CN105766366A - Poria cocos planting method - Google Patents
Poria cocos planting method Download PDFInfo
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- CN105766366A CN105766366A CN201610033272.0A CN201610033272A CN105766366A CN 105766366 A CN105766366 A CN 105766366A CN 201610033272 A CN201610033272 A CN 201610033272A CN 105766366 A CN105766366 A CN 105766366A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a poria cocos planting method. The problem that existing planted poria cocos is low in survival rate is solved. The poria cocos planting method comprises the steps of poria cocos field selection, culture medium selection, poria cocos field treatment, culture medium treatment, strain selection, inoculation time selection, inoculation, survival checking, prevention and control of diseases and pests, earthing and gap filling, water draining, harvesting and processing for poria cocos obtaining. By the adoption of the technical scheme, the problems in the prior art can be well solved.
Description
Technical field
The present invention relates to plant planting field, be specifically related to the implantation methods of a kind of Poria.
Background technology
Poria is under the jurisdiction of Eumycota, Basidiomycetes, Aphyllophorales, Polyporaceae, and Poria belongs to.For the sclerotium of Polyporaceae sleeping hole platymiscium Poria, for rudimentary plant, it is colonize in the fungus on pinaster.Medicinal effects is dry sclerotia body;Its nature and flavor are sweet, light flat;There are the functions such as eliminating dampness by diuresis, spleen invigorating, mind calming;For phlegm retention, edema, dysuria, have loose bowels, cardiopalmus, dizziness.Cortex Sclerotii Poriae, inducing diuresis to remove edema, for diseases such as the wet edema of water.Poria is one of conventional Chinese crude drug of China, is also one of large outlet medical material.But owing to current Masson Pine resource is day by day exhausted, bacterium woods is particularly thorny, causes that Poria yield falls sharply, and purchasing price ramps.
Summary of the invention
Present invention aim to overcome that the defect of prior art, it is provided that the implantation methods of a kind of Poria, there is saving of labor, save time, be not take up ploughing, save that Masson Pine, quality be good, yield advantages of higher.
To achieve these goals, the technical scheme that the present invention takes is as follows:
A kind of implantation methods of Poria, including selecting Siberian cocklebur field, select cultivation matrix, process Siberian cocklebur field, process cultivation matrix, select good quality strain, inoculation timing, inoculation, one-tenth biopsy, insect pest preventing and controlling, ridging joint filling, draining, pick and process to obtain the process of Poria
The described hillside fields selecting Siberian cocklebur field to refer to that selection is sunny, the soil is porous is as Siberian cocklebur field, and the gradient as the hillside fields of Siberian cocklebur field is 10 ~ 30 °;Described Siberian cocklebur field is preferably and sits in the north facing the south;
Described selection cultivation matrix refers to that selection is felled then, bark does not fall off, the diameter loose container made of bamboo, wicker, ratten, etc. more than more than 10 is as cultivation matrix;
Described process Siberian cocklebur field refers to that the table soil within by 1m around the cultivation matrix of Siberian cocklebur field digs pine;The horizontal root making cultivation matrix exposes;
The process of described cultivation matrix refers to remove the peel the horizontal root of cultivation matrix stays muscle;This processing method accelerates distributing of culture substrate moisture;
Described selection good quality strain refers to, select cell age less than 45 days, mycelia is pure white, growing way is vigorous, bacterium wooden unit is faint yellow rotten shape, Poria strain without living contaminants, after being peelled off by its mycelia, when 25 DEG C, in 20 ~ 24 hours, its mycelia recovers is then good quality strain;
Described inoculation timing refers to when crackle occurs in cultivation matrix, can inoculate;
Described inoculation refers to good quality strain by the method grafting of high staking kind and/or the inoculation of the short container made of bamboo, wicker, ratten, etc. to cultivation matrix;
Described one-tenth biopsy goes to Siberian cocklebur field to observe after referring to inoculation 15 days early morning, and the earthing on all strains dries, it was shown that good quality strain survives;Earthing moistens, then strain does not survive, and will not survive strain and digs out, renewed vaccination;
Described insect pest preventing and controlling refers to that ZAILING field finds that Coptotermes formosanus Shtrari. exists, then be sprayed on Coptotermes formosanus Shtrari. with killing ant medicine, allow its band medicine return nest, it is achieved full nest is eliminated;
When described ridging joint filling refers to when the earthing on good quality strain is washed out by rainwater and knot Siberian cocklebur phase children Siberian cocklebur growth makes rapidly the be full of cracks of soil face, joint filling of all should earthing up;
Dredging gutter, Siberian cocklebur field when described draining refers to rainy season set, it is prevented that cultivation matrix is moisture overweight causes that mycelia stops growing;
Described processing is gathered and is referred to, first, during Poria maturation, gathers immediately at fine day, and covers tightly the Poria the gathered indoor lee Caulis et Folium Oryzae of heap placement or gunnysack so as to precipitate out moisture;Secondly, the Poria precipitating out moisture is spread after drying heap lid is brown to Poria epidermis shrinkage repeatedly out, and be cut into Poria block after peeling Poria epidermis;Finally, Poria block is dried.
