CN105660190A - Stereoscopic cultivation and breeding method for complementary symbiosis of ganoderma lucidum and wood frogs - Google Patents
Stereoscopic cultivation and breeding method for complementary symbiosis of ganoderma lucidum and wood frogs Download PDFInfo
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- CN105660190A CN105660190A CN201610110178.0A CN201610110178A CN105660190A CN 105660190 A CN105660190 A CN 105660190A CN 201610110178 A CN201610110178 A CN 201610110178A CN 105660190 A CN105660190 A CN 105660190A
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- 241000191896 Rana sylvatica Species 0.000 title claims abstract description 47
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 title claims abstract description 19
- 240000008397 Ganoderma lucidum Species 0.000 title abstract description 12
- 235000001637 Ganoderma lucidum Nutrition 0.000 title abstract description 11
- 238000009395 breeding Methods 0.000 title abstract description 3
- 230000000295 complement effect Effects 0.000 title abstract 4
- 238000012364 cultivation method Methods 0.000 title abstract 2
- 239000002689 soil Substances 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 241000222336 Ganoderma Species 0.000 claims description 45
- 238000000034 method Methods 0.000 claims description 11
- 239000003337 fertilizer Substances 0.000 claims description 10
- 241000243684 Lumbricus Species 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 239000004576 sand Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 4
- 238000013138 pruning Methods 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 241000238631 Hexapoda Species 0.000 claims description 3
- 230000037396 body weight Effects 0.000 claims description 3
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 235000021050 feed intake Nutrition 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 239000010871 livestock manure Substances 0.000 claims description 3
- 210000004681 ovum Anatomy 0.000 claims description 3
- 239000000575 pesticide Substances 0.000 claims description 3
- 239000010865 sewage Substances 0.000 claims description 3
- 235000003434 Sesamum indicum Nutrition 0.000 claims description 2
- 230000035800 maturation Effects 0.000 claims description 2
- 241000207961 Sesamum Species 0.000 claims 1
- 238000011081 inoculation Methods 0.000 claims 1
- 239000000843 powder Substances 0.000 abstract description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 abstract 2
- 235000012255 calcium oxide Nutrition 0.000 abstract 1
- 239000000292 calcium oxide Substances 0.000 abstract 1
- 238000010276 construction Methods 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 230000036561 sun exposure Effects 0.000 abstract 1
- 241001336827 Rana chensinensis Species 0.000 description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000002917 insecticide Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000132931 Batrachia Species 0.000 description 1
- 241000269799 Perca fluviatilis Species 0.000 description 1
- 241000269800 Percidae Species 0.000 description 1
- 244000000231 Sesamum indicum Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 210000003101 oviduct Anatomy 0.000 description 1
- -1 polyoxy Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000037384 skin absorption Effects 0.000 description 1
- 231100000274 skin absorption Toxicity 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mushroom Cultivation (AREA)
- Fertilizers (AREA)
Abstract
A stereoscopic cultivation and breeding method for complementary symbiosis of ganoderma lucidum and wood frogs is characterized by comprising the steps that a good, pure-natural and pollution-free area section is selected, it is ensured that the area section is free of residues, soil is not too alkaline and the area section is a loose sandy soil land section, deep land scarification is performed for 40 centimeters in the first year, leveling is performed, and a ganoderma lucidum and wood frog greenhouse is constructed after six months of sun exposure.Too large area is not allowed, the area of the greenhouse is 200 square meters, the length of the greenhouse is 25 meters, the width of the greenhouse is 8 meters, the height of the greenhouse is 1.9 meters, the area is suitable for a growing environment for the complementary symbiosis of the ganoderma lucidum and the wood frogs, the greenhouse is suitable for conidial powder recovery, the area of the greenhouse is suitable for the wood frogs due to the fact that wood frogs breathe through the skins, the ganoderma lucidum breathes freely, the wood frogs uptake oxygen, the complementary symbiosis is achieved, and the greenhouse is disinfected and sterilized with quick lime water after the greenhouse construction is completed; ganoderma lucidum mycelium in the step 2 is ganoderma lucidum.
Description
Technical field
The present invention relates to a kind of Ganoderma and wood frog complementation symbiosis solid cultivation cultural method.
