KR102099788B1 - Anti-oxidative and Anti-inflammatory Composition of Starfish Extract from Crossaster papposus japonicus and Preparation Method Thereof - Google Patents

Abstract

The present invention provides an anti-oxidant and anti-inflammatory composition and a method for preparing the same, which includes excellent extracts of Wax Starfish ( Crossaster papposus japonicus ) as an active ingredient, and a food composition, cosmetic composition, or the like thereof. It can be used for pharmaceutical composition.

Description

Anti-oxidative and Anti-inflammatory Composition of Starfish Extract from Crossaster papposus japonicus and Preparation Method Thereof}

The present invention relates to a composition for anti-oxidation and anti-inflammatory, and a method of manufacturing the same as the active ingredient containing the extract of Dwarf starfish ( Crossaster papposus japonicus ).

Unlike the West Sea or the South Sea, Korea's East Sea has a monotonous coastline and a narrow continental shelf, making it steeply outward, and is a region where strong thermoelectric wires from which the North Korean and Korean Waves flow are introduced. In the East Sea, where the average depth is about 1,700m, the deep sea is deeper than 200m in depth, and it is known to have low oxygen temperature, high salinity, and stable marine environment characteristics of the matte layer, and it is known that inhabitants with low oxygen metabolic rate inhabit because oxygen is not produced by photosynthesis. . The development, management, and preservation of deep-sea fishery have been carried out through the recent active development of the fisheries for the deep sea of the East Sea, and the production of fish such as snow crabs, red snow crabs, northern pink shrimps, and crustaceans, scallions, and tuna is also increasing.

However, the industry and research on the deep sea creatures in the East Sea of Korea are limited to the ecological, classification, and resource epidemiological properties of crustaceans and fish, which are mostly used as food. As a result, studies on invertebrates in which various bioactive substances are produced are insufficient. This is true.

Invertebrates have a well-developed innate immunity system to protect themselves, producing many types of secondary metabolites to resist excretion of waste and microbial infection. These secondary metabolites have anti-viral, anti-bacterial, anti-cancer, and anti-inflammatory effects, and are used as a variety of biologics.

In particular, the starfish, one of the most common marine animals that we can encounter, is a representative invertebrate, inhabiting over 30 species on the coast of the country and its breeding rate is very high, and it is known to spawn 2 million to 3 million birds per bird.

Recently, researches on the use of starfish as health foods, medicines, and industrial ingredients for human consumption have been actively conducted. In particular, research on pharmaceuticals is the most active field, and calcium supplements and extracts using starfish's calcium components are extracted. Studies on various anti-cholesterol agents, antibacterial compositions, and the like have been actively conducted.

For example, Patent Publication No. 10-2017-0097822 uses a starfish extract fermented with a mushroom mycelium as an antioxidant composition. However, the patent has a problem in that it is difficult to secure a sufficient yield since an additional fermentation process using Cordyceps sinensis is required to obtain the antioxidant effect of the starfish extract.

In addition, studies on the antioxidant and anti-inflammatory effects of various types of starfish extracts are insufficient, and studies to utilize them for food, cosmetics, and pharmaceutical use are still insufficient.

Patent Publication No. 10-2017-0097822

The present invention is to provide a composition and a method of manufacturing the same as an active ingredient containing an extract of a dwarf starfish ( Crossaster papposus japonicus ) having excellent anti-inflammatory activity and antioxidant activity, and an extract of a dwarf starfish is ABTS radical, and Hydrogen It is to provide a food composition, a cosmetic composition and a pharmaceutical composition containing the peroxide (H ₂ O ₂ ) removal activity is excellent and confirming that it has a nitrogen monoxide (NO) production inhibitory activity.

The present invention provides a composition for anti-oxidation and anti-inflammatory, which contains the extract of Cross Starfish ( Crossaster papposus japonicus ) as an active ingredient.

The polyphenol content of the dwarf starfish extract may be 10 mg / g or more.

The dwarf starfish extract may be extracted with any one solvent selected from water, methanol, hexane, chloroform, alcohol, gastrointestinal hydrolase, and mixtures thereof.

As an example, the dwarf starfish extract,

Including the process of mixing dry powder of dwarf starfish and 60 to 80% ethanol in a ratio of 1: 5 to 15 (w / w), extracting it several times at room temperature for 20 to 28 hours, and filtering under reduced pressure and freeze-drying. Can be manufactured.

