CN1212292C - Microorganic fertilizer against various bacteria and its preparation and use - Google Patents
Microorganic fertilizer against various bacteria and its preparation and use Download PDFInfo
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Abstract
The present invention relates to a microbial fertilizer against various bacteria, which belongs to the field of a microbial fertilizer. The microbial fertilizer firstly provides a fertilizer and pesticide double-purpose concept, four kinds of good bacteria containing jelly sample spore bacillus, bacillus licheniformis, positive streptomycete, trichoderma, and the like with different efficiency are selected according to a bacterium-bacterium treating principle and are combined, and the microbial fertilizer against various bacteria having the functions of nutrition, antibiosis, growth promotion, etc. The present invention also provides a preparation method for the microbial fertilizer against various bacteria, which comprises the processing steps: bacterium suitable cultivation and fermentation condition determination, bacterium slant cultivation, erlenmeyer flask cultivation, fermentation tank fermentation and/or solid cultivation, bacterium agent compounding, carrier absorption, etc. The present invention also provides a method of the microbial fertilizer against various bacteria for the application of ground fertilizer, base fertilizer and top dressing fertilizer and in the modes of hole application, seed dressing, and the like and the application on cotton, wheat, hot peppers, tomatoes, eggplant, strawberries, asparagus lettuce, white gourds and tobacco.
Description
Technical field:
The present invention relates to microbial fertilizer and manufacture method thereof and application, specifically, the present invention relates to a kind of microbial fertilizer and manufacture method thereof with nutrition, biological control and growth-promoting functions, and this microbial fertilizer different administration mode and the application in the Different Crop cultivation.
Background technology:
Fertilizer is one of the most basic capital goods in the agricultural sector.What the mankind used the earliest is some Natural fertilizers, as muck, compost occurred through development afterwards, has occurred artificial manure then.Artificial manure the earliest is a chemosynthesis fertilizer, is called for short chemical fertilizer.Over more than hundred year, chemical fertilizer has been played the part of an important role in agriculture production.But along with the increase of applying quantity of chemical fertilizer, chemical fertilizer is to the destruction of Soil structure, to the negative impact of ecotope, and also more and more serious to the infringement of agricultural product security.
What almost come out simultaneously with chemical fertilizer is the microbial fertilizer of representative with root nodule bacterium, and its original intention will provide crop growth needed nutrition from ecological angle exactly, to overcome a series of problems that chemical fertilizer brings.Owing to it is believed that at that time that microbial fertilizer can not be directly for plant provides nutrient, thereby make this technical development slow.Up to the second half in last century, after the sustainable development of problem of environmental pollution and agriculture production was mentioned agenda, s-generation microbial fertilizer just really had been born.
S-generation microbial fertilizer is based on nutritional type.By the activity of microorganism, decompose the phosphorus or the potassium of indissoluble in the soil, the airborne nitrogen of assimilation provides nutrient for plant.S-generation microbial fertilizer is because its biological safety has obtained significant progress over nearly 30 years.The microbial fertilizer that present China sells on the market belongs to s-generation microbial fertilizer more.
S-generation microbial fertilizer has important effect to improving the ecological environment, improve biological safety, but provide overlapping on the function of plant nutrient merely with the function of chemical fertilizer with regard to it, and the quick-acting of microbial fertilizer is good not as chemical fertilizer, and these shortcoming have influenced further developing of s-generation microbial fertilizer.
In addition, continuous cropping is in agriculture production and general, and the soil-borne disease that causes thus is very serious, and soil-borne disease is particularly outstanding in facility cultivation, and these problems can't be overcome by chemical fertilizer or using of s-generation microbial fertilizer.
Summary of the invention:
The objective of the invention is in order to remedy the above-mentioned deficiency of chemical fertilizer and present microbial fertilizer, break through continuous cropping obstacle, overcome the soil-borne disease problem, take the lead in proposing the notion of fertile medicine economic benefits and social benefits, select the combination of the excellent species of different efficacies according to the principle of controlling bacterium with bacterium, develop have nutrition, antibiosis and short microorganism multi-antibacterial manure of functions such as giving birth to.
The present invention has selected four bacterial classification combinations for use.The bacterial strain that can promote plant growth that wherein has also has the secretion antibiotic substance to suppress the bacterial strain of pathogenic bacteria growth in the soil.Different with common chemical fertilizer and chemical pesticide, biological multi-antibacterial manure is not directly to provide nutrition to crop, but the vital movement of utilizing the live microorganism that contains in the biological multi-antibacterial manure is improved the crop rhizosphere ecotope, it is available quick-acting nutritious for crop to transform the nutritive element that crop not directly utilizes in the soil, and bacterial strain can also be secreted short biomass stimulates plant growth.Aspect curing the disease, biological multi-antibacterial manure adopts the principle of controlling bacterium with bacterium, utilizes antagonistic strain excretory antibiotics to suppress growth of pathogenic bacteria in the soil, makes pathogenic bacteria can't reach pathogenic quantity in soil, thereby can't infect crop, makes the crop morbidity.Through extensive field test, the result shows that this microorganism multi-antibacterial manure is on wheat, paddy rice, corn, cotton, vegetables and melon crop, not only can improve output, improve quality, can also prevent and treat the soil-borne disease of farm crop well, the effect aspect industrialized agriculture is more remarkable.
