KR20120121472A - CULTIVATING METHOD OF MUSHROOM INCLUDING Acanthopanax senticosus HOT WATER EXTRACT - Google Patents

Abstract

PURPOSE: A cultivation method of mushrooms using an Eleutherococcus senticosus hot water extract is provided to offer the abundant amount of active ingredients in Eleutherococcus senticosus to the mushrooms. CONSTITUTION: A cultivation method of mushrooms using an Eleutherococcus senticosus hot water extract comprises the following steps: cutting the stem, the root, and leaves of Eleutherococcus senticosus; mixing 100 parts of cut Eleutherococcus senticosus by weight with 200-400 parts of water by weight, and heating the mixture at 100-130 deg. C for 2-5 hours to obtain the Eleutherococcus senticosus hot water extract(S100); mixing the Eleutherococcus senticosus hot water extract with 2-3 times the amount of water to obtain a dilutant(S200); mixing 100 parts of sawdust by weight, 20-30 parts of rice bran by weight, and 0.5-5 parts of starch to obtain a mixed medium for the mushrooms(S300); adding 100-150 parts of Eleutherococcus senticosus hot water dilutant to 100 parts of mixed medium by weight(S400); and injecting mushroom spawns to the medium, and cultivating the mushrooms at 22-28 deg. C for 50-80 days(S500, S600). [Reference numerals] (AA) Mushroom containing active ingredients of Eleutherococcus senticosus; (S100) Producing hot-water extract; (S200) Diluting hot-water extract; (S300) Producing medium; (S400) Producing mixed medium using hot water extract; (S500) Injecting mushroom spawns to medium; (S600) Cultivating; (S700) Growing mushrooms

Description

Mushroom cultivation method using the hot water extract of thorny stem {CULTIVATING METHOD OF MUSHROOM INCLUDING Acanthopanax senticosus HOT WATER EXTRACT}

The present invention relates to a method of cultivating mushrooms using the hot water extract of thorny oocytes, and specifically, comprising the hot water extract of thorny oocytes with water in the culture medium for mushroom cultivation, and inoculating and culturing the mushroom bacteria in a mixed medium to contain the active ingredient of the thorny oocytes. To grow cultivated mushrooms.

Kashigagapi belongs to the genus Aralia and Ogalpi, better known as Siberian ginseng, and its native area is 40 to 50 degrees northeast of Far East Asia, the Russian Ussuri River, the Heilongjiang River in China, the North Sea of Japan, and the Alpine Region of Japan (over 1,100m) )to be.

The largest morphological difference distinguished from the genus Ogalpi is that the long needle-shaped spines, 0.5-0.8 cm in the stem, dense toward the ground. Flowers bloom in June, with stamens in the form of boots and shoes, and fruit ripens in September. Eludeside B, E, etc. are extracted from muscle and bark, and muscle strengthening, endurance improvement, fatigue recovery, tonic, gangjeong, neuralgia, stroke, diabetes, hypertension, hypotension, tenderness, insomnia, and rheumatism It is recognized as an excellent effect on health drinks and pharmaceutical raw materials have been developed and marketed. However, prickly pears are harvested and used as raw materials due to difficulties in breeding and cultivation. Prickly pears are often traded as smuggled goods in China and Russia, and in terms of efficacy, domestic products are superior to other countries. Vol. 1. 1994).

On the other hand, the active ingredient contained in the thorny ogapi which has the same efficacy as described above, the lignin glycosides (Acanthoside B, D), the water-soluble polysaccharide to increase the immunity, Chisanoside, Chilingin (Sylingin), There are coumarin glycoside components, and techniques for cultivating mushrooms or Cordyceps sinensis using the physiological properties of substances containing such active ingredients or contained in the bark have been proposed.

