KR101768613B1 - Composition for antimicrobial comprising codium fragile extract - Google Patents

Abstract

The present invention relates to an antimicrobial composition comprising a Codium fragile extract. The Codium fragile extract has excellent antimicrobial activity against various pathogenic microorganisms and antibiotic resistant microorganisms, thereby being able to be used as an antimicrobial composition, a pharmaceutical composition, a food composition and a cosmetic composition.

Description

TECHNICAL FIELD [0001] The present invention relates to an antimicrobial composition containing an auditory extract as an active ingredient.

The present invention relates to an antimicrobial composition containing an auditory extract as an active ingredient.

Antibiotics are generally referred to as antimicrobial agents in general, and in particular, substances which have antimicrobial activity against bacteria, in particular, agents that inhibit systems for synthesizing cell walls and proteins, it means. The most common antibiotic is penicillin manufactured by the British physician Alexander Fleming in 1928, and methicillin, which is considered to be superior to penicillin, is a representative antibiotic developed after penicillin. Methicillin was made by partially modifying the chemical structure of penicillin.

Antibiotic-resistant bacteria refer to bacteria that are resistant to certain antibiotics and that do not respond to antibiotics. For example, there is penicillin-resistant Staphylococcus aureus , Methicillin-Resistant Staphylococcus aureus (MRSA), which has no effect on the efficacy of penicillin. MRSA has been found to have a unique gene that is resistant to antibiotics such as penicillin or methicillin. MRSA is not infectious to healthy people, and has been reported to cause sepsis or pneumonia, resulting in death due to infections in patients with poor immunity or surgery.

Due to the emergence of these antibiotic resistant bacteria, we have been interested in the development of new antimicrobial agents using various plant extracts that go beyond conventional antibiotics. Especially, the antimicrobial agent obtained from the extract of such plants is less toxic than the synthetic antimicrobial agent composed of the compound, and has an advantage that it is safe to human body because it is not corrosive. Korean Patent Registration No. 10-0852744 discloses that an extract of a dragonfly has antimicrobial activity against methicillin-resistant Staphylococcus aureus.

1. Korean Patent No. 10-0852744

One aspect of the present invention is to provide an antibacterial composition comprising a Codium fragile extract as an active ingredient.

Another aspect of the present invention is to provide a pharmaceutical composition, a food composition and a cosmetic composition comprising the antimicrobial composition.

One aspect of the present invention provides an antimicrobial composition comprising an auditory extract as an active ingredient.

Hearing ( Codium fragile ) is widely distributed throughout the world as a seaweed of the green algae plant, and it is widely used in the Philippines, Hawaii, and other countries as well as Korea, China and Japan. It is rich in calcium, potassium and iron content because it contains many minerals than land plants.

According to one embodiment of the present invention, the auditory extract may be prepared according to a conventional method known in the art. Specifically, after the hearing is washed, dried and pulverized, the auditory extract can be prepared using a conventional solvent under the conditions of ordinary temperature and pressure.

The solvent may be selected from the group consisting of distilled water, n-hexane, 1,3-butylene glycol, alcohols having 1 to 4 carbon atoms, ethyl acetate, chloroform, diethyl ether, dichloromethane, hexane, acetone, And is preferably distilled water or an alcohol having 1 to 4 carbon atoms.

Meanwhile, the auditory extract includes not only the extract obtained by the above-mentioned solvent extraction method but also the extract obtained through a conventional purification process. For example, a fraction obtained through various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) May also be included in the peel extract of the present invention. In addition, the auditory extract may be prepared in powder form by an additional process such as vacuum distillation, freeze-drying, or spray drying. Specifically, the hearing extract may be prepared in powder form through sterilization and spray drying, sterilized at a temperature of 70 to 90 ° C. for 10 to 40 minutes, and then powdered using a spray dryer .

In the present invention, the antimicrobial composition may have the same meaning as an antibiotic, which is generically referred to as an antimicrobial agent, and may have the same meaning as an antimicrobial agent, a bactericide, an antiseptic, a preservative or a bactericidal agent, Means a substance capable of inhibiting or inhibiting development and function of life.

The antimicrobial composition of the present invention may contain 0.001% by weight to 99.99% by weight, preferably 0.1% by weight to 99% by weight of the above-described hearing extract, based on the total weight of the composition. Accordingly, the content of the active ingredient can be appropriately controlled.