Another object of the present invention provides a kind of manufacture method for preparing microorganism multi-antibacterial manure.
The present invention has also furtherd investigate the multiple method of application and the result of use in the Different Crop cultivation of mentioned microorganism multi-antibacterial manure.
Technical scheme of the present invention is as follows:
1) bacterial classification selects for use
The present invention has selected for use four bacterial classifications as follows respectively:
A, bacillusmusilaginosiengineering (Bacillus mucilaginous Krassilnikov) China Committee for Culture Collection of Microorganisms agricultural microorganism center, bacterium numbering: ACCC10091;
B, Bacillus licheniformis (Bacillus licheniformis (Weigmann) Chester) China Committee for Culture Collection of Microorganisms agricultural microorganism center bacterium numbering: ACCC10613;
C, Jingyang streptomycete (Streptomyces jingyangensis Tao et al.); China Committee for Culture Collection of Microorganisms agricultural microorganism center, bacterium numbering: ACCC30409;
D, wood mould (Trichoderma sp.) China Committee for Culture Collection of Microorganisms agricultural microorganism center, bacterium numbering: ACCC40127.
Above-mentioned 4 bacterial classification public can buy to China Committee for Culture Collection of Microorganisms agricultural microorganism center by bacterium numbering.
2) strain fermentation
Biological nature separately according to above-mentioned 4 different strains is cultivated, is fermented.
3) microbial inoculum is composite
4 different microbial inoculums are mixed by a certain percentage.Preferred version is: the fermented liquid that bacillusmusilaginosiengineering is made through strain fermentation, the fermented liquid that Bacillus licheniformis is made through strain fermentation, solid culture, the mould solid culture of making of wood that the Jingyang streptomycete is made, four weight ratio is: 3.0-3.5: 3.0-3.5: 1.5-2.0: 1.5-2.0.
4) carrier of bacterium liquid absorption
With bacterium liquid and carrier thorough mixing.
The present invention has " fertilizer-medicine " double effects by nutritional type microorganism and the short composite microorganism multi-antibacterial manure that forms of type microorganism of giving birth to of biological and ecological methods to prevent plant disease, pests, and erosion, can significantly promote the generation of plant growth and reduction soil-borne disease.Owing to select composite the forming of multiple antagonism bacterium for use, have antimicrobial spectrum more widely, multiple pathogenic fungi and pathogenetic bacteria are had stronger inhibitory or killing effect.
Embodiment:
The fermentation of embodiment 1 bacillusmusilaginosiengineering
1) substratum: pH:7.0-7.2
Inclined-plane and shaking table are used: sucrose: 10g; K
2HPO
4: 0.5g; MgSO
47H
2O:0.2g; Yeast extract paste: 0.4g; CaCO
3: 1g; FeCl
3: 0.005g; Add agar: 15-20g during solid culture in addition.
Fermentor tank is used: sucrose 0.1%, Semen Maydis powder 5%, soybean cake powder 1%, K
2HPO
4: 0.05%, MgSO
47H
2O:0.02%, yeast extract paste 0.04%, CaCO
3: 0.1%, FeCl
3: 0.0005%
2) cultivation and fermentation process
Slant culture is used: cultivated 36-48 hour for 28 ℃ ± 2 ℃.
Triangular flask liquid shaking table is cultivated: 28 ℃ ± 2 ℃, cultivated 200 rev/mins 36-48 hour;
The fermentation of one grade fermemtation jar: 28 ℃ ± 2 ℃, to cultivate 36-48 hour, air flow is (volume ratio of per minute air flow and fermented liquid) between 0.1-1.
The fermentation of second order fermentation jar: 28 ℃ ± 2 ℃, to cultivate 36-48 hour, air flow is (volume ratio of per minute air flow and fermented liquid) between 0.1-1.
The three grade fermemtation jar: 28 ℃ ± 2 ℃, to cultivate about 48 hours, air flow is (volume ratio of per minute air flow and fermented liquid) between 0.1-1.
Gained bacterium liquid is to call bacterium liquid 1 in the following text.
Embodiment 2 the lichen bacillus ferments
1) substratum: g/1000ml distilled water pH:7.0
Inclined-plane and shake bottle and use culture medium protein peptone: 5g; NaCl:5g; Extractum carnis: 3g; Add agar: 15-20g (solid culture adds) during solid culture in addition
Fermentor tank substratum soybean cake powder 2%, Semen Maydis powder 10%, K
2HPO
4: 0.05%, MgSO
47H
2O:0.02%, yeast extract paste 0.04%, NaCl:0.5%,
2) cultivation and fermentation process
Slant culture: 28 ℃ ± 2 ℃, cultivated 36-48 hour.
