CN103880540A - Boletus edulis culturing base stock and preparation method thereof - Google Patents

Boletus edulis culturing base stock and preparation method thereof Download PDF

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CN103880540A
CN103880540A CN201410115176.1A CN201410115176A CN103880540A CN 103880540 A CN103880540 A CN 103880540A CN 201410115176 A CN201410115176 A CN 201410115176A CN 103880540 A CN103880540 A CN 103880540A
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peck
sing
parts
culture base
preparation
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葛静波
陈春化
王浩东
沈业庭
季林章
孙晓亮
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SIYANG COUNTY INSTITUTE OF AGRICULTURAL SCIENCES
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SIYANG COUNTY INSTITUTE OF AGRICULTURAL SCIENCES
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Abstract

The invention provides a boletus edulis culturing base stock and a preparation method thereof, and relates to the technical field of edible fungi cultivation. The culturing base stock is prepared from the following components: lentinus edodes slag, rice bran, chicken manure, cured hay, watermeion peel, sorghum straw, urea, plant ash, calcium superphosphate, forchlorfenuron, theophylline, calcium carbonate and quicklime. The preparation method of the culturing base stock comprises the following steps: (1) preparing materials; (2) crushing; (3) mixing; (4) fermenting; (5) packaging. The boletus edulis culturing base stock and the preparation method thereof are scientific and reasonable, the prepared culturing base stock can be used for fruiting the boletus edulis at an early date and prolonging the fruiting period, and the nutritional value of the boletus edulis can be improved.

