CN106105783A - A kind of batch production Boletus aereus cultural method - Google Patents
A kind of batch production Boletus aereus cultural method Download PDFInfo
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- CN106105783A CN106105783A CN201610618926.6A CN201610618926A CN106105783A CN 106105783 A CN106105783 A CN 106105783A CN 201610618926 A CN201610618926 A CN 201610618926A CN 106105783 A CN106105783 A CN 106105783A
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- mycelia
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- boletus aereus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The present invention relates to breed of edible fungus technical field, relate to a kind of batch production Boletus aereus cultural method.The present invention is in bottle cap central aperture, it is ensured that the aeration that cover soil material is good, makes mycelia normal growth；And conveniently supply moisture not enough during earthing, water holding capacity is strong, thoroughly inhibits bacteria phase bottleneck to climb fruiting morning that wall mycelia is formed, also reduces by the fruiting amount of 50%, and then reduce nutrient consumption, reduce and dredge mushroom cost of labor, it is ensured that the high yield of Boletus aereus.
Description
Technical field
The present invention relates to breed of edible fungus technical field, in particular to a kind of batch production Boletus aereus cultural method.
Background technology
Boletus aereus Phlebopus marginatus (Drum.ex Beak.) Wating&Greg is a kind of rare heat
Band edible fungi, is distributed mainly on Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia, New Zealand
And the ground such as Yunnan Province of China, Guangxi and Hainan.Boletus aereus quality is fresh and tender, delicious flavour, rich in nutritive value, high protein, low fat
Fat, rich in necessary mineral elements of human body such as 17 kinds of essential amino acids and abundant phosphorus, potassium, calcium, magnesium, ferrum and zinc, is relatively
Good health food also has good medical value, is deeply liked by consumer.
Although Boletus aereus may exit off the saprogenesis of host tree battalion, and used cultivation saprophytic bacteria method successful incubation
And achieve factory culture, but owing to it is saprophytic indifferent, than general saprophytic bacteria as Flammulina velutiper (Fr.) Sing, Auricularia, Pleurotus ostreatus, double spore
Mushrooms etc. are far short of what is expected.In the cultivation of Boletus aereus, either bag is planted or bottle is planted, although each bacterium bag or bacterium bottle can go out
A lot of mushroom flower bud, but can only grow and fully-developed 1 or 2 sporophore, and no matter sporophore quantity why, its gross weight is poor
Different not quite.
Due to the life habit that Boletus aereus is special, Boletus aereus factory culture there is problems in that 1. compost contains
The water yield is on the low side；2. fruiting area is big, fruiting quantity is too many, but the nutrition of Boletus aereus cultivation matrix the most enough supplies 1～2 son in fact
Body is grown up, and fruiting number affects finished product mushroom quality the most on the contrary；3. bacteria phase and sporophore growth phase draft loss compost and
Large quantity of moisture in cover soil material, water is the medium of nutrition transport, and in cultivation, moisture minimizing is i.e. the reduction of nutrition transport, enters
And have impact on sporophore growth, reduce single mushroom weight, have a strong impact on yield.Therefore in the case of meeting mycelia normal growth,
Keep compost and the water content of cover soil material to greatest extent, be to increase sporophore weight, the important measures of raising yield；4. support
What bacterium phase bottleneck grew climbs wall mycelia, forms early fruiting, consume nutrition and cause fruiting irregular, adding fertility after earthing
The difficulty of management.
In view of this, the special proposition present invention.
Summary of the invention
It is an object of the invention to provide a kind of Boletus aereus cultural method to solve the problems referred to above.
In order to realize the above-mentioned purpose of the present invention, spy by the following technical solutions:
A kind of Boletus aereus cultural method, comprises the following steps:
1), Boletus aereus strain is inoculated in the culture medium of bacterium bottle and carries out mycelia culture to mycelia and cover with described cultivation
Base；
2), to described media surface covering cover soil material and form overburden layer, the bottle mouth position at described bacterium bottle adds a cover bottle cap；
The mid portion perforate of described bottle cap；
3), to described overburden layer carrying out moisturizing, lucifuge is cultivated extremely described overburden layer and is covered with mycelia；
4), to mycelia carry out cultivation to make mycelia kink, form former base, grow to bottle cap tapping mushroom flower bud；
5), after dredging flower bud, carry out the cultivation of sporophore growth phase and gather.