Triangular flask liquid shaking table is cultivated: 28 ℃ ± 2 ℃, cultivated 200 rev/mins 36-48 hour.
The fermentation of one grade fermemtation jar: 28 ℃ ± 2 ℃, to cultivate 36-48 hour, air flow is (volume ratio of per minute air flow and fermented liquid) between 0.1-1.
The fermentation of second order fermentation jar: 28 ℃ ± 2 ℃, to cultivate 36-48 hour, air flow is (volume ratio of per minute air flow and fermented liquid) between 0.1-1.
The three grade fermemtation jar: 8 ℃ ± 2 ℃, to cultivate about 48 hours, air flow is (volume ratio of per minute air flow and fermented liquid) between 0.1-1
Gained bacterium liquid is to call bacterium liquid 2 in the following text.
Embodiment 3 Jingyang streptomycete fermentations
1) substratum: g/1000ml distilled water pH:7.2-7.4
Inclined-plane and preservation KNO
3: 1g; NaCl:0.5g; MgSO
47H
2O:0.5g; K
2HPO
4: 0.5g; FeSO
47H
2O:0.01g; Zulkovsky starch: 20g; Agar: 20g.
Solid fermentation wheat bran 80%, straw powder 19%, KH
2PO
4: 0.5%, (NH
4)
2SO
4: 0.5%, CaCl
20.2%
Material: water=1: 2.2
2) cultivation and fermentation process
Slant culture: 22 ℃ ± 2 ℃.48 hours
Triangular flask solid culture: 22 ℃ ± 2 ℃.48 hours
Solid fermentation is cultivated: 22 ℃ ± 2 ℃.48 hours
The gained culture is to call microbial inoculum 3 in the following text.
Embodiment 4 wooden mould fermentations
1) substratum: g/1000ml distilled water
Inclined-plane and preservation are used: peeling potato 200g cuts fritter, adds water 1000ml and boils 30 minutes, and the elimination potato ball is supplied 1000ml with filtrate, adds glucose 20g, agar 15g.
Solid fermentation: wheat bran 30%, straw powder 70%, KH
2PO
4: 0.5%, (NH
4)
2SO
4: 0.5%, CaCl
20.2% material: water=1: 2.2
2) cultivation and fermentation process
Slant culture: 22 ℃ ± 2 ℃.48 hours
Triangular flask solid culture: 22 ℃ ± 2 ℃.48 hours
Solid fermentation is cultivated: 22 ℃ ± 2 ℃.48 hours
The gained culture is to call microbial inoculum 4 in the following text.
The fermentor tank of using among the embodiment 1-4 is mechanical agitating fermentation tank
Embodiment 5 microbial inoculums are composite
With bacterium liquid 1, bacterium liquid 2, microbial inoculum 3, microbial inoculum 4 is combined, and weight ratio is 3.5: 3.5: 1.5: 1.5.
The carrier absorption of embodiment 6 bacterium liquid
With embodiment 5 gained bacterium liquid and peat composed of rotten mosses thorough mixing, absorption back total water content is between 25%-30%, and weight ratio is: 1: 3, packing went out finished product.
Embodiment 7 application of biological multi-antibacterial manure on cotton
The blight fungal pathogens is the two kinds of fast-spreading soil biography property of mat soil, seed and plant residue fungies, just can cause the cotton significantly underproduction and the reduction of gined cotton quality in case infect popular back, is that Cotton in China is produced two kinds of the most serious diseases of harm.China Agriculture Academe Fertilizer Institute has carried out the potted plant and field plot trial of biological multi-antibacterial manure aspect the cotton disease-preventing and yield-increasing.
Potted plant: cotton seed adopts " anti-No. three of Hubei Province ", soil is yellowish-brown loam, fertilizer is biological multi-antibacterial manure, the former bacterium of the fusarium wilt of cotton is a sharp sickle spore wilting specialized form fungi (Fusarium oxysporum f.sp.vasinfecfum is hereinafter to be referred as F.o), and the former bacterium of cotton verticillium wilt is Garden Dahlia Verticillium (Verficillium dahliac is hereinafter to be referred as V.d).6 processing repeat 5 times, do not inoculate contrast (CK1) that is:, and every basin adds the bacterial manure seed dressing of 2 gram sterilising treatment; The F.o seed dressing is withered disease contrast (CK2); The V.d seed dressing is verticillium evil contrast (CK3); The seed dressing of F.o+ bacterial manure; V.d+ bacterial manure seed dressing: F.o+V.d+ bacterial manure seed dressing.Sow 4 of every basin final singlings behind 14 days meter seedling rates, sampling every basin final singling 2 strains in back in 35 days, the every basin in sampling back stayed 1 strain in 45 days.
