KR101518442B1 - Method for producing organic fertilizer using cultural filtrate of wild ginseng and mushroom spent medium - Google Patents
Method for producing organic fertilizer using cultural filtrate of wild ginseng and mushroom spent medium Download PDFInfo
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- KR101518442B1 KR101518442B1 KR1020140167235A KR20140167235A KR101518442B1 KR 101518442 B1 KR101518442 B1 KR 101518442B1 KR 1020140167235 A KR1020140167235 A KR 1020140167235A KR 20140167235 A KR20140167235 A KR 20140167235A KR 101518442 B1 KR101518442 B1 KR 101518442B1
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- organic fertilizer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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Abstract
The present invention relates to a method for producing an organic fertilizer using a plant tissue culture by-product which is fermented by adding a culture solution of a wild ginseng cultured muscle to a mushroom waste culture medium and a complex strain, and an organic fertilizer using the plant tissue culture by-product produced by the above- , The organic fertilizer using the wild ginseng culture filtrate and the mushroom culture by-product of the present invention replaces chemical pesticides and fertilizers and is used for cultivation of crops, it is possible not only to produce sufficient good agricultural products by supplying sufficient nutrients to the crops, It is possible to obtain an improvement effect and to protect against the diseases and insects of the crops, thereby preventing environmental pollution and preventing destruction of the ecosystem, which can be widely used in environmentally friendly organic farming.
Description
More particularly, the present invention relates to a method for producing organic fertilizer using a cultured wild ginseng culture solution and a mushroom waste medium, and more particularly, to a method for producing an organic fertilizer using a plant tissue culture by- And an organic fertilizer using the plant tissue culture by-products produced by the method.
Generally, the term organic fertilizer refers to a relative concept with inorganic fertilizer (chemical fertilizer), and generally includes an organic fertilizer containing an organic substance as its main raw material and a by-product fertilizer containing a by-product as a main raw material. Ash residue, humus, amino acid fermented by-product fertilizer, composted rice husk, sawdust or soil microorganism preparation.
The soil of our country is affected by fertilizer-oriented fertilization over a long period, excessive use of pesticides and chemical herbicides, and sequencing of one crop, resulting in degradation of crops, deterioration of quality, , Contamination of crops, decrease in yield, and decrease in productivity. Contrary to this problem of chemical fertilizer, high quality organic fertilizer promotes the growth of microorganisms in the soil, supplying various essential nutrients, enhancing bibbing power and water holding power, stabilizing crop growth and restoring soil ecosystem.
As the number of mushroom cultivated farmers increases, the amount of mushroom waste media is increasing with the change of production mode. The mushroom culture medium is composed of sawdust, sugar beet hail, cotton pomace, etc. and is suitable for producing organic fertilizer. The process is the process of softening the constituents of the mushroom culture medium, and thus it can be utilized as an organic fertilizer which is useful for growing and improving soil fertility.
The cultured ginseng roots cultured in the cultured ginseng adventitious roots (Ginseng roots) were separated from the ginseng adventitious roots, and then cultivated in the culture medium. Therefore, it is possible to utilize the fertilizer as a fertilizer for growing the crop.
Korean Patent No. 0856555 discloses a functional organic fertilizer and a method for producing the same, and Korean Patent Publication No. 2004-0026466 discloses a method for producing a natural organic compound fertilizer. However, And a method for producing an organic fertilizer using the mushroom waste medium have never been disclosed.
SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a fermentation by-product of mushrooms and ginseng cultivation roots rich in organic and inorganic minerals useful for improving crop growth and soil resistance, The present invention has been made to provide an organic fertilizer which is excellent in the growth of the finished crop and the effect of enhancing the harvest.
In order to solve the above problems,
(a) pulverizing the mushroom waste medium after cultivation of the mushroom;
(b) adding to the pulverized mushroom waste medium of step (a) the cultured solution of the cultured ginseng root and the complex strain; And
and (c) fermenting the mixed mixture of step (b). The present invention also provides a method for producing an organic fertilizer using the plant tissue culture by-product.
In addition, the present invention provides an organic fertilizer using the plant tissue culture by-products produced by the above production method.
