KR102605534B1 - Snail feed composition with wild ginseng root culture media - Google Patents

Abstract

The present invention relates to a feed composition for edible snails using a culture medium for wild ginseng cultured roots, and more specifically, comprising a culture medium for wild ginseng cultured roots, rice bran, soybean powder, corn powder, and calcium carbonate. According to the present invention, it is possible to provide a feed manufacturing method using wild ginseng root culture medium, and to provide edible snails in which saponin is detected with feed manufactured by this method, and further increase the survival rate of snails, and chondroitin contained in snails. By increasing the sulfuric acid content, the added value of snails can be increased, which has the advantage of contributing to the improvement of farm income.

Description

Feed composition for edible snails using wild ginseng root culture medium {SNAIL FEED COMPOSITION WITH WILD GINSENG ROOT CULTURE MEDIA}

The present invention relates to a feed composition for edible snails, and more specifically, to a feed composition for edible snails using wild ginseng culture medium, which increases the survival rate of snails and the content of chondroitin sulfate by composing the feed composition using wild ginseng culture medium. It's about.

In general, edible snails contain a sticky mucus called mucin, the main component of which contains a large amount of chondroitin sulfate, a component similar to human sex hormones.

The chondroitin sulfate purifies the blood, prevents skin aging, and is known to be more effective than any animal or plant because its composition is similar to that of human sex hormones. In addition, it is effective in rejuvenating skin beauty, is rich in calcium, strengthens the kidneys, purifies the blood, has special effects on anemia and sinusitis, and is widely known as a stamina food for men.

Meanwhile, when cultivating edible snails, they are raised by mixing rice bran, calcium powder, corn flour, mulberry leaves, etc. as general feed, and the raised edible snails are used in various dishes and health supplements. It is used as a raw material for soap and cosmetics.

However, these edible snails have a poor initial survival rate, and it is difficult to create high added value, so the income is not very high compared to the labor invested, so the number of snail farmers is gradually decreasing.

KR 10-0393255 B1 KR 10-1272093 B1

Therefore, the purpose of the present invention is to raise edible snails using a feed composition using wild ginseng culture medium, thereby improving the initial survival rate by increasing the immunity of the snails and increasing the content of chondroitin sulfate contained in the snails, thereby adding added value to the snails. The aim is to provide a feed composition for edible snails using wild ginseng culture medium that can increase .

The feed composition for edible snails using the wild ginseng culture medium of the present invention to achieve the above object is characterized by containing wild ginseng culture medium, rice bran, soybean powder, corn powder, and calcium carbonate.

The wild ginseng culture medium is characterized in that it is a culture filtrate after culturing wild ginseng culture roots for 50 to 70 days with m-MS medium (modified Murashige and Skoog medium) to which a carbon source is added.

It is characterized in that it further contains Jeju silk net extract, Saengyeolgui stem extract, and seaweed extract.

It is characterized in that it further contains Wangbulhyang extract and lignified lignified extract.

2 to 10% by weight of the wild ginseng root culture medium, 1 to 3% by weight of Jeju silk mesh extract, 1 to 3% by weight of Saengyeolgui stem extract, 1 to 3% by weight of seaweed extract, 1 to 3% by weight of Wang Bulhyang extract, It is characterized by being composed of 1 to 3% by weight of lignified extract, 10 to 30% by weight of soybean powder, 10 to 20% by weight of corn powder, 10 to 20% by weight of calcium carbonate, and the balance of rice bran.

According to the feed composition for edible snails using the wild ginseng culture medium of the present invention, the added value of snails can be increased by increasing the initial survival rate of edible snails and increasing the content of chondroitin sulfate contained in edible snails, thereby improving farm income. There is an advantage to being able to contribute.

Hereinafter, the present invention will be described in detail.

The biggest feature of the present invention is that by manufacturing a feed composition for edible snails using wild ginseng culture medium with a high saponin content, it increases the survival rate by increasing the immunity of edible snails, and at the same time, the content of chondroitin sulfate is also increased. .

The feed composition for edible snails according to the present invention is characterized by containing wild ginseng culture medium, rice bran, soybean powder, corn powder, and calcium carbonate.

The wild ginseng root culture medium is a characteristic ingredient of the present invention, and is a culture medium remaining after separating wild ginseng adventitious roots generated during the cultivation of wild ginseng adventitious roots (fine roots) through tissue culture. More specifically, the m-MS medium to which a carbon source is added. It may be a culture filtrate after culturing wild ginseng roots for 50 to 70 days in modified Murashige and Skoog medium, but is not limited thereto. At this time, one or more of sucrose and chitooligosaccharide may be used as the carbon source.

This wild ginseng culture medium contains about 2.3 mg/g of saponin, which can increase the initial survival rate by increasing the immunity of young edible snails, and also increases the chondroitin sulfate content of edible snails. In addition, it also increases the content of various useful ingredients contained in edible snails.

Here, the m-MS medium (modified Murashige and Skoog medium) contains 2290-2294 mg of KNO ₃ , 365-369 mg of MgSO ₄ ·7H ₂ O, 273-277 mg of NH ₄ H ₂ PO ₄ , and FeSO based on 1 liter of suspension culture. ₄ ·7H ₂ O 12~16mg, Na ₂ ·EDTA 17~20mg, CaCl ₂ ·2H ₂ O 181~185mg, Myo-inositol 90~94mg, Niconic acid 4~6mg , Thiamine-HCl 4~6mg, Pyridoxine-HCl 0.4~0.5mg, H ₃ BO ₃ 3~6mg, MnSO ₄ ·H ₂ O 8~10mg, KI 0.5~1.5mg, It may contain 0.5 to 1.5 mg of ZnSO ₄ ·7H ₂ O, 0.1 to 0.3 mg of CuSO ₄ ·5H ₂ O, and 0.05 to 0.15 mg of Na ₂ MoO ₄ ·2H ₂ O, and preferably 2291.67 mg of KNO ₃ , MgSO ₄ · 7H ₂ O 366.67 mg, NH ₄ H ₂ PO ₄ 275.00 mg, FeSO ₄ ·7H ₂ O 13.75 mg, Na ₂ ·EDTA 18.33 mg, CaCl ₂ ·2H ₂ O 183.33 mg, myo-inositol 91.67 mg, niconic acid 4.58 mg , thiamine-HCl 4.58 mg, pyridoxine-HCl 0.46 mg, H ₃ BO ₃ 4.58 mg, MnSO ₄ ·H ₂ O 9.17 mg, KI 0.92 mg, ZnSO ₄ ·7H ₂ O 0.92 mg, CuSO ₄ ·5H ₂ O 0.19 mg , Na ₂ MoO ₄ ·2H ₂ O 0.10 mg, but is not limited thereto. Since this m-MS medium is widely known in the field to which this technology belongs, further description thereof will be omitted.

In addition, the carbon source may be included in an amount of 1 to 5 g based on 1 L of the m-MS medium suspension culture.

The rice bran, soybean powder, corn powder, and calcium carbonate are ingredients commonly used in edible snail feed. The rice bran serves to supply minerals and vitamins, and the soybean powder serves as a protein source to help snails grow. The corn powder serves as a source of carbohydrates to supply energy, and the calcium carbonate is a component of the snail's shell and is a nutrient that requires essential intake.

At this time, the composition ratio is preferably composed of 2 to 10% by weight of the wild ginseng root culture medium, 10 to 30% by weight of soybean powder, 10 to 20% by weight of corn powder, 10 to 20% by weight of calcium carbonate, and the balance of rice bran. Considering the overall nutritional supply, immunity improvement, and chondroitin sulfate content, if the wild ginseng culture medium is less than 2% by weight, the effect of improving immunity and chondroitin sulfate content is minimal, and if it exceeds 10% by weight, economic feasibility and efficiency are reduced. Because you lose.

As described above, raising edible snails using the feed composition of the present invention has the advantage of not only increasing the initial survival rate of the snails, but also increasing the content of chondroitin sulfate, a useful substance.

On the other hand, the feed composition for edible snails of the present invention preferably further includes Jeju silk mesh extract, Saengyeolgi stem extract, and seaweed extract.

Martensia jejuensis is a domestic endemic species growing in Jeju waters belonging to the order of red algae. It has excellent antibacterial activity and is rich in minerals, which can further increase the initial survival rate of edible snails. It also increases the content of chondroitin sulfate in snails.

Rosa davurica is a perennial plant belonging to the Rosaceae family. Its stem extract has excellent antioxidant and anti-inflammatory activities and helps improve immunity, thereby increasing the survival rate of edible snails and promoting growth.

Asparagopsis taxiformis is a species belonging to red algae and is distributed worldwide in tropical and temperate seas. This extract of sea buckthorn not only has excellent antibacterial activity, but is also rich in minerals, which increases the survival rate of edible snails and promotes their growth. Additionally, it increases the content of chondroitin sulfate in edible snails.

In the present invention, the method for producing an extract from the Jeju silk net, the stem of the ginseng tree, or the seaweed is sufficient as long as it is extracted using water as a solvent. This is well known in the field to which this technology belongs, so detailed information about this is required. The explanation is omitted.

It is preferable that the Jeju silk net extract, the stem extract of Saengyeolgui, and the seaweed extract are each included in the range of 1 to 3% by weight in the feed composition. If the content is too small, their role is insignificant, and if the content is excessive, the role is insignificant. Rather, this is because it can inhibit the growth of edible snails.

In addition, it is preferable that the feed composition for edible snails of the present invention further comprises an extract of King Bulpyeong and a lignified extract.

The Melandrii Herba is a seed of Melandrium firmum and Melandrium apricum, which are biennial herbs belonging to the Caryophyllaceae family. The Melandrii Herba extract has excellent immune-boosting and antiviral activities, making it an edible snail. increases the survival rate of

The lignified is a light purple flower of the perennial vine plant Pueraria thunbergina, which has excellent immune-boosting effects and increases the chondroitin sulfate content of edible snails.

In the present invention, the method for producing an extract from the above-described kingbuldung or lignified is sufficient as long as it is extracted using water as a solvent, and since this is well known in the field to which this technology pertains, a detailed description thereof will be omitted.

It is preferable that the Wangbuldong extract and the lignified lignified extract are each included in the range of 1 to 3% by weight in the feed composition. If the content is too small, their role is insignificant, and if the content is excessive, it may actually inhibit the growth of edible snails. Because you can.

In addition, the feed composition for edible snails of the present invention may further include 1 to 2% by weight of mountain snail extract.

Saussurea neoserrata is a Korean species that grows deep in the mountains, and the extract of its young shoots helps the edible snails grow smoothly, allowing adult snails to be eaten more quickly.

In the present invention, the method for producing an extract from the young shoots of the above-mentioned mountain golchwi is sufficient to extract the extract using water as a solvent, and since this is well known in the field to which this technology pertains, a detailed description thereof will be omitted.

Hereinafter, the present invention will briefly describe a method of raising edible snails by feeding the feed composition for edible snails of the present invention.

First, edible snails hatched from eggs are fed the feed composition once every 1 to 2 days and raised for 1 to 3 months to grow into baby snails. At this time, 40 to 60 baby snails are raised per breeding tank, and the feed composition is mixed with water at a weight ratio of about 1:0.5 to 1.5 and fed at about 1 to 2 times the volume of the snail's body size.

Then, the baby snails are divided into different breeding tanks and fed with the feed composition mixed with water once every 2 to 4 days and raised for 4 to 6 months to grow into adult snails.

At this time, adult snails are reared in groups of 15 to 25 per breeding tank, and the feed composition mixed with water is fed at a volume of about 1 to 2 times the size of the snail's body size.

Edible snails raised as described above have the advantage of having a high survival rate and high added value due to their high chondroitin sulfate content.

Hereinafter, the present invention will be described in detail through specific examples.

(Example 1)

A feed composition was prepared by mixing 5% by weight of wild ginseng root culture medium, 20% by weight of soybean powder, 20% by weight of corn powder, 15% by weight of calcium carbonate, and the remainder of rice bran.

At this time, the culture medium for wild ginseng cultured roots was used as a culture medium discarded after culturing and harvesting wild ginseng cultured roots for 60 days in m-MS medium (modified Murashige and Skoog medium) containing a carbon source. The composition of the m-MS medium is as follows. It was the same as Table 1, and 2g of sucrose and 1g of chitooligosaccharide per 1L of culture medium were added as carbon sources and used for culture.

m-MS medium composition (mineral composition based on 1L of culture medium) KNO ₃ 2291.67mg MgSO ₄ ·7H ₂ O 3 366.67 mg NH ₄ H ₂ P O ₄ 275.00mg _FeSO4 · _7H2O 13.75mg _Na2 ·EDTA 18.33mg CaCl ₂ ·2H ₂ O 183.33mg Myo-inositol 91.67mg Nikon Mountain 4.58mg Thiamine-HCl 4.58mg Pyridoxine-HCl 0.46mg _{H3BO3 _} 4.58mg MnSO ₄ ·H ₂ O 9.17mg KI 0.92mg ZnSO ₄ ·7H ₂ O 0.92mg CuSO ₄ ·5H ₂ O 0.19mg Na ₂ MoO ₄ ·2H ₂ O 0.10mg

(Example 2)

The same procedure as in Example 1 was carried out, but 2% by weight of Jeju silk net extract, 2% by weight of Saengyeolgui stem extract, and 2% by weight of seaweed extract were further mixed.

At this time, the Jeju silk mesh extract was used by adding 20 times the weight of water to the entire Jeju silk mesh, extracting at 90°C for 5 hours, concentrating to 20 brix using a rotary vacuum concentrator (EYELA), and freeze-drying. The stem extract of Saengyeolgui was used by adding 20 times the weight of water to the stem of Saengyeolgui, extracting it at 90°C for 5 hours, concentrating it to 20 brix using a rotary vacuum concentrator (EYELA), and freeze-drying. 20 times the weight of water was added to the outcrop of sea buckthorn, extracted at 90°C for 5 hours, concentrated to 20 brix using a rotary vacuum concentrator (EYELA), and freeze-dried for use.

(Example 3)

The same procedure as in Example 1 was carried out, but 2% by weight of Wangbulhyeong extract and 2% by weight of gallflower extract were further mixed.

At this time, the extract of Wangbul Yuhaeng was used by adding 20 times the weight of water to Wang Bul Yuhaeng, extracting it at 90°C for 5 hours, concentrating it to 20 brix using a rotary vacuum concentrator (EYELA), and freeze-drying. 20 times the weight of water was added, extracted at 90°C for 5 hours, concentrated to 20 brix using a rotary vacuum concentrator (EYELA), and freeze-dried for use.

(Example 4)

The same procedure as in Example 2 was carried out, but 2% by weight of Wangbulhyeong extract and 2% by weight of gallflower extract were further mixed.

At this time, the Wangbul Yudong extract and the lignified lignified extract were prepared in the same manner as in Example 3.

(Comparative Example 1)

A feed composition was prepared by mixing 25% by weight of soybean powder, 25% by weight of corn powder, 15% by weight of calcium carbonate, and the balance of rice bran.

(Test Example 1)

Test feed was prepared by mixing the feed compositions prepared in Examples 1 to 4 and Comparative Example 1 with water at a 1:1 weight ratio, and then edible snails were raised using the feed composition.

An appropriate number of snail eggs were transferred to a breeding tank and stored for 14 days to hatch. Then, 50 baby snails hatched in a breeding tank were transferred to another breeding tank and reared for 2 months. At this time, 10 breeding tanks were divided into 5 groups of 2 each, and each group was fed one of the feeds from Examples 1 to 4 and Comparative Example 1 once a day for 2 weeks, and once a day for 8 weeks to raise baby snails. bred. The amount of feed for the test was fed to be about 1.5 times the volume of the snail's body size.

And the survival rates of baby snails were compared and shown in Table 2 below.

survival rate of baby snails division Survival rate (%) Example 1 97.5 Example 2 98.5 Example 3 98.5 Example 4 99.0 Comparative Example 1 78.5

As shown in Table 1, it was confirmed that Examples 1 to 4 of the present invention had superior survival rates of baby snails compared to Comparative Example 1.

Typically, snails have a low survival rate of baby snails and a high survival rate of adult snails, so the survival rate at a young age determines the overall survival rate. Therefore, through this test example, the feed compositions of Examples 1 to 4 of the present invention significantly improved the survival rate of edible snails. I was able to confirm the elevation.

(Test Example 2)

Then, 20 baby snails reared as in Test Example 1 were divided into different breeding tanks, and the same type of test feed as the baby snails was fed once every 3 days, and they were reared for an additional 4 months to raise adult snails. The final size of the edible snail was about 7 to 8 cm.

And the content of chondroitin sulfate was measured from the growth of the final edible snail by spectrometry, and the results are shown in Table 3 below.

Content of chondroitin sulfate division Chondroitin sulfate content (%) Example 1 2.5 Example 2 3.7 Example 3 3.8 Example 4 4.2 Comparative Example 1 1.5

As shown in Table 3, it was confirmed that Examples 1 to 4 according to the present invention had a significantly higher content of chondroitin sulfate than Comparative Example 1.

The description of the present invention described above is for illustrative purposes, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential features of the present invention. will be. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive. For example, each component described as single may be implemented in a distributed manner, and similarly, components described as distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims described below, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention.

Claims (5)

2-10% by weight of wild ginseng culture medium, 1-3% by weight of Jeju silk mesh extract, 1-3% by weight of Saengyeolgui stem extract, 1-3% by weight of seaweed extract, 1-3% by weight of King Bulhyang extract, lignified It consists of 1 to 3% by weight of extract, 10 to 30% by weight of soybean powder, 10 to 20% by weight of corn powder, 10 to 20% by weight of calcium carbonate, and the balance of rice bran.
The wild ginseng culture medium is,
A feed composition for edible snails using wild ginseng culture medium, characterized in that it is a culture filtrate after culturing wild ginseng cultured roots for 50 to 70 days in m-MS medium (modified Murashige and Skoog medium) to which a carbon source is added.
According to paragraph 1,
A feed composition for edible snails using wild ginseng root culture medium, characterized in that it further contains 1 to 2% by weight of Saussurea neoserrata extract. delete delete delete