mode that imitative natural cultivation is cultivated, and the Poria quality produced, closer to wild Poria, maintains the advantage of the wild Poria in Genuine producing area.

3) present invention utilizes forestry waste pinaster base root to cultivate, and protects the pinaster forest resourceies in plantation area, the improvement of beneficially ecological environment and the sustainable development of Poria plantation industry.

Detailed description of the invention

Once further illustrate the present invention by detailed description of the invention, be construed as limiting the invention the most in any form.

Embodiment 1

Natural cultivation technology imitated by a kind of ecology Poria, and concrete operations are as follows:

1, strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachyman content (4.186%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 150g(removes the peel), sucrose 10g, agar 10g, carbamide 1g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 30 DEG C treats that mycelia is covered with inclined-plane in 7 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 50%, pine sawdust 20%, wheat bran (or wheat bran) 10%, sucrose 1%, Gypsum Fibrosum powder 1%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 60%, pine sawdust 20%, wheat bran 15%, sucrose 1%, Gypsum Fibrosum powder 2%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

2, the aseptic pretreatment of pinaster base root: when autumn, winter cut down pinaster, the stub that selection diameter is more than 12 centimetres, around ground weeds and shrub are cut down, deep-cut 20cm, stub and root is allowed to be exposed to outside soil, then shovel skin 5 on stub top respectively, stay 5 treaty 5 ~ 8cm width not shovel the muscle (being also lead-in wire) of skin.Thick tree root under stub also can be used to plant Poria, every tree root shovel skin 3, stays 3 lead-in wires.Tree base root is made fully to be exposed to the sun to parching.After Gan, tree base root is built by available grass, prevents rainfall from drenching and microbiological contamination.

3, inoculation: select early March, fine day by the cultigen being successful cultivation band tear an osculum, be close to the good pinaster base root of pretreatment prune root bark section on, earthing 20cm.

4, imitative natural cultivation management: people and animals to be forbidden trample on.Mycelial growth situation is checked after 7 days.As found, inoculation loses, and should mend cultivation immediately.Note watering soil moisture conservation simultaneously, the cloudy day timely draining of gas.Root out immediately if any weeds.After ridging Poria forms sclerotium (knot Siberian cocklebur), Siberian cocklebur body constantly increases or exposes soil face because heavy rain washes away topsoil, makes Poria stop growing.Therefore want duty to check, discovery table soil cracking is opened or Siberian cocklebur body exposes and to earth up in time.After inoculation then 7～JIUYUE ground temperature high, Coptotermes formosanus Shtrari. breeding is fast.Prevention and controls: find in time, pours water ant cave with boiled water, and adds a cover sand, deinsectization source of going out.

5, pick and process: Poria of gathering general the most after inoculation 8～ripe in 10 months, a feature of ripe Poria is outer belt yellowish-brown；Another feature is that the section wood of long sclerotium becomes loose in sepia, broken once pinching, and represents that nutriment is the most most, should gather immediately.Indoor lee stacked by Poria of gathering, cover tightly with Caulis et Folium Oryzae or gunnysack and be allowed to diaphoresis, separate out moisture, spread after drying heap lid repeatedly again out, to epidermis shrinkage brown time peel appearance casting skin (Cortex Sclerotii Poriae) with cutter after, select fine day to be cut into block sheet successively, the white block that will cut out, red piece respectively stand bamboo mat or bamboo sieve on dry.Also can directly peel fresh Poria crust rearmounted food steamer Steam by water bath off and parch the heart, taking-up scraper presses above-mentioned specification dice, puts and shines under sunlight to sufficient dry.

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 1).

Pachyman and pachymic acid assay result in table 1 Poria

Project	Content (%)
		Pachyman	4.302
Pachymic acid	0.934

Embodiment 2

1, strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachyman content (4.186%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 180g(removes the peel), sucrose 15g, agar 12g, carbamide 2g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 30 DEG C treats that mycelia is covered with inclined-plane in 7 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 40%, pine sawdust 30%, wheat bran (or wheat bran) 15%, sucrose 2%, Gypsum Fibrosum powder 2%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 50%, pine sawdust 30%, wheat bran 15%, sucrose 1%, Gypsum Fibrosum powder 2%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 2).

