CN103340091A - Method for artificially cultivating xuefengensis - Google Patents
Method for artificially cultivating xuefengensis Download PDFInfo
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- CN103340091A CN103340091A CN2013102455030A CN201310245503A CN103340091A CN 103340091 A CN103340091 A CN 103340091A CN 2013102455030 A CN2013102455030 A CN 2013102455030A CN 201310245503 A CN201310245503 A CN 201310245503A CN 103340091 A CN103340091 A CN 103340091A
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Abstract
The invention discloses a method for artificially cultivating xuefengensis. According to the method, Ophiocordyceps xuefengensis is arranged on insect larva bodies of a furuncle bat moth family in a parasitic mode by T. C. Wen, J. C. Kang and R. C. Zhu. Plant folium isatidis is a host, a pleasant environment is built, and the xuefengensis cultivated artificially is obtained. The method for artificially cultivating the xuefengensis has the advantages that a cycle for cultivating the xuefengensis artificially only needs 1.5-3 months, and the cycle is shortened by more than 12 months in a natural state; the yield of the artificial xuefengensis is 7-10 times that of the artificial xuefengensis in a natural state; the quality of the artificial xuefengensis is improved, adenosine, mannitol, cordycepin, amino acid and the like in the artificial xuefengensis conform to the standards of cordyceps sinensis, and the content of the cordycepic acid is higher than that of the cordyceps sinensis.
Description
Technical field
The present invention relates to the method for a kind of artificial culture snowy peak Chinese caterpillar fungus.Especially utilize the Clavicipitaceae fungi autoeciousness to form the method for snowy peak Chinese caterpillar fungus at the Phassus nodus Chu et Wang of furuncle bat moth section insect larvae body.
Background technology
Cordyceps sinensis Ophiocordyceps.sinensis is the extraordinary traditional Chinese medicine of China, and Cordyceps sinensis is that sac fungi is invaded in the insect bodies such as Chinese caterpillar fungus bat moth and formed, and is the combination of a kind of worm and bacterium.Mainly be distributed in the alpine meadow soil of China Qinghai-Tibet Platean height above sea level 4000～5000m or the alpine scrub meadow soil, because the residing ecotope of Cordyceps sinensis is special, biology and the history of life complexity, so be in wild state for a long time, run its course, the natural propagation rate is very low, and resource is fewer and feweri, can not satisfy the demand far away, at home and abroad market becomes a kind of commodities in short supply.In recent years, under relevant department and scientific worker's effort, the ecology of Cordyceps sinensis and biological property and artificial breeding technique have been carried out a large amount of research, obtained a series of scientific achievement, the foundation of science is provided for the cultivation of Cordyceps sinensis.Chinese patent 01141386.7 discloses a kind of technology of cultivating Cordyceps sinensis with insect.Manually cultivate the fruit body of growth Cordyceps sinensis with the aweto fungus infected insect, its technical essential is: (1) at first cultivates the pure strong bacterial classification of Cordyceps sinensis: isolate cordyceps species with tissue isolation from wild bright Cordyceps sinensis, adopt the healthy cell breeding method to expand and connect bacterial classification and preserve standby; Cultivate female the kind and the liquid bacterium in inclined-plane that is used for infecting polypide; (2) select the host insect of susceptible microbiological contamination or for host insect for use, and spray bacterium liquid inductance dyes polypide; (3) cultivate in a bacterium stage and the stage simulation wild Chinese caterpillar fungus growth conditions that grows grass.Technology simply is convenient to popularize and promote; Artificial culture success Cordyceps sinensis can have been alleviated resource scarcity and the disparities between supply and demand of natural cordyceps.
Before 2010, the old scientific and technological work member Yuan Zihua of NAB National Association 0f Broadcasters of the township old docter of TCM of the Yao nationality of the county magistrate pool, hole, Hunan Province Yao nationality Dongkou County finds celestial grass (i.e. the snowy peak Chinese caterpillar fungus of naming now) in the smalt blade root of this locality.The snowy peak Chinese caterpillar fungus is a kind of special Chinese caterpillar fungus of low height above sea level, only at Hunan Province's Dongkou County discovery is arranged, and there is not report in other places.The inventor send Institute Of Chinese Materia Medica Of China Academy of Chinese Medical Sciences to do detection snowy peak Chinese caterpillar fungus sample, and with Cordyceps sinensis contrast, the result shows that the contained Main Ingredients and Appearance of snowy peak Chinese caterpillar fungus and Cordyceps sinensis are basic identical.The snowy peak Chinese caterpillar fungus is owing to be subjected to the influence of four big factors such as host, moth source, fungi, ecotope, resource scarcity.
