CN104756766A - Method for culturing poria cocos through greenhouse - Google Patents
Method for culturing poria cocos through greenhouse Download PDFInfo
- Publication number
- CN104756766A CN104756766A CN201510185284.0A CN201510185284A CN104756766A CN 104756766 A CN104756766 A CN 104756766A CN 201510185284 A CN201510185284 A CN 201510185284A CN 104756766 A CN104756766 A CN 104756766A
- Authority
- CN
- China
- Prior art keywords
- poria cocos
- green house
- bag
- pine
- bacterium bag
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a method for culturing poria cocos through a greenhouse. The method includes the following steps of 1, manufacturing a cut log; 2, manufacturing auxiliary materials; 3, manufacturing cultispecies; 4, conducting inoculation; 5, selecting and leveling a land; 6, erecting the greenhouse; 7, arranging a termite trapping point and 8, conducting soil-cover culture. The method is low in production cost, the poria cocos can be cultured in all seasons, the growth cycle is short, the biotransformation rate is high, and remarkable ecological benefits and economical benefits are achieved.
Description
Technical field
The present invention relates to the technical field of cultivation of Poria cocos, be specifically related to a kind of method utilizing green house to cultivate Poria cocos.
Background technology
Poria cocos genus polyporus Cordycepps plant, is born in red, masson pine root more.Crust is thin and coarse, in sepia to pitchy, has obvious shrinkage texture.Dry sclerotia is used as medicine.The main component of Poria cocos is pachyman, pachymic acid, protein, histidine, lysergol, sylvite etc., has effect of clearing damp and promoting diuresis, spleen-benefiting mind-tranquilizing.The research data of numerous medical research unit proves, Poria cocos has the multiple element useful to human body, i.e. " anti-ageing element " and " anticancer element ".Therefore, cultivation and the series exploitation energetically of Poria cocos have fabulous economic benefit, social benefit and wide market prospects.
China's artificial cultivation pine Poria cocos has the history of more than one thousand years, last century early sixties just someone carry out large area artificial cultivation pine Poria cocos, but in existing culture technique, the biological transformation ratio of cultivated species is only 15%-20%, growth cycle is long, usually just can gather at 200-240 days, and raw-material consumption is comparatively large, often produces one ton of fresh Poria cocos, need 5 tons of pine raw material, production cost is high.
Conventional soil covering culture method is adopted in prior art, under open-air condition, Bag Material temperature and soil moisture all can not control, need winter many blindings antifreeze, summer, then temperature was too high, needed spray cooling, stationary temperature and humidity cannot be kept, rainy day too much also can cause rotten Siberian cocklebur, output reduce, and people and animals step on also can have a strong impact on Poria cocos grow.
Application number be 201010569073.4 patent discloses a kind of indoor bag cultivation method of poria, bacterium bag is put in indoor sand ground and cultivates, but the limited area of indoor sand ground, be not suitable for implant mass Poria cocos, and the venue cost of sand ground is higher.
Summary of the invention
For above-mentioned weak point of the prior art, the present invention aims to provide a kind of method utilizing green house to cultivate Poria cocos, and production cost of the present invention is low, the four seasons all can cultivate Poria cocos, growth cycle is short, biological transformation ratio is high.
To achieve these goals, technical scheme of the present invention: a kind of method utilizing green house to cultivate Poria cocos, the method comprises the following steps:
1) make Duan Mu: choose the pine tree wood that fresh nothing goes mouldy, be sawn into the Duan Mu of 33-35 centimeter length, the tertia drying pruned, is bundled into the dry and soft juggle of 5kg/ bundle;
2) make auxiliary material: by percentage by weight be the absolutely dry pine sawdust of 77%, the rice bran of 20% or wheat bran, 1% white sugar, 1% gypsum, 0.5% superphosphate, 0.5% magnesium sulfate mixing, the water adding the 110-120% of said mixture gross weight again stirs, for subsequent use;
3) make cultivated species: first by step 1) dry and soft juggle put into clear water and soak 10-12 hour, pick up and drain excessive moisture, after after soaking, the water content of pine section reaches 60%, again by above-mentioned steps 2) in auxiliary material fully mix thoroughly, keep the interior water content of material 60%, the thick auxiliary material of 5cm is loaded in the PEF plastic sack of 30 cm x 60 cm x 6.5 decimillimeters, again the pine section of soaking is loaded in bag, bag internal upper part loads the thick auxiliary material of 5cm equally, tie sack, carry out high-temperature sterilization;
4) inoculate: bacterium bag good for sterilizing is tripped out in autoclave, put into clean operating room, in bag subject to sterilization, temperature drops to after below 28 DEG C, again bacterium bag is moved into the inoculation that inoculating hood aseptically carries out Poria cocos secondary kind, the increasing of putting into 26-28 DEG C after connecting kind is in time supported indoor and is carried out constant temperature culture, after 20-30 days, mycelia covers with full bag, obtained bacterium bag;
5) select and arrange place: select on the sunny side, loosing soil, the gradient lower than 60 °, the good plot of drainage as milpa, deep-cut place 30-50 centimetre, whole fine earth earth;
6) green house is built: on the place put in order, build green house, and dig drain ditch;
7) termite-killing trapping point is arranged: deep-cut in the surrounding on every mu of ground that 4-6 floor space is 1-1.5 ㎡, the degree of depth is the heatable adobe sleeping platform of 60cm, fuel and the Poria cocos bacterial classification of termite eating is stacked in hole, spray termite spirit powder or aqua termite medicine, add and build fresh Folium et Ramulus Pini, then cover with soil;
8) soil covering culture: by step 4) in the film of obtained bacterium bag one end scratch a Tiaokou, to opening part insert one fresh absolutely dry and branch that is long 30cm draws wood, again bacterium bag is thrown into along slope in the heatable adobe sleeping platform of above-mentioned green house, often row heatable adobe sleeping platform is wide one meter, often discharge two bag bacterium bags, bacterium bag is inserted with and draws the sidewall of wooden one end near heatable adobe sleeping platform, blinding 10-15 cm thick, keep temperature in material to be 28-30 DEG C, in soil, water content is 55-70%.