As another example, the dwarf starfish extract,

Prepared by mixing dry powder of dwarf starfish and water in a ratio of 1: 5 ~ 15 (w / w), extracting it several times at 70-110 ° C for 2 to 4 hours, filtering under reduced pressure, and freeze-drying Can be.

As another example, the dwarf starfish extract,

(A) mixing the dry powder of dwarf starfish and water in a ratio of 1: 40 to 60 (w / w);

(B) mixing the mixture of step (A) and the first gastrointestinal hydrolase at a ratio of 1: 200 to 300 (w / w) under conditions of pH 1 to 3, followed by treating for 2 to 4 hours;

(C) After mixing the treated product of step (B) and the second gastrointestinal hydrolase and the third gastrolytic hydrolase at pH 7.5 to 8.5 in a ratio of 1: 400 to 600 (w / w) 2 Treating for 4 hours; And,

(D) It may be prepared by including the process of filtration and freeze-drying the treated product of step (c).

The first gastrointestinal hydrolase may be pepsin, the second gastrointestinal hydrolase may be trypsin, and the third gastrointestinal hydrolase may be alpha-chymotrypsin.

The present invention provides a food composition comprising the antioxidant and anti-inflammatory composition.

The present invention provides a cosmetic composition comprising the composition for antioxidant and anti-inflammatory properties.

The present invention provides a pharmaceutical composition comprising the composition for antioxidant and anti-inflammatory.

In addition, the present invention provides a method for preparing an antioxidant and anti-inflammatory composition containing the extract of the above-mentioned dwarf starfish ( Crossaster papposus japonicus ) as an active ingredient.

The dwarf starfish extract may be extracted with any one selected from water, methanol, hexane, chloroform, alcohol, gastrointestinal hydrolase, and mixtures thereof.

The composition for anti-oxidation and anti-inflammatory according to the present invention contains an extract of a dwarf starfish ( Crossaster papposus japonicus ) as an active ingredient, has excellent ABTS radical, and hydrogen peroxide (H ₂ O ₂ ) removal activity and high nitrogen monoxide (NO) It shows a production inhibitory activity, so it has excellent antioxidant and anti-inflammatory effects. In addition, it has been found that there is no toxicity to the cells, which may be useful as a food composition, cosmetic composition, or pharmaceutical composition.

1 is a graph showing the measurement results of ABTS radical scavenging activity in Experimental Example 2;
Figure 2 is a graph showing the measurement results of the hydrogen peroxide (H ₂ O ₂ ) scavenging activity of Experimental Example 3;
3 is a graph showing the cytotoxicity evaluation results of Experimental Example 4; And,
4 is a graph showing the measurement results of the nitrogen monoxide (NO) production inhibitory activity of Experimental Example 5.

The present invention,

Provided is a composition for antioxidant and anti-inflammatory, which contains the extract of Cross Starfish ( Crossaster papposus japonicus ) as an active ingredient.

The dwarf starfish is distributed on the coast of the East Sea of Korea, and is a relatively rare medium-sized starfish of about 7cm in length, including the arm, which is sometimes found at a depth slightly around 100m. It contains minerals and vitamins such as protein, calcium, and phosphorus, and especially contains a large amount of phenolic substances having efficacy against various diseases.

In the present invention, "active ingredient" is a substance or group of substances (pharmacologically active ingredient, etc.) expected to directly or indirectly express the efficacy and effect of the composition, for example, antioxidant and anti-inflammatory effects, by the inherent pharmacological action. (Including unsupported herbal medicines, etc.).

In the present invention, "antioxidant and anti-inflammatory composition" means a composition that simultaneously exhibits antioxidant and anti-inflammatory effects.

Antioxidant and anti-inflammatory composition of the present invention, the composition of the weight relative to the total weight of the starfish extract may contain 0.0001% to 99.99% by weight, specifically 0.1% to 99% by weight, the antioxidant and anti- The content of the active ingredient can be appropriately adjusted according to the use method and purpose of the inflammatory composition.

The polyphenol content of the dwarf starfish extract may be 10 mg / g or more, and specifically, 10 mg / g or more to 30 mg / g or less.