Description

Culture base-material of a kind of Suillus albidipes (Peck) Sing. and preparation method thereof
Technical field
The present invention relates to the preparation method of culture base-material He this culture base-material in a kind of fungus growing technique field, particularly a kind of Suillus albidipes (Peck) Sing..
Background technology
Suillus albidipes (Peck) Sing., formal name used at school King Boletus, main another name has big foot mushroom, white ox head, Huang Qiaoba, stir-fry bacterium, also claims thigh mushroom, reticulate pattern bolete, belongs to Mycophyta.The sporophore of Suillus albidipes (Peck) Sing. is meat, and the flat semisphere of cap is smooth, not sticky, presents light naked look, and bacterial context white is plump, canopy brown, and diameter maximum can reach 25 centimetres, 1 kilopond, cap is thicker, has many apertures below, and the utmost point, like beef liver, hence obtains one's name.And Suillus albidipes (Peck) Sing. delicious flavour, food flavor is fragrant and sweet good to eat, nutritious, is a kind of worldwide famous edible mushrooms.In addition, Suillus albidipes (Peck) Sing. has sauce fragrance, can be used as medicine, and is famous and precious rare medicinal material.
The history of China's artificial culture Suillus albidipes (Peck) Sing. is comparatively of short duration, the complicacy of the field growth of Suillus albidipes (Peck) Sing. on the one hand, a lot of Suillus albidipes (Peck) Sing.s have symbiotic plant, with the symbiotic plant lower growth that influences each other, certain puzzlement that this causes to artificial culture, the sporophore that is on the other hand Suillus albidipes (Peck) Sing. is larger, cultivation needs larger space and the management of science, need to form the Cultivation management mode that is different from other edible mushrooms, this two aspect has caused the tame difficulty of Suillus albidipes (Peck) Sing., is also to cause one of reason that current Suillus albidipes (Peck) Sing. yields poorly.
Along with in recent years, people increase day by day for the demand of Suillus albidipes (Peck) Sing., overcome above-mentioned difficulties, and the Suillus albidipes (Peck) Sing. that cultivates high-yield high-quality amount becomes the key of artificial culture Suillus albidipes (Peck) Sing., and wherein, the suitable efficient culture base-material of How to choose becomes the most important thing.Existing artificial culture culture base-material, adopt the agricultural byproducts such as wood chip, rice bran is major ingredient more, seldom adopts discarded bacterium slag and other waste, causes on the one hand this increase, is to gather materials on the spot on the other hand, causes the waste of resource.In the case of the scale of Edible Fungi increases day by day at present, increase the utilization of waste, improve the recyclability of resource, for society and self-growth, all there is good economic benefit.
Summary of the invention
Goal of the invention: for above-mentioned deficiency, overcome the defect of prior art, the object of this invention is to provide a kind of preparation method of culture base-material He this culture base-material of Suillus albidipes (Peck) Sing..
A culture base-material for Suillus albidipes (Peck) Sing., is made up of the component of following mass fraction:
Lentinus Edodes fungus slag 120-200 part, rice bran 40-85 part, chicken manure 25-55 part, hay 32-70 part, Watermelon rind 40-90 part, broomcorn straw 45-85 part, urea 8-20 part, plant ash 22-45 part, calcium superphosphate 10-18 part, forchlorfenuron 0.5-2 part, theophylline 0.5-1.5 part, calcium carbonate 20-60 part and unslaked lime 45-85 part.
As preferably, hay is made through air-dry, and water content is between 10%-25%.
As preferably, the mass fraction of Watermelon rind is 40-75 part.
A preparation method for the culture base-material of Suillus albidipes (Peck) Sing., comprises the following steps:
(1) get the raw materials ready: Lentinus Edodes fungus slag 120-200 part, rice bran 40-85 part, chicken manure 25-55 part, hay 32-70 part, Watermelon rind 40-90 part, broomcorn straw 45-85 part, urea 8-20 part, plant ash 22-45 part, calcium superphosphate 10-18 part, forchlorfenuron 0.5-2 part, calcium carbonate 20-60 part and unslaked lime 45-85 part;
(2) pulverize: the above-mentioned hay taking, Watermelon rind and broomcorn straw are pulverized, and particle median size is between 0.8-1.5mm;
(3) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keep the water content of mixture between 55%-75%, pH value 5.1-6.0;
(4) fermentation: after stirring, be 15-25 ℃ in temperature, the environment bottom fermentation of relative air humidity 65%-75%;
(5) pack: after thing fermentation ends to be mixed, mixture is packed in bacterium bag, sealing, makes.
As preferably, the fermentation time of fermentation stage is 30-60 day.
As preferably, bacterium bag is acrylic plastering bacterium bag.
Beneficial effect: culture base-material of Suillus albidipes (Peck) Sing. provided by the invention and preparation method thereof, utilize Lentinus Edodes fungus slag, rice bran and hay for major ingredient, coordinate Watermelon rind, broomcorn straw and chicken manure, said components nutritionally makes up mutually, meets the nutritional needs of Suillus albidipes (Peck) Sing. growth, makes Suillus albidipes (Peck) Sing. have comprehensive nutritive value, the re-using to resource on the other hand, accomplish to gather materials on the spot, reduced the cost of culture base-material, increased benefit.Culture base-material of Suillus albidipes (Peck) Sing. provided by the invention and preparation method thereof, scientific and reasonable, the culture base-material of making not only makes Suillus albidipes (Peck) Sing. realize fruiting, prolongation fruiting phase early, and improves the nutritive value of Suillus albidipes (Peck) Sing. itself.
Embodiment
In order further to understand the present invention, below in conjunction with embodiment, the preferred embodiment of the invention is described, but should be appreciated that these are described is for further illustrating the features and advantages of the present invention, rather than limiting to the claimed invention.
Embodiment 1
A culture base-material for Suillus albidipes (Peck) Sing., is made up of the component of following mass fraction:
45 parts of 120 parts of Lentinus Edodes fungus slags, 40 parts, rice bran, 25 parts of chicken manures, 32 parts, hay, 40 parts of Watermelon rinds, 45 parts of broomcorn straws, 8 parts, urea, 22 parts of plant ash, 10 parts of calcium superphosphate, 0.5 part of forchlorfenuron, 0.5 part of theophylline, 20 parts, calcium carbonate and unslaked limes.Wherein hay is made through air-dry, and water content is 18%.
