CN108498583A - A kind of composition and application method of prevention Penaeus Vannmei bacteriosis - Google Patents
A kind of composition and application method of prevention Penaeus Vannmei bacteriosis Download PDFInfo
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- CN108498583A CN108498583A CN201810528129.8A CN201810528129A CN108498583A CN 108498583 A CN108498583 A CN 108498583A CN 201810528129 A CN201810528129 A CN 201810528129A CN 108498583 A CN108498583 A CN 108498583A
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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Abstract
The present invention relates to a kind of composition and application method of prevention Penaeus Vannmei bacteriosis, ingredient and volume ratio in the composition are edible oil：Eucalyptus leaf essential oil：Herba Origani Vulgaris quintessence oil：Thyme essential oil=90：5：3：2.The application method of the composition is to take 200 milliliters of water, is put into 0.9~1.2 gram of guar gum and uniformly mixes, and instills 3~5 milliliters of above-mentioned composition, while uniform stirring, admixes 1 kilogram of feed, 20 60min are dried after stirring evenly, are then put into breeding water body again.The above method can be such that composition is attached on feed, and will not be spread in the short time after entering breeding water body, it is ensured that the using effect of composition.The cultured prawn that the present composition falls ill for early stage, cure rate reach 90% or more, breach " stealing dead disease " and are difficult to the cultivation bottleneck cured；Incidence can be effectively reduced after popularization and application, it is nontoxic, it avoids using chemicals, bio-hormone and antibiotic etc. in the breeding process.
Description
Technical field
The invention belongs to aquaculture disease therapeuticing medicine fields, are prevented more particularly to a kind of Penaeus Vannmei bacillary
The composition and application method of disease.
Background technology
Penaeus Vannmei, scientific name litopenaeus vannamei are currently the highest crustacean culture kind of cultured output in the world, it
Have the characteristics that the speed of growth is fast, disease resistance is strong, dressing percentage is high, there is high culturing economic to be worth.Penaeus Vannmei in
It introduces within 1988 and spreads the whole nation behind China rapidly.Since two thousand one, the cultured area of China's litopenaeus vannamei and yield is always
Occupy the first place of prawn culturing.1990-2010, world's culture of Penaeus vannamei yield rise to 2,700,000 tons by 100,000 tons, year
Increase up to 135%.But from 2010, during culture of Penaeus vannamei, disease occurs frequently, and survival rate is low, even
There is total crop failure phenomenon, serious economic loss is caused to cultivation.Wherein, prawn Acute Hepatic pancreatic necrosis (AHPND), also known as
" stealing dead disease ", is a kind of new Major Diseases.The disease lethality is high, according to statistics, prawn culturing main producing region yield in 2011
Reduce it is inestimable to loss caused by China's shrimp culture industry between about 80%, 2011-2013, every year because of AHPND diseases
The underproduction causes profit to reduce by 1,000,000,000 dollars.In AHPND pathogeny research methods, Lightner DV in 2013 etc. are reported and are confirmed
The pathogenic original of AHPND is AHPND vibrio parahaemolytious (Vibrio parahaemolyticus).Since then, vibrio infection becomes mainstream
Theory.He Jianguo etc. is research shows that AHPND is caused by the comprehensive bacterium such as vibrio parahaemolytious, vibrio alginolyticus.According to Jiang Yan, certainly
It repeatedly goes to more to suffer from AHPND culture of Penaeus vannamei field between 2012-2016 and carries out epidemiology and Study on etiology, find
The cause of disease of the disease has diversity, filters out 6 plants of pathogenic bacterias altogether：Vibrio parahaemolytious (Vibrio
Parahaemolyticus), Aeromonas hydrophila (Aeromonas hydrophila), Vibrio harveyi (Vibrio
Harveyi), Vibrio.sp.Ex-25, Maxwell vibrios (Vibrio metschnikovii), vibrio alginolyticus (Vibrio
alginolyticus).Since China coastline is very long, breeding environment is complicated, the pathogenic original of the disease has the characteristics that multifarious.
