KR101691509B1

KR101691509B1 - An antibacterial composition containing myriophyllum verticillatum extracts

Info

Publication number: KR101691509B1
Application number: KR1020150070591A
Authority: KR
Inventors: 권만재; 노주원; 김명석; 윤지호; 함박눈
Original assignee: 한국과학기술연구원; 주식회사 제이트로닉스
Priority date: 2015-05-20
Filing date: 2015-05-20
Publication date: 2016-12-30
Also published as: KR20160136702A

Abstract

The present invention discloses an antimicrobial composition comprising a water scouring powder extract as an active ingredient. In one aspect, the present specification has an effect of providing an antimicrobial composition comprising a water scouring extract having antibacterial activity against pathogenic bacteria and antibiotic resistant bacteria as an active ingredient. In another aspect, the present specification has an effect of providing an antimicrobial composition comprising an effective water-scarifier extract which is harmless to the human body and has no side effects.

Description

AN ANTIBACTERIAL COMPOSITION CONTAINING MYRIOPHYLLUM VERTICILLATUM EXTRACTS [0002]

The present invention discloses an antimicrobial composition comprising a water scouring powder extract as an active ingredient.

Since human skin is in direct contact with the surrounding environment, it is easily exposed to bacteria and fungi. Staphylococcus sp. , Streptococcus sp. , Gram-negative bacteria, Pseudomonas aeruginosa , Escherichia coli , and fungi, which are Gram-positive bacteria and Staphylococcus sp. It is known that various bacterial and fungal infections cause various skin diseases.

In general, antibiotics are selective toxic substances that kill microorganisms and are toxic to humans or animals, and are not inactivated by enzymes in the body. This is a destruction of the cell membrane of microorganisms, Inhibition of transcription and translation of information, biosynthesis of cell wall, and the like, thereby exhibiting an effect through the mechanism of inhibiting the proliferation of microorganisms.

Sir Alexander Fleming first discovered penicillin in 1929 and Flory and others first separated and purified penicillin from a culture of Penicillium chrysogenum in 1939, Has been known as a miracle medicine by saving the lives of many patients with. Since then, research has been conducted in earnest until 1959. Benzylpenicillin, streptomycin, tetracyclin and macrolide erythromycin, which have been used so far as antibiotics, have been developed . Penicillin was first developed by the synthetic method in 1965, and semicylline, a semisynthetic penicillin, was developed. In 1973, cephalosporin type cephalosporin was developed.

However, shortly after penicillin was used, bacteria resistant to this began to appear. The first penicillin-resistant strains were reported in Australia in 1967, and bacteria that were resistant to methicillin or cephazoline soon began to appear, causing serious problems. In addition, a number of antibiotics such as quetiapine, carbapenem, monobactam, and glycosides were developed in the 1980s, and many of these antibiotics were first developed in South Africa. Respectively. However, penicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus aureus (MRSA) started to become a problem in the world after this period. In the 1990s, vancomycin-resistant Streptococcus (VRE) It has emerged as a medical concern.

On the other hand, most antibiotics not only have several chiral centers but also have complex structures such as macrolides or peptides, so that their synthesis is difficult. In addition, this synthesis method is also very economical compared with the fermentation method. In addition, in the recent strict safety review process, it is often found that they can not meet the safety of human body 100%. Therefore, the safety of microbicides is emerging as an urgent problem.

Thus, most of the synthetic antibiotics are known to have side effects. Due to excessive abuse of antibiotics, the appearance and resistance rate of resistant bacteria to existing antibiotics are increasing. Especially recently, due to the appearance of super bacteria resistant to antibiotics It is urgently required to develop a new natural antibiotic agent which does not cause resistance by using relatively safe natural materials.

In the case of microbial disinfectants, there is a strong tendency to use natural antimicrobial agents which are not harmful to the human body, and the development of nontoxic natural antimicrobial agents is currently being actively promoted due to the wide range of industrial applications. In addition, modern people are increasingly inclined to prefer natural remedies from natural sources because of their rejection of chemical synthetic products worldwide. There are fields that require the use of natural antimicrobial agents, such as wet tissues and cleansing tissues, which are commonly used in everyday life. Since they are in direct contact with the skin, a harmful processing agent can be absorbed into the human body through the skin This is because there is a risk. As such safety is emphasized, various kinds of safety such as transdermal toxicity, acute toxicity, mutagenicity and the like are required for antimicrobial agents.

