CN104664164A - Feed additive capable of reducing white spot syndrome virus disease of penaeus vannamei boone - Google Patents
Feed additive capable of reducing white spot syndrome virus disease of penaeus vannamei boone Download PDFInfo
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Abstract
The invention discloses a feed additive capable of reducing the white spot syndrome virus disease of penaeus vannamei boone. The feed additive comprises the following components in parts by weight: 0.5-2 parts of photosynthetic bacteria, 0.2-1.5 parts of bacillus subtilis, 1-3 parts of glucose, 2-4 parts of Chinese honey locust, 3-7 parts of purple perilla, 5-7 parts of green tangerine peel, 1-4 parts of cassia twig, 3-7 parts of white paeony roots, 0.01-0.5 part of vitamin C, 0.01-0.05 part of vitamin K and 15-20 parts of a carrier. 0.1-1 percent by weight of the feed additive disclosed by the invention is added into a conventional mixed feed for a prawn, and the obtained feed can be used for feeding the penaeus vannamei boone. By the adoption of the feed additive disclosed by the invention, the occurrence of the white spot syndrome virus disease in the cultivation process of the penaeus vannamei boone can be remarkably reduced, the cultivation survival rate is improved, the quality of a prawn body is improved, the water quality is improved, and the economical and ecological benefits are increased.
Description
Technical field
The present invention relates to a kind of feed addictive used for aquiculture, particularly relate to a kind of feed addictive that can reduce Penaeus Vannmei white spot syndrome virus disease.
Background technology
Penaeus Vannmei (Penaeus vannamei Boone), originates in waters, pacific rim, America, and main distribution northern Peru is littoral to the Gulf of Mexico, the most concentrated along the distribution of Penaeus Vannmei bank with Ecuador.Penaeus Vannmei have individual large, growth is fast, nutritional need is low, premunition is strong, stronger to the adaptive capacity of water environment factors,, dressing percentage low to forage protein content requirement up to more than 65%, out-of-water survival time is long, being the improved seeds of intensive high yielding culture, is also the shrimp species that in current three large cultured prawns in the world, single rate is the highest.
The cultured output of the current Penaeus Vannmei of China has reached over half in prawn output, and along with the expanding day of intensive culture scale, the increase of cultivation density simultaneously, the deterioration of breeding environment, cause the decline of shrimp body physique, disease increase, cause serious loss to aquatic products industry.
White spot syndrome virus is the colourless liquid in cyst membrane, the infected prawn death rate is high, sick shrimp mobility declines, slowly swim at pool side or lie prostrate, do not ingest, empty stomach, slow, slow in reacting, the body colour of swimming be micro-red or normal usually dead in 3-7 days, because healthy shrimp is to the predation of sick shrimp, therefore the full pool in one week, can be caused dead.Effective methods for the treatment of be there is no at present for this disease, the comprehensive precautionary measures of main employing.
Application number is 201210192417.3, date of application 2012.06.11, name is called in ' a kind of microorganism formulation for improving north and south white shrimp immunity ' and discloses a kind of probiotics that can improve north and south white shrimp immunity, comprise bacillus subtilis, bacillus licheniformis, fermentation auxiliary material, be composited according to the parts by weight ratio of 3-8:2:1.By inoculating these two kinds high-quality effective bacterium, have good Tiny ecosystem adjustment and the effect of develop immunitypty, the raising effect for north and south white shrimp immunity is remarkable.But this invention can not play good prevention effect for white spot syndrome virus disease.
Application number is 201310293136.1, date of application 2013.07.13, name is called in ' a kind of Penaeus Vannmei feed additive special ' and discloses a kind of Penaeus Vannmei feed additive special, comprises the component of following parts by weight: purple perilla 5-10 part, Chinese honey locust 5-8 part, rascal 5-7 part, cassia twig 2-4 part, anise 2-3 part, root of herbaceous peony 3-7 part, vitamin C 0.02 part, vitamin K 0.001 part, carrier 20-25 part.The present invention obviously can improve the economic benefit of culture of Penaeus vannamei, especially the generation of disease in culture of Penaeus vannamei process can significantly be reduced, survival rate improves 19-22%, promote the growth of Penaeus Vannmei, and prawn quality can be improved, same the present invention has limitation, can not produce good prevention effect for Penaeus Vannmei guttate morphea syndrome, and easily causes water body oxygen enrichment.
