CN105660190B - Complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs - Google Patents
Complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs Download PDFInfo
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- 241000191896 Rana sylvatica Species 0.000 title claims abstract description 62
- 241000222336 Ganoderma Species 0.000 title claims abstract description 20
- 230000000295 complement effect Effects 0.000 title claims abstract description 14
- 238000012364 cultivation method Methods 0.000 title claims abstract description 8
- 238000009395 breeding Methods 0.000 title description 6
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 40
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 40
- 239000002689 soil Substances 0.000 claims abstract description 21
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000000843 powder Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000000292 calcium oxide Substances 0.000 claims abstract description 4
- 235000012255 calcium oxide Nutrition 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000001301 oxygen Substances 0.000 claims abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 3
- 241000238631 Hexapoda Species 0.000 claims description 8
- 239000003337 fertilizer Substances 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 244000305267 Quercus macrolepis Species 0.000 claims description 3
- 241001233061 earthworms Species 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 3
- 239000000575 pesticide Substances 0.000 claims description 3
- 238000013138 pruning Methods 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 239000010865 sewage Substances 0.000 claims description 3
- 239000008239 natural water Substances 0.000 claims description 2
- 238000004904 shortening Methods 0.000 claims description 2
- 238000009966 trimming Methods 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 abstract description 3
- 238000004064 recycling Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 6
- 241000361919 Metaphire sieboldi Species 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000269350 Anura Species 0.000 description 1
- 241001489091 Ganoderma sinense Species 0.000 description 1
- 241001646828 Platostoma chinense Species 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mushroom Cultivation (AREA)
- Fertilizers (AREA)
Abstract
A complementary symbiotic three-dimensional cultivation and cultivation method for lucid ganoderma and wood frogs is characterized by comprising the following steps: the selected land section is pure natural and pollution-free, no residue is left in the land section, the soil quality is not over-alkaline, the soft sandy soil land section is deeply loosened by 40 centimeters in the first year, the land section is leveled, and the ganoderma lucidum frog shed can be built after 6 months of sunshine. The area is not too large, the area of the greenhouse is 200 flat, the length is 25 meters, the width is 8 meters, the height is 1.9 meters, the area is suitable for the complementary symbiotic growth environment of the ganoderma lucidum and wood frogs, the greenhouse is suitable for recycling the abalone powder, the wood frogs are breathed by skin, the area of the greenhouse is also suitable for the wood frogs, the ganoderma lucidum is ventilated, the wood frogs absorb oxygen and perform complementary symbiosis, and after the greenhouse is built, the sterilization is carried out by using quicklime water; and (3) selecting ganoderma lucidum mycelia, wherein ganoderma lucidum mycelia are ganoderma lucidum.
Description
Technical Field
The invention relates to a complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs.
Background
The wood frog is increasingly paid attention to by people due to the specific medicinal value and nutritive value thereof, and becomes the most economic one of frogs. Among the wood frogs produced in various regions, the wood frogs produced in Changbai mountain areas are the best products among the wood frogs due to large physical size, high oil yield, strong physique and high reproduction rate. The wood frog oil is rich in 18 amino acids, mineral substances and multiple vitamins, and the nutrient substances contained in the wood frog oil can meet the requirements of human bodies for various nutrient components, so that the wood frog oil has extremely high nutrition and nourishing effects, and the unique nutritional effect and medicinal value of the wood frog per se are continuously recognized by people. Therefore, health products using wood frogs as main raw materials are continuously developed and produced, resulting in the continuous increase of wood frog prices. But the number of wild wood frogs is limited. And the wild wood frog resources are endangered by the extinct edge due to continuous destructive damage, thereby seriously restricting the development of the wood frog processing industry. Therefore, industrialized and large-scale wood frog cultivation is the best way to solve the contradiction.
Ganoderma lucidum is also called Ganoderma lucidum, Mesona chinensis, Rumey, is a whole plant of Ganoderma lucidum or Ganoderma sinense of Polyporaceae, and because the organic matter content of the existing land is low, the Ganoderma lucidum is easy to die naturally in the second year by the commonly used Ganoderma lucidum planting method. Therefore, the problem is solved by complementary symbiosis of the ganoderma lucidum and the wood frogs.