Background technology
Wood frog is valued by the people day by day with its distinctive medical value and nutritive value, becomes the one that in batrachia, economic worth is the highest. In the wood frog that are produced from various places, again with Changbaishan area produce wood frog big with its physique, oil productivity is high, body constitution is healthy and strong, breeding potential is high and becomes the superfine product in wood frog. Oviductus Ranae is rich in 18 seed amino acids, mineral and multivitamin, nutrient substance contained by it is sufficient for the human body demand to various nutritional labelings, therefore has high nutrition and tonic effect name and is constantly recognized by people due to the distinctive Nutrition of wood frog itself and medical value. So, it is continuously developed production with the health product that wood frog is primary raw material, causes wood frog price to rise steadily. But due to wild wood frog limited amount. And constantly going to wreck property is destroyed, make wild wood frog resource be on the brink of the edge of extinction, seriously constrain the development of wood frog processing industry. Therefore, industrialization, scale forest frog culture will be solve this contradiction optimal path.
Ganoderma, also known as Ganoderma lucidum seu Japonicum, Ganoderma, Ganoderma lucidum seu Japonicum, Herba mesonae chinensis, Ganoderma, is the Herb of Polyporaceae plant Ganoderma lucidum (Leyss. Ex Fr.) Karst. or Ganoderma, owing to the existing soil content of organic matter is low, and the conventional Ganoderma implantation methods easy natural death of Ganoderma Second Year. So solving this difficult problem with Ganoderma and wood frog complementation symbiosis.
Summary of the invention
Based on above weak point, the present invention provides a kind of Ganoderma and wood frog complementation symbiosis solid cultivation cultural method. Solve the problem that Ganoderma is planted continuously, improve planting soil, make Ganoderma and wood frog complementation symbiosis, improve land utilization ratio, reduce cost, substantially increase the benefit of canopy room, accelerate wood frog and fatten, shorten the finished product phase.
The technology used in the present invention is as follows: a kind of Ganoderma and wood frog complementation symbiosis solid cultivation cultural method, as follows:
(1) selecting natural sand soil section, it is desirable to without pesticide, fertilizer residue, soil property does not have parlkaline, in advance soil subsoiling 40 centimeters, leveling, through the several months at sunshine, with building up symbiosis raising shed room after lime water sterilization;Canopy room area 200 square metres, long 25 meters, wide 8 meters is high 1.9 meters; Canopy inward flange surrounding arranges ditch, places pollution-free water, connect upper and lower circulating sewage, reach the purpose purified at any time in ditch;
(2) Ganoderma mycelium chooses matter is Ganoderma lucidum (Leyss. Ex Fr.) Karst., will Mycelium culture inoculate after be placed in bacteria canopy 45-60 days bacterias; Then soaked lobule Querci Mongolicae is cut into 12 centimeters long, is bundled into the culture that diameter is 20-30 centimetre, by cultured for Ganoderma mycelium transplanting on culture, put into described symbiosis raising shed room, every 1.4 square metres is one section, then covers the fine sand of 1 centimeter-1.5 centimeters, detains canopy shading; The isoperibol temperature cultivated is 28 DEG C, humidity 80-90%;
(3) treating that Ganoderma is broken ground 2-3 centimetre, carry out beta pruning, after beta pruning, put into wood frog, every square meter puts 70, body weight 15-25 gram, surface temperature 25-28 DEG C, air themperature 28-30 DEG C, humidity 80-90%;
(4) grow under half-light, feed living insects feedstuff, including fly larvae, Lumbricus, feed intake during the every day of the morning 8, on average every wood frog 5-10mg/ days, trophophase 135-150 days, it is managed going out sesame simultaneously; Ganoderma after maturation is manually plucked.
The present invention also has following technical characteristic: described step (2) is removed well the cattle manure of ammonia and is placed under cultivation of glossy ganoderma body by sterilization fermentation, makees Ganoderma base fertilizer and supports, and puts into Lumbricus ovum group in base fertilizer simultaneously, allows Nature creating Lumbricus in soil.