Specifically, the method for described high staking kind refers to, first, saws out the breach of a long 12cm, deep 5 ~ 6cm in tree stump and the root junction, side of cultivation matrix, secondly, strain is put into above-mentioned breach build tight after, be cladded with fresh Folium Pini and tie up firm;Finally, earthing 4 ~ 6cm thereon so that earthing is steamed bread type, and covers the thin film of anti-rain.
Specifically, the method for described short container made of bamboo, wicker, ratten, etc. inoculation refers to, first, is removed the peel the side of the side root of cultivation matrix, exposes xylem, and by the Ramulus Pini that dries against above-mentioned xylem place;Then, strain is placed on the side root of peeling, builds with fresh Folium Pini or Masson Pine;Finally, earthing 4 ~ 6cm thereon, and cover the thin film of anti-rain.
The present invention compared with prior art, has the advantage that
The method of plantation Poria of the present invention makes Poria plantation survival rate bring up to 100%, adopts the pine container made of bamboo, wicker, ratten, etc. as cultivation matrix, and not extra land occupation has saved land resource greatly;The mode of inoculation and the process of Siberian cocklebur field, further ensure that effect of inoculation.
Detailed description of the invention
It is an object of the invention to overcome the defect of prior art, it is provided that the implantation methods of a kind of Poria.
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment
The implantation methods of a kind of Poria, including selecting Siberian cocklebur field, selects cultivation matrix, processes Siberian cocklebur field, processes cultivation matrix, selects strain, inoculation timing, inoculation, one-tenth biopsy, insect pest preventing and controlling, ridging joint filling, draining, pick and process to obtain the process of Poria.
The described hillside fields selecting Siberian cocklebur field to refer to that selection is sunny, the soil is porous is as Siberian cocklebur field, and the gradient as the hillside fields of Siberian cocklebur field is 10 ~ 30 °;Described Siberian cocklebur field is preferably and sits in the north facing the south;
Described selection cultivation matrix refers to that selection is felled then, bark does not fall off, Low grease, the diameter loose container made of bamboo, wicker, ratten, etc. more than more than 10 is as cultivation matrix;
Described process Siberian cocklebur field refers to that the table soil within by 1m around the cultivation matrix of Siberian cocklebur field digs pine;The horizontal root making cultivation matrix exposes;
The process of described cultivation matrix refers to remove the peel the horizontal root of cultivation matrix stays muscle;This processing method accelerates distributing of culture substrate moisture;
Described selection good quality strain refers to, select cell age less than 45 days, mycelia is pure white, growing way is vigorous, bacterium wooden unit is faint yellow rotten shape, Poria strain without living contaminants, after being peelled off by its mycelia, when 25 DEG C, in 20 ~ 24 hours, its mycelia recovers is then good quality strain;
Described inoculation timing refers to when crackle occurs in cultivation matrix, can inoculate;
Described inoculation refers to good quality strain by the method grafting of high staking kind and/or the inoculation of the short container made of bamboo, wicker, ratten, etc. to cultivation matrix;The method of described high staking kind refers to, first, saws out the breach of a long 12cm, deep 5 ~ 6cm in tree stump and the root junction, side of cultivation matrix, secondly, strain is put into above-mentioned breach build tight after, be cladded with fresh Folium Pini and tie up firm;Finally, more thereon earthing 4 ~ 6cm so that earthing is steamed bread type, and covers the thin film of anti-rain.The method of described short container made of bamboo, wicker, ratten, etc. inoculation refers to, first, is removed the peel the side of the side root of cultivation matrix, exposes xylem, and by the Ramulus Pini that dries against above-mentioned xylem;Then, strain is placed on Ramulus Pini and on the side root of peeling, builds with fresh Folium Pini or Masson Pine;Finally, earthing 4 ~ 6cm on Folium Pini or Masson Pine, and cover the thin film of anti-rain.
