CN103355533A - Composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer and its use method - Google Patents
Composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer and its use method Download PDFInfo
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- CN103355533A CN103355533A CN2013103050629A CN201310305062A CN103355533A CN 103355533 A CN103355533 A CN 103355533A CN 2013103050629 A CN2013103050629 A CN 2013103050629A CN 201310305062 A CN201310305062 A CN 201310305062A CN 103355533 A CN103355533 A CN 103355533A
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Abstract
In order to solve the survival possibility of probiotics in high-temperature and high humidity environment and add them into aquatic microcapsule baits to produce probiotic-containing microcapsule baits suitable for shrimps, crabs, fry and the like, the invention discloses a composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer and its use method. The composite immunopotentiator mainly comprises the following raw materials by mass: 20-40% of a dried ginger extract, 5-15% of propylgallate, 25-40% of tryptophan, 6-17% of allicin, 5-20% of vitamin E, and 2-10% of ethoxy quinoline. The composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer can be blended into forage as a forage additive, and during feeding, its adding amount accounts for 0.1-0.5% of the mass of the forage; or the composite immunopotentiator can be splashed in a fish pond, and during splashing into the fish pond, the use concentration of the composite immunopotentiator is 0.2-0.4ppm. With the composite immunopotentiator provided in the invention, the Mylopharyngodon piceus's body immune function can be effectively improved, and the death rate of Mylopharyngodon piceus can be reduced.
Description
Technical field
The present invention relates to a kind of composite immune reinforcing agent, belong to technical field of aquaculture, particularly relate to a kind of composite immune reinforcing agent and the using method thereof that can prevent and treat the death of black carp summer high temperature.
Background technology
Black carp (Mylopharyngodon piceus) has another name called blue or green grass carp, blue, spiral shell green grass or young crops, black carp, black grass carp etc.Mainly be distributed in the plains region of drought, be one of CHINESE FRESHWATER cultivation " four large Chinese carps ", have a long cultivation in China historical.2011, Chinese black carp output added up 46.77 ten thousand tons, was the important economic freshwater fish in Yangtze River Delta Area.Nutritional need for black carp, existing a lot of experts have carried out system research, also developed the special-purpose black carp feed of part, such as Chinese patent application CN201110308066.3 (a kind of feed formula for black carp), Chinese patent application CN1692796 (a kind of health-care black carp feed) and Chinese patent application CN102771671A (a kind of efficient and environment-friendly type black carp expanding compound feed and production method thereof) etc.Yet along with the fast development of black carp intensive culture, we find in recent years, and the death rate obviously increases during the black carp fishing breeding pond summer high temperature of Yangtze River Delta Area.According to Relevant Analysis, this sick occurrence cause is that high-grade feed nutrition concentration is higher, and the additional deficiency such as vitamin, thereby cause black carp anti-stress ability under the too fast speed of growth to weaken relevant.At present, mostly be at antibiotic medicines such as the chemosterilant such as water splashing PVP-I or oral Enrofloxacins about the method for preventing and treating of this disease, there is the risk of medicament residue in these methods and to the secondary infringement of cultivation fish.Therefore, develop the immunity that a kind of composite immune reinforcing agent improves black carp self, reduce simultaneously effectively the death that black carp causes because of point-like aerogenesis monad during summer high temperature, the healthy growth tool of black carp is of great significance.
Summary of the invention
The object of the invention is to, a kind of immunopotentiator that can prevent and treat the death of black carp summer high temperature and preparation method thereof is provided, when improving the black carp general level of the health, can significantly reduce the generation of the diseases such as black carp enteritis in summer, or when enteritis disease occurs, effectively alleviate disease symptoms, reduce the death rate of black carp.
In order to achieve the above object, according to the present invention a kind of immunopotentiator of preventing and treating the death of black carp summer high temperature is proposed, it is characterized in that its raw material mainly comprises the each component of following mass percentage content: Rhizoma Zingiberis extract 20-40%, n-propyl gallate 5-15%, tryptophan 25-40%, allicin 6-17%, vitamin E 5-20%, ethoxyquinoline 2-10%.
The optimal case of composition of raw materials is: Rhizoma Zingiberis extract 30%, n-propyl gallate 15%, tryptophan 25%, allicin 10%, vitamin E 10%, ethoxyquinoline 10%.
The preparation method of Rhizoma Zingiberis extract of the present invention is: get rhizoma zingiberis and dry under 80-90 ℃ of condition, be Rhizoma Zingiberis extract after the rhizoma zingiberis after the oven dry is pulverized.
The using method of the immunopotentiator of control black carp summer high temperature death: be stirred in the feed as feed addictive, when throwing something and feeding, its addition is the 0.1-0.5% of feeding quality; Or splash in fishpond, when splashing in the fish pond, its working concentration is 0.2-0.4ppm.