The dwarf starfish extract can be prepared using various extraction solvents. For example, the extraction solvent may be any one selected from water, methanol, hexane, chloroform, alcohol, gastrointestinal hydrolase, and mixtures thereof, specifically, water, alcohol, gastrointestinal hydrolase, and mixtures thereof It may be one or more selected from.

The water may be, for example, distilled water, or ultra pure water, and may be used in the form of cold water or hot water, but is not limited thereto.

The alcohol is a kind of ethanol, for example, may be 60 to 80% ethanol, but is not limited thereto.

The gastrointestinal hydrolase is a proteolytic enzyme secreted by the stomach or intestine, and may be, for example, pepsin, trypsin, or alpha-chymotrypsin, but is not limited thereto.

The method of obtaining the dwarf starfish extract is not limited, but the inventors of the present invention confirmed that the extract obtained under specific extraction conditions exhibits excellent antioxidant and anti-inflammatory effects, as shown in the following experimental examples.

As an example, the dwarf starfish extract,

Including the process of mixing dry powder of dwarf starfish and 60 to 80% ethanol in a ratio of 1: 5 to 15 (w / w), extracting it several times at room temperature for 20 to 28 hours, and filtering under reduced pressure and freeze-drying. Can be manufactured.

Specifically, after mixing the dry powder of dwarf starfish and 60 to 80% ethanol in a ratio of 1: 8 to 12 (w / w), extract 3 to 5 times at room temperature for 22 to 26 hours and filter under reduced pressure and And freeze-drying.

The dry powder of the dwarf starfish can be prepared according to a conventional method known in the art, and specifically, it can be obtained by washing, drying and pulverizing the dwarf starfish.

As another example, the dwarf starfish extract,

Prepared by mixing dry powder of dwarf starfish and water in a ratio of 1: 5 ~ 15 (w / w), extracting it several times at 70-110 ° C for 2 to 4 hours, filtering under reduced pressure, and freeze-drying Can be.

Specifically, after mixing the dry powder and water of the dwarf starfish in a ratio of 1: 8 to 12 (w / w), extract 3 to 5 times at 80 to 100 ° C. for 3 hours, and then filter under reduced pressure and freeze-dried. It can be manufactured, including the process.

As another example, the dwarf starfish extract,

(A) mixing the dry powder of dwarf starfish and water in a ratio of 1: 40 to 60 (w / w);

(B) mixing the mixture of step (A) and the first gastrointestinal hydrolase at a ratio of 1: 200 to 300 (w / w) under conditions of pH 1 to 3, followed by treating for 2 to 4 hours;

(C) After mixing the treated product of step (B) and the second gastrointestinal hydrolase and the third gastrolytic hydrolase at pH 7.5 to 8.5 in a ratio of 1: 400 to 600 (w / w) 2 Treating for 4 hours; And,

(D) It may be prepared by including the process of filtration and freeze-drying the treated product of step (c).

In detail, the step (B) is mixed with the mixture of the step (A) and the first gastrointestinal hydrolase at a pH ratio of 1: 250 to 300 (w / w) and treated for 2 to 4 hours. You can proceed.

Specifically, in the step (c), the treated product of the step (b) and the second gastrointestinal hydrolase and the third gastrointestinal hydrolase are each 1: 500 to 600 (w / w) at pH 8 conditions. After mixing at a ratio, treatment may be performed for 2 to 4 hours.

The first gastrointestinal hydrolase of step (B) is pepsin, the second gastrointestinal hydrolase of trypin is trypsin, and the third gastrolytic hydrolase is alpha chimodlipsin (α -chymotrypsin).

On the other hand, the present invention provides a food composition characterized in that it comprises the composition for antioxidant and anti-inflammatory.

The food composition contains an extract of dwarf starfish and has antioxidant and anti-inflammatory functions, and the composition for antioxidant and anti-inflammatory is a natural component and is not harmful to the human body as it is confirmed that there is no cytotoxicity as can be seen in the experimental examples below. No, it can be safely applied to food.

The food composition of the present invention, as an active ingredient, may include additives commonly used in food compositions in addition to dwarf starfish extract, for example, mixed with other active ingredients such as commonly used additives, additives such as pigments, surfactants, and preservatives Can be used. The additive may be used in the form of a dietary supplement, nutritional supplement, health food, or food additive by pulverizing, granulating, encapsulating, tableting or liquefying depending on the purpose and use of the additive.

