CN103548578A - Planting method for Chinese medicinal plant tuckahoe - Google Patents
Planting method for Chinese medicinal plant tuckahoe Download PDFInfo
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Abstract
The invention relates to a planting method for a Chinese medicinal plant tuckahoe. The planting method comprises the following steps: selecting strain seeds, culturing the strain seeds, adjusting the pH value of soil, disinsecting, preparing wood, inoculating, carrying out field management, preventing and eliminating plant diseases and insect pests, harvesting and processing, so as to obtain high-quality tuckahoe meeting the requirements of the market. The tuckahoe obtained according to the planting method is purely natural, and high in quality and yield.
Description
Technical field
The present invention relates to the implantation methods of a kind of implantation methods of traditional Chinese medicine, particularly Poria cocos.
Background technology
Poria cocos, is the dry sclerotia of On Polyporaceae Poria cocos Poria cocos (Schw.) Wolf, and another name cloud Siberian cocklebur, loose Poria cocos, be the fungi colonizing on pine tree.Medicinal part is dry sclerotia body.Document is the earliest recorded and is come from < < Sheng Nong's herbal classic > >, be commonly called as Yun Ling, Song Ling, Fu spirit, for colonizing in the fungi plant on pine roots, shape is as sweet potato, crust pitchy, the inside white or pink.Its eobiont is the dry sclerotia of On Polyporaceae Poria cocos, and multiparasitism is in the root of masson pine or Japanese red pine.Originate in the ground such as Yunnan, Anhui, Hubei, Henan, Sichuan.Ancients claim that Poria cocos is " god medicine " at 4 o'clock, because its effect is very extensive, regardless of the four seasons, by it and various compatibility of drugs, no matter cold, temperature, wind, all diseases that wets can be brought into play its unique effects.Poria cocos taste is sweet, light, property is flat, be used as medicine there is clearing damp and promoting diuresis, the function of beneficial spleen and stomach, antitoxic heart-soothing and sedative.Modern medicine study: Poria cocos energy enhanced machine body immunity function, pachymaran has significantly antitumor and protect the liver dirty effect.
Pharmacological effect: traditional effect, clearing damp and promoting diuresis, strengthening the spleen and reducing phlegm, antitoxic heart-soothing and sedative, relieves internal heat anticancer.The property of medicine is gentle, dampness removing and do not hinder healthy tendency.Take in right amount the good merchantable brand of taking good care of that can be used as spring and summer wet season, can control that difficult urination, oedema turgor, phlegm and retained fluid are coughed contrary, contrary, the vomiting during early pregnancy of vomitting, had loose bowels, seminal emission, stranguria with turbid discharge, palpitation with fear, the disease such as forgetful.Contained pachymic acid has and strengthens immunity, antitumor and calm, hypoglycemic etc. effect.The gastrointestinal smooth muscle that can relax, the effect such as gastric acid secretion inhibiting, prevents necrosis of liver cells, antibiotic.The effect of latest find, the main component in Poria cocos is pachyman, content is very high.Various bacteria is had to inhibitory action; Can fall hydrochloric acid in gastric juice, digestive tract ulcer is had to preventive effect; Hepatic injury is had to significant protective effect; There is antineoplastic action; Can many-sided immunologic function be regulated; Can make due to chemotherapy leucocyte reduce accelerates; And there is a calm effect.
Poria cocos multiparasitism is upper in masson pine or Duan Mu, and it grows and can be divided into two stages: i.e. mycelia (white filiform) stage and sclerotium stage.Vegetative stage, is mainly that mycelia absorbs moisture and nutrition from wood surface, and secretase decomposes and transforms the organic matter (cellulose) in timber simultaneously, makes mycelia spread over vigorous growth in timber.Second stage is that mycelia is coalescent agglomerating to the middle and later periods, forms gradually sclerotium (also claiming knot etc.).Knot Siberian cocklebur size has substantial connection with the envirment factors such as quality, nutritional condition and temperature, humidity of bacterial classification.The bacterial classification of different cultivars, the time length of knot Siberian cocklebur is also different, and some kinds start to tie Siberian cocklebur for 3～4 months after planting, and some is slower, needs 6～7 months.Early maturing variety can be gathered in the crops after planting for 9～10 months, and late-maturing kind needs 12～14 months.