In the raw material: rhizoma zingiberis warming spleen and stomach for dispelling cold, strong fortune spleen sun, in Chinese medicine, be used as and control deficiency-cold in spleen and stomach, coldness and pain in the epigastrium, vomiting is had loose bowels; N-propyl gallate is a kind of fat-soluble food antioxidant, usually as food additives, is used for preventing Food Oxidation and rotten.Tryptophan is a kind of important nutritional agents, can participate in the renewal of plasma proteins in the animal body, and can impel riboflavin to play a role, and also helps the synthetic of nicotinic acid and ferroheme, can significantly increase the antibody in the animal body, improves the immunity of animal body.Allicin has stronger antibacterial and anti-inflammation functions, multiple coccus, bacillus, fungi, virus etc. is all had suppress or killing action.Vitamin E is one of topmost antioxidant, and the injury that protection skin is avoided ultraviolet ray and polluted makes slightly blood vessel dilatation of end, improves blood circulation, can effectively improve body endurance, strengthen immunologic function.Ethoxyquinoline is one of feed antioxidant of function admirable, it is most economical antioxidant, be applicable to premix, fish meal and add the product of fat, can prevent that wherein vitamin A. D. E etc. and fatty oxygen from dividing rotten and natural colouring matter oxidation stain, and certain mildew-resistant and preservation are arranged.
The composite immune reinforcing agent of the control black carp summer high temperature death that makes according to technique scheme, energy Effective Raise black carp body's immunity, suppress simultaneously or kill various harmful bacteriums and virus in the black carp body, strengthen the black carp vigor, significantly reduce the generation of the common disease in summer such as black carp enteritis, or when the diseases such as enteritis occur, effectively alleviate disease symptoms, and reducing the death rate of black carp, its advantage is:
1) raw material is simple and easy to, and cost is lower;
2) configuration is simple, easy to use;
3) can directly add in the black carp feed oral throwing something and feeding, the use of also can in water body, splashing to;
4) nontoxic secondary residual, without the resistance to the action of a drug, environmental protection, to the feed palatability without any harmful effect.
The specific embodiment
Below will do further in detail statement to the present invention by specific embodiment and verification the verifying results test example, but the present invention is not limited in following embodiment.
Embodiment 1
Get Rhizoma Zingiberis extract 30%, n-propyl gallate 15%, tryptophan 25%, allicin 10%, vitamin E 10%, ethoxyquinoline 10% mixes and is product of the present invention.
Immunopotentiator of the present invention is added in the black carp expanded pellet diet according to the amount of 3.0kg/ ton, and the black carp expanded pellet diet is produced by Zhejiang Hengxing Feedstuff Co., Ltd.The 7-12 month in 2012, select 6 mouthfuls of black carp cultivating pools growth test of ingesting in Huzhou City of Zhejiang Province water chestnut lake, the normal expanded pellet diet and add the feed of this immunopotentiator of throwing something and feeding respectively.Throw something and feed test feed after 2 months, and sampling 10 tail black carps are measured its Liver Superoxide Dismutase Activity (SOD) activity in each pond, clean up the pond and sell fish in cultivation 6 mouthfuls of ponds after the December, the average specification of statistics black carp, average yield per mu, the death rate and feed coefficient, result such as following table:
Immunopotentiator is on the impact of black carp immunocompetence, the death rate, body weight and per mu yield
| ? | Liver SOD (U/mg) | Average weight (kg) | Per mu yield (kg) | Death rate % | Feed coefficient |
| Control group | 47.25 | 4.9 | 999.6 | 32 | 2.7 |
| The immunopotentiator group | 75.27 | 5.4 | 1555.2 | 4 | 1.9 |
The determination data of upper table shows, adds this immunopotentiator in the feed, and black carp liver SOD activity has improved 59.3%, and average weight has improved 10.2%, and average yield per mu has improved 55.6%, and the death rate has reduced by 28%, and feed coefficient has reduced by 30%.
This result of the test explanation: use immunopotentiator of the present invention present situation to improve the immune level of black carp, promote the black carp speed of growth, reduce the death rate, improve the bait conversion ratio, and successful.
Embodiment 2
Get Rhizoma Zingiberis extract 30kg, n-propyl gallate 15kg, tryptophan 25kg, allicin 6kg, vitamin E 20kg, ethoxyquinoline 4kg, mix.Break out flatly in Huzhou City of Zhejiang Province water chestnut lake in July, 2012 in the pond that the black carp enteritis disease causes black carp death continuously according to splash this immunopotentiator 7 days of 0.4ppm concentration, black carp enteritis disease symptom is effectively alleviated.In the follow-up cultivation in this pond, used this immunopotentiator once, and all splashed according to 0.4ppm concentration in per 15 days.Cultivation will be to the end of the year, and this pond totally cultivates survival rate 83%, not use this immunopotentiator pond to compare the death rate with periphery and greatly reduce (the pond death rate of generally falling ill is at 30-50%).The immunopotentiator of the present invention common bacterial diseases such as can effectively treating black carp enteritis in summer of splashing is used in the explanation of this result of the test in water body, reduce the black carp death that causes because of this disease.