The sub-district: (" Hubei Province cotton 18 ") carried out field plot trial in Laohekou cotton region, Hubei Province, and soil type is a yellowish soil.Two processing are established in test: (1) CK is not for executing biological multi-antibacterial manure, and (2) are treated to biological multi-antibacterial manure and dress seed by 500 gram/mus, simultaneously, make nutrition pot in fertilizer/soil than 1/1000 ratio mixing, and transplant the back of growing seedlings.Adopt three repetitions, randomized block design, sub-district area 40m
2Test each sub-district and execute 100 kilograms of fertilisings of fertilizer special for cotton by every mu, treatment zone is used with base fertilizer by every mu of 4 kilograms of biological multi-antibacterial manures, imposes bell fertilizer (urea) 0.25Kg/40m2, other field management unanimity.The result is as table 1-3.
The biological multi-antibacterial manure of table 1 is to cotton growth and morbific influence
The biological multi-antibacterial manure of table 2 becomes the influence of seedling quality to cotton
The biological multi-antibacterial manure of table 3 is to the influence of output of cotton and disease
| Handle | Year | Individual plant bell (individual) | Dan Lingchong (gram) | Ginning outturn (%) | Sub-district lint yield (Kg/ mu) | Volume increase (%) | Withered diseased plant (%) | Verticillium strain (%) | |||
| 1 | 2 | 3 | On average | ||||||||
| Contrast | 1999 | 8.2 | 5.78 | 41.2 | 88.03 | 85.86 | 87.23 | 87.04 | 32 | 30 | |
| 2000 | 8.1 | 5.70 | 41.1 | 81.25 | 82.61 | 85.20 | 83.20 | 35 | 30 | ||
| Handle | 1999 | 9.3 | 5.89 | 42.3 | 101.94 | 104.71 | 97.58 | 101.23 | 16.30 | 8 | 12 |
| 2000 | 9.1 | 5.85 | 42.1 | 93.60 | 93.30 | 88.35 | 91.75 | 10.52 | 7 | 11 | |
The above results shows that biological multi-antibacterial manure can not only prevent and treat disease, and can also promote cottonseed to germinate, promote growth, no matter be in blight or the single region of disease of verticillium, still mixing a district at blight all is a kind of good biological control method, and using in no lesion also has the effect that increases output and income.
Embodiment 8 application of biological multi-antibacterial manure on tomato
Tomato belongs to solanaceous vegetable, is one of govern-house-variety of various places vegetable growing.The generation intensity of nutrient supply ability and disease and pest all can influence the growth of tomato.
Experimental control is managed in the usual way, does not use multi-antibacterial manure; Be treated under the conventional fertilizer application situation, enrich multi-antibacterial manure by 3 kilograms of/mu standards.Test-results is as table 4-5.
Table 4: the high statistical unit of open country tomato strain: CM
| Fertilizer type | Plant height | On average | Effect relatively |
| Multi-antibacterial manure | 47.52 48.53 47.45 35.41 47.53 53.47 47.47 45.45 51.47 38.48 | 46.28 | Use multi-antibacterial manure, the average plant height of tomato improves 22.27% than the average plant height of composite fertilizer's contrast, and the short fruit of coming into force is obvious. |
| Composite fertilizer | 40.39 47.38 37.39 32.39 33.32 35.23 40.33 36.37 | 37.85 |
Table 5: biological multi-antibacterial manure is to tomato growth and morbific influence
| The sub-district | Kind | Area (mu) | Folding per mu yield (kilogram) | Volume increase (%) | Withered strain rate (%) | Reduce withered strain rate % |
| Handle | Tomato | 0.4 | 3132 | 13.87 | 4 | 66.7 |
| Contrast | Tomato | 3 | 2470 | 12 |
Table 6: biological multi-antibacterial manure is to tomato growth and morbific influence
| The sub-district | Kind | Area (mu) | Folding per mu yield (kilogram) | Volume increase (%) | Withered strain rate (%) | Reduce withered strain rate % |
| Handle | Tomato | 0.7 | 3117.6 | 13.87 | 4.1 | 74.6 |
| Contrast | Tomato | 2.5 | 2737.8 | 45 |
Test shows, uses biological multi-antibacterial manure and can effectively promote tomato growth, and is disease-resistant, effect of increasing production is apparent in view.
Embodiment 9 application of biological multi-antibacterial manure on capsicum
1) disease resisting effect
Soil-borne disease such as capsicum epidemic disease, blight happens occasionally, and has a strong impact on the income of pepper planting peasant household; Irrational agricultural chemicals uses, and has also increased edible and environmental safety hidden danger greatly.Table 7 shows that biological multi-antibacterial manure has significantly reduced the generation of pepper diseases.
The application of the biological multi-antibacterial manure of table 7 on pepper disease resistance
| The sub-district | Area (mu) | Method | Blight incidence (%) | Sickness rate reduces (%) |
| Handle | 0.5 | Use bacterial manure by 3 kilograms/mu amount during transplanting | 5 | 89.6 |
| Contrast | 0.5 | Multi-antibacterial manure is not used in management in the usual way | 48 | -- |
2) the short fruit of coming into force
The biology multi-antibacterial manure promotes the test of growth, is the result after contrasting by the composite fertilizer's processing with local routine.Test shows (table 8～10), and at the different times of chili growth, multi-antibacterial manure is handled all tangible promotion growth result.