In this regard, the Republic of Korea Patent No. 0876820 is added in the cultivation of Cordyceps sinensis to provide a good quality Cordyceps spp. And fruiting bodies in a short time, and control the water content in induction of Cordyceps spp. This possible cultivation method has been proposed, but the cultivation method and characteristics of mushrooms and cordyceps are different and there are limitations that they cannot be directly applied to mushroom cultivation. In addition, the Republic of Korea Patent Publication No. 2003-0086749 also pulverized fruit body (leaves, stems, fruits, roots) of thorny Opiary callus cell culture or Acanthopanax spp, mixed in mushroom cultivation medium, and inoculated and incubated mushroom bacteria in the mixture Although a method for producing mushrooms containing an active ingredient of prickly pear is characterized, the cultivation process is not only complicated but also when the pulverized fruit body of prickly pear is used, the mushroom cannot be scattered or the active ingredient of prickly canopy is sufficiently contained. The problem still remained.

In addition, when inoculating the mushrooms using the culture medium, if the water supply is not sufficiently supplied to the medium, the mushroom spawn does not die or grow well, so the inconvenience of having to pay attention to the water supply must be resolved. The technology still remained.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems,

It is an object of the present invention to provide a mushroom cultivation method capable of cultivating high value-added mushrooms, which is functionally contained in mushrooms, which is sufficiently contained in mushrooms.

In addition, by using the thorny oak hot water extract, the active ingredient of thorny oocyte is sufficiently contained, there is another object of the present invention to provide a mushroom cultivation method using a simple yet effective thorny oak hot water extract compared to the existing cultivation method.

On the other hand, it is another object of the present invention to provide a mushroom cultivation method that is easy to control the water supply because the moisture resistance of the mushroom spawn becomes stronger when the thorny oak hot water extract is contained.

Mushroom cultivation method using the thorny oak hot water extract according to the present invention, the hot water extract manufacturing step of preparing a hot water extract of thorny ogapi by cutting the stem, leaves and roots of the thorny ogapi into water after heating; A hot water extract dilution step of preparing a hot water extract dilution by adding 2 to 3 times purified water to the hot water extract; A medium composition step of forming a mushroom culture medium by mixing 20 to 30 parts by weight of rice bran and 0.5 to 5 parts by weight of starch based on 100 parts by weight of sawdust; A step of producing a hot-water extract mixed medium for adding 100-150 parts by weight of the diluent of the hot-water extract with respect to 100 parts by weight of the mixed mushroom cultivation medium to form a hot-water extract mixed medium; Mushroom seed inoculation step of inoculating the seed of the mushroom in the hot water extract medium; A culture step of incubating the mushroom seed medium inoculated with hot water extract mixture medium for 22 days at 22 ° C. to 28 ° C. for 50 days to 80 days; And a mushroom growth step of growing the mushrooms by inducing the cultured mycelium to fruiting bodies for 10 to 16 days.

At this time, the mushroom is an edible mushroom, it is preferable to cultivate by culturing any one of oyster mushroom, enoki mushroom, shiitake mushroom, matsutake mushroom, willow mushroom, matsutake mushroom or mandala mushroom.

On the other hand, in the case of thorny ogapi, when cutting all the stems, leaves and roots, and then put it in water and heated, it is preferable to use the hot water extraction method because the active ingredients of the thorny ogapi are well dissolved in water and extracted. Thus, in the hot water extract manufacturing step, 200 to 400 parts by weight of water is added to 100 parts by weight of the visible shredded fibrils, and heated under pressure under a pressure of 100 to 130 ° C. for 2 to 5 hours to prepare hot water extracts of the shroud.

According to the present invention, since the active ingredient of prickly rot can be mixed into the medium better than the conventionally known thorny pulpy crushed powder directly, the cultivated high value-added mushroom having sufficient functionality is contained in the mushroom. The advantage is that you can.

In addition, by using the thorny oak hot water extract, the active ingredient of thorny oocyte is sufficiently contained, and there is another advantage that it is possible to provide a cultivation method using the simple yet effective thorny oak hot water extract compared to the existing cultivation method.