Summary of the invention
In order to solve the problem, feed addictive of the present invention is intended to the generation reducing white spot syndrome virus disease in culture of Penaeus vannamei process, improves survival rate, and promotes the speed of growth of Penaeus Vannmei, improves shrimp body quality.
For achieving the above object, technical scheme of the present invention is:
A feed addictive for Penaeus Vannmei white spot syndrome virus disease can be reduced, comprise the component of following parts by weight: photosynthetic bacteria 0.5-2 part, bacillus subtilis 0.2-1.5 part, glucose 1-3 part, Chinese honey locust 2-4 part, purple perilla 3-7 part, rascal 5-7 part, cassia twig 1-4 part, root of herbaceous peony 3-7 part, vitamin C 0.01-0.5 part, vitamin K 0.01-0.05 part, carrier 15-20 part.
Preferably, a feed addictive for Penaeus Vannmei white spot syndrome virus disease can be reduced, comprise the component of following parts by weight: photosynthetic bacteria 0.9-2 part, bacillus subtilis 0.2-1.0 part, glucose 1-2 part, Chinese honey locust 3-4 part, purple perilla 3-5 part, rascal 5-6 part, cassia twig 3-4 part, root of herbaceous peony 4-7 part, vitamin C 0.05-0.5 part, vitamin K 0.03-0.05 part, carrier 18-20 parts.
Preferably, a feed addictive for Penaeus Vannmei white spot syndrome virus disease can be reduced, comprise the component of following parts by weight: photosynthetic bacteria 1.2 parts, bacillus subtilis 0.5 part, glucose 1.3 parts, 4 parts, Chinese honey locust, purple perilla 5 parts, 5 parts, rascal, cassia twig 3 parts, the root of herbaceous peony 4 parts, vitamin C 0.5 part, vitamin K 0.03 part, 19 parts, carrier.
Preferably, described photosynthetic bacteria is fisheries water dissolubility photosynthetic bacteria, photosynthetic bacteria content >=20,000,000,000/gram.
Preferably, described bacillus subtilis is aquatic products with bacillus subtilis, effective viable bacteria >=20,000,000,000/gram, enzyme activity >=2000 μ/gram.
Preferably, described carrier mixes formed mixture with zeolite powder according to 2:1 weight ratio for corn protein powder.
A kind of preparation method that can reduce the feed addictive of Penaeus Vannmei white spot syndrome virus disease is: take each raw material according to percentage by weight, first described Chinese honey locust, purple perilla, rascal, cassia twig, the root of herbaceous peony five kinds of Chinese medicines are mixed and pulverized 300-500 micron pore size sieve, then powder is mixed with taken photosynthetic bacteria, bacillus subtilis, glucose, vitamin C, vitamin K, carrier, stir and pack.
Feed addictive of the present invention is added in conventional prawn mixed feed, for the raising of Penaeus Vannmei according to percetage by weight 0.1-1%.
Propagating artificially in water environment, hydrobiological density is large, it is natural tens times to hundreds of times, the pollution that artificially applying fertilizer bait throwing in and fishes and shrimps are drained and caused is quite serious, organic substance in water increases, every physical and chemical index severe exacerbation of water quality, by manually dropping into photosynthetic bacteria in breeding water body, plays the effect of improvement water environment.
Photosynthetic bacteria preparation has many-sided comprehensive effect in aquaculture field, and Main Function has as three broad aspect such as the water conditioner improving water environment, the harmful miscellaneous bacteria inhibitor controlling pathogenic microorganism and the salubrious additive of bait of raising animal resistance.Effect of bacillus subtilis viable bacteria is: 1. the excreta that can decompose rapidly aquatic livestock, residual bait and organic dirt, eliminates water body stink, purifies water, enhance the transparency.2. can the effective noxious material such as degrading nitrite, ammonia nitrogen and hydrogen sulfide, Optimal culture environment.3. suppress the breeding of harmful algae and pathogenic microorganism, promote beneficial microorganism growth, maintain the balance of the organic substance in water.4. regulate the balance of animal gastrointestinal tract, improve the ecological environment, strengthen immunologic function, reduce the generation of disease, improve efficiency of feed utilization, reduce aquaculture cost.