Preferably, mycelia bottleneck of need not erasing after covering with training base is climbed wall mycelia and is directly carried out earthing, now cover soil material knot
Structure is loose makes machinery earthing run smooth and easy, and earthing and adding a cover is continuously finished by mechanized operation, to adapt to the effect of factory culture
Rate requirement.
Boletus aereus is again Phlebopus portentosus, and Classification system is Phlebopus marginatus (Drum.ex
Beak.) Wating&Greg, the cultural method that the present invention provides can suppress fruiting morning, minimizing fruiting quantity, increase culture medium
Utilization rate, and be conducive to the moisturizing during cultivation.
Concrete, prior art does not the most add a cover bottle cap, and the present invention adds a cover rear bottle cap and is closely together with bottleneck, in bottle cap
Between perforate, be convenient to keep humidity, the evaporation capacity on the one hand adding a cover rear moisture to greatly reduce, on the other hand moisture is in the middle of bottle cap
Tapping penetrates into overburden layer, and cover soil material water content greatly improves, and makes mycelia vigorous in the optimum cover soil material of water content
Growth；
Additionally, the oxygen that the part of bottle cap perforate has abundance makes mycelia normal growth and fruiting, and the portion covered by bottle cap
Divide mycelia that kink or can not be difficult to be divided into former base and form sporophore, thoroughly inhibit bacteria phase bottleneck to climb what wall mycelia was formed
Early fruiting；
Fruiting only at bottle cap tapping, remaining charge level all can not fruiting, decrease fruiting area, thus decrease fruiting
Amount, improves the nutrient utilization of advantage mushroom, decreases insignificant nutrient consumption, also reduces and dredge mushroom cost of labor.
Mushroom flower bud is many to be grown in soil under overburden layer surface, and fruiting is neat, affected by environment little.
Preferably, Boletus aereus cultural method described above, in step 1) in, the condition of culture bag of described mycelia culture
Include:
Temperature 25 DEG C～30 DEG C, air humidity 50%～80%, CO2Concentration 1500ppm～3000ppm.Train under the conditions of this
Support 35～40 days mycelia and cover with culture medium；
The formula of described culture medium includes following component in terms of parts by weight: wood flour and/or wood chip 40～60 parts, red soil 15
～25 parts, wheat berry 5～15 parts, millet 5～15 parts, Testa oryzae 5～11 parts, Semen Maydis powder 5～15 parts, Gypsum Fibrosum powder 1～3 parts, MgSO4
0.1～0.3 part, KH2PO40.1～0.3 part, ZnSO41～3 part.
The earthing stage can be entered.
Preferably, Boletus aereus cultural method described above, in step 2) in, the water content of described cover soil material is 30%
～50%, the thickness of described overburden layer is 2cm～5cm.
Being more highly preferred to, cover soil material of the present invention is selected from the patent application literary composition of application number 201110355384.5
The cover soil material provided in part embodiment 2, the date of publication of this patent application document is 2012.05.30.
Preferably, Boletus aereus cultural method described above, in step 2) in, the diameter of the mid portion institute perforate of bottle cap
For 4cm～6cm, reduce fruiting area 80%～56%.
Bacterium bottle used by the present invention is 1400ml～1800ml bacterium bottle, bottleneck diameter 9.0cm, bottleneck area 63.58cm2Bottle
Lid opening diameter is 4.0cm～6.0cm, orifice area 19.68cm2～28.26cm2, this perforate directly decreases 80%～56%
Fruiting area, thus reduce by the fruiting amount of 80%～56%.And under this opening diameter, the regulation of ventilation and humidity is the most more
Suitably.
Preferably, Boletus aereus cultural method described above, in step 3) in, before described overburden layer is carried out moisturizing
Also include:
In described bacterium bottle surface lid layer of non-woven fabric as moisturizing layer.
