CN108059517A - Boletus aereus culture medium and its preparation method and application - Google Patents
Boletus aereus culture medium and its preparation method and application Download PDFInfo
- Publication number
- CN108059517A CN108059517A CN201711219945.2A CN201711219945A CN108059517A CN 108059517 A CN108059517 A CN 108059517A CN 201711219945 A CN201711219945 A CN 201711219945A CN 108059517 A CN108059517 A CN 108059517A
- Authority
- CN
- China
- Prior art keywords
- parts
- boletus aereus
- culture medium
- stalk
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to Boletus aereus technical field of cultivation more particularly to a kind of Boletus aereus culture medium and its preparation method and application.Boletus aereus culture medium provided by the invention, including wood chip, stalk, red soil, grain, rice bran, land plaster, trace mineral supplement and yeast extract, and wood chip is wooden, the blue or green hilllock oak of Chinese chestnut, alder and one kind or at least two mixture in birch withered tree, the limitation that existing Boletus aereus culture sill is limited by growth cycle, region or cultivation purposes is breached, expands cultivated area, while raw material sources are wide, quantity is more, it is at low cost, and preparation method is simple, it is with short production cycle.Boletus aereus culture medium using the present invention can accelerate the mycelial growth rate of Boletus aereus, shorten the bacteria time, while also improve cultivating rate, averagely produce mushroom weight and mushroom quality, so as to greatly increase production capacity, production cost is reduced, there is significant economic benefit.
Description
Technical field
The present invention relates to Boletus aereus technical field of cultivation more particularly to a kind of Boletus aereus culture medium and preparation method thereof
And application.
Background technology
Boletus aereus belongs to one kind of bolete, and primary alias has big foot mushroom, fries bacterium, also referred to as thigh mushroom, reticulate pattern beef liver
Bacterium belongs to Mycophyta.Boletus aereus not only delicious flavour but also full of nutrition, must rich in 17 kinds of human bodies containing a large amount of protein
The necessary mineral element of the human bodies such as amino acid and abundant phosphorus, potassium, calcium, magnesium, iron and zinc is needed, there are the work(such as anti-cancer, cough-relieving, tonifying Qi
Effect is weight-reducing edible mushroom, while has the effect of preferable to diseases such as hypertension, high cholesterol and hyperlipidemias, deep to be liked by consumer
Love.
In recent years, as people are to green and healthy pursuit, Boletus aereus is praised highly be subject to more people, and demand increasingly increases
Greatly, wild Boletus aereus quantity is drastically reduced, and the black ox bolete of artificial cultivation has become research emphasis.Usually cultivation black cattle liver
The raw material of the culture medium of bacterium are using rubber tree, flame tree, grapefruit, mango and weedtree etc., but there are the following problems：(1)
Rubber tree, flame tree, grapefruit and mango belong to tropical tree species, are grown on the torrid zone and subtropical zone, distributional region are limited,
And grapefruit, mango Pterostyrax economic fruit, flame tree category greening-tree do not belong to forest material, by its growth cycle and cultivation
The limitation of purposes, can not be as the raw material of the culture medium of stable cultivation Boletus aereus；(2) weedtree, such as Chinese catalpa wood, cypress, camphor tree
The broad-leaved woods such as wood, poplar, paulownia wood, resource distribution relatively dissipate, and in the majority with mixed forest, and single Tree Species Resources are not concentrated, number
Amount is few, it is also difficult to the raw material of the culture medium as cultivation Boletus aereus.
In view of this, the present invention is proposed.
The content of the invention
It is an object of the invention to provide a kind of Boletus aereus culture mediums, breach existing Boletus aereus culture medium based raw material
The limitation limited by growth cycle, region or cultivation purposes, expands cultivated area, while raw material sources are wide, and quantity is more,
It is at low cost.
The second object of the present invention is to provide a kind of preparation method of Boletus aereus culture medium, and preparation method is simple, raw
The production cycle is short, rationally using resource, reduces the cost of culture base-material, adds economic benefit.
The third object of the present invention, should in the application in a kind of cultural method of above-mentioned culture medium in Boletus aereus is provided
Culture medium can accelerate the mycelial growth rate of Boletus aereus, shorten the bacteria time, while also improve cultivating rate, averagely produce mushroom
Weight and mushroom quality so as to greatly increase production capacity, reduce production cost, have significant economic benefit.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of Boletus aereus culture medium, is mainly made of the raw material of following parts by weight：250-500 parts of wood chip, stalk
250-500 parts, 100-200 parts of red soil, 100-200 parts of grain, 30-60 parts of rice bran, 1-2 parts of land plaster, trace mineral supplement
0.25-0.5 parts and 0.2-0.4 parts of yeast extract；
Wherein, the wood chip is wooden, the blue or green hilllock oak of Chinese chestnut, alder and one kind in birch withered tree or at least two mixing
Object.
Further, the Boletus aereus culture medium, is mainly made of the raw material of following parts by weight：Wood chip 300-400
It is part, 300-400 parts of stalk, 120-180 parts of red soil, 120-180 parts of grain, 40-50 parts of rice bran, 1.2-1.6 parts of land plaster, micro
0.24-0.32 parts of 0.3-0.4 parts of element additive and yeast extract.
Preferably, the Boletus aereus culture medium, is mainly made of the raw material of following parts by weight：350 parts of wood chip, stalk
350 parts, 140 parts of red soil, 140 parts of grain, 45 parts of rice bran, 1.4 parts of land plaster, 0.35 part of trace mineral supplement and yeast extract
0.3 part.
Further, the stalk be adlay stalk, cornstalk, rice stalk and one kind in wheat-straw or at least two it is mixed
Close object.
