CN105104715A - Feed preventing vibrio infection of xenocypris davidi and preparation method thereof - Google Patents
Feed preventing vibrio infection of xenocypris davidi and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a fish feed, in particular to a feed preventing vibrio infection of xenocypris davidi, and also relates to a preparation method of the mixed feed of xenocypris davidi. The feed preventing vibrio infection of xenocypris davidi comprises: by mass, 20 to 30 parts of fish meal, 8 to 15 parts of soya-bean cake, 5 to 10 parts of rapeseed meal, 6 to 12 parts of rice bran, 3 to 7 parts of hairyvein agrimony, 3 to 8 parts of cassia twig, 2 to 6 parts of perilla, 3 to 7 parts of senecio, 2 to 5 parts of calamus, 2 to 6 parts of gynura bicolor, 1 to 5 parts of prunella vulgaris, 1 to 4 parts of phellodendron, 2 to 6 parts of fish oil, 1 to 4 parts of honey, 1 to 4 parts of olive oil, and 1 to 4 parts of compound vitamin. The invention provides the feed preventing vibrio infection of xenocypris davidi according to the energy metabolism mode and nutritional requirement of the xenocypris davidi, and the feed can improve the antibiotic resistance. Moreover, the xenocypris davidi fed with the feed has low feed coefficient, high specific growth rate, and strong capability of resisting diseases.
Description
Technical field
The present invention relates to a kind of fish meal, particularly a kind of feed preventing yellow tail silver xenocypris vibrio infection; The invention still further relates to a kind of preparation method of above-mentioned yellow tail silver xenocypris mixed feed.
Background technology
The fish that yellow tail silver xenocypris (formal name used at school: Xenocyprisdavidi) is Cyprinidae Xenocypris, be the middle-size and small-size fish of growth lower floor in poisons in freshwater, body is long slightly flat, little, sharp, kiss end circle is prominent, and mouth is closely the next, in a transverse fissure, lower jaw has the cutin edge of a prosperity; Dorsal part grey, belly white, gill cover trailing edge has a lurid patch, tail fin orange colour, and raw algae by main feeding plant chip, humus and bottom, and pond culture can be thrown something and fed chaff, bran etc.
Current yellow tail silver xenocypris is in cultivating process, due to continuous fertilising, bait throwing in, the most destructible water quality of remaining excrement slag, residual bait, this just often causes yellow tail silver xenocypris to produce vibrio infection, people are when processing fish disease, often according to the method that the state of an illness adopts disinfection sanitizer to splash, but medicine more or less all can have side effects or toxicity, although the pathogen in yellow tail silver xenocypris body surface and water can be killed, also usually cause the pollution of water body, and Long-Time Service can make medicine produce accumulation, affect body health, and then affect the growth rate of yellow tail silver xenocypris.Yellow tail silver xenocypris in growth course also Chang Yinwei subalimentation cause resistance not enough, also vibrio infection is caused, affect the survival rate of yellow tail silver xenocypris, therefore, develop and a kind ofly can improve anti-vibrios ability and not only can ensure feed quality but also be beneficial to the yellow tail silver xenocypris mixed feed that yellow tail silver xenocypris grows to be the problem needing solution in current yellow tail silver xenocypris aquaculture industry badly.
Summary of the invention
An object of the present invention is to provide and a kind ofly provides a kind of feed preventing yellow tail silver xenocypris vibrio infection according to yellow tail silver xenocypris energetic supersession pattern and nutritional need, improve antibacterial resistance, after the yellow tail silver xenocypris of its feeding, yellow tail silver xenocypris feed coefficient is low, specific growth rate is high, and anti-disease ability is strong.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of feed preventing yellow tail silver xenocypris vibrio infection, comprise each component of following masses number: fish meal 20-30 part, soya-bean cake 8-15 part, dish dregs of rice 5-10 part, rice bran 6-12 part, hairyvein agrimony 3-7 part, cassia twig 3-8 part, purple perilla 2-6 part, climbing groundsel 3-7 part, calamus 2-5 part, Gynura bicolor 2-6 part, selfheal 1-5 part, golden cypress 1-4 part, fish oil 2-6 part, honey 1-4 part, olive oil 1-4 part, compound vitamin 1-4 part.
