KR102386077B1 - Antimicrobial composition comprising extract of Melandryum firmum as an active ingredient - Google Patents

Abstract

The present invention relates to an antibacterial composition comprising Jangguchae extract as an active ingredient, and more particularly, to an antibacterial composition comprising Jangguchae extract exhibiting excellent antibacterial activity against bacteria, fungi and yeast as an active ingredient.
According to embodiments of the present invention, Jangguchae extract exhibits a wide spectrum of antibacterial activity against various bacteria, and is a natural antibiotic that does not cause toxicity or irritation to animals and the human body. , can be usefully used for sterilization, antibacterial, and preservative purposes in various fields such as pesticides.

Description

Antimicrobial composition comprising extract of Melandryum firmum as an active ingredient

The present invention relates to an antibacterial composition comprising Jangguchae extract as an active ingredient, and more particularly, to an antibacterial composition comprising Jangguchae extract exhibiting excellent antibacterial activity against bacteria, fungi and yeast as an active ingredient.

Microorganisms are everywhere around us. In particular, phones, cell phones, flooring materials, refrigerators, air conditioners, humidifiers, toys, wallpaper, and various gas kits that we can easily encounter in our daily life are prone to various bacteria and molds and cause odors, and even the human body. In some cases, harmful bacteria multiply and cause various diseases, and serious social problems such as food poisoning caused by pathogenic E. coli O-157 and Legionella bacteria that cause pneumonia in bathrooms also occur. Therefore, there is a need for a high-functional antibacterial material to prevent this, and many studies on these antibacterial materials are being conducted until now.

Direct or indirect damage caused by pathogenic microorganisms causes many economic, environmental and medical problems. Many problems throughout society, such as loss due to spoilage in the food distribution process in the food industry, harmfulness to the human body and environmental pollution due to the use of excessive chemical pesticides on crops in the agricultural industry, and the emergence of antibiotic-resistant strains due to the misuse of antibiotics are causing them

In particular, methicillin-resistant Staphylococcus aureus is a bacterium that is resistant to various antibiotics and causes various incurable diseases by infecting humans. It is also called multidrug-resistant Staphylococcus aureus or 'oxacillin-resistant Staphylococcus aureus'. By definition, Staphylococcus aureus that has acquired resistance to β-lactam antibiotics including penicillin and cephalosporins becomes methicillin-resistant Staphylococcus aureus (MRSA). MRSA can be particularly problematic in hospitals and nursing homes, where patients with open wounds, using surgical instruments, and weakened immune systems are known to have a higher risk of infection and higher mortality than the general public.

In addition, awareness of the importance of the microbial contamination problem of cosmetics is increasing because of the hygienic aspect of the skin area as well as the deterioration of the product value due to the deterioration of the product itself. Cosmetics are prone to contamination by microorganisms such as mold, yeast, and bacteria in the distribution process or during use by consumers because a lot of nutrients derived from natural products and raw materials that are easily decomposed by microorganisms are used. In order to suppress it, a preservative is added, and the maximum antibacterial activity should be exhibited in a minimum amount. Therefore, it is not only necessary to consider the combination of nutrients in cosmetic products, pH, emulsified form, mixed use of preservatives, and their stability, but also antibacterial activity and skin irritation for all raw materials in the product in advance by identifying each product. It is very important to choose the most appropriate antiseptic system. And because of the various side effects of synthetic preservatives, the development of natural preservatives is emerging as a major concern in the cosmetic industry.

Accordingly, as a natural material having excellent antibacterial properties against various pathogens, there is an increasing demand for environmentally friendly antibacterial substances that can be applied to pharmaceutical compositions and cosmetic compositions as well as detergents, food preservatives, feed additives, and the like.

The present invention has been devised to solve the above problems, and an object of the present invention is to provide an antibacterial composition comprising an extract of Melandryum firmum as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating infectious diseases comprising an extract of Jangguchae ( Melandryum firmum ) as an active ingredient.

Another object of the present invention is to provide a food composition for preventing or improving infectious diseases comprising an extract of Jangguchae ( Melandryum firmum ) as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for preventing or improving infectious diseases comprising an extract of Jangguchae ( Melandryum firmum ) as an active ingredient.