Pachyman and pachymic acid assay result in table 2 Poria

Project	Content (%)
		Pachyman	4.419
Pachymic acid	0.871

Embodiment 3

1, strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachyman content (4.186%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 200g(removes the peel), sucrose 20g, agar 18g, carbamide 2g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 30 DEG C treats that mycelia is covered with inclined-plane in 7 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 35%, pine sawdust 35%, wheat bran (or wheat bran) 15%, sucrose 2%, Gypsum Fibrosum powder 2%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 40%, pine sawdust 40%, wheat bran 15%, sucrose 1%, Gypsum Fibrosum powder 2%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 3).

Pachyman and pachymic acid assay result in table 3 Poria

Project	Content (%)
		Pachyman	4.322
Pachymic acid	0.843

Embodiment 4

1, strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachyman content (4.186%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 200g(removes the peel), sucrose 10g, agar 10g, carbamide 1g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 25 DEG C treats that mycelia is covered with inclined-plane in 5 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 35%, pine sawdust 35%, wheat bran (or wheat bran) 10%, sucrose 1%, Gypsum Fibrosum powder 1%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 40%, pine sawdust 40%, wheat bran 10%, sucrose 1%, Gypsum Fibrosum powder 1%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 4).

Pachyman and pachymic acid assay result in table 4 Poria

Project	Content (%)
		Pachyman	4.319
Pachymic acid	0.851

Embodiment 5

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 5).

Pachyman and pachymic acid assay result in table 5 Poria

Project	Content (%)
		Pachyman	4.213
Pachymic acid	0.802

Embodiment 6

1, strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachymic acid content (0.818%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 180g(removes the peel), sucrose 15g, agar 12g, carbamide 2g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 30 DEG C treats that mycelia is covered with inclined-plane in 7 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 40%, pine sawdust 30%, wheat bran (or wheat bran) 15%, sucrose 2%, Gypsum Fibrosum powder 2%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 50%, pine sawdust 30%, wheat bran 15%, sucrose 1%, Gypsum Fibrosum powder 2%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

5, pick and process: Poria of gathering general the most after inoculation 8～ripe in 10 months, a feature of ripe Poria is outer belt yellowish-brown；Another feature is that the section wood of long sclerotium becomes loose in sepia, broken once pinching, and represents that nutriment is the most most, should gather immediately.Indoor lee stacked by Poria of gathering, cover tightly with Caulis et Folium Oryzae or gunnysack and be allowed to diaphoresis, separate out moisture, spread after drying heap lid repeatedly again out, to epidermis shrinkage brown time peel appearance casting skin (Cortex Sclerotii Poriae) with cutter after, select fine day to be cut into block sheet successively, the white block that will cut out, red piece respectively stand bamboo mat or bamboo sieve on dry.Also can directly peel fresh Poria crust rearmounted food steamer Steam by water bath off and parch the heart, taking-up scraper presses above-mentioned specification dice, puts and shines under sunlight to sufficient dry

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 6).

Pachyman and pachymic acid assay result in table 6 Poria

Project	Content (%)
		Pachyman	3.335
Pachymic acid	0.971

Embodiment 7

1, strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachymic acid content (0.818%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 200g(removes the peel), sucrose 20g, agar 18g, carbamide 2g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 30 DEG C treats that mycelia is covered with inclined-plane in 7 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 35%, pine sawdust 35%, wheat bran (or wheat bran) 15%, sucrose 2%, Gypsum Fibrosum powder 2%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 40%, pine sawdust 40%, wheat bran 15%, sucrose 1%, Gypsum Fibrosum powder 2%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 7).

Pachyman and pachymic acid assay result in table 7 Poria

Project	Content (%)
		Pachyman	3.329
Pachymic acid	0.973

Embodiment 8

1, strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachymic acid content (0.818%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 200g(removes the peel), sucrose 10g, agar 10g, carbamide 1g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 25 DEG C treats that mycelia is covered with inclined-plane in 5 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 35%, pine sawdust 35%, wheat bran (or wheat bran) 10%, sucrose 1%, Gypsum Fibrosum powder 1%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 40%, pine sawdust 40%, wheat bran 10%, sucrose 1%, Gypsum Fibrosum powder 1%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 8).

Pachyman and pachymic acid assay result in table 8 Poria

Project	Content (%)
		Pachyman	3.459
Pachymic acid	0.838

Embodiment 9

1, strain produces: (1) strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachymic acid content (0.818%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 180g(removes the peel), sucrose 20g, agar 10g, carbamide 1g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 30 DEG C treats that mycelia is covered with inclined-plane in 6 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 35%, pine sawdust 35%, wheat bran (or wheat bran) 10%, sucrose 1%, Gypsum Fibrosum powder 1%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 45%, pine sawdust 35%, wheat bran 10%, sucrose 1%, Gypsum Fibrosum powder 1%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 9).