A preparation method for the culture base-material of Suillus albidipes (Peck) Sing., comprises the following steps:
(1) get the raw materials ready: 45 parts of 120 parts of Lentinus Edodes fungus slags, 40 parts, rice bran, 25 parts of chicken manures, 32 parts, hay, 40 parts of Watermelon rinds, 45 parts of broomcorn straws, 8 parts, urea, 22 parts of plant ash, 10 parts of calcium superphosphate, 0.5 part of forchlorfenuron, 0.5 part of theophylline, 20 parts, calcium carbonate and unslaked limes;
(2) pulverize: the above-mentioned hay taking, Watermelon rind and broomcorn straw are pulverized, and particle median size is between 1.2mm;
(3) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keeping the water content of mixture is 62%, pH value 5.6;
(4) fermentation: after stirring, be 15 ℃ in temperature, the environment bottom fermentation of relative air humidity 66%, fermentation time is 45;
(5) pack: after thing fermentation ends to be mixed, mixture is packed in acrylic plastering bacterium bag, sealing, makes.
Embodiment 2
A culture base-material for Suillus albidipes (Peck) Sing., is made up of the component of following mass fraction:
85 parts of 200 parts of Lentinus Edodes fungus slags, 85 parts, rice bran, 55 parts of chicken manures, 70 parts, hay, 75 parts of Watermelon rinds, 85 parts of broomcorn straws, 20 parts, urea, 45 parts of plant ash, 18 parts of calcium superphosphate, 2 parts of forchlorfenurons, 1.5 parts of theophylline, 60 parts, calcium carbonate and unslaked limes.Wherein hay is made through air-dry, and water content is between 18%.
A preparation method for the culture base-material of Suillus albidipes (Peck) Sing., comprises the following steps:
(1) get the raw materials ready: 85 parts of 200 parts of Lentinus Edodes fungus slags, 85 parts, rice bran, 55 parts of chicken manures, 70 parts, hay, 75 parts of Watermelon rinds, 85 parts of broomcorn straws, 20 parts, urea, 45 parts of plant ash, 18 parts of calcium superphosphate, 2 parts of forchlorfenurons, 1.5 parts of theophylline, 60 parts, calcium carbonate and unslaked limes;
(2) pulverize: the above-mentioned hay taking, Watermelon rind and broomcorn straw are pulverized, and particle median size is between 1.2mm;
(3) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keeping the water content of mixture is 64%, pH value 5.2;
(4) fermentation: after stirring, be 15 ℃ in temperature, the environment bottom fermentation of relative air humidity 66%, fermentation time is 50;
(5) pack: after thing fermentation ends to be mixed, mixture is packed in acrylic plastering bacterium bag, sealing, makes.
Embodiment 3
A culture base-material for Suillus albidipes (Peck) Sing., is made up of the component of following mass fraction:
78 parts of 155 parts of Lentinus Edodes fungus slags, 72 parts, rice bran, 45 parts of chicken manures, 65 parts, hay, 65 parts of Watermelon rinds, 70 parts of broomcorn straws, 12 parts, urea, 32 parts of plant ash, 15 parts of calcium superphosphate, 1 part of forchlorfenuron, 0.8 part of theophylline, 43 parts, calcium carbonate and unslaked limes.Wherein hay is made through air-dry, and water content is between 18%.
A preparation method for the culture base-material of Suillus albidipes (Peck) Sing., comprises the following steps:
(1) get the raw materials ready: 78 parts of 155 parts of Lentinus Edodes fungus slags, 72 parts, rice bran, 45 parts of chicken manures, 65 parts, hay, 65 parts of Watermelon rinds, 70 parts of broomcorn straws, 12 parts, urea, 32 parts of plant ash, 15 parts of calcium superphosphate, 1 part of forchlorfenuron, 0.8 part of theophylline, 43 parts, calcium carbonate and unslaked limes;
(2) pulverize: the above-mentioned hay taking, Watermelon rind and broomcorn straw are pulverized, and particle median size is between 1.2mm;
(3) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keeping the water content of mixture is 67%, pH value 5.7;
(4) fermentation: after stirring, be 15 ℃ in temperature, the environment bottom fermentation of relative air humidity 66%, fermentation time is 38;
(5) pack: after thing fermentation ends to be mixed, mixture is packed in acrylic plastering bacterium bag, sealing, makes.
Utilizing the culture base-material of Suillus albidipes (Peck) Sing. cultivation conventional in prior art is control group, prepares culture base-material take weed tree sawdust and Semen Maydis powder as major ingredient, is control group 1.Embodiment 1-3 and control group 1 are carried out to Suillus albidipes (Peck) Sing. experiment in cultivation.In test, indoor temperature, humidity, illumination, ventilation management work and earthing condition are identical, observation fruiting time, fruiting phase and output.The Suillus albidipes (Peck) Sing. afterwards embodiment group and control group being cultivated carries out Analysis of Nutritional test.
Test-results is as follows:
(1) comparative analysis of fruiting time, fruiting phase and output.
The culture base-material control group of embodiment group and prior art, during budding fruiting time, fruiting and output have certain difference.
The fruiting time average of buddingging of embodiment group is done sth. in advance 2.5 days; Stop the fruiting time upper, embodiment group is on average later than control group 3.5 days, and the whole fruiting phase on average extends 6 days; In output, unit surface embodiment group on average increases production 4.7% than control group.
(2) difference of the Suillus albidipes (Peck) Sing. main nutrient composition of different culture media material cultivation, result is as shown in table 1:
The main nutrient composition content (unit :/100g dry weight) of table 1 different culture media material cultivation Suillus albidipes (Peck) Sing.
Figure DEST_PATH_IMAGE002A
As seen from table, the carbohydrate of embodiment group, protein, ash content and riboflavin content are higher than control group, and wherein, protein content and riboflavin content, be significantly higher than control group; Content and the control group of other nutritive ingredients remain basically stable.
Therefore, the culture base-material that the culture base-material preparation method of Suillus albidipes (Peck) Sing. provided by the invention makes, can make compared to prior art Suillus albidipes (Peck) Sing. shift to an earlier date fruiting, extend the fruiting phase, and on nutritive value higher than existing culture base-material, not only improve output but also reduced cost, very practical.
To the above-mentioned explanation of the disclosed embodiments, make professional and technical personnel in the field can realize or use the present invention.To be apparent for those skilled in the art to the multiple modification of these embodiment, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.