Chinese honey locust (Gleditsia sinensis Lam.) has another name called honey locust, one of China distinctive Caesalpiniaceae Gleditsia seeds, Chinese honey locust water logging agent (1:3), all has inhibitory action in various degree to the dermatophyte such as trichophyton, star-shaped nocardia in vitro.Purple perilla (Perilla frutescens (L.) Britt.), another name: osmanthus is weak, common perilla, red Soviet Union etc.; For Labiatae annual herb plant.The water decoction of its leaf has inhibitory action to staphylococcus aureus, perilla leaf medicinal extract has bacteriostasis to fungi.Rascal (Green Tangerine Peel) is evergreen dungarunga or shrub, height about 3 meters; Branch is weak, usual spinosity.Contained hesperetin has very strong antiinflammatory action.Cassia twig (CassiaTwig) is the dry spray of Lauraceae aiphyllium plant Chinese cassia tree.Cassia twig alcohol extract can suppress Escherichia coli, hay bacillus and staphylococcus aureus in vitro, and valid density is 25mg/ml or following; Also inhibitory action is had to staphylococcus albus, shigella, Typhoid and paratyphoid first bacillus, pneumococcus, aerobacteria, proteus, bacillus anthracis, Salmonella enteritidis, comma bacillus etc.Cassia twig decoction also has comparatively high inhibition effect to Common Pathogenic dermatophyte.The root of herbaceous peony, also known as RADIX PAEONIAE ALBA (White Paeony Root), is Ranunculaceae Paeonia plant, has calm antiphlogistic antibacterial effect, effectively can improve shrimp body resistance.By mutually working in coordination with between above-mentioned herbal medicine, jointly promote, reach and improve immunity, enhancing development, ensure photosynthetic bacteria and bacillus subtilis activity simultaneously, common reduction Penaeus Vannmei white spot syndrome virus disease.
Advantage of the present invention is as follows:
1. feed addictive of the present invention with the addition of photosynthetic bacteria and bacillus subtilis two strain activated bacterial, significantly can reduce the generation of white spot syndrome virus disease in culture of Penaeus vannamei process, improve survival rate, improve shrimp body quality, increase economic efficiency, can purify water simultaneously, improve ecological benefits.
2. the present invention has mainly selected natural, safe medicinal herb components, by determining kind and the proportioning consumption of its optimum, namely do not affect the activity of photosynthetic bacteria and bacillus subtilis, fabulous suppression white spot syndrome virus disease can be played again, improve shrimp body immunity, prophylactic technique effect.
3. the present invention adopts usual manner to configure, and compounding method is simple, be easy to industrialization promotion uses.
Detailed description of the invention
Describe the present invention in detail below in conjunction with specific embodiment, be used for explaining the present invention in this illustrative examples of the present invention and explanation, but not as a limitation of the invention.
Embodiment one
Take the component that parts by weight are following: photosynthetic bacteria 0.5 part, bacillus subtilis 0.2 part, glucose 1 part, 2 parts, Chinese honey locust, purple perilla 3 parts, 5 parts, rascal, cassia twig 1 part, the root of herbaceous peony 3 parts, vitamin C 0.01 part, vitamin K 0.01 part, 15 parts, carrier.First Chinese honey locust, purple perilla, rascal, cassia twig, the root of herbaceous peony five kinds of Chinese medicines are mixed and pulverized 300-500 micron pore size sieve, then powder is mixed with taken photosynthetic bacteria, bacillus subtilis, glucose, vitamin C, vitamin K, carrier, stir and pack.