Another object of the present invention is to provide a preservative composition for feed or food containing an extract of Jangguchae ( Melandryum firmum ) as an active ingredient.

Another object of the present invention is to provide an antibacterial quasi-drug composition comprising an extract of Melandryum firmum as an active ingredient.

The technical problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the above object of the present invention, the present invention provides an antibacterial composition comprising an extract of Melandryum firmum as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a pharmaceutical composition for preventing or treating infectious diseases comprising an extract of Melandryum firmum as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a food composition for preventing or improving infectious diseases comprising an extract of Melandryum firmum as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a cosmetic composition for preventing or improving infectious diseases comprising an extract of Melandryum firmum as an active ingredient.

In order to achieve another object of the present invention, the present invention provides a preservative composition for feed or food containing an extract of Melandryum firmum as an active ingredient.

In order to achieve another object of the present invention, the present invention provides an antibacterial quasi-drug composition comprising an extract of Melandryum firmum as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides an antibacterial composition comprising Jangguchae ( Melandryum firmum ) extract as an active ingredient.

In the present invention, the Jangguchae ( Melandryum firmum ) is a dicotyledonous plant, a biennial plant of the Melandryum family, also referred to as Yeoruchae or Gyungyeongyeoruchae. The height is 30~80cm, the nodes are dark purple, and the leaves are opposite, long oval or broad lanceolate, somewhat hairy, and the edges are flat. It mainly lives in the mountains and fields of Korea, Japan, eastern Siberia, and China. The flowers bloom in July, are white, and hang on the inflorescence. The calyx is shaped like a barrel, the tip is divided into 5, and there are 10 purple veins. Petals are 5, the ends are split by 2, a little longer than the calyx, there are 10 stamens and 1 style divided into 3 parts. The fruit is long egg-shaped, the tip is split into 6 pieces, and the seeds are kidney-shaped. The seeds are used as an aphrodisiac and hemostatic analgesic. Young shoots are made with vegetables. Plants belonging to the genus Jangguchae include Arabidopsis ( Melandryum Apricum ), Pink Jangguchae ( Melandryum Capitarum ), and Melandryum Seoulensis . In an embodiment of the present invention, Jangguchae ( Melandryum firmum ) was used.

In one aspect of the present invention, the Jangguchae extract may be an extract of one or more parts selected from the group consisting of leaves, stems, roots and seeds. Specifically, the Jangguchae extract may be a single extract of leaves, stems, roots or seeds, and may be a mixed extract of 2, 3 or 4 types of parts selected from the group consisting of leaves, stems, roots and seeds. Preferably, in the present invention, the Jangguchae extract may be a mixed extract of two or more parts including leaves and stems, and more preferably, a mixed extract of leaves and stems.

In another aspect of the present invention, the Jangguchae extract may be extracted by a known natural product extraction method. Preferably, extraction may be performed with one or more solvents selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, a subcritical fluid, a supercritical fluid, and a mixture thereof. The organic solvent having 1 to 6 carbon atoms is alcohol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, having 1 to 6 carbon atoms. chloride), hexane, cyclohexane, and petroleum ether may be selected from the group consisting of, but is not limited thereto. Preferably, the extraction solvent of the Jangguchae extract in the present invention may be one extracted with an alcohol having 1 to 6 carbon atoms, and most preferably, it may be extracted with ethanol, but is not limited thereto.

In a preferred embodiment, the Jangguchae extract of the present invention may be an ethanol extract of Jangguchae leaves and stems.

In one aspect of the present invention, the antimicrobial composition may further include an antibiotic. The type of the antibiotic is not particularly limited, but non-limiting examples thereof include ampicillin, oxacillin, vancomycin, gentamicin, ciprofloxacin, norfloxacin. , enrofloxacin, oxytetracycline, streptomycin, cephalosporin and colistin.

In the antimicrobial composition of the present invention, the antibiotic may be included in an amount of 1 to 30% by weight based on the total weight of the composition.

In addition, the antibacterial composition of the present invention may further add auxiliary components in a range that does not affect the antibacterial activity of the Jangguchae extract included as an active ingredient. As the auxiliary component, sodium benzonate, allantoin, sodium propionate, etc. may be used.