Disclosure of Invention
Based on the defects, the invention provides a complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs. Solves the problem of continuous planting of the ganoderma lucidum, improves the planting soil, enables the ganoderma lucidum and the wood frog to be in complementary symbiosis, improves the land utilization rate, reduces the cost, greatly improves the benefit of a greenhouse, accelerates the fattening of the wood frog and shortens the finished product period.
The technology adopted by the invention is as follows: a complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs comprises the following steps:
(1) selecting natural sandy soil, requiring no pesticide and fertilizer residue, making soil not to be over-alkaline, making deep soil loosening by 40 cm in advance, leveling, making several months of sunshine, disinfecting and sterilizing with quicklime water to form symbiotic cultivation shed room; the area of the greenhouse is 200 square meters, the length is 25 meters, the width is 8 meters, and the height is 1.9 meters; ditches are arranged around the inner edge of the shed, pollution-free water is placed in the ditches, and sewage is discharged in an up-and-down circulation manner, so that the purpose of purification at any time is achieved;
(2) selecting Ganoderma lucidum as Ganoderma lucidum mycelium, culturing and inoculating the mycelium, and culturing in a culture shed for 45-60 days; cutting the soaked oak leaves into 12 cm long, binding to form a culture body with a diameter of 20-30 cm, transplanting the cultured ganoderma lucidum mycelia on the culture body, putting the culture body into the symbiotic culture shed room, covering the symbiotic culture shed room with fine sand of 1-1.5 cm per 1.4 square meters, and covering the shed for shading; the temperature of the constant temperature environment for culture is 28 ℃, and the humidity is 80-90%;
(3) pruning when the ganoderma lucidum is 2-3 cm, putting the wood frog into the wood frog, putting 70 wood frogs per square meter, weighing 15-25 g, keeping the surface temperature at 25-28 ℃, keeping the air temperature at 28-30 ℃ and keeping the humidity at 80-90%;
(4) growing in the dark, feeding living insect feed including fly maggot and earthworm at 8 am every day, averagely 5-10 mg/day for each wood frog, growing for 135-; and (4) manually picking the mature ganoderma lucidum.
The invention also has the following technical characteristics: and (2) placing sterilized and fermented cow dung with ammonia removed under the ganoderma lucidum cultivation body to serve as ganoderma lucidum base fertilizer, and simultaneously placing earthworm eggs into the base fertilizer to enable earthworms to naturally generate in soil.
Because the field of the artificially planted ganoderma lucidum is flat and the plant spacing is regular, the fall of the leaves is butted with the wood frog habit. When the wood frog habitat is moisturized, the effective moisturizing is naturally carried out on the vegetation crops of the wood frog habitat, two purposes are achieved at one time, multiple effects are achieved at one time, and the advantages are achieved at one place. Because the glossy ganoderma leaves are full, the water content is sufficient, the leaves are moderate in cooling and wetting and the oxygen permeable molecules are high. Therefore, the Chinese wood frog inhabits and grows under the ganoderma lucidum, the Chinese wood frog is basically in the environment with high ground gas and shade moisture in the land inhabitation process, and can contact with ganoderma lucidum leaves with more moisture for a long time, and the habit that the Chinese wood frog absorbs moisture by the skin and the ideal land-standing ecological conditions required by the Chinese wood frog are naturally butted. Various insects in the greenhouse for planting the lucid ganoderma are many and like to fall on lucid ganoderma leaves, and the insects are obvious particularly in rainy days, high temperature and windy days. The method naturally enables the insects and the wood frogs to be copolymerized in one room, so that the wood frogs can be preyed on the bait (the insects) in a short distance, the consumption of the body energy of the wood frogs in the bait feeding process is reduced, the fattening of the wood frogs is accelerated, and the finished product period is shortened. The Chinese wood frog is intercropped under the ganoderma lucidum, the most direct and obvious effect is that the Chinese wood frog is trained by the abalone powder, the medical value of the Chinese wood frog is improved, the land utilization rate is further improved, the content of organic matters in soil is improved, and the cost is reduced.