Table 8 individual plant branch statistical analysis table
| Handle | Number of observation | Multi-antibacterial manure | Composite fertilizer | Increase, subtract (+,-) than composite fertilizer | Multi-antibacterial manure | Composite fertilizer | Compare composite fertilizer | |||||||
| Add up to | On average | Add up to | On average | Radix (individual) | Observation number (individual) | Have a net increase of number (individual) | Radix (individual) | Observation number (individual) | Have a net increase of number (individual) | Increase, subtract (+,-) | Have a net increase of rate (%) | |||
| 1 | 16 | 499.3 | 31.2 | 504.1 | 31.5 | -0.3 | 7.3 | 31.2 | 23.9 | 8.3 | 31.5 | 23.2 | 0.7 | 3 |
| 2 | 16 | 510.3 | 31.9 | 465.7 | 29.1 | 2.8 | 9.7 | 31.9 | 22.2 | 8 | 29.1 | 21.1 | 1.1 | 5.2 |
| 3 | 16 | 521.7 | 32.6 | 446.3 | 27.9 | 4.7 | 8.3 | 32.6 | 24.3 | 8.3 | 27.9 | 19.6 | 4.7 | 24 |
| On average | 32.6 | 29.5 | 3.1 | 24.6 | 21.3 | 3.3 | 16.1 | |||||||
Table 9 individual plant plant height statistical analysis table
Table 10 individual plant several cartograms of bearing fruit
Table 11 sub-district early yield comparative analysis table
| Multi-antibacterial manure | Composite fertilizer | Multi-antibacterial manure than composite fertilizer per mu yield relatively | Remarks | |||||||||||||||||
| January 5 | 9 | 15 | 20 | 25 | 30 | The output kilogram | Output value unit | January 5 | 9 | 15 | 20 | 25 | 30 | The output kilogram | Output value unit | The volume increase kilogram | Stimulation ratio % | Volume increase value unit | ||
| 1 | 0.77 | 0.8 | 1.7 | 3.4 | 4.3 | 3.8 | 14.7 | 16.2 | 0.84 | 1.5 | 1.1 | 2.4 | 1.8 | 5.4 | 12.9 | 14.2 | 121 | 13.9 | 133 | 1.1 yuan of capsicum per kilograms |
| 2 | 0.62 | 0.8 | 1.5 | 4.8 | 5.7 | 5.5 | 18.8 | 20.7 | 0.77 | 1 | 1.9 | 2.7 | 1.9 | 3.1 | 11.4 | 12.5 | 500 | 68.1 | 550 | |
| 3 | 0.44 | 0.8 | 2.2 | 4.3 | 5.2 | 6.2 | 19 | 20.9 | 0.61 | 0.9 | 1.8 | 3.5 | 3.8 | 4.9 | 15.5 | 17 | 240 | 23.2 | 264 | |
Table 12 single plant yield and number cartogram
Table 13 sub-district yield of hot pepper statistical analysis table
Illustrate: 1, the capsicum per kilogram is by 0.8 yuan of calculating.2, fruit is received 10 times altogether in every sub-district.
3) effect of increasing production
Test shows (table 7～13), by the performance of multi-antibacterial manure biological and ecological methods to prevent plant disease, pests, and erosion and growth-promoting functions, can be that the upgrowth situation of capsicum is even more ideal, thereby make output be able to obvious raising.We in addition think, be necessary further to test, inquire into to use biological multi-antibacterial manure to reduce the possibility of composite fertilizer's consumption, promote the coordinated development of economy and environmental benefit better.
Embodiment 10 application of biological multi-antibacterial manure seed dressing on wheat
Basic condition experimental field: soil property is a loam, and the previous crops wax gourd is executed 75 kilograms in bicarbonate of ammonia for every mu when October 15 was put in order ground, and calcium superphosphate is made base fertilizer for 35 kilograms, ploughs under in the soil with plough.Experiment and demonstration field area is 1.5 mu,
Method of application: compound bio multi-antibacterial manure seed dressing, 1 kilogram of every mu of consumption, using method is with a small amount of clear water wheat seeds to be mixed earlier wetly, pours the compound bio multi-antibacterial manure again into, makes every wheat seeds skin all be stained with bacterial manure through repeatedly rubbing, the back of waiting to dry in the shade is sowed.Do not dress seed for other 0.5 mu, directly sowing compares.The kind that wheat is selected for use is: super wheat EX-001-9, belong to large spike, and the mu application rate is 6.5 kilograms.
October 20 emerged, and chemical weeding on November 18 is once throughout the growing season manually hoed up weeds and loosened the soil.This experimental field is the vegetable plot, and because of not planting wheat for many years, the householder is afraid of the wheat lodging, does not all topdress, but observes during whole wheat growth, fertile phenomenon do not occur taking off.