Because the Ganoderma place of artificial growth is more smooth, spacing in the rows is neat, blade drop docking wood frog habit. When to wood frog habitat moisturizing, wood frog habitat vegetation crop has been carried out effective moisturizing by nature again, kills two birds with one stone, and one throws multiple-effect, and how sharp a ground is. Because Ganoderma blade is full, water content is relatively sufficient, and leaf body is cool wet moderate, and oxygen flow molecule is higher. So, Rana temporaria chensinensis David perches growth under Ganoderma, wood frog can be made on land to perch basic wet environment higher all in ground vapour, shady in process, and the normal time can touch the Ganoderma blade that dampness is more, naturally dock wood frog and lean on habit and the required desirably site ecology condition of wood frog of skin absorption moisture content. Because the indoor various insecticides of the canopy of Ganoderma of planting are a lot of and like dropping on Ganoderma blade, the outstanding phase is obvious when rain, when high temperature, wind sky. This just makes insecticide and wood frog copolymerization one Room naturally, makes wood frog closely just can prey on bait (insecticide), decreases wood frog consumption of physical ability in bait process, accelerate wood frog and fatten, shorten the finished product phase. Trap foster Rana temporaria chensinensis David Ganoderma, the most directly, the most obvious effect be exactly the training allowing Rana temporaria chensinensis David accept Bao Zi powder, improve the medical value of Rana temporaria chensinensis David, more improve land utilization ratio, improve the content of soil with organic matter, reduce cost.
Detailed description of the invention
The present invention will be further described in citing below:
Embodiment 1
A kind of Ganoderma and wood frog complementation symbiosis solid cultivation cultural method are as follows:
(1) position is selected in north latitude 43-45 °, east longitude 124 degree-127 °, makes a clear distinction between the four seasons, and in rain heat same season, precipitation is relatively concentrated, and dry and wet is moderate. It it is the optimal living environment of Rana temporaria chensinensis David. Ganoderma canopy room air circulation nature polyoxy is moist, micro-cloudy light. Sooner or later the temperature difference about 15 degree, surface temperature, less than 28-30 DEG C, selects natural sand soil section, requiring without pesticide, fertilizer residue, soil property does not have parlkaline, in advance soil subsoiling 40 centimeters, leveling, through the several months at sunshine, with building up symbiosis raising shed room after lime water sterilization; Canopy room area 200 square metres, long 25 meters, wide 8 meters, high 1.9 meters, this area is suitable for Ganoderma wood frog complementation coupled growth environment, is suitable for Ganoderma spore powder and propagates;Canopy inward flange accomplishes the degree of depth 45 centimeters, width 80 centimeters, repairing of the wood frog pond (namely ditch) of length 25 meters, fills the water of pollution-free, the pure natural into 30 centimeters of degree of depth, connects upper and lower circulating sewage, reach the purpose that pond purifies at any time.
(2) Ganoderma mycelium chooses matter is Ganoderma lucidum (Leyss. Ex Fr.) Karst., will Mycelium culture inoculate after be placed in bacteria canopy 45-60 days bacterias; Then the lobule Querci Mongolicae dried is cut into 12 centimeters long, is bundled into the culture that diameter is 20-30 centimetre, Ganoderma is transplanted on culture, put into described symbiosis raising shed room, every 1.4 square metres is one section, then covers the fine sand of 1 centimeter-1.5 centimeters, detains canopy shading; The isoperibol temperature cultivated is 28 DEG C, humidity 80-90%; The cattle manure removing well ammonia by sterilization fermentation is placed under cultivation of glossy ganoderma body, make Ganoderma base fertilizer to support, base fertilizer is put into Lumbricus ovum group simultaneously, allows Nature creating Lumbricus in soil, namely play wood frog bait effect, play again loosening function, make Rana temporaria chensinensis David Excreta natural by soil absorption, improve soil, reduce pest and disease damage, improve Ganoderma yield and quality, reduce cost effect obvious.
(3) treating that Ganoderma is broken ground 2-3 centimetre, after beta pruning, put into wood frog, every square meter puts 70, body weight 15-25 gram, surface temperature 25-28 DEG C, air themperature 28-30 DEG C, humidity 80-90%;
(4) grow under half-light, feed living insects feedstuff, including fly larvae, Lumbricus, feed intake during the every day of the morning 8, on average every wood frog 5-10mg/ days, trophophase 135-150 days, accelerate wood frog and fatten, shorten the finished product phase. The Ganoderma mycelium produced is white, cap is bronzing, bacteria cover diameter can reach 15-18 centimetre size, the thickness of bacterial context can reach about 1.5 centimeters, the length of stem can reach 15-20 centimetre, the rugosity diameter of stem can reach 1.5-2 centimetre, and stem is hard, the temperature tolerance of mycelia can expand to 10-27 DEG C, but ambient temperature 25-30 DEG C that growth is suitable, the yield of Ganoderma and spore powder is also greatly improved and micronutrient levels increases considerably, and has good medicinal health value and ornamental value, improves planting soil.