Described one-tenth biopsy goes to Siberian cocklebur field to observe after referring to inoculation 15 days early morning, and the earthing on all strains dries, it was shown that good quality strain survives;Earthing moistens, then strain does not survive, and will not survive strain and digs out, renewed vaccination;
Described insect pest preventing and controlling refers to that ZAILING field finds that Coptotermes formosanus Shtrari. exists, then be sprayed on Coptotermes formosanus Shtrari. with killing ant medicine, allow its band medicine return nest, it is achieved full nest is eliminated;
When described ridging joint filling refers to when the earthing on good quality strain is washed out by rainwater and knot Siberian cocklebur phase children Siberian cocklebur growth makes rapidly the be full of cracks of soil face, joint filling of all should earthing up.
Dredging gutter, Siberian cocklebur field when described draining refers to rainy season set, it is prevented that cultivation matrix is moisture overweight causes that mycelia stops growing;
Described processing is gathered and is referred to, first, during Poria maturation, gathers immediately at fine day, and covers tightly the Poria the gathered indoor lee Caulis et Folium Oryzae of heap placement or gunnysack so as to precipitate out moisture;Secondly, the Poria precipitating out moisture is spread after drying heap lid is brown to Poria epidermis shrinkage repeatedly out, and peel the Poria block that Poria epidermis rear cutout becomes specification to be 4*4*0.5cm;Finally, Poria block is dried.
Specifically, first good quality strain is cultivated before good quality strain is inoculated, comprises the following steps:
The Poria meat taken after good quality strain cleaning and sterilizing in the middle part of strain is put into culture medium, and puts it in the incubator that temperature is 20 ~ 25 DEG C, cultivate 7 ~ 10 days, obtain strain;Described culture medium include the peptone of 5 weight portions, the yeast extract powder of 2 weight portions, the glucose of 20 weight portions, 1 part dipotassium hydrogen phosphate, the magnesium sulfate of 0.5 weight portion, the agar of 10 weight portions, the distiller's dried grain of 15 weight portions, the Testa Tritici of 18 weight portions, the brown sugar of 1 weight portion, distilled water appropriate;The pH value of described culture medium is 6.2 ~ 6.6.
It is observed that employing the method for the invention, the survival rate of Poria reaches 100%.
Present disclosure is not limited to cited by embodiment, and the conversion of any equivalence that technical solution of the present invention is taked by those of ordinary skill in the art by reading description of the present invention, the claim being the present invention is contained.
Claims (3)
1. the implantation methods of a Poria, it is characterized in that, including selecting Siberian cocklebur field, select cultivation matrix, process Siberian cocklebur field, process cultivation matrix, select good quality strain, inoculation timing, inoculation, one-tenth biopsy, insect pest preventing and controlling, ridging joint filling, draining, pick and process to obtain the process of Poria
The described hillside fields selecting Siberian cocklebur field to refer to that selection is sunny, the soil is porous is as Siberian cocklebur field, and the gradient as the hillside fields of Siberian cocklebur field is 10 ~ 30 °;Described Siberian cocklebur field is preferably and sits in the north facing the south;
Described selection cultivation matrix refers to that selection is felled then, bark does not fall off, the diameter loose container made of bamboo, wicker, ratten, etc. more than more than 10 is as cultivation matrix;
Described process Siberian cocklebur field refers to that the table soil within by 1m around the cultivation matrix of Siberian cocklebur field digs pine;The horizontal root making cultivation matrix exposes;
The process of described cultivation matrix refers to remove the peel the horizontal root of cultivation matrix stays muscle;
Described selection good quality strain refer to select cell age less than 45 days, mycelia is pure white, growing way is vigorous, bacterium wooden unit is faint yellow rotten shape, Poria strain without living contaminants, after its mycelia is peelled off by selection especially, when 25 DEG C, the good quality strain that in 20 ~ 24 hours, its mycelia recovers;
Described inoculation timing refers to when crackle occurs in cultivation matrix, can inoculate;