The fringe that all sprouted April 5, because how overcast and rainy wheat grain filling stage in this year weather is, illumination reduces relatively, and thousand grain weight of wheat has been caused certain influence, in this case, we sample respectively test-results are carried out comprehensive species test, result such as table 14 before the harvesting.
Table 14 wheat uses biological multi-antibacterial manure seed dressing effect
Found that, observe from the whole wheat growth phase that use the wheat seedling leaf color unanimity of biological multi-antibacterial manure seed dressing dark green, tiller number increases, dial after the joint heading plant comparison according to increase, healthy and strong, root system is many and flourishing, cane is more resistant to lodging.By species test, spike length, spikelet number, grain number per spike, effective tillering, thousand seed weight comparison increase according to all having in various degree.With 349 kilograms of the per mu yields of biological multi-antibacterial manure seed dressing, than 307 kilograms of the per mu yields of not seed dressing.
Embodiment 11 biological multi-antibacterial manures are as the application of base manure on wheat
Basic condition experimental field: soil property is a loam, and preceding stubble is a corn, after a heatable brick bed soil of turning over, when ploughing, October 18 executes 50 kilograms in bicarbonate of ammonia for every mu, 25 kilograms of calcium superphosphate, 2 kilograms of (recommendeds rate of fertilizer application that contrast ground is same of biological multi-antibacterial manure, but do not add antibiotic fertilizer), plough under in the soil with plough.The wheat cultivation amount is 10 kilograms, and wheat breed is No. 1, Yibin.
Because good conditions for soil moisture during autumn sowing last year, October 20 all emerged, and chemical weeding on November 26 is once throughout the growing season manually hoed up weeds and loosened the soil, and every mu chases after 5 kilograms in urea, fertile phenomenon do not occur taking off from throughout the growing season observing wheat.
The fringe that all sprouted April 8, because how overcast and rainy wheat grain filling stage in this year weather is, illumination reduces relatively, and thousand grain weight of wheat has been caused certain influence, in this case, we sample respectively test-results are carried out comprehensive species test, result such as table 15 before the harvesting.
The biological multi-antibacterial manure of table 15 as base manure on wheat
Found that, observe from the whole wheat growth phase that it is dark green to use biological multi-antibacterial manure to make the wheat seedling leaf color unanimity of base manure, tiller number increases, dial after the joint heading plant comparison according to increase, healthy and strong, root system is many and flourishing, cane is more resistant to lodging.By species test spike length, spikelet number, grain number per spike, effective tillering, the contrast of dry granular anharmonic ratio increase is arranged all.Per mu yield increases by 40 kilograms, volume increase 13.2%, obvious effect of increasing production.
The application on asparagus lettuce of spreading manuer in holes, topdress of embodiment 12 biological multi-antibacterial manures
Basic condition experimental field: area is 1.2 mu, and soil is silty loam, preceding stubble cowpea, stubble before gathering in the crops August 15, September 12 ploughed, when ploughing, execute 50 kilograms of ternary compound fertilizers for every mu, 50 kilograms in bicarbonate of ammonia, the bedding of whole ground subsequently, wide 1.8 chis of furrow, 6 cun of furrow widths were transplanted asparagus lettuce on September 15, line-spacing 1.2 chis, spacing in the rows 1 chi, every mu of density 5000 strains, the asparagus lettuce kind is two white skins, and August 10 grew seedlings, 5 true leaves during transplanting.
Test design: test design is to plant the back at asparagus lettuce to establish two processing at random in the field: 1) execute biological multi-antibacterial manure; 2) not executing biological multi-antibacterial manure compares.Application process is topdressed at asparagus lettuce rhizosphere trench digging for spreading manuer in holes, and dressing time is afternoon September 23,2.5 kilograms of mu consumptions, and field management is hoed up weeds and is loosened the soil twice, and drought resisting is once.Gathered in the crops November 10, before receiving different treatment has been seen Table 16 in the concrete data of field sample survey.
The biological multi-antibacterial manure of table 16 spread manuer in holes, topdress application length (cm), weight (kg) on asparagus lettuce
Using biological multi-antibacterial manure as can be seen from Table 16 can increase production significantly, leaf is long, leaf is wide, stem garden, stem long and single-strain fresh weight all apparently higher than contrast.
Embodiment 13 biological multi-antibacterial manures are as the application of base fertilizer on broad bean
Broad bean kind: open beans No. one; Experimental field, each one mu on contrast ground, experimental field execute 2 kilograms of biological multi-antibacterial manures, other field management unanimity.Result such as table 17.
The biological multi-antibacterial manure of table 17 is as the application of base fertilizer on broad bean
Show that by test biological multi-antibacterial manure strengthens branch principal stresses, big, the 100-grain weight increase of pod, high yield character is good, and disease resistance is strong, and wide adaptability has the low-grade infection disease, and mu increases production 33 kilograms, volume increase 19%; Multiple diseases is infected in contrast.