Preferably, described pine tree wood is pine roots, pine tree pocket, pine tree tail or pine branch leftover pieces.
Preferably, described step 3) in high-temperature sterilization be high pressure-temperature sterilizing, by bacterium bag sterilizing 4-5 hour under the pressure of 0.15MPa.
Preferably, described step 3) in high-temperature sterilization be constant-pressure and high-temperature sterilizing, by bacterium bag sterilizing 14-16 hour at 100 DEG C, rest after truce 6-8 hour.
Preferably, described step 5) place be orcharding, broad-leaved forest land, nonirrigated farmland or mountain area, rural area ground.
Preferably, described step 7) in greenhouse booth be stamped sunshade net in summer.
Preferably, described step 7) in greenhouse booth be stamped plastic film in the winter time.
Preferably, described step 7) in soil moisture content be 55-65%.
Preferably, described step 7) in soil moisture content be 60%.
Beneficial effect of the present invention: 1. strain bio conversion ratio of the present invention reaches 65%-120%, improve 4-6 times than 15-20% of conventional cultivation method biological transformation ratio, production cost 100 jin raw materials more every than conventional cultivation need 31 yuan and have been reduced to again 29 yuan, reduce 6.45%.
2. the availability of resource reaches 100%, leftover bits after pine roots, the pine tree root and stem of certain plants, pine branch, pine tree tail and processing, pine sawdust can be used for cultivating Poria cocos, save pine raw material, cultivation method routinely, often produce 1 ton of fresh Poria cocos, need pine raw material 5 tons be used, after adopting cultivation method of the present invention, often produce 1 ton of fresh Poria cocos and only need use pine raw material 1.5 tons, 3.5 tons can be saved material than conventional cultivation method.
3. the growth cycle of Poria cocos is short, only has 150-170 days from being inoculated into gather, and than 200-240 days of conventional cultivation method, reduces 50-70 days.
4. the packing of Poria cocos product improves 5%, the yield of Poria cocos dry product brings up to 55-65% from 40-50%, particularly, the Poria cocos that existing implantation methods cultivates, be the obtained Poria cocos dry product of the Poria cocos processing of 10 jin to weight in wet base be 4-5 jin, adopting the Poria cocos that cultivates of the present invention, be the obtained Poria cocos dry product of the Poria cocos processing of 10 jin is 5.5-6.5 jin to weight in wet base, and practical experience proves that the packing of Poria cocos is improved significantly.
5. the Poria cocos of the present invention's cultivation in a year can gather in the crops 3 years continuously, and First Year realizes high yield, and reach per mu yield 8000 jin, Second Year realizes stable yields, reaches per mu yield 3000 jin, within the 3rd year, realizes suitable producing, reaches per mu yield 1500 jin.
6. the parasitosis rate of the Poria cocos of the present invention's cultivation is lower than 1%, and almost nil, the availability of Poria cocos is high, and the Poria cocos abandoned because of parasitosis does not almost have.
7. put up green house control temperature and humidity, reduce the evaporation of soil moisture, guarantee that Poria cocos grows under optimum temperature and humidity, not by the impact in season, all can cultivate Poria cocos throughout the year, according to practical experience, within 1 year, can plant two season Poria cocos.The present invention can realize intensive and factory culture Poria cocos, effectively controls the temperature and humidity of Poria cocos growth, prevents people and animals from stepping on, is convenient to management, does not need to use any agricultural chemicals, keeps the pure natural property of Poria cocos product.
8. solve artificial cultivation pine Poria cocos for a long time to cause the underproduction by white ant infestations and lose the technical problem received, easily promote.
The present invention has significant ecological benefits, the mycelium cultivation of the branch of masson pine waste wood, leaf, tree root, butt is utilized to grow Poria cocos, put up green house, packed nuisanceless factory culture, turns waste into wealth, and belongs to a new culture technique, Poria cocos pure natural property can be kept, the availability of masson pine waste wood can be improved again, improve the ecological environment, realize sustainable development.
Embodiment
The present invention is further described below in conjunction with specific embodiment.
