CN101524035B - Artificial culture method of fuscous dictyostelium boletes - Google Patents
Artificial culture method of fuscous dictyostelium boletes Download PDFInfo
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- CN101524035B CN101524035B CN2009100943902A CN200910094390A CN101524035B CN 101524035 B CN101524035 B CN 101524035B CN 2009100943902 A CN2009100943902 A CN 2009100943902A CN 200910094390 A CN200910094390 A CN 200910094390A CN 101524035 B CN101524035 B CN 101524035B
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- Mushroom Cultivation (AREA)
Abstract
The invention relates to a method for artificially culturing fuscous dictyostelium boletes in mushroom houses, greenhouses, field and nursery. The method is characterized in that firstly bolete rods are cultured, then (1) the bolete rods are earthed up to culture mushroom in mushroom houses and greenhouses, fuscous dictyostelium boletes substance can be grown and developed to mature after 10-15 days of earthing; (2) the bolete rods are debagged and earthed up to culture mushroom in mushroom houses and greenhouses, and fuscous dictyostelium boletes substance can be grown and developed to mature after 20-25 days of earthing; (3) solid bolete strains are inoculated at the roots of tropical cash crops and flower plants planted in fields to culture mushroom, and fuscous dictyostelium boletes substance can be grown and developed to mature after 30-60 days of inoculation; artificial culture of fuscous dictyostelium boletes and the industrialization production of the fuscous dictyostelium boletes can be fast realized. The culture method is simple and convenient, the culture period is short and the fruiting is regular and the yield is high.
Description
Technical field
The present invention relates to edible fungus culture technique field, especially dun net handle bolete in mushroom room, green house and field, the artificial cultivation method on garden ground.
Background technology
Dun net handle bolete (Phlebopus portentosus), describe the earliest and see Sri Lanka (Pegler 1986), present Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia, all there is distribution (Maria2007 in New Zealand, Bandala2004, Watling2001, Barbara1987, Heinemann1982).Be distributed in ground such as Yunnan, Guangxi and Hainan (good Mu etc. 1986,2006 in China; Yang Zhuliang and a surname solemn 2003), be a kind of delicious flavour, nutritive value is abundant, the delicious edible mushroom that liked by place of production consumer.
Most boletes are the mycorhiza edible mushroom.The process of many mycorhiza edible mushroom half manual simulations cultivation is quite long, needs 4～5 years, even just can finish (bend bright by the emperor himself etc. 2006) in 6～7 years.Boletus mycelia well-grown on the medium of sawdust and wheat composition is cultivated the mature sporophore (Ohta A etc. 2003) that produced the tool basidiospore in 90 days under the lucifuge condition under laboratory condition.That the edible cultivation of world's mycorhiza at present is the most successful is black truffle (Tuber melanosporum).In New Zealand, the black truffle artificial cultivation needs the 6-7 year (the bright grade 2002 of Tan's work) from the ferfas ascocarp results that have been established in plantation.State's endotrophic mycorrhiza edible fungus culturing research is later relatively.Delicious lactarius (Lactarius deliciosus) artificial cultivation, the mycorhiza seedling is synthetic to need 5～8 months, continues the original place cultivation or be transplanted to cultivate fruiting in the normal soil and need 2～3 years (Tan's work is bright etc. 2000) with the synthetic seedling of mycorhiza.Adopt synthetic suillus lutens (Suillus luteus) wet-land pine tree mycorhiza seedling dense planting to realize artificial cultivation in the exclosure through sterilization, the mycorhiza seedling is planted to the suillus lutens sporophore growth and is needed 12 months (Ying Guohua etc. 2007).In sum, only there is the mycorhiza edible mushroom of a few species can carry out half manual simulation cultivation and manual simulation's cultivation at present, or is in laboratory cultivation research state.Dun net handle bolete artificial cultivation does not appear in the newspapers.
