KR102293593B1 - Skin external composition comprising Asparagopsis taxiformis extract and functional food comprising extract of Asparagopsis taxiformis extract - Google Patents

Abstract

The present invention relates to a skin external composition and functional food comprising an Asparagopsis taxiformis extract as an active ingredient. It is an object of the present invention to provide the skin external composition and functional food composition for improving skin barrier functions.

Description

Skin external composition comprising Asparagopsis taxiformis extract and functional food comprising extract of Asparagopsis taxiformis extract

The present invention relates to a composition for external application to the skin and a functional food containing a seaweed extract as an active ingredient.

The skin barrier is the outermost stratum corneum of the skin and can be said to be the first barrier layer of the skin. The skin barrier is composed of keratinocytes, keratinocytes, lipids such as ceramides, and proteins such as filaggrin, which form a multi-layered structure, thereby serving as an intact skin barrier. The primary function of the skin barrier is to prevent the loss of body fluids, the attack of toxins, and the entry of pathogens. Therefore, damage to the skin barrier can cause serious immune responses through penetration of various pathogens, not just mechanical damage to the outer layer of the skin.

The limitation of antibiotic therapy, which is one of the existing treatment methods, was that it suppressed not only harmful skin bacteria (ex. Staphylococcus aureus) but also beneficial skin bacteria (ex. Staphylococcus epidermidis), so the effect was not clear. Therefore, it is necessary to develop a material that improves the skin barrier function by suppressing only harmful bacteria without inhibiting skin symbiotic beneficial bacteria.

The microbiome is a compound word of microbiota, which refers to the microbial community, and genome, which refers to the genome. Microbiota is a term that focuses on the microorganism strain itself compared to the microbiome, and refers to microorganisms that inhabit the human body and have a symbiotic relationship. It refers to research and technology based on genomics of interactions between humans. It is well known that maintaining the balance of the microbiome affects the functioning of the human body. Recently, many studies have been conducted on the skin microbiome and the skin barrier in the fields of cosmetics, medicine, and pharmaceuticals. Accordingly, cosmetics and external preparations for skin are being actively developed.

An object of the present invention is to provide a composition for external application to the skin and a functional food composition for improving the skin barrier function.

An aspect of the present invention for achieving the above object is,

It is to provide a composition for external application to the skin comprising a seaweed extract as an active ingredient.

According to one embodiment, the composition is preferable for preventing damage to the skin barrier.

According to one embodiment, it is preferable that the seaweed extract is included in an amount of 0.001 to 10% by weight based on the total weight of the composition.

According to one embodiment, the seaweed extract can improve the skin barrier function by inhibiting the biofilm formation of harmful bacteria.

According to one embodiment, the harmful bacteria is Staphylococcus aureus.

According to one embodiment, the seaweed extract can improve the skin barrier function by maintaining the skin microbiome balance.

According to one embodiment, it is preferable that the seaweed extract is extracted with water, an organic solvent, or a mixture thereof.

According to one embodiment, the organic solvent is preferably at least one selected from anhydrous or containing alcohols having 1 to 4 carbon atoms, glycerin, propylene glycol and butylene glycol, ethyl acetate, hexane and chloroform.

According to one embodiment, the composition for external application for skin is one or more formulations selected from the group consisting of lotions, gels, water-soluble liquids, creams, powders, oil-in-water (O/W) types and oil-water (W/O) types. desirable.

According to one embodiment, it is preferable that the composition for external application for skin is a cosmetic composition.

According to one embodiment, it is preferable that the composition for external application for skin is a quasi-drug composition for improving skin barrier function.

In order to achieve another object of the present invention, it provides a health functional food composition for improving skin barrier function, comprising an Asparagopsis taxiformis extract as an active ingredient.

In the present invention, the composition for external application to the skin containing the seaweed extract is effective in preventing damage to the skin barrier by preventing damage to skin cells and maintaining the balance of the skin microbiome.

In particular, since it is effective in inhibiting the biofilm formation of Staphylococcus aureus, it can greatly prevent skin damage caused by Staphylococcus aureus in a state in which the skin barrier is damaged, such as the skin of atopic patients among various diseases, It is very effective for patients with the same skin disease.

In addition, since the cosmetic composition contains a natural material as an active ingredient, there is little side effect or risk due to a synthetic material.

1 is a photograph showing the biofilm formation inhibitory ability of the seaweed extract.
Figure 2 is a graph showing the biofilm formation inhibitory ability of seaweed extract.
Figure 3 is a graph showing the degree of inhibition of the hemolytic activity of Staphylococcus aureus by the extract of seaweed.

Hereinafter, the present invention will be described in more detail. However, this is only specifically described to help the understanding of the present invention, and the scope of the present invention is interpreted based on the claims.

