CN103766137B - A kind of Boletus aereus cultural method - Google Patents
A kind of Boletus aereus cultural method Download PDFInfo
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- CN103766137B CN103766137B CN201310546045.4A CN201310546045A CN103766137B CN 103766137 B CN103766137 B CN 103766137B CN 201310546045 A CN201310546045 A CN 201310546045A CN 103766137 B CN103766137 B CN 103766137B
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Abstract
The present invention relates to a kind of cultivation Boletus aereus (<i>Phlebopus? portentosus</i>) method, comprise culture matrix preparation, inoculation bacteria, earthing, overburden layer cultural hypha, mushroom producing culture step. The invention still further relates to the purposes in a kind of fungi culture matrix and Boletus aereus cultivation thereof.
Description
Technical field
The present invention relates to a kind of Boletus aereus cultural method, belong to edible fungus cultivation technique field.
Background technology
Boletus aereus (Phlebopusportentosus), claim again Phlebopus portentosus, be a kind of facultative mycorhiza edible mushroom in the torrid zone of preciousness, be distributed in Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia, the ground such as New Zealand and Yunnan Province of China, Guangxi and Hainan. Boletus aereus quality is fresh and tender, delicious flavour, nutritive value abundant, high protein, low fat, be rich in the necessary mineral elements of human body such as 17 kinds of essential amino acids and abundant phosphorus, potassium, calcium, magnesium, iron, zinc, be good healthy food and there is good medical value, liked by consumer. Most boletes are mycorhiza edible mushroom. The process of the semi-artificial simulation cultivation of mycorhiza edible mushroom is quite long, needs 4～5 years, even within 6～7 years, just can complete. Only there is at present the bolete of a few species can carry out semi-artificial simulation cultivation and manual simulation cultivates in laboratory research state. As micro-suede bolete (B.subtomentosus), can carry out artificial cultivation (Harley&Smith, 1983) with the cultural method of saprophytic bacteria. Suillus lutens (Suillusluteus), with synthetic Va Mycorrhiza Seedling dense planting in through sterilization exclosure realize artificial cultivation, Va Mycorrhiza Seedling plant to suillus lutens sporophore growth need 12 months.
Although reported the mode of success of the test cultivation saprophytic bacteria and inoculated the host tree such as flame tree, coffee, shaddock, mango host tree and carry out a small amount of artificial cultivation Boletus aereus, but because of cultural method immature, promote the large product quantity of difficulty extremely limited, the Boletus aereus of selling in the market still picks up from wild self-sow. Because limiting output of climate condition in season is less, add the protection of not focusing on wild self-sow resource, artificial excessively harvesting causes quantity fewer and feweri. Therefore, there is the active demand of the Boletus aereus cultural method to simple and effective in edible fungus cultivation field.
Summary of the invention
The invention provides a kind of Boletus aereus cultural method and cultivation culture matrix. This cultural method is easy, and incubation time is short, and fruiting is neat, maturing rate is high, receives bolete 1-2 for every bottle, produces fresh bolete 100g-120g, and biologicak efficiency 25.0% has reached the level of Volvaria volvacea cultivation and has been easy to and promoted. The method is that bolete is tame pioneering, realizes Boletus aereus factory culture for next step, realizes fast industrialization and has established solid foundation.
One aspect of the present invention relate to a kind of cultivation Boletus aereus (Phlebopusportentosus) method, comprising culture matrix preparation, inoculation bacteria, earthing, overburden layer cultural hypha, mushroom producing culture step, wherein said culture matrix comprises:
Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏
Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏
Red soil 10.0～20.0 ㎏
Wheat 5.0～10.0 ㎏
Rice bran 1.0～5.0 ㎏
Calcium carbonate 1.0～2.0 ㎏
MgSO410.0～20.0g
KH2PO410.0～20.0g
ZnSO410.0～20.0g
Yeast diffusion juice 10.0～20.0g
pH4.0～6.0。
In a detailed description of the invention, in described culture matrix, flame tree, grapefruit, mango or other weedtree material need be ground into wood chip, chip dimensions 0.2cm～0.4cm × 1.0cm～1.5cm, prewet fermentation reactor system 30 days-60 days of wood chip; Prewet fermentation reactor system 60 days-90 days of wood chip, turning in every 30 days is once within the fermentation reactor system phase for wood chip and wood chip.