Dun net handle bolete delicious flavour, nutritive value is abundant, and edible preferably and medical value are arranged, and is that a kind of guarantor preferably built food, liked by place of production consumer.The dun net handle bolete of Xiao Shouing is all picked up from wild self-sow in the market.Owing to be subjected to limiting output of climatic season condition limited.Add the protection of not paying attention to wild self-sow resource, artificial excessively the harvesting causes quantity fewer and feweri.
List of references:
[1]Pegler?DN，Agaric?Flora?of?Sri?Lanka.Kew?Bulletin?Additional?Series，1986，12：1-519.
[2]Barbara?P，Segedin.An?annotated?checklist?of?Agarics?and?Boleti?recorded?from?New?Zealand.NewZealand?Journal?of?Botany，1987，25(2)：185-215.
[3]Watling?R.The?relationships?and?possible?distributional?patterns?of?Boletes?in?South-East?Asia.Mycological?Research，2001，105(12)：1440-1448.
[4]Bandala?VM，Montoya?L，Jarvio?D.Two?interesting?records?of?Boletes?found?in?coffee?plantations?inEastern?Mexico.Persoonia，2004，18(3)；365-380.
[5]Maria?alice?neves，Marina?capelari.A?preliminary?checklist?of?Boletales?from?Brazil?and?notes?on?Boletalesspecimens?at?the?Instituto?de
(SP)Herbarium，
Paulo，SP，Brazil.Sitientibus?serie?cienciasbiologicas，2007，7(2)；163-169.
[6]Heinemann?P，Rammeloo?J.Observations?sur?le?genre?Phlebopus(Boletineae).Mycotaxon，1982，15：384-404.
[7] a surname is solemn. the research (continuing) of China's east Himalaya and adjacent area Boletales thereof. and Yunnan plant research, 1986,8 (1): 1-22.
[8] a surname is solemn, 2006. Chinese fungi will the 22nd volumes. Beijing: scientific publication, pp.1-215.
[9] Yang Zhuliang, a surname is solemn. the tropical relationship of SOUTHERN CHINA higher fungi. and Yunnan plant research, 2003,25 (2): 129-144.
[10] bright admire Zhong Chong high post Chen Yu .2006. mycorhiza edible mushroom and half artificial cultivation thereof of bow. Guangdong: Guangdong Science Press, pp.1-147.
[11]Ohta?A，Fujiwara?N.Fruit-body?production?of?an?ectomycorrhizal?fungus?in?genus?Boletusin?pure?culture.Mycoscience，2003，44(4)：295-30.
[12] Tan Zhuming, Fu Shaochun, Liu Xiaohong. New Zealand's edible mycorrhizal fungi research and development present situation and enlightenment. Hunan forestry science and technology, 2002,29 (3): 72-74.
[13] Tan Zhuming. delicious lactarius mother culture media and artificial cultivation method, People's Republic of China's patent, ZL98112527.1.
[14] Ying Guohua, Lv Mingliang, Li Lingli. brown yellow suillusbovinus artificial cultivation method, People's Republic of China's patent, the number of applying for a patent, 200710164731.X.
Summary of the invention
The purpose of this invention is to provide a kind of artificial culture method of fuscous dictyostelium boletes.Can greatly satisfy the demand of people, alleviate pressure, protect ecology wild natural resources to the delicious edible mushroom of this kind.