Further, the trace mineral supplement is mainly made of the raw material of following parts by weight：MgSO4 0.1-0.2
Part, KH2PO40.1-0.2 parts and MnSO40.005-0.015 parts；
Preferably, the trace mineral supplement is mainly made of the raw material of following parts by weight：MgSO40.12-0.17
Part, KH2PO40.12-0.17 parts and MnSO40.008-0.0012 parts.
It is furthermore preferred that the trace mineral supplement is mainly made of the raw material of following parts by weight：MgSO40.14 part,
KH2PO40.15 part and MnSO40.01 part.
Further, the biodiversity content of the Boletus aereus culture medium is 50-68%, is preferably 55-62%.
The preparation method of above-mentioned Boletus aereus culture medium, comprises the following steps：
(a) pretreatment of raw material：Wood chip and stalk are prewetted fermentation reactor system；
(b) treated wood chip and stalk and red soil, grain, rice bran and land plaster are uniformly mixed, form material heap；
(c) it after trace mineral supplement and yeast extract water dissolution, will be sprayed in the material heap of step (b) formation, obtain
To Boletus aereus culture medium.
Further, wood chip is prewetted fermentation reactor system 60-90 days in step (a), is preferably wooden per turning in 15-30 days once
Piece is prewetted fermentation reactor system 90 days, and every turning in 30 days is once.
Further, stalk is prewetted fermentation reactor system 3-5 days in step (a), is preferably 4 days.
Further, the good raw material of heap is first is prewetted by fermentation reactor system of prewetting in step (a) with water drenching, then in stockpile
Surface covers one layer of plastic foil and ferments.
Application of the above-mentioned Boletus aereus culture medium in the cultural method of Boletus aereus.
Compared with prior art, the beneficial effects of the present invention are：
1. Boletus aereus culture medium provided by the invention is using woody raw material and the herbaceous material cooperation of grass tree section, by black cattle liver
Bacterium becomes from wood destroying fungi for the rotten mushroom of half wood corruption, half grass, and wood chip is in wooden, the blue or green hilllock oak of Chinese chestnut, alder and birch withered tree
It is a kind of or at least two mixture, breach the cultivation culture raw material of existing Boletus aereus by growth cycle, region or cultivation
The limitation of the limitation of purposes, expands cultivated area；And woody raw material and herbaceous material source are wide, quantity is more.
2. Boletus aereus culture medium provided by the invention takes full advantage of the stalk of gramineae farm crop, realize become give up into
Treasured reduces the pollution be clear and burn agricultural crop straw to air, protects environment, reduce cost.
Nutritional need necessary to 3. Boletus aereus culture medium provided by the invention disclosure satisfy that Boletus aereus growth, accelerates
The mycelial growth rate of Boletus aereus shortens the bacteria time, while also improves cultivating rate, averagely produces mushroom weight and mushroom quality,
So as to greatly increase production capacity, production cost is reduced, there is significant economic benefit.
4. the preparation method of Boletus aereus culture medium provided by the invention is simple for process, with short production cycle, production is reduced
Cost is worth application.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is part of the embodiment of the present invention to apply example, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
In the cultivation field of Boletus aereus, such as rubber tree, flame tree, grapefruit, mango and weedtree work are usually selected
To cultivate the raw material of the culture medium of Boletus aereus, but these raw materials are limited by growth cycle, region or cultivation purposes, are had
Certain limitation.
The present invention provides a kind of Boletus aereus culture mediums, are mainly made of the raw material of following parts by weight：Wood chip 250-
500 parts, it is 250-500 parts of stalk, 100-200 parts of red soil, 100-200 parts of grain, 30-60 parts of rice bran, 1-2 parts of land plaster, micro
0.2-0.4 parts of 0.25-0.5 parts of element additive and yeast extract.
Boletus aereus culture medium provided by the invention is using woody raw material (such as Chinese chestnut wood, blue or green hilllock oak, alder, birch withered tree)
Coordinate with grass tree section herbaceous material (stalk), then add red soil, millet, rice bran, land plaster, trace mineral supplement and yeast extract
It is formulated, wherein, wood chip and stalk provide the compositions such as lignin, cellulose for compost；Grain and rice bran are mainly to provide
The nutritional ingredients such as carbohydrate；Red soil provides the nutritional ingredients such as iron aluminium element for culture medium；Land plaster is used to increase compost
Calcium content；Trace mineral supplement provides the trace elements such as magnesium, manganese, potassium, phosphorus, sulphur for culture medium；Yeast extract provides albumen
The nutriments such as matter and amino acid improve fermentation rate.
Yeast extract refers to cream yeast extract, rich in complete protein, balanced essential amino acid and the dimension life of B races
Element, nucleotide, trace element etc., are the primary raw materials in ideal biological culture medium raw material and fermentation industry, effect
It is suitable with 8 times of yeast, the throughput rate of strain and fermented product yield can be greatly improved.In a kind of preferred reality of the present invention
It applies in mode, yeast extract is selected from Shanghai Fu Ren trade Co., Ltds.
In the present invention, wood chip is wooden, the blue or green hilllock oak of Chinese chestnut, alder and one kind in birch withered tree or at least two mixing
Object.For example, above-mentioned wood chip is typical but not restricted is：Chinese chestnut wood, blue or green hilllock oak, alder, birch withered tree, Chinese chestnut Mu Heqing hilllocks oak
The combination of wood, the combination of Chinese chestnut wood and alder, the combination of Chinese chestnut wood and birch withered tree, the combination of blue or green hilllock oak and alder, blue or green hilllock oak
The combination of wood and birch withered tree, the combination of alder and birch withered tree, the combination of Chinese chestnut wood, blue or green hilllock oak and alder, Chinese chestnut wood, blue or green hilllock oak
The combination of wood and birch withered tree, the combination of Chinese chestnut wood, alder and birch withered tree, the combination of blue or green hilllock oak, alder and birch withered tree, Chinese chestnut
The combination of wooden, blue or green hilllock oak, alder and birch withered tree.