Embodiment 1
Utilize green house to cultivate a method for Poria cocos, the method comprises the following steps:
1) make Duan Mu: choose the pine tree wood that fresh nothing goes mouldy, comprise pine roots, pine tree pocket, pine tree tail, pine branch leftover pieces, be sawn into the Duan Mu of 33-35 centimeter length, the tertia drying pruned, is bundled into the dry and soft juggle of 5kg/ bundle;
2) make auxiliary material: by percentage by weight be the absolutely dry pine sawdust of 77%, 20% rice bran, 1% white sugar, 1% gypsum, 0.5% superphosphate, 0.5% magnesium sulfate mixing, the water adding 110% of said mixture gross weight again stirs, for subsequent use;
3) make cultivated species: first by step 1) dry and soft juggle put into clear water and soak 10-12 hour, pick up and drain excessive moisture, after after soaking, the water content of pine section reaches 60%, again by above-mentioned steps 2) in auxiliary material fully mix thoroughly, keep the interior water content of material 60%, the thick auxiliary material of 5cm is loaded in the high density high pressure PEF plastic sack of 30 cm x 60 cm x 6.5 decimillimeters, again the pine section of soaking is loaded in bag, bag internal upper part loads the thick auxiliary material of 5cm equally, tie sack, carry out high-temperature sterilization, high-temperature sterilization can adopt high pressure-temperature sterilizing, be specially bacterium bag sterilizing 4-5 hour under the pressure of 0.15MPa, also constant-pressure and high-temperature sterilizing can be adopted, be specially bacterium bag sterilizing 14-16 hour at 100 DEG C, rest after truce 6-8 hour,
4) inoculate: bacterium bag good for sterilizing is tripped out in autoclave, put into clean operating room, in bag subject to sterilization, temperature drops to after below 28 DEG C, again bacterium bag is moved into inoculating hood, aseptically carry out the inoculation of Poria cocos secondary kind, the increasing of putting into 26 DEG C after connecting kind is in time supported indoor and is carried out constant temperature culture, and after 20-30 days, mycelia covers with full bag, obtained bacterium bag;
5) select and arrange place: select on the sunny side, loosing soil, the gradient lower than 60 °, the good plot of drainage is as milpa, place can be orcharding, broad-leaved forest land, nonirrigated farmland or ground, mountain area, rural area, deep-cut place 30-50 centimetre, whole fine earth earth, will dig deeply with accomplishing, soil wants carefully whole;
6) green house is built: on the place put in order, build green house, the dimensions of booth has multiple, and dig drain ditch, preferably, high 5.7 meters, wide 6 meters of booth, booth spacing 1-1.5 rice, the degree of depth and the width in drain ditch are 30-50 centimetre, are more preferably 40 cm x 40 centimetres;
7) termite-killing trapping point is arranged: deep-cut in the surrounding on every mu of ground that 4-6 floor space is 1-1.5 ㎡, the degree of depth is the heatable adobe sleeping platform of 60cm, in hole, stack fuel and the Poria cocos bacterial classification of termite eating, spray termite spirit powder or aqua termite medicine, add and build fresh Folium et Ramulus Pini, cover with soil again, trapping and killing termites;
8) soil covering culture: by step 4) in the film of obtained bacterium bag one end scratch a Tiaokou, to opening part insert one fresh absolutely dry and branch that is long 30cm draws wood, again bacterium bag is thrown into along slope in the heatable adobe sleeping platform of above-mentioned green house, often row heatable adobe sleeping platform is wide one meter, often discharge two bag bacterium bags, bacterium bag is inserted with and draws the sidewall of wooden one end near heatable adobe sleeping platform, blinding 10-15 cm thick, note high temperature, high humidity, prolonged drought or wet weather, on green house, sunshade net is put up during hot weather, on green house, plastic film is put up during microthermal climate, often regulate Bag Material temperature and soil moisture, temperature in material is kept to be 28-30 DEG C, in soil, water content is 55%.
After bacterium bag cultivating terminates, under normal temperature and humidity condition, within about 15 days, just start to tie Siberian cocklebur, now Poria cocos growth is very fast, growing native face and rotting because exposing to the sun and rain, affecting product yield and quality to prevent Poria cocos, often to note ridging, keep loosing soil and suitable water content.
After bacterium bag soil covering culture to knot Siberian cocklebur, 90-110 days Poria cocos growth and maturity under normal temperature, humidity growth conditions, its feature is that the knot soil layer at Siberian cocklebur place and the epidermis of Poria cocos do not have new crackle to occur, Siberian cocklebur is formed and easily gets loose with wooden, prove Poria cocos growth and maturity, must gather in time.Early have late because knot Siberian cocklebur has, accomplish ripe a collection of, gather a collection of.
