KR101729654B1

KR101729654B1 - An antibacterial composition comprising extracts of carex humilis

Info

Publication number: KR101729654B1
Application number: KR1020150146118A
Authority: KR
Inventors: 권만재; 노주원; 정상훈; 함박눈; 윤지호; 김명석
Original assignee: 한국과학기술연구원
Priority date: 2015-10-20
Filing date: 2015-10-20
Publication date: 2017-05-11

Abstract

In this specification, an antimicrobial composition comprising an acid mirror ( Carex humilis ) extract as an active ingredient is disclosed. The composition for antimicrobial use disclosed in this specification is harmless to the human body and has no side effects. It has antibacterial activity against pathogenic bacterium and antibiotic resistant bacterium, so that harmful bacteria encountered in everyday life such as skin bacterium, There is an effect of providing prevention, improvement, or therapeutic effect on infection of infection-causing bacteria.

Description

AN ANTIBACTERIAL COMPOSITION COMPRISING EXTRACTS OF CAREX HUMILIS [0002]

In this specification, an antimicrobial composition comprising an acid mirror ( Carex humilis ) extract as an active ingredient is disclosed.

In general, antibiotics are selective toxicity substances that kill microorganisms and are not toxic to humans or animals and are not inactivated by enzymes or the like in the body. They are mainly used for DNA replication, transcription and translation of genetic information, Transport of electron energy, biosynthesis of cell walls, and the like, thereby exhibiting an effect through a mechanism of inhibiting microbial proliferation.

Sir Alexander Fleming first discovered penicillin in 1929 and Flory and others first separated and purified penicillin from a culture of Penicillium chrysogenum in 1939, Has been known as a miracle medicine by saving the lives of many patients with. Since then, research has been conducted in earnest until 1959, and benzylpenicillin, streptomycin, tetracyclin and macrolide erythromycin, which have been used so far as antibiotics, have been developed . In addition, penicillin was first developed by the synthetic method in 1965 to develop semi-synthetic penicillin, methicillin, and in 1973, cephalosporin-based cephazoline was developed.

However, bacteria resistant to penicillin began to appear. The first penicillin-resistant strains were reported in Australia in 1967, and soon bacterium resistant to methicillin or cephazoline appeared, causing serious problems. A number of antibiotics such as quetiapine, carbapenem, monobactam, and glycosides have been developed and used in clinical practice in the 1980s. In the 1990s, vancomycin-resistant streptococci (VRE) appeared and the problem of antimicrobial resistant bacteria became a major concern in the world. Respectively.

Most antibiotics not only have several chiral centers but also have complex structures such as macrolides or peptides, which are difficult to synthesize, and these synthetic methods also have a very low economic efficiency compared to fermentation methods. In addition, in the recent stringent safety review process, it is often found that they are not able to meet the safety of human body 100%. Therefore, the safety of microbicide is emerging as an urgent problem. In the case of microbial disinfectants, there is a strong tendency to use natural antimicrobial agents which are not harmful to the human body, and the development of nontoxic natural antimicrobial agents is currently being actively promoted due to the wide range of industrial applications.

At present, representative examples of natural antibiotics that can be used include niacin and polylysine, lactic acid bacteriocins. However, they are required to be used in large amounts for antibacterial activity, and they are expensive. In addition, chitosan is more effective against Gram-positive bacteria than Gram-negative bacteria, but exhibits a relatively weak antibacterial activity against fungi and has little effect on genus containing chitosan in the cell wall.

Korean Patent Publication No. 2010-0029870

In one aspect, the present invention aims to provide an antimicrobial composition comprising an acid mirror extract having antimicrobial activity against pathogenic bacteria and antibiotic resistant bacteria as an active ingredient.

In another aspect, the present invention aims to provide an antibacterial composition comprising an acid mirror extract which is harmless to the human body and has no side effects as an active ingredient.

In one aspect, the technique disclosed herein provides an antimicrobial composition comprising an acid mirror ( Carex humilis ) extract as an active ingredient.

In one exemplary embodiment, the composition is comprised of Staphylococcus aureus , Staphylococcus epidermidis , and methicillin resistant staphylococcus aureus (MRSA). And the antimicrobial activity against one or more strains selected from the group consisting of < RTI ID = 0.0 >

In an exemplary embodiment, the acid mirror extract may be extracted with one or more solvents selected from the group consisting of water and alcohols having 1 to 6 carbon atoms.