The present invention relates to a composition for external application for skin comprising an extract of seaweed as an active ingredient.

Asparagopsis taxiformis is a species belonging to the red algae and is distributed worldwide in tropical and temperate waters.

In one embodiment of the present invention, the seaweed extract may be included in an amount of 0.001 to 10.0% by weight, preferably 0.01 to 0.2% by weight, based on the total weight of the composition.

If the content is less than 0.001% by weight, an effective effect cannot be exhibited, and if it exceeds 10.0% by weight, there is a problem in formulation stability such as discoloration and odor.

According to one embodiment, the seaweed extract can improve the skin barrier function by inhibiting the biofilm formation of harmful bacteria. Among them, it is possible to alleviate the toxicity by inhibiting the biofilm formation of Staphylococcus aureus, thereby improving the skin barrier function.

Biofilm is also called biofilm or biofilm as a microbial complex wrapped in various polymer materials formed by microorganisms. This biofilm acts as a protective film that helps microorganisms adapt to various environments, and the cells in the biofilm share metabolism with each other and acquire properties favorable for survival through gene transfer. . Therefore, the cells that form the biofilm have much stronger resistance to harsh environments and antibiotics than when they exist in the state of general airborne bacteria, and the biofilm formed in the organ causes various diseases. Therefore, by suppressing the biofilm formation of harmful bacteria, the toxicity of harmful bacteria can be alleviated.

Staphylococcus aureus is present on the skin surface and pores of the human body. In general, Staphylococcus aureus, which resides on the skin, does not cause disease in normal people, but Staphylococcus aureus causes disease in normal people. In particular, it often develops when it enters the body through a wound. The virulence factor of Staphylococcus aureus is largely divided into virulence factors present in cells, external toxins, and enzymes. As toxic factors present in cells, protein A, fibronectin-binding factor, and teichoic acid are included. External toxins include enterotoxin, toxic shock syndrome toxin-1 (TSST-1), and α-hemolysin (Hla), and enzymes are coagulase, staphylokinase, and protease. ), DNase, and lipase (Encyclopedia of Microbiology). Staphylococcus aureus with these characteristics acts as a causative agent that causes various epidermal infections such as abscesses, food poisoning, pneumonia, meningitis, and sepsis. Among various diseases, in a state where the skin barrier is damaged, such as the skin of atopic patients, Staphylococcus aureus forms colonies and biofilms well, thereby exacerbating symptoms due to inflammatory response and damage to skin cells. it is well known that As a result, the distribution of skin microbes is different between those with a normal skin barrier and those without.

The present inventors have found that the seaweed extract is effective in improving the skin barrier function, particularly in inhibiting Staphylococcus aureus. Therefore, the composition according to the present invention can improve and maintain the skin barrier function more effectively for damaged or weakened skin, especially for skin diseases, and has the effect of strengthening skin moisturizing power, preventing skin troubles and preventing skin aging .

In addition, according to one embodiment, the seaweed extract can improve the skin barrier function by maintaining the skin microbiome balance.

In the present invention, the seaweed extract may be extracted by a conventional extraction method well known in the art using water, an organic solvent, or a mixed solvent thereof, and extraction using an organic solvent is preferable.

The organic solvent may be at least one selected from the group consisting of anhydrous or containing alcohols having 1 to 4 carbon atoms, glycerin, propylene glycol, butylene glycol, ethyl acetate, hexane and chloroform.

In one embodiment of the present invention, as the most preferred extract, the ethanol extract of seaweed may be used.

For the extract, methods such as cold-chim extraction, warm-chim extraction, or heat extraction may be used, and a conventional extraction device, ultrasonic pulverization extractor, or fractionator may be used.

In one embodiment of the present invention, the prepared extract may be filtered, concentrated, or dried to remove the solvent, specifically, the filtration may use a filter paper or a reduced pressure filter, and the concentration may be a reduced pressure concentrator, one For example, it may be concentrated under reduced pressure using a rotary evaporator, and the drying may be performed, for example, by freeze-drying.

In the present invention, the composition may further include various components according to the method of use and the purpose of use in addition to the seaweed extract.

According to an embodiment of the present invention, the composition may be prepared and used in one or more formulations selected from the group consisting of skin, lotion, cream and powder, but is not limited thereto.

Specifically, it is preferable that the composition for external application for skin is at least one formulation selected from the group consisting of a lotion, a gel, a water-soluble liquid, a cream, a powder, an oil-in-water (O/W) type, and a water-based (W/O) type.

