CN102389041B - Chinese herbal feed additive for red swamp crayfish - Google Patents
Chinese herbal feed additive for red swamp crayfish Download PDFInfo
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- CN102389041B CN102389041B CN 201110341291 CN201110341291A CN102389041B CN 102389041 B CN102389041 B CN 102389041B CN 201110341291 CN201110341291 CN 201110341291 CN 201110341291 A CN201110341291 A CN 201110341291A CN 102389041 B CN102389041 B CN 102389041B
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Abstract
The invention discloses a Chinese herbal feed additive for red swamp crayfish as well as a preparation method and application thereof. The feed additive contains a water extract liquid from whole flowers of perennial semi-evergreen twinning woody climbing plant honeysuckle of the Caprifoliaceae family and oligoxylose at a mass ratio of 150:1. The preparation method comprises the steps as follows: drying by adsorbing the water extract liquid of honeysuckle whole flowers with zeolite, wherein the weight ratio of water extract liquid of honeysuckle whole flowers to zeolit is 1:6.5; after drying, mixing the water extract liquid of honeysuckle whole flowers with oligoxylose at a ratio of 150:1; and mixing to obtain the feed additive. The application of the feed additive is as follows: the feed additive is added to a red swamp crayfish feed at an additional amount of 1.5%. The Chinese herbal feed additive is low in cost and environment-friendly, can increase the utilization efficiency of nutrients in a formula feed for red swamp crayfish, and can promote growth and improve meat quality of red swamp crayfish. The preparation method can be applied to large-scale industrial production. The feed additive is a novel growth-promoting additive for red swamp crayfish formula feeds.
Description
Technical field
The present invention relates to a kind of novel Ke Shi crayfish Chinese herbal feed additive and application process thereof, belong to biological product technical field, can be used as additive and be used for Ke Shi crayfish feed.
Background technology
China's culture fishery is owing to environmental pollution increases the weight of, bacterium, virus or parasite etc. cause that acute, the subacute or chronic disease of shrimps takes place frequently, cause shrimp culture production to suffer the invasion and attack of disease, survival rate reduces, the sustainable development of serious harm culture fishery.Adopt the methods such as antibiotic or chemicals to prevent or treat, often bring the drawbacks such as pollution such as microorganism drug resistance, aquatic products medicament residue and water environment, work the mischief to animal health and production and to human health.Therefore, from natural materials, seek desirable nontoxic, noresidue, efficient, improving animal immunizing power, improving aquatic product quality, reducing the aspects such as environmental pollution and brought into play the multifunctional green feed addictive of great role and just day by day receive publicity and be applied to breeding production.At present, the shrimp of having researched and developed is a lot of with the feed addictive kind, as: active bacteria formulation (probiotics), oligosaccharides (chemical probiotic), acidulant, enzyme preparation, active peptide, Chinese herbal and crude drugs preparations etc., that these additives and active material have in animal body is antibiotic, immune, growth promotion, aid digestion, reduce contamination, and on producing, all show the effect of fine application.In culture fishery, Chinese herbal and crude drugs preparations has wide material sources as novel growth promotion immunopotentiator because of it, and is cheap at present, and low-residual should not cause pathogen to produce the advantages such as drug resistance, day by day comes into one's own in recent years.
Summary of the invention
Goal of the invention: in order to overcome the uses of medicated premix in the shrimp feed such as current anti-microbial type, growth accelerator class, the first purpose of the present invention is to provide a kind of can improve the Ke Shi crayfish to the utilization rate of artifical compound feed nutritional labeling, the Ke Shi crayfish Chinese herbal feed additive that promotion Ke Shi crayfish grows, improves meat quality and environmental protection.
The second purpose of the present invention is to provide the preparation method of said herbal medicine feed addictive.
The 3rd purpose of the present invention is to provide the application of said herbal medicine feed addictive.
Technical scheme: in order to solve above-mentioned the first purpose, the technical solution adopted in the present invention is: a kind of Ke Shi crayfish Chinese herbal feed additive, it is to extract from the perennial half evergreen winding bejuco honeysuckle of Caprifoliaceae entirely to spend water extraction liquid to be equipped with carrier and xylo-oligosaccharide take mass ratio as 150: 1 compatibilities.
It is after entirely being spent the water that adds 10 times of amounts to boil to boil 60min by honeysuckle, to filter its residue and stay its aqueous solution to form that described honeysuckle is spent water extraction liquid entirely.