According to one embodiment of the present invention, the antimicrobial composition comprises at least one member selected from the group consisting of genus Staphylococcus , genus Salmonella , genus Enterococcus , genus Escherichia , genus Bacillus it is preferable that the microorganism has an antibacterial activity against a microorganism selected from the group consisting of genus Bacillus , genus Klebsiella , genus Micrococcus , genus Proteus and genus Pseudomonas . More preferably, Staphylococcus aureus , Salmonella enterica , Enterococcus faecalis , Escherichia coli, coli), Bacillus subtilis (Bacillus subtilis), keulrep when Ella pneumoniae (Klebsiella pneumoniae , Micrococcus luteus , Proteus hauseri and Pseudomonas aeruginosa , and the like.

In addition, the Staphylococcus microorganism may be selected from the group consisting of methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) Is preferably selected.

The term " methicillin-resistant Staphylococcus aureus " in the present specification is also called methicillin-resistant Staphylococcus aureus, and refers to a bacterium having a strong resistance to almost all antibiotics such as penicillin and methicillin. It is a major cause of hospital infection. It is found in a large hospital that uses many antibiotics. It penetrates into the respiratory system and the affected part of the operation patient and causes serious symptoms such as high fever, chills, pneumonia and blood pressure decrease.

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating diseases comprising the above-mentioned antimicrobial composition.

According to one embodiment of the invention, the disease is selected from the group consisting of food poisoning, infectious disease, gastroenteritis, urinary tract infections, sepsis, pneumonia, meningitis and folliculitis follicultis).

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier. The term "pharmaceutically acceptable" as used herein refers to a composition that is physiologically acceptable and does not normally cause an allergic reaction such as gastrointestinal disorder, dizziness, or the like when administered to humans. Pharmaceutically acceptable carriers include, for example, carriers for oral administration such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid and the like, water for parenteral administration such as water, suitable oils, saline, aqueous glucose and glycols And may further contain stabilizers and preservatives. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives include benzalkonium chloride, methyl- or propyl-paraben and chlorobutanol. Other pharmaceutically acceptable carriers can be found in Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, Pa., 1995). The pharmaceutical composition according to the present invention may be formulated into a suitable form according to a method known in the art together with a pharmaceutically acceptable carrier as described above. That is, the pharmaceutical composition of the present invention can be prepared in various parenteral or oral administration forms according to known methods. Representative examples of formulations for parenteral administration include isotonic aqueous solutions or suspensions in the form of injection. The injectable formulations may be prepared according to techniques known in the art using suitable dispersing or wetting agents and suspending agents. For example, each component may be formulated for injection by dissolving in saline or buffer. In addition, formulations for oral administration include, but are not limited to, powders, granules, tablets, pills, and capsules.

The pharmaceutical composition formulated as described above may be administered in an effective amount through various routes including oral, transdermal, subcutaneous, intravenous, or muscular. The term " effective amount " as used herein refers to an amount that shows a preventive or therapeutic effect when administered to a patient. The dosage of the pharmaceutical composition according to the present invention can be appropriately selected depending on the route of administration, subject to be administered, age, gender, individual difference, and disease state.

Yet another aspect of the present invention provides a food composition comprising said antimicrobial composition.

The food composition may be added to foods, beverages, or the like to lower the possibility of infection of a subject having a high risk of infection by a pathogenic microorganism, or may be used as a raw material for foods and beverages. Examples of foods containing the food composition include various foods, beverages, gums, tea, vitamin complexes, health supplements, and the like, and they can be used as powder, granule, tablet, capsule or beverage. The food composition of the present invention can generally be added at 0.01 to 15% by weight of the total food, and the health beverage composition can be added at a ratio of 0.02 to 10 g, preferably 0.3 to 1 g, based on 100 ml. The food composition of the present invention can contain, besides containing the food composition as an essential ingredient in the indicated ratios, a food-acceptable food-aid additive such as natural carbohydrates and various flavoring agents as additional ingredients have.

Further, the food composition can be added to impart preservability to the food to be used. Since the antimicrobial composition is a natural ingredient, it is not harmful to the human body and can be safely applied to food.

The food composition may further include other additives appropriately selected according to the purpose and use of the food, and it may be mixed with other active ingredients such as additives used in common use, additives such as coloring matters, surfactants, preservatives, etc. . The additive may be prepared by pulverizing, granulating, tableting or liquefying according to the purpose and use of the additive. For commercialization, a conventional method may be used for packaging.

The amount of the antimicrobial composition to be used in the food composition may be suitably selected depending on the purpose of use, the application form, the application site, the object to be applied, and the like. For example, the antimicrobial composition may contain 0.01 to 50 parts by weight, The content of the antimicrobial composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

Another aspect of the present invention provides a cosmetic composition comprising the antimicrobial composition.