Non-woven fabrics is a kind of non-weaving cloth, directly utilizes high polymer section, chopped fiber or long filament by various web formation
What method and concretion technology were formed has softness, the ventilative and tencel goods of planar structure, and its feature is not produce fibre
Dimension bits are tough, durable.Non-woven fabrics has extraordinary performance of keeping humidity when using.
Preferably, Boletus aereus cultural method described above, in step 3) in, the concrete operations of described moisturizing are:
At the surface sprinkling of described moisturizing layer, keep described overburden layer humidity 50%～65%, air humidity 90%～
98%.
During cultivating overburden layer mycelial growth after adding a cover, every day sprays water 1 time at bacterium bottle surface, and moisture is from bottle cap central aperture
Bottle inner periphery soil is penetrated at place, keeps cover soil material optimum water content 50%～55% to cultivate overburden layer mycelia vigorous growth.
Cultivating 7～8 days after general earthing, mycelia can cover with overburden layer, and without fruiting morning.
Preferably, Boletus aereus cultural method described above, in step 4) in, described cultivation mycelia to bottle cap is opened
The condition of culture that at hole, mushroom flower bud grows includes:
CO2Concentration 1000ppm～3000ppm, temperature 25 DEG C～30 DEG C, overburden layer humidity 50%～65%, space humidity
90%～98%, illuminance 200lx～600lx, light application time 8h～10h.
This culture period specifically refers to mycelia and covers with after overburden layer to during Slex.
Preferably, Boletus aereus cultural method described above, in step 5) in, described thin flower bud operation particularly as follows:
Remove unnecessary mushroom flower bud when described mushroom flower bud length to bacteria cover diameter 1cm～3cm, thick 1cm～2cm of handle, stay 1～2
Mushroom flower bud.
Preferably, Boletus aereus cultural method described above, in step 5) in, the cultivation that the described sporophore growth phase cultivates
Condition includes:
CO2Concentration 800ppm～2000ppm, temperature 24 DEG C～29 DEG C, overburden layer humidity 50%～65%, space humidity
80%～95%, illuminance 200lx～600lx, light application time 8h～10h；
Stop water spray and increase ventilation.
Owing to adding a cover bottle cap after earthing of the present invention, reduce the charge level area being exposed in air, reduce water evaporation quantity, from
And maintaining compost and cover soil material water content to greatest extent, the sporophore growth phase mends to cultivation matrix and cover soil material
Water.In the prior art, the sporophore growth phase is sprayed onto mushroom surface quilt to moisture while compost and cover soil material moisturizing
Mushroom body absorbs, and makes cap blackening, color burn, mushroom body water content increase, and mushroom handle foams, hollow, and the soft not consolidation of meat is fresh-keeping
Phase short (1～2 day), can only at source sell, it is impossible to long distance transportation.
Preferably, Boletus aereus cultural method described above, in step 5) in, described in gather and reach in sporophore Maturity
Carry out when 75%～80%.
When sporophore cap is open and flat, lid edge stretches or slightly rolls up, tube olive-drab, and Maturity reaches when 75%～80% timely
Gathering, the whole handle base that involves in a criminal case is pulled up, and now sporophore meat consolidation good springiness, handle are firmly, average weight 90g～110g.
Compared with prior art, the invention have the benefit that
1), the present invention in bottle cap central aperture, at least have the advantage that
Ensure the aeration that cover soil material is good, make mycelia normal growth；
Moisture not enough when being convenient to supply earthing, cover soil material water content improve to 50% from 40%～45%～
65%, make mycelia vigorous growth in the optimum cover soil material of water content；And owing to water holding capacity is strong, the sporophore growth phase is not
Cultivation matrix and cover soil material moisturizing, the bolete good mushroom type turned out, the shallow olive-drab of mushroom body, cap surface quilt is given with extra
Powder, color are vivid, stem is hard, meat consolidation, mushroom body water content low (water content 85%～87%), shelf-stable (4 DEG C～8
Can store 6-8 days under the conditions of DEG C freezer)；
Aperture segment has the oxygen of abundance to make mycelia normal growth and fruiting, the part mycelia covered by bottle cap can not or difficult
It is divided into former base in kink and forms sporophore, thoroughly inhibiting bacteria phase bottleneck to climb fruiting morning that wall mycelia is formed；
Fruiting at tapping, remaining charge level all can not fruiting, decrease the fruiting area of 56%～80%, thus reduce
The fruiting amount of 56%～80%, and then reduce nutrient consumption, reduce and dredge mushroom cost of labor.