AHPND is using fulminant Hepatopancreatic necrosis as the infectious disease of main feature, and illness prawn vigor weakens, body colour is rubescent, shell is soft, jejunum
Empty stomach, hepatopancrease become bronzing or atrophy occurs in the hepatopancrease of pale, enlargement, a small number of illness prawns.AHPND morbidities are fast
Speed, the death rate and row's pool rate are high, and the death rate is up to 90% or more within 4~5 days.Since AHPND stadium is short, lethality is high, using normal
Advise medication effect unobvious.There is presently no effective therapies, mainly to use broad-spectrum antibiotics to carry out
Prevent, control the amount of vibrio in cultivation water environment, reduces cultured prawn illness probability.Furthermore, it has been reported that some Chinese medicines can be with
Penaeus Vannmei immunity of organisms is significantly improved, there is effect to the prevention of AHPND, main includes suitably using allicin, big
The yellow, coptis and Radix Isatidis etc..According to Jiang Yan, the composite Chinese herbal garden burnet of suitable concentration, myrobalan, the coptis and dark plum compound pair
The AHPND pathogens of Penaeus Vannmei have notable fungistatic effect.
Invention content
The technical problem to be solved in the present invention is to provide a kind of natural herbal essence of prevention Penaeus Vannmei " stealing dead disease "
Oil, including several essential oils extract respectively after mixed essential oil and several different materials, such as source be added to by ratio formula
Different Chinese herbal medicine mixing, synchronous extraction are obtained containing different amounts of mixed essential oil.The essential oil solves Chinese medicine in aquatic products
On be not easy to absorb and problem inconvenient for use, there is significant antibacterial, bactericidal effect, prawn body small toxicity is easy to operate, and builds
Integrated therapeutics supporting technology and the operating specifications such as essential oil manufacture craft, the feeding embedded with adhesive are found.
The present invention is achieved by the following technical solution：
A kind of composition of prevention Penaeus Vannmei bacteriosis, ingredient and volume ratio in the composition are edible
Oil：Eucalyptus leaf essential oil：Herba Origani Vulgaris quintessence oil：Thyme essential oil=90：5：3：2.
Preferably, mixed essential oil is formulated by following raw material by percent by volume：Contain food in per 100ml compositions
With oily 90 milliliters, 5 milliliters of eucalyptus leaf essential oil, 3 milliliters of Herba Origani Vulgaris quintessence oil, 2 milliliters of thyme essential oil.
The application method of above-mentioned composition is to take 200 milliliters of water, is put into 0.9~1.2 gram of guar gum and uniformly mixes, and is instilled
3~5 milliliters of above-mentioned composition, while uniform stirring, admix 1 kilogram of feed, and 20-60min is dried after stirring evenly, is then thrown again
Enter in breeding water body.
The above method can be such that composition is attached on feed, and will not be spread in the short time after entering breeding water body,
Ensure the using effect of composition.
The advantageous effect of the present invention compared with prior art：
The present composition is natural component, prevents and inhibits disease, reduce pathogen levels, reduce the disease of aquatile
Sick incidence, improve host organism to pathogenic bacteria immunocompetence.Then the technology is done by being essential oil by traditional Chinese medicine ingredients extraction
At compound prescription solve the problems, such as effective component of chinese medicine can not direct quantitative use and it is inconvenient for use, such as usual Chinese medicine preparation need
The programs such as impregnate, heat, boiling, filtering；And the feed mode embedded with adhesive is established, which reduces conventional medicine
Be dissolved in water be directly sprayed on feed surface due to caused by drug dispersion drug effect reduce problem, and essential oil mixing compound suppression
Bacterium effect is significantly better than folk prescription.Compound prescription can improve the palatability and attractant of feed simultaneously, play raising cultivation life
The effect of the survival rate and growth rate of object.
The present invention carries out culture experiment by the cultured prawn for early stage morbidity, and cure rate reaches 90% or more.Cause
This, the present invention breaches " stealing dead disease " and is difficult to the cultivation bottleneck cured；Incidence, nontoxic nothing can be effectively reduced after popularization and application
Evil avoids using chemicals, bio-hormone and antibiotic etc. in the breeding process.