The present invention is become Boletus aereus for half wood from wood destroying fungi using woody raw material and the herbaceous material cooperation of grass tree section
The rotten mushroom of rotten half grass breaches cultivation culture raw material such as rubber tree, flame tree, shaddock wood and the mango wood of existing Boletus aereus
Deng the limitation limited by growth cycle, region or cultivation purposes, cultivated area is expanded.Meanwhile take full advantage of grass
The stalk of section crops, realization are turned waste into wealth, and are reduced the pollution be clear and burn agricultural crop straw to air, are protected environment, reduce
Cost.
Boletus aereus culture medium provided by the invention, is counted in parts by weight,
Wood chip is typical but nonrestrictive content is：200 parts, 220 parts, 250 parts, 280 parts, 300 parts, 320 parts, 350 parts,
380 parts, 400 parts, 420 parts, 450 parts, 480 parts or 500 parts；
Stalk is typical but nonrestrictive content is：200 parts, 220 parts, 250 parts, 280 parts, 300 parts, 320 parts, 350 parts,
380 parts, 400 parts, 420 parts, 450 parts, 480 parts or 500 parts；
Red soil is typical but nonrestrictive content is：100 parts, 110 parts, 120 parts, 130 parts, 140 parts, 150 parts, 160 parts,
170 parts, 180 parts, 190 parts or 200 parts；
Grain is typical but nonrestrictive content is：100 parts, 110 parts, 120 parts, 130 parts, 140 parts, 150 parts, 160 parts,
170 parts, 180 parts, 190 parts or 200 parts；
Rice bran is typical but nonrestrictive content is：30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38
Part, 39 parts, 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, 46 parts, 47 parts, 48 parts, 49 parts, 50 parts, 51 parts, 52 parts, 53
Part, 54 parts, 55 parts, 56 parts, 57 parts, 58 parts, 59 parts or 60 parts；
Land plaster is typical but nonrestrictive content is：1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts,
1.7 parts, 1.8 parts, 1.9 parts or 2 parts；
Trace mineral supplement is typical but nonrestrictive content is：0.25 part, 0.26 part, 0.27 part, 0.28 part, 0.29
Part, 0.3 part, 0.31 part, 0.32 part, 0.33 part, 0.34 part, 0.35 part, 0.36 part, 0.37 part, 0.38 part, 0.39 part, 0.4
Part, 0.41 part, 0.42 part, 0.43 part, 0.44 part, 0.45 part, 0.46 part, 0.47 part, 0.48 part, 0.49 part or 0.5 part；
Yeast extract is typical but nonrestrictive content is：0.2 part, 0.21 part, 0.22 part, 0.23 part, 0.24 part, 0.25
Part, 0.26 part, 0.27 part, 0.28 part, 0.29 part, 0.3 part, 0.31 part, 0.32 part, 0.33 part, 0.34 part, 0.35 part, 0.36
Part, 0.37 part, 0.38 part, 0.39 part or 0.4 part.
As the optional embodiment of the present invention, Boletus aereus culture medium is mainly made of the raw material of following parts by weight：
300-400 parts of wood chip, 300-400 parts of stalk, 120-180 parts of red soil, 120-180 parts of grain, 40-50 parts of rice bran, land plaster
0.24-0.32 parts of 1.2-1.6 parts, 0.3-0.4 parts of trace mineral supplement and yeast extract.
As the preferred embodiments of the invention, the Boletus aereus culture medium, mainly by the raw material of following parts by weight
It is made：350 parts of wood chip, 350 parts of stalk, 140 parts of red soil, 140 parts of grain, 45 parts of rice bran, 1.4 parts of land plaster, trace element add
Add 0.3 part of 0.35 part of agent and yeast extract.
In the preferred embodiment of the present invention, stalk is one in adlay stalk, cornstalk, rice stalk and wheat-straw
Kind or at least two mixture.For example, above-mentioned stalk is typical but not restricted is：Adlay stalk, cornstalk, rice stalk are small
Straw, the combination of adlay stalk and cornstalk, the combination of adlay stalk and rice stalk, the combination of adlay stalk and wheat-straw, cornstalk and
The combination of the combination of rice stalk, cornstalk and wheat-straw, the combination of rice stalk and wheat-straw, adlay stalk, cornstalk and rice stalk
Combination, the combination of adlay stalk, cornstalk and wheat-straw, the combination of adlay stalk, rice stalk and wheat-straw, cornstalk, rice stalk
With the combination of wheat-straw, the combination of adlay stalk, cornstalk, rice stalk and wheat-straw.
The trace mineral supplement is mainly made of the raw material of following parts by weight：MgSO40.1-0.2 parts, KH2PO4
0.1-0.2 parts and MnSO40.005-0.015 parts；
Trace mineral supplement in the Boletus aereus culture medium of the present invention, is counted in parts by weight,
MgSO4Typical but nonrestrictive content is：0.1 part, 0.11 part, 0.12 part, 0.13 part, 0.14 part, 0.15 part,
0.16 part, 0.17 part, 0.18 part, 0.19 part or 0.2 part；
KH2PO4Typical but nonrestrictive content is：0.1 part, 0.11 part, 0.12 part, 0.13 part, 0.14 part, 0.15 part,
0.16 part, 0.17 part, 0.18 part, 0.19 part or 0.2 part；
MnSO4Typical but nonrestrictive content is：0.005 part, 0.006 part, 0.007 part, 0.008 part, 0.009 part,
0.01 part, 0.011 part, 0.012 part, 0.013 part, 0.014 part or 0.015 part.