The food composition may appropriately control the content of the dwarf starfish extract according to the application object, form, etc., and may be 0.01 to 50% by weight based on the total weight of the composition.

In addition, the present invention provides a cosmetic composition comprising the composition for antioxidant and anti-inflammatory.

The cosmetic composition contains an extract of dwarf starfish and has antioxidant and anti-inflammatory functions, and the composition for antioxidant and anti-inflammatory is a natural component and is not harmful to the human body as it is confirmed that there is no cytotoxicity as can be seen in the following experimental examples. No, it can be safely applied to cosmetics.

The cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the wrinkled starfish extract as an active ingredient, for example, stabilizers, solubilizers, conventional adjuvants such as vitamins, pigments and fragrances, and carriers. can do.

The cosmetic composition may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing oils, It may be formulated as a powder foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto, and specifically, flexible lotion, gel, water-soluble liquid, milk lotion, cream, essence, skin toner, oil-in-water type (oil-in-water) emulsion, water-in-oil emulsion, cleansing foam, cleansing water, pack, body oil, body lotion, oil-in-water makeup base, water-in-oil makeup base and foundation formulation You can.

The cosmetic composition may appropriately adjust the content of the dwarf starfish extract according to the application object, form, etc., in detail, may be 0.01 to 50% by weight relative to the total weight of the composition.

In addition, the present invention provides a pharmaceutical composition characterized in that it comprises the composition for antioxidant and anti-inflammatory.

The pharmaceutical composition contains an extract of dwarf starfish and has antioxidant and anti-inflammatory functions, and the composition for antioxidant and anti-inflammatory is a natural component and is confirmed to be free from cytotoxicity, as can be seen in the following experimental examples. Since it does not, it can be safely applied to medicines.

The pharmaceutical composition may further include additional components, for example, may further include at least one of a carrier, an excipient, and a diluent.

The pharmaceutical composition of the present invention can be used in the form of an oral dosage form such as powder, granule, tablet, capsule, suspension, emulsion, syrup, aerosol, external preparation, suppository, and sterile injectable solution, respectively, according to a conventional method. Examples of carriers, excipients, and diluents that may be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, and cellulose , Methyl cellulose, microcrystalline cellulose, talc, magnesium stearate and mineral oil, but is not limited thereto.

The pharmaceutical composition may appropriately adjust the content of the dwarf starfish extract according to the application object, form, etc., in detail, may be 0.01 to 50% by weight relative to the total weight of the composition.

On the other hand, the present invention provides a method for producing an antioxidant and anti-inflammatory composition containing the above-mentioned dwarf starfish extract as an active ingredient.

The dwarf starfish extract can be extracted with any one solvent selected from water, methanol, hexane, chloroform, alcohol, gastrointestinal hydrolase, and mixtures thereof, and specifically, water, alcohol, gastrointestinal hydrolase is used. Thus, the method described above can be used.

Hereinafter, examples of the present invention will be described in detail. However, the following examples are only to illustrate the present invention, the content of the present invention is not limited to the following examples.

Example 1 (70% ethanol extraction)

The dry powder of dwarf starfish was mixed with 70% ethanol at a ratio of 1: 10 (w / w) and repeatedly extracted three times for 24 hours at room temperature. The extract was centrifuged for 20 minutes to remove residue and filtered under reduced pressure. The obtained filtrate was volatilized with ethanol using a rotary vacuum concentrator, and the concentrate was freeze-dried to obtain an extract sample.

Example 2 (hot water extraction)

The dry powder of dwarf starfish was mixed with distilled water in a ratio of 1: 10 (w / w), and then repeatedly extracted three times for 3 hours in a 90 ° C water heater. The extract was centrifuged for 20 minutes to remove residue and filtered under reduced pressure. The obtained filtrate was freeze-dried to obtain a wrinkled starfish extract.

Example 3 (gastric hydrolase extraction)

After mixing the dry powder of dwarf starfish with distilled water at a ratio of 1:50 (w / w), treat the Pepsin hydrolase at pH 2 in a 37 ° C water bath at a ratio of 1: 250 for 3 hours, trypsin at pH 8, Each of α-chymotrypsin hydrolase was treated at a ratio of 1: 500 for 3 hours. The extract was centrifuged for 20 minutes to remove the residue, and the filtrate obtained through the desalination process was freeze-dried using a table-top electrodialysis apparatus to obtain a dwarf starfish extract.