Embodiment two
Take the component that parts by weight are following: photosynthetic bacteria 2 parts, bacillus subtilis 1 part, glucose 2 parts, 4 parts, Chinese honey locust, purple perilla 5 parts, 6 parts, rascal, cassia twig 4 parts, the root of herbaceous peony 7 parts, vitamin C 0.5 part, vitamin K 0.05 part, 20 parts, carrier.First Chinese honey locust, purple perilla, rascal, cassia twig, the root of herbaceous peony five kinds of Chinese medicines are mixed and pulverized 300-500 micron pore size sieve, then powder is mixed with taken photosynthetic bacteria, bacillus subtilis, glucose, vitamin C, vitamin K, carrier, stir and pack.
Embodiment three
Take the component that parts by weight are following: photosynthetic bacteria 1.2 parts, bacillus subtilis 0.5 part, glucose 1.3 parts, 4 parts, Chinese honey locust, purple perilla 5 parts, 5 parts, rascal, cassia twig 3 parts, the root of herbaceous peony 4 parts, vitamin C 0.5 part, vitamin K 0.03 part, 19 parts, carrier.First Chinese honey locust, purple perilla, rascal, cassia twig, the root of herbaceous peony five kinds of Chinese medicines are mixed and pulverized 300-500 micron pore size sieve, then powder is mixed with taken photosynthetic bacteria, bacillus subtilis, glucose, vitamin C, vitamin K, carrier, stir and pack.
Embodiment four
Take the component that parts by weight are following: photosynthetic bacteria 0.9 part, bacillus subtilis 0.2 part, glucose 1 part, 3 parts, Chinese honey locust, purple perilla 3 parts, 5 parts, rascal, cassia twig 3 parts, the root of herbaceous peony 4 parts, vitamin C 0.5 part, vitamin K 0.05 part, carrier 18 part.First Chinese honey locust, purple perilla, rascal, cassia twig, the root of herbaceous peony five kinds of Chinese medicines are mixed and pulverized 300-500 micron pore size sieve, then powder is mixed with taken photosynthetic bacteria, bacillus subtilis, glucose, vitamin C, vitamin K, carrier, stir and pack.
Embodiment four
Take the component that parts by weight are following: photosynthetic bacteria 0.9 part, bacillus subtilis 0.2 part, glucose 1 part, 3 parts, Chinese honey locust, purple perilla 3 parts, 5 parts, rascal, cassia twig 3 parts, the root of herbaceous peony 4 parts, vitamin C 0.5 part, vitamin K 0.05 part, carrier 18 part.First Chinese honey locust, purple perilla, rascal, cassia twig, the root of herbaceous peony five kinds of Chinese medicines are mixed and pulverized 300-500 micron pore size sieve, then powder is mixed with taken photosynthetic bacteria, bacillus subtilis, glucose, vitamin C, vitamin K, carrier, stir and pack.
Cultivation contrast test: choose the good two kinds of feed addictives for raising southern American white prawn of commercial type and four kinds of feed addictives described in embodiment and add in penaeus vannamei boone feed to contrast and carry out contrast feeding trial, be denoted as contrast one, two groups and experiment one to four groups respectively, feed addictive addition is parts by weight 1%.
Throw something and feed every day three times, feeding volume is the 5-7% of South America hundred prawn body weight, and Feeding time is respectively 5:30,13:30,19:00, feeding volume is 30%, 20% and 50% of day feeding volume respectively, and generally timing is thrown something and fed, if Changes in weather is abnormal, during if any strong wind or heavy rain, can temporarily not throw something and feed, water degradation time, or when having prawn to raise the nose above water to breathe, can not throw, run into shrimp shelling, also do not throw, but after shelling, throw, every morning throws something and feeds more.Observe the remaining situation of feed on food platform after three hours, adjust feeding volume in time.
Cultivate from body length 3 centimetres, after 50 days feed, the growing state in the average viability of record cultivation, final average body length and breeding process, run into the dead shrimp of disease shrimp and process in time, and do Disease Analysis, concrete outcome is in table one:
Table one: Penaeus Vannmei growing state table
Test data average ± standard error represents, ρ=0.05, and when ρ < 0.05 indicates significant difference, in the long row of final body, different upper scale value representative has conspicuousness difference.