As a kind of optional embodiment of the present invention, the trace mineral supplement is mainly by the original of following parts by weight
Material composition：MgSO40.019-0.22 parts, KH2PO40.03-0.033 parts and MnSO40.001-0.004 parts.
As a kind of preferred embodiment of the present invention, the trace mineral supplement is mainly by the original of following parts by weight
Material composition：MgSO40.14 part, KH2PO40.15 part and MnSO40.01 part.
Boletus aereus culture medium provided by the invention is using woody raw material and the herbaceous material cooperation of grass tree section, by Boletus aereus
Become from wood destroying fungi for the rotten mushroom of rotten half grass of half wood, breach the material that Boletus aereus cultivation breaks through existing culture base-material,
The limitation limited by growth cycle, region or cultivation purposes, expands cultivated area；And woody raw material comes with herbaceous material
Source is wide, quantity is more.
As a kind of optional embodiment of the present invention, the biodiversity content of the Boletus aereus culture medium is 50-
68%, it is preferably 55-62%.
The adjusting of the biodiversity content of Boletus aereus culture medium is known to the skilled person common sense.
The preparation method of above-mentioned Boletus aereus culture medium, comprises the following steps：
(a) pretreatment of raw material：Wood chip and stalk are prewetted fermentation reactor system；
(b) treated wood chip and stalk and red soil, grain, rice bran and land plaster are uniformly mixed, form material heap；
(c) it after trace mineral supplement and yeast extract water dissolution, will be sprayed in the material heap of step (b) formation, obtain
To Boletus aereus culture medium.
In step (a), wood chip is prewetted fermentation reactor system 60-90 days, every turning in 30 days is once, it is therefore an objective to make wood chip fully soft
Change, is decomposed.In the present invention, wood chip prewet composting time typical case but nonrestrictive be：60 days, 65 days, 70 days, 75 days, 80 days,
85 days or 90 days.As a kind of preferred embodiment of the present invention, in step (a) specification of wood chip for (0.1-0.4) cm ×
(1.0-2.0) cm contributes to wood chip preferably to soften decomposed.
In step (a), stalk is prewetted composting 3-5 days, it is therefore an objective to stalk be allowed fully to soften, is decomposed.In the present invention, stalk
Prewet composting time typical case but nonrestrictive be：3 days, 4 days or 5 days.As a kind of preferred embodiment of the present invention, step
(a) specification of stalk is (0.1-0.2) cm × (1.0-2.0) cm in, stalk is contributed to preferably to soften decomposed.
As a kind of preferred embodiment of the present invention, fermentation reactor system is prewetted in step (a) first to use the good raw material of heap
Water drenching is prewetted, and then covering one layer of plastic foil on stockpile surface ferments.
As a kind of preferred embodiment of the present invention, in step (a) turning be by the upper layer material for expecting heap turn to lower floor, under
Layered material turns to upper strata, surface layer material turns to center, center material turns to surface layer, is uniformly mixed, and supplements lost moisture.The mesh of turning
Be to make the sufficient fermentation maturity of wood chip.
As a kind of preferred embodiment of the present invention, red soil needs to sieve before use, screens out diameter more than 0.5cm
Grain, so as to get culture medium it is more uniform.
As a kind of preferred embodiment of the present invention, grain filters out moisture after soaking 10-15h, so as to preferably
Form culture medium.
Application of the above-mentioned Boletus aereus culture medium in the cultural method of Boletus aereus.
Specifically, a kind of cultural method of Boletus aereus：
(1) bottle, sterilize：Culture medium is fitted into the plastic bottle of 1100mL-1800mL, in the interposition of matrix after bottling
Punching is put to bottom, plastic bottle shoulder to the high 4cm of bottleneck sterilizes 80-100 minutes at 120-125 DEG C, spare after cooling；
(2) inoculation, bacteria：Solid Boletus aereus strain is inoculated into the culture medium in step (a), inoculum concentration is every bottle
Blake bottle after inoculation is placed in 27-30 DEG C of temperature, CO by 40g2Concentration is 1500-2500ppm, the culturing room of humidity 65-75%
In, half-light culture covers with matrix in 30-40 days to mycelia can；
(3) earthing induction fruiting：The overburden layer of 4-5cm thickness is covered on the surface of bacterium bottle, then in 27-30 DEG C of temperature, CO2
Concentration 1500-2500ppm, humidity 90-98% subdued light conditions under cultivate, on the one hand cultivate mycelia regrowed in overburden layer,
On the other hand the edaphon growth of culture induction fruit-body formation, induction fruit body primordium is formed, fruiting, is cultivated to 8-10
Its mycelia covers with overburden layer, and the quantity of the edaphon of fruit-body formation is induced to increase considerably, into fruiting；
(4) fruiting, growth and harvesting
In 27-30 DEG C of temperature, CO2It is cultivated under conditions of concentration 1000-1500ppm, humidity 90-95%, illuminance 300LX
Fruiting in buddingging for the 4th day into fruiting, all sprouts, the 7th day starts to harvest, and harvesting in the 9th day finishes on the 5th day.
Embodiment 1
The Boletus aereus culture medium that embodiment 1 provides, is mainly made of the raw material of following parts by weight：Blue or green hilllock oak piece
250 parts, 250 parts of adlay stalk, 100 parts of red soil, 100 parts of grain, 30 parts of rice bran, 1 part of land plaster, MgSO40.1 part, KH2PO4
0.1 part, MnSO40.005 part and 0.2 part of yeast extract.