The organic fertilizer using the wild ginseng culture filtrate and the mushroom culture by-product of the present invention replaces chemical pesticides and fertilizers and is used for cultivation of crops. Thus, sufficient nutrients can be supplied to the crops to produce good agricultural products, It is possible to prevent environmental pollution and destruction of ecosystem by protecting from the disease and insect pests of crops, and thus it can be widely used in environmentally friendly organic farming.
In order to achieve the object of the present invention,
(a) pulverizing the mushroom waste medium after cultivation of the mushroom;
(b) adding to the pulverized mushroom waste medium of step (a) the cultured solution of the cultured ginseng root and the complex strain; And
and (c) fermenting the mixed mixture of step (b). The present invention also provides a method for producing an organic fertilizer using the plant tissue culture by-product.
In the method of manufacturing an organic fertilizer using the plant tissue culture by-product according to an embodiment of the present invention, the mushroom waste medium of step (a) may include sawdust: sugar beet hail: cottonseed flour at 4-6: 2 to 4: 3, the mushroom may be produced for 40 to 60 days, and the discarded product after harvesting may be a by-product. Preferably, sawdust, sugar beet hail, cottonseed rape is mixed at a weight ratio of 5: 3: 2 After discarding the mushroom after 50 days of production and harvesting it may be a by-product, but is not limited thereto.
In the method for preparing an organic fertilizer using the plant tissue culture by-product according to an embodiment of the present invention, the culture filtrate of the wild ginseng cultured muscle is grown in a modified Murashige and Skoog medium supplemented with carbon source for 50 to 70 days, , And more specifically, may be a culture filtrate obtained by culturing a ginseng culturing muscle for 60 days, but the present invention is not limited thereto. Examples of the carbon source include, but are not limited to, sucrose and chitooligosaccharide.
In the production method of the organic fertilizer with the plant tissue culture by-products in accordance with one embodiment of the present invention, the "m-MS medium (modified Murashige and Skoog medium)" is KNO 3 1ℓ by suspension cultures of 2290 to 2294 mg, MgSO 4揃 7H 2 O 365 to 369 mg, NH 4 H 2 PO 4 273 to 277 mg, FeSO 4揃 7H 2 O 12 to 16 mg, Na 2揃 EDTA 17 to 20 mg, CaCl 2揃 2H 2 O 181 - Inositol 90-94 mg, Niconic acid 4-6 mg, Thiamine-HCl 4-6 mg, Pyridoxine-HCl 0.4-0.5 mg, , H 3 BO 3 3 ~ 6mg , MnSO 4 · H 2 O 8 ~ 10mg, KI 0.5 ~ 1.5mg, ZnSO 4 · 7H 2 O 0.5 ~ 1.5mg, CuSO 4 · 5H 2 O 0.1 ~ 0.3mg, Na 2 MoO 4 · 2H 2 O, and preferably from 0.05 to 0.15 mg of KNO 3 2291.67mg, MgSO 4 · 7H 2 O 366.67mg, NH 4 H 2 PO 4 275.00mg, FeSO 4 · 7H 2 O 13.75mg, Na 2 · EDTA 18.33mg, CaCl 2 · 2H 2 O 183.33mg, myo-inositol 91.67 mg, Nikon acid 4.58mg, 4.58mg -HCl thiamine, pyridoxine -HCl 0.46mg, H 3 BO 3 4.58mg , MnSO 4 · H 2 O 9.17mg, KI 0.92mg, ZnSO 4 · 7H 2 O 0.92mg, CuSO 4 0.19 mg of 5H 2 O, 0.10 mg of Na 2 MoO 4 .2H 2 O, but is not limited thereto.
In the method for producing an organic fertilizer using plant tissue culture by-products according to an embodiment of the present invention, the complex strain may be selected from the group consisting of Lactobacillus sp., Saccharomyces sp. cerevisiae and Bacillus subtilis ), but the present invention is not limited thereto.
The amount of the combined microorganism can be added within the range of the added amount of the microorganism added during the production of the conventional organic fertilizer. However, even if the by-product is fermented by using a large amount of microorganisms, It is not always necessary to limit the addition amount thereof because the effect of the pesticide can be obtained.