At present, representative examples of natural antibiotics that can be used include niacin and polylysine, lactic acid bacteriocins. However, they are required to have a large amount for antibacterial activity, and they are expensive. In addition, chitosan is more effective against Gram-positive bacteria than Gram-negative bacteria, but exhibits relatively weak antibacterial activity against fungi, and has little effect on genus containing chitosan in the cell wall.

Korean Patent Publication No. 10-2011-0115919

In one aspect, the present invention aims to provide an antimicrobial composition comprising, as an active ingredient, a water scouring extract having antimicrobial activity against pathogenic bacteria and antibiotic resistant bacteria.

In another aspect, the present invention aims to provide an antimicrobial composition comprising a water scouring powder extract which is harmless to the human body and has no side effects as an active ingredient.

In one aspect, the technique disclosed herein provides an antimicrobial composition comprising an extract of Myriophyllum verticillatum as an active ingredient.

In one exemplary embodiment, the composition has an antimicrobial activity against at least one strain selected from the group consisting of Staphylococcus sp. , Pseudomonas sp. , And Burkholderia sp. Lt; / RTI >

In one exemplary embodiment, the strain is selected from the group consisting of Staphylococcus aureus , Staphylococcus epidermidis , opportunistic pathogens Pseudomonas aeruginosa , Burkholderia cepacia ) and methicillin-resistant Staphylococcus aureus (MRSA).

In an exemplary embodiment, the water scouring extract may be extracted with at least one solvent selected from the group consisting of water and an alcohol having 1 to 6 carbon atoms.

In one exemplary embodiment, the water scouring extract may be a methanol extract.

In an exemplary embodiment, the water scouring extract may comprise 0.02 to 50% by weight based on the total weight of the composition.

In an exemplary embodiment, the composition may be a pharmaceutical composition for preventing or treating at least one skin disorder selected from the group consisting of atopy, ringworm, psoriasis, athlete's foot, eczema, dermatitis and acne.

In an exemplary embodiment, the composition may be a cosmetic composition.

In one exemplary embodiment, the composition may be a food composition.

In an exemplary embodiment, the composition may be for a wet tissue, a detergent, a preservative, a disinfectant, a toothpaste, or an oral cleanser.

In one aspect, the present specification has an effect of providing an antimicrobial composition comprising a water scouring extract having antibacterial activity against pathogenic bacteria and antibiotic resistant bacteria as an active ingredient.

In another aspect, the present specification has an effect of providing an antimicrobial composition comprising an effective water-scarifier extract which is harmless to the human body and has no side effects.

Hereinafter, the present invention will be described in detail.

As used herein, the term "active ingredient" refers to a component that can exhibit the desired activity alone, such as a carrier that exhibits the desired activity alone or is itself inactive.

The antimicrobial composition can be used for the treatment of noxious bacteria such as skin infections, dermatitis-causing bacteria, bacteremia or sepsis-causing bacteria in immunodeficiency sites, respiratory infections, peritonitis, endocarditis, postoperative eye infections or central nervous system infection- Or prevention of the disease.

In one exemplary embodiment, the strain is selected from the group consisting of Staphylococcus aureus , Staphylococcus epidermidis , opportunistic pathogens Pseudomonas aeruginosa , Burkholderia cepacia ) and methicillin-resistant Staphylococcus aureus (MRSA). The opportunistic pathogens usually mean pathogens that cause disease if they live freely but the immunity of the host is weakened.

The water scrubber (Myriophyllum verticillatum) is a perennial plant which distributed in a dicotyledonous plant belonging to the genus haloragaceae (Haloragaceae) water scrubber, Korea, China, and Eastern Siberia, North Africa, North America, Europe. It grows in ponds and swamps, and underground stems sideways in the mud. The stem is divided into branches, the ends are floated in water, and the length is 50 cm. Leaves are rounded by four, petioles are not, petioles are divided into small pieces, and cracks in the water are thin like fur, but they come out on the water is wide, short and greenish white.