2), mushroom flower bud is many grows in soil under overburden layer surface, and fruiting is neat, affected by environment little.
3) condition of culture of each culture period, is defined, it is ensured that the high yield of Boletus aereus.
Detailed description of the invention
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but those skilled in the art will
Understanding, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.In embodiment unreceipted specifically
Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or instrument unreceipted production firm person, be
Can be by the commercially available conventional products bought and obtain.
Embodiment 1
A kind of Boletus aereus cultural method, comprises the following steps:
1), inoculation bacteria step: the most cultured Boletus aereus strain is inoculated into sterilized bottled culture medium
In matter, at temperature 25 DEG C, humidity 50%, CO2Carry out mycelia culture under conditions of concentration 1500ppm, cultivate 40 days under the conditions of this
Mycelia covers with culture matrix, can enter the earthing stage.
The formula of described culture medium is in terms of parts by weight, including following component: wood flour 40 parts, 25 parts of red soil, wheat berry 15
Part, 5 parts of millet, 11 parts of Testa oryzae, Semen Maydis powder 5 parts, Gypsum Fibrosum powder 1 part, MgSO40.1 part, KH2PO40.3 part, ZnSO41 part.
2), earthing step: cover cover soil material on the surface of the culture medium covering with mycelia, form overburden layer, earthing material
The water content of material is 30%, and earthing thickness 2cm adds a cover bottle cap at bottleneck subsequently, the mid portion perforate of bottle cap, bore dia 4cm,
Reduce by the fruiting area of 80%, and then reduce by the fruiting amount of 80%；
Earthing, added a cover after enter mushroom room cultivate bacterium overburden layer mycelial growth, bacterium bottle surface spray water, allow moisture from
Slowly penetrate surrounding soil at bottle cap central aperture, keep overburden layer humidity 50%, air humidity 90%, CO2Concentration
2000ppm, lucifuge, cultivate 6 days mycelia and cover with overburden layer, can enter and urge former base formed and urge the flower bud stage.
3), fertility step:
1. after mycelia covers with overburden layer, at CO2Concentration 1000ppm, temperature 25 DEG C, space humidity 95%, illuminance 200lx
Cultivating 3 days, light application time 10h, mycelia is twisted together and forms a large amount of former base；
2. flower bud is urged: after former base is formed, at CO2Concentration 1000ppm, temperature 26 DEG C, space humidity 95%, illuminance 300lx,
Light application time 10h, urges flower bud to cultivate 2 days, and the top of former base differentiates cap, forms children flower bud, cultivating rate 98%；
3. dredge flower bud: sporophore bacteria cover diameter be 1cm, handle thick 1cm time remove unnecessary mushroom flower bud, 1～2 mushroom can be stayed
Flower bud；
4. sporophore growth: after dredging flower bud, reduces CO2To 800ppm, temperature is down to 25 DEG C, controls space humidity 80%, light
Illumination 200lx, light application time 10h, keep compost and cover soil material water content 50%, increase ventilation simultaneously, promote nutrition
Absorb from compost and be delivered up to sporophore aerial parts, increase sporophore weight；
5. gathering: when sporophore cap is open and flat, lid edge stretches or slightly rolls up, tube olive-drab, when Maturity reaches 75% and
Shi Caishou, the whole handle base that involves in a criminal case pulls up, and now sporophore meat consolidation good springiness, handle are hard.
Embodiment 2
1), inoculation bacteria step: Boletus aereus strain is inoculated in sterilized bottled culture medium, in temperature 28
DEG C, humidity 70%, CO2Concentration 2500ppm carries out mycelia culture, cultivates 38 days mycelia and covers with culture matrix, can enter under the conditions of this
Enter the earthing stage.
