KR20160014841A - Feed additive using oriental herbal medicine residues and methyl sulfonyl methane - Google Patents

Abstract

The present invention relates to a feed additive including oriental medicine residues and dietary sulfur. More specifically, the feed additive can increase productivity and feed efficiency as well as the growth rate of livestock and improve meat quality without using antibiotics by using byproducts of processed oriental medicine extracts including antler and dietary sulfur. The feed additive with enhanced functionality includes the byproducts of processed oriental medicine extracts including antler and dietary sulfur having a high antimicrobial activity in order to rule out the use of antibiotics, increase the growth rate of livestock, improve meat quality and productivity, and increase the effectiveness of feed.

Description

[0002] FEED ADDITIVE USING ORIENTAL HERBAL MEDICINE RESIDUES AND METHYL SULFONYL METHANE [

The present invention relates to a feed additive using a herbal medicine herb and dihydrogen sulfide, and more particularly, to a herbicide-containing herb extract and a dihydrogen sulfide, And the like.

Domestic brands have been cultivated by focusing on the three major types of beef, pork, and meat, but recently, branding of duck meat, which was classified as other meat, has been rapidly progressing. However, in most brands of meat, the brand name has been applied to marketing by focusing on local specialties (such as cleanliness), environmentally friendly, and feed additive. In particular, after feeding functional ingredients as feed additives, It has been confirmed that it is over-packed and branded as it is. In the case of ducks, the toxic inorganic sulfur is fed in a short time (within one week), or over-packed as if it is fed, and the duck duck (inorganic sulfur) is attracted to the consumer. Similarly, the feed additive is fed for a certain amount, And the environment of the breeding environment are mostly overpacked as if they are effective.

Sulfur is known to be involved in detoxification in animals. Sulfur is mostly composed of synthetic amino acids such as methionine and cysteine. It is synthesized with protein in body. Supply of sulfur to finishing pigs or chickens is effective when it is in the form of amino acid, but sulfuric sulphate has little effect on protein synthesis It can not be recognized, and even if it is absorbed, it is not only excreted in the body but also toxic when it is overdosed, which can be a fatal injury to the human body. In addition, inorganic sulfur released from the body has a disadvantage of causing environmental pollution. Such inorganic sulfur sulfur may cause various disadvantages such as bioavailability in the body, toxicity in the case of overdose, environmental pollution, etc. Therefore, it is preferable to feed organic sulfur in order to improve the bioavailability of inorganic sulfur, The expectation of production of livestock products is increasing.

In addition, as the livestock industry grew rapidly, livestock began to be reared, and when the livestock became immune due to illness, rapid propagation occurred. As a result of overuse of antibiotics to prevent this, side effects of antibiotic-resistant bacteria (super bacteria) have emerged and they cause serious problems when infecting the human body. Therefore, it is urgent to develop an immunity enhancer that can enhance the immunity of such livestock to prevent diseases and improve the breeding performance.

Therefore, the present inventors intend to provide a non-antibiotic-functional feed additive by using the by-product of processing herbal medicine extract containing sulfur and deer antioxidant to improve the morality of the consumer and the morality of the industry and enhance the immunity and the breeding performance of the livestock. Korean Patent No. 10-0470455 (feed containing ginseng saponin and its production method) as a related art for a functional feed additive provides a feed composition for pork containing a ginseng extract, and Korean Patent No. 10-0470455 -0966834 (a method for producing a perilla powder for chicken feed addition, a powder thereby, and a chicken feed using the powder) provide a method for producing a powder for adding chicken feed containing perilla.

Deer antler has been used as a precious medicine in oriental medicine since ancient times as well as ginseng, and Methyl Sulfonyl Methane (MSM) is a component that exists in all living organisms and is naturally produced in animals including humans It is an oxidized metabolite of Dimethyl Sulfoxide (DMSO) containing 34% sulfur and is a bioactive sulfur in natural organic form. It is contained in milk (3.3ppm), coffee (1.6ppm), tomato (0.86ppm), corn (0.11ppm) and alfalfa (0.07pp).

It is an object of the present invention to provide a feed additive which improves the growth rate, meat quality, productivity and feed efficiency of livestock without using antibiotics by using the by-product of extracting herbal medicines containing the antler and dietary sulfur.

In order to accomplish the above object, the present invention provides a feed additive for substituting antibiotics, which contains by-product of herbal medicine extracting processing containing antler.

The medicinal herbs mentioned above may be used as a medicament for preventing and treating cancer and for preventing and treating cancer, Daegu, Daejang, Daejang, Daesan, Daejang, Daejang, Daejung, Daejung, Daejin, Cheonjak, Donggi, Dongjin, Daegu, Daegu It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. , Ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, element, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, Acaricus, acerus, lobster, labyrinth, livestock, abalone, Youngsil, Youngji, Ogapi, Ohma, Omiza, and Ginseng, ginseng, jinsoo, jaehae, peony, god, scorpion, killing, bamboo leaf, bamboo leaf Tea, eucalyptus, chrysanthemum, geocheppy, earth, jisil, jieyu, zhuang, dermis, bloom, green tea, tea electron, Red pepper, pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, Fennel, black bean, and black bean.

The herb extract-containing processing by-product of the antler is contained in an amount of 0.01 to 1.0% by weight based on the feed.

(1) hot water extraction and concentration of herbal medicines; (2) adding the antler extract and the mushroom extract to the concentrate concentrated in the step (1) and concentrating the concentrate; (3) filtering the concentrated liquid concentrated in the step (2), and drying and pulverizing the remaining solids.

In the step (2), the concentrate, the antler extract and the mushroom extract are mixed in an amount of 40 to 60% by weight, 20 to 40% by weight and 5 to 20% by weight, respectively.

Wherein the feed additive is a feed additive for ducks.

The present invention also provides an antibiotic substitute feed additive containing antler extract, herbal medicine extracting by-product and MSM (methyl sulfonyl methane).

The medicinal herbs mentioned above may be used as a medicament for preventing and treating cancer and for preventing and treating cancer, Daegu, Daejang, Daejang, Daesan, Daejang, Daejang, Daejung, Daejung, Daejin, Cheonjak, Donggi, Dongjin, Daegu, Daegu It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. , Ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, element, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, Acaricus, acerus, lobster, labyrinth, livestock, abalone, Youngsil, Youngji, Ogapi, Ohma, Omiza, and Ginseng, ginseng, jinsoo, jaehae, peony, god, scorpion, killing, bamboo leaf, bamboo leaf Tea, eucalyptus, chrysanthemum, geocheppy, earth, jisil, jieyu, zhuang, dermis, bloom, green tea, tea electron, Red pepper, pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, Fennel, black bean, and black bean.

The herb extract-containing processing by-product of the antler is contained in an amount of 0.01 to 1.0% by weight based on the feed.

(1) hot water extraction and concentration of herbal medicines; (2) adding the antler extract and the mushroom extract to the concentrate concentrated in the step (1) and concentrating the concentrate; (3) filtering the concentrated liquid concentrated in the step (2), and drying and pulverizing the remaining solids.

In the step (2), the concentrate, the antler extract and the mushroom extract are mixed in an amount of 40 to 60% by weight, 20 to 40% by weight and 5 to 20% by weight, respectively.

The above formula is characterized in that sulfur is contained in an amount of 0.01 to 0.5% by weight based on the feed.

Wherein the feed additive is a feed additive for ducks.

According to the present invention as described above, it is possible to provide a feed additive that improves the growth rate, meat quality, productivity, feed efficiency, and the like of livestock by adding the by-product of extracting herbal medicine containing antler.

In addition, the present invention has the effect of providing a feed additive having enhanced functionality as well as eliminating the use of antibiotics by adding MSM (methyl sulfonyl methane), which has strong antimicrobial activity, together with a by-product of extracting herbal medicine containing antler .

In addition, the present invention can produce meat products such as functional ham, sausage and the like by feeding the antler biologically active feed additive, which is added with the antler extract and the by-product of the herb extract and the dietary sulfur at an optimum mixing ratio, to the animal.

Fig. 1 is a manufacturing process diagram of duck meat. Fig.
Fig. 2 is a manufacturing process diagram of duck meat sausage. Fig.
FIG. 3 shows the DPPH and alkyl radical scavenging activities of the byproducts of herbal medicine extracts containing antler.
FIG. 4 shows antioxidant enzyme activity in the blood of ducks fed with natural plant sulfur (MSM) and antler extracts containing herbal extracts.
FIG. 5 shows the sulfur content of duck meat fed with natural plant sulfur (MSM) and byproducts containing antler extracts.
6 shows changes in water holding capacity and heat loss of duck meat fed with natural plant sulfur (MSM) and antler extracts of herbal medicine extracts.
FIG. 7 shows the change in storage loss of duck meat fed with natural plant sulfur (MSM) and antler processing herbal medicine extract byproducts.
8 shows changes in pH of duck meat fed with natural plant sulfur (MSM) and antler extracts containing herbal extracts during storage.
Fig. 9 shows sensory evaluation of duck meat fed with natural plant sulfur (MSM) and antler processing herbal medicine extracts by-products.

Hereinafter, the present invention will be described in detail.

The present invention provides a feed additive containing a herb-containing herbal extract processing by-product.

Further, the present invention provides a feed additive containing herbal medicine extractive processing by-products containing deer antler and MSM (methyl sulfonyl methane).

The feed additive is an animal feed additive. In the present invention, the animal includes mammals such as pigs, cows and goats, fish such as carp and goldfish, and poultry such as pheasants, chickens, ducks and turkeys.

The medicinal herbs mentioned above may be used as a medicament for preventing and treating cancer and for preventing and treating cancer, Daegu, Daejang, Daejang, Daesan, Daejang, Daejang, Daejung, Daejung, Daejin, Cheonjak, Donggi, Dongjin, Daegu, Daegu It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. , Ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, element, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, Acaricus, acerus, lobster, labyrinth, livestock, abalone, Youngsil, Youngji, Ogapi, Ohma, Omiza, and Ginseng, ginseng, jinsoo, jaehae, peony, god, scorpion, killing, bamboo leaf, bamboo leaf Tea, eucalyptus, chrysanthemum, geocheppy, earth, jisil, jieyu, zhuang, dermis, bloom, green tea, tea electron, Red pepper, pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, Fennel, black and black, and the kind of herbal medicine is not limited thereto.

It is preferable to use processing by-products obtained from at least one medicinal herb selected from the group consisting of antler, hwangjeong, hwanggi, angelica, mantis, gugija, licorice, cinnamon and safflower.

The by-product of herbal medicine extracting processing containing antler is an herbal medicine herb which is a solid material remaining after extracting the herbal medicine as a raw material, and important components remaining in the above-mentioned herbal medicine processing by-products are more important than the herbal medicine extract. According to the present invention, the by-product of extracting herbal medicine containing antler according to the present invention can be prepared by subjecting the herbal medicine containing antler to hot water extraction and pulverizing the remaining solids and extracting the herbal medicine containing antler by any method.

According to one embodiment of the present invention, the herbal medicine extractive processing by-product containing the antler contains the extract of the herbal medicine and the concentrate, and then adding the antler extract and the mushroom extract to the concentrate at a ratio of 2: 1 to 6: 1, preferably 4: To 6: 1, and the mixture is concentrated at 80 to 90 ° C., filtered and then pulverized to obtain a solid. The mushroom is preferably prepared by crushing the above-mentioned mushroom, It is preferably one or more selected from the group consisting of Chinese cabbage. At this time, it is preferable that the concentrate, the antler extract, and the mushroom extract are mixed at 40 to 60 wt%, 20 to 40 wt%, and 5 to 20 wt%, respectively, in order to improve the functionality such as the growth rate, meat quality, productivity and feed efficiency of the livestock.

It is preferable that the by-product of extracting herbal medicine containing the antler contains 0.01 to 1.0 wt% of the feed, and the dietary sulfur is preferably contained in an amount of 0.01 to 0.5 wt% with respect to the feed.

By adding the by-product of extracting herbal medicines and dietary sulfur containing antler in the same range as above, it is possible to feed diets with improved economic efficiency and feed efficiency without side effects, more specifically, by adding an appropriate amount of expensive herbal medicines Increase price competitiveness and increase the livestock growth rate, meat quality, productivity and And the feed efficiency and other functionalities can be improved.

The present invention also provides a method for producing duck meat, comprising the steps of: (1) mixing duck meat and drench solution; (2) filling the mixture in the casing; (3) drying the filler in a fumed smoke and smoking and then heat-treating the feed, wherein the duck meat comprises 0.01 to 0.5% by weight of dietary sulfur and 0.01 to 1.0% by-product of herbal medicine extract, % Of a duck meat product obtained from a duck produced by feeding a feed containing the duck meat product.

In the step (1), it is preferable that the saline solution contains water, salt, emulsifier, antioxidant, sugar, millet and spice.

In the step (2), it is preferable that the casing is a bamboo, hog, collagen, cellulose, polyvinylidene chloride or fibrin.

In the step (3), it is preferable that the packing is dried and smoldered in a hot smoke, and then heated at 65 to 85 ° C for 30 to 40 minutes so that the center temperature of the casing reaches 70 to 75 ° C.

In addition, the filling material heat-treated in the step (3) is preferably cooled in a low-temperature room of 4 to 10 ° C for 30 minutes by lowering the temperature by showering with cold water for 3 minutes.

The duck meat product thus prepared has high sulfur and amino acid content and low fatty acid content of duck meat, and has high flavor, taste, chewiness, juiciness and overall acceptability, thus providing a functional herbal sulfur duck meat product having excellent commercial properties have.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Example 1. Breeding of functional antibiotic-free herbal sulfur ducks

The feed additive was added as a basic feed to the chicks of Dong-A Won and the duck feed for the cultivation for 6 weeks. The farms were used to treat 100 chickens (two repeats of each treatment) The feed and water were fed unlimitedly and the light was turned on for 18 hours.

The control group was a feed test group with no added natural herbal sulfur (MSM) and antler extract, 0.03% of natural plant sulfur, 0.03% of natural plant sulfur, and 0.5% In the feed test.

The dietary sulfur in this example was the natural vegetable organic sulfur (MSM) extracted from the lignin of the pine tree, and the optimum content of 0.03% in the feed was derived from the preliminary experiment.

In addition, about 30% or more of physiologically active ingredients such as ganglioside and IGF-1 remained in the by-product of herbal medicine extraction using hot water. The medicinal herbs consisted of Hwangjeong, Hwanggi, Angelica gigas, Gyungguk, Gugija, Chrysanthemum, Licorice, Cinnamon and Sukjip. Specifically, the medicinal herb was heated and mixed with water in a mixed extractor to extract and concentrate the extract to a solid content of 15%, and then the extracts of the antler extract and the extract of Mulberry mushroom were added to the extract solution until the solid content became 10% The mixture was concentrated and filtered. The grapefruit seed extract was added to the filtrate, and the mixture was concentrated to a solid content of 8% at 85 캜. Thereafter, the concentrate was filtered, and the remaining solids were pulverized to prepare a herbal medicine extractive processing by-product containing antler.

Example 2. Preparation of duck meat

The duck flesh was cut into 2.5 cm ² squares, and then mixed with the drench solution prepared previously. The mixing was carried out for about 20 minutes. After the mixing was completed, the mixture was mixed in a blender for about 2 hours, and after the mixing was completed, the mixture was filled into a fused casing. After charging, fumigation was carried out with a smoke, and the heating conditions were such that the internal temperature was 72 ° C and the heated product was cooled with cooling water.

Example 3: Preparation of duck meat sausage

Duck meat smoked sausage was processed by using mulberry meat. The processed sausage dough was filled into a cellulosic casing and smoked by using a smoke. The sample was heated to an internal temperature of 72 ° C and a final internal temperature of 160.8 ° F 71.7 < 0 > C). Immediately after heating, samples were cooled in ice to prevent microbial growth, and each sample was stored under refrigeration conditions at 4 ° C.

EXPERIMENTAL EXAMPLE 1. Antioxidative Effectiveness of By-product of Herb Extracted by Drug Using Electron Spin Resonator (ESR)

(1) DPPH radical scavenging ability

DPPH radical scavenging activity was measured by adding 60 μl DPPH solution (60 μM) to a 60 μl sample solution, stirring for 10 seconds, and transferring the mixed solution to a quartz capillary tube 2 minutes later by ESR. The spectra were recorded under the conditions of field: 337.1 ± 5 mT, power: 4 mW, scan time: 2 min, amplitude: 1 × 500 and time constant: 0.3 s. The DPPH radical scavenging activity Respectively.

DPPH radical scavenging ability (%) = (100 - (ESR signal intensity for sample / ESR signal intensity for control)) x 100

(2) Alkyl radical scavenging ability

Alkyl radical scavenging activity was determined by the addition of 10 mM AAPH (2-aminopropane) hydrochloride, 10 mM 4-POBN (α- (4-pyridyl-1-oxide) -Nt-bytylnitrone) The phosphoric acid buffer solution (pH 7.4) was incubated at 37 ° C for 30 minutes, and the mixed solution was transferred to a quartz capillary tube and measured by ESR. The spectra were recorded under the conditions of field: 337.1 ± 5 mT, power: 4 mW, scan time: 2 min, amplitude: 1 × 500 and time constant: 0.3 s. Respectively.

Alkyl radical scavenging ability (%) = (100 - (ESR signal intensity for sample / ESR signal intensity for control)) x 100

Experimental Example 2. Characterization of sulfur sausage and sulfur duck meat sausage

(1) Breeding performance

From the time immediately after embedding, 3.5kg of fresh weight was bred until delivery, and the initial weight of all treatments was similar.

Daily feed intake (kg) = (Feeding amount for 10 days - Amount of remaining feed) / Number of animals per experimental group × 10 days

Weight gain per day (kg) = (Weight after experiment - Weight before experiment) / Number of animals per experiment × 10 days

Feed efficiency (%) = daily gain / daily feed intake

Mortality (%) = (Number of standing breeding - Number of our ducks) / 100

(2) Analysis of blood and antioxidant enzyme activity in blood

At the end of the experiment, 10 blood samples were collected from each treatment group, and total cholesterol was measured by enzyme-colorimetric assay kit (AM 202-K, Asan Pharma) using the separated serum. Protein, albumin, aspartate aminotransferase ), alanine transaminase (ALT), and blood urea nitrogen (BUN) were measured.

Catalase activity was measured using a Catalase assay kit from Sigma. That is, a sample was added to an eppendorf tube, and 25 μl of a substrate solution for colorimetric analysis was added to a total volume of 75 μl using a phosphate buffer solution. Each tube was inverted and mixed, and then left at room temperature for 5 minutes 900 [mu] l of stop solution was added. 10 μl of the solution was transferred to a new eppendorf tube and added with 1 ml of the color-forming solution. After mixing, the solution was allowed to stand at room temperature for 15 minutes and the OD value was measured at 520 nm.

Superoxide dismutase activity was measured using a Superoxide Dismutase assay kit from Fluka. That is, 2 μl of each sample was added to 96-well tubes containing sterilized distilled water, WST solution, and enzyme-active solution, or 2 μl of GST control was added to the enzyme-corrected group, and the absorbance was measured at 450 nm .

(3) pH and general components

10 g of the sample was diluted in 90 mL of distilled water and the average value was determined by repeating the measurement five times with a pH meter (Mettler-Toledo, Model 340, Schawarzenbach, Switzlerland) and the content of water, fat and protein was measured according to the AOAC .

(4) Water holding capacity (WHC)

The amount of water liberated from the sample according to the method of Jauregui et al. (1981, A simple centrifugal method for measuring expressible moisture, a water-binding property of muscle foods. J. Food Sci., 46: 271-273) EM) were measured and expressed as percent (%).

(5) Meat color

Hunter L (lightness, brightness), a (redness, redness) and b (yellowness, yellowness) were measured five times each using Chroma Meter (CR-10, Minolta Corporation, Respectively.

(6) Storage loss and heating loss

The weight before storage and the weight during storage are shown in percent (%). The weight before heating and the weight after heating are shown in percent (%) after heating.

(7) Thiobarbituric acid reactive substances (TBARS) value

The TBARS values for measuring the fatty acid composition were determined by the method of Sinnhuber and Yu (1977, The 2-thiobarbituric acid reaction, an objective measure of the oxidative deterioration occurring in fats and oils. J. Jap. Oil Chem. Soc. 26: 259-267) And expressed as the amount of malonaldehyde (mg) per kilogram.

(8) Volatile sulfur compound analysis

Analysis of the volatile sulfur compounds from the samples was carried out by solid-phase microextraction (SPME) method. The compounds were separated by using a gas chromatograph (GC) equipped with a 75 ㎛ Carboxen-PDMS (polydimethylsiloxane) paper with a flame ionization detector and subjected to qualitative analysis using a GC-mass spectrometer (MS). 200 ppm chlorobenzene was used as an internal standard for quantitative analysis of sulfur compounds.

(9) Amino acid content analysis

First, the sample was subjected to acid stabilization pretreatment. That is, about 0.08 g was weighed, and 10 ml of 6N HCl was added thereto, followed by mixing and replacing with nitrogen, followed by sealing and hydrolysis at 110 ° C for 24 hours. The hydrolyzate was filtered (0.45 nm syringe filter, whatman), filtered under reduced pressure at 55 to 60 ° C and diluted to 10 ml with 0.2 N sodium citrate buffer (pH 2.2). The instrument was analyzed by Ninhydrin method at 440nm and 570nm by injecting 20μg into capsules using an automatic amino acid analyzer (L-8500A, Hitachi).

(10) Fatty acid composition analysis

Total lipid extraction was performed with a sample in a tube and Folch method (1957, A simple method for isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226: 497-509). Thereafter, methylation was performed for 1 hour in a water bath of 100 ° C, and after cooling, hexane was added to separate the layers, and then the upper layer was taken. It was then analyzed using gas chromatography (HP 5890II, Hewlett Packard Co.) equipped with a capillary column (100 m × 0.25 mm id × 0.20 μm film thickness), using nitrogen as the carrier gas and measuring the initial temperature (180 deg. C), and the final temperature was 240 deg. C (2 deg. C / min). The temperature of injector and detector was set at 250 ℃.

(11) Statistical analysis

The results obtained from the experiment were analyzed by SAS / PC (SAS, 1997) and Duncan 's multiple test.

(12) Sensory characteristics analysis

In order to evaluate the change of sensory characteristics from the viewpoint of the correct consumer, 50 people were randomly selected and sensory evaluation was performed according to storage period. Before the sensory evaluation, only the basic methods of the evaluation items were explained, and the evaluation was performed randomly according to temperature and site. Fresh meat investigated aroma, color, off-flavor, drip and acceptability, and meat products were analyzed for flavor, juiciness, Year, tenderness, and acceptability.

Experiment result.

(1) Antioxidant efficacy of byproducts of antler extract

In the case of byproducts containing antler extracts, the residual efficacy is considerable. Particularly, about 30% or more of physiologically active ingredients such as ganglioside and IGF-1 remain in the antler processing by-products extracted by hot water. Nitrogen in DPPH easily accepts electrons and decolorizes the original deep purple color, so that the electron donating ability of the antioxidant can be measured. Thus, were an electron spin resonance device, a result of analysis using a (ESR) The IC ₅₀ value of 50% inhibitory concentration for radical generation appeared to 110.05 ㎕ / ml when the alkyl radical, in 134.14 ㎕ / ml for DPPH radical (Fig. 3).

(2) Growth ability of ducks fed by-products of natural plant sulfur (MSM) and antler extracts

The daily feed intake was higher in the treatments 1 and 2 than in the control, and there was no statistical difference in the treatments. The daily gain was slightly higher in the control than in the control. On the other hand, the feed efficiency was higher than that of the control. In particular, the feed efficiency of the herbal supplement 2 was higher than that of the control. On the other hand, the mortality rate of the control group was the highest at 4.5%, followed by the treatment group 1 (3.3%) and the treatment group 2 (2.1%).

(3) Blood characteristics of ducks fed by-products of natural plant sulfur (MSM) and antler extracts

The total protein, albumin and total cholesterol contents tended to increase with the duration of breeding, but there was no statistically significant difference between the groups. AST and ALT are important indicators for the diagnosis of heart or liver disease. When liver cells are damaged, these enzymes are released into the bloodstream and the concentration of these enzymes is increased. The concentration of AST and ALT showed no significant difference between the control group and the combination of the natural plant sulfur and the antioxidant extracts. .

(4) Antioxidant enzyme activities in blood of ducks fed by-products of natural plant sulfur (MSM) and antler extracts

As shown in FIG. 4, the activity of catalase enzyme was increased with the growth of ducks in the catalase enzyme activity, and the activity of catalase enzyme in the ducks fed with natural plant sulfur and antler extracts And showed higher activity than the control. In addition, the activity of superoxide dismutase (SOD) enzyme activity was similar to that of catalase enzyme activity, but the activity increased according to the growth of duck and the addition of natural plant sulfur and deer antler extracts, Lt; / RTI >

(5) Meat of duck meat fed byproducts of natural plant sulfur (MSM) and antler extracts

There was no significant difference in the L value (lightness) between the groups, but the a value (redness) was higher in the control than in the control. Especially, the a value (redness) and b value (yellowness) of duck meat fed with MSM and herbal medicine byproducts were the lowest values.

(6) Sulfur content of duck meat fed byproducts of natural plant sulfur (MSM) and antler extracts

As shown in FIG. 5, there was no difference in the sulfur content in the duck meat among treatments 1 fed with the control and MSM, whereas in the group fed with the MSM and herbal extract processing by-products, the sulfur content was 0.27% Respectively.

(7) Water holding capacity, heat loss and storage loss change of duck meat fed byproducts of natural plant sulfur (MSM) and antler extracts

The percentages of water holding capacity (control: 39.11%, treatment 1: 40.19%, treatment 2: 40.57%) were statistically significant (p <0.05) But there was no significant difference. This indicates that the benefits of Chinese herbal extracts and natural plant sulfur do not significantly affect the processing aptitude of duck meat.

On the other hand, there was no significant difference in the storage loss of duck meat during storage for 3 weeks at 4 ℃. However, as the storage period increased, the storage loss tended to increase in all groups. The storage loss of treatment 2, which was accompanied by MSM and herbal medicine byproducts, was low.

(8) Amino acid content in duck meat fed byproducts of natural plant sulfur (MSM) and antler extracts

The total amino acid content of each group was 42.05 ㎍ / mg for control, 37.91 ㎍ / mg for treatment 1 and 56.70 ㎍ / mg for treatment 2, and the total amino acid content of duck meat The highest value was shown. Especially, in the case of duck meat fed with MSM and herbal extracts, the total amino acid content of duck meat including glutamic acid, cysteine and methionine, which are related to meat taste, was higher than other groups. On the other hand, total amino acid content of MSM - only group showed the lowest value, suggesting that the supply of byproducts of antler containing herb extracts affects the amino acid composition of duck meat.

(9) Fatty acid content in duck meat fed byproducts of natural plant sulfur (MSM) and antler extracts

(C14: 0), palmitic acid (C16: 0), linoleic acid (C18: 2n6), and γ-linoleic acid (C18: 0) were the major fatty acids of duck meat fed with MSM and antler extract : 3n6) and arachidonic acid (C20: 4n6) were higher than those of control and treatment 1. Especially, polyunsaturated fatty acid (PUFA) content was the highest value of 18.15% in duck meat fed MSM and antler extracts.

(10) pH change of duck meat fed byproducts of natural plant sulfur (MSM) and antler extracts

The pH of the duck meat, duck skin, duck meat and skin were measured by storage at 4 ℃ for 30 days. The pH of the duck meat, duck meat and skin was ranged from 7.6 to 7.8 irrespective of the meat and shell. In the case of mixed duck meat and duck meat and bark, the pH decreased with the lapse of storage period, but it increased again after 15 days of storage, while in case of duck bark with high fat content, And MSM-fed group showed the greatest decrease in pH.

(11) Changes in fatty acid groups (TBARS) of duck meat fed byproducts from natural plant sulfur (MSM) and antler extracts

In general, the TBARS value tends to increase as the storage period of food is lengthened. In this experiment, TBARS value increases with storage period. Especially, the highest fatty acid value in the shell of high fat content and the lowest fatty acid value in the duck meat in storage period. As a result of the treatments, it was found that the fatty acid group values of MSM alone (treatment 1) or in the group treated with MSM and antler extracts (treatment 2) were significantly decreased I could. This indicates that the byproducts of MSM and antler extracts can inhibit the fatty acidosis of duck meat.

(12) Sensory evaluation of duck meat sausages with natural product sulfur (MSM) and antler extracts

The results of sensory evaluation of control 1 and control 1 showed similar results. On the other hand, the results of sensory evaluation of duck meat fed with MSM and antler - containing herbal medicine extract by - products showed aroma, , Tenderness, juiciness, and overall acceptability were higher than those of the control and treatment groups. This suggests that the overall preference of processed foods using duck meat can be increased when the MSM and deer antler extracts are fed in parallel.

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (13)

An antibiotic substitute feed additive containing antler extract and herbal medicine extract processing by - products.
The method according to claim 1,
The medicinal herbs mentioned above may be used as a medicament for preventing and treating cancer and for preventing and treating cancer, Daegu, Daejang, Daejang, Daesan, Daejang, Daejang, Daejung, Daejung, Daejin, Cheonjak, Donggi, Dongjin, Daegu, Daegu It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. , Ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, element, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, Acaricus, acerus, lobster, labyrinth, livestock, abalone, Youngsil, Youngji, Ogapi, Ohma, Omiza, and Ginseng, ginseng, jinsoo, jaehae, peony, god, scorpion, killing, bamboo leaf, bamboo leaf Tea, eucalyptus, chrysanthemum, geocheppy, earth, jisil, jieyu, zhuang, dermis, bloom, green tea, tea electron, Red pepper, pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, Fennel, black bean, and black bean. &Lt; RTI ID = 0.0 &gt; 8. &lt; / RTI &gt;
The method according to claim 1,
The feed additive for substituting antibiotics according to claim 1, wherein the by-product of extracting herbal medicine containing antler is contained in an amount of 0.01 to 1.0% by weight based on the feed.
The method according to claim 1,
Wherein the antler extract-containing processing by-product is prepared by the following method.
(1) hot water extraction and concentration of herbal medicines;
(2) adding the antler extract and the mushroom extract to the concentrate concentrated in the step (1) and concentrating the concentrate;
(3) filtrating the concentrated liquid concentrated in the step (2), and drying and pulverizing the remaining solids.
5. The method of claim 4,
Wherein the concentrate, the antler extract and the mushroom extract are mixed in an amount of 40 to 60% by weight, 20 to 40% by weight and 5 to 20% by weight in the step (2), respectively.
The method according to claim 1,
Wherein the feed additive is a feed additive for ducks.
An antimicrobial substitute feed additive containing antler extract, herbal medicine extract processing by-products and sulfur (MSM, methyl sulfonyl methane).
8. The method of claim 7,
The medicinal herbs mentioned above may be used as a medicament for preventing and treating cancer and for preventing and treating cancer, Daegu, Daejang, Daejang, Daesan, Daejang, Daejang, Daejung, Daejung, Daejin, Cheonjak, Donggi, Dongjin, Daegu, Daegu It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. It is a kind of vitamins and minerals. , Ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, element, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, ginseng, Acaricus, acerus, lobster, labyrinth, livestock, abalone, Youngsil, Youngji, Ogapi, Ohma, Omiza, and Ginseng, ginseng, jinsoo, jaehae, peony, god, scorpion, killing, bamboo leaf, bamboo leaf Tea, eucalyptus, chrysanthemum, geocheppy, earth, jisil, jieyu, zhuang, dermis, bloom, green tea, tea electron, Red pepper, pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, red pepper, Fennel, black bean, and black bean. &Lt; RTI ID = 0.0 &gt; 8. &lt; / RTI &gt;
8. The method of claim 7,
The feed additive for substituting antibiotics according to claim 1, wherein the by-product of extracting herbal medicine containing antler is contained in an amount of 0.01 to 1.0% by weight based on the feed.
8. The method of claim 7,
Wherein the antler extract-containing processing by-product is prepared by the following method.
(1) hot water extraction and concentration of herbal medicines;
(2) adding the antler extract and the mushroom extract to the concentrate concentrated in the step (1) and concentrating the concentrate;
(3) filtrating the concentrated liquid concentrated in the step (2), and drying and pulverizing the remaining solids.
11. The method of claim 10,
Wherein the concentrate, the antler extract and the mushroom extract are mixed in an amount of 40 to 60% by weight, 20 to 40% by weight and 5 to 20% by weight in the step (2), respectively.
8. The method of claim 7,
Wherein the dietary sulfur is contained in an amount of 0.01 to 0.5% by weight based on the feed.
8. The method of claim 7,
Wherein the feed additive is a feed additive for ducks.

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