The cosmetic composition may be added in order to impart preservability to the cosmetics used, and the antimicrobial composition containing the auditory extract is a natural ingredient and is not harmful to the human body, so that it can be safely applied to cosmetics.

The cosmetic composition of the present invention may contain, in addition to the auditory extract as an active ingredient, components commonly used in cosmetic compositions and may contain conventional additives such as stabilizers, solubilizers, vitamins, pigments and fragrances, have.

Meanwhile, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, Containing cleansing oil, powdered foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto. More particularly, the present invention relates to a cosmetic composition for the preparation of a cosmetic composition, which comprises at least one component selected from the group consisting of a soft lotion, a gel, a water-soluble liquid, a milk lotion, a cream, an essence, a skin toner, an oil-in-water emulsion, , Pack, body oil, body lotion, underwater type make-up base, in-water type make-up base and foundation.

The auditory extract of the present invention has excellent antimicrobial activity against various pathogenic microorganisms and antibiotic resistant microorganisms, and thus can be used for antimicrobial compositions, pharmaceutical compositions, food compositions and cosmetic compositions.

FIG. 1 is a flowchart illustrating a process for preparing an extract of an alcoholic beverage according to an embodiment of the present invention.
FIG. 2 is a flowchart illustrating a process for producing an auditory hot-water extract according to an embodiment of the present invention.
FIG. 3 is a graph showing antimicrobial activity of the extract of aqua extract and hot water extract against Staphylococcus aureus .
FIG. 4 is a graph showing the antibacterial activity of the extract of the alcoholic beverage and the extract of the hot water for S. aureus CCARM 0027 strain.
5 is a graph showing the antimicrobial activity of the extract of AHA and hot water extract against S. aureus CCARM 0204 strain.
FIG. 6 is a graph showing the antibacterial activity of the extract of AHA and hot water extract against S. aureus CCARM 0205 strain.
FIG. 7 is a graph showing the antibacterial activity of the extract of AHA and hot water extract against Salmonella enterica ATCC 14028 strain.
8 is Enterococcus faecalis (Enterococcus faecalis ) 19433 strain of the present invention.
Figure 9 is a graphical representation of the expression levels of Escherichia coli coli ) ATCC 25922 strain of the present invention.
10 is a graph showing antimicrobial activity of the extract of AHA and hot water extract against Bacillus subtilis ATCC 6633 strain.
11 is ammonia (Klebsiella keulrep when Ella New pneumoniae ATCC 10031 strains of the present invention.
FIG. 12 is a graph showing the antibacterial activity of the extracts of auditory and hot water for Micrococcus luteus .
13 is Proteus Howe serie (Proteus hauseri ) NBRC 3851 strain according to the present invention.
Fig. 14 is a graph showing Pseudomonas < RTI ID = 0.0 > aeruginosa ) and an antimicrobial activity of hot water extract.
FIG. 15 is a graph showing antimicrobial activity of the extract of the alcoholic beverage and the extract of the hot water for the methicillin-resistant S. aureus CCARM 3089 strain.
FIG. 16 is a graph showing antimicrobial activity of the extract of the alcoholic beverage and the extract of hot water for the methicillin resistant S. aureus CCARM 3090 strain. FIG.
FIG. 17 is a graph showing the antibacterial activity of the extract of the extract of the sea water and the extract of the hot water for the methicillin-resistant S. aureus CCARM 3634 strain.
18 is a graph showing antimicrobial activity of the extracts of auditory and hot water for the methicillin resistant S. aureus CCARM 3635 strain.
FIG. 19 is a graph showing antimicrobial activity of the extract of AHA and hot water extract against methicillin resistance S. aureus CCARM 3640 strain.
FIG. 20 is a graph showing antimicrobial activity of the extract of the alcohol extract and the extract of the hot water for the methicillin resistant S. aureus ATCC 33591 strain. FIG.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the present invention is not limited to these embodiments.

Example  1: Manufacture of auditory powder

The hearing was purchased from Hanyang Farm (Naju, Korea), and the adherents were removed, washed with clean fresh water, and completely dried at room temperature. 10 kg of dry hearing was added to the extraction tank, and 50% of the alcohol was added at a weight of 8 to 15 times the weight of the hearing, and extracted at 70 캜 for 4 to 10 hours. Next, 50% alcohol was further added at a weight of 6 to 10 times the hearing weight and extracted at 70 DEG C for 2 to 8 hours. After the extraction was completed, the alcohol extract was recovered, and the extract was filtered using a 1.0 탆 filter (Rainbow, Korea), and the filtrate was concentrated by vacuum concentration. The extracts of the auditory extracts were sterilized at 80 to 85 for 30 minutes and filtered through inlet 170, outlet 85 ° C and 10-100% SD (spray dried, 10-100% solids) using a spray dryer (Mihyun Engineering Co., ). ≪ / RTI > After extracting the extracted extract, the extracts were examined for the presence or absence of the constituents, foreign matter, moisture, common bacteria and E. coli.