The preparation method for the Boletus aereus culture medium that embodiment 1 provides, includes the following steps：
(a) pretreatment of raw material：It is 0.4cm × 2.0cm by Chip treatment, is prewetted after building heap with water drenching, then in stockpile
Surface covers one layer of plastic foil and ferments, and ferments 60 days, every turning in 30 days is once；It is 0.2cm × 2.0m by stalk processing, builds
It is prewetted after heap with water drenching, then covering one layer of plastic foil on stockpile surface ferments, the composting 3 days of prewetting；Red soil sieves, sieve
Except diameter is more than 0.5cm particles；Grain filters out moisture after soaking 10h；
(b) treated wood chip, stalk, red soil and grain and rice bran and land plaster are uniformly mixed, form material heap；
(c) MgSO is weighed in parts by weight4、KH2PO4、MnSO4After yeast extract water dissolution, step (b) is sprayed at
In the material heap of formation, biodiversity content makes to obtain Boletus aereus culture medium biodiversity content for 55%.
Embodiment 2
The Boletus aereus culture medium that embodiment 2 provides, is mainly made of the raw material of following parts by weight：400 parts of alder piece,
400 parts of rice stalk, 180 parts of red soil, 180 parts of grain, 40 parts of rice bran, 1.2 parts of land plaster, 0.2 part of MgSO4, KH2PO4 0.1
0.32 part of part, 0.012 part of MnSO4 and yeast extract.
The preparation method for the Boletus aereus culture medium that embodiment 2 provides, includes the following steps：
(a) pretreatment of raw material：It is 0.2cm × 1.5cm by Chip treatment, is prewetted after building heap with water drenching, then in stockpile
Surface covers one layer of plastic foil and ferments, and ferments 90 days, every turning in 30 days is once；It is 0.1cm × 1.5cm by stalk processing,
It is prewetted after building heap with water drenching, then covering one layer of plastic foil on stockpile surface ferments, the composting 4 days of prewetting；Red soil sieves,
Diameter is screened out more than 0.5cm particles；Grain filters out moisture after soaking 12h；
(b) treated wood chip, stalk, red soil and grain and rice bran and land plaster are uniformly mixed, form material heap；
(c) MgSO is weighed in parts by weight4、KH2PO4、MnSO4After yeast extract water dissolution, step (b) is sprayed at
In the material heap of formation, biodiversity content makes to obtain Boletus aereus culture medium biodiversity content for 58%.
Embodiment 3
The Boletus aereus culture medium that embodiment 3 provides, is mainly made of the raw material of following parts by weight：Birch withered tree piece 500
Part, 500 parts of wheat-straw, 200 parts of red soil, 200 parts of grain, 60 parts of rice bran, 2 parts of land plaster, 0.15 part of MgSO4, KH2PO4
0.4 part of 0.15 part, 0.012 part of MnSO4 and yeast extract.
The preparation method for the Boletus aereus culture medium that embodiment 3 provides, includes the following steps：
(a) pretreatment of raw material：It is 0.1cm × 1.0cm by Chip treatment, is prewetted after building heap with water drenching, then in stockpile
Surface covers one layer of plastic foil and ferments, and ferments 90 days, every turning in 30 days is once；It is 0.1cm × 1.0cm by stalk processing,
It is prewetted after building heap with water drenching, then covering one layer of plastic foil on stockpile surface ferments, the composting 5 days of prewetting；Red soil sieves,
Diameter is screened out more than 0.5cm particles；Grain filters out moisture after soaking 15h；
(b) treated wood chip, stalk, red soil and grain and rice bran and land plaster are uniformly mixed, form material heap；
(c) MgSO is weighed in parts by weight4、KH2PO4、MnSO4After yeast extract water dissolution, step (b) is sprayed at
In the material heap of formation, biodiversity content makes to obtain Boletus aereus culture medium biodiversity content for 62%.
Embodiment 4
The Boletus aereus culture medium that embodiment 4 provides, is mainly made of the raw material of following parts by weight：Chinese chestnut wood chip 350
Part, 350 parts of cornstalk, 140 parts of red soil, 140 parts of grain, 45 parts of rice bran, 1.4 parts of land plaster, MgSO40.14 part, KH2PO4
0.15 part, MnSO40.01 part and 0.3 part of yeast extract.
According to the preparation method of the Boletus aereus culture medium described in embodiment 2, the Boletus aereus culture of embodiment 4 is made
Base.
Embodiment 5
The Boletus aereus culture medium that embodiment 5 provides, is mainly made of the raw material of following parts by weight：Chinese chestnut wood chip 200
Part, 200 parts of blue or green hilllock oak piece, 400 parts of cornstalk, 160 parts of red soil, 160 parts of grain, 48 parts of rice bran, 1.6 parts of land plaster, MgSO4
0.02 part, KH2PO40.15 part, MnSO40.012 part and 0.28 part of yeast extract.
According to the preparation method of the Boletus aereus culture medium described in embodiment 2, the Boletus aereus culture of embodiment 5 is made
Base.
Embodiment 6
The Boletus aereus culture medium that embodiment 6 provides, is mainly made of the raw material of following parts by weight：100 parts of alder piece,
300 parts of birch withered tree piece, 350 parts of cornstalk, 160 parts of red soil, 150 parts of grain, 42 parts of rice bran, 1.6 parts of land plaster, MgSO4 0.13
Part, KH2PO40.18 part, MnSO40.012 part and 0.32 part of yeast extract.
According to the preparation method of the Boletus aereus culture medium described in embodiment 2, the Boletus aereus culture of embodiment 6 is made
Base.
Comparative example 1
The Boletus aereus culture medium that comparative example 1 provides, is mainly made of the raw material of following parts by weight：Rubber wood timber wood chip
200 parts, 200 parts of adlay stalk, 80 parts of red soil, 80 parts of grain, 24 parts of rice bran, 0.8 part of land plaster, MgSO40.05 part, KH2PO4
0.08 part, MnSO40.001 part and 0.45 part of yeast extract.