In January, 2012, variety identification is carried out to the snowy peak Chinese caterpillar fungus in the Hunan Inst of Traditional Chinese Medicine, and the result shows that this product is Clavicipitaceae fungi snowy peak Chinese caterpillar fungus Ophiocordyceps xuefengensis T.C.Wen, J.C.Kang﹠amp; R.C.Zhu colonizes in stroma on the furuncle bat moth section insect larvae body and the dry composite body of larva corpse.Host plant is in Verenaceae plant smalt Clerodendrum near the ground marrow of cyrtophyllum Turcz. or the stem marrow.By 4 gene molecule Biology identification such as the RPBI gene of snowy peak Chinese caterpillar fungus, ATP gene, iTS5.8S gene are shown: be a novel species with the very near a kind of line style Cordyceps Ophiocordyceps sp. of Cordyceps sinensis Ophiocordyceps.sinensis affiliation.The Hunan Inst of Traditional Chinese Medicine is to Main Ingredients and Appearance and the content detection of snowy peak Chinese caterpillar fungus, and the result shows: 16 seed amino acid content, D-mannitol content, adenosine, cordycepin content all meet the standard code of Cordyceps sinensis.The content of cordycepic acid also is higher than Cordyceps sinensis.The animal acute toxicity test of snowy peak Chinese caterpillar fungus, long-acting toxicity test shows that all the snowy peak Chinese caterpillar fungus is a kind of Chinese caterpillar fungus of totally nontoxic.
Summary of the invention
The method that the purpose of this invention is to provide a kind of artificial culture snowy peak Chinese caterpillar fungus allows the snowy peak Chinese caterpillar fungus promote the well-being of mankind.
Technical scheme of the present invention is: with Clavicipitaceae fungi snowy peak Chinese caterpillar fungus Ophiocordyceps xuefengensis T.C.Wen, J.C.Kang﹠amp; R.C.Zhu is the host by manually colonizing on the furuncle bat moth section insect larvae body with the plant folium isatidis, forms the snowy peak Chinese caterpillar fungus through artificial culture.The output of snowy peak Chinese caterpillar fungus must have furuncle bat moth, folium isatidis is arranged, larva is arranged, the snowy peak cordyceps species is arranged, suitable ecotope is arranged, and five is indispensable.
The breeding method of snowy peak Chinese caterpillar fungus may further comprise the steps:
A, preparation composts or fertilisers of cultivating: by weight, 2～3 parts in weeds powder such as 1.5～2.5 parts of corn flour, wheat flour or 1.5～3 parts in wheat bran, 1～2 part of sucrose, glucose 3-5 part, 5～10 parts in yellow soil powder, 10～15 parts slightly of smalts, 0.5～1 part of Clavicipitaceae hypha,hyphae, 0.1～0.2 part of vitamin, lichens, dead leaf, raw material is mixed, and regulating the pH value is 5～7.Be cut into the snowy peak Chinese caterpillar fungus that is growing by knife fritter, pulverize into sesame shape grain piece then and mix with composts or fertilisers of cultivating and namely get the snowy peak cordyceps.
B, incubation step::
1) manyflower glorybower root that furuncle bat moth parasitism more than 3 ages is arranged on the mountain is dug out, keep smalt length 15～30cm, allow furuncle bat moth live wherein, do not have other insect;
2) the snowy peak aweto fungus is mixed with composts or fertilisers of cultivating, per 250～500g composts or fertilisers of cultivating mixes 3～6g snowy peak cordyceps;
3) vanning: 50～300 of every case dress smalts, every dress one deck sprinkles snowy peak Chinese caterpillar fungus powder 80～100g, and every case is adorned 4～5 layers, bore 2～4 holes on the case lid, bore dia 1～1.5cm builds case lid then, cover with calico or plastic film in the box top, chest and adjunct must meet sanitary standard;
4) check and observation: humidity 80～85% in the case; Temperature-5～30 degree, suitable Warm degree 10～25 degree; No natural daylight; Room ventilation is good; Deposit chest local pollution-free, no flue dust, noiselessness, no water damage, the no plague of rats, chest is nonvoluntary moves.