Claims (6)

1. a culture base-material for Suillus albidipes (Peck) Sing., is characterized in that being made up of the component of following mass fraction:
Lentinus Edodes fungus slag 120-200 part, rice bran 40-85 part, chicken manure 25-55 part, hay 32-70 part, Watermelon rind 40-90 part, broomcorn straw 45-85 part, urea 8-20 part, plant ash 22-45 part, calcium superphosphate 10-18 part, forchlorfenuron 0.5-2 part, theophylline 0.5-1.5 part, calcium carbonate 20-60 part and unslaked lime 45-85 part.
2. the culture base-material of Suillus albidipes (Peck) Sing. according to claim 1, is characterized in that: hay is made through air-dry, and water content is between 10%-25%.
3. the culture base-material of Suillus albidipes (Peck) Sing. according to claim 1, is characterized in that: the mass fraction of Watermelon rind is 40-75 part.
4. a preparation method for the culture base-material of Suillus albidipes (Peck) Sing. claimed in claim 1, is characterized in that: comprise the following steps:
(1) get the raw materials ready: Lentinus Edodes fungus slag 120-200 part, rice bran 40-85 part, chicken manure 25-55 part, hay 32-70 part, Watermelon rind 40-90 part, broomcorn straw 45-85 part, urea 8-20 part, plant ash 22-45 part, calcium superphosphate 10-18 part, forchlorfenuron 0.5-2 part, calcium carbonate 20-60 part and unslaked lime 45-85 part;
(2) pulverize: the above-mentioned hay taking, Watermelon rind and broomcorn straw are pulverized, and particle median size is between 0.8-1.5mm;
(3) mix: said components is mixed, after mixing, add water, regulate water content and the pH value of mixture, keep the water content of mixture between 55%-75%, pH value 5.1-6.0;
(4) fermentation: after stirring, be 15-25 ℃ in temperature, the environment bottom fermentation of relative air humidity 65%-75%;
(5) pack: after thing fermentation ends to be mixed, mixture is packed in bacterium bag, sealing, makes.
5. the preparation method of the culture base-material of Suillus albidipes (Peck) Sing. according to claim 4, is characterized in that: the fermentation time of fermentation stage is 30-60 day.
6. the preparation method of the culture base-material of Suillus albidipes (Peck) Sing. according to claim 4, is characterized in that: bacterium bag is acrylic plastering bacterium bag.
CN201410115176.1A 2014-03-26 2014-03-26 Boletus edulis culturing base stock and preparation method thereof Pending CN103880540A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105110841A (en) * 2015-07-25 2015-12-02 西峡县食用菌科研中心 Culture base material culturing Morchellaconica by utilization of mushroom residues and preparation method therefor
CN106673733A (en) * 2016-12-15 2017-05-17 防城港市蓝瀚达科技有限公司 Fermentation production method of boletus edulis culture medium
CN106866210A (en) * 2017-03-09 2017-06-20 海门市利欣农产品有限公司 A kind of bolete culture base-material and preparation method thereof
CN112400610A (en) * 2020-11-27 2021-02-26 贵州百居关农旅开发有限公司 Culture medium for boletus and preparation method thereof
CN112772276A (en) * 2021-01-20 2021-05-11 云南省热带作物科学研究所 Method for directly cultivating saprophytic bacteria by using waste boletus fuscogilus fungus bags

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105110841A (en) * 2015-07-25 2015-12-02 西峡县食用菌科研中心 Culture base material culturing Morchellaconica by utilization of mushroom residues and preparation method therefor
CN106673733A (en) * 2016-12-15 2017-05-17 防城港市蓝瀚达科技有限公司 Fermentation production method of boletus edulis culture medium
CN106866210A (en) * 2017-03-09 2017-06-20 海门市利欣农产品有限公司 A kind of bolete culture base-material and preparation method thereof
CN112400610A (en) * 2020-11-27 2021-02-26 贵州百居关农旅开发有限公司 Culture medium for boletus and preparation method thereof
CN112772276A (en) * 2021-01-20 2021-05-11 云南省热带作物科学研究所 Method for directly cultivating saprophytic bacteria by using waste boletus fuscogilus fungus bags

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Application publication date: 20140625