Preferably, described yellow tail silver xenocypris mixed feed, comprises each component of following masses number: fish meal 23-27 part, soya-bean cake 10-13 part, dish dregs of rice 7-8 part, rice bran 8-10 part, hairyvein agrimony 4-6 part, cassia twig 4-7 part, purple perilla 3-5 part, climbing groundsel 4-6 part, calamus 2-4 part, Gynura bicolor 3-5 part, selfheal 2-4 part, golden cypress 2-3 part, fish oil 3-5 part, honey 2-3 part, olive oil 2-3 part, compound vitamin 2-3 part.
Preferably, described yellow tail silver xenocypris mixed feed, comprises each component of following masses number: fish meal 25 parts, soya-bean cake 12 parts, the dish dregs of rice 7 parts, 9 parts, rice bran, hairyvein agrimony 5 parts, cassia twig 6 parts, purple perilla 4 parts, climbing groundsel 4 parts, calamus 3 parts, Gynura bicolor 3 parts, selfheal 2 parts, golden cypress 2 parts, 4 parts, fish oil, honey 2 parts, 2 parts, olive oil, compound vitamin 2 parts.
The present invention is the one yellow tail silver xenocypris feed targetedly developed in the abundant yellow tail silver xenocypris digestive system structure of research and unique physiological basis of feeding and nutrition, can meet the nutritional need to protein and fat of yellow tail silver xenocypris.The present invention adopts hairyvein agrimony, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, and golden cypress is can improve the anti-vibrios ability of yellow tail silver xenocypris postlarva, prevent disease.
Hot water or the ethanol immersion liquid of hairyvein agrimony all have inhibitory action to hay bacillus, staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, shigella flexneri and typhoid bacillus etc. in vitro, and the agrimophol that hairyvein agrimony contains can impel the effect of killing imago of blood fluke, in feed, add hairyvein agrimony effectively can suppress vibrios, and the parasite in fish body can be killed.Cassia twig alcohol extract can suppress Escherichia coli, hay bacillus and staphylococcus aureus in vitro, also has inhibitory action to staphylococcus albus, Shigella shigae, Typhoid and paratyphoid first bacillus, pneumococcus, aerobacteria, proteus, bacillus anthracis, Bacterium enteritidis, comma bacillus etc.In feed, add cassia twig effectively can suppress vibrios, and good inhibitory action can be had to various pathogens.The perillaldehyde of perilla leaf has good bacteriostasis, scientific experiment proves there is obvious inhibitory action to typhoid bacillus, comma bacillus, and purple perilla can promote fish enterocinesia effect, enteral prods is moved and accelerates, be conducive to the absorption of fish to feed, improve the ability of anti-disease.Climbing groundsel has stronger antibacterial action to will Hayes dysentery bar mattress and staphylococcus aureus, to bacillus such as typhoid fever, paratyphoid A, paratyphoid B, dysentery (Fu Shi, Boydii, Song Nei Shi), large intestine, distortion, cured sample anthraxs, and sarcine all has inhibitory action, in feed, add a thousand li luminous energy effectively suppress pathogenic bacteria.Calamus can stimulate gastric secretion, and has certain help to the abnormal fermentation preventing stomach and intestine, effectively can promote that fish is to the absorption of nutriment, improve the growth rate of yellow tail silver xenocypris fish.Gynura bicolor has the effect such as promoting blood circulation and hemostasis, removing toxicity for detumescence, is rich in flavonoid glycoside composition in Gynura bicolor, can extend ascorbic effect, can improve anti parasitic and antiviral ability.Selfheal decoction has inhibitory action to shigella dysenteriae, typhoid bacillus, comma bacillus, Escherichia coli, proteus, Pseudomonas aeruginosa and staphylococcus, streptococcus.Golden cypress antibiotic effective ingredient is jamaicin, the experiment proved that golden cypress all has good inhibition to staphylococcus aureus, pneumococcus, corynebacterium diphtheriae, Streptococcus viridans, shigella dysenteriae etc.Hairyvein agrimony, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, golden cypress acts synergistically each other, there is the effect of good suppression vibrios, and purple perilla, calamus, while other raw materials auxiliary play the effect of suppression vibrios, effectively promoting that fish is to the absorption of nutriment, improve the growth rate of yellow tail silver xenocypris fish.