The waterweed ( Myriophyllum verticillatum ) can be used without limitation throughout the entire range of plants such as roots, stems, leaves or fruits thereof for the production of an extract, and specifically, stems and leaves can be used. In addition, the water wool can be used without restrictions such as those cultivated or marketed.

In one exemplary embodiment, the water scouring extract is meant to include all forms of the extract, as well as any form of the extract, such as, for example, drying, concentration, fractionation, fermentation, and the like.

In one exemplary embodiment, the water scouring extract may be water waters itself or an extract obtained by pulverizing it or through an extraction process. The extraction process may be carried out according to a conventional method used in the art.

Specifically, water, anhydrous or hydroalcohol having 1 to 6 carbon atoms (such as methanol, ethanol, propanol or butanol), propylene glycol, butylene glycol, dipropylene glycol, glycerin, acetone, ethyl acetate, chloroform, methylene chloride, butyl May be extracted using a solvent extraction method using an extraction solvent selected from the group consisting of acetone, diethyl ether, dichloromethane, hexane, and mixtures thereof, supercritical extraction, ultrasonic extraction, or fermentation using natural microorganisms or natural fermentation . The extraction solvent is not limited to the extraction solvents listed above. The specific use amount of the extraction solvent may be 5 to 100 times by weight of each sample to be extracted, and the extraction time may be 2 to 24 hours, or 3 to 10 hours.

In an exemplary embodiment, the water scouring extract may comprise 0.02 to 50% by weight based on the total weight of the composition. Since the water scouring powder extract is contained within the above-mentioned range in the composition, it has an effect of exhibiting an excellent antimicrobial activity with an appropriate composition ratio with other containing substances, and may be preferable from the viewpoint of economy and efficiency. Specifically, the water scouring extract may contain 0.02 to 40 wt%, or 0.02 to 30 wt%, or 0.02 to 20 wt%, or 0.02 to 15 wt%, or 0.02 to 10 wt%, or 0.05 to 10 wt% 10 to 10% by weight, or 1.0 to 10% by weight, or 1.0 to 5% by weight.

In one exemplary embodiment, the composition has an antibacterial activity against bacteria and antibiotic resistant bacteria, and has antimicrobial activity against bacterial and antibiotic resistant bacteria on the skin surface, thereby preventing or treating various skin diseases and antibiotic resistance relief . For example, the composition may be a pharmaceutical composition for preventing or treating at least one skin disease selected from the group consisting of atopy, ringworm, psoriasis, athlete's foot, eczema, dermatitis and acne. Herein, the pharmaceutical composition means a composition comprising a composition for external application for skin.

The pharmaceutical composition may be in the form of a solution, a suspension, a milky lotion, a gel, a drip, a suppository, a cream, an ointment, a patch, a pad or a spraying agent. The formulations can be readily prepared according to conventional methods in the art and can be prepared by conventional means such as excipients, wetting agents, emulsifying accelerators, suspending agents, salts or buffers for controlling osmotic pressure, coloring agents, spices, stabilizers, preservatives, Adjuvants may be used as appropriate.

The pharmaceutical composition may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) according to a desired method, and the active ingredient of the pharmaceutical composition may be administered orally, Weight, condition and severity thereof, route of administration, or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, 0.000025 mg / g / day to 0.025 mg / g / day, more specifically 0.00025 mg / g / day. < / RTI >

In an exemplary embodiment, the composition may be a cosmetic composition.

The formulation of the cosmetic composition is not particularly limited and may be appropriately selected according to the purpose. For example, it can be applied to softeners (skin lotion and milk lotion), nutrition lotion, essence, nutrition cream, massage cream, pack, gel, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, A body lotion, a body cream, a body oil, and a body essence, but the present invention is not limited thereto.

The above-mentioned cosmetic composition may further contain other ingredients which can give a synergistic effect to the main effect, as long as it does not impair the main effect. The cosmetic composition may further include a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The blending amount of the above components can be easily selected and used by those skilled in the art within a range not to impair the objects and effects of the present invention. The blending amount thereof is 0.01 to 5% by weight, or 0.01 to 3% .

In one exemplary embodiment, the composition may be a food composition.

