CN103210786A - Method for planting tuckahoe by mother tuckahoe fixed-point core forming - Google Patents

Method for planting tuckahoe by mother tuckahoe fixed-point core forming Download PDF

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Publication number
CN103210786A
CN103210786A CN2012103646384A CN201210364638A CN103210786A CN 103210786 A CN103210786 A CN 103210786A CN 2012103646384 A CN2012103646384 A CN 2012103646384A CN 201210364638 A CN201210364638 A CN 201210364638A CN 103210786 A CN103210786 A CN 103210786A
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China
Prior art keywords
siberian cocklebur
poria cocos
tuberculosis
tuckahoe
fixed point
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CN2012103646384A
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Chinese (zh)
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江庆伍
张立明
郦旻
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ANHUI JINZHAI QIAOKANG PHARMACEUTICAL Co Ltd
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ANHUI JINZHAI QIAOKANG PHARMACEUTICAL Co Ltd
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Priority to CN2012103646384A priority Critical patent/CN103210786A/en
Publication of CN103210786A publication Critical patent/CN103210786A/en
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Abstract

The invention discloses a method for planting tuckahoe by mother tuckahoe fixed-point core forming. The method is characterized in that after a material cylinder is put into a cellar and is inoculated for one month, hypha fully grows in the material cylinder to form the sclerotium phase, soil covered at the bottom end of the material cylinder is removed, 50 to 100g of mother tuckahoe which is bred in advance is tightly attached at the bottom end of the material cylinder, and then the soil is covered to continuously culture. The method for planting the tuckahoe by the mother tuckahoe fixed-point core forming has the advantages that the yield is improved by more than 30% in comparison with the traditional method; the fruiting body is thin-skinned and flesh, the texture is close, the tuckahoe monomers which are fruited in the single cellar are single and uniform, the processing finished rate is high, and the quality is high; and the core forming position is fixed and single and is suitable for the management and harvesting in the growth period.