Described honeysuckle spends the active ingredient of water extraction liquid that the water-soluble polysaccharide of 0.37% chlorogenic acid, 0.29% caffeic acid, 0.014% galuteolin and 1.24% is arranged entirely, and described percentage is mass percent.
Mainly contain the organic acids such as isochlorogenic acid, chlorogenic acid in the honeysuckle, its medicinal history is long, has the effects such as antibiotic, antiviral, and is all effective in cure to digestion, hematological system and reproductive system.Chlorogenic acid can cause the variation of bacterial membrane current potential, thereby changes the permeability of the inside and outside material of film, and fungistatic effect is remarkable.Studies show that chlorogenic acid is not is antipathogenic composition unique in the honeysuckle, the multiple flavone compound such as cyanidenon, Quercetin is also by the quality control standard of pharmacopeia in 2005 as honeysuckle, itself has oxidation resistance flavones, and namely they have the ability of removing infringement biomolecule free radical.Xylo-oligosaccharide is to use more Oligosaccharide Additives in herding is produced, and can not only suppress harmful bacteria in the field planting growth of enteric epithelium in animal intestinal, and the effect substrate can be provided for the growth and breeding of beneficial bacterium, improves the stability of probiotic action.Compare with other functional oligosaccharide, xylo-oligosaccharide has unique advantage: 1) substantially not by the animal body digest and decompose, can all arrive large intestine, utilize for Bifidobacterium.2) Bifidobacterium there is the high selectivity cultivation effect.3) high absolute acid stability.4) compatibility with food is good, can promote the absorption of calcium.Relevant research has been carried out with regard to the xylo-oligosaccharide that adds the varying level gradient in the feed in this laboratory.The result shows that the interpolation xylo-oligosaccharide can improve growth performance and the Activity of Digestive Enzymes in Intestine of hybridized prussian carp, and is wherein better with 0.01% adding proportion gaining effect.The present invention be water extract of flos lonicerae after the zeolite adsorption drying with the xylo-oligosaccharide compatibility, still in aquatic feeds, use first as additive.
In order to solve above-mentioned the second purpose, the technical solution adopted in the present invention is: the preparation method of above-mentioned Ke Shi crayfish Chinese herbal feed additive, honeysuckle is spent water extraction liquid zeolite adsorption, drying entirely, wherein honeysuckle entirely to spend the mass ratio of water extraction liquid and zeolite be 1: 6.5, after dry and xylo-oligosaccharide with 150: 1 compatibilities, make described feed addictive behind the mixing.
In order to solve above-mentioned the 3rd purpose, the technical solution adopted in the present invention is: the application of above-mentioned Ke Shi crayfish Chinese herbal feed additive is added on the Ke Shi crayfish with in the feed with described additive with 1.5% addition.
Beneficial effect: compared with prior art, feed addictive of the present invention has following advantage: 1) can improve shrimp PAS, alkaline phosphatase activities, improve serum hemocyanin content, improve the body non-specific immunity; 2) can improve shrimp enteron aisle digestive ferment such as protease, amylase and lipase active, but the efficiency of feed utilization of Effective Raise aquatic animal; 3) be conducive to the profitable strain such as bacillus and in the shrimp alimentary canal, sprout as soon as possible, breed, form dominant microflora, and then improve the utilization ratio to feed, promote it to the digesting and assimilating of nutriment, improve the intestinal microecology of shrimp, the promotion health growth; 4) do not produce any interior exotoxin, can improve shrimp body meat quality.The preparation method of this feed addictive is easy, can be used for technical scale production.Described additive is added on the Ke Shi crayfish with in the feed with 1.5% addition, so that use safety, has no side effect, drug residue free, pollution-free, be a kind of application process that very high ecological environmental protection is worth that has.
The specific embodiment
Further set forth beneficial effect of the present invention below by test.
1, Chinese herbal feed additive is on the impact of Ke Shi crayfish
Ke Shi crayfish basal diet sees Table 1, adds respectively 0.01% xylo-oligosaccharide and 1.5% Chinese herb feed additive in Ke Shi crayfish basal diet.It is evenly mixed step by step that each organizes feed, and poach is used the small meat mincer granulation again, puts into-20 ℃ of refrigerators after naturally drying and is in store for.The test daily ration of throwing something and feeding behind the Ke Shi crayfish domestication 7d is thrown something and fed with 2%~4% of shrimp body weight.When treating formally to throw something and feed test 30d, carry out growth performance mensuration in mid-term, the mensuration body is grown and is weighed in.Test 60d, the test shrimp is carried out the mensuration of growth performance, sero-immunity index and anti-shrimp white spot syndrome virus (WSSV) ability.Evaluate this additive to the impact of Ke Shi crayfish growth performance and immunocompetence according to above index comprehensive.The result shows that this additive of interpolation 1.5% has significantly improved the rate of body weight gain of shrimp in Ke Shi crayfish daily ration, has strengthened immune indexes activity in the shrimp serum, and Ke Shi crayfish anti-virus ability strengthens, and survival rate improves.Test data sees Table 2,3 and 4.