In one exemplary embodiment, the acid mirror extract may be a methanol extract.

In one exemplary embodiment, the acid mirror extract may comprise from 0.02 to 50% by weight, based on the total weight of the composition.

In one exemplary embodiment, the composition may be a pharmaceutical composition for the prevention or treatment of acne or atopy.

In an exemplary embodiment, the composition may be a cosmetic composition for preventing or ameliorating acne or atopy.

In one exemplary embodiment, the composition may be a food composition for preventing or ameliorating acne or atopy.

In an exemplary embodiment, the composition may be for a wet tissue, a detergent, a disinfectant, or an eye drop.

In one aspect, the technique disclosed in this specification is effective in providing an antimicrobial composition comprising an acid mirror extract having antibacterial activity against pathogenic bacteria and antibiotic resistant bacteria as an active ingredient.

In another aspect, the technique disclosed in this specification is effective to provide an antimicrobial composition comprising an acid mirror extract which is harmless to the human body and has no side effects as an active ingredient.

In another aspect, the antimicrobial composition disclosed in the present invention is useful as a composition for preventing, ameliorating or treating a harmful bacterium encountered in everyday life, for example, an infectious bacterium of the skin, a dermatitis- It is effective.

Hereinafter, the present invention will be described in detail.

As used herein, the term "active ingredient" refers to a component that can exhibit the desired activity alone, such as a carrier that exhibits the desired activity alone or is itself inactive.

Mountain Mirror ( Carex humilis Leyss is a perennial plant belonging to the genus Cyperaceae , which grows in shady rock crevices or dry forests. Its height is 6-10 cm, its rhizome is thick and short, its stem is small, and it is a total. Leaves are narrow and long, about 30 cm long, about 1 mm wide, with rough edges and short hairs. The fruit is an achene and is a three-wide wide obovate with a length of 2 mm.

The acid mirror can be used without limitation throughout the entire range of plants such as seeds, roots, stems, leaves or fruits for the production of the extract. More specifically, roots, stems and leaves can be used. In addition, the acid mirror extract can be used without limitation such as extracted or commercially available.

In one exemplary embodiment, the acid mirror extract is meant to include all forms of the extract, as well as further processing of the extract, such as drying, concentration, fractionation, fermentation and the like.

In one exemplary embodiment, the acid mirror extract may be an acid mirror itself or an extract obtained by powdering it or through an extraction process. The extraction process may be carried out according to a conventional method used in the art.

Specifically, water, anhydrous or hydroalcohol having 1 to 6 carbon atoms (such as methanol, ethanol, propanol or butanol), propylene glycol, butylene glycol, dipropylene glycol, glycerin, acetone, ethyl acetate, chloroform, methylene chloride, butyl May be extracted using a solvent extraction method using an extraction solvent selected from the group consisting of acetone, diethyl ether, dichloromethane, hexane, and mixtures thereof, supercritical extraction, ultrasonic extraction, or fermentation using natural microorganisms or natural fermentation . The extraction solvent is not limited to the extraction solvents listed above. The specific use amount of the extraction solvent may be 5 to 100 times by weight of each sample to be extracted, and the extraction time may be 2 to 24 hours, or 3 to 10 hours.

In one exemplary embodiment, the acid mirror extract may be comprised between 0.02 and 50 wt%, based on the total weight of the composition. Since the acid mirror extract is contained in the above range within the composition, it has an effect of exhibiting an excellent antimicrobial activity with an appropriate composition ratio with other contained materials, and may be preferable from the viewpoint of economy and efficiency. Specifically, the acid mirror extract is used in an amount of 0.02 to 40% by weight, or 0.02 to 30% by weight, or 0.02 to 20% by weight, or 0.02 to 15% by weight, or 0.02 to 10% 10 to 10% by weight, or 1.0 to 10% by weight, or 1.0 to 5% by weight.

The acne bacterium, which is the cause of acne, has a lipophilic nature and can hardly grow without sebum. Therefore, the growth of acne bacterium is limited to many lipids. The major strains causing acne include P. aeruginosa , S. epidermidis , S. aureus , and P. acnes . These strains mainly cause skin troubles, acne, atopy, inflammation, And is used as a target strain for evaluating the antimicrobial activity of acne and atopic treatment and improvement compositions. On the other hand, in patients with atopic dermatitis, the skin is dry and prone to damage, and bacteria and antigens are easily invaded. The most common strain is S. aureus , which has been reported to be detected in more than 90% of patients with atopic dermatitis. Therefore, antimicrobial agents have an important role in preventing deterioration of atopic dermatitis symptoms and preventing secondary infection.