According to the preparation method of the Boletus aereus culture medium described in embodiment 2, the Boletus aereus culture of comparative example 1 is made
Base.
Comparative example 2
The Boletus aereus culture medium that comparative example 2 provides, is mainly made of the raw material of following parts by weight：Blue or green hilllock oak piece
300 parts, 300 parts of adlay stalk, 120 parts of red soil, 120 parts of grain, 36 parts of rice bran, 1.2 parts of land plaster, MgSO40.08 part, KH2PO4
0.09 part, MnSO40.006 part and 0.1 part of yeast extract.
According to the preparation method of the Boletus aereus culture medium described in embodiment 2, the Boletus aereus culture of comparative example 2 is made
Base.
Comparative example 3
The Boletus aereus culture medium that comparative example 3 provides is with embodiment 4 the difference is that being not added with stalk.
Comparative example 4
The Boletus aereus culture medium that comparative example 4 provides is with embodiment 4 the difference is that being not added with land plaster.
Comparative example 5
The Boletus aereus culture medium that comparative example 5 provides is with embodiment 4 the difference is that being not added with MnSO4。
Comparative example 6
The Boletus aereus culture medium that comparative example 6 provides is with embodiment 4 the difference is that being not added with yeast extract.
Comparative example 7
The Boletus aereus culture medium that comparative example 7 provides is with embodiment 4 the difference is that wood chip is flame tree.
Comparative example 8
The Boletus aereus culture medium that comparative example 8 provides is with embodiment 4 the difference is that wood chip is mango wood.
Effect example
Be respectively adopted Boletus aereus culture medium that embodiment 1-6 and comparative example 1-8 are provided at identical conditions according to
The cultivation method of above-mentioned Boletus aereus.It is specifically, as follows：
(1) bottle, sterilize：Culture medium is fitted into the plastic bottle of 1500mL, is punched after bottling in the centre position of matrix
To bottom, plastic bottle shoulder to the high 4cm of bottleneck sterilizes 80 minutes at 121 DEG C, spare after cooling；
(2) inoculation, bacteria：Solid Boletus aereus strain is inoculated into the culture medium in step (a), inoculum concentration is every bottle
Blake bottle after inoculation is placed in 28 DEG C of temperature, CO by 40g2Concentration is 1500-2000ppm, in the culturing room of humidity 70%, secretly
Optical culture covers with matrix in 3 days to mycelia can；
(3) earthing induction fruiting：The overburden layer of 4cm thickness is covered on the surface of bacterium bottle, then in 28 DEG C of temperature, CO2Concentration
1500-2500ppm, humidity 95% subdued light conditions under cultivate, induction fruit body primordium formed, fruiting, cultivate long to 9 days mycelia
Full overburden layer, induces the quantity of the edaphon of fruit-body formation to increase considerably, into fruiting；
(4) fruiting, growth and harvesting
In 28 DEG C of temperature, CO2Concentration 1000-1500ppm, humidity 95% cultivate fruiting under conditions of illuminance 300LX,
In buddingging for the 4th day into fruiting, all sprout within the 5th day, the 7th day starts to harvest, and harvesting in the 9th day finishes.
The character of Boletus aereus is observed and recorded in the course of cultivation, first observes and counts the bacterium of each embodiment and comparative example
Kind sprout time, mycelial growth rate, mycelia cover with matrix situation；Harvested when Boletus aereus fructification is up to 75% maturity,
It weighs, observes and count fruiting situation, cultivating rate, every bottle of production fresh Boletus aereus weight and mushroom quality.Table 1 is using embodiment 1-
6 and comparative example 7 and 8 Boletus aereus culture medium observation statistical result, table 2 be using comparative example 1-6 Boletus aereus culture
The observation statistical result of base.
Table 1 uses the observation statistical result of embodiment 1-6 and the Boletus aereus culture medium of comparative example 7 and 8
Table 2 uses the observation statistical result of the Boletus aereus culture medium of comparative example 1-6
As can be seen from Table 1 and Table 2, the Boletus aereus culture medium provided using 1-6 of the embodiment of the present invention cultivates black ox
Liver bacterium, strain sprout time are 2-4 days, the strain sprout time (5-7 days) less than comparative example 1-6；Mycelial growth rate is
0.4-0.45cm/ days, the mycelial growth rate (0.32-0.36cm/ days) higher than comparative example 1-6；Mycelia covers with the time of matrix
For 31-35 days, the mycelia less than comparative example 1-6 covered with the time (38-44 days) of matrix；Fruiting is also more neat than comparative example 1-6；Go out
Mushroom rate be 95-99%, the cultivating rate (90-93%) higher than comparative example 1-6；The average single mushroom weight of every bottle of production is 101.3-108.6g,
The average single mushroom weight (89.5-94.5g) of every bottle of production higher than comparative example 1-6, while the quality of mushroom is better than comparative example 1-6.Thus
As it can be seen that Boletus aereus culture medium provided by the invention can accelerate the mycelial growth rate of Boletus aereus, shorten the bacteria time, together
When also improve cultivating rate, averagely produce mushroom weight and mushroom quality.
By embodiment 4 and comparative example 7, the comparison of comparative example 8 as can be seen that using Chinese chestnut wood, blue or green hilllock oak, alder and birch
Withered tree as wood chip source and use flame tree, mango carpentery workshop as wood chip source, put down by the speed of growth of Boletus aereus, cultivating rate
It is little to produce the quality differential of mushroom weight and mushroom, and Chinese chestnut wood, blue or green hilllock oak, alder and birch withered tree source are wide, it is at low cost,
From growth cycle, region or the limitation for cultivating purposes.