Specific implementation mode
Embodiment 1
Select inhibition zone method and micro doubling dilution from 30 kinds of broad-spectrum antibacterial Chinese herbal medicines to suffering from Acute Hepatic pancreatic necrosis
It detached in the hepatopancrease of Penaeus Vannmei, purify important pathogenic bacteria：Vibrios class (Vibrio Parahaeolyticus,
V.alginolyticus, V.harveyi, V.marinus, V.anguillarum), Aeromonas class (Aeromonas
Hydrophila, A.formicans) and pseudomonad class (Pseudomonas fluorescene, P.putida), carry out body
Outer fungistatic effect experiment, the results show that in terms of inhibition zone size, average Mlc and average bacteriocidal concentration value, eucalyptus leaves,
Wild marjoram, the fungistatic effect of three taste folk prescription Chinese herbal medicine of thyme are best, and three's optimum volume ratio is 5：3：2.
Using eucalyptus leaves, wild marjoram, three taste Chinese medicine of thyme, take hydrodistillation extract respectively eucalyptus leaf essential oil, Herba Origani Vulgaris quintessence oil,
Thyme essential oil, and the eucalyptus leaf essential oil to extracting, Herba Origani Vulgaris quintessence oil, thyme essential oil folk prescription and compound are carried out using inhibition zone method
To the bacteriostatic experiment of Penaeus Vannmei AHPND vibrio parahaemolytious (Vibrio parahaemolyticus), result of study card
Bright, the compound of three kinds of essential oils is significantly better than the compound of folk prescription and two kinds of essential oils, and the compound antibacterial circle diameter of three kinds of essential oils is maximum
Reach 25.2mm (experimental result is as shown in table 1).
Choose temporarily support 10 days after from a pair of of Penaeus Vannmei parent shrimp 400 tail of offspring (body surface flushes without wound,
Average weight is 5.0g or so), 8 groups are randomly divided into, 50 tails/group.Safety, the growth performance etc. of litopenaeus vannamei are ground
Study carefully.By folk prescription or compound essential oil with edible soybean oil according to 1：10 volume ratio is uniformly mixed.Then it is evenly mixed in feed
Surface, per kilogram feed takes 200 milliliters of sterile waters to be put into 0.8g guar gums and uniformly mixes, and instills 4 milli of above-mentioned essential oil
It rises, stirs evenly simultaneously.The feed surface of sprinkling is uniformly mixed with hand, makes its bonding on the surface of feed.It dries in air
30 minutes, then normally put into experiment training shrimp bucket.During experiment, daily 08:00、20:00 to feed spice respectively primary, and day throws
The amount of feeding accounts for 10% or so of prawn weight.The habit hidden by day and come out at night according to prawn, daily 08:00 to change water primary, is taken the photograph according to prawn
Food situation and water body cloudy state change water, day quantity of exchanged water be 10%~20%.Inhale bottom be operated in change water before carry out.Breeding water is
Natural sea-water, water temperature are 25~30 DEG C, salinity 30,5~6mg/L of dissolved oxygen, ammonia nitrogen<0.5mg/l, nitrite nitrogen<0.1mg/
l.It is observed continuously after administration, the growth conditions such as records prawn body colour daily, ingest, move about.After cultivation in 30 days, different groups
(wherein opposite average growth rate is the every of every group of experiment after cultivation 30 days as shown in table 1 for the growth of litopenaeus vannamei, survival condition
The average weight of tail shrimp subtracts the average weight of every tail shrimp when every group of experiment starts, then divided by every tail of every group of experiment when starting
The average weight of shrimp).It determines that the compound is safe and non-toxic, and the effect of the survival rate and growth rate of Penaeus Vannmei can be improved.
1 essential oil of table is to the biocidal property of litopenaeus vannamei, safety, growth performance test result
Embodiment 2
A kind of composition of prevention Penaeus Vannmei white spot syndrome virus, including eucalyptus leaf essential oil, in Herba Origani Vulgaris quintessence oil and hundred
Essential oil, percent by volume ratio are edible soybean oils：Eucalyptus leaf essential oil：Herba Origani Vulgaris quintessence oil：Thyme essential oil=90：5：3：2.
Originally it is that embodiment uses preferred technical solution, mixed essential oil to press per 100ml：90 milliliters of soybean oil, eucalyptus leaf essential oil 5
Milliliter, 3 milliliters of Herba Origani Vulgaris quintessence oil, 2 milliliters of thyme essential oil.