In one exemplary embodiment, the composition may be a pharmaceutical composition for the prevention or treatment of acne or atopy, and the pharmaceutical composition is meant to include a skin external composition.

The pharmaceutical composition may be in the form of a solution, a suspension, a milky lotion, a gel, a drip, a suppository, a cream, an ointment, a patch, a pad or a spraying agent. The formulations can be readily prepared according to conventional methods in the art and can be prepared by conventional means such as excipients, wetting agents, emulsifying accelerators, suspending agents, salts or buffers for controlling osmotic pressure, coloring agents, spices, stabilizers, preservatives, Adjuvants may be used as appropriate.

The pharmaceutical composition may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) according to a desired method, and the active ingredient of the pharmaceutical composition may be administered orally, Weight, condition and severity thereof, route of administration, or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, 0.000025 mg / g / day to 0.025 mg / g / day, more specifically 0.00025 mg / g / day. < / RTI >

The formulation of the cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, it can be applied to softeners (skin lotion and milk lotion), nutrition lotion, essence, nutrition cream, massage cream, pack, gel, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, A body lotion, a body cream, a body oil, and a body essence, but the present invention is not limited thereto.

The above-mentioned cosmetic composition may further contain other ingredients which can give a synergistic effect to the main effect, as long as it does not impair the main effect. The cosmetic composition may further include a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The blending amount of the above components can be easily selected and used by those skilled in the art within a range not to impair the objects and effects of the present invention. The blending amount thereof is 0.01 to 5% by weight, or 0.01 to 3% .

The food composition provides various forms of food additives or functional foods. Specifically, the composition can be processed into an extruded tea, a liquid tea, a beverage, a fermented milk, a cheese, a yogurt, a juice, a probiotic agent or a health supplement, etc., and can be used in various other food additives.

The food composition may further contain other ingredients and the like that can give a synergistic effect to the main effect within a range in which the active ingredient does not impair the intended main effect. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oiliness vitamins, high molecular weight peptides, polymeric polysaccharides and seaweed extract. The ingredients may be selected and mixed by the person skilled in the art without difficulty depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present specification. For example, the amount of addition of the components may range from 0.01 to 5% by weight, or from 0.01 to 3% by weight, based on the total weight of the composition.

The composition disclosed herein can be applied to any one or more selected from the group consisting of purified water, a moisturizer, a surfactant, a pH adjuster, a vegetable preservative, and the like as well as nonwoven fabric and pulp, which are main components for application to antibacterial wet tissues, It is not.

The detergent described in the present specification is a substance used for the purpose of cleaning animal or vegetable oil or foreign matter adhered to a surface, and specifically includes a soap, a shampoo, a hand cleaner, a body cleaner, a kitchen cleaner, a bathroom cleaner, a bottle cleaner, a humidifier cleaner, Cleaning agent and the like.

The bactericide disclosed in the present specification means a substance having an effect of killing microorganisms or inhibiting the growth, and specifically includes a disinfectant for the purpose of preventing infection.

The eyedrops disclosed in the present specification mean drugs administered to the eye that effectively prevent the propagation of bacteria and have an effect of preventing, improving or treating ocular infection-causing bacteria and eye disease-related bacteria.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Example . Preparation of strains for acid mirror extract and antibacterial activity test

The methanol extract of the outbreak of Carex humilis was purchased from the Plant Extract Bank of Korea Research Institute of Bioscience and Biotechnology. The extract was dissolved in dimethyl sulfoxide (DMSO) and used for the experiment.

Examples of strains for the antimicrobial activity test of the acid mirror extract include Staphylococcus aureus , Staphylococcus epidermidis , methicillin resistant staphylococcus aureus (MRSA) Were purchased from KCTC and used for experiments.

Test example. Antimicrobial activity test by disk diffusion method

In order to confirm the antimicrobial activity of the acid mirror extract of the above example, the disk diffusion method was performed as described below.

Specifically, Staphylococcus aureus , Staphylococcus epidermidis , and methicillin resistant staphylococcus aureus (MRSA) were each cultured in liquid medium for 24 hours in the presence of 1 / 10, and 200 [mu] l of the solution was spread on agar medium. Then, 3 쨉 l of a solution obtained by dissolving the extract in DMSO to a concentration of 20 ㎎ / ㎖ of the extract prepared in the examples was soaked in a round filter paper having a diameter of about 8 mm and then spread on an agar medium to be spread on an agar medium. The results of the antimicrobial activity measurement are shown in Table 1 below by measuring the clear zone in which bacterial growth was inhibited after incubation at 30 ° C or 37 ° C for 24 hours.

