CN103880539A - Boletus aereus culturing base stock and preparation method thereof - Google Patents

Boletus aereus culturing base stock and preparation method thereof Download PDF

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Publication number
CN103880539A
CN103880539A CN201410115075.4A CN201410115075A CN103880539A CN 103880539 A CN103880539 A CN 103880539A CN 201410115075 A CN201410115075 A CN 201410115075A CN 103880539 A CN103880539 A CN 103880539A
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boletus aereus
culture base
preparation
parts
base stock
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CN201410115075.4A
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葛静波
陈春化
王浩东
沈业庭
季林章
孙晓亮
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SIYANG COUNTY INSTITUTE OF AGRICULTURAL SCIENCES
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SIYANG COUNTY INSTITUTE OF AGRICULTURAL SCIENCES
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Abstract

The invention provides a boletus aereus culturing base stock and a preparation method thereof, and relates to the technical field of edible fungi cultivation. The culturing base stock is prepared from the following components: edible fungi slag, humus soil, dried cow dung, honeycomb, pine needle, wheat bran, monopotassium phosphate, forchlorfenuron, calcium carbonate and quicklime. The preparation method of the culturing base stock comprises the following steps: (1) preparing materials; (2) mixing; (3) fermenting; (4) packaging. According to the boletus aereus culturing base stock and the preparation method thereof, resource recycling can be realized on the premise that the nutritional requirement for the growth of boletus aereus is met, the cost for the culturing base stock is reduced, and the benefit is increased; according to boletus aereus cultured by utilizing the culturing base stock, the fruiting period can be prolonged, the yield is increased, and the content of partial nutritional elements is broken through.