Technical scheme of the present invention is: artificial culture method of fuscous dictyostelium boletes carries out according to the following steps:
1) the bacterium rod is cultivated
Cultivate dun net handle bolete bacterium rod with following solid culture medium, the preparation of bacterium rod is formulated by following raw material and method:
A. raw material is formed:
Rubber wood timber wood sawdust or other weed tree sawdusts 10.0～20.0kg
Grain 1.0～10.0kg
Flour 1.0～5.0kg
M
gSO
4 10.0～20.0g
KH
2PO
4 10.0～20.0g
pH 4.0～6.0
B. compound method: with putting into container after the grain weighing, soak 10～12h, the filtering excessive moisture is stand-by then; Accurately the amount of weighing flour is evenly admixed in rubber wood timber wood sawdust or the weed tree sawdust material, adds the grain that soaks behind the drainage then, with MgSO
4, KH
2PO
4With being sprayed into the wood sawdust stockpile after an amount of water-soluble the separating, the consumption of water is as the criterion with stockpile water content 60.0%, evenly mixes various raw materials thoroughly mixing at last, and medium PH4.0～6.0 promptly get solid culture medium; The solid culture medium for preparing is sub-packed in the polypropylene strain bag, sterilized 60 minutes for 120 ℃, standby after cooling;
C. inoculated and cultured: cultured phlebopus portentosus liquid bacterial is inoculated in the packed culture matrix among the step b in advance, places culturing room's half-light of 26～30 ℃ to cultivate 40～60 days culture bag after the inoculation, and mycelium covers with the bacterium bag, the bacterium rod;
2) artificial cultivation
A. bacterium rod earthing is cultivated fruiting
1. cover soil material preparation: work such as vegetable garden soil, orchard soil, paddy soils all can be made cover soil material; get the soil layer of top layer 20-30cm; the soil that takes out is tanned by the sun under burning sun to drying; smash then; decontamination; the screen cloth with aperture 1.5cm sieves; remove thick grogs; transfer soil moisture content 40-50%, admix 10% farmyard manure, are stacked in the threshing ground 40-60 days; cover double-plastic spontaneous fermentation and sterilization on the face; every interval 15-20 days turning 1 time, transfer sufficient soil moisture content 40-50% during turning at every turn, and continuous turning promptly gets cover soil material for 2-3 time;
2. earthing is cultivated fruiting
Open above-mentioned steps 1) the cultured bacterium of a. rod sack, the cover soil material that 1. step is prepared covers the surface of bacterium rod, and earthing bed thickness 3-8cm, the bacterium rod behind the earthing are put in plastic foam plate mushroom room, brick mixes in mushroom room and the green house and cultivates fruiting; Keep mushroom room, green house temperature 26-32 ℃, relative moisture 75-95%, illuminance 50-800LX, keep the overburden layer surface wettability, behind the earthing 10-15 days, promptly grow dun net handle bolete fruit body children flower bud on the face of bacterium rod overburden layer, a continued growth 5-6 days fruit body is reached maturity, the fruit body children flower bud of each bacterium rod growth is counted 1-20 and is not waited ripe number 1-2;
B. the bacterium rod takes off a bag earthing cultivation fruiting
1. cultivate ground: field, garden Geothermal Belt plant tree in the ranks or green house;
2. earthing method: in the field, in the ranks the reaching in the green house of garden Geothermal Belt plant tree, it is dark to dig 20-30cm, and the shallow ridges that length and width is fixed with demand waters permeablely in the ditch, treat that soil fully absorbs water, and is standby during soil moisture 60-80%; With above-mentioned steps 1) the cultured bacterium rod of c. is thrown in the shallow ridges after sloughing plastic film bag, bacterium rod interbody spacer 2-3cm, soil is inserted in the centre, earthing on the face of bacterium rod then, earthing bed thickness 3-8cm, the soil that 1. cover soil material prepares with the fine earth or the above-mentioned steps on field, garden ground all can, the cultivating bed top that earthing is cultivated fruiting is barricaded as little shed rain cover with plastic foil, bamboo chip and shades;
3. cultivate fruiting: keep overburden layer humidity, loose, temperature of shed 26-32 ℃, relative moisture 70-95%, illuminance 100-800LX cultivates fruiting, promptly grow fruit body and reaching maturity of the native face of 20 days cultivating beds behind the earthing;
C. inoculate tropical economic crops root system and tropical flower plant root system and cultivate fruiting
1. inoculation method: rainy season or other seasons the soil on field, ground, garden is watered permeable after, excavate the soil of pineapple honey tree, grapefruit tropical crop root, expose part master, the lateral root of root system; Excavate the soil of South America wedelia chinensis, the tropical flower plant root of peanut grass, expose part master, the lateral root of root system; Then will be in advance cultured solid spawn break into lumps off with the fingers and thumb, insert main root and lateral root portion, every strain inserts bacterial classification 50-500.0g, bacterial classification closely contacts with the stem foot of plant and master, lateral root portion as far as possible during inoculation;
2. cultivate fruiting: keep the root soil humidity of inoculation tree, the dun net handle bolete fruit body of promptly growing in inoculation back inoculation in the 30-60 days plant root surrounding soil and invention are ripe.