A kind of feed addictive that can reduce Penaeus Vannmei white spot syndrome virus disease of the present invention can be found out by above-mentioned experiment, can significantly reduce the generation even preventing white spot syndrome virus disease in culture of Penaeus vannamei process, the disease caused because of white spot syndrome virus is had no in breeding process, and improve survival rate, improve shrimp body quality, increase economic efficiency, there is good promotion prospect.
Above-described embodiment is illustrative principle of the present invention and effect thereof only, but not for limiting the present invention.Any person skilled in the art scholar all without prejudice under spirit of the present invention and category, can modify above-described embodiment or changes.Therefore, such as have in art usually know the knowledgeable do not depart from complete under disclosed spirit and technological thought all equivalence modify or change, must be contained by claim of the present invention.
Claims (8)
1. one kind can be reduced the feed addictive of Penaeus Vannmei white spot syndrome virus disease, it is characterized in that, comprise the component of following parts by weight: photosynthetic bacteria 0.5-2 part, bacillus subtilis 0.2-1.5 part, glucose 1-3 part, Chinese honey locust 2-4 part, purple perilla 3-7 part, rascal 5-7 part, cassia twig 1-4 part, root of herbaceous peony 3-7 part, vitamin C 0.01-0.5 part, vitamin K 0.01-0.05 part, carrier 15-20 part.
2. a kind of feed addictive reducing Penaeus Vannmei white spot syndrome virus disease according to claim 1, it is characterized in that, comprise the component of following parts by weight: photosynthetic bacteria 0.9-2 part, bacillus subtilis 0.2-1.0 part, glucose 1-2 part, Chinese honey locust 3-4 part, purple perilla 3-5 part, rascal 5-6 part, cassia twig 3-4 part, root of herbaceous peony 4-7 part, vitamin C 0.05-0.5 part, vitamin K 0.03-0.05 part, carrier 18-20 parts.
3. a kind of feed addictive reducing Penaeus Vannmei white spot syndrome virus disease according to claim 1, it is characterized in that, comprise the component of following parts by weight: photosynthetic bacteria 1.2 parts, bacillus subtilis 0.5 part, glucose 1.3 parts, 4 parts, Chinese honey locust, purple perilla 5 parts, 5 parts, rascal, cassia twig 3 parts, the root of herbaceous peony 4 parts, vitamin C 0.5 part, vitamin K 0.03 part, 19 parts, carrier.
4. according to a kind of feed addictive reducing Penaeus Vannmei white spot syndrome virus disease one of claims 1 to 3 Suo Shu, it is characterized in that: described photosynthetic bacteria is fisheries water dissolubility photosynthetic bacteria, photosynthetic bacteria content >=20,000,000,000/gram.
5. according to a kind of feed addictive reducing Penaeus Vannmei white spot syndrome virus disease one of claims 1 to 3 Suo Shu, it is characterized in that: described bacillus subtilis is aquatic products with bacillus subtilis, effective viable bacteria content >=20,000,000,000/gram, enzyme activity >=2000 μ/gram.
6. according to a kind of feed addictive reducing Penaeus Vannmei white spot syndrome virus disease one of claims 1 to 3 Suo Shu, it is characterized in that: described carrier mixes formed mixture with zeolite powder according to 2:1 weight ratio for corn protein powder.
7. the preparation method of the feed addictive that one of claims 1 to 3 is described, it is characterized in that, preparation process is: take each raw material according to percentage by weight, first described Chinese honey locust, purple perilla, rascal, cassia twig, the root of herbaceous peony five kinds of Chinese medicines are mixed and pulverized 300-500 micron pore size sieve, then powder is mixed with taken photosynthetic bacteria, bacillus subtilis, glucose, vitamin C, vitamin K, carrier, stir and pack.