Description

A kind of method of female Siberian cocklebur fixed point tuberculosis plantation Poria cocos
Technical field
The invention belongs to the breeding method of microorganism, relate in particular to the method that Poria cocos is cultivated in the plantation of a kind of female Siberian cocklebur fixed point tuberculosis.
Background technology
Poria cocos is a kind of saprophytic bacteria, and mycelia obtains nutrition from withered pine stump, pine.Poria cocos is different with edible mushroom, and it is made up of mycelium, sclerotium, fruit body 3 parts.Medicinal part is a sclerotium, and it is the traditional rare traditional Chinese medicine of China, is described as " silver " of Chinese conventional medicament.Poria cocos system large traditional Chinese medicine commonly used, edible also can be used as medicine.According to the data show that national natural resources of Chinese medicinal materials reconnaissance information provides, this product annual turnover reaches 1,000 ten thousand kilograms, and the huge market demand is profitable.
The Poria cocos artificial culture is in the history in existing more than 1500 year of China.Start from the Northern Dynasties the earliest, but the sclerotium that produces very little, quality is also poor.Arrived Song dynasty's culture technique and obviously improved, started from " meat draws " kind Siberian cocklebur, output nuclear quality all is much improved." meat draws " plants the Siberian cocklebur technology and continues to use to more present old Poria cocos producing regions always.Barrel cultivation is to be raw material with trees, is cut into the wood tube and cultivates.Also claim section wood to cut cultivation, concrete technology is as follows:
1, Poria cocos selected scenes: Poria cocos is preferably selected hillside to the south, and the gradient is with 15 °~35 ° angle the bests.Soil is best with sandy loam, and clay and grit soil should not utilize.The all places of cultivating Poria cocos of continuous cropping are must guard against in the Poria cocos cultivation, can not plant Poria cocos again in 3 years.After choosing, the Siberian cocklebur field cultivates ground earlier, cleaning weeds, tree root and stone; To hold the drain ditch successfully around the place, prevent to wash away and ponding; The powder that will lime around in the field, control termites harm; Inoculate preceding 10 days and will plough once, smash earth.
2, barrel requires: various pine trees such as masson pine, Japanese red pine, Korean pine, black pine etc. all can be used for cultivating Poria cocos.Other weedtrees also can, but not as the pine high yield.Tree diameters is to be advisable more than 9 centimetres.Generally get the raw materials ready in 11~December, inoculate 1 year 3~April.Tree is treated after cutting dried slightly, is sawn into the Duan Mu about 60 centimetres again.The strip of paper used for sealing of pruning after blocking.The first wide about 3 centimetres bark together of vertically pruning on one side of Duan Mu of peeling method, the degree of depth is advisable to see xylem, every 3 centimetres of (keeping 3 centimetres bark) barks of pruning again one, width is identical with first road with the degree of depth, promptly stays skin portion to equate with truncated section then.The purpose of peeling is that quickening trunk lactones overflows and drying.Also help mycelia the place's of peeling field planting block Duan Mu after peeling be placed on ventilation, on the sunny side, dry place, intersect anyhow and stack with " well " font, allow its drying.Pile highly about 1.5 meters, cover bark, cogongrass etc. above with anti-rain.General composting had a lot of tiny wrinkles all over the body until barrel about 100 days, and manual firing goes out the clear and melodious sound of " kowtow, kowtow ", and this moment, water content was about about 25% for suitable.
3, bacterial classification is quoted: the bacterial classification of Poria cocos is commonly called as and is " Siberian cocklebur is drawn ".Can be divided into that meat draws that (sclerotium section for kind), wood draw (the Duan Muwei kind that refers to cover with mycelia) and pure bacterium is drawn (refer to separate purification bacterial classification) 3 kinds according to bacterial classification source.
(1) meat draws: the kind Siberian cocklebur in the most handy generation or two generations, medium stature, stout and strong, ripe, be no more than 15 days after digging, new fresh hide is thin to be pale red, meat white, juice is many, without putrefaction is rotten.
(2) wood draws: allly cover with dense Poria cocos mycelia, be golden yellow, do not tie the Duan Mu of Siberian cocklebur as yet, all can be sawn into fritter, draw as wood.
(3) pure bacterium is drawn: the production of hybrid seeds according to a conventional method, all mycelia are covered with bottle, the pure white dense stalwartness of mycelia, no living contaminants and are breeding.All length has assorted bacterium or the mycelia bacterial classification of flavescence or brown, all can not be used for inoculation.Cell age is generally about 20 days better.
4, dig the cellar for storing things inoculation: select continuous sunny gas, take limit Wa Jiao, limit blanking inoculation.On ready Siberian cocklebur field, suitable slope scrapes out and is about 1 meter, the dark and wide cellar for storing things that is about 50 centimetres.About 20 centimetres of the distances at every cellar for storing things laterally reach and vertically are separated by number cellar for storing things (deciding on the gradient) and excavate 40 centimetres of drain ditchs that the left and right sides is wide.Then the dried barrel of foot is moved in the cellar for storing things, a cellar for storing things is put 3 or 5, decides on the size of barrel.About 25 kilograms of every cellar for storing things consumptions can be inoculated after blanking is intact.According to bacterium draw, meat draws, wood draws etc., and dissimilar Siberian cockleburs is drawn, and adopts corresponding inoculation method.
5, the flat cellar for storing things of earthing: the inoculation back is earthing in time, is incubated, preserves moisture, and mycelia normal growth under dark condition is grown, and helps the formation of sclerotium.Thickness of earth covering can be controlled in 4~6 centimetres, and is tightly not loose.But above cover film 3~5 days, infiltrate when preventing to rain and cause mashed Siberian cocklebur.