Pachyman and pachymic acid assay result in table 9 Poria

Project	Content (%)
		Pachyman	3.435
Pachymic acid	0.971

Embodiment 10

1, strain produces: (1) Poria mother plants production: in the wild Poria specimen utilizing preferred Da Bie Mountain area to gather active component pachymic acid content (0.818%) higher Poria sample on PDA slant medium, separate strain, as one-level mother's kind.Female concrete manufacture method of planting is: culture medium prescription: Rhizoma Solani tuber osi 190g(removes the peel), sucrose 10g, agar 15g, carbamide 1g, water 1000mL.Above-mentioned formula is made test tube slant culture medium according to a conventional method.And aseptically, use tissue isolation.In poria cocos sclerotium one piece of white Poria meat is accessed medium slant.The indoor cultivation moving into 30 DEG C treats that mycelia is covered with inclined-plane in 6 days, one-level mother's kind.(2) Poria seed production: using with Masson Pine block, pine sawdust is the culture medium of primary raw material, accesses one-level mother kind and produces.The concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 40%, pine sawdust 3%, wheat bran (or wheat bran) 10%, sucrose 1%, Gypsum Fibrosum powder 1%, water is appropriate.After compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters.Loading amount is 4/5ths of bottle.Slightly it is compacted, makes a call to an air-vent in central authorities.Sterilization treatment is carried out after sealing with tampon.It is cooled to when 20 ~ 30 DEG C aseptically one-level mother to be planted on access culture in glassware base.After sealing, cultivate, treat that mycelia is covered with, be original seed.(3) production of tuckahoe cultivated species: use the culture medium with Masson Pine block, pine sawdust etc. as primary raw material, accesses cultigen and produces.Concrete mode is: culture material formula: Masson Pine block 50%, pine sawdust 30%, wheat bran 10%, sucrose 1%, Gypsum Fibrosum powder 1%, carbamide 0.1%, calcium superphosphate 0.1%, water is appropriate.Above-mentioned dispensing being mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion.Then it is distributed in 500 milliliters of culture bag, sealing, carries out sterilization treatment.When culture medium temperature is down to 35 DEG C, just can aseptically original seed be tapped in dispensing.After inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

6, pachyman and pachymic acid assay: use phend-sulphuric acid to measure the content of pachyman, uses HPLC method to measure the content of pachymic acid, and result is following (table 10).

Table 10 Pachyman and pachymic acid assay result in Poria

Project	Content (%)
		Pachyman	4.02
Pachymic acid	0.975

Claims

1. an ecology Poria imitates natural cultivation technology and application, it is characterised in that: use pinaster base root under the preferred wild wild pine forests of Poria strain to carry out imitative natural cultivation cultivation；Cultivation step specifically includes that the strain aseptic pretreatment → inoculation of production → pinaster base root → imitative natural cultivation cultivation management → pick and process → deep processing application.

2. imitate natural cultivation technology and application according to ecology Poria described in claim 1, it is characterized in that the concrete grammar that described strain produces includes: (1) Poria mother plants production: utilize preferred wild Poria sample to separate strain on PDA slant medium, as one-level mother's kind, the concrete manufacture method of female kind is: culture medium prescription: Rhizoma Solani tuber osi 150 ~ 300g(removes the peel), sucrose 1 ~ 50g, agar 1 ~ 50g, carbamide 1 ~ 10g, water 1000mL, above-mentioned formula is made test tube slant culture medium according to a conventional method, and aseptically, use tissue isolation, in poria cocos sclerotium one piece of white Poria meat is accessed medium slant, the indoor cultivation moving into 20 ~ 30 DEG C treats that mycelia is covered with inclined-plane in 1 ~ 10 day, one-level mother's kind；(2) Poria seed production: use with Masson Pine block, pine sawdust is the culture medium of primary raw material, access one-level mother kind to produce, the concrete manufacture method of original seed is: Medium for original variety is: Masson Pine block 1 ~ 60%, pine sawdust 1 ~ 40%, wheat bran (or wheat bran) 1 ~ 40%, sucrose 1 ~ 10%, Gypsum Fibrosum powder 0.5 ~ 2%, water is appropriate, after compost in formula is mixed all, it is distributed in the wealthy mouth bottle of 500 milliliters, loading amount is 4/5ths of bottle, slightly it is compacted, an air-vent is made a call in central authorities, sterilization treatment is carried out after sealing with tampon, can be aseptically when being cooled to 20 ~ 30 DEG C, one-level mother is planted on access culture in glassware base, after sealing, cultivate, treat that mycelia is covered with, it is original seed；(3) production of tuckahoe cultivated species: use with Masson Pine block, the culture medium that pine sawdust etc. are primary raw material, access cultigen to produce, concrete mode is: culture material formula: Masson Pine block 1 ~ 80%, pine sawdust 1 ~ 40%, wheat bran 1 ~ 40%, sucrose 1 ~ 10%, Gypsum Fibrosum powder 0.5 ~ 2%, carbamide 0.1 ~ 1%, calcium superphosphate 0.1 ~ 2%, water is appropriate, above-mentioned dispensing is mixed uniformly, temperature is for being held by hand expecting that webs have water not drip and be as the criterion, then it is distributed in 500 milliliters of culture bag, sealing, carry out sterilization treatment, when culture medium temperature is down to 10 ~ 60 DEG C, just can be aseptically, original seed is tapped in dispensing, after inoculation, culture bag is moved to culturing room cultivate to mycelia cover with full bag time, obtain cultigen.