Experimental Example 1 (component composition measurement)

(1) Test method

The protein content, the total polyphenol content, and the total sugar content of the extract of dwarf starfish obtained in Examples 1 to 3 were investigated in the following manner, respectively, and are shown in Table 1 below.

Protein content

20 uL of each sample was dispensed into a 96 well plate, and 180 uL of BCA assay reagent was added, reacted at 37 ° C for 30 minutes, and absorbance at 540 nm was measured using a micro plate reader. Bovine serum albumin was used as a standard.

Total polyphenol content

After adding 250 uL of 7.5% Na ₂ CO ₃ to 100 uL of each sample, the mixture was reacted at room temperature for 5 minutes. To this, 300 uL of 1N Folin-Ciocalteu reagent was added to react under dark conditions for 30 minutes, and absorbance was measured at 750 nm. Gallic acid was used as a standard material.

Total sugar content

100 uL of each sample was mixed with 100 uL of 5% phenol and 500 uL of H ₂ SO ₄ and reacted at room temperature for 20 minutes. Thereafter, absorbance was measured at 490 nm. Glucose was used as a standard.

(2) Review

<Table 1>

Since ethanol is known as an optimal solvent for extracting biological phenolic substances, alcohol extraction of Example 1 was performed using 70% ethanol to extract phenolic substances having efficacy against various diseases. The hot water extraction of Example 2 is not limited to one thing and is a method capable of extracting various macromolecules contained in the organism. Gastric hydrolase extraction of Example 3 is a method that can be extracted by hydrolyzing the protein of marine invertebrates.

According to Table 1, it can be confirmed that the compositions according to Examples 1 to 3 each include phenolic substances having efficacy against various diseases. Since there was little difference in the content of polyphenols between the alcoholic extract of Example 1 and the hot water extract of Example 2, it was determined that the phenolic substances contained in the deep-sea organisms were traces, and the protein content was higher than that of polyphenols and sugars. Due to the overwhelmingly high level, protein is considered to be the main component of deep-sea invertebrates.

Experimental Example 2 (ABTS radical scavenging activity measurement)

(1) Test method

After mixing 2.45 mM potassium persulphate and 7 mM 2,2'-azino-bis (ethylbenzothiazoline-6-sulfonic aicd) diammonium salt (ABTS) with respect to the extract of dwarf starfish obtained in Examples 1 to 3, at room temperature for 16 hours. By reacting to form ABTS radicals, just before the experiment, the ABTS radical solution was diluted with distilled water to a value of 0.7 ° C ± 0.02 at an absorbance of 735 nm. Then, 100 uL of each sample and 100 uL of ABTS radical solution were reacted in a 96-well plate at room temperature for 10 minutes to measure absorbance at 735 nm. As a control, the absorbance of the sample was measured by adding distilled water instead of the sample, and was calculated by the following equation. The IC ₅₀ value is obtained from the calibration curve for ABTS radical scavenging activity for each concentration, and the concentration value at which the ABTS radical scavenging ability is 50% is obtained and is shown in FIG. 1 below.

ABTS radical scavenging activity (%) = [1- (control absorbance-sample added absorbance) / control absorbance] × 100 (%)

(2) Review

Measurement of ABTS radical scavenging activity is an experiment in which ABTS radical generated by reacting ABTS reagent with potassium persulfate is depleted as cations are eliminated due to the antioxidant activity of the sample. Measurement of ABTS radical scavenging activity is an experiment that can be applied to both hydrophilicity and hydrophobicity, and is an experimental method suitable for natural antioxidant research.

According to the following Fig. 1 showing the results of ABTS radical scavenging activity of the three extracts of dwarf starfish, the IC ₅₀ value, which is 50% eliminated from the ABTS radical gastrointestinal hydrolysis extract of Example 3, shows the lowest value as 0.71 mg / ml. Confirmed.