Table 1 Ke Shi crayfish basal diet prescription and nutritional labeling (%)
1Contain vitamin in every kilogram of premix: vitamin A 900000IU, calciferol 00000IU, vitamin E 4 500mg, vitamin K
3220mg, Cobastab
1320mg, Cobastab
21090mg, Cobastab
52000mg, Cobastab
6500mg, Cobastab
121.6mg, vitamin C 5000mg, pantothenic acid 1000mg, folic acid 165mg, choline 60000mg.Contain mineral matter in every kilogram of premix: copper sulphate 2.0g, ferric sulfate 25g, zinc sulfate 22g, manganese sulfate 7g, sodium selenite 0.04g, KI 0.026g, cobalt chloride 0.1g.
Table 2 additive is on the impact of Ke Shi crayfish production performance
Table 3 additive is on the impact of Ke Shi crayfish sero-immunity index
Table 4 additive is on the impact of Ke Shi crayfish resistance against diseases
2, Chinese herbal feed additive is on the impact of pond culture Ke Shi crayfish growth performance
This test is carried out the pond culture pilot plant test on the desk research basis.Ke Shi crayfish production ration prescription sees Table 5, adds respectively 0.01% xylo-oligosaccharide, this additive of 0% and 1.5% in Ke Shi crayfish basal diet.Formal test is carried out in cultivation base, Xuyi outdoor ponds, every group of 4 repetitions.After feeding 60 days, weigh, and calculate last heavy.The result shows, adds this additive of 1.5% in artifical compound feed, and the weight in latter stage of Ke Shi crayfish is higher than not interpolation group.Test data sees Table 6.
Table 5 Ke Shi crayfish production ration prescription and nutritional labeling (%)
1Contain vitamin in every kilogram of premix: vitamin A 900000IU, calciferol 00000IU, vitamin E 4 500mg, vitamin K
3220mg, Cobastab
1320mg, Cobastab
21090mg, Cobastab
52000mg, Cobastab
6500mg, Cobastab
121.6mg, vitamin C 5000mg, pantothenic acid 1000mg, folic acid 165mg, choline 60000mg.Contain mineral matter in every kilogram of premix: copper sulphate 2.0g, ferric sulfate 25g, zinc sulfate 22g, manganese sulfate 7g, sodium selenite 0.04g, KI 0.026g, cobalt chloride 0.1g.
Table 6 additive is on the impact of Ke Shi crayfish growth performance
Conclusion
Above result shows, adds the growth performance that this additive can significantly improve shrimp in Ke Shi crayfish artifical compound feed, and strengthens shrimp body anti-virus ability, has embodied preferably immune protective effect.This additive is safe, harmless, adds cost to be
6 yuan in feed per ton, feasible on producing, when fishes and shrimps are sick, can reduce dosage and drug cost, for production of fodder person and raiser increase economic efficiency, be conducive to fisheries sustainable development, the social benefit of generation.Achievement in research has reached in several the Feed Enterprise such as feed corporation,Ltd, Xuyi Jiangsu Azolla imbricata lobster industrial park Co., Ltd and the breeding enterprise at the Xuyi Jiangsu Kingcon to be used, and has obtained larger economic benefit, social benefit and environmental effect.
The present invention is further described below by specific embodiment; should be pointed out that for the person of ordinary skill of the art, under the prerequisite that does not break away from the principle of the invention; can also make some modification and improvement, these also should be considered as belonging to protection scope of the present invention.
Embodiment: a kind of Ke Shi crayfish Chinese herbal feed additive, it is to extract from the perennial half evergreen winding bejuco honeysuckle of Caprifoliaceae entirely to spend water extraction liquid to be equipped with carrier and xylo-oligosaccharide take mass ratio as 150: 1 compatibilities.Wherein carrier is zeolite; It is after entirely being spent the water that adds 10 times of amounts to boil to boil 60min by honeysuckle, to filter its residue and stay its aqueous solution to form that honeysuckle is spent water extraction liquid entirely; Honeysuckle spends the active ingredient of water extraction liquid that the water-soluble polysaccharide of 0.37% chlorogenic acid, 0.29% caffeic acid, 0.014% galuteolin and 1.24% is arranged entirely, and described percentage is mass percent.