In a detailed description of the invention, culture vessel is 1400ml～1650ml plastic bottle.
In a detailed description of the invention, culture vessel is 1400ml plastic bottle as shown in Figure 1.
In a detailed description of the invention, culture vessel is 1650ml plastic bottle as shown in Figure 2.
In a detailed description of the invention, in the time that culture matrix is filled to culture vessel, vessel port prewet stay the space of 4 ㎝-5 ㎝ height to cover with matrix for mycelia after earthing cultivate fruiting.
In a detailed description of the invention, inoculation bacteria is selected from and connects liquid spawn and connect solid spawn.
In a detailed description of the invention, bacteria condition is: 27 DEG C～31 DEG C of temperature, CO２Concentration 800PPm-1500PPm, humidity 60%-75%, half-light, cultivates 30 days-50 days mycelia and covers with matrix.
In a detailed description of the invention, cover soil material is top layer 60 ㎝ red soils, top layer 30 ㎝ rubber garden mould or orchard soils, and each bacterium bottle earthing 250g-300g, overburden layer thickness and bottleneck are flat. In a further detailed description of the invention also, cover soil material further comprise add 30%(volume ratio) wet turfy soil, soil moisture content 60%--70%.
In a detailed description of the invention, overburden layer cultural hypha condition is: 27 DEG C～31 DEG C of temperature, CO２Concentration 1000PPm-2000PPm, humidity 85%-90%, half-light, in incubation, artificial spraying keeps overburden layer surface wettability, cultivates 8 days-10 days mycelia and covers with overburden layer.
In a detailed description of the invention, mushroom producing culture condition is: 26 DEG C～30 DEG C of temperature, CO２Concentration 500PPm-1000PPm, humidity 90%-95%, with LED light source, illuminance 50LX-250LX, 8 hours every days of light application time, cultivates and within the 2nd day, starts fruiting, continue to cultivate 5 days-7 days, each blake bottle can a ripe 1-2 mushroom, in the time that mushroom lid does not launch completely, gathers in time.
In a detailed description of the invention, whole incubation in a culture vessel, complete and without again transplant.
In a detailed description of the invention, the cultivation of 1400ml plastic bottle, every bottle is produced the heavy 80g-120g of single mushroom, the heavy 100g of average single mushroom; The cultivation of 1650ml plastic bottle, every bottle is produced the heavy 100g-150g of single mushroom, the heavy 120g(of average single mushroom is shown in Fig. 3).
The present invention relates to a kind of Boletus aereus bottle cultivating method on the other hand, comprises the steps:
(1) culture medium preparation
With the solid culture based formulas cultivation Boletus aereus of improvement, solid culture based formulas and compound method are as follows:
A. solid culture based formulas:
Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏
Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏
Red soil 10.0～20.0 ㎏
Wheat 5.0～10.0 ㎏
Rice bran 1.0～5.0 ㎏
Calcium carbonate 1.0～2.0 ㎏
MgSO410.0～20.0g
KH2PO410.0～20.0g
ZnSO410.0～20.0g
Yeast diffusion juice 10.0～20.0g
pH4.0～6.0。
B. raw material is prepared:
The log of b1 flame tree, grapefruit, mango or other weedtrees or branch are ground into wood chip and the saw dust of 0.2cm～0.4cm × 1.0cm～1.5cm specification, then prewet, build heap, keep wood chip and saw dust water content 60% to carry out fermentation reactor system, wood chip fermentation reactor system 30 days-60 days, wood chip fermentation reactor system 90 days-120 days, in the fermentation reactor system phase, turning in every 30 days once, 2-3 time continuously, allows wood chip and saw dust fully soften, to become thoroughly decomposed.
It is powder that b2 red soil dries rear pulverizing.