Soil agrochemistry sample detection table
Detection unit: clay fertilizer station, Dorbod Mongol Autonomous County agricultural technology extension center.
Claims (2)
1. a Ganoderma and wood frog complementation symbiosis solid cultivation cultural method, it is characterised in that method is as follows:
(1) selecting natural sand soil section, it is desirable to without pesticide, fertilizer residue, soil property does not have parlkaline, in advance soil subsoiling 40 centimeters, leveling, through the several months at sunshine, with building up symbiosis raising shed room after lime water sterilization; Canopy room area 200 square metres, long 25 meters, wide 8 meters is high 1.9 meters; Canopy inward flange surrounding arranges ditch, places pollution-free water, connect upper and lower circulating sewage, reach the purpose purified at any time in ditch;
(2) will Ganoderma mycelium cultivate inoculation after be placed in bacteria canopy 45-60 days bacterias; Then the lobule Querci Mongolicae dried is cut into 12 centimeters long, is bundled into the culture that diameter is 20-30 centimetre, Ganoderma is transplanted on culture, put into described symbiosis raising shed room, every 1.4 square metres is one section, then covers the fine sand of 1 centimeter-1.5 centimeters, detains canopy shading; The isoperibol temperature cultivated is 28 DEG C, humidity 80-90%;
(3) treating that Ganoderma is broken ground 2-3 centimetre, after beta pruning, put into wood frog, every square meter puts 70, body weight 15-25 gram, surface temperature 25-28 DEG C, air themperature 28-30 DEG C, humidity 80-90%;
(4) grow under half-light, feed living insects feedstuff, including fly larvae, Lumbricus, feed intake during the every day of the morning 8, on average every wood frog 5-10mg/ days, trophophase 135-150 days, it is managed going out sesame simultaneously; Ganoderma after maturation is manually plucked.
2. a kind of Ganoderma according to claim 1 and wood frog complementation symbiosis solid cultivation cultural method, it is characterised in that: after the fermentation of step (2) administration of sterile, the cattle manure of deammoniation gas is as the base fertilizer of Ganoderma, puts into Lumbricus ovum group in base fertilizer simultaneously.
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| CN201610110178.0A CN105660190B (en) | 2016-02-29 | 2016-02-29 | Complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs |
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| CN201610110178.0A CN105660190B (en) | 2016-02-29 | 2016-02-29 | Complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs |
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Cited By (4)
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| CN107182734A (en) * | 2017-04-27 | 2017-09-22 | 江西九草铁皮石斛科技协同创新有限公司 | A kind of dendrobium candidum supports method with Ranaspinosa David set |
| CN107637385A (en) * | 2017-10-25 | 2018-01-30 | 四川三点水生物科技有限公司 | A kind of implantation methods of wild edible fungus |
| CN108834776A (en) * | 2018-07-16 | 2018-11-20 | 云南省农业科学院药用植物研究所 | A kind of implantation methods improving bigflower centranthera root tissue-cultured seedling transplanting survival rate |
| CN114027259A (en) * | 2021-12-03 | 2022-02-11 | 中国水产科学研究院黄海水产研究所 | Symbiotic cultivation method for Chinese wood frogs and burdock |
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| CN107637385A (en) * | 2017-10-25 | 2018-01-30 | 四川三点水生物科技有限公司 | A kind of implantation methods of wild edible fungus |
| CN108834776A (en) * | 2018-07-16 | 2018-11-20 | 云南省农业科学院药用植物研究所 | A kind of implantation methods improving bigflower centranthera root tissue-cultured seedling transplanting survival rate |
| CN114027259A (en) * | 2021-12-03 | 2022-02-11 | 中国水产科学研究院黄海水产研究所 | Symbiotic cultivation method for Chinese wood frogs and burdock |
| CN114027259B (en) * | 2021-12-03 | 2023-08-08 | 中国水产科学研究院黄海水产研究所 | Symbiotic cultivation method for Chinese wood frog and burdock |
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