Description

Culture base-material of a kind of Boletus aereus and preparation method thereof
Technical field
The present invention relates to the preparation method of culture base-material He this culture base-material in a kind of fungus growing technique field, particularly a kind of Boletus aereus.
Background technology
Boletus aereus, belongs to the one of bolete, and main another name has big foot mushroom, fries bacterium, also claims thigh mushroom, reticulate pattern bolete, belongs to Mycophyta.The flat semisphere of Boletus aereus cap, outside and bacterial context are all black, and diameter is larger, generally can reach 20cm left and right, and the quality of single Boletus aereus can reach 500-800g, and cap is thicker, has many apertures below, similar with beef liver, therefore named Boletus aereus.Boletus aereus delicious flavour is one of the most fragrant bolete.And Boletus aereus is nutritious, contain a large amount of protein and 18 seed amino acids, there is the effects such as anti-cancer, cough-relieving, tonifying Qi, be fat-reducing edible mushrooms, to hypertension, the diseases such as hypercholesterolemia and hyperlipidemia have good effect.The distinctive bolete kind of Zhe Yeshi China.
Along with in recent years, people are for the pursuit of green health, and bolete obtains people's high praise, and wherein the demand of Boletus aereus increases especially day by day.This causes wild Boletus aereus quantity sharply to decline, and the marketable value of Boletus aereus is improved constantly, and has now become one of comparatively expensive edible fungus variety.Because the history of China's artificial culture Boletus aereus is not remote, tame Boletus aereus output is not still well positioned to meet people's demand, is the comparatively harsh for growing environment of Boletus aereus on the one hand; The sporophore that is on the other hand Boletus aereus is larger, easily grows other diseases, and cultivation needs larger space and the management of science.
For front one side, existing artificial culture culture base-material, adopt the agricultural byproducts such as wood chip, rice bran is major ingredient more, comparatively limits to for the selection of other material.The cultivation of some similar wild-type strains has all broken through the selection of material, can accomplish to gather materials on the spot, and resource re-uses, and reduces costs, and increases benefit.The cultivation of Boletus aereus also can break through the material selection of existing culture base-material so, accomplishes to reduce costs, and ensures Boletus aereus normal growth simultaneously.
Summary of the invention
Goal of the invention: for above-mentioned deficiency, overcome the defect of prior art, the object of this invention is to provide a kind of preparation method of culture base-material He this culture base-material of Boletus aereus.
A culture base-material for Boletus aereus, is made up of the component of following mass fraction:
Edible fungi residue 120-200 part, vegetable mould 50-85 part, dry cow dung 22-40 part, honeycomb 3-20 part, pine needle 40-75 part, wheat bran 35-65 part, potassium primary phosphate 15-25 part, forchlorfenuron 0.5-1.5 part, calcium carbonate 30-60 part and unslaked lime 35-85 part.
As preferably, edible fungi residue is golden mushroom slag or Pleurotus eryngii bacterium slag.
As preferably, the mass fraction of honeycomb is 5-15 part.
A preparation method for the culture base-material of Boletus aereus, comprises the following steps:
(1) get the raw materials ready: edible fungi residue 120-200 part, vegetable mould 50-85 part, dry cow dung 22-40 part, honeycomb 3-20 part, pine needle 40-75 part, wheat bran 35-65 part, potassium primary phosphate 15-25 part, forchlorfenuron 0.5-1.5 part, calcium carbonate 30-60 part and unslaked lime 35-85 part;
(2) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keep the water content of mixture between 45%-65%, pH value 5.2-5.9;
(3) fermentation: after stirring, be 15-25 DEG C in temperature, the environment bottom fermentation of relative air humidity 65%-75%;
(4) pack: after thing fermentation ends to be mixed, mixture is packed in bacterium bag, sealing, makes.
As preferably, the fermentation time of fermentation stage is 15-35 day.
As preferably, bacterium bag is vinyon bacterium bag.
Beneficial effect: culture base-material of Boletus aereus provided by the invention and preparation method thereof, breaking through the material of existing culture base-material selects, utilize multiple material, comprise edible fungi residue, ight soil, pine needle and honeycomb, under the interaction of above-mentioned materials, not only meet the Boletus aereus necessary nutritional needs of growing, also increase the comprehensive of Boletus aereus nutrition, especially edible fungi residue and honeycomb, produces fabulous nutritional support is provided for the stalwartness of Boletus aereus mycelia.
Culture base-material of Boletus aereus provided by the invention and preparation method thereof, under the prerequisite of nutritional needs that meets Boletus aereus growth, accomplishes the re-using of resource, has reduced the cost of culture base-material, has increased benefit.Utilize this culture base-material cultivation Boletus aereus not only to extend the fruiting phase, increased output, and obtained breakthrough on the content of part nutritive element.
Embodiment
In order further to understand the present invention, below in conjunction with embodiment, the preferred embodiment of the invention is described, but should be appreciated that these are described is for further illustrating the features and advantages of the present invention, instead of limiting to the claimed invention.
Embodiment 1
A culture base-material for Boletus aereus, is made up of the component of following mass fraction:
35 parts of 120 parts of golden mushroom slags, 50 parts of vegetable mould, 22 parts of dry cow dungs, 5 parts, honeycomb, 40 parts of pine needles, 35 parts, wheat bran, 15 parts of potassium primary phosphates, 0.5 part of forchlorfenuron, 30 parts, calcium carbonate and unslaked limes.
A preparation method for the culture base-material of Boletus aereus, comprises the following steps:
(1) get the raw materials ready: 35 parts of 120 parts of golden mushroom slags, 50 parts of vegetable mould, 22 parts of dry cow dungs, 5 parts, honeycomb, 40 parts of pine needles, 35 parts, wheat bran, 15 parts of potassium primary phosphates, 0.5 part of forchlorfenuron, 30 parts, calcium carbonate and unslaked limes;
(2) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keeping the water content of mixture is 55%, pH value 5.4;
(3) fermentation: after stirring, be 15 DEG C in temperature, the environment bottom fermentation of relative air humidity 70%, fermentation time is 25;
(4) pack: after thing fermentation ends to be mixed, mixture is packed in vinyon bacterium bag, sealing, makes.
Embodiment 2
A culture base-material for Boletus aereus, is made up of the component of following mass fraction:
85 parts of 200 parts of golden mushroom slags, 85 parts of vegetable mould, 40 parts of dry cow dungs, 15 parts, honeycomb, 75 parts of pine needles, 65 parts, wheat bran, 25 parts of potassium primary phosphates, 1.5 parts of forchlorfenurons, 60 parts, calcium carbonate and unslaked limes.
A preparation method for the culture base-material of Boletus aereus, comprises the following steps:
(1) get the raw materials ready: 85 parts of 200 parts of golden mushroom slags, 85 parts of vegetable mould, 40 parts of dry cow dungs, 15 parts, honeycomb, 75 parts of pine needles, 65 parts, wheat bran, 25 parts of potassium primary phosphates, 1.5 parts of forchlorfenurons, 60 parts, calcium carbonate and unslaked limes;
(2) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keeping the water content of mixture is 56%, pH value 5.5;
(3) fermentation: after stirring, be 15 DEG C in temperature, the environment bottom fermentation of relative air humidity 70%, fermentation time is 30;
(4) pack: after thing fermentation ends to be mixed, mixture is packed in vinyon bacterium bag, sealing, makes.
Embodiment 3
A culture base-material for Boletus aereus, is made up of the component of following mass fraction:
77 parts of 165 parts of Pleurotus eryngii bacterium slags, 75 parts of vegetable mould, 32 parts of dry cow dungs, 11 parts, honeycomb, 65 parts of pine needles, 55 parts, wheat bran, 20 parts of potassium primary phosphates, 1 part of forchlorfenuron, carbonic acid Ca45 part and unslaked limes.
A preparation method for the culture base-material of Boletus aereus, comprises the following steps:
(1) get the raw materials ready: 77 parts of 165 parts of Pleurotus eryngii bacterium slags, 75 parts of vegetable mould, 32 parts of dry cow dungs, 11 parts, honeycomb, 65 parts of pine needles, 55 parts, wheat bran, 20 parts of potassium primary phosphates, 1 part of forchlorfenuron, carbonic acid Ca45 part and unslaked limes;
(2) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keeping the water content of mixture is 60%, pH value 5.3;
(3) fermentation: after stirring, be 15 DEG C in temperature, the environment bottom fermentation of relative air humidity 70%, fermentation time is 28;
(4) pack: after thing fermentation ends to be mixed, mixture is packed in vinyon bacterium bag, sealing, makes.
Utilizing the culture base-material of Boletus aereus cultivation conventional in prior art is control group, prepares culture base-material taking weed tree sawdust and Semen Maydis powder as major ingredient, is control group 1.Embodiment 1-3 and control group 1 are carried out to Boletus aereus experiment in cultivation.In test, indoor temperature, humidity, illumination, ventilation management work and earthing condition are identical, observation fruiting time, fruiting phase and output.The Boletus aereus afterwards embodiment group and control group being cultivated carries out Analysis of Nutritional test.
Test-results is as follows:
(1) comparative analysis of fruiting time, fruiting phase and output.
The culture base-material control group of embodiment group and prior art, during budding fruiting time, fruiting and output have certain difference.
The fruiting time average of buddingging of embodiment group is done sth. in advance 1.5 days; Stop the fruiting time upper, embodiment group is on average later than control group 2.5 days, and the whole fruiting phase on average extends 4 days; In output, single sporophore weight embodiment group is than control group average weight gain 1.4%, and on yield per unit, embodiment group on average increases production 4.7% than control group.
(2) difference of the Boletus aereus main nutrient composition of different culture media material cultivation, result is as shown in table 1:
The main nutrient composition content (unit :/100g dry weight) of table 1 different culture media material cultivation Boletus aereus
Figure DEST_PATH_IMAGE002A
As seen from table, the content of the carbohydrate of embodiment group, protein, ash content and zinc is higher than control group, and lipid content is lower than control group; Content and the control group of other nutritive ingredients remain basically stable.Visible, the nutritive value of embodiment group is higher, and the low demand that more can meet people of lipid content.
In summary, the culture base-material that the culture base-material preparation method of Boletus aereus provided by the invention makes, not only can impel Boletus aereus to shift to an earlier date fruiting compared to prior art, extend the fruiting phase, and on part nutritive value higher than existing culture base-material.
To the above-mentioned explanation of the disclosed embodiments, make professional and technical personnel in the field can realize or use the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiment, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.