C. compound method:
Accurately weigh flame tree, grapefruit, mango or other weedtree wood chips that step b1 fully ferments and saw dust each 50%, mix, then evenly admix the red soil powder of step b2, stack and within 10-12 hour, allow the even moisture absorption of soil be beneficial to thorough sterilizing. Wheat berry is soaked 10～12h, and filtering excessive moisture is stand-by. Wheat berry after rice bran, calcium carbonate and immersion is admixed in above-mentioned stockpile, MgSO4、KH2PO4、ZnSO4, Yeast diffusion juice water dissolve after be evenly sprayed in stockpile, finally various raw materials are evenly mixed, water content of substrate 60.0%, PH4.0～6.0, obtain solid culture matrix;
(2) bottling, sterilizing
Make blake bottle (seeing Fig. 1, Fig. 2) with the large capacity plastic bottle of 1400ml～1650ml, the solid medium preparing is sub-packed in plastic bottle, 1400ml plastic bottle wet feed weighs 0.85 ㎏, and 1650ml plastic bottle wet feed weighs 1.0 ㎏. Punch to bottom in centre position in matrix after bottling, bore dia 2 ㎝, and shoulder is (after covering with matrix for mycelia, earthing is cultivated fruiting) to the high space of reserved 4 ㎝ of bottleneck, and 121 DEG C of sterilizings 70 minutes are for subsequent use after cooling.
(3) inoculation, bacteria
Will be in advance cultured Boletus aereus liquid spawn or solid spawn be inoculated in the sterilized training base of step (2), every bottle graft liquid spawn 20ml or meet solid spawn 50g. After inoculation, cultivation is put to bottle and be placed in 27 DEG C～31 DEG C of temperature, CO２Concentration 800PPm-1500PPm, the culturing room of humidity 60%-75%, cultivates 30 days-50 days mycelia and covers with matrix and complete the bacteria stage under half-light condition.
(4) earthing
Mycelia must be in the surface of bacterium bottle ability fruiting and fruit body development maturation after covering with matrix.
Cover soil material I: get top layer 60 ㎝ red soils, top layer 30 ㎝ rubber garden mould or orchard soils. Red soil is homogeneous, not containing sandstone, without sieving. Rubber garden mould or orchard soil need sieve, and remove sandstone. Then red soil, rubber garden mould or orchard soil are watered and prewetted, soil water content reaches 60.0%, stacks 10 days-30 angel's soil and evenly absorbs moisture content, loose.
Cover soil material II: cover soil material I is added to 30%(volume ratio) wet turfy soil, mix water content 60%.
Above-mentioned steps (3) completes the bacterium bottle of cultural hypha, open bottle cap, stack 10 days-30 days prewetting, loose red soil rubber garden mould or other orchard soils are covered in stromal surface, each bacterium bottle earthing 250g-300g, earthing is to flat with bottleneck, thickness 4 ㎝-5 ㎝, after earthing, do not cover bottle cap, appropriateness shake bottle, can make soil fully contact with matrix mycelia like this, can keep again the suitable elasticity of overburden layer to ensure good aeration status, be beneficial to the growth of bolete mycelium germination and enter fast overburden layer, covering with overburden layer, fruiting.
(5) overburden layer cultural hypha
Bacterium bottle after step (4) earthing is at 27 DEG C～31 DEG C of temperature, CO２Concentration 1000PPm-2000PPm, in the cultivation room of humidity 85%-90%, half-light, cultivate the bolete mycelia overburden layer of growing into, except keeping cultivating the humidity 85%-90% of space, room, the overburden layer charge level that also needs manually to spray keeps the moistening mycelial growth that is beneficial to, after 8 days-10 days, mycelia grows to overburden layer surface, completes overburden layer cultural hypha.