Detailed Description
The invention is further illustrated by the following examples:
example 1
A complementary symbiotic three-dimensional cultivation and breeding method for lucid ganoderma and wood frogs comprises the following steps:
(1) the position is selected from 43-45 degrees of north latitude, 124-127 degrees of east longitude, four seasons, rain and heat in the same season, more concentrated rainfall and moderate dryness and wetness. Is the most ideal living environment of the Chinese wood frogs. The air in the ganoderma lucidum shed is naturally aerobic, moist and slightly cloudy. Selecting natural sandy soil sections with the temperature difference of about 15 ℃ in the morning and evening and the surface temperature not exceeding 28-30 ℃, requiring no pesticide and fertilizer residues and no over-alkalinity of soil, deeply loosening the soil by 40 centimeters in advance, leveling, and building a symbiotic culture shed room after sterilization by quicklime water after several months of sunshine; the area of the greenhouse is 200 square meters, the length of the greenhouse is 25 meters, the width of the greenhouse is 8 meters, the height of the greenhouse is 1.9 meters, and the area is suitable for a complementary symbiotic growth environment of the ganoderma and the wood frogs and is suitable for the propagation of ganoderma spore powder; the inner edge of the shed is trimmed into a wood frog trimming pool (namely a ditch) with the depth of 45 cm, the width of 80 cm and the length of 25 m, pollution-free and purely natural water with the depth of 30 cm is filled, and circulating sewage is drained up and down to achieve the purpose of purifying the pool at any time.
(2) Selecting Ganoderma lucidum as Ganoderma lucidum mycelium, culturing and inoculating the mycelium, and culturing in a culture shed for 45-60 days; then cutting the sun-dried small-leaf oak tree into 12 cm long, binding into a culture body with the diameter of 20-30 cm, transplanting the lucid ganoderma on the culture body, putting the lucid ganoderma into the symbiotic cultivation shed room, covering the symbiotic cultivation shed room with fine sand of 1-1.5 cm per 1.4 square meters, and covering the shed for shading; the temperature of the constant temperature environment for culture is 28 ℃, and the humidity is 80-90%; the sterilized and fermented cow dung without ammonia gas is put under the ganoderma lucidum cultivation body to be used as ganoderma lucidum base fertilizer for cultivation, and simultaneously earthworm egg masses are put into the base fertilizer to naturally generate earthworms in soil, so that the effects of wood frog bait and soil loosening are achieved, the excrement of Chinese wood frogs is naturally absorbed by the soil, the soil is improved, the plant diseases and insect pests are reduced, the yield and the quality of the ganoderma lucidum are improved, and the cost is reduced, so that the effect is obvious.
(3) After the ganoderma lucidum is broken by 2-3 centimeters, pruning, putting wood frogs in 70 pieces per square meter, wherein the weight of each wood frog is 15-25 grams, the surface temperature is 25-28 ℃, the air temperature is 28-30 ℃, and the humidity is 80-90%;
(4) growing in dark light, feeding living insect feed including fly maggot and earthworm at 8 am every day, feeding each wood frog at an average of 5-10 mg/day, growing for 135-day plus 150 days, accelerating the fattening of wood frog, and shortening the final product period. The produced ganoderma lucidum mycelia are white, the pileus are reddish brown, the diameter of the pileus can reach 15-18 centimeters, the thickness of the pileus can reach about 1.5 centimeters, the length of the stipe can reach 15-20 centimeters, the thickness diameter of the stipe can reach 1.5-2 centimeters, the stipe is hard, the temperature resistance of the mycelia can be expanded to 10-27 ℃, the environment temperature suitable for growth can be 25-30 ℃, the yield of ganoderma lucidum and spore powder is greatly improved, the content of trace elements is greatly increased, the ganoderma lucidum and spore powder has good medicinal health care value and ornamental value, and the planting soil is improved.
Soil agrochemical sample detection table
Detection unit: and (3) a soil and fertilizer station of the Dulbert Mongolian autonomous county agricultural technology promotion center.