By the comparison of embodiment 4 and comparative example 1 and comparative example 2 as can be seen that the raw material of Boletus aereus culture medium forms
With compare Boletus aereus cultivation performance have a significant impact, Boletus aereus culture medium provided by the invention can advantageously promote black
The growth of bolete improves cultivating rate, the quality for averagely producing mushroom weight and mushroom.
By the comparison of embodiment 4 and comparative example 3 as can be seen that stalk has larger shadow to the cultivation performance of Boletus aereus
It rings, the present invention is coordinated using woody raw material and grass tree section herbaceous material (cornstalk, adlay stalk, rice stalk, wheat-straw), makes black ox
Liver bacterium culture medium can advantageously promote the growth of Boletus aereus, improve cultivating rate, the quality for averagely producing mushroom weight and mushroom.
Addition land plaster, MnSO can be seen that by the comparison of embodiment 4 and comparative example 4-64It can promote with yeast extract
Into the growth of Boletus aereus, cultivating rate, the quality for averagely producing mushroom weight and mushroom are improved.
In conclusion Boletus aereus culture medium provided by the invention can accelerate the mycelial growth rate of Boletus aereus, contracting
The short bacteria time, while also improve cultivating rate, averagely produce mushroom weight and mushroom quality, so as to greatly increase production capacity, reduce
Production cost has significant economic benefit, and breach the cultivation culture raw material of existing Boletus aereus by growth cycle,
The limitation of the limitation of domain or cultivation purposes, expands cultivated area, source is wide, quantity is more, is worthy of popularization.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe is described in detail the present invention with reference to foregoing embodiments, it will be understood by those of ordinary skill in the art that：Its according to
Can so modify to the technical solution recorded in foregoing embodiments either to which part or all technical characteristic into
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is not made to depart from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. a kind of Boletus aereus culture medium, which is characterized in that be mainly made of the raw material of following parts by weight：Wood chip 250-500
Part, 250-500 parts of stalk, 100-200 parts of red soil, 100-200 parts of grain, 30-60 parts of rice bran, 1-2 parts of land plaster, trace element
0.2-0.4 parts of 0.25-0.5 parts of additive and yeast extract；
Wherein, the wood chip is wooden, the blue or green hilllock oak of Chinese chestnut, alder and one kind or at least two mixture in birch withered tree.
2. Boletus aereus culture medium according to claim 1, which is characterized in that mainly by the raw material system of following parts by weight
Into：300-400 parts of wood chip, 300-400 parts of stalk, 120-180 parts of red soil, 120-180 parts of grain, 40-50 parts of rice bran, land plaster
0.24-0.32 parts of 1.2-1.6 parts, 0.3-0.4 parts of trace mineral supplement and yeast extract；
Preferably, the Boletus aereus culture medium, is mainly made of the raw material of following parts by weight：350 parts of wood chip, stalk 350
Part, 140 parts of red soil, 140 parts of grain, 45 parts of rice bran, 1.4 parts of land plaster, 0.35 part of trace mineral supplement and yeast extract 0.3
Part.
3. Boletus aereus culture medium according to claim 1, which is characterized in that the stalk is adlay stalk, cornstalk, water
One kind or at least two mixture in rice straw and wheat-straw.
4. Boletus aereus culture medium according to claim 1, which is characterized in that the trace mineral supplement is mainly by such as
The raw material composition of lower parts by weight：MgSO40.1-0.2 parts, KH2PO40.1-0.2 parts and MnSO40.005-0.015 parts；
Preferably, the trace mineral supplement is mainly made of the raw material of following parts by weight：MgSO40.12-0.17 parts,
KH2PO40.12-0.17 parts and MnSO40.008-0.0012 parts；
It is furthermore preferred that the trace mineral supplement is mainly made of the raw material of following parts by weight：MgSO40.14 part,
KH2PO40.15 part and MnSO40.01 part.
5. according to claim 1-4 any one of them Boletus aereus culture mediums, which is characterized in that the Boletus aereus culture medium
Biodiversity content for 50-68%, be preferably 55-62%.
6. such as the preparation method of claim 1-5 any one of them Boletus aereus culture mediums, which is characterized in that including following step
Suddenly：
(a) pretreatment of raw material：Wood chip and stalk are prewetted fermentation reactor system；
(b) treated wood chip and stalk and red soil, grain, rice bran and land plaster are uniformly mixed, form material heap；
(c) it after trace mineral supplement and yeast extract water dissolution, will be sprayed in the material heap of step (b) formation, obtain black
Beef liver bacterium culture medium.
7. preparation method according to claim 6, which is characterized in that wood chip is prewetted fermentation reactor system 60-90 in step (a)
My god, per turning in 15-30 days once, preferably wood chip is prewetted fermentation reactor system 90 days, and every turning in 30 days is once.
8. preparation method according to claim 6, which is characterized in that stalk is prewetted fermentation reactor system 3-5 days in step (a),
Preferably 4 days.
9. preparation method according to claim 6, which is characterized in that fermentation reactor system of prewetting in step (a) is first that heap is good
Raw material prewetted with water drenching, then stockpile surface cover one layer of plastic foil ferment.