Another object of the present invention is to provide a kind of preparation method of above-mentioned yellow tail silver xenocypris mixed feed, and the preparation method of this yellow tail silver xenocypris mixed feed comprises the following steps:
(1) respectively will purchased from market fish meal, soya-bean cake, the dish dregs of rice, rice bran, and after compound vitamin takes mixing according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(2) hairyvein agrimony is taken according to proportioning, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, golden cypress is cleaned in pure fresh water, cleaner with distilled water flushing, add after purified water is heated to boiling and continue to boil 1.5h, obtain decoction liquor, decoction liquor is positioned in the extraction kettle in supercritical extract instrument, utilize carbon dioxide to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, and separator temperature is 70 DEG C, extract 2 hours, be extracted liquid;
(3) extract step (2) obtained concentrates at outer circulation inspissator, and concentrated condition is: pressure 0.03Mpa, temperature 70 C, is concentrated into the medicinal extract that relative density is 1.13 ~ 1.14;
(4) take fish oil according to proportioning, honey, olive oil, and grounds travel, medicinal extract that above-mentioned steps obtains are put into container and are uniformly mixed and obtain object.
In preparation method, fish meal, soya-bean cake, the dish dregs of rice, rice bran, and compound vitamin is after ball milling, raw material Middle nutrition material is more easily separated out, liposoluble substance in raw material is more easily dissolved by the fish oil in subsequent step, adds the absorptivity of fish to the nutrition goods and materials of feed; By hairyvein agrimony, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, golden cypress carries out boiling can the irritation effect of feed change, relatively mildly acts on fish, and supercritical extract, concentration step retain the nutritional labeling of raw material of the present invention to greatest extent.
The advantage that mash feed directly throws something and feeds made by the present invention's yellow tail silver xenocypris mixed feed: preparation process is simple, and the feedstuff lost in preparation process is few, and the utilization rate of yellow tail silver xenocypris to feed is high.
Mixed feed of the present invention storage preservation condition: namely join namely use or, if the resting period was more than 20 days, 4 DEG C of sealings are preserved, if the resting period more than 3 months, puts ultra low temperature freezer preserve (i.e. subzero 80 degrees Celsius of preservations).
Compared with prior art, the invention has the beneficial effects as follows:
(1) yellow tail silver xenocypris likes feed of the present invention of ingesting, and feed of the present invention meets the nutritional need of yellow tail silver xenocypris; Invention adopts hairyvein agrimony, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, and golden cypress is can improve the anti-vibrios ability of yellow tail silver xenocypris postlarva, prevent disease;
Hot water or the ethanol immersion liquid of hairyvein agrimony all have inhibitory action to hay bacillus, staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, shigella flexneri and typhoid bacillus etc. in vitro, and the agrimophol that hairyvein agrimony contains can impel the effect of killing imago of blood fluke, in feed, add hairyvein agrimony effectively can suppress vibrios, and the parasite in fish body can be killed.Cassia twig alcohol extract can suppress Escherichia coli, hay bacillus and staphylococcus aureus in vitro, also has inhibitory action to staphylococcus albus, Shigella shigae, Typhoid and paratyphoid first bacillus, pneumococcus, aerobacteria, proteus, bacillus anthracis, Bacterium enteritidis, comma bacillus etc.In feed, add cassia twig effectively can suppress vibrios, and good inhibitory action can be had to various pathogens.The perillaldehyde of perilla leaf has good bacteriostasis, scientific experiment proves there is obvious inhibitory action to typhoid bacillus, comma bacillus, and purple perilla can promote fish enterocinesia effect, enteral prods is moved and accelerates, be conducive to the absorption of fish to feed, improve the ability of anti-disease.Climbing groundsel has stronger antibacterial action to will Hayes dysentery bar mattress and staphylococcus aureus, to bacillus such as typhoid fever, paratyphoid A, paratyphoid B, dysentery (Fu Shi, Boydii, Song Nei Shi), large intestine, distortion, cured sample anthraxs, and sarcine all has inhibitory action, in feed, add a thousand li luminous energy effectively suppress pathogenic bacteria.Calamus can stimulate gastric secretion, and has certain help to the abnormal fermentation preventing stomach and intestine, effectively can promote that fish is to the absorption of nutriment, improve the growth rate of yellow tail silver xenocypris fish.Gynura bicolor has the effect such as promoting blood circulation and hemostasis, removing toxicity for detumescence, is rich in flavonoid glycoside composition in Gynura bicolor, can extend ascorbic effect, can improve anti parasitic and antiviral ability.Selfheal decoction has inhibitory action to shigella dysenteriae, typhoid bacillus, comma bacillus, Escherichia coli, proteus, Pseudomonas aeruginosa and staphylococcus, streptococcus.Golden cypress antibiotic effective ingredient is jamaicin, the experiment proved that golden cypress all has good inhibition to staphylococcus aureus, pneumococcus, corynebacterium diphtheriae, Streptococcus viridans, shigella dysenteriae etc.Hairyvein agrimony, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, golden cypress acts synergistically each other, there is the effect of good suppression vibrios, and purple perilla, calamus, while other raw materials auxiliary play the effect of suppression vibrios, effectively promoting that fish is to the absorption of nutriment, improve the growth rate of yellow tail silver xenocypris fish;