Embodiment 14 application of biological multi-antibacterial manure on wax gourd
The wax gourd blight is the main soil-borne disease that the restriction wax gourd produces, in actual production harm bigger, still do not have ideal safety prevention and treatment method at present.
Experimental control is managed in the usual way, does not use multi-antibacterial manure; Be treated to when transplanting and execute multi-antibacterial manure by 3 kilograms/mu.Test-results such as following table 18.
The application of the biological multi-antibacterial manure of table 18 on wax gourd
| The sub-district | Area (mu) | Blight incidence % | Sickness rate reduces % |
| Handle | 1 | 4 | 91 |
| Contrast | 4 | 42 |
Test shows that biological multi-antibacterial manure has significantly prevention effect to the wax gourd blight, can effectively reduce the sickness rate of wax gourd blight.
Embodiment 15 application of biological multi-antibacterial manure on strawberry
Strawberry is owing to protection ground multiple crop index height, rotation of crops difficulty, and environment is airtight, humidity is bigger, thereby causes the continuous cropping obstacle of strawberry production very serious, and disease incidence of soil-borne disease such as verticillium, blight rise year after year, have a strong impact on the performance of strawberry cultivating benefit.The test booth is 4 of warm-in-winter plastic greenhouses (0.5 mu/canopy), and kind is Japanese Feng Xiang, every canopy field planting 3500 strains.The contrast booth is used as base manure for 1500 kilograms with 50 kilograms of compound potassium sulfate fertilizers, farm manure, handles booth and uses as base manure for 1.5 kilograms with 50 kilograms of compound potassium sulfate fertilizers, 1500 kilograms of farm manures and biological multi-antibacterial manure.The incidence of multiple strawberry disease, result such as table 19 have been investigated in test.
The application of the biological multi-antibacterial manure of table 19 on strawberry
| Verticillium sickness rate (%) | Blight sickness rate (%) | Bacterial wilt sickness rate (%) | Anthrax sickness rate (%) | Gray mold sickness rate (%) | |
| Contrast | 33.6 | 33.4 | 22.8 | 54.0 | 42.5 |
| Multi-antibacterial manure is handled | 4.9 | 2.9 | 3.4 | 2.1 | 3.8 |
| Reduce sickness rate | 85.4 | 91.3 | 85.1 | 96.1 | 91.1 |
Test shows that biological multi-antibacterial manure can be prevented and treated multiple soil-borne disease, can effectively improve the economic benefit of protection ground strawberry cultivating.
Embodiment 16 application of biological multi-antibacterial manure on eggplant
The factor that influences the eggplant growth is a lot, and comparatively outstanding is multiple soil-borne disease.Experimental control is managed in the usual way, does not use multi-antibacterial manure; Be treated under the conventional fertilizer application situation, enrich multi-antibacterial manure by 3 kilograms of/mu standards.Test-results such as table 20.
The application of the biological multi-antibacterial manure of table 20 on eggplant
| Treatment zone mean number | Check plot mean number | |||||
| Date | Plant height (cm) | Diseased plant rate % | Output (kilogram) | Plant height (cm) | Diseased plant rate % | Output (kilogram) |
| July 27 | 122.3 | 1.0 | 116.0 | 121.0 | 3.5 | 110.5 |
| August 3 | 126.0 | 1.5 | 131.0 | 124.2 | 5.5 | 126.8 |
| August 10 | 126.8 | 2.0 | 127.8 | 125.1 | 7.0 | 120.0 |
| August 18 | 127.2 | 3.0 | 109.5 | 126.0 | 7.5 | 107.7 |
| Increase anti-effect | Increase by 1.2 | Few 60% | Increase by 19.3 | The check plot | ||
Test shows that it is apparent in view to use this fertilizer district disease-resistant yield-increasing effect, and plant height increases 1.2cm, and diseased plant rate reduces 60%, 193 kilograms of every mu of volume increase of units increased in production.
Embodiment 17 biological multi-antibacterial manures are as the application of base fertilizer on tobacco
Amount of application: 2 kilograms every mu.Contrast need not biological multi-antibacterial manure, and other fertilizer amount is all consistent with field management.Basic condition is as follows: the tobacco bred of plantation: NC89, seed disinfection before the sowing.Ground is ground in winter slack.Seedling raise period: February 5, transplanting time: April 15.Rate of fertilizer application: 40 kilograms of sealing fertilizers.Economic target such as table 20.
The biological multi-antibacterial manure of table 21 is as the application of base fertilizer on tobacco
Find by field observation and economic target analysis, biological multi-antibacterial manure is made base fertilizer and can obviously be improved tobacco production and quality, the qualification rate of grade all within the requirement of national regulation, is observed at growing period, and multiple diseases such as black shank, anthrax, Powdery Mildew are all had restraining effect.