Embodiment 2
Utilize green house to cultivate a method for Poria cocos, the method comprises the following steps:
1) make Duan Mu: choose the pine tree wood that fresh nothing goes mouldy, comprise pine roots, pine tree pocket, pine tree tail, pine branch leftover pieces, be sawn into the Duan Mu of 33-35 centimeter length, the tertia drying pruned, is bundled into the dry and soft juggle of 5kg/ bundle;
2) make auxiliary material: by percentage by weight be the absolutely dry pine sawdust of 77%, 20% wheat bran, 1% white sugar, 1% gypsum, 0.5% superphosphate, 0.5% magnesium sulfate mixing, the water adding 115% of said mixture gross weight again stirs, for subsequent use;
3) make cultivated species: first by step 1) dry and soft juggle put into clear water and soak 10-12 hour, pick up and drain excessive moisture, after after soaking, the water content of pine section reaches 60%, again by above-mentioned steps 2) in auxiliary material fully mix thoroughly, keep the interior water content of material 60%, the thick auxiliary material of 5cm is loaded in the high density high pressure PEF plastic sack of 30 cm x 60 cm x 6.5 decimillimeters, again the pine section of soaking is loaded in bag, bag internal upper part loads the thick auxiliary material of 5cm equally, tie sack, carry out high-temperature sterilization, high-temperature sterilization can adopt high pressure-temperature sterilizing, be specially bacterium bag sterilizing 4-5 hour under the pressure of 0.15MPa, also constant-pressure and high-temperature sterilizing can be adopted, be specially bacterium bag sterilizing 14-16 hour at 100 DEG C, rest after truce 6-8 hour,
4) inoculate: bacterium bag good for sterilizing is tripped out in autoclave, put into clean operating room, in bag subject to sterilization, temperature drops to after below 28 DEG C, again bacterium bag is moved into inoculating hood, aseptically carry out the inoculation of Poria cocos secondary kind, the increasing of putting into 27 DEG C after connecting kind is in time supported indoor and is carried out constant temperature culture, and after 20-30 days, mycelia covers with full bag, obtained bacterium bag;
5) select and arrange place: select on the sunny side, loosing soil, the gradient lower than 60 °, the good plot of drainage is as milpa, place can be orcharding, broad-leaved forest land, nonirrigated farmland or ground, mountain area, rural area, deep-cut place 30-50 centimetre, whole fine earth earth, will dig deeply with accomplishing, soil wants carefully whole;
6) green house is built: on the place put in order, build green house, the dimensions of booth has multiple, and dig drain ditch, preferably, high 5.7 meters, wide 6 meters of booth, booth spacing 1-1.5 rice, the degree of depth and the width in drain ditch are 30-50 centimetre, are more preferably 40 cm x 40 centimetres;
7) termite-killing trapping point is arranged: deep-cut in the surrounding on every mu of ground that 4-6 floor space is 1-1.5 ㎡, the degree of depth is the heatable adobe sleeping platform of 60cm, in hole, stack fuel and the Poria cocos bacterial classification of termite eating, spray termite spirit powder or aqua termite medicine, add and build fresh Folium et Ramulus Pini, cover with soil again, trapping and killing termites;
8) soil covering culture: by step 4) in the film of obtained bacterium bag one end scratch a Tiaokou, to opening part insert one fresh absolutely dry and branch that is long 30cm draws wood, again bacterium bag is thrown into along slope in the heatable adobe sleeping platform of above-mentioned green house, often row heatable adobe sleeping platform is wide one meter, often discharge two bag bacterium bags, bacterium bag is inserted with and draws the sidewall of wooden one end near heatable adobe sleeping platform, blinding 10-15 cm thick, note high temperature, high humidity, prolonged drought or wet weather, on green house, sunshade net is put up during hot weather, on green house, plastic film is put up during microthermal climate, often regulate Bag Material temperature and soil moisture, temperature in material is kept to be 28-30 DEG C, in soil, water content is 60%.
After bacterium bag cultivating terminates, under normal temperature and humidity condition, within about 15 days, just start to tie Siberian cocklebur, now Poria cocos growth is very fast, growing native face and rotting because exposing to the sun and rain, affecting product yield and quality to prevent Poria cocos, often to note ridging, keep loosing soil and suitable water content.
After bacterium bag soil covering culture to knot Siberian cocklebur, 90-110 days Poria cocos growth and maturity under normal temperature, humidity growth conditions, its feature is that the knot soil layer at Siberian cocklebur place and the epidermis of Poria cocos do not have new crackle to occur, Siberian cocklebur is formed and easily gets loose with wooden, prove Poria cocos growth and maturity, must gather in time.Early have late because knot Siberian cocklebur has, accomplish ripe a collection of, gather a collection of.
Embodiment 3
Utilize green house to cultivate a method for Poria cocos, the method comprises the following steps:
1) make Duan Mu: choose the pine tree wood that fresh nothing goes mouldy, comprise pine roots, pine tree pocket, pine tree tail, pine branch leftover pieces, be sawn into the Duan Mu of 33-35 centimeter length, the tertia drying pruned, is bundled into the dry and soft juggle of 5kg/ bundle;
2) make auxiliary material: by percentage by weight be the absolutely dry pine sawdust of 77%, 20% rice bran, 1% white sugar, 1% gypsum, 0.5% superphosphate, 0.5% magnesium sulfate mixing, the water adding 120% of said mixture gross weight again stirs, for subsequent use;
3) make cultivated species: first by step 1) dry and soft juggle put into clear water and soak 10-12 hour, pick up and drain excessive moisture, after after soaking, the water content of pine section reaches 60%, again by above-mentioned steps 2) in auxiliary material fully mix thoroughly, keep the interior water content of material 60%, the thick auxiliary material of 5cm is loaded in the high density high pressure PEF plastic sack of 30 cm x 60 cm x 6.5 decimillimeters, again the pine section of soaking is loaded in bag, bag internal upper part loads the thick auxiliary material of 5cm equally, tie sack, carry out high-temperature sterilization, high-temperature sterilization can adopt high pressure-temperature sterilizing, be specially bacterium bag sterilizing 4-5 hour under the pressure of 0.15MPa, also constant-pressure and high-temperature sterilizing can be adopted, be specially bacterium bag sterilizing 14-16 hour at 100 DEG C, rest after truce 6-8 hour,
4) inoculate: bacterium bag good for sterilizing is tripped out in autoclave, put into clean operating room, in bag subject to sterilization, temperature drops to after below 28 DEG C, again bacterium bag is moved into inoculating hood, aseptically carry out the inoculation of Poria cocos secondary kind, the increasing of putting into 28 DEG C after connecting kind is in time supported indoor and is carried out constant temperature culture, and after 20-30 days, mycelia covers with full bag, obtained bacterium bag;
5) select and arrange place: select on the sunny side, loosing soil, the gradient lower than 60 °, the good plot of drainage is as milpa, place can be orcharding, broad-leaved forest land, nonirrigated farmland or ground, mountain area, rural area, deep-cut place 30-50 centimetre, whole fine earth earth, will dig deeply with accomplishing, soil wants carefully whole;
6) green house is built: on the place put in order, build green house, the dimensions of booth has multiple, and dig drain ditch, preferably, high 5.7 meters, wide 6 meters of booth, booth spacing 1-1.5 rice, the degree of depth and the width in drain ditch are 30-50 centimetre, are more preferably 40 cm x 40 centimetres;
7) termite-killing trapping point is arranged: deep-cut in the surrounding on every mu of ground that 4-6 floor space is 1-1.5 ㎡, the degree of depth is the heatable adobe sleeping platform of 60cm, in hole, stack fuel and the Poria cocos bacterial classification of termite eating, spray termite spirit powder or aqua termite medicine, add and build fresh Folium et Ramulus Pini, cover with soil again, trapping and killing termites;
8) soil covering culture: by step 4) in the film of obtained bacterium bag one end scratch a Tiaokou, to opening part insert one fresh absolutely dry and branch that is long 30cm draws wood, again bacterium bag is thrown into along slope in the heatable adobe sleeping platform of above-mentioned green house, often row heatable adobe sleeping platform is wide one meter, often discharge two bag bacterium bags, bacterium bag is inserted with and draws the sidewall of wooden one end near heatable adobe sleeping platform, blinding 10-15 cm thick, note high temperature, high humidity, prolonged drought or wet weather, on green house, sunshade net is put up during hot weather, on green house, plastic film is put up during microthermal climate, often regulate Bag Material temperature and soil moisture, temperature in material is kept to be 28-30 DEG C, in soil, water content is 65%.
After bacterium bag cultivating terminates, under normal temperature and humidity condition, within about 15 days, just start to tie Siberian cocklebur, now Poria cocos growth is very fast, growing native face and rotting because exposing to the sun and rain, affecting product yield and quality to prevent Poria cocos, often to note ridging, keep loosing soil and suitable water content.
After bacterium bag soil covering culture to knot Siberian cocklebur, 90-110 days Poria cocos growth and maturity under normal temperature, humidity growth conditions, its feature is that the knot soil layer at Siberian cocklebur place and the epidermis of Poria cocos do not have new crackle to occur, Siberian cocklebur is formed and easily gets loose with wooden, prove Poria cocos growth and maturity, must gather in time.Early have late because knot Siberian cocklebur has, accomplish ripe a collection of, gather a collection of.
Embodiment 4
Utilize green house to cultivate a method for Poria cocos, the method comprises the following steps:
1) make Duan Mu: choose the pine tree wood that fresh nothing goes mouldy, comprise pine roots, pine tree pocket, pine tree tail, pine branch leftover pieces, be sawn into the Duan Mu of 33-35 centimeter length, the tertia drying pruned, is bundled into the dry and soft juggle of 5kg/ bundle;
2) make auxiliary material: by percentage by weight be the absolutely dry pine sawdust of 77%, 20% rice bran, 1% white sugar, 1% gypsum, 0.5% superphosphate, 0.5% magnesium sulfate mixing, the water adding 115% of said mixture gross weight again stirs, for subsequent use;
3) make cultivated species: first by step 1) dry and soft juggle put into clear water and soak 10-12 hour, pick up and drain excessive moisture, after after soaking, the water content of pine section reaches 60%, again by above-mentioned steps 2) in auxiliary material fully mix thoroughly, keep the interior water content of material 60%, the thick auxiliary material of 5cm is loaded in the high density high pressure PEF plastic sack of 30 cm x 60 cm x 6.5 decimillimeters, again the pine section of soaking is loaded in bag, bag internal upper part loads the thick auxiliary material of 5cm equally, tie sack, carry out high-temperature sterilization, high-temperature sterilization can adopt high pressure-temperature sterilizing, be specially bacterium bag sterilizing 4-5 hour under the pressure of 0.15MPa, also constant-pressure and high-temperature sterilizing can be adopted, be specially bacterium bag sterilizing 14-16 hour at 100 DEG C, rest after truce 6-8 hour,
4) inoculate: bacterium bag good for sterilizing is tripped out in autoclave, put into clean operating room, in bag subject to sterilization, temperature drops to after below 28 DEG C, again bacterium bag is moved into inoculating hood, aseptically carry out the inoculation of Poria cocos secondary kind, the increasing of putting into 28 DEG C after connecting kind is in time supported indoor and is carried out constant temperature culture, and after 20-30 days, mycelia covers with full bag, obtained bacterium bag;
5) select and arrange place: select on the sunny side, loosing soil, the gradient lower than 60 °, the good plot of drainage is as milpa, place can be orcharding, broad-leaved forest land, nonirrigated farmland or ground, mountain area, rural area, deep-cut place 30-50 centimetre, whole fine earth earth, will dig deeply with accomplishing, soil wants carefully whole;
6) green house is built: on the place put in order, build green house, the dimensions of booth has multiple, and dig drain ditch, preferably, high 5.7 meters, wide 6 meters of booth, booth spacing 1-1.5 rice, the degree of depth and the width in drain ditch are 30-50 centimetre, are more preferably 40 cm x 40 centimetres;
7) termite-killing trapping point is arranged: deep-cut in the surrounding on every mu of ground that 4-6 floor space is 1-1.5 ㎡, the degree of depth is the heatable adobe sleeping platform of 60cm, in hole, stack fuel and the Poria cocos bacterial classification of termite eating, spray termite spirit powder or aqua termite medicine, add and build fresh Folium et Ramulus Pini, cover with soil again, trapping and killing termites;
8) soil covering culture: by step 4) in the film of obtained bacterium bag one end scratch a Tiaokou, to opening part insert one fresh absolutely dry and branch that is long 30cm draws wood, again bacterium bag is thrown into along slope in the heatable adobe sleeping platform of above-mentioned green house, often row heatable adobe sleeping platform is wide one meter, often discharge two bag bacterium bags, bacterium bag is inserted with and draws the sidewall of wooden one end near heatable adobe sleeping platform, blinding 10-15 cm thick, note high temperature, high humidity, prolonged drought or wet weather, on green house, sunshade net is put up during hot weather, on green house, plastic film is put up during microthermal climate, often regulate Bag Material temperature and soil moisture, temperature in material is kept to be 28-30 DEG C, in soil, water content is 70%.
After bacterium bag cultivating terminates, under normal temperature and humidity condition, within about 15 days, just start to tie Siberian cocklebur, now Poria cocos growth is very fast, growing native face and rotting because exposing to the sun and rain, affecting product yield and quality to prevent Poria cocos, often to note ridging, keep loosing soil and suitable water content.
After bacterium bag soil covering culture to knot Siberian cocklebur, 90-110 days Poria cocos growth and maturity under normal temperature, humidity growth conditions, its feature is that the knot soil layer at Siberian cocklebur place and the epidermis of Poria cocos do not have new crackle to occur, Siberian cocklebur is formed and easily gets loose with wooden, prove Poria cocos growth and maturity, must gather in time.Early have late because knot Siberian cocklebur has, accomplish ripe a collection of, gather a collection of.
Above the technical scheme that the embodiment of the present invention provides is described in detail, apply specific case herein to set forth the principle of the embodiment of the present invention and embodiment, the explanation of above embodiment is only applicable to the principle helping to understand the embodiment of the present invention; Meanwhile, for one of ordinary skill in the art, according to the embodiment of the present invention, embodiment and range of application all will change, and in sum, this description should not be construed as limitation of the present invention.
Claims (9)
1. utilize green house to cultivate a method for Poria cocos, it is characterized in that, the method comprises the following steps:
1) make Duan Mu: choose the pine tree wood that fresh nothing goes mouldy, be sawn into the Duan Mu of 33-35 centimeter length, the tertia drying pruned, is bundled into the dry and soft juggle of 5kg/ bundle;
2) make auxiliary material: by percentage by weight be the absolutely dry pine sawdust of 77%, the rice bran of 20% or wheat bran, 1% white sugar, 1% gypsum, 0.5% superphosphate, 0.5% magnesium sulfate mixing, the water adding the 110-120% of said mixture gross weight again stirs, for subsequent use;
3) make cultivated species: first by step 1) dry and soft juggle put into clear water and soak 10-12 hour, pick up and drain excessive moisture, after after soaking, the water content of pine section reaches 60%, again by above-mentioned steps 2) in auxiliary material fully mix thoroughly, keep the interior water content of material 60%, the thick auxiliary material of 5cm is loaded in the PEF plastic sack of 30 cm x 60 cm x 6.5 decimillimeters, again the pine section of soaking is loaded in bag, bag internal upper part loads the thick auxiliary material of 5cm equally, tie sack, carry out high-temperature sterilization;
4) inoculate: bacterium bag good for sterilizing is tripped out in autoclave, put into clean operating room, in bag subject to sterilization, temperature drops to after below 28 DEG C, again bacterium bag is moved into the inoculation that inoculating hood aseptically carries out Poria cocos secondary kind, the increasing of putting into 26-28 DEG C after connecting kind is in time supported indoor and is carried out constant temperature culture, after 20-30 days, mycelia covers with full bag, obtained bacterium bag;
5) select and arrange place: select on the sunny side, loosing soil, the gradient lower than 60 °, the good plot of drainage as milpa, deep-cut place 30-50 centimetre, whole fine earth earth;
6) green house is built: on the place put in order, build green house, and dig drain ditch;
7) termite-killing trapping point is arranged: deep-cut in the surrounding on every mu of ground that 4-6 floor space is 1-1.5 ㎡, the degree of depth is the heatable adobe sleeping platform of 60cm, fuel and the Poria cocos bacterial classification of termite eating is stacked in hole, spray termite spirit powder or aqua termite medicine, add and build fresh Folium et Ramulus Pini, then cover with soil;
8) soil covering culture: by step 4) in the film of obtained bacterium bag one end scratch a Tiaokou, to opening part insert one fresh absolutely dry and branch that is long 30cm draws wood, again bacterium bag is thrown into along slope in the heatable adobe sleeping platform of above-mentioned green house, often row heatable adobe sleeping platform is wide one meter, often discharge two bag bacterium bags, bacterium bag is inserted with and draws the sidewall of wooden one end near heatable adobe sleeping platform, blinding 10-15 cm thick, keep temperature in material to be 28-30 DEG C, in soil, water content is 55-70%.