(2) a kind of feed of yellow tail silver xenocypris vibrio infection each component used of preventing of the present invention is environmental protection raw material, health environment-friendly;
(3) in preparation method, fish meal, soya-bean cake, the dish dregs of rice, rice bran, and compound vitamin is after ball milling, raw material Middle nutrition material is more easily separated out, liposoluble substance in raw material is more easily dissolved by the fish oil in subsequent step, adds the absorptivity of fish to the nutrition goods and materials of feed; By hairyvein agrimony, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, golden cypress carries out boiling can the irritation effect of feed change, relatively mildly acts on fish, and supercritical extract, concentration step retain the nutritional labeling of raw material of the present invention to greatest extent;
(4) the present invention is a kind of prevents the feed of yellow tail silver xenocypris vibrio infection to make mash feed directly to throw something and feed, and preparation process is simple, and the feedstuff lost in preparation process is few, and the utilization rate of yellow tail silver xenocypris to feed is high.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
In the present invention's first detailed description of the invention, feed ingredient and preparation method thereof is as follows:
Prevent a feed for yellow tail silver xenocypris vibrio infection, be made up of each component of following masses number: fish meal 20, soya-bean cake 8 parts, the dish dregs of rice 5 parts, 6 parts, rice bran, hairyvein agrimony 3 parts, cassia twig 3 parts, purple perilla 2 parts, climbing groundsel 3 parts, calamus 2 parts, Gynura bicolor 2 parts, selfheal 1 part, golden cypress 1 part, 2 parts, fish oil, honey 1 part, 1 part, olive oil, compound vitamin 1 part.
Above-mentioned a kind of preparation method of the feed of yellow tail silver xenocypris vibrio infection that prevents is as follows:
(1) respectively will purchased from market fish meal, soya-bean cake, the dish dregs of rice, rice bran, and after compound vitamin takes mixing according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(2) hairyvein agrimony is taken according to proportioning, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, golden cypress is cleaned in pure fresh water, cleaner with distilled water flushing, add after purified water is heated to boiling and continue to boil 1.5h, obtain decoction liquor, decoction liquor is positioned in the extraction kettle in supercritical extract instrument, utilize carbon dioxide to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, and separator temperature is 70 DEG C, extract 2 hours, be extracted liquid;
(3) extract step (2) obtained concentrates at outer circulation inspissator, and concentrated condition is: pressure 0.03Mpa, temperature 70 C, is concentrated into the medicinal extract that relative density is 1.13 ~ 1.14;
(4) take fish oil according to proportioning, honey, olive oil, and grounds travel, medicinal extract that above-mentioned steps obtains are put into container and are uniformly mixed and obtain object
Embodiment 2
In the present invention's second detailed description of the invention, feed ingredient and preparation method thereof is as follows:
Prevent a feed for yellow tail silver xenocypris vibrio infection, be made up of each component of following masses number: fish meal 30 parts, soya-bean cake 15 parts, the dish dregs of rice 10 parts, 12 parts, rice bran, hairyvein agrimony 7 parts, cassia twig 8 parts, purple perilla 6 parts, climbing groundsel 7 parts, calamus 5 parts, Gynura bicolor 6 parts, selfheal 5 parts, golden cypress 4 parts, 6 parts, fish oil, honey 4 parts, 4 parts, olive oil, compound vitamin 4 parts.
The preparation method of above-mentioned yellow tail silver xenocypris mixed feed is with embodiment 1.
Embodiment 3
In the present invention's the 3rd detailed description of the invention, feed ingredient and preparation method thereof is as follows:
Prevent a feed for yellow tail silver xenocypris vibrio infection, be made up of each component of following masses number: fish meal 23 parts, soya-bean cake 10 parts, the dish dregs of rice 7 parts, 8 parts, rice bran, hairyvein agrimony 4 parts, cassia twig 4 parts, purple perilla 3 parts, climbing groundsel 4 parts, calamus 2 parts, Gynura bicolor 3 parts, selfheal 2 parts, golden cypress 2 parts, 3 parts, fish oil, honey 2 parts, 2 parts, olive oil, compound vitamin 2 parts.