Table 1. Antimicrobial activity of each strain according to the size of the transparent zone

* Control: DMSO

** -: No suppression effect, +: less than 1mm, ++: more than 1mm, less than 2mm, +++: more than 2mm

As a result, it was found that the acid mirror extract exhibits a wide transparent zone or has a high antimicrobial activity with little growth of bacteria. As can be seen from Table 1, the acid mirror extract showed strong antimicrobial activity particularly in Staphylococcus epidermidis and Staphylococcus aureus , and showed excellent antimicrobial activity against MRSA 2659 and MRSA 9561 with almost no growth of bacteria.

Accordingly, it has been found that the acid mirror extract disclosed in this specification is excellent in antimicrobial effect and has no side effects, so that it can be effectively used for the purpose of removing bacteria.

Formulation examples of compositions according to one aspect of the present disclosure are described below, but are also applicable to various other formulations, which are not intended to be limiting but merely illustrative of the invention.

[Formulation Example 1] Soft capsule

A soft capsule was prepared by mixing 0.0025 g of an acid mirror extract, 50 mg of red ginseng extract, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow pearls and 6 mg of lecithin and filling each 400 mg per capsule according to a conventional method.

[Formulation Example 2] Tablets

Mixed with 0.0025 g of acid mirror extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate, 40 mg of 30% ethanol was added to form granules, followed by drying at 60 ° C., Tablets were tableted.

[Formulation Example 3] Granules

Mixed with 150 mg of acid mirror extract, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch, 100 mg of 30% ethanol was added to form granules and dried at 60 ° C to form granules. The final weight of the contents was 1 g.

[Formulation Example 4] Drinking agent

0.005 g of acid mirror extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled into bottles. The final volume of the contents was adjusted to 200 ml.

[Formulation Example 5] Health food

Mountain mirror extract ............ 0.0025 g

Vitamin mixture

Vitamin A acetate ......... 70 ㎍

Vitamin E .................. 1.0 mg

Vitamin B1 .................. 0.13 mg

Vitamin B2 ... 0.15 mg

Vitamin B6 .................. 0.5 mg

Vitamin B12 ... 0.2 mg

Vitamin C .................... 10 mg

Biotin ...................... 10 μg

Nicotinic acid amide 1.7 mg

Folic acid ........................ 50 ㎍

Calcium pantothenate .............. 0.5 mg

Mineral mixture

Ferrous sulfate .................. 1.75 mg

Zinc oxide ................... 0.82 mg

Magnesium carbonate 25.3 mg

Potassium phosphate 15 mg

Secondary calcium phosphate ................. 55 mg

Potassium citrate .................. 90 mg

Calcium carbonate .................... 100 mg

Magnesium chloride ............ 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

[Formulation Example 6] Health drinks

Mountain Mirror Extract .............. 0.0025 g

Citric acid ..................... 1000 mg

Oligosaccharides ................... 100 g

Plum concentrate ................. 2 g

Taurine ..................... 1 g

Purified water was added to the entire mixture. 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The resulting solution was filtered to obtain a sterilized 2 liter container, sealed sterilized, &Lt; / RTI >

Although the composition ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand level, demand country, use purpose, and the like. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims

( Carex humilis ) extract as an active ingredient,
One or more strains selected from the group consisting of Staphylococcus aureus , Staphylococcus epidermidis , and methicillin resistant staphylococcus aureus (MRSA) Gt; antimicrobial < / RTI >

delete

The method according to claim 1,
Wherein the acid mirror extract is extracted with at least one solvent selected from the group consisting of water and an alcohol having 1 to 6 carbon atoms.

The method of claim 3,
Wherein said acid mirror extract is a methanol extract.

The method according to claim 1,
Wherein the acid mirror extract is contained in an amount of 0.02 to 50% by weight based on the total weight of the composition.

A pharmaceutical composition for preventing or treating acne or atopy comprising the composition of claim 1

A cosmetic composition for improving acne or atopy comprising the composition of claim 1

A composition for improving acne or atopy comprising the composition of claim 1

The method according to claim 1,
Wherein the composition is for a wet tissue, a cleaning agent, a bactericide, or an eye drop.