In the method for producing an organic fertilizer using plant tissue culture by-products according to an embodiment of the present invention, the mushroom waste medium pulverized product, the culture filtrate of cultured ginseng cultured muscle and the complex strain have a weight ratio of 450 to 550: 8 to 12: 0.5 to 1.5 , Preferably in a weight ratio of 500: 10: 1, but is not limited thereto.
In the method for producing an organic fertilizer using the plant tissue culture by-product according to an embodiment of the present invention, the fermentation is performed by putting a mixture of the mushroom pulverized medium pulverized product, the culture filtrate of the ginseng cultivation muscle and the complex strain in a plastic bag, , Fermenting at 25 to 30 ° C for 5 to 10 days, more preferably fermentation at 28 ° C for 7 days, but is not limited thereto.
The mushroom waste medium pulverized product, the culture solution of cultured ginseng cultured muscle, and the complex strain are mixed well and stirred sufficiently to maintain a uniform fermentation degree, and fermentation is preferably carried out with frequent stirring during the fermentation period. As described above, since the organic fertilizer produced by the pulverized product of the mushroom pulverized medium and the culture filtrate of the wild ginseng cultured muscle contains the microorganism, it shows the function of the pesticide together with the function of the fertilizer. In other words, if organic fertilizer is used for cultivation of crops due to microbial fermentation, sufficient nutrients can be supplied and the effect of improving land can be obtained. In particular, antimicrobials can protect crops from diseases and germs So that it is more effective for the soil in which the crop damage is caused. Therefore, when the organic fertilizer using the plant tissue culture by-product of the present invention, which has been fermented with inorganic and microorganisms in the culture medium of the mushroom waste culture medium and the cultured ginseng cultured muscle, is used, It can protect crops, increase yields, and grow high quality crops for consumers. In particular, since it is possible to protect the plant from diseases or insects without using chemical pesticides, environmental pollution can be prevented and ecosystem can be prevented from being destroyed.
The method for producing the organic fertilizer using the plant tissue culture by-product of the present invention is specifically
(a) Sawdust: Sugar beetle: Cotton husks are mixed at a weight ratio of 4: 6: 2 to 4: 1 to 3, and then the mushroom is produced for 40 to 60 days, and the discarded mushroom waste medium after harvesting is crushed step;
(b) culturing the pulverized mushroom waste medium of step (a) in a culture solution of cultured wild ginseng cultured muscle, and culturing the lactic acid bacteria ( Lactobacillus sp), yeast ( Saccharomyces sp. cerevisiae) and Bacillus subtilis (mixing into the composite strains consisting of Bacillus subtilis); And
(c) fermenting the mixed mixture of step (b) at 25 to 30 DEG C for 5 to 10 days,
More specifically,
(a) sawdust: sugar beet hail: cottonseed mixture at a weight ratio of 5: 3: 2 to produce a mushroom for 50 days, and then pulverizing the discarded mushroom waste medium after harvesting;
(b) culturing the pulverized mushroom waste medium of step (a) in a culture solution of cultured wild ginseng cultured muscle, and culturing the lactic acid bacteria ( Lactobacillus sp), yeast ( Saccharomyces sp. cerevisiae) and Bacillus subtilis (mixing into the composite strains consisting of Bacillus subtilis); And
(c) fermenting the mixed mixture of step (b) for 7 days at 28 ° C.
In addition, the present invention provides an organic fertilizer using the plant tissue culture by-products produced by the above production method. The organic fertilizer can be used according to a method commonly used in the art.
Hereinafter, the present invention will be described in detail with reference to Production Examples and Examples. However, the following Preparation Examples and Examples are illustrative of the present invention, and the content of the present invention is not limited to the following Production Examples and Examples.
Manufacturing example 1. Manufacture of organic fertilizer using plant tissue culture by-products
A method for producing an organic fertilizer using plant tissue culture by-products having an effect of supplying sufficient nutrients to crops as well as improving the land and protecting crops from diseases and insects is described below.
The mushroom waste medium used as the organic feed source of the organic fertilizer of the present invention was prepared by mixing sawdust: sugar beet hail: cottonseed rape at a weight ratio of 5: 3: 2, then producing oyster mushroom for 50 days, Were used. After the cultivation, the oyster mushroom was harvested and the remaining by-products were collected using a deaeration machine, and then put in a mixer and mixed for about 2 hours to mix the coarse grains and to mix the mushroom remaining after harvesting evenly.