The preparation method of above-mentioned yellow tail silver xenocypris mixed feed is with embodiment 1.
Embodiment 4
In the present invention's the 4th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
Prevent a feed for yellow tail silver xenocypris vibrio infection, be made up of each component of following masses number: fish meal 27 parts, soya-bean cake 13 parts, the dish dregs of rice 8 parts, 10 parts, rice bran, hairyvein agrimony 6 parts, cassia twig 7 parts, purple perilla 5 parts, climbing groundsel 6 parts, calamus 4 parts, Gynura bicolor 5 parts, selfheal 4 parts, golden cypress 3 parts, 5 parts, fish oil, honey 3 parts, 3 parts, olive oil, compound vitamin 3 parts.
The preparation method of above-mentioned yellow tail silver xenocypris mixed feed is with embodiment 1.
Embodiment 5
In the present invention's the 5th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
Prevent a feed for yellow tail silver xenocypris vibrio infection, be made up of each component of following masses number: fish meal 25 parts, soya-bean cake 12 parts, the dish dregs of rice 7 parts, 9 parts, rice bran, hairyvein agrimony 5 parts, cassia twig 6 parts, purple perilla 4 parts, climbing groundsel 4 parts, calamus 3 parts, Gynura bicolor 3 parts, selfheal 2 parts, golden cypress 2 parts, 4 parts, fish oil, honey 2 parts, 2 parts, olive oil, compound vitamin 2 parts.
The preparation method of above-mentioned yellow tail silver xenocypris mixed feed is with embodiment 1.
1, feeding experiment
Commercially available mixed feed (the commercially available mixed feed chosen in test is purchased from Shandong and rises rope fish feed research center) and the feed of embodiment of the present invention 1-embodiment 5 are carried out contrast experiment, and experimental result is as table 1-table 3:
To put into cement culturing pool with a collection of yellow tail silver xenocypris fish, each culturing pool is put into 1000 tails and is often planted feed process and establish four repetitions, and in this experimental result, data represent with mean+SD (mean ± SD).
Specific growth rate measures: measure the original body mass through the emptying yellow tail silver xenocypris fish of hunger when experiment starts, carry out daily feeding and management in experimentation, and experiment terminates the rear yellow tail silver xenocypris fish body weight measuring hungry two days.
Specific growth rate (SGR) formula of yellow tail silver xenocypris fish is:
SGR (part d
-1)=100 (lnWW2-lnWW1) T
-1;
WW1 and WW2 is respectively the initial and whole opisthosoma weight of yellow tail silver xenocypris fish in each cement culturing pool; T is experimental period (60 days).
From table 1, through 60d experiment, mixed feed of the present invention compared with existing mixed feed, the specific growth rate significant difference (p<0.05) of yellow tail silver xenocypris fish.
The specific growth rate of table 1 DIFFERENT FEED processed group yellow tail silver xenocypris fish
As table 2, mixed feed of the present invention is compared with existing mixed feed, and the survival rate of yellow tail silver xenocypris fish is without significant difference (p>0.05).
The survival rate of table 2 DIFFERENT FEED processed group yellow tail silver xenocypris fish
As table 3, mixed feed of the present invention compared with existing mixed feed, the feed coefficient significant difference (p<0.05) of yellow tail silver xenocypris fish.
The feed coefficient of table 3 DIFFERENT FEED processed group yellow tail silver xenocypris fish
2, bacteriostatic test inspection
Adopt potassium permanganate powder to contrast, embodiment 1 gained fish meal is that experimental group carries out antibacterial inspection.
The preparation of culture medium: take in the water-soluble 800mL of beef extract 5g, adds peptone 10g and dissolves, and adds casein 34g and dissolves, then adds NaCl5g dissolving, is adjusted to pH7.6 ~ 7.8, is diluted to 1000mL.Add agar powder 18g, autoclaving 15min, when being cooled to 40 DEG C ~ 50 DEG C, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, solidifies, and the 4 DEG C of refrigerators placing no bacteria pollution are for subsequent use.
Bacterial classification is: comma bacillus.
Control group antibacterial liquid is prepared: be dissolved in respectively in 10ml aseptic deionized water by potassium permanganate powder, diluted respectively by liquor strength, with stoste than being 1:2,1:4,1:8,1:16,1:32.