Artificial culture method of fuscous dictyostelium boletes of the present invention is the initiative of mycorhiza edible mushroom artificial cultivation method, and its characteristics and advantage are as follows:
1, cultivates into the bacterium rod with the pure culture mycelium, cultivate fruiting to the direct earthing of bacterium rod or after taking off bag earthing then, break away from the host and set the artificial cultivation of having realized real meaning.
2, bacterium rod medium composition is simple, and cultural method is easy, and incubation time is short: only used simple carbon nitrogen source (grain, flour) in the culture medium prescription, general mineral salt (MgSO
4, KH
2PO
4) and the wood sawdust of rubber wood timber or other weedtrees can carry out the bacterium rod and cultivate; Can carry out the bacterium rod with bacterium bag, general culturing room's (can heat) cultivates; Cultivating needs 60 days mycelia promptly to cover with the bacterium bag.
3, the fruit body cultural method is easy, and the neat cultivating rate height of fruiting: a, mixes at plastic foam plate mushroom and brick and can carry out bacterium rod earthing in mushroom room, the green house and cultivate fruiting; B, in field, garden ground and green house, can carry out the bacterium rod and take off bag earthing and cultivate a fruiting; C, inoculate tropical economic crops and the flower plant root system can be cultivated fruiting with solid spawn.
4, cultivate fruiting time weak point: bacterium rod earthing is cultivated fruiting only needs 10-15 days; The bacterium rod takes off bag earthing and cultivated fruiting 20 days; Inoculate tropical economic crops and flower plant root system with solid spawn and cultivated fruiting 30-60 days.
The present invention directly cultivates fruiting with being put in mushroom room or the green house behind the bacterium rod earthing, and the bacterium rod is taken off bag back earthing in field, ground, garden, green house cultivate the method for fruiting and be applicable to other kinds of bolete.The present invention is seeded in dun net handle beef liver solid spawn the method for cultivating fruiting on the root system of economic crops such as pineapple honey tree, grapefruit of field cultivation and is applicable to that the root system that is inoculated in other any tropical economic crops and flower plant cultivates fruiting.
Embodiment
Embodiment 1:
1. the bacterium rod is cultivated
Cultivate dun net handle bolete bacterium rod with following solid culture medium, the preparation of bacterium rod is formulated by following raw material and method.
1.1 raw material is formed:
Rubber wood timber wood sawdust or other weed tree sawdusts 10.0kg
Grain 5.0kg
Flour 1.0kg
M
gSO
4 10.0g
KH
2PO
4 10.0g
pH 4.0
1.2 compound method: container is put in the grain weighing, soaked 10～12h, the filtering excessive moisture is stand-by then.Accurately the amount of weighing flour is evenly admixed in rubber wood timber wood sawdust or the weed tree sawdust material, adds the grain that soaks behind the drainage then, with MgSO
4, KH
2PO
4With being sprayed into the wood sawdust stockpile after an amount of water-soluble the separating, the consumption of water is as the criterion with stockpile water content 60.0%, evenly mixes various raw materials thoroughly mixing at last, and medium nature PH4.0～6.0 get solid culture medium.The solid culture medium for preparing is sub-packed in the polypropylene strain bag of 17 * 30cm, every bag of 700ml sterilized 60 minutes for 120 ℃, and is standby after cooling;
1.3 inoculated and cultured: will be in advance cultured phlebopus portentosus liquid bacterial be inoculated in the packed composts or fertilisers of cultivating matrix in the step " 1.2 ", place culturing room's half-light of 26～30 ℃ to cultivate 40～60 days culture bag after the inoculation, mycelium covers with the bacterium bag, gets the bacterium rod.
2. artificial cultivation
2.1 bacterium rod earthing is cultivated fruiting
2.1.1 cover soil material preparation: get topsoil 20cm-30cm, soil aggregate is good, the vegetable garden soil that is rich in humus is made cover soil material, the soil that takes out is tanned by the sun to drying under burning sun, smash then, decontamination sieves with the screen cloth of aperture 1.5cm, removes thick grogs, admix 20% farmyard manure, transfer soil moisture content 40%-60%, cover double-plastic on the face, be stacked at spontaneous fermentation and sterilization in the threshing ground then, time 40-60 days, carry out at interval 1 turning in 15 days, 2-3 time continuously, transfer sufficient soil moisture content 40%-60% cover soil material during turning at every turn.
2.1.2 bacterium rod earthing is cultivated fruiting
Open the cultured bacterium of above-mentioned steps " 1.3 " rod sack, the vegetable garden soil cover soil material of step " 2.2.1 " preparation is covered in the surface of bacterium rod, earthing bed thickness 4-6cm, the bacterium rod behind the earthing is put in the plastic foam plate mushroom room and cultivates fruiting.Keep room temperature 26-30 ℃ in mushroom, relative moisture 85%-95%, illuminance 100-300LX keeps the overburden layer surface wettability.Behind the earthing 10-12 days, the dun net handle bolete fruit body children flower bud that promptly grows on the bacterium rod earthing aspect, a continued growth 5-6 days fruit body is reached maturity.The fruit body children flower bud of each bacterium bag growth is counted 1-20 and is not waited ripe number 1-2.
2.1.3 the evaluation of artificial culture fruit body kind
2.1.3.1 identification of morphology: the sporophore shape of artificial culture, its feature is as follows: the cap yellowish-brown, pitchy, 5-14cm, the lid center projections, ripe back is open and flat, flawless, the lid edge upwarps more.The thick 2-6cm of lid meat, soft, spongioplasm, faint yellow, seldom present light blue after the wound.Bacterium hole single-orifice type, yellow.The long 1.0-2.0cm of tube, the aperture is near round to multiangular, yellow or pitchy.Stem is sturdy, and base portion often expands and is the mortar shape, and 2-3 * 6-9cm is several homochromy with cap.The long pears type of basidiospore, long ellipse, 7.5-13.0um * 5.0-7.45um, olive-green.In sum, the fruit body of artificial culture is consistent with wild fruit body kenel feature, can be accredited as same kind.The fruit body voucher specimen of artificial culture is placed on Kunming Inst. of Botany, Chinese Academy of Sciences fungus specimen chamber, is numbered HKAS 49706.
2.1.3.2 Molecular Identification: the fruit body of Molecular Identification artificial culture, the autoploidy of its ITS sequence and wild dun net handle bolete fruit body can be accredited as same kind more than 99%.
2.2 taking off bag earthing, the bacterium rod cultivates fruiting
2.2.1 cultivate ground: field pineapple honey tree in the ranks
2.2.2 earthing method: field pineapple honey tree in the ranks, it is dark to dig 20-30cm, and the shallow ridges of long 5m, wide 60cm waters permeablely in the ditch, treat that soil fully absorbs water, and soil moisture is that 60-80% is standby.Be thrown in the shallow ridges after the cultured bacterium rod of above-mentioned steps " 1.3 " sloughed plastic film bag, bacterium rod interbody spacer 2-3cm, soil is inserted in the centre, earthing on the face of bacterium rod then, earthing bed thickness 4-6cm.Cover soil material pineapple honey tree fine earth in the ranks.The cultivating bed top of earthing cultivation fruiting is barricaded as little shed rain cover with plastic foil, bamboo chip and shades.
2.2.3 cultivation fruiting: keep overburden layer humidity, loose, temperature of shed 26-32 ℃, relative moisture 80-90%, illuminance 100-300LX cultivates fruiting.Look unfamiliar eldest son's entity and reaching maturity of 20d cultivating bed soil behind the earthing.