The formula of described culture medium is in terms of parts by weight, including following component: wood chip 60 parts, 15 parts of red soil, wheat berry 5
Part, 15 parts of millet, 5 parts of Testa oryzae, Semen Maydis powder 15 parts, Gypsum Fibrosum powder 3 parts, MgSO40.3 part, KH2PO40.1 part, ZnSO43 parts.
2), earthing step: cover cover soil material on the surface of the culture medium covering with mycelia, form overburden layer, earthing material
The water content of material is 42.5%, and earthing thickness 3cm adds a cover bottle cap at bottleneck subsequently, the mid portion perforate of bottle cap, bore dia
5cm, earthing, added a cover after enter mushroom room, bacterium bottle surface spray water, allow moisture slowly penetrate week at bottle cap central aperture
Enclose soil, keep overburden layer humidity 60%, air humidity 95%, lucifuge, cultivate 7 days overburden layer mycelia and cover with.
3), fertility step is:
1. after mycelia covers with overburden layer, at CO2Concentration 2000ppm, temperature 28 DEG C, space humidity 95%, illuminance
250lx, light application time 8h, to cultivate 2 days, mycelia is twisted together and forms a large amount of former base；
2. flower bud is urged: after former base is formed, at CO2Concentration 1500ppm, temperature 27 DEG C～29 DEG C, space humidity 97%, illuminance
400lx, light application time 8h, urge flower bud to cultivate 1 day, the top of former base differentiates cap, forms children flower bud, cultivating rate 98%；
3. dredge flower bud: sporophore bacteria cover diameter be 2cm, handle thick 1.5cm time remove unnecessary mushroom flower bud, 1 mushroom flower bud can be stayed；
4. sporophore growth: after dredging flower bud, reduces CO2To 1500ppm, temperature is down to 27 DEG C, controls space humidity 90%, light
Illumination 300lx, light application time 9h, keep compost and cover soil material water content 55%, increase ventilation simultaneously, promote that nutrition is certainly
Compost absorbs and is delivered up to sporophore aerial parts, increases sporophore weight；
5. gathering: when sporophore cap is open and flat, lid edge stretches or slightly rolls up, tube olive-drab, when Maturity reaches 78% and
Shi Caishou, the whole handle base that involves in a criminal case pulls up, and now sporophore meat consolidation good springiness, handle are hard, the average mushroom weight 110g that every bottle is gathered.
Embodiment 3
1), inoculation bacteria step: Boletus aereus strain is inoculated in sterilized bottled culture medium, in temperature 30
DEG C, humidity 80%, CO2Concentration 3000ppm carries out mycelia culture, cultivates 40 days mycelia and covers with culture matrix, can enter under the conditions of this
Enter the earthing stage.
The formula of described culture medium includes following component in terms of parts by weight: wood flour 20 parts, wood chip 30 parts, 20 parts of red soil,
Wheat berry 10 parts, 10 parts of millet, 7 parts of Testa oryzae, Semen Maydis powder 10 parts, Gypsum Fibrosum powder 2 parts, MgSO40.2 part, KH2PO40.2 part,
ZnSO42 parts.
2), earthing step is: covers cover soil material on the surface of the culture medium covering with mycelia, forms overburden layer, earthing
The water content of material is 50%, and earthing thickness 5cm adds a cover bottle cap at bottleneck subsequently, the mid portion perforate of bottle cap, bore dia
6cm, earthing, added a cover after enter mushroom room, bacterium bottle surface spray water, allow moisture slowly penetrate week at bottle cap central aperture
Enclose soil, keep overburden layer humidity 65%, air humidity 98%, lucifuge, cultivate 8 days overburden layer mycelia and cover with.