(6) mushroom producing culture
Complete the bacterium bottle of overburden layer cultural hypha through above-mentioned steps (5), since the 11st day, cultivation room temperature is down to 26 DEG C～30 DEG C, CO２Concentration is adjusted to 500PPm-1000PPm, humidity 90%-95%, 8 hours every days of light application time, the morning 9:00 to afternoon 5:00 carry out illumination, under this condition, cultivate the 2nd day, in bacterium bottle earthing aspect, start fruiting, each bacterium bottle can fruiting 1-50 a young flower bud, continue to cultivate 5-7 days, each bacterium bottle can ripe 1-2 bolete fructification, in the time that bolete cap launches not yet completely, gathers in time. The cultivation of 1400ml plastic bottle, every bottle is produced the heavy 80g-120g of single mushroom, the heavy 100g of average single mushroom; The cultivation of 1650ml plastic bottle, every bottle is produced the heavy 100g-150g of single mushroom, the heavy 120g(of average single mushroom is shown in Fig. 3).
The present invention relates to a kind of fungi culture matrix on the other hand, and it comprises:
Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏
Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏
Red soil 10.0～20.0 ㎏
Wheat 5.0～10.0 ㎏
Rice bran 1.0～5.0 ㎏
Calcium carbonate 1.0～2.0 ㎏
MgSO410.0～20.0g
KH2PO410.0～20.0g
ZnSO410.0～20.0g
Yeast diffusion juice 10.0～20.0g
pH4.0～6.0。
In a detailed description of the invention, in described culture matrix, flame tree, grapefruit, mango or other weedtree material need be ground into wood chip, chip dimensions 0.2cm～0.4cm × 1.0cm～1.5cm, prewet fermentation reactor system 30 days-60 days of wood chip; Prewet fermentation reactor system 60 days-90 days of wood chip, turning in every 30 days is once within the fermentation reactor system phase for wood chip and wood chip.
The present invention relates to the purposes of a kind of culture matrix in Boletus aereus is cultivated on the other hand, and described culture matrix comprises:
Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0kg
Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0kg
Red soil 10.0～20.0kg
Wheat 5.0～10.0kg
Rice bran 1.0～5.0kg
Calcium carbonate 1.0～2.0kg
MgSO410.0～20.0g
KH2PO410.0～20.0g
ZnSO410.0～20.0g
Yeast diffusion juice 10.0～20.0g
pH4.0～6.0。
The composition of the culture matrix the present invention relates to includes but not limited to flame tree, grapefruit, mango or other weedtree wood chips, flame tree, grapefruit, mango or other weedtree wood chips, red soil, wheat, rice bran, calcium carbonate, MgSO4，KH2PO4，ZnSO4And Yeast diffusion juice.
As used herein, number range comprises any number between higher limit, lower limit and higher limit and lower limit. For example, " flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0kg " refers to that the content of flame tree, grapefruit, mango or other weedtree wood chips can be any number option between 5.0kg, 10.0kg and 5.0kg and 10.0kg, such as 5.01kg, 6.0kg, 7.5kg, 9.0kg, 9.99kg etc.
Bolete saprophytic indifferent, therefore needs the growth of the nutrition supply mushroom that certain base quality assurance is enough. The invention provides the method that uses the large capacity blake bottle cultivation of defined medium matter utilization Boletus aereus, not only ensure the growth of the nutrition supply mushroom that enough matrix amounts contain, guarantee that fructification weighs more than 100g, and the thick wood chip having solved because containing in cultivation matrix is easy to polybag to expose, the problem that causes culture period bacterium bag to pollute, realize full-automatic factory culture for next step, realize industrialization and established solid foundation. Bottle is planted Boletus aereus industrialization and is cultivated successfully, can greatly meet the demand of people to this kind of delicious edible mushroom, alleviates the pressure to wild natural resources, and protection is ecological, has higher using value, economic implications and social effect.
Brief description of the drawings
Fig. 1 shows the schematic diagram of an example of 1400ml plastic bottle.
Fig. 2 shows the schematic diagram of an example of 1650ml plastic bottle.
Fig. 3 is presented at the fruiting situation photo of cultivating in 1650ml plastic bottle.
Detailed description of the invention
In following examples, further describe the present invention, described embodiment shows and implements concise and to the point method of the present invention, but does not limit its likely modification of institute.