Experimental group antibacterial liquid is prepared: be dissolved in respectively in 10ml aseptic deionized water by fish meal leachate, diluted respectively by liquor strength, with stoste than being 1:2,1:4,1:8,1:16,1:32.Comma bacillus is antibacterial the results are shown in Table 4.
Table 4
As can be seen from table 1-table 3, use the specific growth rate of the yellow tail silver xenocypris fish of feed of the present invention to be significantly higher than the specific growth rate (p<0.05) using existing mixed feed, the growth using feed of the present invention to be more conducive to yellow tail silver xenocypris fish is described; Use the survival rate of the yellow tail silver xenocypris fish of feed of the present invention and use the survival rate of existing mixed feed without significant difference (p>0.05); Feed coefficient significantly lower than the feed coefficient (p<0.05) using existing mixed feed, illustrates that, compared with existing mixed feed, feed conversion rate of the present invention is high, result of use good.
As can be seen from Table 4, fish meal of the present invention has better bactericidal effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (4)
1. prevent a feed for yellow tail silver xenocypris vibrio infection, it is characterized in that, comprise each component of following masses number: fish meal 20-30 part, soya-bean cake 8-15 part, dish dregs of rice 5-10 part, rice bran 6-12 part, hairyvein agrimony 3-7 part, cassia twig 3-8 part, purple perilla 2-6 part, climbing groundsel 3-7 part, calamus 2-5 part, Gynura bicolor 2-6 part, selfheal 1-5 part, golden cypress 1-4 part, fish oil 2-6 part, honey 1-4 part, olive oil 1-4 part, compound vitamin 1-4 part.
2. a kind of feed preventing yellow tail silver xenocypris vibrio infection according to claim 1, is characterized in that, comprise each component of following masses number: fish meal 23-27 part, soya-bean cake 10-13 part, dish dregs of rice 7-8 part, rice bran 8-10 part, hairyvein agrimony 4-6 part, cassia twig 4-7 part, purple perilla 3-5 part, climbing groundsel 4-6 part, calamus 2-4 part, Gynura bicolor 3-5 part, selfheal 2-4 part, golden cypress 2-3 part, fish oil 3-5 part, honey 2-3 part, olive oil 2-3 part, compound vitamin 2-3 part.
3. a kind of feed preventing yellow tail silver xenocypris vibrio infection according to claim 1, is characterized in that, comprise each component of following masses number: fish meal 25 parts, soya-bean cake 12 parts, the dish dregs of rice 7 parts, 9 parts, rice bran, hairyvein agrimony 5 parts, cassia twig 6 parts, purple perilla 4 parts, climbing groundsel 4 parts, calamus 3 parts, Gynura bicolor 3 parts, selfheal 2 parts, golden cypress 2 parts, 4 parts, fish oil, honey 2 parts, 2 parts, olive oil, compound vitamin 2 parts.
4. a kind of preparation method preventing the feed of yellow tail silver xenocypris vibrio infection that claim 1-3 is described arbitrarily, is characterized in that comprising the following steps:
(1) respectively will purchased from market fish meal, soya-bean cake, the dish dregs of rice, rice bran, and after compound vitamin takes mixing according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(2) hairyvein agrimony is taken according to proportioning, cassia twig, purple perilla, climbing groundsel, calamus, Gynura bicolor, selfheal, golden cypress is cleaned in pure fresh water, cleaner with distilled water flushing, add after purified water is heated to boiling and continue to boil 1.5h, obtain decoction liquor, decoction liquor is positioned in the extraction kettle in supercritical extract instrument, utilize carbon dioxide to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, and separator temperature is 70 DEG C, extract 2 hours, be extracted liquid;
(3) extract step (2) obtained concentrates at outer circulation inspissator, and concentrated condition is: pressure 0.03Mpa, temperature 70 C, is concentrated into the medicinal extract that relative density is 1.13 ~ 1.14;
(4) take fish oil according to proportioning, honey, olive oil, and grounds travel, medicinal extract that above-mentioned steps obtains are put into container and are uniformly mixed and obtain object.
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CN105613380A (en) * | 2016-01-19 | 2016-06-01 | 周天赐 | Artificial propagation method of xenocypris davidi |
CN106562110A (en) * | 2016-11-02 | 2017-04-19 | 通化师范学院 | Chinese herbal medicinal feed additive composition for salvelinus malma and preparation method and use thereof |
CN110393239A (en) * | 2019-07-24 | 2019-11-01 | 肇庆学院 | A kind of anti-freshwater fish flavobacterium columnare feed |
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