2. a kind of green house that utilizes cultivates the method for Poria cocos as claimed in claim 1, it is characterized in that, described step 1) in pine tree wood be pine roots, pine tree pocket, pine tree tail or pine branch leftover pieces.
3. a kind of green house that utilizes cultivates the method for Poria cocos as claimed in claim 1, it is characterized in that, described step 3) in high-temperature sterilization be high pressure-temperature sterilizing, by bacterium bag sterilizing 4-5 hour under the pressure of 0.15MPa.
4. a kind of green house that utilizes cultivates the method for Poria cocos as claimed in claim 1, it is characterized in that, described step 3) in high-temperature sterilization be constant-pressure and high-temperature sterilizing, by bacterium bag sterilizing 14-16 hour at 100 DEG C, rest after truce 6-8 hour.
5. a kind of green house that utilizes cultivates the method for Poria cocos as claimed in claim 1, it is characterized in that, described step 5) place be orcharding, broad-leaved forest land, nonirrigated farmland or ground, mountain area, rural area.
6. a kind of green house that utilizes cultivates the method for Poria cocos as claimed in claim 1, it is characterized in that, described step 7) in green house be stamped sunshade net in summer.
7. a kind of green house that utilizes cultivates the method for Poria cocos as claimed in claim 1, it is characterized in that, described step 7) in green house be stamped plastic film in the winter time.
8. a kind of green house that utilizes cultivates the method for Poria cocos as claimed in claim 1, it is characterized in that, described step 7) in soil moisture content be 55-65%.
9. a kind of green house that utilizes cultivates the method for Poria cocos as claimed in claim 8, it is characterized in that, described step 7) in soil moisture content be 60%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510185284.0A CN104756766A (en) | 2015-04-20 | 2015-04-20 | Method for culturing poria cocos through greenhouse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510185284.0A CN104756766A (en) | 2015-04-20 | 2015-04-20 | Method for culturing poria cocos through greenhouse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104756766A true CN104756766A (en) | 2015-07-08 |
Family
ID=53639158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510185284.0A Pending CN104756766A (en) | 2015-04-20 | 2015-04-20 | Method for culturing poria cocos through greenhouse |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104756766A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104973983A (en) * | 2015-07-29 | 2015-10-14 | 句容美华园林景观发展有限公司 | Poria cocos cultivation land soil remediation agent and preparation method thereof |
| CN105309203A (en) * | 2015-11-06 | 2016-02-10 | 道真自治县香坪中药材种植专业合作社 | Method for utilizing pine and small-diameter logs for planting poria cocos |
| CN105309201A (en) * | 2015-07-17 | 2016-02-10 | 靖州县绿环茯苓科技发展有限公司 | A Chinese tuckahoe raw material cultivation method |
| CN105917960A (en) * | 2016-04-21 | 2016-09-07 | 宁洱通达中药材种植专业合作社 | Planting method for poria cocos |
| CN107455148A (en) * | 2017-10-05 | 2017-12-12 | 杜方平 | A kind of fushen's cultural method |
| CN107624506A (en) * | 2017-10-05 | 2018-01-26 | 杜方平 | A kind of method using discarded pie pine section cultivation Poria cocos |
| CN108834750A (en) * | 2018-07-31 | 2018-11-20 | 庐山市环绿时代农业科技开发有限公司 | A kind of implantation methods of Poria cocos |
| CN111771606A (en) * | 2019-04-05 | 2020-10-16 | 杜方平 | Method for planting poria with hostwood in bark-free pine |
| CN112136598A (en) * | 2020-09-23 | 2020-12-29 | 重庆市中药研究院 | Efficient poria cocos planting method |
| CN114051887A (en) * | 2021-11-19 | 2022-02-18 | 云茯苓普洱有限公司 | Indoor planting method of poria cocos |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1732761A (en) * | 2005-07-29 | 2006-02-15 | 四川省农业科学院生物技术核技术研究所 | A kind of method of cultivating Poria cocos |
| CN101731100A (en) * | 2009-12-22 | 2010-06-16 | 王克勤 | Cultivating method of poria cocos bag material |
| CN103548578A (en) * | 2013-11-18 | 2014-02-05 | 陈永林 | Planting method for Chinese medicinal plant tuckahoe |
-
2015
- 2015-04-20 CN CN201510185284.0A patent/CN104756766A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1732761A (en) * | 2005-07-29 | 2006-02-15 | 四川省农业科学院生物技术核技术研究所 | A kind of method of cultivating Poria cocos |
| CN101731100A (en) * | 2009-12-22 | 2010-06-16 | 王克勤 | Cultivating method of poria cocos bag material |