6, knot Siberian cocklebur management: Poria cocos is avoided wet, and the rainy day is wanted timely ditch cleaning draining, is strictly on guard against that rainwater enters the cellar for storing things.The weeds of Siberian cocklebur field and cellar for storing things face will in time be removed, to prevent the insect pest that spreads disease.Inoculate back about 10 days, push the inspection of inoculation place sandy soil aside, if the growth of no mycelia should be taken out old kind, other cuts new mouthful and reseeds.Inoculate after 20 days, mycelia extension situation is checked in the side that excavates barrel, should extend 15~30 centimetres usually.When mycelia extends to the lower end of barrel after 2 months, should excavate the lower end and check, if the lower end mycelia has sand shape phenomenon explanation well-grown; If mycelia rests on the stage casing, and mycelia is discontinuous, is commonly called as " skips " and illustrates that then growth is undesirable.Poria cocos inoculation usually began to form sclerotium after 2~3 months in the cellar for storing things, be commonly called as " knot Siberian cocklebur ".This moment, the soil surface cracking crackle of establishing an army should in time cover crackle with soil, in case Poria cocos emits unearthed face.
Poria cocos barrel cultivation process assorted bacterium such as Trichoderma viride, mould, Mucor can occur and infect.Prevent and treat method: before inoculation, ted the Siberian cocklebur field, utilize the UV sunlight sterilization; It is dark excessively that barrel can not bury ground; Cause that bacterial classification will strict quality.If find soft rotten symptom, should in time reject and burn or turn over and bury; Take to dig nest, the dispenser of ant road when finding white ant; To note wild boar arch cellar for storing things and people, animal trample in addition.
Be that traditional method is to adopt barrel to educate the Siberian cocklebur technology, the step of comprise that bacterial classification is made, cellar for storing things, inoculation, ridging, time spent in spring gathering under the autumn barrel, but conventional method is not owing to know the position of Poria cocos tuberculosis, so in fact be difficult to accomplish ridging operation regularly vegetative period, will cause the quick growth period of Poria cocos like this, Poria cocos expands the native explosion of table, after explosion place is subjected to solar radiation, suppress the growth of seed of Poria cocos entity, thereby influence the output of Poria cocos monomer.And single cellar for storing things knot seed of Poria cocos physical quantities is many, and monomer weight is light, at the bottom of the processed finished products rate, and bad, the tuberculosis position fixedly is not easy to growing season management and gathers.
Therefore, need a kind of fixed point tuberculosis planting technology, the back is regular by planting, the fixed point ridging, and tamps this soil, strengthens final-period management effectively, improves the output of Poria cocos monomer, is convenient to gathering and processing of later stage, improves the Poria cocos quality.The present inventor found through experiments, be about to form sclerotium period at Poria mycelium, allow female Siberian cocklebur closely paste mutually with mycelium, the human intervention sclerotium forms point, makes its process of growth directly stride across young tender sclerotium and forms the stage, thereby reach the purpose of fixed point tuberculosis, in case and this female Siberian cocklebur sclerotium successful introduction, will form " nuclear superiority early ", form the actual effect of tuberculosis, the big fruit of knot early, thereby finished the present invention.
Summary of the invention
The method that the object of the present invention is to provide the plantation of a kind of female Siberian cocklebur fixed point tuberculosis to cultivate Poria cocos, the more traditional method of Poria cocos output of this method plantation improves more than 30%, and the thin meat of fruit body skin is thick, quality is tight, single cellar for storing things knot Poria cocos individuality is single, even, processed finished products rate height, quality is good, tuberculosis fixed-site, single and be easy to growing season management and gather.
To achieve these goals, the present invention adopts following technical scheme:
A kind of method of female Siberian cocklebur fixed point tuberculosis plantation Poria cocos, adopt barrel to educate the Siberian cocklebur technology, after comprising that bacterial classification making, siccative tube are stored down, the step of gathering is waited in inoculation, ridging, it is characterized in that cellar for storing things, inoculation back cover with barrel up to mycelia and be about to form the sclerotium stage down from bacterial classification making, siccative tube, push barrel bottom earthing aside, the female Siberian cocklebur of the 50~100g that cultivates in advance is close to the barrel bottom, earthing continues to cultivate then.
Described female Siberian cocklebur requires to grow intact, bright and clean, tenderly white, no damage by disease and insect.Especially, described female Siberian cocklebur is for getting according to the conventional method plantation and in the Poria cocos stripping and slicing that gather spring in this year autumn last one year.
Described siccative tube is 15~35: 1 with the quality proportioning of inoculation bacterial classification; Preferably, described siccative tube is 20~30: 1 with the quality proportioning of inoculation bacterial classification; More preferably, described siccative tube is 25: 1 with the quality proportioning of inoculation bacterial classification.
The method of female Siberian cocklebur fixed point tuberculosis plantation Poria cocos of the present invention is because of tuberculosis fixed-site (all being grown in the barrel bottom); Every cellar for storing things seed of Poria cocos physical quantities is single, thus can accomplish that the plantation back is regular, the fixed point ridging, and tamp this soil.In fact new method has changed the growing environment of seed of Poria cocos entity: because of the airtight seed of Poria cocos entity growing environment that tension force is arranged of repeatedly earthing up, the compacting program is achieved; And traditional cultivation method do not have the ridging program or because of the ridging place not to the environment of soft, the native explosion of table that forms.
The outstanding feature of the method for female Siberian cocklebur fixed point tuberculosis plantation Poria cocos of the present invention is exactly that the more traditional method of output improves more than 30%, and the thin meat of fruit body skin is thick, quality is tight, single cellar for storing things knot Poria cocos individuality is single, even, processed finished products rate height, quality is good, tuberculosis fixed-site, single and be easy to growing season management and gather.
Embodiment
Below in conjunction with specific embodiment the present invention is done further expansion explanation, but it is pointed out that the method for female Siberian cocklebur fixed point tuberculosis plantation Poria cocos of the present invention is not limited to this concrete method and proportioning.Obviously be understandable that for those skilled in the art,, also can be directly applied in these unspecified other similar method and proportionings even the following description content is not done any adjustment or correction.
A kind of method of female Siberian cocklebur fixed point tuberculosis plantation Poria cocos, adopt barrel to educate the Siberian cocklebur technology, after comprising that bacterial classification making, siccative tube are stored down, the step of gathering is waited in inoculation, ridging, it is characterized in that cellar for storing things, inoculation back cover with barrel up to about 1 month mycelia and be about to form the sclerotium stage down from bacterial classification making, siccative tube, push barrel bottom earthing aside, the female Siberian cocklebur of the 50~100g that cultivates in advance is close to the barrel bottom, earthing continues to cultivate then.
Be the breeding method of Poria cocos of the present invention than traditional fado a step that connects female Siberian cocklebur.Wherein, described female Siberian cocklebur requires to grow intact, bright and clean, tenderly white, no damage by disease and insect, should not put for a long time with digging with planting.Especially, female Siberian cocklebur is for getting according to the conventional method plantation and in the Poria cocos stripping and slicing that gather spring in this year autumn last one year.The cultivation flow process of female Siberian cocklebur is: gather → stripping and slicing → female Siberian cocklebur down by cellar for storing things → inoculation → ridging → time spent in spring for bacterial classification barrel in making → autumn.
The method mechanism of female Siberian cocklebur fixed point tuberculosis plantation Poria cocos of the present invention is: be about to form sclerotium period at Poria mycelium, allow female Siberian cocklebur closely paste mutually with mycelium, the human intervention sclerotium forms point, make its process of growth directly stride across young tender sclerotium and form the stage, thereby reach the purpose of fixed point tuberculosis, in case and this female Siberian cocklebur sclerotium successful introduction, will form " nuclear superiority early ", form the actual effect of tuberculosis, the big fruit of knot early.
Especially, the method of female Siberian cocklebur fixed point tuberculosis plantation Poria cocos of the present invention, the siccative tube need satisfy specific ratio with the quality proportioning of inoculation bacterial classification, suitable bacterial classification amount can make bacterial classification cover with barrel with suitable growth time, the mycelia that guarantees when mycelia is covered with and inoculate the position the earliest can ripe aging beginning tuberculosis, connects female Siberian cocklebur like this this moment in the bottom and just can reach the purpose that the fixed point statement of account is examined.For example, described siccative tube is 15~35: 1 with the quality proportioning of inoculation bacterial classification; Preferably, described siccative tube is 20~30: 1 with the quality proportioning of inoculation bacterial classification; More preferably, described siccative tube is 25: 1 with the quality proportioning of inoculation bacterial classification.For example, 12.5kg siccative socket joint kind 500g bacterial classification.
The method of female Siberian cocklebur fixed point tuberculosis plantation Poria cocos of the present invention only than traditional fado a step that connects female Siberian cocklebur, only need wait until under the siccative tube that the mycelia of cellar for storing things, inoculation bacterial classification covers with barrel and be about to form sclerotium in the time of the stage and connect female Siberian cocklebur, push barrel bottom earthing aside, the female Siberian cocklebur of the 50~100g that cultivates in advance is close to the barrel bottom, the earthing continuation is cultivated and is got final product then, other steps all can be with reference to prior art, does not add at this and gives unnecessary details.
Because of the method for female Siberian cocklebur fixed point tuberculosis plantation Poria cocos of the present invention because of the tuberculosis position all in the barrel bottom, every cellar for storing things seed of Poria cocos physical quantities is single, can accomplish that the plantation back is regular, the fixed point ridging, and tamp this soil, changed the growing environment of seed of Poria cocos entity, achieved airtight and the seed of Poria cocos entity growing environment of tension force has been arranged, make the more traditional method of Poria cocos output improve more than 30%, and the thin meat of fruit body skin is thick, quality is tight, single cellar for storing things knot Poria cocos individuality is single, even, processed finished products rate height, quality is good, tuberculosis fixed-site, single and be easy to growing season management and gather.
Although above the specific embodiment of the present invention has been given to describe in detail and explanation; but what should indicate is; we can carry out various equivalences to above-mentioned embodiment according to conception of the present invention and change and modification; when the function that it produced does not exceed spiritual that specification contains yet, all should be within protection scope of the present invention.

Claims (6)

1. the method for a female Siberian cocklebur fixed point tuberculosis plantation Poria cocos, adopt barrel to educate the Siberian cocklebur technology, after comprising that bacterial classification making, siccative tube are stored down, the step of gathering is waited in inoculation, ridging, it is characterized in that cellar for storing things, inoculation back cover with barrel up to mycelia and be about to form the sclerotium stage down from bacterial classification making, siccative tube, push barrel bottom earthing aside, the female Siberian cocklebur of the 50~100g that cultivates in advance is close to the barrel bottom, earthing continues to cultivate then.
2. the method for female Siberian cocklebur fixed point tuberculosis plantation Poria cocos according to claim 1 is characterized in that described female Siberian cocklebur requires to grow intact, bright and clean, tenderly white, no damage by disease and insect.
3. the method for female Siberian cocklebur fixed point tuberculosis plantation Poria cocos according to claim 2 is characterized in that described female Siberian cocklebur is for getting according to the conventional method plantation and in the Poria cocos stripping and slicing that gather spring in this year autumn last one year.
4. the method for female Siberian cocklebur fixed point tuberculosis plantation Poria cocos according to claim 1 is characterized in that the described siccative tube and the quality proportioning of inoculation bacterial classification are 15~35: 1.
5. the method for female Siberian cocklebur fixed point tuberculosis plantation Poria cocos according to claim 4 is characterized in that the described siccative tube and the quality proportioning of inoculation bacterial classification are 20~30: 1.
6. the method for female Siberian cocklebur fixed point tuberculosis plantation Poria cocos according to claim 5 is characterized in that the described siccative tube and the quality proportioning of inoculation bacterial classification are 25: 1.
CN2012103646384A 2012-09-27 2012-09-27 Method for planting tuckahoe by mother tuckahoe fixed-point core forming Pending CN103210786A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104381017A (en) * 2014-10-31 2015-03-04 颍上县永祥旱粮研究所 Shaping and directional cultivation method for Poria cocos
CN105766366A (en) * 2016-01-19 2016-07-20 巴中市通江银耳产业技术研究院 Poria cocos planting method
CN114503875A (en) * 2022-01-30 2022-05-17 陕西森盛菌业科技有限公司 Directional growth cultivation method for poria cocos
CN114716218A (en) * 2022-05-18 2022-07-08 浙江甚骅建设有限公司 Pervious concrete prepared based on building waste materials and preparation method thereof

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CN1451262A (en) * 2003-05-23 2003-10-29 王克勤 Poria attraction cultivating method

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Publication number Priority date Publication date Assignee Title
CN1451262A (en) * 2003-05-23 2003-10-29 王克勤 Poria attraction cultivating method

Non-Patent Citations (2)

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李苓等: "茯苓诱引栽培技术研究", 《中国现代中药》 *
王克勤等: "茯苓诱引栽培", 《2206海峡两岸暨CSNR全国第七届天然药物资源学术研讨会论文集》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104381017A (en) * 2014-10-31 2015-03-04 颍上县永祥旱粮研究所 Shaping and directional cultivation method for Poria cocos
CN105766366A (en) * 2016-01-19 2016-07-20 巴中市通江银耳产业技术研究院 Poria cocos planting method
CN114503875A (en) * 2022-01-30 2022-05-17 陕西森盛菌业科技有限公司 Directional growth cultivation method for poria cocos
CN114716218A (en) * 2022-05-18 2022-07-08 浙江甚骅建设有限公司 Pervious concrete prepared based on building waste materials and preparation method thereof

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Application publication date: 20130724