In addition, purified water was added at a weight of 8 to 15 times the weight of the hearing aid instead of the alcohol, extracted at 120 to 6 to 12 hours, and further added with 6 to 10 times the weight of purified water to extract at 120 to 6 to 12 hours. The hot-water extract was filtered, concentrated and dried in the same manner as above to recover the extracted hot-water hot water. The recovered powders were inspected for the same items as above and confirmed to be safe.

FIG. 1 and FIG. 2 are flowcharts sequentially illustrating the method for producing an auditory extract of the present invention.

Experimental Example  1: Identification of hearing antimicrobial activity against microorganisms

1-1: Test strains and culture medium

Microorganisms used in the experiment are antibiotic-resistant strain banks; receive pre-sale to (Culture Collection of Antimicrobial Resistant Microbes, CCARM Seoul Women's University, the first Hall of Science, Republic of Korea) antibiotic susceptible microorganisms (methicillin-sensitive Staphylococcus aureus, MSSA ) or from the ATCC (Ameriacan Type Culture Collection, VA, USA). The strains with CCARM No. were purchased from the antibiotic-resistant strains bank, and the microbial culture medium was Mueller Hinton broth (MHB, Sigma-Aldrich, USA).

Table 1 summarizes the microorganisms used for measuring the antimicrobial activity of auditory extracts.

1-2: Measurement of antimicrobial activity

The antimicrobial activity of the hearing extract against the strains shown in Table 1 was confirmed by measuring the minimun inhibitory concentration (MIC).

Specifically, 158 [mu] l of MHB medium and 80 [mu] l of the remaining vertical lines were dispensed in the first vertical line of a 96-well plate, and the samples to be examined (auditory hot water extract, auditory alcohol extract, ampicillin and DMSO ) Was added to each well and serial dilution was performed. Next, 20 μl ( ⁵ × 10 ⁵ cfu / ml) of the strains shown in Table 1 were inoculated into each well and cultured in a 37 ° C. incubator for 16 to 20 hours. After incubation, the IC ₅₀ value was measured using an absorbance microplate reader.

As a result, as shown in Figs. 3 to 14, it was found that the antimicrobial activity of the auditory extract showed better antimicrobial activity at a similar or low concentration of ampicillin, and particularly, the antimicrobial activity of the extract of the alcohol extract was remarkably excellent .

3 to 14 are graphs showing the effect of inhibiting the growth of auditory extracts on the microorganisms listed in Table 1. The vertical axis of the graph indicates the degree of growth of microorganisms and the lower the value, the more inhibited the growth of microorganisms do.

In addition, as shown in Table 2 below, MIC of audible extracts of each strain was confirmed, and it was found that the extract of audible spirits had lower antioxidative activity than that of auditory hot water extract.

Experimental Example  2: Identification of hearing antimicrobial activity against antibiotic resistant microorganisms

The antibiotic-resistant strains used in the experiments were either purchased from the ATCC or purchased from methicillin-resistant Staphylococcus aureus ( MRSA) at the national antibiotic-resistant strain bank.

The culture medium used was the same as in Experimental Example 1-1, and the antibiotic-resistant strains used in the experiment were summarized in Table 3 below.

The antibacterial activity was the same as in Experimental Example 1-2, and the results are shown in FIGS. 15 to 20.

15 to 20 are graphs showing the effect of inhibiting the growth of auditory extracts on the antibiotic-resistant microorganisms listed in Table 3 above.

In addition, the MIC of the antibiotic resistant microorganism was measured, and it was confirmed that the antitumor activity of the extract of the alcohol extract was remarkably excellent.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

An antimicrobial composition for a microorganism of the genus Staphylococcus containing Codium fragile hydrothermal or 50% alcohol extract as an active ingredient.
delete delete The method according to claim 1, wherein the Staphylococcus microorganism is selected from the group consisting of methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus ≪ / RTI >
Comprising the antimicrobial composition of claim 1 for the treatment or prevention of food poisoning, infectious disease, gastroenteritis, urinary tract infections, sepsis, pneumonia, meningitis and follicultis. A pharmaceutical composition for the prevention or treatment of diseases selected from the group.
A food composition comprising the antimicrobial composition of claim 1.
A cosmetic composition comprising the antimicrobial composition of claim 1.

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