The culture solution of the ginseng cultured muscle used as the mineral source of the organic fertilizer of the present invention was cultured in a modified Murashige and Skoog medium for 60 days after harvesting the cultured ginseng cultured muscle. The composition of m-MS medium (modified Murashige and Skoog medium) used for culture of wild ginseng cultured muscle was as shown in Table 1, and 2 kg of 1,200 ℓ of carbon source and 1 kg of chitooligosaccharide were added thereto for culturing.
The mushroom waste medium was mixed with the culture solution of the wild ginseng culture so that the filtrate was 2%. The mixture contains 30-40% of water and is suitable for microbial fermentation. Therefore, lactic acid bacteria ( Lactobacillus sp), yeast ( Saccharomyces sp. cerevisiae and Bacillus subtilis ) were added, and the mixture was thoroughly mixed for 3 hours, packed in a bag of 500 kg, and then completely sealed and fermented for 7 days in a 28 ° C incubator. The organic fertilizer using the plant tissue culture byproduct of the present invention was completed through a fermentation process that can prevent the damage of the crop by post-fermentation.
Example 1. Fertility effects of organic fertilizer using plant tissue culture by-products of the present invention
In order to investigate the fertilizing effect of the organic fertilizer cultivation by using the plant tissue culture byproducts prepared by the method of Preparation Example 1, the effects of fertilizing were examined in the field of garlic, pear, peach and ginseng.
① Gargle effect of garlic
In order to confirm the effect of garlic clustering, organic fertilizer was sprayed in autumn before planting garlic, and garlic was planted after rotary work, confirming the smelling effect of organic fertilizer on garlic cultivation. The fertilization of organic fertilizer was carried out by mixing 400kg / 10a of mixed organic fertilizer (40% of rice bran oil, 40% of brown sugar, 10% of bone meal, 10% of fish meal) and 340kg / 10a of mixed organic fertilizer And 60 kg / 10a of the organic fertilizer using the plant tissue culture by-products of the present invention.
It was confirmed that the treated garlic mixed with organic fertilizer using the plant tissue culture byproduct of the present invention showed no growth of insect pests in the harvested garlic the next year, and the growth of garlic was improved and the growth effect of 23% of the total production was shown. In addition, garlic is small, garlic less than 6 pages has a lower value of commodity, and the yield of garlic on top is increased by 25%. As a result, the growth of garlic was promoted without the occurrence of insect pests and the possibility of utilization of garlic which can be grown in garlic having a high commercial value was confirmed.
② fertilization effect in fruit crops
In order to confirm the fertilization effect in the fruit crops, organic fertilizer was applied around the circumference of the pear tree and the peach tree about 20 cm in the late autumn after harvesting, and the effect was confirmed. The organic fertilizer samples were prepared by mixing 10 kg / week of the mixed organic fertilizer (40% of rice bran oil, 40% of brown sugar, 10% of bone meal, 10% of fish meal) and 5 kg / week of mixed organic fertilizer The organic fertilizer using the plant tissue culture by-product of the present invention was divided into treatment groups treated with a mixture of 5 kg / week. There was no significant difference from the conventional organic fertilizer during the following year when the leaves were fruiting. However, when the fruit was matured and ripened, there were many differences. In the case of the peach fertilized with the organic fertilizer of the present invention, the yield of the top product with excellent shape and color was increased by 19%, the yield of the peach of 270 g or more was increased to 11%, and the sugar content was increased by 3 brix (Table 3).
(Color, shape, etc.)
(More than 270g)
In the case of the fertilizer applied with the organic fertilizer of the present invention, the yield of the top product having excellent shape and color was increased by 18%, the yield of the product of 600 g or more was increased to 21%, and the sugar content was increased by 2.5 brix (Table 4).
The fruit quality of the fruit of Korea is large, the sugar color is high, the color and shape are excellent, and it can be evaluated as the best quality. By using the organic fertilizer of the present invention, it is possible to produce fruit with improved sugar content and size, and thus it is expected to raise income of 20% or more of the farm household.
(Color, shape, etc.)