The place that Poria cocos happiness is warm, be dried, face south, avoids north wind and blows and scrape, and distributes the widest with height above sea level in the pine forest of 700 meters of left and right, and temperature is advisable with 10～35.The artificial planting of Poria cocos need to be used pine tree, plant every mu of Poria cocos pine tree consumption at 6-10 ton left and right timber, every mu of Poria cocos bacterial classification needs 1800-2000 bag, the general about 1000-2000 kilogram of per mu yield, but at present because not science is planted in various places, pine tree large usage quantity, yield poorly, so cost is higher, how in the situation that reducing pine tree consumption of wood, the output and the quality that improve Poria cocos, also have a lot of people to make great efforts to carry out exploratory development at present.
Summary of the invention
The implantation methods that the object of this invention is to provide a kind of Poria cocos, in order to produce at the less pine tree timber of use, output is high, the measured Poria cocos of matter, meets the needs on market.
The present invention is achieved in that
The implantation methods of Poria cocos, comprise bacterial classification seed selection, bacterial classification cultivate, expand cultivate, soil is got the raw materials ready with acid-base value adjustment, desinsection, wood wholely, inoculation, field management, damage by disease and insect are prevented and kill off, the processing of gathering, obtains the process of high-quality Poria cocos,
Described bacterial classification seed selection is to choose single heavy more than 2.5 kilograms large Siberian cocklebur from the Poria cocos of the firm results of stable high yield, the special fine quality that adapts to local temperature, weather that adopts; Thalline is subsphaeroidal, and crust is thinner, light yellow or brownish red.Grow vigorous, appearance has obvious crackle.Inner Siberian cocklebur meat is white, and juice is more-and Poria cocos smell is strong, crust damages without insect bite, and without the difference of rotting, the kind Siberian cocklebur of collection is buried with wet lapped, with anti-drying.
It is the Poria cocos meat by the middle of getting after the above-mentioned kind Siberian cocklebur surface clean of choosing and sterilization that described bacterial classification is cultivated, and on the medium of access test tube slant or triangular flask, moves in 22～25 ℃ of insulating boxs and cultivates 5-7 days, obtains bacterial classification; Miscellaneous bacteria is wherein scalded miscellaneous bacteria dead or digs out processing with scorching hot inoculation shovel.Specifically the above-mentioned kind Siberian cocklebur of choosing is rinsed extremely without silt with clear water, with gauze, dry, then at the indoor 50-70% alcohol of inoculation, rinse whole bacterium ball, carry out surface sterilization, then use aseptic water washing 2-4 time, after kind of Siberian cocklebur surface dry, with aseptic cutter, be cut into two halves, the whiter Siberian cocklebur meat of picking soya bean size, on the medium of access test tube slant or triangular flask, labelled after inoculation, indicate kind of Siberian cocklebur source and inoculation time etc.Move in 22～25 ℃ of insulating boxs and cultivate, 5-7 days, obtains bacterial classification.
Potato agar medium used herein, fills a prescription as 200 grams, potato or Ipomoea batatas, 30 grams of glucose, 20 grams, agar, potassium dihydrogen phosphate l gram, 0.3 gram, magnesium sulfate, 0.5 gram, ferrous sulfate, 1000 milliliters, water; Medium needs sterilizing after carrying out.
It is pine to be cut into the fragment of 0.5～3 centimetre of size that described expansion is cultivated, 110～125 ℃ of steam sterilizings, 20～30 minutes, expand the access of Poria cocos mycelia in medium and fragment and cultivate, in the thermostatic chamber of 20～28 ℃, cultivate 5-10 days, mycelia is covered with media surface; After 5-7 days, the mycelia that bacterial classification is cultivated is covered with media surface and can expand cultivation, pine is cut into the fragment of 0.5～3 centimetre of size, 110～125 ℃ of steam sterilizings, 20～30 minutes, mix evenly with medium, be placed in flat board upper, the access of Poria cocos mycelia is expanded to medium culture.
Described medium is corn flour 30-50 weight portion, wheat bran 15～20 weight portions, manioc waste 20-40 weight portion, waste molasses of sugar plant 10-20 weight portion, potassium dihydrogen phosphate 5-18 weight portion, magnesium sulfate 1-3 weight portion, ferrous sulfate 1-3 weight portion, pine sawdust 150-200 weight portion, adds water mixing and makes its water content 60-90%, 110～125 ℃ of steam sterilizings, 20～30 minutes.
