CN103355533B - Composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer and its use method - Google Patents
Composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer and its use method Download PDFInfo
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- CN103355533B CN103355533B CN201310305062.9A CN201310305062A CN103355533B CN 103355533 B CN103355533 B CN 103355533B CN 201310305062 A CN201310305062 A CN 201310305062A CN 103355533 B CN103355533 B CN 103355533B
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Abstract
In order to solve the survival possibility of probiotics in high-temperature and high humidity environment and add them into aquatic microcapsule baits to produce probiotic-containing microcapsule baits suitable for shrimps, crabs, fry and the like, the invention discloses a composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer and its use method. The composite immunopotentiator mainly comprises the following raw materials by mass: 20-40% of a dried ginger extract, 5-15% of propylgallate, 25-40% of tryptophan, 6-17% of allicin, 5-20% of vitamin E, and 2-10% of ethoxy quinoline. The composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer can be blended into forage as a forage additive, and during feeding, its adding amount accounts for 0.1-0.5% of the mass of the forage; or the composite immunopotentiator can be splashed in a fish pond, and during splashing into the fish pond, the use concentration of the composite immunopotentiator is 0.2-0.4ppm. With the composite immunopotentiator provided in the invention, the Mylopharyngodon piceus's body immune function can be effectively improved, and the death rate of Mylopharyngodon piceus can be reduced.
Description
Technical field
The present invention relates to a kind of composite immune reinforcing agent, belong to technical field of aquaculture, particularly relate to a kind of composite immune reinforcing agent and the using method thereof that can prevent and treat the death of black carp summer high temperature.
Background technology
Black carp (Mylopharyngodon piceus), has another name called blue or green grass carp, blue, spiral shell green grass or young crops, black carp, black grass carp etc.Mainly be distributed in the plains region of drought, for one of CHINESE FRESHWATER cultivation " four large Chinese carps ", in China, there is long cultivation historical.2011, Chinese black carp output added up 46.77 ten thousand tons, was the important economic freshwater fish in Yangtze River Delta Area.Nutritional need for black carp, existing a lot of experts have carried out system research, also developed the special-purpose black carp feed of part, as Chinese patent application CN201110308066.3 (a kind of feed formula for black carp), Chinese patent application CN1692796 (a kind of health-care black carp feed) and Chinese patent application CN102771671A (a kind of efficient and environment-friendly type black carp expanding compound feed and production method thereof) etc.Yet along with the fast development of black carp intensive culture, we find in recent years, during the black carp fishing breeding pond summer high temperature of Yangtze River Delta Area, the death rate obviously increases.According to Relevant Analysis, this sick occurrence cause is that high-grade feed nutrition concentration is higher, and the supplementary deficiency such as vitamin, thereby cause black carp anti-stress ability under the too fast speed of growth to weaken relevant.At present, about the method for preventing and treating of this disease, mostly being at antibiotic medicines such as the chemosterilants such as water splashing PVP-I or oral Enrofloxacins, there is risk and the secondary infringement to cultivation fish of medicament residue in these methods.Therefore, develop the immunity that a kind of composite immune reinforcing agent improves black carp self, effectively reduce the death that black carp causes because of point-like aerogenesis monad during summer high temperature simultaneously, the healthy growth tool of black carp is of great significance.
Summary of the invention
The object of the invention is to, a kind of immunopotentiator that can prevent and treat the death of black carp summer high temperature and preparation method thereof is provided, when improving the black carp general level of the health, can significantly reduce the generation of the diseases such as black carp enteritis in summer, or when enteritis disease occurs, effectively alleviate disease symptoms, reduce the death rate of black carp.
In order to achieve the above object, according to the present invention, a kind of immunopotentiator of preventing and treating the death of black carp summer high temperature is proposed, it is characterized in that its raw material mainly comprises the each component of following mass percentage content: Rhizoma Zingiberis extract 20-40%, n-propyl gallate 5-15%, tryptophan 25-40%, allicin 6-17%, vitamin E 5-20%, ethoxyquinoline 2-10%.
The optimal case of composition of raw materials is: Rhizoma Zingiberis extract 30%, n-propyl gallate 15%, tryptophan 25%, allicin 10%, vitamin E 10%, ethoxyquinoline 10%.
The preparation method of Rhizoma Zingiberis extract of the present invention is: get rhizoma zingiberis and dry under 80-90 ℃ of condition, after the rhizoma zingiberis after drying is pulverized, be Rhizoma Zingiberis extract.