Claims (1)
1. A complementary symbiotic three-dimensional cultivation and cultivation method for lucid ganoderma and wood frogs is characterized by comprising the following steps:
(1) selecting the positions of 43-45 degrees of north latitude, 124-127 degrees of east longitude, four seasons, rain and heat in the same season, more concentrated rainfall, moderate dryness and humidity, natural oxygen and humidity ventilation of air in the lucid ganoderma greenhouse, slight shade, 15 ℃ of temperature difference between morning and evening, no more than 30 ℃ of surface temperature, selecting natural sandy soil sections, requiring no pesticide and fertilizer residues, no over-alkalinity of soil, deep loosening of soil by 40 centimeters in advance, leveling, sunshine for several months, and constructing a symbiotic culture greenhouse after disinfection and sterilization by quicklime water; the area of the greenhouse is 200 square meters, the length of the greenhouse is 25 meters, the width of the greenhouse is 8 meters, the height of the greenhouse is 1.9 meters, and the area is suitable for a complementary symbiotic growth environment of the ganoderma and the wood frogs and is suitable for the propagation of ganoderma spore powder; the inner edge of the shed is trimmed into a wood frog trimming pool with the depth of 45 cm, the width of 80 cm and the length of 25 m, pollution-free and purely natural water with the depth of 30 cm is filled, and circulating sewage is discharged from the upper part and the lower part, so that the aim of purifying the pool at any time is fulfilled;
(2) selecting Ganoderma lucidum as Ganoderma lucidum mycelium, culturing and inoculating the mycelium, and culturing in a culture shed for 45-60 days; then cutting the sun-dried small-leaf oak tree into 12 cm long, binding into a culture body with the diameter of 20-30 cm, transplanting the lucid ganoderma on the culture body, putting the lucid ganoderma into the symbiotic cultivation shed room, covering the symbiotic cultivation shed room with fine sand of 1-1.5 cm per 1.4 square meters, and covering the shed for shading; the temperature of the constant temperature environment for culture is 28 ℃, and the humidity is 80-90%; placing sterilized and fermented cow dung with ammonia gas removed under the ganoderma lucidum cultivation body;
(3) after the ganoderma lucidum is broken by 2-3 centimeters, pruning, putting wood frogs in 70 pieces per square meter, wherein the weight of each wood frog is 15-25 grams, the surface temperature is 25-28 ℃, the air temperature is 28-30 ℃, and the humidity is 80-90%;
(4) growing in dark light, feeding living insect feed comprising fly maggots and earthworms at 8 am every day, feeding each wood frog 5-10 mg/day averagely, growing for 135-150 days, accelerating the fattening of the wood frog, shortening the finished product period, wherein the produced ganoderma lucidum mycelia are white, the pileus is reddish brown, the diameter of the pileus reaches 15-18 cm, the thickness of the pileus reaches 1.5 cm, the length of the stipe reaches 15-20 cm, the thickness of the stipe reaches 1.5-2 cm, the stipe is hard, the temperature resistance of the mycelia is expanded to 10-27 ℃, and the environment temperature suitable for growth is 25-30 ℃.
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| CN107182734B (en) * | 2017-04-27 | 2020-06-02 | 江西九草铁皮石斛科技协同创新有限公司 | Dendrobium officinale and quasipaa spinosa intercropping method |
| CN107637385A (en) * | 2017-10-25 | 2018-01-30 | 四川三点水生物科技有限公司 | A kind of implantation methods of wild edible fungus |
| CN108834776A (en) * | 2018-07-16 | 2018-11-20 | 云南省农业科学院药用植物研究所 | A kind of implantation methods improving bigflower centranthera root tissue-cultured seedling transplanting survival rate |
| CN114027259B (en) * | 2021-12-03 | 2023-08-08 | 中国水产科学研究院黄海水产研究所 | Symbiotic cultivation method for Chinese wood frog and burdock |
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| KR100646132B1 (en) * | 2005-03-22 | 2006-11-14 | 이용철 | The rearing equipment for dybowski's brown frog and the rearing method |
| CN101663979A (en) * | 2009-10-16 | 2010-03-10 | 林贵夫 | Rice planting method for obtaining rice planted in field with frog |
| CN102124922A (en) * | 2010-01-18 | 2011-07-20 | 王景满 | High-nutrition multifunctional original ecosystem high-yield health-care rice and production method thereof |
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