According to one embodiment, it is preferable that the composition for external application for skin is prepared as a cosmetic composition. Even with daily use, it is composed of natural substances, and it continuously improves the skin barrier function without skin troubles, thereby providing effects such as skin moisturizing, wrinkle improvement and anti-aging. The cosmetic composition according to an embodiment of the present invention is not limited to being prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, It may be formulated as powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it can be applied to the manufacture of functional cosmetics, which are basic cosmetics, such as a lotion, a nourishing cream, a moisture cream, an essence, an eye cream, a lotion, a pack, and an essence. On the other hand, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the fermentation extract as an active ingredient, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. customary adjuvants, and carriers.

Also, according to one embodiment, the present invention can be used as a quasi-drug composition for improving skin barrier function. Like the above-described cosmetic composition, the quasi-drug composition may also have one or more uses selected from the group consisting of skin moisturizing enhancement, skin trouble prevention, and skin aging prevention.

The term "quasi-drug" used in the present invention refers to articles with a milder action than pharmaceuticals among articles used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals, for example, the Pharmaceutical Affairs Act. According to the Act, quasi-drugs exclude products used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans or animals, and products that do not act directly on the human body, etc.

The quasi-drug composition is used for the purpose of improving the skin barrier function, more specifically, for the purpose of strengthening skin moisture, preventing skin troubles and preventing skin aging, and there is no particular limitation in the formulation thereof, for example, softening lotion, nutrition It may be a composition having the formulation of a lotion, massage cream, nourishing cream, pack, mask pack, mask sheet, gel, or skin adhesion type cosmetic, and also a transdermal formulation such as a lotion, ointment, gel, cream, patch or spray. It may be a composition having

On the other hand, the quasi-drug composition may include, for example, thickeners, stabilizers, solubilizers, vitamins, conventional adjuvants such as pigments and fragrances, and carriers in addition to the above extracts as necessary by additionally blending them.

Meanwhile, according to an embodiment of the present invention, there is provided a health functional food composition for improving skin barrier function containing an Asparagopsis taxiformis extract as an active ingredient. Like the cosmetic composition described above, the health functional food composition may also have one or more uses selected from the group consisting of skin moisturizing enhancement, skin trouble prevention, and skin aging prevention.

In the health functional food composition according to an embodiment of the present invention, there is no particular limitation on the type of health functional food, for example, meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, gum, It may be dairy products, various soups, beverages, teas, drinks, alcoholic beverages, vitamin complexes, etc., and may include all foods in a conventional sense.

On the other hand, the food composition may include ingredients commonly used in health functional food compositions in addition to the extract, for example, nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonates used in carbonated beverages, or combinations thereof.

Hereinafter, in order to describe the present invention in detail, it will be described in detail with reference to Experimental Examples and Examples. However, the experimental examples according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the experimental examples and examples described in detail below. Experimental examples and examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Preparation Example 1: Preparation of seaweed extract

The dried seaweed outpost was pulverized and immersed in 95% ethanol at a weight ratio of twice the weight, and exposed to an ultrasonic device (150 w, 30° C.) for 30 minutes. After immersion for 24 hours, it was filtered through filter paper and the solvent was removed with a rotary vacuum concentrator to prepare an extract.

Experimental Example 1: Strain, cell line and culture conditions

The strain was used in the experiment by culturing Staphylococcus aureus (S. aureus, ATCC 6538), Tryptic soy broth (TSB) at 37°C temperature condition as a skin harmful bacteria.

Experimental Example 2: Measurement of inhibition of biofilm formation

The biofilm formation inhibitory ability of the seaweed extract was confirmed through the crystal violet staining method.

Before proceeding with the biofilm formation inhibitory ability test of Staphylococcus aureus extract by the extract, it was confirmed that the minimum growth inhibitory concentration (MIC) of the Staphylococcus aureus extract was 2 mg/ml or more through a preliminary experiment. .

^{About 2-5x10 5} CFU/well of Staphylococcus aureus, 200, 100, 50, 25, 12.5 μg/ml of each Staphylococcus aureus extract, 12.5 μg/ml of each 96-well microplate, and 200 μl of a liquid medium containing 1% glucose were treated. The 96-well microplate was incubated at 37° C. for 24 hours.

After 24 hours of incubation, the medium was removed, and 200 μl of purified water was added to each well to remove non-attached floating bacteria and bacteria weakly bound to the biofilm, repeated three times. Then, 200 μl of 0.1% crystal violet staining reagent was added to each well and reacted at room temperature for 15 minutes. After 15 minutes, the wells were washed 3 times with purified water to remove the residual dye, and the wells were photographed and shown in FIG. 1, dissolved in 95% ethanol and absorbance was measured at 540 nm using a microplate reader. The amount of biofilm formation in the experimental group treated with the extract of seaweed extract compared to the untreated control group was shown in a graph as shown in FIG. 2 .

As shown in Figures 1 and 2, it was confirmed that the biofilm formation of Staphylococcus aureus was inhibited at a concentration of 25㎍ / ㎖ or more of seaweed of the present invention.