Embodiment 3
Get Rhizoma Zingiberis extract 25kg, n-propyl gallate 10kg, tryptophan 40kg, allicin 15kg, vitamin E 8kg, ethoxyquinoline 2kg mixes.Addition according to 0.1% adds this immunopotentiator in black carp feed, the initial body weight of cultivation is about the black carp 60 days of 12.3g in 3 300L aquariums.Other has 3 aquariums to throw something and feed not contain the immunopotentiator feed and organizes in contrast.Each aquarium is put black carp 72 tails in a suitable place to breed.Cultivate after 60 days, select Aeromonas hydrophila to carry out challenge test as the source of infection, attack poison after 7 days, the control group death rate 35%, the immunopotentiator group death rate 17%.This result of the test show immunopotentiator of the present invention can the Effective Raise black carp ability of opposing Aeromonas hydrophila.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, therefore every technical solution of the present invention content that do not break away from,, all still belong in the scope of technical solution of the present invention any simple modification, equivalent variations and modification that above embodiment does according to technical spirit of the present invention.
Claims (5)
1. prevent and treat the composite immune reinforcing agent of black carp summer high temperature death, it is characterized in that its raw material mainly comprises the each component of following mass percentage content: Rhizoma Zingiberis extract 20-40%, n-propyl gallate 5-15%, tryptophan 25-40%, allicin 6-17%, vitamin E 5-20%, ethoxyquinoline 2-10%.
2. the composite immune reinforcing agent of described control black carp summer high temperature death according to claim 1 is characterized in that the optimal case of composition of raw materials is: Rhizoma Zingiberis extract 30%, n-propyl gallate 15%, tryptophan 25%, allicin 10%, vitamin E 10%, ethoxyquinoline 10%.
3. the composite immune reinforcing agent of described control black carp summer high temperature death according to claim 1 and 2 is characterized in that the preparation method of described Rhizoma Zingiberis extract is: get rhizoma zingiberis and dry under 80-90 ℃ of condition, be Rhizoma Zingiberis extract after the rhizoma zingiberis after will oven dry is pulverized.
4. the using method of the composite immune reinforcing agent of described control black carp summer high temperature death according to claim 1 is characterized in that being stirred in the feed as feed addictive, and when throwing something and feeding, its addition is the 0.1-0.5% of feeding quality.
5. the using method of the composite immune reinforcing agent of described control black carp summer high temperature death according to claim 1 is characterized in that splashing in fishpond, and when splashing in the fish pond, its working concentration is 0.2-0.4ppm.
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| CN201310305062.9A CN103355533B (en) | 2013-07-11 | 2013-07-11 | Composite immunopotentiator for control of Mylopharyngodon piceus death at high temperature in summer and its use method |
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| CN103355533B CN103355533B (en) | 2014-09-24 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960495A (en) * | 2014-04-10 | 2014-08-06 | 孙才海 | High-protein silkworm chrysalis feed for crabs and preparation method of high-protein silkworm chrysalis feed |
| CN105029045A (en) * | 2015-07-29 | 2015-11-11 | 广州美瑞泰科生物工程技术有限公司 | Additive for aquatic feed |
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| CN1294878A (en) * | 2000-11-17 | 2001-05-16 | 解俊峰 | Efficient composite nutrrent liquid |
| CN101053367A (en) * | 2006-04-14 | 2007-10-17 | 黄宏渊 | Preparation method of feed addictive |
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- 2013-07-11 CN CN201310305062.9A patent/CN103355533B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294878A (en) * | 2000-11-17 | 2001-05-16 | 解俊峰 | Efficient composite nutrrent liquid |
| CN101053367A (en) * | 2006-04-14 | 2007-10-17 | 黄宏渊 | Preparation method of feed addictive |
Non-Patent Citations (1)
| Title |
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| 许丹: "长三角青鱼夏季高温死亡分析", 《渔业致富指南》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960495A (en) * | 2014-04-10 | 2014-08-06 | 孙才海 | High-protein silkworm chrysalis feed for crabs and preparation method of high-protein silkworm chrysalis feed |
| CN103960495B (en) * | 2014-04-10 | 2015-12-30 | 合肥蓝华生物科技有限公司 | A kind of silkworm chrysalis high protein crab feed and preparation method thereof |
| CN105029045A (en) * | 2015-07-29 | 2015-11-11 | 广州美瑞泰科生物工程技术有限公司 | Additive for aquatic feed |
| CN105029045B (en) * | 2015-07-29 | 2018-11-06 | 广州美瑞泰科生物工程技术有限公司 | A kind of feeding additive aquatic animal |
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