The application method of above-mentioned composition is to take 200 milliliters of water, is put into 1.2 grams of guar gums and uniformly mixes, and is instilled above-mentioned mixed
5 milliliters of essential oil, while uniform stirring are closed, 1 kilogram of feed is admixed, 50min is dried after stirring evenly, then puts into breeding water body again
In.
The above method can be such that composition is attached on feed, and will not be spread in the short time after entering breeding water body,
Ensure the using effect of composition.
Application examples
Clinical prevention pilot scale successively is carried out in 30 Yu Jia culture of Penaeus vannamei enterprises, clinical application is shown：In disease
Preventive effect during prevalence is good, prevents breaking out for disease substantially；For the cultivation unit of early stage morbidity, cure rate reaches
90% or more, prawn culturing survival rate significantly improves.Wherein, single batch of 1000kg/ mus of yield of feed coefficient or more, traditional outdoor ponds
500kg/ mus of per unit area yield or more, single batch of yield 10kg/m of indoor industrially3More than.The set Prevention Technique method is highly practical, clinical
Control effect is good, breaches " stealing dead disease " and is difficult to the bottleneck cured；Incidence, clinical cure rate can be effectively reduced after popularization and application
It is high.
Claims (3)
1. a kind of composition of prevention Penaeus Vannmei bacteriosis, it is characterised in that the ingredient in the composition and volume
Than for edible oil：Eucalyptus leaf essential oil：Herba Origani Vulgaris quintessence oil：Thyme essential oil=90：5：3：2.
2. a kind of composition of prevention Penaeus Vannmei bacteriosis according to claim 1, it is characterised in that described
Composition is formulated by following raw material：Contain edible oil 90ml, eucalyptus leaf essential oil 5ml, Herba Origani Vulgaris quintessence oil in per 100ml compositions
3ml, thyme essential oil 2ml.
3. the application method of composition described in claims 1 or 2 is, it is characterised in that the method is specially：200ml water is taken,
It is put into 0.9~1.2 gram of guar gum uniformly to mix, instills 3~5ml of above-mentioned composition, while uniform stirring, admix 1 kilogram of feed,
20-60min is dried after stirring evenly, and is then put into breeding water body again.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810528129.8A CN108498583B (en) | 2018-05-29 | 2018-05-29 | Composition for preventing and treating bacterial diseases of penaeus vannamei boone and use method thereof |
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| CN201810528129.8A CN108498583B (en) | 2018-05-29 | 2018-05-29 | Composition for preventing and treating bacterial diseases of penaeus vannamei boone and use method thereof |
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| CN108498583B CN108498583B (en) | 2020-12-15 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583903A (en) * | 2019-09-20 | 2019-12-20 | 山东龙昌动物保健品有限公司 | Application of eucalyptus multicupillata essential oil and compound microbial inoculum thereof in preparation of crustacean insect repellents |
| CN113040073A (en) * | 2021-03-15 | 2021-06-29 | 珠海市海盟水产养殖应用研发有限公司 | Insect-repellent essential oil complexing agent for fish and shrimp and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104906179A (en) * | 2015-04-01 | 2015-09-16 | 湖南泰谷生物兽药有限公司 | Origanum oil suspension and preparation method thereof |
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2018
- 2018-05-29 CN CN201810528129.8A patent/CN108498583B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104906179A (en) * | 2015-04-01 | 2015-09-16 | 湖南泰谷生物兽药有限公司 | Origanum oil suspension and preparation method thereof |
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| 姬广闻等: "《新编鱼病综合防治手册》", 30 April 2002, 中原农民出版社 * |
| 孙克年: "牛至及牛至精油在水产养殖中的应用研究", 《广东饲料》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583903A (en) * | 2019-09-20 | 2019-12-20 | 山东龙昌动物保健品有限公司 | Application of eucalyptus multicupillata essential oil and compound microbial inoculum thereof in preparation of crustacean insect repellents |
| CN110583903B (en) * | 2019-09-20 | 2022-08-30 | 山东龙昌动物保健品有限公司 | Application of eucalyptus multicupillata essential oil and compound microbial inoculum thereof in preparation of crustacean insect repellents |
| CN113040073A (en) * | 2021-03-15 | 2021-06-29 | 珠海市海盟水产养殖应用研发有限公司 | Insect-repellent essential oil complexing agent for fish and shrimp and application thereof |
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