Experimental Example 2: Measurement of hemolytic activity inhibition

In order to measure the inhibition of hemolytic activity of Staphylococcus aureus by the extract of seaweed, a hemolysis assay using rabbit blood was performed.

Before proceeding with experiments related to the toxicity of Staphylococcus aureus by the extract, it was confirmed that the minimum growth inhibitory concentration (MIC) of the extract of Staphylococcus aureus against Staphylococcus aureus was 2 mg/ml or more through a preliminary experiment.

Staphylococcus aureus 1x10 ⁶ CFU/ml of seaweed extract was treated with 100, 50, and 25 μg/ml and cultured in TSB medium for 24 hours. The cultured bacterial solution was centrifuged to obtain only the supernatant, filtered through a 0.2 μm syringe filter, and then used in the experiment.

25 μl of rabbit blood washed 3 times in PBS and 975 μl of the bacterial culture supernatant were placed in a 1.5 ml microtube, incubated at 37° C. for 1 hour, and centrifuged at 5,500 g (gravity acceleration) for 1 minute to obtain 200 μl of the supernatant. After transferring to a microplate, absorbance was measured at 540 nm using a microplate reader.

As shown in FIG. 3 , it was confirmed that the hemolytic activity by the Staphylococcus aureus culture medium was inhibited in the experimental group treated with the seaweed extract of the present invention.

According to the experimental results, it was confirmed that the seaweed extract according to the present invention is a natural material having an effect of preventing damage to the skin barrier by suppressing the biofilm formation of Staphylococcus aureus to alleviate toxicity.

Hereinafter, on the basis of the results of the above experimental examples, various formulation examples using the seaweed extract are formulated and presented. However, these formulation examples are for explaining the present invention, and it is obvious to those of ordinary skill in the art that the formulations of the present invention are not limited to these formulation examples.

Formulation Examples 1 to 3: Preparation of a cosmetic composition containing a seaweed extract

Formulation Example 1 Lotion (emulsion)

Ingredient name Example (wt%) stearic acid 0.5 ethanol 10.0 vaseline 1.0 squalane 2.5 liquid paraffin 6.0 Monooleate polyoxyethylene sorbitan 1.5 1,3-butylene glycol 8.0 triethanolamine 0.05 Spices 0.2 antiseptic appropriate amount antioxidant appropriate amount seaweed extract 0.05 Purified water To 100

Formulation Example 2: Softening lotion (skin toner)

Ingredient name weight% glycerin 5.0 1,3-butylene glycol 3.0 ethanol 5.0 Polyoxyethylene nonylphenyl ether 0.5 Spices appropriate amount antiseptic appropriate amount seaweed extract 0.05 Purified water To 100

Formulation Example 3: Cream

Ingredient name Example (wt%) glycerin 5.0 stearic acid 8.0 squalane 5.0 Self-emulsifying glycerin monostearate 2.5 Monostearate polyoxyethylene sorbitan 1.5 propylene glycol 4.0 stearylglycyretinate 0.2 vaseline 2.0 antioxidant appropriate amount Spices dose antiseptic appropriate amount seaweed extract 0.05 Purified water To 100

Claims (11)

A composition for external application for skin comprising an Asparagopsis taxiformis extract as an active ingredient. According to claim 1,
The composition is a composition for external application to the skin for preventing damage to the skin barrier. According to claim 1,
The composition for external application for skin, characterized in that the sea cockle extract is included in an amount of 0.001 to 10% by weight based on the total weight of the composition. According to claim 1,
The seaweed extract is an external composition for skin, characterized in that it inhibits the biofilm formation of Staphylococcus aureus. According to claim 1,
The sea cockle extract is a composition for external application for skin, characterized in that it maintains the skin microbiome balance. According to claim 1,
The composition for topical application for skin, characterized in that the sea cockle extract is extracted with water, an organic solvent, or a mixture thereof. 7. The method of claim 6,
The composition for external application for skin, characterized in that the organic solvent is at least one selected from anhydrous or containing alcohols having 1 to 4 carbon atoms, glycerin, propylene glycol, butylene glycol ethyl acetate, hexane and chloroform. According to claim 1,
The composition for external application for skin is prepared in a formulation selected from the group consisting of a lotion, a gel, a water-soluble liquid, a cream, a powder, an oil vapor (O/W) type, and a water vapor (W/O) type. 9. The method according to any one of claims 1 to 8,
The composition for external application for skin is a cosmetic composition. 9. The method according to any one of claims 1 to 8,
The composition for external application for skin is a quasi-drug composition for improving skin barrier function, composition for external application for skin. A health functional food composition for improving skin barrier function, comprising an Asparagopsis taxiformis extract as an active ingredient.

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