The food composition provides various forms of food additives or functional foods. Specifically, the composition can be processed into an extruded tea, a liquid tea, a beverage, a fermented milk, a cheese, a yogurt, a juice, a probiotic agent or a health supplement, etc., and can be used in various other food additives.

The food composition may further contain other ingredients and the like that can give a synergistic effect to the main effect within a range in which the active ingredient does not impair the intended main effect. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oiliness vitamins, high molecular weight peptides, polymeric polysaccharides and seaweed extract. The ingredients may be selected and mixed by the person skilled in the art without difficulty depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present specification. For example, the amount of addition of the components may range from 0.01 to 5% by weight, or from 0.01 to 3% by weight, based on the total weight of the composition.

The composition disclosed herein can be applied to any one or more selected from the group consisting of purified water, a moisturizer, a surfactant, a pH adjuster, a vegetable preservative, and the like as well as nonwoven fabric and pulp, which are main components for application to antibacterial wet tissues, It is not.

The detergent described in the present specification is a substance used for the purpose of cleaning animal or vegetable oil or foreign matter adhered to a surface, and specifically includes a soap, a shampoo, a hand cleaner, a body cleaner, a kitchen cleaner, a bathroom cleaner, a bottle cleaner, a humidifier cleaner, Cleaning agent and the like.

The preservatives disclosed herein are substances for inhibiting physiological activities of microorganisms and inhibiting growth for the purpose of preservation, and specifically means those containing food preservatives, cosmetic preservatives, and wet tissue preservatives.

The bactericide disclosed in the present specification means a substance having an effect of killing microorganisms or inhibiting the growth, and specifically includes a disinfectant for the purpose of preventing infection.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

Test example.

The methanol extract of waterslip using the outpost of Myriophyllum verticillatum was purchased from the Plant Extract Bank of Korea Research Institute of Bioscience and Biotechnology. Each extract was dissolved in dimethyl sulfoxide (DMSO) and used for the experiment.

Staphylococcus aureus , Staphylococcus epidermidis , opportunistic infectious pathogens Pseudomonas aeruginosa and Burkholderia sephacia as strains for the antibacterial activity test of the water scouring extract, ( Burkholderia cepacia ) was purchased from the Korea Genetic Resources Institute (KCTC) and used for the experiment. Methicillin resistant staphylococcus aureus (MRSA) was purchased from Wonju Severance Christian Hospital and used for the experiment.

In order to confirm the antimicrobial activity of Myriophyllum verticillatum extract, a disk diffusion method was performed using the prepared sample and strain.

Specifically, mouse Staphylococcus fringing grown in liquid medium for 24 hours (Staphylococcus aureus), Staphylococcus epidermidis (Staphylococcus epidermidis), opportunity infectious pathogens Rouge Pseudomonas labor (Pseudomonas aeruginosa), Burke holde Ria Sepharose cyano (Burkholderia and methicillin resistant staphylococcus aureus (MRSA) were diluted 1/10 each, and 200 μl of each was diluted in an agar medium. The waterwash extract prepared was dissolved in DMSO to a concentration of 20 mg / ml, and a round filter paper having a diameter of about 8 mm was impregnated with 3 쨉 l of the solution, and the solution was spread on an agar medium to diffuse in an agar medium. The results of the antimicrobial activity measurement are shown in Table 1 below by measuring the clear zone in which bacterial growth was inhibited after incubation at 30 ° C or 37 ° C for 24 hours.

Table 1. Antimicrobial activity of each strain according to the size of the transparent zone

* Control: DMSO

** -: No suppression effect, +: less than 1mm, ++: more than 1mm, less than 2mm, +++: more than 2mm

As a result, it was confirmed that water waxy extract has a antibacterial activity by forming a transparent ring in all the above-mentioned bacteria. That is, when the water scarlet extract was included, it exhibited a wide transparent zone or a high antimicrobial activity, in which bacteria hardly grow. Especially, S. aureus and S. epidermidis showed strong antimicrobial activity. Therefore, it has been found that the water scouring extract disclosed in the present specification is excellent in antimicrobial effect and has no side effects, so that it can be effectively used for the purpose of removing bacteria.

Formulations and formulations of the compositions according to one aspect of the present disclosure are described below, but are also applicable to a variety of other formulations and preparations, which are not intended to be limiting but rather only to illustrate the invention in detail.