Embodiment 1
(1) culture medium preparation
With the solid culture based formulas cultivation Boletus aereus of improvement, solid culture based formulas and compound method are as follows:
A. solid culture based formulas:
Flame tree, grapefruit, mango or other weedtree wood chips 5.0 ㎏
Flame tree, grapefruit, mango or other weedtree wood chips 5.0 ㎏
Red soil 10.0 ㎏
Wheat berry 5.0 ㎏
Rice bran 1.0 ㎏
Calcium carbonate 1.0 ㎏
MgSO410.0g
KH2PO410.0g
ZnSO410.0g
Yeast diffusion juice 10.0g
pH4.0。
B. raw material is prepared:
The log of b1 flame tree, grapefruit, mango or other weedtrees or branch are ground into wood chip and the saw dust of 0.3cm × 1.0cm specification, then prewet, build heap, keep wood chip and saw dust water content 60% to carry out fermentation reactor system, wood chip fermentation reactor system 50 days, wood chip fermentation reactor system 90 days, in the fermentation reactor system phase, turning in every 30 days once, 2-3 time continuously, allows wood chip and saw dust fully soften, to become thoroughly decomposed.
It is powder that b2 red soil dries rear pulverizing.
C. compound method:
Accurately weigh flame tree, grapefruit, mango or other weedtree wood chips that step b1 fully ferments and saw dust each 50%, mix, then evenly admix the red soil powder of step b2, stack and within 10-12 hour, allow the even moisture absorption of soil be beneficial to thorough sterilizing. Wheat berry is soaked 10～12h, and filtering excessive moisture is stand-by. Wheat berry after rice bran, calcium carbonate and immersion is admixed in above-mentioned stockpile, MgSO4、KH2PO4、ZnSO4, Yeast diffusion juice water dissolve after be evenly sprayed in stockpile, finally various raw materials are evenly mixed, water content of substrate 60.0%, PH4.5, obtains solid culture matrix;
(2) bottling, sterilizing
Make blake bottle with the large capacity plastic bottle of 1650ml, the solid medium preparing is sub-packed in plastic bottle, every bottled wet feed weighs 1.0 ㎏, punch to bottom in centre position in matrix after bottling, bore dia 2 ㎝, shoulder is (after covering with matrix for mycelia, earthing is cultivated fruiting) to the high space of reserved 4 ㎝ of bottleneck, and 121 DEG C of sterilizings 70 minutes are for subsequent use after cooling.
(3) inoculation, bacteria
Will be in advance cultured Boletus aereus liquid spawn or solid spawn be inoculated in the sterilized training base of step (2), every bottle graft liquid spawn 20ml or meet solid spawn 50g. After inoculation, cultivation is put to bottle and be placed in 27 DEG C～29 DEG C of temperature, CO２Concentration 800PPm-1000PPm, the culturing room of humidity 60%-70%, cultivates 40 days mycelia and covers with matrix and complete the bacteria stage under half-light condition.
(4) earthing
Above-mentioned steps (3) completes the bacterium bottle of cultural hypha, open bottle cap, above-mentioned cover soil material I is prewetted and stacked 30 days, loose red soil or rubber garden mould or other orchard soils are covered in stromal surface, each bacterium bottle earthing 260g-300g, earthing is to flat with bottleneck, average thickness 4.5 ㎝, after earthing, do not cover bottle cap, appropriateness shake bottle, can make soil fully contact with matrix mycelia like this, and suitable elasticity has good aeration status to keep overburden layer again, be beneficial to the growth of bolete mycelium germination and enter fast overburden layer, covering with overburden layer, fruiting.
(5) overburden layer cultural hypha
Bacterium bottle after step (4) earthing is at 27 DEG C～29 DEG C of temperature, CO２Concentration 1000PPm-1500PPm, in the cultivation room of humidity 85%-90%, half-light, cultivate the bolete mycelia overburden layer of growing into, except keeping cultivating the humidity 85%-90% of space, room, the overburden layer charge level that also needs manually to spray keeps the moistening mycelial growth that is beneficial to, within 10 days, mycelia grows to overburden layer surface, completes overburden layer cultural hypha.