On the other hand, when prickly pear hot water extract is contained, the water resistance of mushroom spawn grown in the medium becomes stronger, which helps the mushroom spawn to grow and supplies the water better than other mushroom cultivation methods by allowing the mushroom to grow well even when water supply is insufficient. It is another advantage of the present invention that the control is easy.

1 is a process chart showing the entire process of the mushroom cultivation method using the oogapi hot water extract according to the present invention.
Figure 2 is a table showing the content of beta glucan by freezing and drying the shiitake mushroom prepared by the present invention and then powdered it.

Hereinafter, with reference to the accompanying drawings will be described an embodiment of the present invention;

1 is a process chart showing the entire process of the mushroom cultivation method using the oogapi hot water extract according to the present invention.

In the hot water extract manufacturing step (S100), the stems, leaves, and roots of the collected thorny oak are chopped and placed in water, and then applied with heat to sufficiently dissolve the active ingredients unique to the thorny oocyte. At this time, it is preferable that the weight ratio of thorny shredded crushed water and water before the hot water extraction is 1: 2-4, and after hot-water extracted, the hot-water extracted process is carried out so that the amount of thorny shredded crushed water and water remaining is 1: 1-1.5. It is preferable.

Prickly Ogapi is attracting attention because it is rich in many kinds of Ogapi glycosides, vitamins and minerals. On the other hand, scientific research on the pharmacological action of Ogapi began in the 1960s, and many active ingredients were identified in the 1970s, and then biological efficacy was proved. Their active ingredient is Acanthoside B, D, a lignin glycoside, and water-soluble polysaccharides that enhance immunity were isolated. The leaves have Chisanoside, which has a pharmacological function, and the root separates not only the Ogapi glycosides but also the Cylingin and coumarin glycosides. Since these ingredients are multiplying the functions of living organisms, they have an adaptive activity, which has a wide range of effects on curing, disease prevention, and autoimmune diseases. Since all of the active ingredients are water-soluble and are dissolved in water, supplying the active ingredients directly to the medium through hot water extraction may cause the mushroom body to contain a large amount of the active ingredients.

When the thorny oak hot water extract is prepared as described above, it is subjected to a dilution step (S200) of diluting it again with water. The thorny oak hot water extract is diluted by mixing water. Usually, water is diluted 2 to 4 times with respect to the extract to dilute the mushroom cultivation medium. Will be supplied.

In the medium composition step (S300), a conventional mushroom cultivation medium is prepared, and 20 to 30 parts by weight of rice bran and 0.5 to 5 parts by weight of starch are mixed with respect to 100 parts by weight of sawdust to form a culture medium for mushroom cultivation. Basically, it contains rice bran as a sawdust medium and nutrient and starch is used as supplementary nutritional ingredient. On the other hand, it is preferable to adjust the water content in the medium to 62 to 65%.

Thereafter, in the hot water extract mixed medium composition step (S400), mixed with 100 parts by weight of the mushroom cultivation medium for the mushrooms, the dilution of the thorny oak hot water extract in a ratio of 100 to 150 parts by weight, the thorny oak hot water extract is supplied to the mushroom cultivation containing sufficient moisture The medium will be formed. By supplying a diluent of the hot water extract instead of water, the active ingredients of prickly pears contained in the extract are sufficiently infiltrated into the medium.

On the other hand, after mixing the dilution of the hot water extract in the medium, it is important to maintain the surface temperature of the medium at a temperature of 18 ~ 20 ℃ to control the state of the medium so that the mushroom spawn is well seated in the medium. In addition, it is recommended to sterilize the medium before inoculation in order to prevent the penetration of other various bacteria and increase the inoculation rate of mushroom spawn.

In the mushroom spawn inoculation step (S500), the mushroom spawn is inoculated on the surface of the hot water extract mixture medium to be seated. Inoculate by grinding mushroom sawdust spawn in a hot water extract mixture medium stabilized at 18 ~ 20 ℃ to cover the hole and surface.