[Formulation Example 1] Softening lotion (skin lotion)

Table 2.

[Formulation Example 2] Softening lotion (milk lotion)

Table 3.

[Formulation Example 3] Nourishing cream

Table 4.

[Formulation Example 4] Massage cream

Table 5.

[Formulation Example 5] Pack

Table 6.

[Formulation Example 6] ointment

Table 7.

[Formulation Example 1] Soft capsule

A soft capsule was prepared by mixing 0.0025 g of water scouring powder extract, 50 mg of red ginseng extract, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow pearls and 6 mg of lecithin and filling the capsule with 400 mg per capsule according to a conventional method.

[Formulation Example 2] Tablets

The granules were formed by mixing 0.0025 g of water scouring powder extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate, and 30% ethanol was added thereto, followed by drying at 60 ° C., Tablets were tableted.

[Formulation Example 3] Granules

The granules were formed by mixing 150 mg of water scouring powder extract, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch and 100 mg of 30% ethanol, and dried at 60 ° C to form granules. The final weight of the contents was 1 g.

[Formulation Example 4] Drinking agent

0.0025 g of water scouring powder extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled in a bottle. The final volume of the contents was adjusted to 200 ml.

[Formulation Example 5] Preparation of health food

Water scrubber extract ............................ 0.0025g

Vitamin mixture

Vitamin A Acetate ....................... 70 ㎍

Vitamin E ................................ 1.0 mg

Vitamin B1 ............................... 0.13 mg

Vitamin B2 .............................. 0.15 mg

Vitamin B6 ............................... 0.5 mg

Vitamin B12 ............................... 0.2 g

Vitamin C ................................. 10 mg

Biotin ................................... 10 μg

Nicotinic amide ........................... 1.7 mg

Folic acid ..................................... 50 ㎍

Calcium pantothenate ............................ 0.5 mg

Mineral mixture

Ferrous sulfate ................................ 1.75 mg

Zinc oxide .................................. 0.82 mg

Magnesium carbonate ............................. 25.3 mg

Potassium Phosphate ............................... 15 mg

Secondary calcium phosphate ............................... 55 mg

Potassium citrate ................................ 90 mg

Calcium carbonate ................................. 100 mg

Magnesium chloride .............................. 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

[Formulation Example 6] Preparation of health drink

Water scrubber extract ................. 0.0025g

Citric acid ......................... 1000 mg

Oligosaccharides ......................... 100 g

Plum concentrate ........................ 2 g

Taurine ............................ 1 g

Purified water was added to the whole to give 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 ° C for about 1 hour. The resulting solution was filtered and sterilized in a 2-liter sterile container. The resulting solution was refrigerated, Can be used for the preparation of a composition.

Although the composition ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand level, the demanding country, the use purpose, and the like. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims

Water extract ( Myriophyllum verticillatum ) extract as an active ingredient,
An antimicrobial composition having an antimicrobial activity against at least one strain selected from the group consisting of Staphylococcus sp. , Pseudomonas sp. , And Burkholderia sp .

delete

The method according to claim 1,
Wherein said strain is selected from the group consisting of Staphylococcus aureus , Staphylococcus epidermidis , opportunistic infectious pathogens Pseudomonas aeruginosa , Burkholderia cepacia and methicillin resistant star Wherein the antimicrobial composition is at least one selected from the group consisting of methicillin resistant staphylococcus aureus (MRSA).

The method according to claim 1,
Wherein the water scouring extract is extracted with at least one solvent selected from the group consisting of water and an alcohol having 1 to 6 carbon atoms.

5. The method of claim 4,
Wherein the water scouring powder extract is a methanol extract.

The method according to claim 1,
Wherein the water scouring extract comprises 0.02 to 50% by weight based on the total weight of the composition.

The method according to claim 1,
Wherein the composition is a pharmaceutical composition for preventing or treating atopic skin disease.

The method according to claim 1,
Wherein said composition is a cosmetic composition.

The method according to claim 1,
Wherein said composition is a food composition.

The method according to claim 1,
Wherein said composition is for a wet tissue, a detergent, a preservative, a disinfectant, a toothpaste or an oral cleanser.