(6) mushroom producing culture
Complete the bacterium bottle of overburden layer cultural hypha through above-mentioned steps (5), since the 11st day, cultivation room temperature is down to 26 DEG C～28 DEG C, CO２Concentration is adjusted to 500PPm-700PPm, humidity 90%-95%, 8 hours every days of light application time, the morning 9:00 to afternoon 5:00 carry out illumination, under this condition, cultivate the 2nd day, in bacterium bottle earthing aspect, start fruiting, each bacterium bottle can fruiting 1-50 a young flower bud, continue to cultivate 5-7 days, each bacterium bottle can ripe 1-2 bolete fructification, in the time that bolete cap launches not yet completely, gather in time, every bottle is produced the heavy 100g-150g of single mushroom, and average every bottle is produced fresh mushroom 120g.
Embodiment 2-3
Embodiment 2-3 culture medium preparation, bottling sterilizing, inoculation bacteria, earthing and overburden layer cultural hypha, mushroom producing culture are identical with embodiment 1, and difference is in table 1.
Table 1 embodiment 1-3 different substrates formula and cultivation output
Embodiment 4
Embodiment 4 culture mediums preparations, bottling sterilizing, inoculation bacteria, earthing and overburden layer cultural hypha, mushroom producing culture are identical with embodiment 2-3, difference is that blake bottle is 1400ml plastic bottle, every bottled culture medium weight in wet base 0.85 ㎏, every bottle is produced the heavy 80g-120g of single mushroom, and average every bottle is produced fresh mushroom 100g.
Embodiment 5
Embodiment 5 culture mediums preparations, bottling sterilizing, inoculation bacteria, earthing and overburden layer cultural hypha, mushroom producing culture are identical with embodiment 1, and difference is that covering bacterium bottle by cover soil material II cultivates fruiting, average every bottle of fresh mushroom 120g of product.
Embodiment 6-7
Embodiment 6-7 culture medium preparation, bottling sterilizing, inoculation bacteria, earthing and overburden layer cultural hypha, mushroom producing culture are identical with embodiment 1, and difference is in table 2. As can be seen from Table 2, in bacteria, overburden layer cultural hypha, mushroom producing culture process, suitable high temperature, low CO２It is heavy that concentration is conducive to increase fresh mushroom. As embodiment 7,29 DEG C-31 DEG C of bacteria phase temperature, CO２Concentration 800ppm-1000ppm. 29 DEG C-31 DEG C of overburden layer cultural hypha phase temperature, CO２Concentration 1000ppm-1500ppm. 28 DEG C-30 DEG C of mushroom producing culture phase temperature, CO２Concentration 500ppm-700ppm, 100g-150g, average fresh mushroom substance 125g, higher than embodiment 1(120g) and embodiment 6(10g); .
Table 2 embodiment 1, embodiment 6-7 bacteria and overburden layer cultural hypha temperature, CO２Concentration and mushroom are heavy
Finally, note also that, what more than enumerate is only several specific embodiments of the present invention. Objectively, the present invention is not merely only limited to above embodiment, can also have quite a few variation. Therefore, all changes that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, are all considered to be protection scope of the present invention.
Claims (13)
- A Boletus aereus (Phlebopusportentosus) cultural method, comprise culture matrix preparation, inoculation bacteria, earthing, overburden layer cultural hypha, mushroom producing culture step, it is characterized in that described culture matrix comprises:Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏Red soil 10.0～20.0 ㎏Wheat 5.0～10.0 ㎏Rice bran 1.0～5.0 ㎏Calcium carbonate 1.0～2.0 ㎏MgSO410.0～20.0gKH2PO410.0～20.0gZnSO410.0～20.0gYeast diffusion juice 10.0～20.0gpH4.0～6.0。
- 2. according to the method for claim 1, it is characterized in that flame tree in described culture matrix, grapefruit, mango or other weedtree material need be ground into wood chip, chip dimensions 0.2cm～0.4cm × 1.0cm～1.5cm, prewet fermentation reactor system 30 days-60 days of wood chip; Prewet fermentation reactor system 60 days-90 days of wood chip, turning in every 30 days is once within the fermentation reactor system phase for wood chip and wood chip.