3), fertility step is:
1. after mycelia covers with overburden layer, at CO2Concentration 3000ppm, temperature 30 DEG C, space humidity 98%, illuminance
600lx, light application time 8h, to cultivate 2 days, mycelia is twisted together and forms a large amount of former base；
2. flower bud is urged: after former base is formed, at CO2Concentration 2500ppm, temperature 30 DEG C, space humidity 98%, illuminance 400lx,
Light application time 8h, urges flower bud to cultivate 2 days, and the top of former base differentiates cap, forms children flower bud, cultivating rate 98%～100%；
3. dredge flower bud: sporophore bacteria cover diameter be 3cm, handle thick 2cm time remove unnecessary mushroom flower bud, 1 mushroom flower bud can be stayed；
4. sporophore growth: after dredging flower bud, reduces CO2To 2000ppm, temperature is down to 29 DEG C, controls space humidity 95%, light
Illumination 600lx, light application time 8h, keep compost and cover soil material water content 65%, increase ventilation simultaneously, increase sporophore
Transpiration, promotes that nutrition absorbs from compost and is delivered up to sporophore aerial parts, increase sporophore weight, grow up 1
Sporophore；
5. gathering: when sporophore cap is open and flat, lid edge stretches or slightly rolls up, tube olive-drab, when Maturity reaches 80% and
Shi Caishou, the whole handle base that involves in a criminal case pulls up, and now sporophore meat consolidation good springiness, handle are hard, the average mushroom weight 115g that every bottle is gathered.
Embodiment 4
Operating consistent with embodiment 3, difference is when dredging flower bud to stay 2 mushroom flower buds, obtains 2 sporophore after growing up.
Experimental example
For the effect of the present invention is better described, comparative example 1～3 is set on the basis of embodiment 2 and compares.
Reference examples 1
Inoculation bacteria step, earthing step, fertility step are basically identical with embodiment 2, and difference is: cover soil material
Water content be 40%, earthing thickness 4cm, overburden layer surface exposure in air, overburden layer mycelia culture phase moisturizing every day 1 time
Increase soil moisture content is to 60%, and after dredging flower bud, every day sporophore growth phase sprays water 1 time and dissipates because ventilating with supplementary overburden layer and substrate
The moisture lost.
Reference examples 2
Inoculation bacteria step, earthing step, fertility step are basically identical with embodiment 2, and difference is: cover soil material
Water content be 45%, earthing thickness 4cm, overburden layer surface exposure in air, overburden layer mycelia culture phase moisturizing every day 1 time
Increase soil moisture content is to 60%, and after dredging flower bud, every day sporophore growth phase sprays water 1 time and dissipates because ventilating with supplementary overburden layer and substrate
The moisture lost.
Reference examples 3
Inoculation bacteria step, earthing step, fertility step are basically identical with embodiment 2, and difference is: cover soil material
Water content be 50%, earthing thickness 4cm, overburden layer surface exposure in air, overburden layer mycelia culture phase moisturizing every day 1 time
Increase soil moisture content is to 60%, and after dredging flower bud, every day sporophore growth phase sprays water 1 time and dissipates because ventilating with supplementary overburden layer and substrate
The moisture lost.
Observed by cultivation and record, compare embodiment 1～4 and reference examples 1～3 Boletus aereus factory culture side
Method, when mycelia covers with overburden layer time, overburden layer mycelium growth vigor, mycelia kink regularity, former base formation amount, earthing to fruiting
Between, fruiting regularity, fruiting percentage rate, collection period, average every bottle entity weight, mushroom quality, earthing be to end time of gathering, mushroom
The difference of 13 cultivated characters such as body water content, freshness date, describes embodiment 1～4 and the difference of reference examples 1～3 cultivated character
Different (being shown in Table 1).As can be seen from the table, in 13 described cultivated characters, after earthing, the method for adding a cover is superior to earthing and is not added with
Lid, overburden layer surface exposure is in air.
Table 1 embodiment and reference examples cultivated character compare
As known from Table 1, the Boletus aereus cultural method that the application provides is used, when can significantly shorten the cultivation of Boletus aereus
Between, and fruiting steam, cultivating rate is high.And owing to water holding capacity is strong, the sporophore growth phase need not additionally give cultivation matrix and earthing
Material moisturizing, the bolete good mushroom type turned out, the shallow olive-drab of mushroom body, cap surface are vivid by powder, color, stem
Firmly, meat consolidation, mushroom body water content low (water content 85%～87%), shelf-stable (can store 6-8 under the conditions of 4 DEG C～8 DEG C of freezers
My god).
Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that without departing substantially from the present invention's
May be made that in the case of spirit and scope many other change and amendment.It is, therefore, intended that in the following claims
Including all such changes and modifications belonged in the scope of the invention.
Claims (10)
1. a Boletus aereus cultural method, it is characterised in that comprise the following steps:
1), Boletus aereus strain is inoculated in the culture medium of bacterium bottle and carries out mycelia culture to mycelia and cover with described culture medium；
2), to described media surface covering cover soil material and form overburden layer, the bottle mouth position at described bacterium bottle adds a cover bottle cap；Described
The mid portion perforate of bottle cap；
3), to described overburden layer carrying out moisturizing, lucifuge is cultivated extremely described overburden layer and is covered with mycelia；
4), to mycelia carry out cultivation to make mycelia kink, form former base, grow to bottle cap tapping mushroom flower bud；
5), after dredging flower bud, carry out the cultivation of sporophore growth phase and gather.
Boletus aereus cultural method the most according to claim 1, it is characterised in that in step 1) in, described mycelia culture
Condition of culture includes:
Temperature 25 DEG C～30 DEG C, air humidity 50%～80%, CO2Concentration 1500ppm～3000ppm.
Boletus aereus cultural method the most according to claim 1, it is characterised in that in step 2) in, described cover soil material
Water content is 30%～50%, and the thickness of described overburden layer is 2cm～5cm.
Boletus aereus cultural method the most according to claim 1, it is characterised in that in step 2) in, the mid portion of bottle cap
A diameter of 4cm～6cm of institute's perforate.
Boletus aereus cultural method the most according to claim 1, it is characterised in that in step 3) in, to described overburden layer
Also include before carrying out moisturizing:
In described bacterium bottle surface lid layer of non-woven fabric as moisturizing layer.
Boletus aereus cultural method the most according to claim 5, it is characterised in that in step 3) in, described moisturizing concrete
Operation is:
At the surface sprinkling of described moisturizing layer, keep described overburden layer humidity 50%～65%, air humidity 90%～98%.
Boletus aereus cultural method the most according to claim 1, it is characterised in that in step 4) in, described mycelia is carried out
Cultivate the condition of culture grown to bottle cap tapping mushroom flower bud to include:
CO2Concentration 1000ppm～3000ppm, temperature 25 DEG C～30 DEG C, overburden layer humidity 50%～65%, space humidity 90%～
98%, illuminance 200lx～600lx, light application time 8h～10h.
Boletus aereus cultural method the most according to claim 1, it is characterised in that in step 5) in, described thin flower bud operation tool
Body is:
Remove unnecessary mushroom flower bud when described mushroom flower bud length to bacteria cover diameter 1cm～3cm, thick 1cm～2cm of handle, stay 1～2 mushroom flower bud.
Boletus aereus cultural method the most according to claim 1, it is characterised in that in step 5) in, described sporophore growth
The condition of culture that phase cultivates includes:
CO2Concentration 800ppm～2000ppm, temperature 24 DEG C～29 DEG C, overburden layer humidity 50%～65%, space humidity 80%～
95%, illuminance 200lx～600lx, light application time 8h～10h；
Stop water spray and increase ventilation.
10. according to Boletus aereus cultural method described in any one of claim 1～9, it is characterised in that in step 5) in, described
Gather and carry out when sporophore Maturity reaches 75%～80%.
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| CN109089730A (en) * | 2018-09-07 | 2018-12-28 | 景洪宏臻农业科技有限公司 | A kind of cultivation Boletus aereus earthing method |
| CN109122051A (en) * | 2018-08-31 | 2019-01-04 | 景洪宏臻农业科技有限公司 | A kind of cultural method of Boletus aereus solid spawn |
| CN117025415A (en) * | 2023-08-17 | 2023-11-10 | 景洪宏臻农业科技有限公司 | Phlebopus portentosus strain V239.04 and application thereof |
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