The sodium benzoate has an antiseptic effect and acts to increase the antibacterial action of the present composition. The allantoin has a wound healing promoting action that revitalizes the cells of the wound tissue, and acts synergistically with the Jangguchae extract to further promote the repair of inflammation. In addition, the sodium propionate may impart antifungal activity to the present composition as an antifungal substance. The auxiliary components may be used by appropriately adjusting the content within a range that does not affect the antibacterial activity of the Jangguchae extract.

In one aspect of the present invention, the antibacterial composition may exhibit antibacterial activity against at least one selected from the group consisting of bacteria, fungi and yeast. Preferably, the bacterium may be a gram-positive bacterium.

In one aspect of the present invention, the antibacterial composition is Staphylococcus aureus, Bacillus subtilis, Candida albicans, Aspergillus niger and citrus It may exhibit antibacterial activity against at least one selected from the group consisting of blue mold fungus (Penicillium italicum).

The present invention also provides a pharmaceutical composition for preventing or treating infectious diseases comprising an extract of Melandryum firmum as an active ingredient.

In the present invention, the infectious disease may be an infectious disease caused by one or more selected from the group consisting of bacteria, fungi and yeast, and the type is not particularly limited, but abscess, dermatitis, osteoarthritis, bacteremia, pneumonia, toxic shock syndrome, It may be at least one selected from the group consisting of food poisoning, high fever, sepsis, edema disease, mastitis, and enterobacteriaceae.

The pharmaceutical composition of the present invention may further include suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceuticals. In addition, according to a conventional method, it can be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injection solutions. Suitable formulations known in the art are preferably those disclosed in the literature (Remington's Pharmaceutical Science, recently Mack Publishing Company, Easton PA).

Carriers, excipients and diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline Cellulose, polyvinyl pyrrolidone, water, methyl hydroxy benzoate, propyl hydroxy benzoate, talc, magnesium stearate, mineral oil, and the like. When formulating the composition, it is usually prepared using a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, and a surfactant. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient in the composition, for example, starch, calcium carbonate, sucrose, lactose, It is prepared by mixing gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, suppositories, and the like. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, etc. can be used.

The pharmaceutical composition of the present invention provides an amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human as thought by a researcher, veterinarian, doctor or other clinician, that is, the amount of the symptom of the disease or disorder being treated. It can be administered in a therapeutically effective amount, which is an amount inducing remission. It is apparent to those skilled in the art that the therapeutically effective dosage and frequency of administration for the pharmaceutical composition of the present invention will vary depending on the desired effect. Therefore, the optimal dosage to be administered can be easily determined by those skilled in the art, and the type of disease, the severity of the disease, the content of active ingredients and other components contained in the composition, the type of formulation, and the age, weight, and general health of the patient It can be adjusted according to various factors including state, sex and diet, administration time, administration route and secretion rate of the composition, treatment period, and concurrently used drugs. For a desirable effect, the pharmaceutical composition of the present invention may be administered in an amount of 0.001 to 100 mg/kg/day, may be administered once a day, or may be administered in several divided doses.

The pharmaceutical composition of the present invention may be administered to an individual by various routes. Any mode of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebrovascular injection.

The present invention also provides a food composition for preventing or improving infectious diseases comprising an extract of Melandryum firmum as an active ingredient.

When the Jangguchae extract of the present invention is used as a food additive, the Jangguchae extract may be added as it is, or it may be appropriately used according to a conventional method, such as mixed with other foods or food ingredients.

In addition, the mixing amount of the active ingredient, Jangguchae extract, may be suitably changed depending on the purpose of use (prevention, health or therapeutic treatment), and the antibacterial composition is included in an amount of 10 to 40% by weight based on the total weight of the food composition. It is preferable to be

As a specific example, when preparing food or beverage, the Jangguchae extract of the present invention may be added in an amount of 40% by weight or less, preferably 30% by weight or less, based on the raw material. However, when consumed for a long period of time for health and hygiene or health control, it may be added in an amount less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range. there is.

There is no particular limitation on the type of the food. Examples of foods to which the antimicrobial composition of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea , drinks, alcoholic beverages, vitamin complexes, etc., and includes all health foods in the ordinary sense.