Wholelyly and be to select well-drained hillside, soil is take yellow ground soil as main for described soil; Or yellow clay is added to 1/3～2/3 fine sand mixing.
Acid-base value is adjusted, and Poria cocos is liked faintly acid soil, and pH value is 4.5 to 6.5; So will be according to the soil property situation of planting site,, alkaline Organic Manure on Soil acid-base value acid with lime, aluminum sulfate, ferrous sulfate or band adjusted, if below 4.5, can suitably use a small amount of lime, regulate pH to reduce some heavy metal element harm.And during the too high soil of alkalescence, can add a small amount of aluminum sulfate, ferrous sulfate, humic acid fertilizer etc., soil is increased acid.Content of beary metal must strictly be controlled, and also requires away from city and industrial area and village and highway, to prevent that town and country dust, waste water, waste gas and too much human activity from bringing pollution to Poria cocos.
Desinsection, put soil in order needs to carry out soil disinfection with killing termite medicine later, prevents that termite moth stings pine tree.
Wood is got the raw materials ready, the dry section wood method of getting the raw materials ready selects the above good-for-nothing pine tree of diameter 12cm to cut down, or after drawing materials, stays end to end, strikes off small stems, 10 centimetres of above pine trees of diameter are rived into 6-8 sheet, 6 centimetres of diameters are following rives into 2-4 sheet, rives and removes part bark rather than whole barks, through 10～20 days, dries, be sawn into the segment of long 70～80cm, and press " well " font stacking in dry area without shade, and section grillage upper cover forage, must cover plastic film rainy season.
The pine tree of pine tree is the black pine in Pinaceae pine genus plant, Chinese pine, Korean pine, Huashan pine, pinus yunnanensis, pinus khasys, masson pine etc., the most area of China is covered with masson pine and Chinese pine, the present invention can suit measures to local conditions, and selecting local good-for-nothing pine tree is raw material.
Inoculation, in whole good planting site, along slope aspect trench digging, the earth in ditch has been mixed control termites medicine, lays out pine tree section, pine tree section cuts or cuts out osculum, by expanding the spile that covers with Poria cocos mycelia of cultivating, be implanted in pine tree section, implant the intersegmental 5-10 centimetre that is divided into of pine tree of mycelia, juggle top covers clean pine tree fragment or pine trunk thin slice, then earthing 10～15cm, put up mat shelter above, keep the temperature 20-30 ℃ of soil, water content 20-40%.
Field management, latter 7～10 days of inoculation, just can grow white Poria cocos mycelia, at this moment should check the bacterium feelings in each location, to fill a vacancy in time.After Poria cocos inoculation, 1-2 is individual month, loses basal plane and grows mycelia, does not also smell and has Poria cocos smell, reseed.Reseed be by do not go up bacterium section wood all dig out, dry moisture, wound was cut again, separately connect bacterial classification.
Inoculate after 7～10 days, if find termite, should be immediately with killing termite medicine, be sprayed at termite with it on Huo Yi road, prevent and treat.
Poria cocos forms after sclerotium (knot Siberian cocklebur), and as found, Poria cocos bassets and should earth up in time and weeding, and will note draining rainy season.
The processing of gathering, Poria cocos is 8～10 months maturations after inoculation generally.The Poria cocos of gathering is deposited in to indoor lee, with straw, covers tightly and place 5～6 days, then spread out and dry in the air 1～2 day.After moisture evaporation, again pile up, then cover tightly and put after 5～6 days with straw, peeling, puts into food steamer water proof and cook to the saturating heart, or water boils to the saturating heart, while boiling, will change water 3～4 times, then dice, is placed in bamboo mat or bamboo sieve, on cover 1 blank sheet of paper, put under sunlight and shine to absolutely dry, in case of overcast and rainy available charcoal fire is dried or heated-air drying, but the baking of unavailable naked light, avoid sootiness to make the flavescence of Poria cocos sheet, affect product quality.
Substantive distinguishing features of the present invention and significant progressive:
1, from the Poria cocos of the firm results of stable high yield, choose seeds, adopt the fine quality that adapts to local temperature, weather, guaranteed strain quality;
2, to soil acidity or alkalinity adjustment, with lime and ferrous sulfate or humic acid organic fertilizer adjustment soil pH value.
3, adopt the own medium through studying for many years, corn flour 30-50 weight portion, wheat bran 15～20 weight portions, manioc waste 20-40 weight portion, waste molasses of sugar plant 10-20 weight portion, potassium dihydrogen phosphate 5-18 weight portion, magnesium sulfate 1-3 weight portion, ferrous sulfate 1-3 weight portion, pine sawdust 150-200 weight portion, guarantees to expand the cultivation of bacterial classification.Particularly manioc waste, is the waste material of starch factory, contains a small amount of starch, can be salvaged.
4, adopt rational administrative skill, guarantee good quality and high output.
Embodiment
Embodiment 1
The inventor carries out artificial planting Poria cocos in Mashan township, Heng County, Guangxi Zhuang Autonomous Region, and concrete steps are as follows:
The high-quality Poria cocos kind of the adaptation local temperature from the firm results of local stable high yield in 2012, weather, choose single heavy more than 2.5 kilograms large Siberian cocklebur, thalline is subsphaeroidal, and crust is thinner, light yellow or brownish red.Grow vigorous, appearance has obvious crackle.Inner Siberian cocklebur meat is white, and juice is more-and Poria cocos smell is strong, crust damages without insect bite, and without the difference of rotting, the kind Siberian cocklebur of collection is buried with wet lapped, with anti-drying.
Then by the Poria cocos meat in the middle of getting after the kind Siberian cocklebur surface clean of choosing and sterilization, on the medium of access test tube slant or triangular flask, move in 22～25 ℃ of insulating boxs and cultivate 5-7 days, obtain bacterial classification; Miscellaneous bacteria is wherein scalded miscellaneous bacteria dead or digs out processing with scorching hot inoculation shovel.Specifically the above-mentioned kind Siberian cocklebur of choosing is rinsed extremely without silt with clear water, with gauze, dry, then at the indoor 50-70% alcohol of inoculation, rinse whole bacterium ball, carry out surface sterilization, then use aseptic water washing 2-4 time, after kind of Siberian cocklebur surface dry, with aseptic cutter, be cut into two halves, the whiter Siberian cocklebur meat of picking soya bean size, on the medium of access test tube slant or triangular flask, labelled after inoculation, indicate kind of Siberian cocklebur source and inoculation time etc.Move in 22～25 ℃ of insulating boxs and cultivate, 5-7 days, obtains bacterial classification.
The potato agar medium that uses, fills a prescription as 200 grams, potato or Ipomoea batatas, 30 grams of glucose, 20 grams, agar, potassium dihydrogen phosphate l gram, 0.3 gram, magnesium sulfate, 0.5 gram, ferrous sulfate, 1000 milliliters, water; Medium needs sterilizing after carrying out.
Obtaining after bacterial classification, can expand cultivation, is pine to be cut into the fragment of 0.5～3 centimetre of size, 110～125 ℃ of steam sterilizings, 20～30 minutes, expand the access of Poria cocos mycelia in medium and fragment and cultivate, in the thermostatic chamber of 20～28 ℃, cultivate 5-10 days, mycelia is covered with media surface; After 5-7 days, the mycelia that bacterial classification is cultivated is covered with media surface and can expand cultivation, pine is cut into the fragment of 0.5～3 centimetre of size, 110～125 ℃ of steam sterilizings, 20～30 minutes, mix evenly with medium, be placed in flat board upper, the access of Poria cocos mycelia is expanded to medium culture.
Medium of the present invention is the medium through test of many times, for corn flour 30-50 weight portion, wheat bran 15～20 weight portions, manioc waste 20-40 weight portion, waste molasses of sugar plant 10-20 weight portion, potassium dihydrogen phosphate 5-18 weight portion, magnesium sulfate 1-3 weight portion, ferrous sulfate 1-3 weight portion, pine sawdust 150-200 weight portion, add water mixing and make its water content 60-90%, 110～125 ℃ of steam sterilizings, 20～30 minutes.
When expanding cultivation bacterial classification, we carry out whole ground to soil, select well-drained hillside, and soil is take yellow ground soil as main; Or yellow clay is added to 1/3～2/3 fine sand mixing.
Acid-base value is adjusted, and Poria cocos is liked faintly acid soil, and pH value is 4.5 to 6.5; So will be according to the soil property situation of planting site,, alkaline Organic Manure on Soil acid-base value acid with lime, aluminum sulfate, ferrous sulfate or band adjusted, if below 4.5, can suitably use a small amount of lime, regulate pH to reduce some heavy metal element harm.And during the too high soil of alkalescence, can add a small amount of aluminum sulfate, ferrous sulfate, humic acid fertilizer etc., soil is increased acid.Content of beary metal must strictly be controlled, and also requires away from city and industrial area and village and highway, to prevent that town and country dust, waste water, waste gas and too much human activity from bringing pollution to Poria cocos.
Desinsection, puts soil in order and carries out soil disinfection with killing termite medicine, prevents that termite moth stings pine tree.
Wood is got the raw materials ready, the dry section wood method of getting the raw materials ready selects the above good-for-nothing Chinese pine pine tree of diameter 12cm to cut down, or after drawing materials, stay end to end, striking off small stems, 10 centimetres of above pine trees of diameter are rived into 6-8 sheet, 6 centimetres of diameters are following rives into 2-4 sheet, rive and remove part bark rather than whole bark, through 10～20 days, dry, be sawn into the segment of long 70～80cm, and press " well " font stacking in dry area without shade, section grillage upper cover forage and plastic film are rainproof.
Inoculation, in whole good planting site, along slope aspect trench digging, the earth in ditch has been mixed control termites medicine, lays out pine tree section, pine tree section cuts or cuts out osculum, by expanding the spile that covers with Poria cocos mycelia of cultivating, be implanted in pine tree section, implant the intersegmental 5-10 centimetre that is divided into of pine tree of mycelia, juggle top covers clean pine tree fragment or pine trunk thin slice, then earthing 10～15cm, put up mat shelter above, keep the temperature 20-30 ℃ of soil, water content 20-40%.
Field management, latter 7～10 days of inoculation, just can grow white Poria cocos mycelia, at this moment should check the bacterium feelings in each location, to fill a vacancy in time.After Poria cocos inoculation, 1-2 is individual month, loses basal plane and grows mycelia, does not also smell and has Poria cocos smell, reseed.Reseed be by do not go up bacterium section wood all dig out, dry moisture, wound was cut again, separately connect bacterial classification.
Inoculate after 7～10 days, if find termite, should be immediately with killing termite medicine, be sprayed at termite with it on Huo Yi road, prevent and treat.
Poria cocos forms after sclerotium (knot Siberian cocklebur), and as found, Poria cocos bassets and should earth up in time and weeding, and will note draining rainy season.
The processing of gathering, Poria cocos is 8～10 months maturations after inoculation generally.The Poria cocos of gathering is deposited in to indoor lee, with straw, covers tightly and place 5～6 days, then spread out and dry in the air 1～2 day.After moisture evaporation, again pile up, then cover tightly and put after 5～6 days with straw, peeling, puts into food steamer water proof and cook to the saturating heart, or water boils to the saturating heart, while boiling, will change water 3～4 times, then dice, is placed in bamboo mat or bamboo sieve, on cover 1 blank sheet of paper, put under sunlight and shine to absolutely dry, in case of overcast and rainy available charcoal fire is dried or heated-air drying, but the baking of unavailable naked light, avoid sootiness to make the flavescence of Poria cocos sheet, affect product quality.。
The Poria cocos product that above-described embodiment obtains meets national Specification, and without fertilizer and pesticide, heavy metal free, pollutes without harmful chemical, on market, has sale at home at present, obtains the welcome on market.
Claims (3)
1. an implantation methods for Poria cocos, is characterized in that, comprise bacterial classification seed selection, bacterial classification cultivate, expand cultivate, soil is got the raw materials ready with acid-base value adjustment, desinsection, wood wholely, inoculation, field management, damage by disease and insect are prevented and kill off, the processing of gathering, obtains the process of high-quality Poria cocos,
Described bacterial classification seed selection is to choose single heavy more than 2.5 kilograms large Siberian cocklebur from the Poria cocos of the firm results of stable high yield, the special fine quality that adapts to local temperature, weather that adopts;
It is the Poria cocos meat by the middle of getting after the above-mentioned kind Siberian cocklebur surface clean of choosing and sterilization that described bacterial classification is cultivated, and on the medium of access test tube slant or triangular flask, moves in 22～25 ℃ of insulating boxs and cultivates 5-7 days, obtains bacterial classification;
Miscellaneous bacteria is wherein scalded miscellaneous bacteria dead or digs out processing with scorching hot inoculation shovel;
It is pine to be cut into the fragment of 0.5～3 centimetre of size that described expansion is cultivated, 110～125 ℃ of steam sterilizings, 20～30 minutes, be placed on flat board, the access of Poria cocos mycelia is expanded in medium and fragment and cultivated, in the thermostatic chamber of 20～28 ℃, cultivate 5-10 days, mycelia is covered with media surface;
Wholelyly and be to select well-drained hillside, soil is take yellow ground soil as main for described soil; Or yellow clay is added to 1/3～2/3 fine sand mixing;
Described acid-base value adjustment, be the soil property situation according to planting site,, alkaline Organic Manure on Soil acid-base value acid with lime, aluminum sulfate, ferrous sulfate or band adjusted, and content of beary metal must strictly be controlled, also require away from city and industrial area, prevent from polluting;
Described desinsection is with killing termite medicine, to carry out soil disinfection to putting soil in order later, prevents that termite moth stings pine tree;
It is to select the above good-for-nothing pine tree of diameter 12cm to cut down that described wood is got the raw materials ready, or after drawing materials, stay end to end, strike off small stems, rive into 2-8 sheet, after riving, remove part bark but want reserve part bark, dry, be sawn into segment, stacking is in dry area without shade, section grillage upper cover forage, and must cover plastic film rainy season;
Described inoculation is along slope aspect, to ditch in whole good planting site, earth in ditch has been mixed control termites medicine, lay out pine tree section, pine tree section cuts or cuts out osculum, by expanding the spile that covers with Poria cocos mycelia of cultivating, be implanted in pine tree section, implant back upper place and cover clean pine tree fragment or pine trunk thin slice, then earthing, the temperature 20-30 ℃ that keeps soil, water content 20-40%;
?described field management, is latter 7～10 days of inoculation, checks the bacterium feelings in each location, to fill a vacancy in time;
After Poria cocos inoculation, 1-2 is individual month, loses basal plane and grows mycelia, does not also smell and has Poria cocos smell, reseed;
Poria cocos forms after sclerotium, and as found, Poria cocos bassets and should earth up in time and weeding, and will note draining rainy season;
The described processing of gathering, is that the Poria cocos of gathering is deposited in to indoor lee, with straw, covers tightly and places 5～6 days, then spreads out and dries in the air 1～2 day, after moisture evaporation, again pile up, then cover tightly and put after 5～6 days with straw, peeling, put into food steamer water proof and cook, then stripping and slicing, dries, or heated-air drying.
2. the implantation methods of Poria cocos according to claim 1, is characterized in that, the medium that described bacterial classification is cultivated is: 200 grams, potato or Ipomoea batatas, 30 grams of glucose, 20 grams, agar, potassium dihydrogen phosphate l gram, 0.3 gram, magnesium sulfate, 0.5 gram, ferrous sulfate, 1000 milliliters, water; Medium needs sterilizing after carrying out.
3. the implantation methods of Poria cocos according to claim 1, is characterized in that, described expansion medium is: corn flour 30-50 weight portion, wheat bran 15～20 weight portions, manioc waste 20-40 weight portion, waste molasses of sugar plant 10-20 weight portion, potassium dihydrogen phosphate 5-18 weight portion, magnesium sulfate 1-3 weight portion, ferrous sulfate 1-3 weight portion, pine sawdust 150-200 weight portion, adds water mixing and makes its water content 60-90%, 110～125 ℃ of steam sterilizings, 20～30 minutes.
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Effective date of registration: 20211227 Address after: 100000 north of Dongshaoqu village, Dongshaoqu Town, Miyun District, Beijing Patentee after: Beijing Zhi Shen Tang Pharmaceutical Co.,Ltd. Address before: 530303 No. 156, weishanchun village, Xinlong village, Mashan Township, Heng County, Nanning City, Guangxi Zhuang Autonomous Region Patentee before: Chen Yonglin |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 |