(600g or more)
③ Effect of reducing disorder of ginseng field
On the other hand, the most problematic problem in cultivation of ginseng is root rot disease and chaotic disorder. As a result, ginseng cultivation can be done only after 5 to 10 years of resting or other crop cultivation. This results in a significant loss of income along with a decrease in ginseng plantation area.
In order to investigate the effect of alleviating the chaotic disorder of the ginseng field, it was investigated whether or not the organic fertilizer of the present invention was sprinkled on the field where the ginseng was harvested, and the crop damage such as root rot caused by planting 1 year old ginseng Were observed for two years. The organic fertilizer samples were prepared by mixing 80 kg / 10a of the mixed organic fertilizer (40% rice bran oil, 40% brown rice husk, 10% bone meal and 10% fish meal), and organic fertilizer using the plant tissue culture by- Were divided into treatment groups treated with 80 kg / 10a. As a result, it was observed that the treated plants treated with the organic fertilizer of the present invention normally grow ginseng without any damage even for the second year. On the other hand, in the untreated pouch packed with the mixed organic fertilizer which is conventionally used without treating the organic fertilizer of the present invention, the damage of 1-year-old ginseng was so severe that it was almost impossible to grow. Therefore, it was confirmed that the fertilizer of the organic fertilizer of the present invention is superior in improving the root rot disease and the chaotic disorder which are the most problematic in ginseng cultivation.
Claims (8)
(b) contacting the pulverized mushroom waste medium of step (a) with a culture solution of wild ginseng cultured muscle and a complex strain comprising Lactobacillus sp., Saccharomyces cerevisiae and Bacillus subtilis in an amount of 450-550 : 8-12: Mixing in a weight ratio of 0.5 to 1.5; And
(c) fermenting the mixed mixture of step (b) at 25 to 30 ° C for 5 to 10 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108383584A (en) * | 2018-05-21 | 2018-08-10 | 合肥云都棉花有限公司 | A kind of organic fertilizer and preparation method thereof that output of cotton can be improved |
| KR102479161B1 (en) | 2022-07-11 | 2022-12-19 | 임성연 | Method of preparation for eco-friendly fertilizers using mushroom waste media |
| KR102605534B1 (en) * | 2022-08-03 | 2023-11-24 | 박헌의 | Snail feed composition with wild ginseng root culture media |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08181A (en) * | 1994-06-17 | 1996-01-09 | Noboru Yamamoto | Production of feed, fertilizer, etc., using waste mushroom cultivation medium |
| KR20060110891A (en) * | 2005-04-20 | 2006-10-26 | 박만조 | The manufacturing method of compound germanium organic fertilizer |
| KR20070090472A (en) * | 2006-03-03 | 2007-09-06 | 주식회사 진산삼바이오 | The artificial mass-productions of oyster mushroom using roots of tissue-cultured mountain ginseng |
| KR101224844B1 (en) * | 2010-12-08 | 2013-01-22 | 한경철 | Fertilizer containing germanum and method for producing a farm products using the same |
-
2014
- 2014-11-27 KR KR1020140167235A patent/KR101518442B1/en active IP Right Grant
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08181A (en) * | 1994-06-17 | 1996-01-09 | Noboru Yamamoto | Production of feed, fertilizer, etc., using waste mushroom cultivation medium |
| KR20060110891A (en) * | 2005-04-20 | 2006-10-26 | 박만조 | The manufacturing method of compound germanium organic fertilizer |
| KR20070090472A (en) * | 2006-03-03 | 2007-09-06 | 주식회사 진산삼바이오 | The artificial mass-productions of oyster mushroom using roots of tissue-cultured mountain ginseng |
| KR101224844B1 (en) * | 2010-12-08 | 2013-01-22 | 한경철 | Fertilizer containing germanum and method for producing a farm products using the same |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108383584A (en) * | 2018-05-21 | 2018-08-10 | 合肥云都棉花有限公司 | A kind of organic fertilizer and preparation method thereof that output of cotton can be improved |
| KR102479161B1 (en) | 2022-07-11 | 2022-12-19 | 임성연 | Method of preparation for eco-friendly fertilizers using mushroom waste media |
| KR102605534B1 (en) * | 2022-08-03 | 2023-11-24 | 박헌의 | Snail feed composition with wild ginseng root culture media |
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