Claims (6)

1. a culture base-material for Boletus aereus, is characterized in that being made up of the component of following mass fraction:
Edible fungi residue 120-200 part, vegetable mould 50-85 part, dry cow dung 22-40 part, honeycomb 3-20 part, pine needle 40-75 part, wheat bran 35-65 part, potassium primary phosphate 15-25 part, forchlorfenuron 0.5-1.5 part, calcium carbonate 30-60 part and unslaked lime 35-85 part.
2. the culture base-material of Boletus aereus according to claim 1, is characterized in that: edible fungi residue is golden mushroom slag or Pleurotus eryngii bacterium slag.
3. the culture base-material of Boletus aereus according to claim 1, is characterized in that: the mass fraction of honeycomb is 5-15 part.
4. a preparation method for the culture base-material of Boletus aereus claimed in claim 1, is characterized in that: comprise the following steps:
(1) get the raw materials ready: edible fungi residue 120-200 part, vegetable mould 50-85 part, dry cow dung 22-40 part, honeycomb 3-20 part, pine needle 40-75 part, wheat bran 35-65 part, potassium primary phosphate 15-25 part, forchlorfenuron 0.5-1.5 part, calcium carbonate 30-60 part and unslaked lime 35-85 part;
(2) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keep the water content of mixture between 45%-65%, pH value 5.2-5.9;
(3) fermentation: after stirring, be 15-25 DEG C in temperature, the environment bottom fermentation of relative air humidity 65%-75%;
(4) pack: after thing fermentation ends to be mixed, mixture is packed in bacterium bag, sealing, makes.
5. the preparation method of the culture base-material of Boletus aereus according to claim 4, is characterized in that: the fermentation time of fermentation stage is 15-35 day.
6. the preparation method of the culture base-material of Boletus aereus according to claim 4, is characterized in that: bacterium bag is vinyon bacterium bag.
CN201410115075.4A 2014-03-26 2014-03-26 Boletus aereus culturing base stock and preparation method thereof Pending CN103880539A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106105783A (en) * 2016-08-01 2016-11-16 景洪宏臻农业科技有限公司 A kind of batch production Boletus aereus cultural method
CN106866205A (en) * 2017-01-24 2017-06-20 中国科学院昆明植物研究所 A kind of stropharia rugoso-annulata plantation matrix and preparation method thereof
CN106866210A (en) * 2017-03-09 2017-06-20 海门市利欣农产品有限公司 A kind of bolete culture base-material and preparation method thereof
CN109392593A (en) * 2018-12-03 2019-03-01 贵州家鑫食用菌有限公司 A method of beefsteak mushroom is planted using Pleurotus eryngii dead meal
CN112400610A (en) * 2020-11-27 2021-02-26 贵州百居关农旅开发有限公司 Culture medium for boletus and preparation method thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106105783A (en) * 2016-08-01 2016-11-16 景洪宏臻农业科技有限公司 A kind of batch production Boletus aereus cultural method
CN106866205A (en) * 2017-01-24 2017-06-20 中国科学院昆明植物研究所 A kind of stropharia rugoso-annulata plantation matrix and preparation method thereof
CN106866210A (en) * 2017-03-09 2017-06-20 海门市利欣农产品有限公司 A kind of bolete culture base-material and preparation method thereof
CN109392593A (en) * 2018-12-03 2019-03-01 贵州家鑫食用菌有限公司 A method of beefsteak mushroom is planted using Pleurotus eryngii dead meal
CN112400610A (en) * 2020-11-27 2021-02-26 贵州百居关农旅开发有限公司 Culture medium for boletus and preparation method thereof

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