| CN103548578A (en) * | 2013-11-18 | 2014-02-05 | 陈永林 | Planting method for Chinese medicinal plant tuckahoe |
Non-Patent Citations (6)
| Title |
|---|
| 何素明等: "松树"三剩物"生料栽培茯苓研究", 《北方园艺》 * |
| 李剑等: "茯苓棚室代料栽培技术研究初报", 《食用菌学报》 * |
| 王先有: "袋料栽培松茯苓 高产优质效益好", 《湖南科技报》 * |
| 胡敦松: "幼林套种茯苓 袋料栽培高产", 《湖南科技报》 * |
| 马巾媛等: "茯苓栽培技术研究", 《中国园艺文摘》 * |
| 魏新雨等: "松树"三剩料"袋料快速栽培茯苓技术", 《食药用菌》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309201A (en) * | 2015-07-17 | 2016-02-10 | 靖州县绿环茯苓科技发展有限公司 | A Chinese tuckahoe raw material cultivation method |
| CN104973983A (en) * | 2015-07-29 | 2015-10-14 | 句容美华园林景观发展有限公司 | Poria cocos cultivation land soil remediation agent and preparation method thereof |
| CN104973983B (en) * | 2015-07-29 | 2018-04-06 | 句容美华园林景观发展有限公司 | A kind of Poria cocos milpa soil-repairing agent and preparation method thereof |
| CN105309203A (en) * | 2015-11-06 | 2016-02-10 | 道真自治县香坪中药材种植专业合作社 | Method for utilizing pine and small-diameter logs for planting poria cocos |
| CN105917960A (en) * | 2016-04-21 | 2016-09-07 | 宁洱通达中药材种植专业合作社 | Planting method for poria cocos |
| CN107455148A (en) * | 2017-10-05 | 2017-12-12 | 杜方平 | A kind of fushen's cultural method |
| CN107624506A (en) * | 2017-10-05 | 2018-01-26 | 杜方平 | A kind of method using discarded pie pine section cultivation Poria cocos |
| CN108834750A (en) * | 2018-07-31 | 2018-11-20 | 庐山市环绿时代农业科技开发有限公司 | A kind of implantation methods of Poria cocos |
| CN111771606A (en) * | 2019-04-05 | 2020-10-16 | 杜方平 | Method for planting poria with hostwood in bark-free pine |
| CN112136598A (en) * | 2020-09-23 | 2020-12-29 | 重庆市中药研究院 | Efficient poria cocos planting method |
| CN114051887A (en) * | 2021-11-19 | 2022-02-18 | 云茯苓普洱有限公司 | Indoor planting method of poria cocos |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104756766A (en) | Method for culturing poria cocos through greenhouse | |
| CN105660143B (en) | A kind of Rhizoma Gastrodiae implantation methods using the artificial bacteria stick of Rhizoma Gastrodiae symbiosis halimasch | |
| CN107996288B (en) | Method for ecologically cultivating phellinus igniarius under forest by imitating wild conditions | |
| CN103704024B (en) | The method of the de-bag soil covering culture Dictyophora rubrovalvata of weed tree sawdust fermentation material grog bacterium rod | |
| CN104145718B (en) | A kind of bamboo grove simulated wild glossy ganoderma culture technique | |
| CN103563644B (en) | The cultivation method of a kind of large red glossy ganoderma | |
| CN103650921A (en) | Method for cultivating dictyophora rubrovolvata in bacteria stick bag-removing soil-covering mode through pine and China fir sawdust fermentation materials and sterilized materials | |
| CN105165513B (en) | A kind of production method of intercropping in garden mushroom | |
| CN102907260A (en) | Cultivation method of mulberry interplanted with ramulus mori ganoderma lucidum | |
| CN103493686B (en) | Method for cultivating tricholoma lobayense heim through mulberry twig stems and cassava stems | |
| CN103733874A (en) | Wildmimic cultivation method of ganoderma lucidum | |
| CN103416225A (en) | Method for preparing high-quality ganoderma lucidum compost | |
| CN103539540B (en) | Method for culturing tricholoma lobayense by using mulberry branches | |
| CN1282789A (en) | Method for cultivating glossy ganoderma on mulberry branch | |
| CN102351609A (en) | Culture material for black fungus cultivation | |
| CN103250550A (en) | Black fungus cultivation method and cultivation material thereof | |
| CN106171792A (en) | A kind of method of kiwi fruit tree garden interplanting Stropharia rugoso-annulata | |
| CN111788989A (en) | Method for interplanting stropharia rugoso-annulata under apple forest | |
| CN101953271B (en) | Imitation wild cultivation method of black fungus in jatropha curcas forest | |
| CN112690168A (en) | Novel method for cultivating stropharia rugoso-annulata under forest in poor mountain area | |
| CN107114120A (en) | The organic environmental-protection implantation methods of dictyophora phalloidea | |
| CN101983551A (en) | Cultivation method of king oyster mushroom | |
| CN105103943A (en) | Bionics wild cultivation method for lepista glaucocana | |
| CN103340091A (en) | Method for artificially cultivating xuefengensis | |
| CN103918482B (en) | A kind of cultivation method of Tricholoma Giganteum Massee |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150708 |