Described inoculation refers to good quality strain by the method grafting of high staking kind and/or the inoculation of the short container made of bamboo, wicker, ratten, etc. to cultivation matrix;
Described one-tenth biopsy goes to Siberian cocklebur field to observe after referring to inoculation 15 days early morning, and the earthing on all strains dries, it was shown that good quality strain survives;Earthing moistens, then strain does not survive, and will not survive strain and digs out, renewed vaccination;
Described insect pest preventing and controlling refers to that ZAILING field finds that Coptotermes formosanus Shtrari. exists, then be sprayed on Coptotermes formosanus Shtrari. with killing ant medicine, allow its band medicine return nest, it is achieved full nest is eliminated;
When described ridging joint filling refers to when the earthing on good quality strain is washed out by rainwater and knot Siberian cocklebur phase children Siberian cocklebur growth makes rapidly the be full of cracks of soil face, joint filling of all should earthing up;
Dredging gutter, Siberian cocklebur field when described draining refers to rainy season set, it is prevented that cultivation matrix is moisture overweight causes that mycelia stops growing;
Described processing is gathered and is referred to, first, during Poria maturation, gathers immediately at fine day, and covers tightly the Poria the gathered indoor lee Caulis et Folium Oryzae of heap placement or gunnysack so as to precipitate out moisture;Secondly, the Poria precipitating out moisture is spread after drying heap lid is brown to Poria epidermis shrinkage repeatedly out, and be cut into Poria block after peeling Poria epidermis;Finally, Poria block is dried.
2. the implantation methods of a kind of Poria according to claim 1, it is characterized in that, the method of described high staking kind refers to, first, tree stump at cultivation matrix joins, with side root, the breach to saw out a long 12cm, deep 5 ~ 6cm, secondly, strain is put into above-mentioned breach build tight after, be cladded with fresh Folium Pini and tie up firm;Finally, earthing 4 ~ 6cm thereon so that earthing is steamed bread type, and covers the thin film of anti-rain.
3. the implantation methods of a kind of Poria according to claim 1, it is characterised in that the method for described short container made of bamboo, wicker, ratten, etc. inoculation refers to, first, is removed the peel the side of the side root of cultivation matrix, exposes xylem, and by the Ramulus Pini that dries against above-mentioned xylem place;Then, strain is placed on the side root of peeling, builds with fresh Folium Pini or Masson Pine;Finally, earthing 4 ~ 6cm thereon, and cover the thin film of anti-rain.
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Cited By (6)
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CN106613329A (en) * | 2016-11-23 | 2017-05-10 | 王贵田 | Transplantation-free wolfiporia extensa planting method |
CN106613330A (en) * | 2016-11-23 | 2017-05-10 | 王贵田 | Planting method for poriacocos |
CN110574638A (en) * | 2019-10-29 | 2019-12-17 | 遵义市林业科学研究所 | a device that is used for field inoculation of delicious boletus |
CN111684938A (en) * | 2020-05-26 | 2020-09-22 | 王业根 | Method for improving grafting survival rate of fresh poria cocos blocks |
CN111771606A (en) * | 2019-04-05 | 2020-10-16 | 杜方平 | Method for planting poria with hostwood in bark-free pine |
CN111869505A (en) * | 2020-08-12 | 2020-11-03 | 湖南美园生物科技发展有限公司 | Pine tuckahoe planting method |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106613329A (en) * | 2016-11-23 | 2017-05-10 | 王贵田 | Transplantation-free wolfiporia extensa planting method |
CN106613330A (en) * | 2016-11-23 | 2017-05-10 | 王贵田 | Planting method for poriacocos |
CN111771606A (en) * | 2019-04-05 | 2020-10-16 | 杜方平 | Method for planting poria with hostwood in bark-free pine |
CN110574638A (en) * | 2019-10-29 | 2019-12-17 | 遵义市林业科学研究所 | a device that is used for field inoculation of delicious boletus |
CN110574638B (en) * | 2019-10-29 | 2021-10-19 | 遵义市林业科学研究所 | A device that is used for field inoculation of delicious boletus |
CN111684938A (en) * | 2020-05-26 | 2020-09-22 | 王业根 | Method for improving grafting survival rate of fresh poria cocos blocks |
CN111869505A (en) * | 2020-08-12 | 2020-11-03 | 湖南美园生物科技发展有限公司 | Pine tuckahoe planting method |
CN111869505B (en) * | 2020-08-12 | 2022-05-17 | 湖南美园生物科技发展有限公司 | Pine tuckahoe planting method |
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