When the food composition of the present invention is prepared as a beverage, it may contain additional ingredients such as various flavoring agents or natural carbohydrates like a conventional beverage. Examples of the natural carbohydrate include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; Natural sweeteners such as dextrin and cyclodextrin or synthetic sweeteners such as saccharin and aspartame may be used. The natural carbohydrate may be included in an amount of 0.01 to 10% by weight, preferably 0.01 to 0.1% by weight, based on the total weight of the food composition of the present invention.

In addition to the above, the food composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol , a carbonation agent used in carbonated beverages, and the like. In addition, the composition of the present invention may contain the pulp for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination. Although the additive ratio is not particularly limited, it is preferably included in the range of 0.01 to 0.1% by weight based on the total weight of the food composition of the present invention.

The present invention also provides a cosmetic composition for preventing or improving infectious diseases comprising an extract of Melandryum firmum as an active ingredient.

The cosmetic composition of the present invention may further include conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances, and the like, which are commonly used in cosmetic compositions in addition to the above active ingredients. For example, the cosmetic composition may further include auxiliary components such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, allantoin, etc. .

Since the cosmetic composition of the present invention is basically applied to the skin, it can be prepared in any formulation conventionally prepared with reference to a cosmetic composition in the art. For example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, etc. can be formulated, for example, The present invention is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a mask pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be included as carrier components. there is.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be included as carrier components, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain propellants such as butane and dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer, emulsifier, etc. may be included as carrier components, and specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like may be included.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol, propylene glycol; suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tracanth, and the like may be included.

When the formulation of the present invention is a cleansing agent containing surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether as carrier components Sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerol fatty acid ester, and the like may be included.

The present invention also provides a preservative composition for feed or food containing an extract of Melandryum firmum as an active ingredient.

Food or feed preservatives are additives used to prevent deterioration, spoilage, discoloration and chemical changes of food or feed, and include sterilizers and antioxidants, and inhibit the growth of microorganisms such as bacteria, mold, yeast, etc. , functional antibacterial agents such as inhibiting the growth of decaying microorganisms or sterilizing in cosmetics, pharmaceuticals, etc. Ideal conditions for these preservatives in food or feed should be non-toxic and effective even in small amounts. The Jangguchae extract of the present invention exhibits excellent antibacterial and antifungal activity by directly affecting harmful microorganisms, so it can be widely used as a preservative for food or feed, a cosmetic preservative, and a pharmaceutical preservative.

The present invention also provides an antibacterial quasi-drug composition comprising an extract of Melandryum firmum as an active ingredient.

The quasi-drug composition of the present invention is not limited thereto, but preferably disinfectant, shower foam, gargrin, wet tissue, detergent soap, hand wash, humidifier filler, mask, ointment, patch, or filter filler.

In addition, the present invention also provides an animal feed composition for preventing or improving infectious diseases comprising an extract of Melandryum firmum as an active ingredient.

The animal feed composition of the present invention can prevent or improve diseases caused by pathogenic bacteria in animals by feeding them to livestock, and can reduce methanogenesis.

The animal to which the animal feed composition is administered may be preferably a ruminant, and the type may be a cow, a camel, a deer, a giraffe, and the like. In addition, in the feed additive composition for livestock of the present invention, the livestock may preferably be a ruminant, and the type may be a cow, a camel, a deer or a giraffe, but is not limited thereto.

In the animal feed composition of the present invention, in addition to the above active ingredients, organic acids such as citric acid, humic acid, adipic acid, lactic acid, malic acid, sodium phosphate, potassium phosphate, acid pyrophosphate, polyphosphate (polyphosphate) commonly used in animal feed compositions Phosphates or polyphenols, such as catechin, alpha-tocopherol, rosemary extract, vitamin C, green tea extract, licorice extract, chitosan, tannic acid, natural antioxidants such as phytic acid may be further included.