In the culturing step (S600), the inoculated mushroom spawn is cultivated. After inoculation, the medium is cultured at 18 to 20 ° C., a relative humidity of 65 to 70%, and dark conditions for about 25 to 30 days. In case of Pleurotus eryngii, incubate for 45-50 days including post-culture period.

Mushroom growth step (S700) is a step of growing into mushrooms using the cultured mushroom mycelium. After the culture is completed, the medium is cut to a depth of about 2cm to induce regeneration of hyphae to remove aging hyphae on the surface of the medium and to give mechanical stimulation for induction of feet. The dilution of the visible hot water extract through the nozzle is supplemented instead of water to induce culture. In case of supplying dilution solution of hot water extract, the water resistance of the culture spawn becomes stronger than that of water alone, so even if the surface of the medium is stopped to dry, it does not dry out and survives. This has the advantage of being possible.

When the entire culture vessel is infested with mushroom hyphae, open the lid and transfer the medium to the cultivator. After 15 ℃, humidity 85 ~ 90%, CO2 concentration 1500ppm or less, roughly 5 ~ 7 days at 200Lux, young mushrooms (fresh diameter) 1 to 2 mm) is generated. When young mushrooms occur, the grower's environment is grown after 4-5 days at 15 ℃, humidity 80 ~ 85%, CO2 concentration below 1000ppm, and roughness 200Lux. The method is a cultivation method of oyster mushroom and in case of Pleurotus eryngii the growth period takes about 15-18 days. On the other hand, the top mushrooms are grown after 7 to 10 days in the restraining work (even at 4? 5 ℃, 90% humidity, 1000 ppm or less of carbon dioxide concentration) for even growth after organic fertilization (5 ~ 8 ℃, 90% humidity, Harvesting is carried out under a carbon dioxide concentration of 1000 ppm or less).

Figure 2 is a table showing the content of beta glucan by freezing and drying the shiitake mushroom prepared by the present invention and then powdered it.

According to the results of the component analysis of the shiitake mushroom, the table contains 346.62mg of beta glucan per 1g of the shiitake mushroom freeze-dried powder, and the mushroom prepared by the present invention activates the immune function of human normal cells and activates cancer cells. It can be seen that it contains a large amount of beta glucan that has the function of inhibiting the proliferation and relapse of the blood, reducing blood sugar and cholesterol, improving lipid metabolism, and inhibiting the formation and accumulation of body fat.

Claims (3)

Hot water extract manufacturing step of preparing a hot water extract of thorn ogapi by cutting the stems, leaves and roots of thorny ogapi and mixing the water;
A hot water extract dilution step of preparing a hot water extract dilution by adding 2 to 3 times purified water to the hot water extract;
A medium composition step of forming a mushroom culture medium by mixing 20 to 30 parts by weight of rice bran and 0.5 to 5 parts by weight of starch based on 100 parts by weight of sawdust;
A hot water extract mixing medium for forming a hot water extract mixing medium by adding 100 to 150 parts by weight of the dilution hot water extract with respect to 100 parts by weight of the mushroom cultivation mixed medium;
Mushroom seed inoculation step of inoculating the seed of the mushroom in the hot water extract medium;
A culture step of incubating the mushroom seed medium inoculated with hot water extract mixture medium for 22 days at 22 ° C. to 28 ° C. for 50 days to 80 days; And
Mushroom cultivation method using the thorny oak hot water extract, characterized in that it comprises a mushroom growth step of growing the mushrooms by inducing the cultured mycelia to fruiting bodies for 10 to 16 days. In claim 1,
The mushroom is a mushroom cultivation method using the thorny oak hot water extract, characterized in that any one of the oyster mushroom, enoki mushroom, shiitake mushroom, matsutake mushroom, willow mushroom, mushroom mushroom or mandrel mushroom. In claim 1,
In the hot water extract manufacturing step, put the 200 ~ 400 parts by weight of water with respect to 100 parts by weight of the shredded oak bark and heated under pressure under the pressure of 100 ~ 130 ℃ for 2 to 5 hours Mushroom cultivation method using.

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