The using method of the immunopotentiator of control black carp summer high temperature death: as feed addictive, be stirred in feed, while throwing something and feeding, the 0.1-0.5% that its addition is feeding quality; Or splash in fishpond, while splashing in fish pond, its working concentration is 0.2-0.4ppm.
In raw material: rhizoma zingiberis warming spleen and stomach for dispelling cold, strong fortune spleen sun, in Chinese medicine, be used as and control deficiency-cold in spleen and stomach, coldness and pain in the epigastrium, vomiting is had loose bowels; N-propyl gallate is a kind of fat-soluble food antioxidant, usually used as food additives, for preventing Food Oxidation and rotten.Tryptophan is a kind of important nutritional agents, can participate in the renewal of plasma proteins in animal body, and can impel riboflavin to play a role, and also contributes to the synthetic of nicotinic acid and ferroheme, can significantly increase the antibody in animal body, improves the immunity of animal body.Allicin has stronger antibacterial and anti-inflammation functions, and multiple coccus, bacillus, fungi, virus etc. are all had and suppressed or killing action.Vitamin E is one of topmost antioxidant, and the injury that protection skin is avoided ultraviolet ray and polluted, makes slightly blood vessel dilatation of end, improves blood circulation, can effectively improve body endurance, strengthen immunologic function.Ethoxyquinoline is one of feed antioxidant of function admirable, it is most economical antioxidant, be applicable to premix, fish meal and add fatty product, can prevent that vitamin A. D. E wherein etc. and fatty oxygen from dividing rotten and natural colouring matter oxidation stain, and have certain mildew-resistant and preservation.
The composite immune reinforcing agent of the control black carp summer high temperature death making according to technique scheme, can effectively improve black carp body's immunity, suppress simultaneously or kill various harmful bacteriums and virus in black carp body, strengthen black carp vigor, significantly reduce the generation of the common disease in summer such as black carp enteritis, or when the diseases such as enteritis occur, effectively alleviate disease symptoms, and reducing the death rate of black carp, its advantage is:
1) raw material is simple and easy to get, and cost is lower;
2) configuration is simple, easy to use;
3) can directly add in black carp feed oral throwing something and feeding, the use of also can splashing in water body;
4) nontoxic secondary residual, without the resistance to the action of a drug, environmental protection, to feed palatability without any harmful effect.
The specific embodiment
Below will to the present invention, do further statement in detail by specific embodiment and verification the verifying results test example, but the present invention is not limited in following embodiment.
Embodiment 1
Get Rhizoma Zingiberis extract 30%, n-propyl gallate 15%, tryptophan 25%, allicin 10%, vitamin E 10%, ethoxyquinoline 10%, mixes and is product of the present invention.
Immunopotentiator of the present invention is added in black carp expanded pellet diet according to the amount of 3.0kg/ ton, and black carp expanded pellet diet is produced by Zhejiang Hengxing Feedstuff Co., Ltd.The 7-12 month in 2012, in water chestnut lake, Huzhou City of Zhejiang Province, select 6 mouthfuls of black carp cultivating pools growth test of ingesting, the normal expanded pellet diet and add the feed of this immunopotentiator of throwing something and feeding respectively.Throw something and feed test feed after 2 months, in each pond, sample 10 tail black carps, measure its Liver Superoxide Dismutase Activity (SOD) activity, cultivation is cleaned up the pond and is sold fish to 6 mouthfuls of ponds after December, statistics black carp average specification, average yield per mu, the death rate and feed coefficient, result is as following table:
The impact of immunopotentiator on black carp immunocompetence, the death rate, body weight and per mu yield
| ? | Liver SOD (U/mg) | Average weight (kg) | Per mu yield (kg) | Death rate % | Feed coefficient |
| Control group | 47.25 | 4.9 | 999.6 | 32 | 2.7 |
| Immunopotentiator group | 75.27 | 5.4 | 1555.2 | 4 | 1.9 |
The determination data demonstration of upper table, adds this immunopotentiator in feed, black carp liver SOD activity has improved 59.3%, and average weight has improved 10.2%, and average yield per mu has improved 55.6%, and the death rate has reduced by 28%, and feed coefficient has reduced by 30%.
This result of the test explanation: use immunopotentiator of the present invention present situation to improve the immune level of black carp, promote the black carp speed of growth, reduce the death rate, improve bait conversion ratio, and successful.