Experimental Example 3 (Hydrogen peroxide (H ₂ O ₂ ) Erasing ability measurement)

(1) Test method

100 µl of 0.1 M phosphate buffer (pH 5.0) and the dwarf starfish extracts obtained in Examples 1 to 3 were mixed and then dispensed into 96 well plates. 20 µl hydrogen peroxide solution is mixed and added, and reacted at 37 ° C for 5 minutes. After the reaction, 30 µl of 1.25 mM ABTS solution and 30 µl of peroxidase (1 unit / ml) solution are added to the mixture. Then, after reacting at 37 ° C for 10 minutes, absorbance was measured at 405 nm using a micro plate reader, and the results are shown in FIG. 2.

(2) Review

Reactive oxygen species such as hydroxyl radicals produced in living organisms are highly reactive. Superoxide dismutase (SOD) in vivo converts superoxide to hydrogen peroxide, and catalase removes hydrogen peroxide. Meanwhile, glutathione transferase and glutathione peroxidase detoxify, including electrophilic foreign substances, and remove peroxide produced by SOD. However, when the relaxed oxidative stress occurs in vivo, the cells react by activating these antioxidant mechanisms, but the severe oxidative stress causes cell damage and develops into necrosis and apoptosis.

According to the following Figure 2 showing the results of measuring the activity of H ₂ O ₂ scavenging activity of the three extracts of dwarf starfish according to Examples 1 to 3, the IC ₅₀ value, which is the value at which the gastrointestinal hydrolysis extract of Example 3 is 50% eliminated Since it shows the lowest value as 1.73 mg / ml, it was confirmed that it shows the best antioxidant effect.

Experimental Example 4 (Evaluation of Cytotoxicity)

(1) Test method

Toxicity evaluation was performed with Thiazolyl Bule Tetrazolium Bromide (MTT) assay. RAW 264.7 macrophages were dispensed into 24-well-late at 4 × 105 cells / mL, and then cultured for 24 hours at 5% CO ₂ and 37 ° C. conditions, and then extracted from the wrinkled starfish extracts obtained in Examples 1 to 3, respectively. Pre-treatment at concentrations of 100, 200, and 400 ug / mL was carried out under the same conditions. After 1 hour, lipopolysaccharide (LPS, 0.25 ug / mL) was treated to incubate for an additional 24 hours. After cultivation, 100 µL of MTT solution was added to each well, and additional cultivation was performed for 4 hours in order to form formazan in viable cells. And the MTT solution was removed and 100 uL dimethyl sulfoxide was added to measure the absorbance at 540 nm, and the results are shown in FIG. 3.

(2) Review

In order to proceed with the anti-inflammatory experiment of the extract of the dwarf starfish extract, after processing various concentrations (100, 200, 400 ug / mL) and LPS of the dwarf starfish extracts according to Examples 1 to 3 in RAW 264.7, through the MTT assay Cytotoxicity was confirmed.

According to Figure 3 below, it was confirmed that the dwarf starfish extracts according to Examples 1 to 3 had no toxicity to cells compared to the LPS-free and LPS-treated groups, respectively.

Experimental Example 5 (Measurement of inhibitory activity of nitrogen monoxide (NO) production)

(1) Test method

RAW 264.7 is 4 × 105 cells / mL after dispensing in 24-well-late and incubated at 5% CO ₂ and 37 ° C. for 24 hours, and then extracts the dwarf starfish extracts obtained in Examples 1 to 3, respectively. The cells were pre-treated at concentrations of 200 and 400 ug / mL and cultured under the same conditions. After 1 hour, the LPS was treated and incubated for an additional 24 hours, after reacting 100 uL of the culture medium and 100 uL of the griess reagent for 10 minutes, absorbance at 540 nm was measured and shown in FIG. 4.

(2) Review

A small amount of NO (10 ^-12 mole) secreted under normal conditions plays a number of physiological roles, including neurotransmission, relaxation of vascular smooth muscle, and inhibition of platelet aggregation. However, it is known that NO (10 ^-9 mole), which is secreted at a high concentration in an abnormal state, generates strong hydroxyl radicals and harmful substances in the body, thereby deaminating intracellular DNA, causing damage or inducing apoptosis.

RAW 264.7 was treated with LPS, a polysaccharide present in the Gram-negative bacteria membrane, to induce nitrogen monoxide to analyze the anti-inflammatory efficacy of extracts from deep sea invertebrates.