2.3 fruiting is cultivated by the jack fruit tree root system of inoculation field cultivation
2.3.1 inoculation method: after rainy season, field soil was drenched, excavate the root soil of pineapple honey tree, expose part master, the lateral root of root system; Cultured solid spawn breaks into lumps off with the fingers and thumb in advance then, inserts main root and lateral root portion.Every strain inserts bacterial classification 200.0g, and bacterial classification closely contacts with the stem foot of pineapple honey tree and master, lateral root as far as possible during inoculation.
2.3.2 cultivation fruiting: keep the root soil humidity of inoculation tree, inoculate back 30 days and promptly grow in the jack fruit usage tree root surrounding soil dun net handle bolete fruit body and reach maturity.
Embodiment 2:
1. the bacterium rod is cultivated
Cultivate dun net handle bolete bacterium rod with following solid culture medium, the preparation of bacterium rod is formulated by following raw material and method.
1.1 raw material is formed:
Rubber wood timber wood sawdust or other weed tree sawdusts 15.0kg
Grain 8.0kg
Flour 3.0kg
M
gSO
4 15.0g
KH
2PO
4 15.0g
pH 5.0
Following compound method and inoculated and cultured are with embodiment 1.
2. artificial cultivation
2.1 bacterium rod earthing is cultivated fruiting
2.1.1 the cover soil material preparation: make cover soil material with orchard soil, the preparation method is with embodiment 1.
2.1.2 bacterium rod earthing is cultivated fruiting
With cultured bacterium bastinade opened mouth, the cover soil material for preparing directly is covered in the surface of bacterium rod, earthing bed thickness 4-6cm, the bacterium rod behind the earthing are put in brick and mix in the mushroom room and cultivate fruiting.All the other management methods are with embodiment 1.
2.2 taking off bag earthing, the bacterium rod cultivates fruiting
2.2.1 cultivate ground: the field grapefruit in the ranks.
2.2.2 earthing method: in the ranks the field grapefruit, it is dark to dig 20-30cm, the shallow ridges of long 5m, wide 60cm, water permeable in the ditch, be thrown in the shallow ridges after treating after soil fully absorbs water cultured bacterium rod to be sloughed plastic film bag, bacterium rod interbody spacer 2-3cm, soil is inserted in the centre, earthing on the face of bacterium rod then, earthing bed thickness 6cm.Cover soil material is with grapefruit fine earth in the ranks.The cultivating bed top is barricaded as little shed rain cover with plastic foil, bamboo chip and shades.
2.2.3 cultivation fruiting: with embodiment 1.
2.3 inoculation field flame-tree root system is cultivated fruiting
Every strain inserts bacterial classification 100.0g, and all the other inoculation methods and cultivation fruiting are with embodiment 1.
Embodiment 3
1. the bacterium rod is cultivated
Cultivate dun net handle bolete bacterium rod with following solid culture medium, the preparation of bacterium rod is formulated by following raw material and method.
1.1 raw material is formed:
Rubber wood timber wood sawdust or other weed tree sawdusts 20.0kg
Grain 10.0kg
Flour 5.0kg
M
gSO
4 20.0g
KH
2PO
4 20.0g
pH 5.0
Following compound method and inoculated and cultured are with embodiment 1.
2. artificial cultivation
2.1 bacterium rod earthing is cultivated fruiting
2.1.1 the cover soil material preparation: make cover soil material with paddy soils, the preparation method is with embodiment 1.
2.1.2 bacterium rod earthing is cultivated fruiting
With cultured bacterium bastinade opened mouth, the cover soil material for preparing directly is covered in the surface of bacterium rod, earthing bed thickness 6cm, the bacterium rod behind the earthing is put in and cultivates fruiting in the green house.All the other management methods are with embodiment 1.
2.2 taking off bag earthing, the bacterium rod cultivates fruiting
2.2.1 cultivate ground: green house.
2.2.2 earthing method: on the ground of green house, it is dark to dig 10cm-20cm, length is not limit, the shallow ridges of wide 60cm-80cm, water permeable in the ditch, be thrown in the shallow ridges after treating after soil fully absorbs water cultured bacterium rod to be sloughed plastic film bag, bacterium rod interbody spacer 2cm-3cm, soil is inserted in the centre, earthing around on the face of bacterium rod, reaching then, earthing bed thickness 4cm-6cm.The previously prepared cover soil material of cover soil material.