In addition, the animal feed composition may further include auxiliary ingredients such as amino acids, inorganic salts, vitamins, antibiotics, antibacterial substances, antioxidants, antifungal enzymes, live microorganisms, and the like. Specifically, the auxiliary component includes grains (eg, pulverized or crushed wheat, oats, barley, corn, rice, etc.), vegetable protein feed (eg, those containing rapeseed, soybean, sunflower, etc. as main components), Animal protein feed (e.g., blood meal, meat meal, bone meal, fish meal, etc.), sugar and dairy products (e.g., dry ingredients consisting of various milk powder or whey powder), lipids (e.g., animal fat, vegetable fat, etc.) ), nutritional supplements, digestion and absorption enhancers, growth promoters, and disease prevention agents.

The animal feed composition may be administered to the animal alone or mixed with other feed additives in an edible carrier. In addition, the animal feed composition may be mixed as a top dressing of the feed, and may be easily administered by combining the feed with a separate additive, or as a separate oral formulation. The dosage of the animal feed composition may be appropriately adjusted to a single daily intake or divided daily intake administered in the art.

According to embodiments of the present invention, Jangguchae extract exhibits a broad spectrum of antibacterial activity against various bacteria, and is a natural antibiotic that does not cause toxicity or irritation to animals and the human body. , can be usefully used for sterilization, antibacterial, and preservative purposes in various fields such as pesticides.

1 is a result of evaluation of the antibacterial activity of Jangguchae extract against Aspergillus mold according to the paper disk diffusion analysis method. : Goryanggang)

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments published below, but may be implemented in various different forms, and only these embodiments make the publication of the present invention complete, and common knowledge in the art to which the present invention pertains It is provided to fully inform those who have the scope of the invention, and the present invention is only defined by the scope of the claims. Like reference numerals refer to like elements throughout.

Unless otherwise defined, all terms (including technical and scientific terms) used herein may be used with the meaning commonly understood by those of ordinary skill in the art to which the present invention belongs. In addition, terms defined in a commonly used dictionary are not to be interpreted ideally or excessively unless clearly defined in particular.

In addition, in this specification, the singular form may also include a plural form unless otherwise specified in the phrase. As used herein, "comprises" and/or "comprising" refers to the presence of one or more other components, steps, operations and/or elements mentioned. or addition is not excluded.

Example 1: Preparation of Jangguchae extract

Jangguchae ( Melandryum firmum ) is an annual or biennial herb that grows wild in the mountains and fields of each region of Korea. In Korea, the outpost or seed of Jangguchae is used as a medicinal herb called Wangbulryuhaeng. Jangguchae used for extraction is native to the mountains of Korea, and the outpost containing leaves and stems was used. The dried Jangguchae was first cut into small pieces and then pulverized to increase the solvent extraction efficiency. After adding 1000 ml of ethanol (EtOH) to 100 g of finely pulverized Jangguchae, extraction was performed at room temperature for 1 to 2 days to obtain an extract. The extract was filtered with filter paper (Whatman filter paper No. 2) and concentrated under reduced pressure using a rotary vacuum concentrator to obtain 6 g of crude ethanol extract from Jangguchae.

Example 2: Evaluation of antibacterial activity of Jangguchae extract

Antibacterial activity evaluation of the ethanol extract of Jangguchae obtained in Example 1 was performed using a paper disk diffusion method (Toama et al., 1978). As blood test bacteria used for the evaluation of antibacterial activity, 4 types of bacteria and 3 types of fungi, which are indicators of contamination of cosmetics, were used. Specifically, as bacteria, Staphylococcus aureus , Bacillus subtilis , Pseudomonas aeruginosa , and Escherichia coli were used, and as fungi, Candida was used. Albicans ( Candida albicans ), Aspergillus niger ( Aspergillus niger ), Citrus blue mold bacteria ( Penicillium italicum ) were used.

In the culture of the test bacteria for the evaluation of antibacterial activity, the bacteria are inoculated with 100 cells in a sterile Muller Hinton broth liquid medium and then cultured at 37° C. for 24 hours before incubation, and the number of individuals is 10 ⁵ to 10 After diluting to ⁶ CFU/ml, it was used as an inoculation solution. On the other hand, fungi are cultured 48 to 72 hours at 30°C after inoculation of cells or spores in Potato dextrose broth liquid medium, diluted so that the number of individuals is 10 ⁵ to 10 ⁶ CFU/ml, and used as an inoculation liquid. did. The pre-culture solution of the test bacteria prepared in the above method is mixed with the medium for the upper layer of the assay plate to which 0.1% of agar (Agar) has been added to a concentration of 1%, and then dispensed on the medium for the lower layer of the plate to which 1.5% of the agar (Agar) prepared in advance is added. It was coagulated and used as a plate for antibacterial activity assay.