5) Chinese caterpillar fungus growth:
Drop cloth is taken out in vanning back 10～15 days, treats 20～40 days, the snowy peak Chinese caterpillar fungus that oneself forms is taken out, and mummification then, water content 5～7%, warehouse-in is preserved.
Good effect of the present invention is: the one, shorten snowy peak Chinese caterpillar fungus growth cycle, and the formation of snowy peak Chinese caterpillar fungus generally needs 12～18 months under the nature, and cultivation period of the present invention only needs 1.5～3 months; The 2nd, improve 7～10 times of snowy peak Chinese caterpillar fungus output, under the nature, every mu of smalt can produce snowy peak Chinese caterpillar fungus 0.1～0.3kg, and the snowy peak Chinese caterpillar fungus is produced in emulation of the present invention, and every per mu yield reaches 1.5～2.0kg; The 3rd, improve snowy peak Chinese caterpillar fungus quality, under the nature, because influences such as weather and the plague of rats, ant evils, the cordycepic acid in the snowy peak Chinese caterpillar fungus, cordycepin, protein are all undermined.Adenosine in the snowy peak Chinese caterpillar fungus of the present invention, mannitol, cordycepin all meet the standard code of Cordyceps sinensis; The 4th, take out the smalt of furuncle bat moth and can cultivate utilization again, survival rate 85～98%, useful and harmless to natural environment.The growth of snowy peak Chinese caterpillar fungus is different from Cordyceps sinensis, but snowy peak Chinese caterpillar fungus both snake throughout the year can be grown grass again.
Embodiment
The output of snowy peak Chinese caterpillar fungus must have furuncle bat moth, folium isatidis is arranged, larva is arranged, corresponding bacterial classification is arranged, suitable ecotope is arranged.Folium isatidis is a kind of shrub, and the different name smalt is used as medicine.Early on the books in books such as Compendium of Material Medica.Folium isatidis is unique host of snowy peak Chinese caterpillar fungus, therefore, at first will select to have on the mountain furuncle bat moth (more than 3 ages) to colonize in the folium isatidis of pith portion, and does not have pesticide pollution, there is no other insect.Next is ready to plastic box or wooden case, medium basin, thermometer, dry hygrometer, acid-base reagent, gloves, quicklime powder etc.
1, prepares composts or fertilisers of cultivating: by weight, 2～3 parts in weeds powder such as 1.5～2.5 parts of corn flour, wheat flour or 1.5～3 parts in wheat bran, 1～2 part of sucrose, glucose 3-5 part, 5～10 parts in yellow soil powder, 10～15 parts slightly of smalts, 0.5～1 part of Clavicipitaceae hypha,hyphae, 0.1～0.2 part of vitamin, lichens, dead leaf, raw material is mixed, and regulating the pH value is 5～7.
2, breeding method:
1) manyflower glorybower root is dug out, furuncle bat moth larvae is lived wherein, the long 15～30cm of smalt;
2) snowy peak Cordyceps Militaris and composts or fertilisers of cultivating are mixed and stirred, in 250～500g composts or fertilisers of cultivating, mix and stir 3～6g snowy peak Cordyceps Militaris powder;
3) vanning: with manyflower glorybower root pack into plastic box or wooden case, 50～300 in every case, every dress one deck sprinkles snowy peak Chinese caterpillar fungus powder 80～100g, every case is adorned 4～5 layers, bore 2～4 holes on the case lid, bore dia 1～1.5cm, cover lid then, cover with calico or plastic film in the box top, chest must meet sanitary standard.
4) check and observation: humidity 80～85% in the case; Temperature-5～30 degree, suitable Warm degree 10～20 degree; No natural daylight; Room ventilation is good; Deposit local pollution-free, no flue dust, noiselessness, no water damage, the no plague of rats, the no vehicle exhaust of chest, chest can not arbitrarily be moved.
5) snowy peak Chinese caterpillar fungus growth:
The smalt of band furuncle bat moth section larva was infected Clavicipitaceae fungi vanning back 10～15 days, drop cloth is taken out, treated 20～40 days, the snowy peak Chinese caterpillar fungus that oneself becomes is taken out, mummification then, water content 5～7%, warehouse-in is preserved.
In order to enlarge the cultivation of snowy peak Chinese caterpillar fungus, the way that we take is to educate the smalt sapling outside the mountain, snake in the mountain, the approach that grows grass in.Oneself plants more than 1000 mu of folium isatidis at present; and work out the cultivation management technology of folium isatidis; gathering of snowy peak Chinese caterpillar fungus bred series techniques such as management with storage technique, snowy peak Cordyceps Militaris and Chinese caterpillar fungus bat moth, is expected in the near future, and the snowy peak Chinese caterpillar fungus produces the established practice modelling and puts goods on the market.