2.2.3 cultivate the fruiting method: with embodiment one.
2.3 the wounded in the battle grass of vaccinal fever South America wedelia chinensis root system is cultivated fruiting
Every strain inserts bacterial classification 100.0g, and all the other inoculation methods and cultivation fruiting are with embodiment 1.
Claims (1)
1. artificial culture method of fuscous dictyostelium boletes is characterized in that reaching according to the following steps method and carries out:
1) the bacterium rod is cultivated: cultivate dun net handle bolete bacterium rod with following solid culture medium, the preparation of bacterium rod is formulated by following raw material and method:
A. raw material is formed:
Rubber wood timber wood sawdust or other weed tree sawdusts 10.0～20.0kg
Grain 1.0～10.0kg
Flour 1.0～5.0kg
MgSO
4 10.0～20.0g
KH
2PO
4 10.0～20.0g
pH 4.0～6.0
B. compound method: with putting into container after the grain weighing, soak 10～12h, the filtering excessive moisture is stand-by then; Accurately the amount of weighing flour is evenly admixed in rubber wood timber wood sawdust or the weed tree sawdust material, adds the grain that soaks behind the drainage then, with MgSO
4, KH
2PO
4With being sprayed into wood sawdust or weed tree sawdust stockpile after an amount of water-soluble the separating, the consumption of water is as the criterion with stockpile water content 60.0%, evenly mixes various raw materials thoroughly mixing at last, and medium PH 4.0～6.0 promptly gets solid culture medium; The solid culture medium for preparing is sub-packed in the polypropylene strain bag, sterilized 60 minutes for 120 ℃, standby after cooling;
C. inoculated and cultured: cultured phlebopus portentosus liquid bacterial is inoculated in the packed culture matrix among the step b in advance, places culturing room's half-light of 26～30 ℃ to cultivate 40 days～60 days culture bag after the inoculation, and mycelium covers with the bacterium bag, the bacterium rod;
2) artificial cultivation
A. bacterium rod earthing is cultivated fruiting
1. cover soil material preparation: vegetable garden soil, orchard soil or paddy soils are made cover soil material; get the soil layer of top layer 20-30cm; the soil that takes out is tanned by the sun to drying under burning sun; smash then; decontamination; the screen cloth with aperture 1.5cm sieves; remove thick grogs; transfer soil moisture content 40-50%, admix 10% farmyard manure, are stacked in the threshing ground 40-60 days; cover double-plastic spontaneous fermentation and sterilization on the face; every interval 15-20 days turning 1 time, transfer sufficient soil moisture content 40-50% during each turning, and continuous turning promptly gets cover soil material 2-3 time;
2. earthing is cultivated fruiting
Open above-mentioned steps 1) the cultured bacterium of c. rod sack, the cover soil material that 1. step is prepared covers the surface of bacterium rod, and earthing bed thickness 3-8cm, the bacterium rod behind the earthing are put in plastic foam plate mushroom room, brick mixes in mushroom room or the green house and cultivates fruiting; Keep mushroom room, green house temperature 26-32 ℃, relative moisture 75-95%, illuminance 50-800LX, keep the overburden layer surface wettability, behind the earthing 10-15 days, promptly grow dun net handle bolete fruit body children flower bud on the face of bacterium rod overburden layer, a continued growth 5-6 days fruit body is reached maturity;
B. the bacterium rod takes off a bag earthing cultivation fruiting
1. cultivate ground: field, garden Geothermal Belt plant tree in the ranks or green house;