Claims (6)
1. microbial-bacterial fertilizer with fertile medicine economic benefits and social benefits is characterized in that this microbial-bacterial fertilizer contains following 4 kinds of bacterium liquid or microbial inoculum:
The fermented liquid that A, bacillusmusilaginosiengineering (Bacillus mucilaginous Krassilnikov) are made through strain fermentation, weight is 3.0～3.5 parts;
The fermented liquid that B, Bacillus licheniformis (Bacillus licheniformis (Weigmann) Chester) are made through strain fermentation, weight is 3.0～3.5 parts;
The solid culture that C, Jingyang streptomycete (Streptomyces jingyangensis Tao et al.) are made, weight is 1.5～2.0 parts;
The solid culture that D, wood mould (Trichoderma sp.) are made, weight is 1.5～2.0 parts.
2. prepare the method for microbial-bacterial fertilizer as claimed in claim 1, step comprises:
(1) selecting for use bacillusmusilaginosiengineering, Bacillus licheniformis, Jingyang streptomycete and wood mould is microorganism anti-calamities fertilizer;
(2) bacillusmusilaginosiengineering is cultivated through slant culture, triangular flask with Bacillus licheniformis and is become fermented liquid with ferment tank; The Jingyang streptomycete is cultivated into solid culture through slant culture, triangular flask solid culture with solid fermentation with wood is mould;
(3) above-mentioned fermented liquid and solid culture microbial inoculum is composite;
(4) composite bacteria liquid with the composite gained of (3) microbial inoculum carries out carrier absorption.
3. preparation method as claimed in claim 2 is characterized in that middle bacillusmusilaginosiengineering of described step (2) and Bacillus licheniformis are through 3 grades of ferment tanks.
4. preparation method as claimed in claim 2 is characterized in that the fermentor tank in the described step (2) is a mechanical agitating fermentation tank.
5. the application of the described microbial-bacterial fertilizer of claim 1 in arable farming.
6. the application of microbial-bacterial fertilizer as claimed in claim 5 is characterized in that this microbial-bacterial fertilizer is as base fertilizer, base manure and topdress and spread manuer in holes and dress seed; Described crop is cotton, tomato, capsicum, wheat, asparagus lettuce, broad bean, wax gourd, strawberry, eggplant or tobacco.
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Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101168491B (en) * | 2006-10-24 | 2012-07-04 | 北京新纪元三色生态科技有限公司 | Microorganism soil repairing agent and its preparation method and application |
| CN101747095B (en) * | 2008-12-05 | 2012-09-26 | 吉林农业大学 | Ginseng and American ginseng microorganism multi-antibacterial manure and preparation method thereof |
| CN102080047B (en) * | 2010-11-15 | 2012-10-24 | 山东农业大学 | Culture medium for producing antifungal substance by streptomyces fermentation and preparation method thereof |
| CN103242105B (en) * | 2013-05-23 | 2015-03-18 | 黄毅 | Multi-antibiont organic fertilizer and preparation method thereof |
| CN105875113A (en) * | 2014-10-20 | 2016-08-24 | 董金锋 | High-yield cultivation method of cotton |
| CN104396486B (en) * | 2014-10-27 | 2016-06-22 | 山东省农作物种质资源中心 | A kind of implantation methods of winter wheat |
| CN104920107B (en) * | 2015-07-03 | 2017-05-17 | 云南农业大学 | Method for manufacturing nutrition pot body for controlling bacterial wilt of flue-cured tobaccos by utilizing organic solid wastes |
| CN105993461B (en) * | 2016-05-20 | 2021-12-31 | 甘肃省科学院生物研究所 | Seedling raising method of angelica sinensis microbial agent and prevention and treatment method of soil-borne diseases |
| CN106831116B (en) * | 2017-01-05 | 2020-06-02 | 商丘师范学院 | Slow-release compound pesticide fertilizer and application thereof |
| CN107879852A (en) * | 2017-11-29 | 2018-04-06 | 大工（青岛）新能源材料技术研究院有限公司 | A kind of edaphon modifying agent and preparation method thereof |
| CN108083920A (en) * | 2017-12-29 | 2018-05-29 | 大工（青岛）新能源材料技术研究院有限公司 | Microbial manure and its preparation method and application |
| CN107988120B (en) * | 2017-12-29 | 2021-04-30 | 大工（青岛）新能源材料技术研究院有限公司 | Microbial agent and preparation method and application thereof |
| CN108391554A (en) * | 2018-03-27 | 2018-08-14 | 佛山市三水区嘉信农业技术研究院（普通合伙） | A kind of fig method for non-polluted cultivation rich in various trace elements |
| CN108821860A (en) * | 2018-07-06 | 2018-11-16 | 句容亚达生物科技有限公司 | It is a kind of can mitigate soil-borne disease, growth-promoting root, anti-continuous cropping bacteria preparation |
| CN111362742A (en) * | 2018-12-26 | 2020-07-03 | 王虎 | Preparation method of special nutrient fertilizer for vegetable planting |
| CN110256145A (en) * | 2019-06-21 | 2019-09-20 | 广西壮族自治区烟草公司百色市公司 | A kind of tobacco organic fertilizer and preparation method thereof |
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