Claims (2)
1. the method for artificial culture snowy peak Chinese caterpillar fungus is characterized in that: with Clavicipitaceae fungi snowy peak Chinese caterpillar fungus Ophiocordyceps xuefengensis T.C.Wen, J.C.Kang﹠amp; R.C.Zhu is the host by manually colonizing on the furuncle bat moth section insect larvae body with the folium isatidis, and the Artificial Control temperature and humidity forms the snowy peak Chinese caterpillar fungus through artificial culture.
2. the method for artificial culture snowy peak Chinese caterpillar fungus according to claim 1 may further comprise the steps:
A, preparation composts or fertilisers of cultivating: by weight, 2～3 parts in 1.5～2.5 parts of corn flour, wheat flour or 1.5～3 parts in wheat bran, 1～2 part of sucrose, glucose 2-5 part, 5～10 parts in yellow soil powder, 10～15 parts slightly at smalt end, 0.5～1 part of snowy peak aweto fungus mycelia, 0.1～0.2 part of vitamin, lichens and dead leaf weeds powder, raw material is mixed, and regulating the pH value is 5～7;
B, incubation step::
1) with on the mountain have furuncle bat moth more than 3 ages parasitic smalt tree root dig out and fracture, furuncle bat moth larvae is exposed, folium isatidis tree root length 15～30cm from xylem; There are not other insects;
2) the snowy peak aweto fungus is mixed with composts or fertilisers of cultivating, per 250～500g composts or fertilisers of cultivating mixes 3～6g snowy peak cordyceps;
3) vanning: 50～300 of every case dress smalt tree roots, every dress one deck sprinkles snowy peak Chinese caterpillar fungus powder 80～100g, and every case is adorned 4～5 layers, bore 2～4 holes on the case lid, bore dia 1～1.5cm, cover lid then, cover with calico or plastic film in the box top, chest must meet sanitary standard;
4) check and observation: humidity 80～85% in the case; Temperature 5～30 degree, suitable Warm degree 10～20 degree; No natural daylight; Room ventilation is good; Deposit chest local pollution-free, no flue dust, noiselessness, no water damage, the no plague of rats, chest is nonvoluntary moves.
5) snowy peak Chinese caterpillar fungus growth:
Drop cloth is taken out in vanning back 10～15 days, treat 20～40 days after, the snowy peak Chinese caterpillar fungus that oneself forms is taken out, mummification then, water content 5～7%, warehouse-in is preserved.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106047716A (en) * | 2016-06-14 | 2016-10-26 | 湖南师范大学 | Ophiocordyceps xuefengensis and its fruiting body and their artificial cultivation methods and application |
| CN106818206A (en) * | 2016-12-20 | 2017-06-13 | 湖南省中医药研究院 | A kind of artificial culturing method of snowy peak Chinese caterpillar fungus |
| CN107549120A (en) * | 2017-09-27 | 2018-01-09 | 湖南师范大学 | The rearing method and its larvae feeds formula of a kind of bat moth |
| CN109845580A (en) * | 2019-02-27 | 2019-06-07 | 湖南省蚕桑科学研究所 | A method of with 5 instar larvae of silkworm for host's artificial culture snowy peak cordyceps sinensis |
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| CN1414091A (en) * | 2001-10-26 | 2003-04-30 | 李健 | New technology of culturing cordyceps sinensis using insect |
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| CN101707966A (en) * | 2009-04-14 | 2010-05-19 | 徐正华 | Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047716A (en) * | 2016-06-14 | 2016-10-26 | 湖南师范大学 | Ophiocordyceps xuefengensis and its fruiting body and their artificial cultivation methods and application |
| CN106818206A (en) * | 2016-12-20 | 2017-06-13 | 湖南省中医药研究院 | A kind of artificial culturing method of snowy peak Chinese caterpillar fungus |
| CN107549120A (en) * | 2017-09-27 | 2018-01-09 | 湖南师范大学 | The rearing method and its larvae feeds formula of a kind of bat moth |
| CN109845580A (en) * | 2019-02-27 | 2019-06-07 | 湖南省蚕桑科学研究所 | A method of with 5 instar larvae of silkworm for host's artificial culture snowy peak cordyceps sinensis |
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