8. a using method for the feed addictive that one of claims 1 to 3 is described, is characterized in that: be added in conventional prawn mixed feed by this feed addictive according to percetage by weight 0.1-1%.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104920908A (en) * | 2015-07-07 | 2015-09-23 | 卞佳林 | Small-sized shrimp feed supplement containing Chinese herbal medicinal ingredient |
| CN105495051A (en) * | 2015-12-22 | 2016-04-20 | 防城港市现代科技有限公司 | Formula of on-site prepared juvenile Penaeus vannamei starter feed |
| CN105876133A (en) * | 2016-04-13 | 2016-08-24 | 江苏星海生物科技有限公司 | Feed additive for aquatic organisms and preparation method thereof |
| CN106616030A (en) * | 2016-12-08 | 2017-05-10 | 安徽省农业科学院水产研究所 | Compound Chinese herbal medicinal preparation for preventing and curing white spot disease of procambarus clarkia and method thereof |
| CN109907162A (en) * | 2019-03-29 | 2019-06-21 | 盐城工学院 | A kind of anti-Leucoplakia feed addictive based on miR-35 and its application in aquaculture |
| CN110150504A (en) * | 2019-06-21 | 2019-08-23 | 青岛海日顺智能科技有限公司 | A kind of feed addictive promoting shrimps fast-growth |
| EP3530123A4 (en) * | 2017-12-29 | 2020-08-19 | CJ Cheiljedang Corporation | Feed composition containing bacilius subtilus strain as active ingredient for preventing or treating acute hepatopancreatic necrosis disease or white spot syndrome |
| CN112375708A (en) * | 2020-11-16 | 2021-02-19 | 上海海洋大学 | Preparation for resisting white spot syndrome virus and decapod iridovirus 1 and preparation method thereof |
| CN114766608A (en) * | 2021-12-02 | 2022-07-22 | 无锡三智生物科技有限公司 | Feed additive for preventing and controlling vibrio of small-shed shrimps and preparation method thereof |
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| CN103315151A (en) * | 2013-07-13 | 2013-09-25 | 肖玲 | Special feed additive for South American white shrimp |
| CN103583880A (en) * | 2013-10-18 | 2014-02-19 | 上海永凡生物科技有限公司 | Microbial fermentation feed for litopenaeus vannamei |
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Patent Citations (2)
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| CN103315151A (en) * | 2013-07-13 | 2013-09-25 | 肖玲 | Special feed additive for South American white shrimp |
| CN103583880A (en) * | 2013-10-18 | 2014-02-19 | 上海永凡生物科技有限公司 | Microbial fermentation feed for litopenaeus vannamei |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920908A (en) * | 2015-07-07 | 2015-09-23 | 卞佳林 | Small-sized shrimp feed supplement containing Chinese herbal medicinal ingredient |
| CN105495051A (en) * | 2015-12-22 | 2016-04-20 | 防城港市现代科技有限公司 | Formula of on-site prepared juvenile Penaeus vannamei starter feed |
| CN105876133A (en) * | 2016-04-13 | 2016-08-24 | 江苏星海生物科技有限公司 | Feed additive for aquatic organisms and preparation method thereof |
| CN106616030A (en) * | 2016-12-08 | 2017-05-10 | 安徽省农业科学院水产研究所 | Compound Chinese herbal medicinal preparation for preventing and curing white spot disease of procambarus clarkia and method thereof |
| EP3530123A4 (en) * | 2017-12-29 | 2020-08-19 | CJ Cheiljedang Corporation | Feed composition containing bacilius subtilus strain as active ingredient for preventing or treating acute hepatopancreatic necrosis disease or white spot syndrome |
| CN109907162A (en) * | 2019-03-29 | 2019-06-21 | 盐城工学院 | A kind of anti-Leucoplakia feed addictive based on miR-35 and its application in aquaculture |
| CN110150504A (en) * | 2019-06-21 | 2019-08-23 | 青岛海日顺智能科技有限公司 | A kind of feed addictive promoting shrimps fast-growth |
| CN112375708A (en) * | 2020-11-16 | 2021-02-19 | 上海海洋大学 | Preparation for resisting white spot syndrome virus and decapod iridovirus 1 and preparation method thereof |
| CN112375708B (en) * | 2020-11-16 | 2023-05-26 | 上海海洋大学 | Preparation for resisting white spot syndrome virus and Octopus iridovirus 1 and preparation method thereof |
| CN114766608A (en) * | 2021-12-02 | 2022-07-22 | 无锡三智生物科技有限公司 | Feed additive for preventing and controlling vibrio of small-shed shrimps and preparation method thereof |
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