- 3. according to the method for claim 1 or 2, it is characterized in that culture vessel is 1400ml～1650ml plastic bottle.
- 4. according to the method for claim 1 or 2, it is characterized in that in the time that culture matrix is filled to culture vessel, after vessel port stays the space of 4 ㎝-5 ㎝ height to cover with matrix for mycelia, earthing is cultivated fruiting.
- 5. according to the method for claim 1 or 2, it is characterized in that inoculating bacteria and be selected from and connect liquid spawn and connect solid spawn.
- 6. according to the method for claim 1 or 2, it is characterized in that bacteria condition is: 27 DEG C～31 DEG C of temperature, CO２Concentration 800ppm-1500ppm, humidity 60%-75%, half-light, cultivates 30 days-50 days mycelia and covers with matrix.
- 7. according to the method for claim 1 or 2, it is characterized in that cover soil material is top layer 60 ㎝ red soils, top layer 30 ㎝ rubber garden mould or orchard soils, each bacterium bottle earthing 250g-300g, overburden layer thickness and bottleneck are flat.
- 8. according to the method for claim 7, it is characterized in that cover soil material further comprises and add 30%(volume ratio) wet turfy soil, soil moisture content 60%--70%.
- 9. according to the method for claim 1 or 2, it is characterized in that overburden layer cultural hypha condition is: 27 DEG C～31 DEG C of temperature, CO２Concentration 1000ppm-2000ppm, humidity 85%-90%, half-light, in incubation, artificial spraying keeps overburden layer surface wettability, cultivates 8 days-10 days mycelia and covers with overburden layer.
- 10. according to the method for claim 1 or 2, it is characterized in that mushroom producing culture condition is: 26 DEG C～30 DEG C of temperature, CO２Concentration 500ppm-1000ppm, humidity 90%-95%, with LED light source, illuminance 50LX-250LX, 8 hours every days of light application time, cultivates and within the 2nd day, starts fruiting, continue to cultivate 5 days-7 days, each blake bottle can a ripe 1-2 mushroom, in the time that mushroom lid does not launch completely, gathers in time.
- 11. according to the method for claim 1 or 2, it is characterized in that whole incubation completes in a culture vessel and without again transplanting.
- Cultivate the culture matrix of Boletus aereus for 12. 1 kinds, it comprises:Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏Flame tree, grapefruit, mango or other weedtree wood chips 5.0～10.0 ㎏Red soil 10.0～20.0 ㎏Wheat 5.0～10.0 ㎏Rice bran 1.0～5.0 ㎏Calcium carbonate 1.0～2.0 ㎏MgSO410.0～20.0gKH2PO410.0～20.0gZnSO410.0～20.0gYeast diffusion juice 10.0～20.0gpH4.0～6.0。
- 13. according to the culture matrix of claim 12, and in wherein said culture matrix, flame tree, grapefruit, mango or other weedtree material need be ground into wood chip, chip dimensions 0.2cm～0.4cm × 1.0cm～1.5cm, prewet fermentation reactor system 30 days-60 days of wood chip; Prewet fermentation reactor system 60 days-90 days of wood chip, turning in every 30 days is once within the fermentation reactor system phase for wood chip and wood chip.
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| CN104126416B (en) * | 2014-08-21 | 2016-03-30 | 石泉 | A kind of Boletus aereus merit domesticating method |
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Effective date of registration: 20180525 Address after: 562200 Guizhou Zhenfeng County, Southwest Guizhou Province, Buyi and Miao Autonomous Prefecture, min Valley Street, the two floor of landscape gold city. Patentee after: Guizhou Hong Zhen fungus industry investment development Co., Ltd. Address before: 200042 block A, 18 floor, Zhaofeng building, 369 Jiangsu Road, Shanghai. Patentee before: Shi Quan |
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Denomination of invention: A cultivation method of Boletus nigrum Effective date of registration: 20200929 Granted publication date: 20160504 Pledgee: ZHENFENG sub branch of Guiyang Bank Co., Ltd Pledgor: Guizhou Hong Zhen fungus industry investment development Co.,Ltd. Registration number: Y2020980006513 |