Natural cultivation technology imitated by ecology Poria the most according to claim 1, it is characterized in that the described pinaster aseptic preprocess method of base root includes: the autumn, when winter cuts down pinaster, the stub that selection diameter is more than 12 centimetres, around ground weeds and shrub are cut down, deep-cut 20～60cm, stub and root is allowed to be exposed to outside soil, then skin 1～6 are shoveled respectively on stub top, 1 ~ 6 treaty 1 ~ 8cm width is stayed not shovel the muscle (being also lead-in wire) of skin, thick tree root under stub also can be used to plant Poria, every tree root shovel skin 3, stay 3 lead-in wires, tree base root is made fully to be exposed to the sun to parching, after Gan, tree base root is built by available grass, prevent rainfall from drenching and microbiological contamination.

Natural cultivation technology imitated by ecology Poria the most according to claim 1, it is characterized in that described inoculation method particularly as follows: select March ~ May, the cultigen being successful cultivation band is torn an osculum by fine day, be close to the good pinaster base root of pretreatment prune root bark section on, earthing 5 ~ 20cm.

Natural cultivation technology imitated by ecology Poria the most according to claim 1, it is characterized in that described imitative natural cultivation management method is particularly as follows: people and animals to be forbidden trample on, mycelial growth situation is checked after 7 ~ 10 days, as found, inoculation loses, cultivation should be mended immediately, note watering soil moisture conservation simultaneously, the cloudy day timely draining of gas, root out immediately if any weeds, after ridging Poria forms sclerotium (knot Siberian cocklebur), Siberian cocklebur body constantly increases or exposes soil face because heavy rain washes away topsoil, Poria is made to stop growing, therefore want duty to check, discovery table soil cracking is opened or Siberian cocklebur body exposes and to earth up in time, after inoculation then 7～JIUYUE ground temperature high, Coptotermes formosanus Shtrari. breeding is fast, prevention and controls: find in time, pour water ant cave with boiled water, and add a cover sand, go out deinsectization source.

Natural cultivation technology imitated by ecology Poria the most according to claim 1, it is characterised in that described harvesting and processing method is particularly as follows: Poria of gathering general the most after inoculation 8～ripe in 10 months, and a feature of ripe Poria is outer belt yellowish-brown；Another feature is that the section wood of long sclerotium becomes loose in sepia, broken once pinching, represent that nutriment is the most most, should gather immediately, indoor lee stacked by Poria of gathering, cover tightly with Caulis et Folium Oryzae or gunnysack and be allowed to diaphoresis, separate out moisture, spread after drying heap lid repeatedly again out, to epidermis shrinkage brown time peel appearance casting skin (Cortex Sclerotii Poriae) with cutter after, fine day is selected to be cut into block sheet successively, the white block that will cut out, red piece respectively stand bamboo mat or bamboo sieve on dry, also can directly peel fresh Poria crust rearmounted food steamer Steam by water bath off and parch the heart, taking-up scraper presses above-mentioned specification dice, put and shine under sunlight to sufficient dry.

Natural cultivation technology imitated by ecology Poria the most according to claim 1, it is characterised in that described deep processing application process specifically includes: Poria powder health food development, the exploitation of high-quality Poria decoction pieces and the exploitation of Poria effective component extracts.