The preparation method of above-mentioned Ke Shi crayfish Chinese herbal feed additive, specific as follows:
Entirely spend honeysuckle water extraction liquid with zeolite adsorption, drying, wherein honeysuckle entirely to spend the mass ratio of water extraction liquid and zeolite be 1: 6.5, afterwards dry and xylo-oligosaccharide is made described feed addictive with 150: 1 compatibilities behind the mixing.
Above-mentioned additive is added on the Ke Shi crayfish with in the feed with 1.5% addition.
Claims (4)
1. Ke Shi crayfish Chinese herbal feed additive, it is characterized in that, it is that extraction is spent water extraction liquid zeolite adsorption, drying entirely from the perennial half evergreen winding bejuco honeysuckle of Caprifoliaceae, wherein honeysuckle entirely to spend the mass ratio of water extraction liquid and zeolite be 1:6.5, after dry and xylo-oligosaccharide with mass ratio 150:1 compatibility, make described feed addictive behind the mixing; It is after entirely being spent the water that adds 10 times of amounts to boil to boil 60min by honeysuckle, to filter its residue and stay its aqueous solution to form that wherein said honeysuckle is spent water extraction liquid entirely.
2. Ke Shi crayfish Chinese herbal feed additive according to claim 1, it is characterized in that, described honeysuckle spends the active ingredient of water extraction liquid that the water-soluble polysaccharide of 0.37% chlorogenic acid, 0.29% caffeic acid, 0.014% galuteolin and 1.24% is arranged entirely, and described percentage is mass percent.
3. the preparation method of described Ke Shi crayfish Chinese herbal feed additive according to claim 1, it is characterized in that, honeysuckle is spent water extraction liquid zeolite adsorption, drying entirely, wherein honeysuckle entirely to spend the mass ratio of water extraction liquid and zeolite be 1:6.5, after dry and xylo-oligosaccharide with the 150:1 compatibility, make described feed addictive behind the mixing, it is after entirely being spent the water that adds 10 times of amounts to boil to boil 60min by honeysuckle, to filter its residue and stay its aqueous solution to form that wherein said honeysuckle is spent water extraction liquid entirely.
4. the described Ke Shi crayfish of claim 1 Chinese herbal feed additive, is characterized in that with the application in the feed preparation Ke Shi crayfish, and described additive is added on the Ke Shi crayfish with in the feed with 1.5% addition, namely makes Ke Shi crayfish feed.
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CN102885235A (en) * | 2012-10-25 | 2013-01-23 | 常熟理工学院 | Procambarus clarkia feed additive as well as preparation method and use thereof |
CN103141701B (en) * | 2013-03-27 | 2014-05-07 | 南京农业大学 | Micro-ecological feed capable of improving intestinal function of pelteobagrus fulvidraco fries in feeding habit conversion period |
CN104431501A (en) * | 2014-12-01 | 2015-03-25 | 苏州市相城区盛胡特种养殖专业合作社 | Compound feed with activity of inhibiting in-vivo pathogenic bacteria of procambarus clarkii |
CN105055392A (en) * | 2015-09-14 | 2015-11-18 | 四川九章生物科技有限公司 | Application of chlorogenic acid in preparation of medicament for treating muscular dystrophy disease |
CN107874024A (en) * | 2017-11-06 | 2018-04-06 | 明光市永言水产(集团)有限公司 | A kind of lobster breeding period special feed and preparation method thereof |
CN111642655A (en) * | 2020-06-22 | 2020-09-11 | 江苏海洋大学 | Method for developing compound Chinese herbal medicine feed additive for procambarus clarkia |
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CN1078354A (en) * | 1992-05-12 | 1993-11-17 | 王葆刚 | A kind of fodder additive for raising fish and shrimp and production method thereof |
CN1264414C (en) * | 2004-12-10 | 2006-07-19 | 中国海洋大学 | Compound immunopotentiator for prawn breeding |
CN101756063A (en) * | 2008-11-04 | 2010-06-30 | 洪福山 | Feed additive capable of improving growth environment of cultured fish and shrimp |
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