After dissolving the crude ethanol extract of Jangguchae of Example 1 with 100% ethanol, it was dropped so as to be 1.5 mg/disk on a sterilized 8 mm diameter paper disk (Paper disk, ADVANTEC, Japan), and then raised so as to adhere to the surface of the plate for the assay. Next, the diameter of the growth inhibition ring around the disk was observed while culturing bacteria at 37°C for 24 hours and fungi at 30°C for 48 to 72 hours. The results are shown in Table 1 and FIG. 1 below.

blood test bacteria Antibacterial activity (growth inhibition diameter, mm) Staphylococcus aureus 18 Bacillus subtilis ( Bacillus subtilis ) 10 Pseudomonas aeruginosa ( Pseudomonas aeruginosa ) 0 Escherichia coli 0 Candida albicans 12 Aspergillus niger 40 Citrus blue mold fungus ( Penicillium italicum ) 35

As shown in Table 1 and FIG. 1, the ethanol extract of Jangguchae is Gram-positive bacteria Staphylococcus aureus , Bacillus subtilis and fungi Candida albicans ( Candida albicans ), Aspergillus Aspergillus niger and citrus putrefactive bacteria that frequently occur in cereals, legumes, and fruits during storage as a byproduct mold and accumulate spoilage and toxic substances. It exhibited strong antibacterial activity against phosphorus blue mold fungus ( Penicillium italicum ). However, at the applied concentration of the extract, it did not show antibacterial activity against Gram-negative bacteria.

Considering the above experimental results, it is judged that the ethanol extract of Jangguchae is very useful as a raw material for cosmetics when considering other functions beneficial to skin health as well as having a very high utility value as a preservative material for cosmetics and foods.

As described above, according to the embodiments of the present invention, Jangguchae extract exhibits a broad spectrum of antibacterial activity against various bacteria, and is a natural antibiotic that does not cause toxicity or irritation to animals and the human body, so cosmetics, medicines, feeds, It can be usefully used for sterilization, antibacterial, and preservative purposes in various fields such as food, household goods, and pesticides.

A person of ordinary skill in the art related to this embodiment will understand that it can be implemented in a modified form without departing from the essential characteristics of the above description. Therefore, the disclosed methods are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (17)

Aspergillus niger (Aspergillus niger) or citrus blue mold fungus (Penicillium italicum) antibacterial composition comprising an extract of a herb containing leaves and stems of Jangguchae (Melandryum firmum) as an active ingredient. delete delete The method according to claim 1, wherein the herbal extract comprising the leaves and stems of Jangguchae is an extract using one or more solvents selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, a subcritical fluid, a supercritical fluid, and a mixture thereof. Antibacterial composition, characterized in that. 5. The method of claim 4, wherein the organic solvent having 1 to 6 carbon atoms is alcohol, acetone, ether, benzene, chloroform, and ethyl acetate having 1 to 6 carbon atoms. ), methylene chloride (methylene chloride), hexane (hexane), cyclohexane (cyclohexane) and petroleum ether (petroleum ether), characterized in that selected from the group consisting of antibacterial composition. The antibacterial composition according to claim 1, wherein the Jangguchae extract is obtained by extracting the outpost including leaves and stems of Jangguchae with an alcohol having 1 to 6 carbon atoms. The antimicrobial composition according to claim 1, wherein the antimicrobial composition further comprises an antibiotic. The method of claim 7, wherein the antibiotic is ampicillin, oxacillin, vancomycin, gentamicin, ciprofloxacin, norfloxacin, enrofloxacin ), oxytetracycline (oxytetracycline), streptomycin (streptomycin), cephalosporin (cephalosporin) and an antibacterial composition, characterized in that at least one selected from the group consisting of colistin (colistin). delete delete delete delete delete delete A cosmetic composition for preventing or improving infectious diseases caused by Aspergillus niger or citrus blue mold fungus (Penicillium italicum), comprising as an active ingredient an extract of an herb containing leaves and stems of Melandryum firmum.
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