According to FIG. 4, it was confirmed that the LPS treatment group (+) generated NO about 5 times higher than that of the LPS herd group (-), thereby inducing NO generation according to the LPS treatment. In addition, as a result of confirming NO production inhibition, the alcohol extract of Example 1, in particular, exhibited the best nitrogen monoxide (NO) production inhibitory effect at a concentration of 400 ug / mL, thereby confirming an excellent anti-inflammatory effect.

Statistical analysis

The results of this study were all expressed as mean ± standard deviation. Statistics and analysis were evaluated for significance using One-wat ANOVA-test of SPSS program (SPSS Inc. Ver12.0).

Claims (16)

It contains an extract of Cross Starfish (Crossaster papposus japonicus) as an active ingredient, and the Wrinkle Starfish extract,
(A) mixing the dry powder of dwarf starfish and water in a ratio of 1: 40 to 60 (w / w);
(B) mixing the mixture of step (A) and the first gastrointestinal hydrolase at a ratio of 1: 200 to 300 (w / w) under conditions of pH 1 to 3, followed by treating for 2 to 4 hours;
(C) After mixing the treated product of step (B) and the second gastrointestinal hydrolase and the third gastrolytic hydrolase at pH 7.5 to 8.5 in a ratio of 1: 400 to 600 (w / w) 2 Treating for 4 hours; And,
(D) Food composition for antioxidant and anti-inflammatory, characterized in that it comprises a process of filtration and freeze-drying the processed product of step (C). The food composition for antioxidant and anti-inflammatory according to claim 1, wherein the polyphenol content of the dwarf starfish extract is 10 mg / g or more. delete delete delete delete According to claim 1, wherein the first gastrointestinal hydrolase is pepsin (pepsin), the second gastrointestinal hydrolase is trypsin (trypsin), and the third gastrolytic enzyme is alpha-chimotrypsin (α-chymotrypsin). Characterized by antioxidant and anti-inflammatory food composition. delete The herring starfish ( Crossaster papposus japonicus ) contains an extract as an active ingredient, the herring starfish extract,
(A) mixing the dry powder of dwarf starfish and water in a ratio of 1: 40 to 60 (w / w);
(B) mixing the mixture of step (A) and the first gastrointestinal hydrolase at a ratio of 1: 200 to 300 (w / w) under conditions of pH 1 to 3, followed by treating for 2 to 4 hours;
(C) After mixing the treated product of step (B) and the second gastrointestinal hydrolase and the third gastrolytic hydrolase at pH 7.5 to 8.5 in a ratio of 1: 400 to 600 (w / w) 2 Treating for 4 hours; And,
(D) cosmetic composition for antioxidant and anti-inflammatory, characterized in that it comprises a process of filtering the treated product of step (C) and freeze-drying. The herring starfish ( Crossaster papposus japonicus ) contains an extract as an active ingredient, the herring starfish extract,
(A) mixing the dry powder of dwarf starfish and water in a ratio of 1: 40 to 60 (w / w);
(B) mixing the mixture of step (A) and the first gastrointestinal hydrolase at a ratio of 1: 200 to 300 (w / w) under conditions of pH 1 to 3, followed by treating for 2 to 4 hours;
(C) After mixing the treated product of step (B) and the second gastrointestinal hydrolase and the third gastrolytic hydrolase at pH 7.5 to 8.5 in a ratio of 1: 400 to 600 (w / w) 2 Treating for 4 hours; And,
(D) The pharmaceutical composition for anti-inflammatory, characterized in that it comprises a process of filtering the treated product of step (C) and freeze-drying. delete delete The cosmetic composition for antioxidant and anti-inflammatory according to claim 9, wherein the polyphenol content of the dwarf starfish extract is 10 mg / g or more. 10. The method of claim 9, wherein the first gastrointestinal hydrolase is pepsin, the second gastrointestinal hydrolase is trypsin, and the third gastrointestinal hydrolase is alpha-chymotrypsin. Cosmetic composition for antioxidant and anti-inflammatory. The pharmaceutical composition for anti-inflammatory according to claim 10, wherein the polyphenol content of the dwarf starfish extract is 10 mg / g or more. 11. The method of claim 10, wherein the first gastrointestinal hydrolase is pepsin (pepsin), the second gastrointestinal hydrolase is trypsin (trypsin), and the third gastrolytic enzyme is alpha-chimotrypsin (α-chymotrypsin). Pharmaceutical composition for anti-inflammatory.

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