Embodiment 2
Get Rhizoma Zingiberis extract 30kg, n-propyl gallate 15kg, tryptophan 25kg, allicin 6kg, vitamin E 20kg, ethoxyquinoline 4kg, mix.In water chestnut lake, Huzhou City of Zhejiang Province, break out flatly in July, 2012 in the pond that black carp enteritis disease causes black carp death continuously according to splash this immunopotentiator 7 days of 0.4ppm concentration, black carp enteritis disease symptom is effectively alleviated.In the follow-up cultivation in this pond, within every 15 days, use this immunopotentiator once, all according to 0.4ppm concentration, splash.Cultivation will be to the end of the year, and this pond totally cultivates survival rate 83%, not use this immunopotentiator pond to compare the death rate greatly reduce (the pond death rate of generally falling ill is at 30-50%) with periphery.The immunopotentiator of the present invention common bacterial diseases such as can effectively treating black carp enteritis in summer of splashing in water body is used in the explanation of this result of the test, reduces the black carp death causing because of this disease.
Embodiment 3
Get Rhizoma Zingiberis extract 25kg, n-propyl gallate 10kg, tryptophan 40kg, allicin 15kg, vitamin E 8kg, ethoxyquinoline 2kg mixes.Addition according to 0.1% adds this immunopotentiator in black carp feed, cultivates the black carp 60 days that initial body weight is about 12.3g in 3 300L aquariums.Separately there are 3 aquariums to throw something and feed not containing immunopotentiator feed as a control group.Each aquarium is put black carp 72 tails in a suitable place to breed.Cultivate after 60 days, select Aeromonas hydrophila to carry out challenge test as the source of infection, attack poison after 7 days, the control group death rate 35%, the immunopotentiator group death rate 17%.This result of the test shows that immunopotentiator of the present invention can effectively improve the ability of black carp opposing Aeromonas hydrophila.
The above, it is only preferred embodiment of the present invention, not the present invention is done to any pro forma restriction, therefore every, do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the modification above embodiment done according to technical spirit of the present invention, all still belong in the scope of technical solution of the present invention.
Claims (3)
1. prevent and treat the composite immune reinforcing agent of black carp summer high temperature death, it is characterized in that its raw material mainly comprises the each component of following mass percentage content: Rhizoma Zingiberis extract 20-40%, n-propyl gallate 5-15%, tryptophan 25-40%, allicin 6-17%, vitamin E 5-20%, ethoxyquinoline 2-10%.
2. prevent and treat according to claim 1 the composite immune reinforcing agent of black carp summer high temperature death, it is characterized in that the optimal case of composition of raw materials is: Rhizoma Zingiberis extract 30%, n-propyl gallate 15%, tryptophan 25%, allicin 10%, vitamin E 10%, ethoxyquinoline 10%.
3. according to the composite immune reinforcing agent of preventing and treating the death of black carp summer high temperature described in claim 1 or 2, it is characterized in that the preparation method of described Rhizoma Zingiberis extract is: get rhizoma zingiberis and dry, after the rhizoma zingiberis after drying is pulverized, be Rhizoma Zingiberis extract under 80-90 ℃ of condition.
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| CN103960495B (en) * | 2014-04-10 | 2015-12-30 | 合肥蓝华生物科技有限公司 | A kind of silkworm chrysalis high protein crab feed and preparation method thereof |
| CN105029045B (en) * | 2015-07-29 | 2018-11-06 | 广州美瑞泰科生物工程技术有限公司 | A kind of feeding additive aquatic animal |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294878A (en) * | 2000-11-17 | 2001-05-16 | 解俊峰 | Efficient composite nutrrent liquid |
| CN101053367A (en) * | 2006-04-14 | 2007-10-17 | 黄宏渊 | Preparation method of feed addictive |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294878A (en) * | 2000-11-17 | 2001-05-16 | 解俊峰 | Efficient composite nutrrent liquid |
| CN101053367A (en) * | 2006-04-14 | 2007-10-17 | 黄宏渊 | Preparation method of feed addictive |
Non-Patent Citations (2)
| Title |
|---|
| 许丹.长三角青鱼夏季高温死亡分析.《渔业致富指南》.2012,(第22期),第60页左栏末段，右栏倒数第2段. |
| 长三角青鱼夏季高温死亡分析;许丹;《渔业致富指南》;20121130(第22期);第60页左栏末段，右栏倒数第2段 * |
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