10. such as application of the claim 1-5 any one of them Boletus aereus culture mediums in the cultural method of Boletus aereus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711219945.2A CN108059517A (en) | 2017-11-28 | 2017-11-28 | Boletus aereus culture medium and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711219945.2A CN108059517A (en) | 2017-11-28 | 2017-11-28 | Boletus aereus culture medium and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108059517A true CN108059517A (en) | 2018-05-22 |
Family
ID=62135924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711219945.2A Pending CN108059517A (en) | 2017-11-28 | 2017-11-28 | Boletus aereus culture medium and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108059517A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042063A (en) * | 2018-08-20 | 2018-12-21 | 周茂 | A kind of culture medium for cultivating, preparation method and a kind of Phlebopus portentosus batch production bacterium bag cultural method |
| CN109122051A (en) * | 2018-08-31 | 2019-01-04 | 景洪宏臻农业科技有限公司 | A kind of cultural method of Boletus aereus solid spawn |
| CN111296178A (en) * | 2020-03-26 | 2020-06-19 | 贵州福瑞斯新能源科技有限公司 | Method for producing oyster mushrooms by coix seed straws |
| CN114885749A (en) * | 2022-06-13 | 2022-08-12 | 贵州省山地资源研究所 | Bamboo fungus raw material cultivation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766137A (en) * | 2013-11-07 | 2014-05-07 | 石泉 | Phlebopus portentosus cultivating method |
| CN106673733A (en) * | 2016-12-15 | 2017-05-17 | 防城港市蓝瀚达科技有限公司 | Fermentation production method of boletus edulis culture medium |
| CN106866210A (en) * | 2017-03-09 | 2017-06-20 | 海门市利欣农产品有限公司 | A kind of bolete culture base-material and preparation method thereof |
| CN107318467A (en) * | 2017-08-23 | 2017-11-07 | 佛山推启农业研究院（普通合伙） | A kind of method of the blood red bolete of artificial cultivation |
-
2017
- 2017-11-28 CN CN201711219945.2A patent/CN108059517A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766137A (en) * | 2013-11-07 | 2014-05-07 | 石泉 | Phlebopus portentosus cultivating method |
| CN106673733A (en) * | 2016-12-15 | 2017-05-17 | 防城港市蓝瀚达科技有限公司 | Fermentation production method of boletus edulis culture medium |
| CN106866210A (en) * | 2017-03-09 | 2017-06-20 | 海门市利欣农产品有限公司 | A kind of bolete culture base-material and preparation method thereof |
| CN107318467A (en) * | 2017-08-23 | 2017-11-07 | 佛山推启农业研究院（普通合伙） | A kind of method of the blood red bolete of artificial cultivation |
Non-Patent Citations (1)
| Title |
|---|
| 马瑞霞等: "《普通高等教育"十三五"规划教材 食用菌栽培学》", 31 August 2017, 中国轻工业出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042063A (en) * | 2018-08-20 | 2018-12-21 | 周茂 | A kind of culture medium for cultivating, preparation method and a kind of Phlebopus portentosus batch production bacterium bag cultural method |
| CN109122051A (en) * | 2018-08-31 | 2019-01-04 | 景洪宏臻农业科技有限公司 | A kind of cultural method of Boletus aereus solid spawn |
| CN109122051B (en) * | 2018-08-31 | 2021-06-15 | 景洪宏臻农业科技有限公司 | Culture method of boletus aereus solid strain |
| CN111296178A (en) * | 2020-03-26 | 2020-06-19 | 贵州福瑞斯新能源科技有限公司 | Method for producing oyster mushrooms by coix seed straws |
| CN114885749A (en) * | 2022-06-13 | 2022-08-12 | 贵州省山地资源研究所 | Bamboo fungus raw material cultivation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chang et al. | The cultivation and environmental impact of mushrooms | |
| Oseni et al. | Effect of wheat bran supplement on growth and yield of oyster mushroom (Pleurotus ostreatus) on fermented pine sawdust substrate | |
| CN103652364B (en) | High-calcium leaf meal feed as well as production method and application thereof | |
| CN108059517A (en) | Boletus aereus culture medium and its preparation method and application | |
| CN106034742B (en) | A method of clitocybe maxima is produced using mulberry bar, bagasse and silkworm excrement | |
| CN106396807A (en) | Culture medium for improving bioconversion rate of edible fungi, preparation method of culture medium and cultivation method of edible fungi | |
| CN102498930A (en) | Method for cultivating straw mushrooms by utilizing pleurotus eryngii fungi residues | |
| Thakur | Present status and future prospects of tropical mushroom cultivation in India: a review | |
| CN106747820A (en) | A kind of method of culture medium of edible fungus, the preparation method of compost and culture edible mushroom | |
| CN102326472A (en) | Method for culturing lucid ganoderma by utilizing Chinese chestnut bud husk and waste branch | |
| Alemu | Cultivation of Pleurotus ostreatus on Grevillea robusta leaves at Dilla University, Ethiopia | |
| Thakur et al. | Advances in the cultivation technology of tropical mushrooms in India | |
| CN104106374B (en) | Utilize bagasse, mulberry bar and maize pulp to produce the method for Ji mushroom | |
| CN101601412A (en) | The purposes of bacillus subtilis and the production method of this bacterial strain | |
| CN109526551B (en) | Cultivation method of natural selenium-rich agaricus bisporus | |
| KR101518442B1 (en) | Method for producing organic fertilizer using cultural filtrate of wild ginseng and mushroom spent medium | |
| CN106467430A (en) | A kind of culture medium of agaricus bisporus mushroom slag for cultivating Volvariella volvacea (Bull.Ex Franch.) Singer． and its preparation method and application | |
| Mata et al. | Biotechnology for edible mushroom culture: a tool for sustainable development in Mexico | |
| Shehzad et al. | Performance of oyster mushroom (Pleurotus ostreatus) on cotton waste amended with maize and banana leaves | |
| CN104119942A (en) | Method for preparing phytic acid for water-soluble fertilizer as well as phytic acid and water-soluble fertilizer prepared by virtue of method | |
| CN107986845A (en) | Culture base-material using white fungus section waste log mushroom culture and preparation method thereof | |
| CN106688839A (en) | Plumeria rubra culture medium | |
| Khan et al. | Impact of various sterlization methods using different substrates for yield improvement of Pleurotus spp. | |
| Chang et al. | Status and prospect of mushroom industry in the Philippines | |
| CN106396964A (en) | Cultivation substrate for pleurotus eryngii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |