KR101931350B1 - Feed composition for freshwater eels - Google Patents

Abstract

The present invention relates to feed composition for eel cultivation, and more particularly, the composition contains, 100 parts by weight of a compounded feed, 1 to 4 parts by weight of a Pyrus communis flower extract, 1 to 4 parts by weight of an Artemisia fukudo extract, 1 to 4 parts by weight of a Carthamus tinctorius seed extract, 1 to 4 parts by weight of Rubus coreanus, and 1 to 4 parts by weight of a Dypsis lutescens extract. According to the present invention, the growth of eel is promoted, an immune function is improved to prevent mass death, the oily and greasy taste unique to the eel is alleviated and fishy smell can be reduced to improve the sensual preference.

Description

{Feed composition for freshwater eels}

The present invention relates to feed composition for eel cultivation, and more particularly to a feed composition for eel culturing which can promote eel growth, enhance immunity, and improve meat quality and taste.

Eel is a high-protein stamina food, a report of vitamin A, and is also a comprehensive nutritional supplement in summer. In addition, the fat of the eel contains 1,332 mg of DHA, 742 mg of EPA and Lecithin. The lecithin is a major constituent of the cell membrane of the human body and is an indispensable substance in brain cells.

The eel is a specific species that is divided into several stages of growth and can not be artificially scattered and hatching. Due to its physiological characteristics, it is necessary to arrange the middle stage of the growth and arrange it in a suitable environment. However, there are many cases in which aquaculture eel is killed due to various infectious diseases such as bacterial diseases and parasitic diseases caused by the mass colonization in the general aquaculture process from the above-mentioned mounting fishes to edible eels.

In order to prevent this, antibiotics for bacterial diseases are added to the feed or added to the water of the farm for the purpose of relieving various fish diseases at the aquaculture site, but they are not effective for infectious diseases caused by pathogens such as viruses and some parasites. Even if extreme physical methods such as water temperature control and fresh water are used, they can not effectively cope with the prevention and treatment of infection.

In order to overcome these disadvantages, Korean Patent No. 10-0585500 proposes a feed additive for freshwater eel containing a chitosan oligosaccharide derivative obtained by hydrolyzing a mixture of chitosan and Ganoderma lucidum extract. However, these feed additives have a disadvantage in that it is difficult to mass-produce them because they are relatively expensive.

Korean Patent No. 10-1684462 proposes a feed additive for eel cultivation in which eelgrass and stevia or stevioside are mixed at a certain ratio, thereby promoting the growth of eels. However, in order to prevent eelgrass growth caused by infectious diseases I could not.

Korean Patent No. 10-1299537 has proposed a feed additive for eel culturing which contains dry powder of a ginseng root as an active ingredient, but it is disadvantageous in that it is difficult to mass-produce it.

KR 10-0585500 B1 KR 10-1684462 B1 KR 10-1299537 B1

Accordingly, it is an object of the present invention to provide a feed composition for eel culturing which promotes growth of eel and improves immunity.

It is another object of the present invention to provide a diary composition for eel cultivation which improves the taste of the eel, which is peculiar to the eel and improves oily taste and reduces fishy smell.

In order to accomplish the above object, the present invention provides a feed composition for eel culturing, comprising 100 parts by weight of a compound feed, 1 to 4 parts by weight of a Pyrus communis flower extract, 1 to 4 parts by weight of a mushroom extract, 1 to 4 parts by weight of a berry extract of strawberry, and 1 to 4 parts by weight of an extract of Areca larvae.

Wherein the extract is selected from the group consisting of water, ethanol, and a mixture thereof, and the extract is obtained by extracting the extract from the marsh mallow extract by using water, ethanol or a mixture thereof. The extract is obtained by extracting the seed fruit of safflower with water, ethanol, or a mixture thereof, and the berry extract is obtained by extracting the fruit of the berry fruit with water, ethanol, or a mixture thereof, as a solvent , And the Areeka extract is characterized in that the leaf of Areeka is extracted with water, ethanol or a mixture thereof.

And 1 to 4 parts by weight of a fermented extract of Areeka, wherein the fermented extract of Areeka is fermented and extracted by inoculating mycelia with a mushroom mycelium on leaves of Areca.

And 0.5 to 2 parts by weight of aged grapefruit. The grapefruit-aged grapefruit is characterized in that the grapefruit is mixed with sugar in a weight ratio of 1: 0.1 to 0.2 and then aged at 10 to 25 DEG C for 30 to 60 days.

According to the present invention, there is an advantage that the growth of eel is promoted, the immune function is improved, and the group mortality can be prevented. In addition, there is an advantage that the sensory preference can be improved because it is possible to improve the taste of the eel and to improve the oily taste and reduce the fishy smell.

Hereinafter, the present invention will be described in detail.

The feed composition for eel culturing of the present invention comprises 100 parts by weight of a compound feed, 1 to 4 parts by weight of a Pyrus communis flower extract, 1 to 4 parts by weight of a mugwort extract, 1 to 4 parts by weight of a safflower seed extract, And 4 to 4 parts by weight of Areeka extract, thereby promoting the growth of the eel and improving the meat quality and taste while improving immunity.

Here, the term "eel" refers to a freshwater eel that can be ordinarily cultured, and the type of the freshwater eel is not limited.

Also, in the present invention, the feed composition for eel culturing may be a powdery formulation or a pellet formulation, but is not limited thereto.

Examples of the feed include fish meat, barley or wheat flour, seaweed powder and fish oil, more specifically, fish meat 53 to 70% by weight, barley or wheat flour 14 to 20% by weight, 20 to 20% by weight of seaweed powder, 14 to 20% by weight of seaweed powder and 2 to 7% by weight of fish oil. It is of course also possible to further include known raw materials such as potato starch, tapioca starch, vitamins, minerals, calcium, and choline chloride.

Hereinafter, the feed composition of the present invention is based on 100 parts by weight of the compound feed.

The above-mentioned extract of Western blossom means that the Western blossom is extracted with a solvent and filtered. The extract of Western blossom promotes the growth of the eel and improves the immunity, It improves oily taste and reduces fishy smell. More specifically, the extract of Western blossom is obtained by adding 1 to 10 times by weight of a solvent of water, ethanol or a mixture thereof to western blossoms, extracting the mixture at 20 to 90 ° C for 6 to 3 days, and then filtering. In addition, drying and pulverization processes may be added as needed.

Pyrus Communis (Rosaceae) is a Rosaceae plant that has been modified from lowland areas in summer with wild boars from central Europe to Turkey. Leaves are egg-shaped or long oval-shaped ovate with thin petiole. The fruit is usually a bottle-shaped cone, but has many variations. After maturity, eat again after a long time. It has few stony cells and has excellent aroma and taste. It is the most dominant variety in the world today.

If the content of the extract is less than 1 part by weight, promotion of growth of the eel and immune enhancement are not sufficiently attained. If the amount of the extract is more than 4 parts by weight, This is because the amount can be reduced.

The extract of the mugwort extract means that the mugwort is extracted with a solvent and filtered. The mugwort extract enhances the immunity of the eel through the extract of the mugwort, improves the eel-specific taste, improves the oily taste and reduces the fishy smell. More specifically, the extract of the mugwort is obtained by adding 1 to 10 times by weight of a solvent of water, ethanol or a mixture thereof to the mugwort, extracting the mugwort at 20 to 90 ° C for 6 hours to 3 days, and then filtering. In addition, drying and pulverization processes may be added as needed.

The japonica is also known as the mackerel. It grows in tidal flats on the beach or in a place where it is submerged in seawater at high tide. It is known to have excellent ability to inhibit the proliferation of inflammatory diseases and pathogenic bacteria.

If the content of the extract is less than 1 part by weight, the immune enhancement of the eel is not sufficiently achieved. If the amount of the extract is more than 4 parts by weight, the amount of the extract may be reduced It is because.

The safflower seed extract means that the fruit of the safflower (seed fruit) is extracted and filtered using a solvent, and the growth of the eel can be significantly promoted through the extract of the safflower seed. More specifically, the safflower seed extract is prepared by adding 1 to 10 times by weight of a solvent of water, ethanol or a mixture thereof to the fruit of safflower, that is, safflower, extracting it at 20 to 90 ° C for 6 hours to 3 days, will be. In addition, drying and pulverization processes may be added as needed.

Safflower (Carthamus Tincorius L) is a perennial herbaceous plant of the dicotyledonous bellflower of the dicotyledonous plant. It is also called red lantern, itch, and itch. The safflower, which is the seed fruit of the safflower, is squeezed with oil and used for cooking with kerosene. As described above, the safflower seed extract in the present invention means the safflower seed extracted from the safflower which is the fruit (seed fruit) of safflower.

The safflower seed extract is preferably contained in an amount of 1 to 4 parts by weight in the feed composition of the present invention. If the amount of the safflower seed is less than 1 part by weight, it is difficult to promote the growth of eelgrass. If the amount is more than 4 parts by weight, to be.

The bokbunja strawberry extract means that the fruit of bokbunja strawberry is extracted with a solvent and filtered. The extract of the bokbunja strawberry enhances the immunity of the eel, enhances the characteristic of the eel, improves the oily taste, I will. More specifically, the extract of bramble berries is prepared by adding 1 to 10 times by weight of a solvent selected from the group consisting of water, ethanol and a mixture thereof to the brambles of bramble berries, extracting the mixture at 20 to 90 ° C for 6 hours to 3 days, It is. In addition, drying and pulverization processes may be added as needed.

Bokbunja berries brighten the eyes and are effective on the tongue.

The extract of the bramble strawberry is preferably contained in the feed composition of the present invention in an amount of 1 to 4 parts by weight. If the content of the extract is less than 1 part by weight, the effect is insignificant. If the content is more than 4 parts by weight, Because.

The Areeka extract means that the leaf of Areeka is extracted with a solvent and filtered, and the immunity of the eel is improved through the Areeka extract. More specifically, the extract of Areeka is obtained by adding 1 to 10 times by weight of a solvent of water, ethanol or a mixture thereof to the leaves of Areeka, extracting the mixture at 20 to 90 ° C for 6 hours to 3 days, . In addition, drying and pulverization processes may be added as needed.

Areca palm (Chrysalidorcarpus lutescens) is an air purifying plant, a kind of coconut palm, with relatively thin and smooth stems, green to yellowish green, with small leaves facing each other, and the axis of the leaves is yellow with black spots. The pollutants absorbed on the leaves are used as metabolites of plants, some of them are removed and some of them are moved to roots and used as a nutrient source of soil microorganisms. In the case of formaldehyde, it is absorbed by plants through pores of leaves, then converted to formic acid and carbon dioxide in turn, and is used as photosynthetic metabolite through photosynthesis, and its toxicity is eliminated.

Preferably, the extract of Areeka is contained in 1 to 4 parts by weight in the feed composition of the present invention. If the content is less than 1 part by weight, it is difficult to improve the immunity of the eel, and if it exceeds 4 parts by weight, Because.

The feed composition for eel culturing as described above has an advantage that it promotes the growth of the eel, improves the immunity, improves the eel-specific taste, improves the oily taste and reduces the fishy fish.

Meanwhile, the feed compositions for eel culturing of the present invention may further comprise 1 to 4 parts by weight of fermented extract of Areca.

If the fermented extract of Areca is further contained, the immune ability of the eel can be further improved, and the eel can be effectively removed from the fish.

The fermented extract of Areake palm is obtained by inoculating 5 to 15% (w / v) of mycelia of P. japonica on leaves of Areca wax, fermenting at 25 to 33 ° C for 10 to 15 days, adding 5 to 10 times Of ethanol or an aqueous ethanol solution is added thereto, followed by immersion extraction at 20 to 30 ° C for 5 to 7 days, followed by filtration. It is of course also possible to dry and crush it as required.

In addition, the feed composition for eel culturing of the present invention may further comprise 0.5 to 2 parts by weight of a grapefruit aged material.

The inclusion of the grapefruit maturation product further improves the taste and meat quality of the eel and decreases the fishy smell of the eel so that the sensory preference can be improved and the immunity can be increased to reduce the mortality caused by pathogenic bacteria.

Specifically, the grapefruit-aged material means that the grapefruit which has not been removed from the shell is mixed with sugar at a weight ratio of 1: 0.1 ~ 0.2, and aged at 10 ~ 25 ° C for 30 ~ 60 days and pulverized. It is of course also possible to dry and crush it if necessary. At this time, sugars and oligosaccharides can be known.

Hereinafter, the present invention will be described in detail with reference to specific examples.

(Example 1)

3 L of purified water was added to 1 kg of the pear blossom which was dried at 60 캜 and powdered to about 100 mesh, and the resultant was extracted at 60 캜 for 2 days, filtered (Whatman NO 5, whatman international Ltd, UK), concentrated under reduced pressure and lyophilized, An extract was obtained.

3kg of purified water was added to 1kg of the mudworm which had been dried at 60 ° C and powdered to about 100mesh, and the resultant was extracted at 60 ° C for 2 days, filtered (Whatman NO5, whatman international Ltd, UK), concentrated under reduced pressure and lyophilized, .

The extracts were then filtered (Whatman NO5, whatman international Ltd, UK) and concentrated under reduced pressure and lyophilized to give an extract of Safflower seed. .

(Whatman NO5, whatman international Ltd, UK), and concentrated under reduced pressure and lyophilized to obtain a berry extract of bergamot strawberry extract ≪ / RTI >

3 L of purified water was added to 1 kg of Areake palm leaf which had been dried at 60 ° C and pulverized to about 100 mesh, extracted at 60 ° C for 2 days, filtered (Whatman NO 5, whatman international Ltd, UK), concentrated under reduced pressure and lyophilized Areeka extract was obtained.

To 10 kg of the compound feed, 150 g of the above-mentioned pomegranate extract, 150 g of mugwort extract, 150 g of safflower seed extract, 150 g of bokbunja strawberry extract and 150 g of Areca palm extract were mixed. At this time, commercially available products including 100 parts by weight of fish meal, 30 parts by weight of root meal, 30 parts by weight of seaweed powder, and 5 parts by weight of fish oil were used as the compound feed.

(Example 2)

The same procedure as in Example 1 was carried out except that 70% ethanol aqueous solution was used as a solvent in the preparation of each extract.

(Example 3)

The same procedure as in Example 1 was carried out except that 150 g of the above-described extract of the oriental papaver extract, 100 g of the extract of mugwort, 100 g of safflower seed extract, 100 g of bokbunja strawberry extract, 100 g of Areca palm extract and 150 g of fermented extract of Areca palm were mixed.

The fermented extract of Areca wax was dried at 60 ° C and pulverized to about 100mesh by centrifugation at 121 ° C for 50 minutes at a temperature of 121 ° C. Then, the mycelia of P. japonica was treated with 10% (w / v) And fermented at 30 DEG C for 12 days. The fermented Areca palm leaf was prepared by adding 8 vol. Portions of ethanol, immersing and extracting at 25 ° C for 7 days, filtering (Whatman NO5, whatman international Ltd, UK) and concentrating under reduced pressure and freeze-drying.

(Example 4)

100 g of safflower seed extract, 100 g of safflower seed extract, 100 g of bokbunja strawberry extract, 100 g of Areca palm extract, 100 g of fermented extract of Areca palm and 50 g of fermented grapefruit were added to 10 kg of the compound feed, .

The grapefruit which had not been peeled was pulverized with a blender and mixed with the sugar at a weight ratio of 1: 0.2, followed by aging at 15 ° C for 40 days. The resulting grapefruit was further pulverized with a blender, And lyophilized.

(Comparative Example 1)

Only compound feed was used.

(Comparative Example 2)

400 g of the western blossom extract was mixed with 10 kg of the compound feed. At this time, the pomegranate extract was extracted in the same manner as in Example 1.

(Comparative Example 3)

To 10 kg of the compound feed, 400 g of the mugwort extract was mixed. At this time, the mugwort extract was extracted in the same manner as in Example 1.

(Comparative Example 4)

400 g of safflower seed extract was mixed with 10 kg of the compound feed. At this time, the safflower seed extract was extracted in the same manner as in Example 1.

(Comparative Example 5)

To 10 kg of compound feed, 400 g of bokbunja strawberry extract was mixed. At this time, the safflower seed extract was extracted in the same manner as in Example 1.

(Comparative Example 6)

400 g of Areca extract was mixed with 10 kg of the compound feed. At that time, the Areeka extract was extracted in the same manner as in Example 1.

(Test Example 1)

The experimental eels were classified into 10 groups, and each group was given examples and comparative examples. The eel was fed at a rate of 2 kg / m 2 per hectare in a circulation filtration tank equipped with a filtration tank and a foam separator for the experiment. The water temperature was 27 to 28 ° C For 60 days. At the beginning of the experiment, the weight per an eel was 70 to 100 g.

After 60 days, general components of each group of eels were analyzed and the results are shown in Table 1 below. General component analysis was performed according to the AOAC (2000) method.

Test Example 1 Results division Moisture (% / 100g) Ashes (% / 100g) Fat (% / 100g) Protein (% / 100g) Carbohydrate (% / 100g) 1 group
(Example 1) 70.524 1.652 6.800 16.256 4.768 2 groups
(Example 2) 70.257 1.691 6.682 16.521 4.849 3 groups
(Example 3) 70.514 1.632 6.551 16.758 4.545 4 groups
(Example 4) 70.512 1.682 6.112 17.210 4.484 5 groups
(Comparative Example 1) 66.257 1.741 10.800 15.935 5.267 6 groups
(Comparative Example 2) 66.524 1.726 9.701 15.989 6.065 7 groups
(Comparative Example 3) 66.286 1.758 9.521 16.100 6.335 8 groups
(Comparative Example 4) 66.257 1.752 9.241 16.021 6.729 9 groups
(Comparative Example 5) 66.871 1.725 9.253 15.985 6.166 10 groups
(Comparative Example 6) 66.850 1.746 9.240 15.988 6.176

As can be seen from the above Table 1, the eels prepared in Examples 1 to 4 of the present invention had a low fat content and a high protein content, thereby greatly reducing the sensation of ingestion and feeling the distinctive texture of the eel Could know. On the other hand, it was confirmed that Comparative Example 1 and Comparative Examples 2 to 6 in which the respective extracts alone were mixed with the compounded diets had no such improvement effect.

(Test Example 2)

For the sensory evaluation, the eel of each group cultured in the same manner as in Test Example 1 was prepared by baking the same time without adding any seasoning to the brazier. In the panel, 10 adults (5 males and 5 females) were selected and 10 samples were presented at a time, and the appearance, flavor, taste, texture and overall acceptability of the eel were measured by the 9 point scale method (1 point: very poor, 9 points: very good). The results were analyzed by Duncan's multiple range test after ANOVA test using SAS (Statistical Analysis System) significant difference test. The results of the sensory test are shown in Table 2 below.

Test Example 2 Results division Exterior incense flavor Texture Overall likelihood 1 group
(Example 1) 7.86 8.51 8.40 8.15 8.19 2 groups
(Example 2) 7.78 8.51 8.41 8.21 8.32 3 groups
(Example 3) 7.85 8.72 8.62 8.52 8.54 4 groups
(Example 4) 7.75 8.85 8.83 8.61 8.68 5 groups
(Comparative Example 1) 7.81 6.85 6.12 6.25 6.10 6 groups
(Comparative Example 2) 7.80 6.71 6.52 6.51 6.27 7 groups
(Comparative Example 3) 7.79 6.21 6.48 6.23 6.13 8 groups
(Comparative Example 4) 7.82 6.23 6.15 6.54 6.23 9 groups
(Comparative Example 5) 7.80 6.42 6.35 6.31 6.28 10 groups
(Comparative Example 5) 7.81 6.30 6.21 6.24 6.21

As can be seen in Table 2, there was no difference in appearance between the groups. However, it was confirmed that the eel cultured in Examples 1 to 4 of the present invention was significantly superior to the eel cultured in Comparative Examples 1 to 6 in flavor, taste, texture, and overall acceptability.

(Test Example 3)

In order to confirm the growth and mortality of the eel, the eel of each group was cultured for 30 days in the same manner as in Test Example 1. After 30 days, body weight and mortality were measured. The results are shown in Table 3 below.

Test Example 3 Results division Among the initial average group (g) After 30 days, (g) Mortality (%) 1 group
(Example 1) 84.7 141.8 4.2 2 groups
(Example 2) 83.2 140.2 4.1 3 groups
(Example 3) 84.5 143.5 3.5 4 groups
(Example 4) 85.2 145.2 3.2 5 groups
(Comparative Example 1) 82.5 131.2 19.0 6 groups
(Comparative Example 2) 84.2 134.0 18.5 7 groups
(Comparative Example 3) 83.6 132.2 19.2 8 groups
(Comparative Example 4) 84.2 133.2 19.3 9 groups
(Comparative Example 5) 83.9 131.2 18.5 10 groups
(Comparative Example 6) 83.4 132.1 17.3

As can be seen from the above Table 3, it was confirmed that the eel fed with Examples 1 to 4 of the present invention was remarkably higher in growth rate than the eel fed with Comparative Examples 1 to 6, and the mortality was greatly suppressed.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation.

Claims (4)

1 to 4 parts by weight of extracts of Pyrus communis flower, 1 to 4 parts by weight of extracts of mugwort, 1 to 4 parts by weight of safflower seed extract, 1 to 4 parts by weight of bokromatic strawberry extract and 1 to 4 parts by weight of Areeka extract, And 4 parts by weight of the composition.
The method according to claim 1,
The western blossom extract is obtained by extracting western blossom with water, ethanol or a mixture thereof,
The extract of the mugwort extract is obtained by extracting mugwort with water, ethanol, or a mixture thereof,
The safflower seed extract is obtained by extracting the seeds of safflower with water, ethanol, or a mixture thereof,
The bramble strawberry extract is obtained by extracting the fruit of bramble strawberry with water, ethanol, or a mixture thereof, as a solvent,
Wherein the Areeka extract is obtained by extracting Areeka leaves with water, ethanol, or a mixture thereof, as a solvent.
3. The method of claim 2,
1 to 4 parts by weight of a fermented extract of Areeka,
The fermented extract of Areeka,
Wherein the composition is fermented and extracted by inoculating a mycelium of myrrh into a leaf of Areeka.
The method according to claim 2 or 3,
0.5 to 2 parts by weight of a grapefruit-ripened product,
Wherein the grapefruit aged material is prepared by mixing sugar with grapefruit at a weight ratio of 1: 0.1 ~ 0.2 and then aging at 10 ~ 25 ° C for 30 ~ 60 days.