2. earthing method: in the field, in the ranks the reaching in the green house of garden Geothermal Belt plant tree, it is dark to dig 20-30cm, and the shallow ridges that length and width is fixed with demand waters permeablely in the ditch, treat that soil fully absorbs water, and is standby during soil moisture 60-80%; With above-mentioned steps 1) the cultured bacterium rod of c. is thrown in the shallow ridges after sloughing plastic film bag, bacterium rod interbody spacer 2-3cm, soil is inserted in the centre, earthing on the face of bacterium rod then, earthing bed thickness 3-8cm, cover soil material is with the fine earth or the above-mentioned steps 2 on field, garden ground) cover soil material that 1. prepares of a., the cultivating bed top that earthing is cultivated fruiting is with the shed rain cover and shade;
3. cultivate fruiting: keep overburden layer humidity, loose, temperature of shed 26-32 ℃, relative moisture 70-95%, illuminance 100-800LX cultivates fruiting, promptly grow fruit body and reaching maturity of the native face of 20 days cultivating beds behind the earthing;
C. inoculate tropical economic crops root system and tropical flower plant root system and cultivate fruiting
1. inoculation method: rainy season or other seasons the soil on field, ground, garden is watered permeable after, excavate the soil of pineapple honey tree, grapefruit tropical crop root, expose part master, the lateral root of root system; Excavate the soil of South America wedelia chinensis, the tropical flower plant root of peanut grass, expose part master, the lateral root of root system; Then will be in advance cultured solid spawn break into lumps off with the fingers and thumb, insert main root and lateral root portion, every strain inserts bacterial classification 50-500.0g, bacterial classification closely contacts with the stem foot of plant and master, lateral root portion as far as possible during inoculation;
2. cultivate fruiting: keep the root soil humidity of inoculation tree, the dun net handle bolete fruit body of promptly growing in inoculation back inoculation in the 30-60 days plant root surrounding soil is also reached maturity;
A, b, c three-type-person worker cultivate optional wherein a kind of of fruiting mode.
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| CN102476973A (en) * | 2011-11-10 | 2012-05-30 | 云南省热带作物科学研究所 | Phlebopus portentosus artificial cultivation casing soil material |
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| CN102870597B (en) * | 2012-09-27 | 2013-08-28 | 云南省热带作物科学研究所 | Phlebopus portentosus strain |
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| CN103609338B (en) * | 2013-12-19 | 2015-09-30 | 云南大学 | The abductive approach of brick red suede lid bolete test tube fruit body primordium |
| CN104126416B (en) * | 2014-08-21 | 2016-03-30 | 石泉 | A kind of Boletus aereus merit domesticating method |
| CN104892095A (en) * | 2015-05-08 | 2015-09-09 | 云南省热带作物科学研究所 | Mould-inhibition and yield-increasing panacea dedicated to soil covering process for Phlebopus portentosus |
| CN105794496A (en) * | 2016-03-22 | 2016-07-27 | 景洪宏臻农业科技有限公司 | Industrialized boletus aereus bottle-culture method |
| CN107318467A (en) * | 2017-08-23 | 2017-11-07 | 佛山推启农业研究院（普通合伙） | A kind of method of the blood red bolete of artificial cultivation |
| CN109097288A (en) * | 2018-08-02 | 2018-12-28 | 周茂 | A kind of fluid nutrient medium, the preparation method of Phlebopus portentosus batch production liquid spawn and the purposes of the fluid nutrient medium |
| CN109042063A (en) * | 2018-08-20 | 2018-12-21 | 周茂 | A kind of culture medium for cultivating, preparation method and a kind of Phlebopus portentosus batch production bacterium bag cultural method |
| CN110574638B (en) * | 2019-10-29 | 2021-10-19 | 遵义市林业科学研究所 | A device that is used for field inoculation of delicious boletus |
| CN112568061B (en) * | 2020-12-25 | 2024-03-12 | 云南省热带作物科学研究所 | Preparation method and application of making Phlebopus portentosus cultivar from dry cassava slices |
| CN113348963B (en) * | 2021-06-07 | 2022-06-17 | 云南省热带作物科学研究所 | Artificial cultivation method of Boletus sinensis (Boletus) Boletus (pers.) Ricken |
| CN117025415B (en) * | 2023-08-17 | 2024-06-18 | 景洪宏臻农业科技有限公司 | Phlebopus portentosus strain V239.04 and application thereof |
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